@1531497176.59CEST = =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =tquick =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #Loading /home/esp/esp2/mission/stdMER2017.rb mission .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0101h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*`  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0101h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +128 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +625 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0103h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*>  +130 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*I  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0103h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0105h.pcr #Building PCR Slug from extSrc with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*4 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*7  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*;  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +22 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +21 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0105h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +64 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +129 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +625 h 'f .t\ATV.dial :waste F*\ n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0108h.pcr #Building PCR Slug from extSrc with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +129 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*+  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +21 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +22 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0108h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +87 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +21 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +21 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +31 h  ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*o  +108 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +423 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2532 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +128 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +626 h ' M .t\ATV.dial :waste F*r n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3335 h 'R .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*x'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*y  +21 h F*{% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *|' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*z  +129 h  F*v% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *'J +1826 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*}  +129 h F*% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *' ~ +626 h ' ~ .t\ATV.dial :waste F*~ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F*% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\ % h % .t\AS.seek 0.31ml F* 'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F* % h %m\  .t\AS.seek 0.335ml F*' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +107 h  F*(% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5054 h '(@ .t\ATV.dial :extSrc F*+  +107 h  F*,% h %(@ .t\AS.seek 0.02ml F*' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F*/ % +43 h % F*'% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*1% h % .t\AS.seek 0.31ml F*0'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*4% h %m\  .t\AS.seek 0.335ml F*5' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h % .t\AS.seek 0.32ml F*8'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*9  +43 h  .t\AS.seek 0ml F*6'V +6456 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *:'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *A'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *?'V +10076 h 'V .t\ATV.dial :air2 F*F  +108 h  F*B% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *D'(@ +5053 h '(@ .t\ATV.dial :extSrc F*C  +108 h  F*E% h %(@ .t\AS.seek 0.02ml F*K' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*H n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +145 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +32 h 'V .t\ATV.dial :air3 F*N % +43 h % F*L% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*M% h % .t\AS.seek 0.31ml F*S'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % .tdelay 3 seconds +300 F*V% h %m\  .t\AS.seek 0.335ml F*O' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*Y% h % .t\AS.seek 0.32ml F*U'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*T  +43 h  .t\AS.seek 0ml F*X'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *W'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *`'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *a'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *_'V +10077 h 'V .t\ATV.dial :air2 F*\\  +108 h  F*d% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *b'(@ +5053 h '(@ .t\ATV.dial :extSrc F*f  +108 h  F*e% h %(@ .t\AS.seek 0.02ml F*i' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*g n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +146 h 'R #m\EXIT @1531497230.07CEST @1531570855.11CEST = =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =tquick =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 11:07:59CET01-Jan-70 .delayUntil "11:07:59.00" +3647899 #Loading /home/esp/esp2/mission/stdMER2017.rb mission .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*] n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*` I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*w  +22 h ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +129 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +626 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0113h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*>  +129 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +21 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*I n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*J I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0113h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0115h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*4 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*7 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*; % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +107 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +22 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0115h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +21 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +129 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +626 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0118h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +129 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*+ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +21 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0118h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2531 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +625 h ' M .t\ATV.dial :waste F*r n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3334 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0120h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*y  +130 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0120h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +21 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +31 h  ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +21 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*X  +108 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +620 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R F*b% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0122h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*f  +129 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *g'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +21 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*k n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*q I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*x % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET85893.77 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0122h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*C  +107 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*H  +129 h  F*J% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *K'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*N  +129 h F*I% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *L' ~ +625 h ' ~ .t\ATV.dial :waste F*G n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +32 h 'V .t\ATV.dial :air3 F*S % +43 h % F*R% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*P% h % .t\AS.seek 0.31ml F*Y'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % .tdelay 3 seconds +300 F*X% h %m\  .t\AS.seek 0.335ml F*Z' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*[% h % .t\AS.seek 0.32ml F*`'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*W  +43 h  .t\AS.seek 0ml F*a'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *^'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *_'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *f'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *i'V +10077 h 'V .t\ATV.dial :air2 F*d  +108 h  F*g% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *l'(@ +5053 h '(@ .t\ATV.dial :extSrc F*c  +108 h  F*j% h %(@ .t\AS.seek 0.02ml F*m' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*h n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +31 h 'V .t\ATV.dial :air3 F*k % +43 h % F*t% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *s' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*r% h % .t\AS.seek 0.31ml F*p'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % .tdelay 3 seconds +300 F*y% h %m\  .t\AS.seek 0.335ml F*u' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*w% h % .t\AS.seek 0.32ml F*{'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*v'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +107 h  F*\ % h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +108 h  F* % h %(@ .t\AS.seek 0.02ml F* ' +4851 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F* % h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.31ml F*'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*"' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*(  +43 h  .t\AS.seek 0ml F*'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +10077 h 'V .t\ATV.dial :air2 F*2  +107 h  F*'% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *%'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +107 h  F*1% h %(@ .t\AS.seek 0.02ml F*0' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*3 n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +145 h 'R #m\EXIT @1531570923.49CEST @1531750000.78CEST = =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =tquick =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi@; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 01:53:36CET02-Jan-70 .delayUntil "01:53:36.00CET02-Jan-70" @CET89616.00 #Loading /home/esp/esp2/mission/stdMER2017.rb mission .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0202h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*`  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +108 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi@; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0202h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +128 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +625 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0204h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*>  +130 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*I  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*J  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*P  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +108 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0204h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0206h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*4  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*7  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*;  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +108 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +21 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0206h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +128 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +625 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0209h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +22 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0209h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2531 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +626 h ' M .t\ATV.dial :waste F*r n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3334 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0211h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*y  +130 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0211h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +64 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +21 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*X  +107 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R F*b% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0213h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*f  +129 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *g'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*k  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*q  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*x  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0213h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*C  +108 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +423 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*H  +129 h  F*J% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *K'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*N  +129 h F*I% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *L' ~ +625 h ' ~ .t\ATV.dial :waste F*G n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +32 h 'V .t\ATV.dial :air3 F*S % +43 h % F*R% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*P% h % .t\AS.seek 0.31ml F*Y'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % .tdelay 3 seconds +300 F*X% h %m\  .t\AS.seek 0.335ml F*Z' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*[% h % .t\AS.seek 0.32ml F*`'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*W  +43 h  .t\AS.seek 0ml F*a'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *^'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *_'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *f'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *i'V +10077 h 'V .t\ATV.dial :air2 F*d  +108 h  F*g% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *l'(@ +5053 h '(@ .t\ATV.dial :extSrc F*c  +108 h  F*j% h %(@ .t\AS.seek 0.02ml F*m' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*h n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +32 h 'V .t\ATV.dial :air3 F*k % +43 h % F*t% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *s' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*r% h % .t\AS.seek 0.31ml F*p'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % .tdelay 3 seconds +300 F*y% h %m\  .t\AS.seek 0.335ml F*u' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*w% h % .t\AS.seek 0.32ml F*{'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*v'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +108 h  F*\ % h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5053 h '(@ .t\ATV.dial :extSrc F*  +108 h  F* % h %(@ .t\AS.seek 0.02ml F* ' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F* % h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % .t\AS.seek 0.31ml F*'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*"' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*(  +43 h  .t\AS.seek 0ml F*'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +10077 h 'V .t\ATV.dial :air2 F*2  +107 h  F*'% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *%'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +107 h  F*1% h %(@ .t\AS.seek 0.02ml F*0' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*3 n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +146 h 'R #m\EXIT @1531750069.11CEST @1531920810.46CEST = =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =tquick =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi`; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:39:15CET02-Jan-70 .delayUntil "16:39:15.00CET02-Jan-70" @CET142755.00 #Loading /home/esp/esp2/mission/stdsingleMER2017.rb mission .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0216h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*] n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*` I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi`; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0216h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*w  +22 h ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +129 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +626 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R !"MFBPCR"o:NoMethodError :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message1undefined method `fetch' for :carrier:Symbol: name:\ fetch:@timestampu: Time R=:@checkpoint: omitted: args[:masterMix .BadNews.suppressMail "undefined method `fetch' for :carrier:Symbol",Subject: "NoMethodError in MFBPCR" !to:Thread::ChildDied : @causeo:NoMethodError :bt[";/home/esp/esp2/protocol/pcrslug.rb:175:in `pcrMission'"6/home/esp/esp2/lib/threads.rb:57:in `recoverable'";/home/esp/esp2/protocol/pcrslug.rb:170:in `pcrMission'" pcrDecon !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> MFB.ready! !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> exit #m\EXIT @1533710592.54CEST @1533735287.80CEST = =mquickScheduler =tquick =ECan =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refil; : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #Loading /home/esp/esp2/mission/18tun.rb mission .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:34:56, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:34:56, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:35:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R  'Q h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:35:05CEST08-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:35:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe .\AFV.select :holdingCoil h & F* M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&+1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml C'/'4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9  0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:24:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:24:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:24:36, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'Q .t\CBV.dial CSV C'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 09:24:41CEST09-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:24:36, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% h % E ;3\\0\\0 ."airStroke"\CS.seek 0ml #t\Calibrating pressure and temperature sensors C'/'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9  0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:09:37, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:16 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:09:37, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:16" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:09:42, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'<& h &W !to:Slide::Error\ :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:@axisName"Collection Clamp: mesg"Collection Clamp not Open: @reply0:@timestampu: Time ^D .BadNews.suppressMail "Collection Clamp not Open",Subject: "Slide::Error in quick" #Waiting to Resume from Checkpoint "Loading FlushPuck into Collection Clamp from FlushPuck::Garage" !o:Interrupt:\ signoi:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:25:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:17 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:25:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:17" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:25:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'<& h &W !to:Slide::Error\ :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:@axisName"Collection Clamp: mesg"Collection Clamp not Open: @reply0:@timestampu: Time F .BadNews.suppressMail "Collection Clamp not Open",Subject: "Slide::Error in quick" #Waiting to Resume from Checkpoint "Loading FlushPuck into Collection Clamp from FlushPuck::Garage" !o:Interrupt:\ signoi:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &_ A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> execute "18tun" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:52:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 ."shutdown"\AS.coast #t\Asleep til +0:00:15 ."shutdown"\SS.coast F*5 #t\Continuing at 01:02 S)5 ."shutdown"\CC.coast E ;3\\0\\0 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:53:39, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:02" ."shutdown"\Forearm.coast P%6 ."shutdown"\Elbow.coast M&5 ."shutdown"\SC.coast .\SE.coast A(6 + M&6 A(5 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown +1470 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )M h  C' 'N h  P% %O h  F* *P h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *Q  h  .t\SPE.configure SPEconfig F*\ 2 *R% h %R .t\AS.configure ASconfig F*#,,'T(^20( b S)S% h % .t\SS.configure SSconfig C'T& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:53:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&|1 +150 h 1 M&}& h &_ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C' (W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'"  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'(  '* h +75 .t\CTV.dial :intake C'+  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C','4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'%% h %4 .t\CSV.dial :flush C''  +76 h  .t\CS.seek 5ml C'/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'1% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :air .\CS.seek 0ml C'4'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +506 h '0 .t\CSV.dial :waste C'6 +38 h .t\CS.seek 0ml C'7'4 +506 h '4 C'8& h &W C'9% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 3ml C';'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'=% h %0 .t\CS.seek 5ml C'>'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'?'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +507 h '0 .t\CSV.dial :waste C'A +75 h .t\CS.seek 0ml C'B'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&C& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +6 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'I( +416 h ( M&H& h &ӷ M&K% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &$(- +265 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +90 h 'η .tHand.open M&)1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 09:53:48CEST10-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:53:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*.% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'-& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&M% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(N% h %X A(O. h ., A(P& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (Q& h &  M&R& h &- M&S% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &T( +7 F*U% h %&K ."readyMFB"\ATV.dial :waste2 F*V % +107 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *W' +52 h ( M&X& h & A(Y& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A([& h &  .t\SE.seek 20.71 Pucks below Top Plate A(\\' +56 h ' A(]& h &  M&^& h & .t\Elbow.seek 2 M&_( +7 h ( M&`& h & A(b& h &  .t\Forearm.seek 2 + 300 counts M&c'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &e/ +150 h / ."downElevator"\SE.seek :down M&g& A(f'X h & M&a% h %; .t\Forearm.seek :retract M&k'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&n(ӷ +142 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'w(W +416 h (W .tHand.open M&x1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'q& h &W ."airStroke"\CSV.dial :waste S)s% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'{'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '?'4 +2609 h '4 C'A% h %4 S)@% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'C @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'9 h 9Td +100 C'9 h 9kd +100 C'z9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'\ 9 h 96d +100 C' 9 h 9Ld +100 C'u9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9d +100 C'9 h 9(d +100 C'+9 h 9>d +100 C'9 h 9Td +100 C'*9 h 9id +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'%9 h 9d +100 C',9 h 9d +100 C'09 h 9d +100 C'9 h 9  d +100 C'19 h 9 d +100 C'/9 h 9 *d +100 C'29 h 9 ?d +100 C''9 h 9 Td +100 C'39 h 9 j d +100 C'69 h 9 $d +100 C'59 h 9 (d +100 C'89 h 9 ,d +100 C'99 h 9 0d +100 C'79 h 9 4d +100 C'<9 h 9 8d +100 C'=9 h 9 9 h 9\ &Dd +100 C'49 h 9\ :Hd +100 C'A9 h 9\ OLd +100 C'@9 h 9\ cPd +100 C'C9 h 9\ xTd +100 C':9 h 9\ Xd +100 C'F9 h 9\ \\d +100 C'G9 h 9\ `d +100 C'9 h 9\ dd +100 C'E9 h 9\ hd +100 C'9 h 9\ ld +100 C'H9 h 9 pd +100 C'K9 h 9 td +100 C'#9 h 9 /xd +100 C' 9 h 9 C|d +100 C'D9 h 9 Wd +100 C'?9 h 9 kd +100 C'J9 h 9 d +100 C'9 h 9 d +100 C')9 h 9 d +100 C'L9 h 9 d +100 C'.9 h 9 d +100 C' 9 h 9 d +100 C'M9 h 9 d +100 C'-9 h 9 d +100 C'N9 h 9 d +100 C'O9 h 9 1d +100 C'P9 h 9 Dd +100 C'R9 h 9 Xd +100 C'Q9 h 9 kd +100 C'U9 h 9 ~d +100 C'S9 h 9 d +100 C'X9 h 9 d +100 C'Y9 h 9 d .t\CH.hold 5:00,85C C'V   P h  +30000  C'[9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'&'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'] @ +113 h @ .t\CSV.dial :air C'$ @ +38 h @ .t\CS.seek 5ml C'b'p +1372 h 'p .t\CSV.dial CTV C'!  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'e @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'g  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'a @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'o'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'T +113 h .t\CBV.dial CSV C'Z  +19 h C'l% h %4 .t\CS.seek 5ml C't'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'c  +38 h C'\\% h %p .t\CS.seek 7ml C'f'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'_ @ +37 h @ .t\CS.seek 10ml C'k' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'I ` +132 h ` #tLysing Complete C'x& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&d% h %\  A(q% h %X A(s. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (}( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (v& h & M&p& ."raiseStack"\SE.seek :upperLimit h &ӷ A(i'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'r( +23 h 'Z +392 ( A(& h & A(|& h & M&& h &ӷ M&W% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &z'Y +92 h 'Y .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %Y A(~& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' A( % h %Z ."downElevator"\SE.seek :down A(u'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'" { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'(& h & .tHand.open M&1 +2 h 1 M&+& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&*(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&%/ +150 h / M&,& h &- M&0% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +265 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'6& h &W .tHand.open M&51 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%7 +150 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%=' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +37 h  .t\PS.seek 0ml P%>' +1598 h ' .t\PSV.dial PSR P%4 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%A' +2609 h ' ."connect"\PRV.dial CBV P%@  .t\CBV.dial PRV C'C  h +75 .t\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%F  +113 h .t\PS.seek 0ml P%G' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%E' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%H' +2610 h ' .t\PSV.dial :air P%K  +37 h  .t\PS.seek 2.5ml P%#' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%D' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&?& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C' ( +416 h ( M&M& h &ӷ M&-% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Q'η +91 h 'η .tHand.open M&U1 +150 h 1 M&S% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'Y& h & M&V% h %\  A([% h %X A(^. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (]( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ($& h &  M&&& h &- M&!% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &e( +166 h ( M&g& h & A(a& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &b' +90 h ' A(T& h &  .t\SE.seek 19.71 Pucks below Top Plate A(Z' +66 h ' A(l& h &  M&o& h & .t\Elbow.seek 2 M&c( +6 h ( M&\\& h & A(t& h &  .t\Forearm.seek 2 + 300 counts M&_'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &`/ +150 h / ."downElevator"\SE.seek :down M&f& A(I'X h & M&x% h %; .t\Forearm.seek :retract M&d'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&q(ӷ +143 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&j1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'm  +150 h  .t\CSV.dial CTV C'w  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'|' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%W  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%{  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%B +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'~ @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'z'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'i& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A("% h %X A((. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (+& h & M&& h &ӷ M&\ % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(u& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +90 h ' A(k& h & .t\SE.seek -0.29 Pucks below Top Plate A( '\\ +264 h '\\ M&%& h & A(,& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &0'Y +25 h 'Y .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(/' +27 h ' M&2% h %Y A(*& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&r& h & A(6& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &5' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(8' +37 h ' M&7& h & M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +89 h '\  .t\Elbow.seek CC M&;(ӷ +142 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'C( +416 h ( A(:& h & A(F& h & M&A& h &ӷ M&% h % .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&E& h & A(K& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &H'Y +92 h 'Y .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b  +150 h 1 M&?% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &)'υ +21 h 'υ M&.& h & A(1& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &9' +22 h ' A(3% h % ."downElevator"\SE.seek :down A(M'X +100 (-% h %S M&L% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +90 h '\  +60 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%R  +2 A(6 +111 h  P%J% h % .t\PS.seek 0.499ml P%U' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%S +38 h .t\PRV.dial :air P%N  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%Y  +38 h .t\CBV.dial AIV C'V  +38 h .t\PRV.dial between CBV and air P%[  +19 h  .t\PRV.dial :air P%^  +18 h  P%$% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %&' +251 h ' .t\PRV.dial :waste2 P%! @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %Q' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&a1 +2 h 1 M&T& h & M&O% h %\  .t\Elbow.seek FlushPuck::Garage M&l(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&t'η +91 h 'η .tHand.close M&X/ +150 h / M&`& h &- M&f% h %η .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &e(ӷ +264 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'Z& h &W .tHand.open M&I1 +150 h 1 M&c% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  C' & h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%j @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%m  +76 h  .tdelay 2 seconds +200 P%w% h % .t\PS.seek 1.499ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%]' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%| +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%W  +38 h  .tdelay 2 seconds +200 P%D% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%{  +76 h .tdelay 2 seconds +200 P%B% h %r .t\PS.seek 0.475ml P%' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%~'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%i  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%" +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%(  +75 h P%+% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'\ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %u'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'k { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %q' +1312 h ' .t\PSV.dial :air P%n  +38 h  .t\PS.seek 2.5ml P%%' +2610 h ' .t\PSV.dial :PML P%, +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '2& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'*& F*r @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*6  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%7 +22 h  ."MFBPCR"delay 20 seconds +91 h P%<% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%}' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *0'6 +66 .t\PSV.dial :waste1 P%=  +38 h  .t\PS.seek 0ml P%4' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%/' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%5 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +22 h ' .t\PSV.dial PSR P%' +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*:'ͯ +92 h P%F% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%A' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%K  +37 h  .t\PS.seek 0ml P%>' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*;  +21 h  F*#% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *?' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%.' +217 ."MFBPCR"\ATV.dial :AIV F*1 I +43 h I F*3% h % ."MFBPCR"\AS.seek 0.469ml F*G' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%-' +2610 h ' .t\PSV.dial PSR P%L +113 h P%H% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%)' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +38 h  .t\PS.seek 0ml P%R' +739 h ' ."MFBPCR"delay 5 seconds +500 F*J9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*9 n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*M% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%S' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%N +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Y' +2609 h ' .t\PSV.dial PSR P%V +113 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +37 h  .t\PS.seek 0ml P%U' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%! +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%&' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%a +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %T'k +2679 h 'k .t\PSV.dial :waste1 P%O  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %o' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*\\ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b * 'V +849 h ' .t\PSV.dial :air P%X  +113 h  .t\PS.seek 2.5ml P%`' +2610 h ' .t\PSV.dial :mixloop P%f +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %P' +5106 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %b' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *x'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*C I +43 h I F*Z% h %Q ."MFBPCR"\AS.seek 0.01ml F*I' +171 h ' .t\PSV.dial :mixloop P%c +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb % ' +619 h ' ."MFBPCR"\ATV.dial :waste F*j n +128 h n ."MFBPCR"\AS.seek :empty F*v'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*m% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *w' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*Q  +43 h F*p% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C']  +37 h  C'|% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'D' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'{  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'~% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F* % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'i' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'"'4 +2647 h '4 C'(& h &W #tGaraging FlushPuck in Collection Clamp M&+& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'u( +416 h ( M&n& h &ӷ M&q% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &%(- +264 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&71 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'& h & M& % h %\  A(=% h %X A(h. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (}( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&4& h &- M&5% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &/( +166 h ( M&@& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &'' +89 h ' A(F& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(0' +77 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&:( +7 h ( M&K& h & A(;& h &  ."WCR"\Forearm.seek 2 + 300 counts M&#'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +150 h / ."downElevator"\SE.seek :down M&E& h & A('X M&1% h %; ."WCR"\Forearm.seek :retract M&3'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&>(ӷ +143 h (ӷ M&?& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +90 h ' ."WCR"\CC.seek :closed C'L(W +4 ."MFBPCR"\ATV.dial :waste2 F*H % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +369 h (W ."WCR"Hand.open M&81 +150 h 1 M&)% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*9  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *M' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)G+ +7 h + S)% h % C'R& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'N  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X .t\CBV.dial CSV C'J  +38 h +75 .t\CTV.dial :air C'Y  +151 h .t\CS.seek 0ml C'^'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +506 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'M'4 +506 h '4 #t\Flushing upper manifold C'\\& h &W C'o% h %4 .t\CSV.dial :flush C'f  +38 h  .t\CS.seek 5ml C's'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'}% h %p .t\CS.seek 7ml C'$'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'l'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'g +38 h .t\CS.seek 0ml C'O'4 +507 h '4 C'X& h &W C'U% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.seek 3ml C'P'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +37 h @ C'`% h %0 .t\CS.seek 5ml C'b'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'I'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +506 h '0 .t\CSV.dial :waste C'Q +76 h .t\CS.seek 0ml C'p'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'Z& h &W C' % h %4 .t\CSV.dial :flush C'|  +38 h  .t\CS.seek 5ml C'x'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C']% h %p .t\CS.seek 7ml C'B'i +416 h 'i ."connect"\CSV.dial CTV C'z  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'C @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C'% h %4 .t\CSV.dial :flush C'~  +75 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'm% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'T  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'D  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &"(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +91 h 'η .tHand.open M&_1 +150 h 1 M&n% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&21 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &7(- +6 h (- M&e& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.seek :garage M&,'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&<% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +91 h ' .t\CC.seek :closed C'4(W +416 h (W C'5& h &W .tHand.open M&@1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C';  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '%% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'A'[ +462 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +2010 h '4 #t\Purging external Intake C'1& h &W C'/% h %4 .t\CSV.dial :flush C'#  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +37 .t\CTV.dial :intake C'?  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'0'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'H  +75 h #t\Flushing upper manifold C'& h &W C'3% h %4 .t\CSV.dial :flush C':  +76 h  .t\CS.seek 5ml C')'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'N% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'- +113 h .t\CTV.dial :air .\CS.seek 0ml C'.'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'[ +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C'>& h &W C'Y% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.seek 3ml C'^'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'a% h %0 .t\CS.seek 5ml C'\\'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'o'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C'M +75 h .t\CS.seek 0ml C'V'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&}& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&g/ +150 h / .t\CC.seek :open C't( +416 h ( M&X& h &ӷ M&U% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &P'η +91 h 'η .tHand.open M&b1 +150 h 1 M&I% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Completed [coreNegNTCphase] begun 01:03:00 #\Extending 18tun until completion of MFBPCR +256961 !"PCR"o:#AnalyticalModule::PCRRawSample: @refip; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*B'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0104h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*6 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*A' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*1  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.536ml F*#'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*'  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*? n +21 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*0  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*N% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0104h.pcr F*.% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *G'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*f% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F*g% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*s % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *I' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*P% h % ."MFBPCR"\AS.seek 0.81ml F*r'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +21 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0107h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F*D'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*k  +22 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*+  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*_' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*n  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +107 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.6ml F*&'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*d  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F**'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0107h.pcr F*h% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*A  +64 h  F*'% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +21 h F*-% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*}  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*s  +21 h ."MFBPCR"delay 2 seconds +200 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*c I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*P% h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*j n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*r' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*|  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +108 h  ."MFBPCR"delay 7 seconds +700 F*Z% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*B  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*~'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h.pcr F*m% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\ 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +86 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*<% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +21 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F**'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*5  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ARV.dial :carrier F*1  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*#'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*0  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *3'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*: n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*-% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*S I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*9' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*^'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*f  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*o  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*g'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h.pcr F*$% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*4 % +87 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *`'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +21 h F*i% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*p  +65 h  F*r% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *]'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +31 h  ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*B% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*~'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0114h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*= n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*,  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.536ml F**'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*/% h % \\ ."MFBPCR"\AS.seek 0.326ml F*''{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0114h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*A @@T ."MFBPCR"\ASV.dial :SPEdelivery F*#  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*?  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *H' +424 h ' ."MFBPCR"\ATV.dial :ASV F*0 n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*8  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*:'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*- n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *S'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*.'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *>' +424 h ' ."MFBPCR"\ATV.dial :air3 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*)% h % ."MFBPCR"\AS.seek 0.71ml F*!' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h % H ."MFBPCR"\AS.seek 0.735ml F*\\' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*^% h % Ű ."MFBPCR"\AS.seek 0.72ml F*f' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*o n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*M' +7162 h ' ."MFBPCR"\ATV.dial :waste F*} n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*$ % +43 h % F*g% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*U% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %  ."MFBPCR"\AS.seek 0.604ml F*`'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*P% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*l' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*j n +86 h n ."MFBPCR"\ASV.dial :wasteR F*i  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*r % +107 h % F*I% h %V ."MFBPCR"\AS.seek 0.01ml F*]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*W' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h % q ."MFBPCR"\AS.seek 0.605ml F*{'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*B% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *m'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *~'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*D n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *R'R +202 h 'R ."MFBPCR"\SPE.coast F*k  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*w'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*\ n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*n  +43 h  F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*(  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *2'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*e n +64 h n F*q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *u' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *<': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*,% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *h'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*d n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *@' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b **': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*F n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*; n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*6  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*K% h % ."MFBPCR"\AS.seek 0.96ml F*1'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %0 ."MFBPCR"\AS.seek 0.985ml F*'' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*E'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*0 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *-'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *3'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*S  +42 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *.' +9987 h ' ."MFBPCR"\ATV.dial AIV F*9 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*>' +9880 h ' ."MFBPCR"\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.seek 0ml F*)'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*[% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*o% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +21 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*M'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +21 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*U  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*i  +31 h  ."MFBPCR"\ATV.dial :air3 F*l % +87 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*p'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*]'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*x% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *~'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*R % +108 h % ."MFBPCR"\AS.seek 0ml F*\ 'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0117h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*+'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*e  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*2' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.53ml F*&' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*"'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*d I +107 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*5  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*F  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*;'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0117h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*''n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*-% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *:'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Y  +32 h  ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*f% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*g' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0119h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ARV.dial :carrier F*`  +10 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*l'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*|  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *p'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*C % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*W% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*z  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.65ml F*D' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*~  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0119h.pcr F*+% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*&  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +31 h  ."MFBPCR"\ATV.dial :air3 F*5 % +87 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*;'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0122h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ARV.dial :carrier F*8  +11 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*[  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *.'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*J  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*)' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*G  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*a' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*^  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.536ml F*o'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*g I +107 h I ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*U  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*s  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*b  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*P'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*i % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET83142.44 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0122h.pcr F*c% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*j  +21 h ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *|'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*I'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*p'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*W% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*{  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *z'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*D% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0200h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *@'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*A n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.536ml F*;'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*K% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*# I +108 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*?'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*E  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*'  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*-'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0200h.pcr F*H% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*3  +22 h ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Y'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +21 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*\\% h %n ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*f  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*X  +32 h  ."MFBPCR"\ATV.dial :air3 F*4 % +87 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*`'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*P'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*L% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0202h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*]  +11 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*D% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*\ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*_ I +108 h I ."MFBPCR"delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0202h.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*d % +86 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *h'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*/  +64 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *%'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*E% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *'' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*0% h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*-'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*N% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*S  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0205h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ARV.dial :carrier F*)  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*g n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*X  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +21 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *U'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*b' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*i  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*c% h % ."MFBPCR"\AS.seek 0.53ml F*`' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*j  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.536ml F*P'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*l  +22 h  ."MFBPCR"delay 7 seconds +700 F*r% h %< ."MFBPCR"\AS.seek 0.594ml F*Z'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.65ml F*C' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*W  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0205h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h %Z ."MFBPCR"\AS.seek 0.81ml F*T'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*w% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +31 h  ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*u% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +5060 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:54:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 3:00.811548 !o: EOFError:bt[ "1/opt/mbari/lib/ruby/1.8/irb.rb:72:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"end of file reached !o: EOFError:bt[ "1/opt/mbari/lib/ruby/1.8/irb.rb:72:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"end of file reached !o:SignalException :\ signoi:bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" SIGHUP:@timestampu: Time Fv^] !o:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error #m\EXIT @1533890995.32CEST @1534504772.61CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 07:23:21CET02-Jan-70 .delayUntil "07:23:21.00CET02-Jan-70" @CET109401.00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:19:40, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 07:23 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:19:57, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 07:23" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:20:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 13:20:05CEST17-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:20:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % C'/'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&[% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&s& h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &['Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&{'υ +21 h 'υ M&~& h & A(Z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& F*& @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*`  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *['6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*$'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F**% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'/  '7  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'L& h & M&@% h %\  A(g% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (1( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h &  M&^& h &- M&_% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +166 h ( M&j& h & A(4& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(p& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A([' +77 h ' A(K& h &  M&;& h & ."WCR"\Elbow.seek 2 M&d( +6 h ( M&u& h & A(e& h &  ."WCR"\Forearm.seek 2 + 300 counts M&x'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &k/ +150 h / ."downElevator"\SE.seek :down A(o'X M&q& h & M&Z% h %; ."WCR"\Forearm.seek :retract M&]'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' ."WCR"\CC.seek :closed C'v(W +3 ."MFBPCR"\ATV.dial :waste2 F*t % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&b1 +150 h 1 M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +6 h + S)8% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *E' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*9% h % ."MFBPCR"\AS.seek 0.279ml F*D'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*H n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*G % +43 h % F*!% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *R'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*S I +21 h I F*=% h %v ."MFBPCR"\AS.seek 0.059ml F*P'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*"% h % ."MFBPCR"\AS.seek 0.769ml F*%' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h % q ."MFBPCR"\AS.seek 0.794ml F*Q' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*a% h % k ."MFBPCR"\AS.seek 0.779ml F*f'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*T I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *L' +4416 h ' ."MFBPCR"\ATV.dial :waste F*@ n +128 h n ."MFBPCR"\AS.seek :empty F*g'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*U% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*`% h % ."MFBPCR"\AS.seek 0.579ml F*j' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*;% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +85 h n ."MFBPCR"\ASV.dial :wasteR F*V  +43 h  ."MFBPCR"\AS.seek 0ml F*k'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Z'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*Y % +107 h % F*X% h %V ."MFBPCR"\AS.seek 0.59ml F*i' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*C n +107 h n ."MFBPCR"\ASV.dial :wasteR F*x% h %  ."MFBPCR"\AS.seek 0.09ml F*[' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*o I +86 h I F*d% h % ."MFBPCR"\AS.seek 0.12ml F*]' +625 h ' ."MFBPCR"\ATV.dial :air3 F*) % +22 h % F*I% h % ."MFBPCR"\AS.seek 0.13ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*c% h % ."MFBPCR"\AS.seek 0.23ml F*8' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*~'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*1% h %X ."MFBPCR"\AS.seek 0.24ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*\  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*M I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*#% h %(A ."MFBPCR"\AS.seek 0.6ml F*s'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*'% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*? % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*(  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0208h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *,'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*.' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*l % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*7'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*F% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*9' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*E  +43 h  ."MFBPCR"\ASV.dial :waste1 F*H  +64 h ."MFBPCR"\ARV.dial :air1 F*D  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*!'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*S % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254541 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'=  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'R @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)P% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'B' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'\\  ."WCR"\CSV.dial CTV C'%  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'N'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'a  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Q' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'T  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +37 h  C'@% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C' ' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'U' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'L @ +76 h @ +50 S)g% h % ."WCR"\CBV.dial between CSV and waste2b C'_  +94 h  C'`% h % ."WCR"\CSV.dial between air and CTV C'^ ` +56 h ` ."WCR"\CS.seek 7.288ml C'4' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'p  ."WCR"\CSV.dial CTV C'j  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C';  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'e  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'V  +38 h  C'u% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'k' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'Y @ +75 h @ +50 S)X% h % ."WCR"\CBV.dial between CSV and waste2b C'Z  +94 h  C'C% h % ."WCR"\CSV.dial between air and CTV C'x ` +57 h ` ."WCR"\CS.seek 7.288ml C'i' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C't  ."WCR"\CSV.dial CTV C'o  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'[  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ')' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +150 h C']& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&{% h %\  A(c% h %X A(b. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&8& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &1( +142 h ( M&~& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\ ' +89 h ' A(r& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(w'K +254 h 'K M& & h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &M'Y +25 h 'Y ."WCR"\Elbow.hold M& , +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- h -+K M&$&.,(\ #b ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&}% h %Y A(& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'υ +21 h 'υ M&& h & A(#& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M&s& h & M&'% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&?(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'z( +416 h ( A(& h & A(& h & M&& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&5'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &m( +142 h ( M&& h & A(*& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &01 +150 h 1 M&,% h %Y A( & h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&/'υ +21 h 'υ M&& h & A(l& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A(.% h % ."downElevator"\SE.seek :down A(A'X +100 (F% h %B M&7% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&21 + A(6 + h 1 M&>& h & M&<% h %\  .t\Elbow.seek FlushPuck::Garage M&J(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.seek :garage M&D'η +91 h 'η .tHand.close M&G/ +150 h / M&9& h &- M&6% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +264 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'& h &W .tHand.open M&"1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'%& h &W C'\\% h % .t\CSV.dial CSR C'B  +37 h  C'a% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'N'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'T% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C'@ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Q'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'L% h %4 .t\CSV.dial :flush C'g  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '_'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'^% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CTV C'j  .t\CTV.dial CSV C'p  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +75 h @ .t\CS.seek 0ml C';'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'e% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'u'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'k% h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  +38 h  .t\CBV.dial :waste2b C'Z  +75 h  .t\CS.seek 0ml C'C'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'x  +37 h  C'q% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'o'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  +37 h  .t\CBV.dial :waste1 C'[ @ +76 h @ .t\CS.seek 0ml C'd'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'I  +38 h  C']% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '{'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +709 h ' ."connect"\CSV.dial CTV C'b  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'8'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h  'y +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'} +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'& h &W C'#% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'1% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C' & h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'*'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'?% h %4 .t\CSV.dial :flush C'0  +37 h  .t\CS.seek 5ml C','p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'l'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  '5  +38 h  +113  .t\CBV.dial :waste1 C'm @ +37 h @ .t\CS.seek 0ml C'F'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'7& h &W C':% h %4 .t\CSV.dial :flush C'2  +76 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'n  +76 h  .t\CS.seek 0ml C'E'4 +765 h '4 #tGaraging FlushPuck in Collection Clamp M&H& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &A(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&G' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C'6( +416 h ( M&3& h &ӷ M&D% h % .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&1 +150 h 1 M&"% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'%& h & .tHand.open M&\\1 +2 h 1 M&B& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &a(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &- .t\Forearm.seek :garage M&T'η +91 h 'η .tHand.close M&N/ +150 h / M&@& h &- M&f% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +264 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'_& h &W .tHand.open M&j1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'U& h &W C'K% h %4 .t\CSV.dial CSR C'e  +37 h  C'V% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ';'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'k'[ +463 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +2009 h '4 #t\Purging external Intake C'Z& h &W C'Y% h %4 .t\CSV.dial :flush C'x  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'C .t\CBV.dial CSV C'o  +37 h +38 .t\CTV.dial :intake C'i  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'['4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C't  +75 h #t\Flushing upper manifold C'I& h &W C']% h %4 .t\CSV.dial :flush C'd  +75 h  .t\CS.seek 5ml C'{'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +37 h @ C'% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'h'4 +507 h '4 C'y& h &W C' % h %4 .t\CSV.dial :flush C'r  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'#'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'\ '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&1& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +7 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'(( +416 h ( M& & h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [coreNEG] begun 07:23:52 #\Extending 18tun until completion of MFBPCR +256961 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0208h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*?  +21 h ."MFBPCR"\ATV.dial :air3 F*0 % +87 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*,% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*l'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*9  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*D% h % ."MFBPCR"\AS.seek 0.81ml F*G'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +21 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0211h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*Q  +10 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*_ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*K  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *V'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*` I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*W' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*; % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.53ml F*k' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Z  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.536ml F*x'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*X  +21 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*C'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*t'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*b  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*c % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0211h.pcr F*~% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*8  +22 h ."MFBPCR"\ATV.dial :air3 F*y % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *r'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +21 h F*}% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*#'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*s% h %n ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *L'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*?'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F**% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0213h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*2  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*n'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*H n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*=  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *3'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*D  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*P% h %Fj ."MFBPCR"\AS.seek 0.524ml F*J' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*S  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*"  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*R  +108 h  ."MFBPCR"delay 7 seconds +700 F*\\% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.6ml F*z'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*N  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*g% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0213h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*6  +21 h ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *K'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*p% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*;'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*W% h %n ."MFBPCR"\AS.seek 0.82ml F*Z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*k  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *o'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*t  +32 h  ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*c'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*~% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0215h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*1  +10 h  F*L% h %V ."MFBPCR"\AS.seek 0.5ml F*4'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*&' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*0  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*?'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*m'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*5  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0215h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*H  +22 h ."MFBPCR"\ATV.dial :air3 F*/ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*+% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*G% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*A  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*f% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*j% h %m ."MFBPCR"\AS.seek 0.82ml F*U'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*_  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *k'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0218h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ARV.dial :carrier F*Z  +11 h  F*o% h %V ."MFBPCR"\AS.seek 0.5ml F*x'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*t  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*[  +22 h ."MFBPCR"delay 2 seconds +200 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*d n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*8 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*} n +21 h n ."MFBPCR"delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*s  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*#  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*M% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*L % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0218h.pcr F*w% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*^ % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F**  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*7  +32 h  ."MFBPCR"\ATV.dial :air3 F*m % +86 h % F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +21 h F*:% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*n  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0220h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*R% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*@  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +21 h ."MFBPCR"delay 2 seconds +200 F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *N'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*g n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*T  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*a' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*6  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +22 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*W  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*o % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0220h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*k @@T ."MFBPCR"\ASV.dial :SPEdelivery F*x  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*i  +151 h F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *t' +423 h ' ."MFBPCR"\ATV.dial :ASV F*[ n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*b  +43 h  F*I% h % ."MFBPCR"\AS.seek 0.62ml F*d'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*~'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *y' +423 h ' ."MFBPCR"\ATV.dial :air3 F*c% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*{% h % ."MFBPCR"\AS.seek 0.71ml F*}' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*s' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*# n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*M% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*1 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*w % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F* % h % ."MFBPCR"\AS.seek 0.579ml F*4' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*!  +22 h  ."MFBPCR"\AS.seek 0ml F*v'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b **'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*& % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*?% h % ."MFBPCR"\AS.seek 0.58ml F* ' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h % q ."MFBPCR"\AS.seek 0.605ml F*7'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*l% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*5' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*< n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*:  +21 h  ."MFBPCR"\AS.seek 0ml F*F'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*>  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *H' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*/ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *2'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*n n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*+'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*= n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*D  +43 h  F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *9' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*S I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*"  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*R  +64 h  F*A% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *\\'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*%% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *)' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*@ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*B  +43 h  F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *f': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*N  +64 h  F*T% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*6% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *j' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*a I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *_'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*U  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*p n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *K'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*e n +151 h n ."MFBPCR"\AS.seek 0ml F*V'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*`  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*E % +43 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *;' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*u% h % ."MFBPCR"\AS.seek 0.96ml F*Z'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %0 ."MFBPCR"\AS.seek 0.985ml F*X' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*C% h % ."MFBPCR"\AS.seek 0.97ml F*q'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*[ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *I'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *]'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *~' +9988 h ' ."MFBPCR"\ATV.dial AIV F*c I +43 h I ."MFBPCR"\AS.seek 0.02ml F*y' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*{'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*M'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F**'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *2'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*='V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:20:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 2:17.226612 #-> exit #m\EXIT @1534504942.93CEST @1534505044.97CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:12, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 13:24:49CEST17-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F*~% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'& ."readyMFB"\AFV.select :holdingCoil F* h & M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors ."airStroke"\CS.seek 0ml .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust C'/'4 .t\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&Z% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV P%j  .t\CBV.dial PRV C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&s& A('X h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &Z'Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 .\Forearm.seek 1 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &{'υ +21 h 'υ M&~& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C F*& @@T C'Q& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*$'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F**% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&@1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&_/ +150 h / M&Y& h &- M&j% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C';(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +30 h (W C'e& h &W .tHand.open M&x1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'[% h %4 .t\CSV.dial CSR C']  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'i'p +245 h 'R ."MFBPCR"\ATV.dial :air2 F*C  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *~' +1105 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'y% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'# @ +76 h @ .t\CS.seek 0ml C's'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'm'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'5  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +563 h ' ."MFBPCR"delay 5 seconds +137 .t\CS.seek 3ml C'$'0 +363 ."MFBPCR"\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F**' +80 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'.% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'l% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C':& h &W C'<% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'E'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'D% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'R'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'S& h &W C'=% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  '%  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C'\\'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'Q& h &W C'N% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'L% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV C'g  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'1  +76 h  .t\CS.seek 0ml C'U'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&p& h &ӷ M&Z% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&x1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'[& h & .tHand.open M&I1 +2 h 1 M&]& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +7 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&u/ +150 h / M&v& h &- M&i% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'r& h &W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'\ % h %4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '}'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +505 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F* % h % ."MFBPCR"\AS.seek 0.52ml F*' +939 h '4 #t\Purging external Intake C'& h &W C'z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# '  +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'('4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C's  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'0% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +85 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteR F*/  +21 h  ."MFBPCR"\AS.seek 0.3ml F*l': +29 .t\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'n'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'J( +416 h ( M&9& h &ӷ M&D% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&S1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [coreNEG] begun 01:00:31 #\Extending 18tun until completion of MFBPCR +1978 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*R'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +86 h F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *%'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*Q'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*a  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *\\% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *T' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *L% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *f' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*g% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *1' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*@% h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Y n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*V % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *o'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*; I +21 h I F*p% h %v ."MFBPCR"\AS.seek 0.059ml F*Z'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*W % +21 h % F*)% h %H ."MFBPCR"\AS.seek 0.069ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h % ."MFBPCR"\AS.seek 0.769ml F*k' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h % q ."MFBPCR"\AS.seek 0.794ml F*I' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*C'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*e I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +128 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*y% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*w' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F* ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*z% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*M % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.23ml F*s' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *5'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*+'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1031 h '\  F*.% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*/% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*7% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *A' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3472 h 'V ."MFBPCR"\SPE.coast F*>  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*R  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 F*1  +64 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi !; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*o  +21 h ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +65 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +86 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0104h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*$' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*0  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +21 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0104h.pcr F*<% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*6  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +21 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*%  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0107h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +11 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*^  +22 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*r% h %Fj ."MFBPCR"\AS.seek 0.524ml F*8' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*\ ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*U  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*}'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.6ml F**'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*M  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0107h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*(  +21 h ."MFBPCR"\ATV.dial :air3 F*s % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *''Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*$% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F*7 % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*<'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*S  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*N  +10 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*g  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +22 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*L I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*X' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*` n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*;' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*j  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.536ml F*W'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +107 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*)'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +21 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*Z'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*K  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*q  +22 h ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*8  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*c  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*$  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*3 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*t I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*: % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.6ml F*G'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *]' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +21 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *C'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0114h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*w  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*} n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*M  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*5' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*$ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0114h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*0  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*.  +151 h F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*F  +43 h  F*7% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*3 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *>'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*9 % +43 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +423 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +21 h F*S% h % ."MFBPCR"\AS.seek 0.71ml F*6' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"User Abort!! h % H ."MFBPCR"\AS.seek 0.735ml F*2' Ű #t-> ls !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message ls !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message exit ."MFBPCR"\SPE.coast .t\AS.stop #m\EXIT @1534505204.35CEST @1534505291.77CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refix ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:14:30CET01-Jan-70 .delayUntil "16:14:30.00" +5486999 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:28:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 16:14 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:28:35, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 16:14" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:28:39, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 2 at 13:28:43CEST17-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:28:39, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*~% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&% F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors C'/'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&Z% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&s& A('X h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &Z'Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&{'υ +21 h 'υ M&~& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& F*& @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*`  P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV C'G  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 2 into Collection Clamp C'L& h & M&@% h %\  A(g% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (1( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h &  M&^& h &- M&_% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +166 h ( M&j& h & A(4& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(p& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(Z' +77 h ' A(K& h &  M&;& h & ."WCR"\Elbow.seek 2 M&d( +6 h ( M&u& h & A(e& h &  ."WCR"\Forearm.seek 2 + 300 counts M&x'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &k/ +150 h / ."downElevator"\SE.seek :down M&q& A(o'X h & M&[% h %; ."WCR"\Forearm.seek :retract M&]'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' ."WCR"\CC.seek :closed C'v(W +3 ."MFBPCR"\ATV.dial :waste2 F*t % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&b1 +150 h 1 M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +29 h '\  ."WCR"\SC.coast #collectSample 0ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +6 h + S)8% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X': +25 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'9  'E  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'D'4 +1919 h '4 ."WCR"delay 2:00 +2351 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*H'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*6 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *G'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*!  +86 h F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *S'N +440 ."WCR"\CBV.dial :waste2b C'=  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'P' +41 h ' ."WCR"delay 1:00 +528 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F* n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*"  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *%% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *B' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +79 ."WCR"\CTV.dial :air,avoiding: :intake C'\\  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Q  +38 h  C'N% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'a' +26 ."MFBPCR"\AFV.select :holdingCoil F* +7 *f% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *T' +573 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'@ @ +75 h @ +50 S)g% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'U% h % ."WCR"\CSV.dial between air and CTV C'^ ` +57 h ` ."WCR"\CS.seek 7.288ml C'_' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'`  'j  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"delay 2:00 +1030 h ' ."MFBPCR"delay 5 seconds +500 F*p% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *W' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*K% h % ."MFBPCR"\AS.seek 0.279ml F*;'Q +933 ."WCR"\CBV.dial :waste2b C'u  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'e' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'V  +150 h C'k& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&q% h %\  A([% h %X A(Y. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (X( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (i& h & M&C& h &ӷ M&x% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &Z( +142 h ( M&t& h & A(o& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +89 h ' A(]& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A()'K +254 h 'K M&I& h & A(b& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &{'Y +25 h 'Y ."WCR"\Elbow.hold M&c, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(8- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&% h %Y A(~& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'υ +21 h 'υ M&1& h & A(r& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M& & h & M&w% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(}& h & A(& h & M&& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &s( +142 h ( M&& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &''Y +93 h 'Y ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&?% h %Y A(O& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(,& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(m'X +100 (% h %B M&5% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'$& h & .tHand.open M&1 + A(6 + h 1 M&z& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&*(- +166 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&7'η +91 h 'η .tHand.close M&0/ +150 h / M&/& h &- M&n% h %η .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &A(ӷ +264 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C':(W +416 h (W C'<& h &W .tHand.open M&E1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'J& h &W C'>% h % .t\CSV.dial CSR C'6  +37 h  C'H% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'!'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'R% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'G'[ +463 h '[ .t\CSV.dial :waste C'= +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +385 h 'Q ."MFBPCR"delay 30 seconds +265 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C'%% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'\\  .t\CTV.dial CSV C'Q  +38 h  +113  .t\CBV.dial :waste1 C'N @ +75 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'P& h &W C'B% h %4 .t\CSV.dial :flush C'a  +75 h  .t\CS.seek 5ml C'@'p +650 h 'p .tdelay 5 seconds +64 ."MFBPCR"\ATV.dial :waste,via: :air3 F*g n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +286 .t\CSV.dial :air C'U @ +38 h @ C'^% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +38 h  .t\CBV.dial :waste2b C'`  +75 h  .t\CS.seek 0ml C'_'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'p  +16 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*T % +21 h  C'K% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'W'P +15 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *4'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*V I +21 h I F*k% h %v ."MFBPCR"\AS.seek 0.059ml F*q'H +455 h 'P .tdelay 5 seconds +171 h 'H ."MFBPCR"\ATV.dial :air3 F*[ % +21 h % F*Y% h %H ."MFBPCR"\AS.seek 0.069ml F*i' +222 h ' ."MFBPCR"delay 2 seconds +86 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +108 ."MFBPCR"\ATV.dial :bleach F*x  +22 h F*t% h % ."MFBPCR"\AS.seek 0.769ml F*o' q +580 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  +37 h  .t\CBV.dial :waste1 C'h @ +76 h @ .t\CS.seek 0ml C'y'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'I  +38 h  C'b% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '8'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'~  +38 h  .t\CBV.dial :waste2b C'1  +75 h  .t\CS.seek 0ml C'r'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'd'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV C') .t\CBV.dial CSV C'{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'M'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'\ '4 +507 h '4 #t\Flushing upper manifold C'c& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +32 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +152 h '4 C'?& h &W C'O% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C','0 +110 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*#' k +396 h '0 .tdelay 5 seconds +129 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*5'  +49 .t\CSV.dial :air C'$ @ +38 h @ C'% h %0 .t\CS.seek 5ml C'z'p +237 h '  ."MFBPCR"delay 3 seconds +179 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +121 ."MFBPCR"\ATV.dial :AIV F*' I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +421 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'.'4 +507 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'm% h %4 .t\CSV.dial :flush C'X  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'/% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'7  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'2'4 +377 h ' ."MFBPCR"\ATV.dial :waste F** n +128 h n ."MFBPCR"\AS.seek :empty F*3'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*<% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *E' +102 h '4 #t\Flushing lower manifold's waste2b port C'9& h &W C'J% h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C'6'p +44 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*l% h % ."MFBPCR"\AS.seek 0.579ml F*e' +384 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'!% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CTV C'G  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C'Q( +416 h ( M&N& h &ӷ M&% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&;1 +150 h 1 M&U% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'@& h & .tHand.open M&j1 +2 h 1 M&`& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &L(- +7 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&T'η +91 h 'η .tHand.close M&u/ +150 h / M&_& h &- M& % h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +264 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'q(W +416 h (W C'x& h &W .tHand.open M&t1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C'h% h %4 .t\CSV.dial CSR C'C  +37 h  C'I% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'b'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '8'[ +463 h '[ .t\CSV.dial :waste C'~ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +2009 h '4 #t\Purging external Intake C'& h &W C' % h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'd '{  +37 h +38 .t\CTV.dial :intake C')  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +415 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +172 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'M  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'c  +46 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +29 h  .t\CS.seek 5ml C'\ 'p +496 h '\ 48 ."MFBPCR"delay 3 seconds +155 h 'p .tdelay 5 seconds +145 ."MFBPCR"\ATV.dial :bleach F*}  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*v' +324 h '  ."MFBPCR"delay 3 seconds +9 .t\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +254 ."MFBPCR"\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +34 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'? +113 h .t\CTV.dial :air .\CS.seek 0ml C'O'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'o& h &W C's% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'$'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'#% h %0 .t\CS.seek 5ml C','p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +506 h '0 .t\CSV.dial :waste C'5 +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&X' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C'0( +416 h ( M&/& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &7(- +264 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&1 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Completed [coreNEG] begun 16:15:01 #\Extending 18tun until completion of MFBPCR +3920 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*> % +107 h % F*3% h %V ."MFBPCR"\AS.seek 0.59ml F*H' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*l n +107 h n ."MFBPCR"\ASV.dial :wasteR F*2% h %  ."MFBPCR"\AS.seek 0.09ml F*E' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*! I +86 h I F*6% h % ."MFBPCR"\AS.seek 0.12ml F*G' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +22 h % F*=% h % ."MFBPCR"\AS.seek 0.13ml F*S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*%% h % ."MFBPCR"\AS.seek 0.23ml F*\\' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h % ."MFBPCR"\AS.seek 0.255ml F*:'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*a n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *U'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*P @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*j I +86 h I ."MFBPCR"\AS.seek 0ml F*`'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*p'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*K  +43 h  #"MFBPCR"\Swish #1 of 2 F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *u'\  +1031 h '\  F*_% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*B% h %(A ."MFBPCR"\AS.seek 0.6ml F*^'\  +1032 h '\  F*T% h %\  ."MFBPCR"\AS.seek 0.5ml F*4'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*[% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *L' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*V % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +3472 h 'V ."MFBPCR"\SPE.coast F*x  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0117h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*W  +10 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*1  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*d' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*) % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*e'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refix ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0117h.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*O  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*5'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*X  +32 h  ."MFBPCR"\ATV.dial :air3 F*F % +86 h % F*n% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0120h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*A  +10 h  F*+% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *D'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*U I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*j% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*` % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*g' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*_% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*B  +21 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.65ml F*x' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0120h.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*\ % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*?% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0122h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*.% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b */'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*n  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*<  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*>' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.536ml F*l'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.6ml F*6'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*E  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*G  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*S% h % \\ ."MFBPCR"\AS.seek 0.326ml F*A'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET84553.48 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0122h.pcr F*\\% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*N  +21 h ."MFBPCR"\ATV.dial :air3 F*: % +87 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*`'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*@% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*_  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*g  +65 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*4  +32 h  ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +21 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0200h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ARV.dial :carrier F*d  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*c'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*?% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*v n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*&' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*o n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.65ml F*'' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0200h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*q  +22 h ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*6 % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*=% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0203h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*u'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*4  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +22 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*x n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*P I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*I' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*y % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +107 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*{  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*M% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Z'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0203h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\ % h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F*# % +86 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*i% h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0205h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +21 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*S n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*=% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*%  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*+' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*N  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*a n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0205h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*g @@T ."MFBPCR"\ASV.dial :SPEdelivery F*u  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +151 h F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *4' +423 h ' ."MFBPCR"\ATV.dial :ASV F*t n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*W  +43 h  F*V% h % ."MFBPCR"\AS.seek 0.62ml F*x'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*h n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *P'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*] % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *b' +423 h ' ."MFBPCR"\ATV.dial :air3 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*Q% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*{' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*1% h % Ű ."MFBPCR"\AS.seek 0.72ml F*r' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*M% h % zG ."MFBPCR"\AS.seek 0.365ml F*)' +7161 h ' ."MFBPCR"\ATV.dial :waste F*d n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*Z% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.579ml F*c' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*e' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*v n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*& % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h % ."MFBPCR"\AS.seek 0.58ml F*o' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*,'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +21 h F*$% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*'  +21 h  ."MFBPCR"\AS.seek 0ml F*z'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *q' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*i n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *5'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*k n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +202 h 'R ."MFBPCR"\SPE.coast F*n  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*F n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*<  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *X' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b **': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*J% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *2'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*R n +65 h n F*l% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *!' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *6'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*H  +43 h  F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *=': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*E  +64 h  F*%% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *\\'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*" n +65 h n F*N% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *:' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*f  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *A': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*a n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*; n +151 h n ."MFBPCR"\AS.seek 0ml F*D'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*U  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*j % +43 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*K% h % ."MFBPCR"\AS.seek 0.96ml F*p'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %0 ."MFBPCR"\AS.seek 0.985ml F*B' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*T% h % ."MFBPCR"\AS.seek 0.97ml F*['c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*t I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *V'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*P  +43 h  F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *C' +9988 h ' ."MFBPCR"\ATV.dial AIV F*I I +43 h I ."MFBPCR"\AS.seek 0.02ml F*b' +9880 h ' ."MFBPCR"\ATV.dial :waste F*~ n +129 h n ."MFBPCR"\AS.seek 0ml F*Q'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*Y% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*M'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*#  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *5'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*/ % +108 h % ."MFBPCR"\AS.seek 0ml F*F'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:29:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 2:16.399816 #-> exit #m\EXIT @1534505528.66CEST @1534841337.20CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 08:38:50CET02-Jan-70 .delayUntil "08:38:50.00CET02-Jan-70" @CET113930.00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% h %\  M&?& h &_ A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> InitialPurge !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"(uninitialized constant InitialPurge: name:InitialPurge #-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&E1 +150 h 1 M&F& h &_ M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &H(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &K'η +91 h 'η .tHand.close M&L/ +150 h / M&M& h &- M&N% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'U& h &W .tHand.open M&V1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'Z% h %4 .t\CSV.dial CSR C'[  +151 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '^% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +462 h '[ .t\CSV.dial :waste C'` +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +2010 h '4 #t\Purging external Intake C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f 'g  h +75 .t\CTV.dial :intake C'h  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'i'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'j  +75 h #t\Flushing upper manifold C'k& h &W C'l% h %4 .t\CSV.dial :flush C'm  +76 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'p% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'w& h &W C'x% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&\ & h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t-> exit #m\EXIT @1534842621.68CEST @1534842663.64CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 08:42:11CET02-Jan-70 .delayUntil "08:42:11.00CET02-Jan-70" @CET114131.00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:11:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 08:42 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:11:54, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 08:42" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:11:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'Q .t\CBV.dial CSV C'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:12:03CEST21-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:11:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&I& h &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(E'Z .t\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&&& A(A'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %Y A(p& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& h &W F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*t  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  'H  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&x% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0211h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'q  ."WCR"\CSV.dial CTV C':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &M'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&H% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'q% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'}  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C'? @ +75 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'c  +38 h  C'{% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'Y'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '$'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'/  +151 h .t\CS.seek 0ml C'1'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +37 h .t\CS.seek 0ml C'('4 +507 h '4 #t\Flushing upper manifold C'3& h &W C'8% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'>% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'0 +38 h .t\CS.seek 0ml C''4 +506 h '4 C''& h &W C',% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'2% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'A'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'I'4 +507 h '4 #t\Flushing lower manifold's waste1 port C')& h &W C'% h %4 .t\CSV.dial :flush C'R  +37 h  .t\CS.seek 5ml C'B'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +37 h @ C'%% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  'N  +38 h  +113  .t\CBV.dial :waste1 C'\ @ +38 h @ .t\CS.seek 0ml C'L'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'H& h &W C'S% h %4 .t\CSV.dial :flush C']  +76 h  .t\CS.seek 5ml C'_'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'% h %p .t\CS.seek 7ml C'M'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'^'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&Z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'P( +416 h ( M&W& h &ӷ M&\\% h % .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &e(- +264 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&.1 +150 h 1 M&"% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&q1 +2 h 1 M&h& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&C'η +91 h 'η .tHand.close M&d/ +150 h / M&T& h &- M&% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'|(W +416 h (W C'n& h &W .tHand.open M& 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +37 h  C'j% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'y'4 +2009 h '4 #t\Purging external Intake C'x& h &W C';% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w '  +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'?'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'c& h &W C'{% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +37 h @ C'% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C'$ +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'/% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'1'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'7% h %0 .t\CS.seek 5ml C'3'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'8'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +507 h '0 .t\CSV.dial :waste C'( +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&>& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&9' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'<( +416 h ( M&'& h &ӷ M&,% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 08:42:42 #\Extending 18tun1 until completion of MFBPCR +256961 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0211h25ml.pcr F*J% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*B% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*F'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*l  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*f'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*:  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0214h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*6'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*a  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*t I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*}'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*y  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*c  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0214h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Y  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*8'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +65 h  F*0% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *`'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*2  +31 h  ."MFBPCR"\ATV.dial :air3 F*G % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*I% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0216h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*V  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +22 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*\\  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*m  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.53ml F*.' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*"  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*:'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +108 h  ."MFBPCR"delay 7 seconds +700 F*q% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +21 h n ."MFBPCR"delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.6ml F**'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*e  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*D  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0216h25ml.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*P  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.51ml F*a'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*x'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*y% h %q ."MFBPCR"\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 0.47ml F*'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0218h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*0  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +10 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *,'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*G  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*= I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*J n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*R  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*H% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*F  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*L  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F*]'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0218h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +21 h F*+% h %Z ."MFBPCR"\AS.seek 0.81ml F*Q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*f% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*.% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 1ml F*5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*T  +32 h  ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*n  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0221h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ARV.dial :carrier F*x  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *{'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*s I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*~' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Y % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +107 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*4 n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.65ml F*(' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*0'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*` % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0221h25ml.pcr F*!% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *%'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*S% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*]'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*M% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0223h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*.  +43 h  ."MFBPCR"\ARV.dial :carrier F*g  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*T  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F**  +21 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*D' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*C  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*P  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*p'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*n  +22 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.6ml F*^'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*t% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*;% h % \\ ."MFBPCR"\AS.seek 0.326ml F*y'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET174832.94 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0223h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*}  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :ASV F*? n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*v  +43 h  F*c% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *s'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*~% h % ."MFBPCR"\AS.seek 0.53ml F*$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*4' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*3' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +21 h F*1% h % Ű ."MFBPCR"\AS.seek 0.72ml F*&' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*8 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*k% h % zG ."MFBPCR"\AS.seek 0.365ml F*(' +7161 h ' ."MFBPCR"\ATV.dial :waste F*> n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *`'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*! % +43 h % F*0% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*,% h % ."MFBPCR"\AS.seek 0.579ml F*K' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*2'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*A% h %V ."MFBPCR"\AS.seek 0.01ml F*%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*U' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +21 h % ."MFBPCR"delay 3 seconds +300 F*B% h % q ."MFBPCR"\AS.seek 0.605ml F*H'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F*F% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*l n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*S  +21 h  ."MFBPCR"\AS.seek 0ml F*L'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*_  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *Z' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*M n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *]'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*@  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*+'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*V n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*\\  +43 h  F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *Q' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*m I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*"  +42 h  F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *g': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*i  +64 h  F*[% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *q'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*5 n +65 h n F*:% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *b' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*T I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b **'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*h  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*d  +64 h  F*C% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *6'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*D n +65 h n F*P% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*u  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *p': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *a'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*j'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*t  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*^ % +43 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*x'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %0 ."MFBPCR"\AS.seek 0.985ml F*y' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*w% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*? I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *c'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *{'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*~ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*$% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F*0% h %n ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +64 h  F*'% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *G'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*9 % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*%'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*B% h %m ."MFBPCR"\AS.seek 0.82ml F*U'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *F'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Z'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *]'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*V'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:12:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:20.873198 #-> exit #m\EXIT @1534842863.34CEST @1535276973.17CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi$; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:46:42CET03-Jan-70 .delayUntil "02:46:42.00CET03-Jan-70" @CET179202.00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:49:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 02:46 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:50:15, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 02:46" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:50:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R 'Q  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 11:50:23CEST26-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:50:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % C'/'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'Q9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'R9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Q& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&R(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&[% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&s& h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(Q. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &['Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(R& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &{'υ +21 h 'υ M&~& h & A(Z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%Q  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'R& ."MFBPCR"\ASV.dial :SPEdelivery F*& @@T h &W F*`  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *['6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'/  '7  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'Q& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&@1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&_/ +150 h / M&Y& h &- M&j% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C';(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +30 h (W C'e& h &W .tHand.open M&x1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'Z% h %4 .t\CSV.dial CSR C']  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'i'p +245 h 'R ."MFBPCR"\ATV.dial :air2 F*C  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *~' +1105 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'y% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'# @ +76 h @ .t\CS.seek 0ml C's'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'm'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'5  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +563 h ' ."MFBPCR"delay 5 seconds +137 .t\CS.seek 3ml C'*'0 +363 ."MFBPCR"\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*$' +80 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'.% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'l% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C':& h &W C'<% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'E'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'D% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'Q'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'S& h &W C'=% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV C'%  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C'\\'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'R& h &W C'N% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'L% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'g  +37 h  .t\CBV.dial :waste2b C'1  +76 h  .t\CS.seek 0ml C'U'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&p& h &ӷ M&[% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&x1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&I1 +2 h 1 M&]& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +7 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&u/ +150 h / M&v& h &- M&i% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'r& h &W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'\ % h %4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '}'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +505 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F* % h % ."MFBPCR"\AS.seek 0.52ml F*' +939 h '4 #t\Purging external Intake C'& h &W C'z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# '  +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'('4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C's  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV C', .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'0% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +85 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteR F*7  +21 h  ."MFBPCR"\AS.seek 0.3ml F*l': +29 .t\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'n'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'J( +416 h ( M&9& h &ӷ M&D% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&S1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [coreNEG] begun 02:47:13 #\Extending 18tun until completion of MFBPCR +1978 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*Q'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +86 h F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *%'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*R'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*a  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *\\% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *T' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *L% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *f' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*g% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *1' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*@% h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Y n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*V % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *o'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*; I +21 h I F*p% h %v ."MFBPCR"\AS.seek 0.059ml F*['H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*W % +21 h % F*)% h %H ."MFBPCR"\AS.seek 0.069ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h % ."MFBPCR"\AS.seek 0.769ml F*k' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h % q ."MFBPCR"\AS.seek 0.794ml F*I' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*C'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*e I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +128 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*y% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*w' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F* ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*z% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*M % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.23ml F*s' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *5'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*+'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F**  +43 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1031 h '\  F*.% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*7% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*/% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *A' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3472 h 'V ."MFBPCR"\SPE.coast F*>  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0304h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*Q  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 F*1  +64 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi$; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0304h.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*o  +21 h ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +65 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +86 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0306h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F**' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*0  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +21 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*/'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0306h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*< @@T ."MFBPCR"\ASV.dial :SPEdelivery F*D  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*2  +150 h F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *=' +424 h ' ."MFBPCR"\ATV.dial :ASV F*! n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*G  +43 h  F*l% h % ."MFBPCR"\AS.seek 0.62ml F*E'\ +2034 h '\  ."MFBPCR"\ATV.dial :waste F*Q n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*B % +43 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *%' +424 h ' ."MFBPCR"\ATV.dial :air3 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*R% h % ."MFBPCR"\AS.seek 0.71ml F*a' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h % H ."MFBPCR"\AS.seek 0.735ml F*L' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*1% h % Ű ."MFBPCR"\AS.seek 0.72ml F*Y' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*` n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*f% h % zG ."MFBPCR"\AS.seek 0.365ml F*X' +7161 h ' ."MFBPCR"\ATV.dial :waste F*j n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*; % +42 h % F*V% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *o' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*)% h % ."MFBPCR"\AS.seek 0.579ml F*p' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %  ."MFBPCR"\AS.seek 0.604ml F*['\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Z% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*d' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*] n +86 h n ."MFBPCR"\ASV.dial :wasteR F*K  +21 h  ."MFBPCR"\AS.seek 0ml F*k'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *C'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*v % +108 h % F*I% h %V ."MFBPCR"\AS.seek 0.01ml F*4' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*u% h % ."MFBPCR"\AS.seek 0.58ml F*i' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*h% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*O' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*r  +21 h  ."MFBPCR"\AS.seek 0ml F*8'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *c' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b * '^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*U n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +201 h 'R ."MFBPCR"\SPE.coast F*}  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*w n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*$  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *z' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *#': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*( n +64 h n F*s% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*?  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *5': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *+'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*0% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F** I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *~'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*7  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *.': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*3'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*F  +85 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*/ % +43 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*A% h % ."MFBPCR"\AS.seek 0.96ml F*:'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*D' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*2% h % ."MFBPCR"\AS.seek 0.97ml F*S'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*6 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *G'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *='R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *"' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*N' +9880 h ' ."MFBPCR"\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.seek 0ml F*\\'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*R% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*g % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *@'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*L% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*&  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*W  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *o'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*]  +32 h  ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*u'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*{% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*U n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *}'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2548 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 2, 2 => 20, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:50:26, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 1:36.240804 #-> exit #m\EXIT @1535277120.40CEST @1535305156.03CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi(; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 09:56:12CET03-Jan-70 .delayUntil "09:56:12.00CET03-Jan-70" @CET204972.00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:39:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 09:56 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:39:40, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 09:56" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:39:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 19:39:48CEST26-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:39:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% h % ."airStroke"\CS.seek 0ml E ;3\\0\\0 #t\Calibrating pressure and temperature sensors C'/'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&[% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(s'X h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&41 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &['Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &{'υ +21 h 'υ M&~& h & A(Z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%4 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& F*& @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *['6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*s' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&@1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&_/ +150 h / M&Y& h &- M&j% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C';(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +30 h (W C'e& h &W .tHand.open M&x1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'Z% h %4 .t\CSV.dial CSR C']  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'i'p +245 h 'R ."MFBPCR"\ATV.dial :air2 F*C  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *~' +1105 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'y% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'# @ +76 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'm'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'4 's  +38 h +75 .t\CTV.dial :air C'5  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +563 h ' ."MFBPCR"delay 5 seconds +137 .t\CS.seek 3ml C'*'0 +363 ."MFBPCR"\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*$' +80 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'.% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'l% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C':& h &W C'<% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'E'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'D% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'R'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'S& h &W C'=% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'%  '"  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C'\\'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'Q& h &W C'N% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'L% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV C'g  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'1  +76 h  .t\CS.seek 0ml C'U'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&p& h &ӷ M&[% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&x1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&I1 +2 h 1 M&]& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +7 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&u/ +150 h / M&v& h &- M&i% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'r& h &W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'\ % h %4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '}'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +505 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F* % h % ."MFBPCR"\AS.seek 0.52ml F*' +939 h '4 #t\Purging external Intake C'& h &W C'z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'#  ' +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'('4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'0% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +85 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteR F*/  +21 h  ."MFBPCR"\AS.seek 0.3ml F*l': +29 .t\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'n'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'J( +416 h ( M&9& h &ӷ M&D% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&S1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [coreNEG] begun 09:56:43 #\Extending 18tun until completion of MFBPCR +1978 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*R'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +86 h F*%% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *"'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*Q'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*a  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *\\% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *T' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *L% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *f' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*g% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *1' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*@% h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Y n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*V % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *o'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*; I +21 h I F*p% h %v ."MFBPCR"\AS.seek 0.059ml F*['H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*W % +21 h % F*)% h %H ."MFBPCR"\AS.seek 0.069ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h % ."MFBPCR"\AS.seek 0.769ml F*k' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h % q ."MFBPCR"\AS.seek 0.794ml F*I' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*C'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*e I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +128 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*y% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*w' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F* ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*z% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*M % +22 h % F*#% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *5'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*4 @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*+'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F**  +43 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1031 h '\  F*.% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*/% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*7% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *A' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3472 h 'V ."MFBPCR"\SPE.coast F*>  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0311h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*R  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*%' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 F*1  +64 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi(; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0311h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*; @@T ."MFBPCR"\ASV.dial :SPEdelivery F*p  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*W  +151 h F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *[' +423 h ' ."MFBPCR"\ATV.dial :ASV F*_ n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*x  +43 h  F*d% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*Z n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *]'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*K'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*I % +43 h % F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *C' +424 h ' ."MFBPCR"\ATV.dial :air3 F*v% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*u% h % ."MFBPCR"\AS.seek 0.71ml F*i' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h % H ."MFBPCR"\AS.seek 0.735ml F*O' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*^% h % Ű ."MFBPCR"\AS.seek 0.72ml F*h' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*8 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*r' +7161 h ' ."MFBPCR"\ATV.dial :waste F*\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*U' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*w'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*M  +22 h  ."MFBPCR"\AS.seek 0ml F*#'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*(' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*?% h % q ."MFBPCR"\AS.seek 0.605ml F*''\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*s' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*m n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*5  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F**  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *0' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *.'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*~ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*7  +42 h  F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *F' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +42 h  F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *:': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*9  +64 h  F*<% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *D'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*6 n +65 h n F*2% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *S' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*= I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*l  +43 h  F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*B n +65 h n F*%% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*N I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*\\  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*a n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *T'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*g n +150 h n ."MFBPCR"\AS.seek 0ml F*L'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*@  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*1 % +43 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*X% h % ."MFBPCR"\AS.seek 0.96ml F*j'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %0 ."MFBPCR"\AS.seek 0.985ml F*V' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*)% h % ."MFBPCR"\AS.seek 0.97ml F*o'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*_ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Z'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *k'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*]  +43 h  F*I% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *K' +9988 h ' ."MFBPCR"\ATV.dial AIV F*v I +43 h I ."MFBPCR"\AS.seek 0.02ml F*C' +9880 h ' ."MFBPCR"\ATV.dial :waste F*q n +129 h n ."MFBPCR"\AS.seek 0ml F*u'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*y  +21 h ."MFBPCR"\ATV.dial :air3 F*i % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*M  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*,  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *.'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*/ % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 2, 2 => 20, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:39:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 1:29.205502 #-> exit #m\EXIT @1535305289.66CEST @1535473366.52CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:22:54, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:23:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:23:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 18:23:15CEST28-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:23:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% h % E ;3\\0\\0 ."airStroke"\CS.seek 0ml #t\Calibrating pressure and temperature sensors C'/'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&[% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%m  C'j  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(s'X M&& h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &['Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 .\Forearm.seek 1 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&m/ +150 h / .t\CC.seek :open C'j( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&{'υ +21 h 'υ M&~& h & A(Z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*& @@T .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *['6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*j I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*s' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'/  '7  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&@1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&_/ +150 h / M&Y& h &- M&m% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C';(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +30 h (W C'e& h &W .tHand.open M&x1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'Z% h %4 .t\CSV.dial CSR C']  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'i'p +245 h 'R ."MFBPCR"\ATV.dial :air2 F*C  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *~' +1105 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'y% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'# @ +76 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'j'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C's  +38 h +75 .t\CTV.dial :air C'5  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +563 h ' ."MFBPCR"delay 5 seconds +137 .t\CS.seek 3ml C'*'0 +363 ."MFBPCR"\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*$' +80 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'.% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'l% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C':& h &W C'<% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'E'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'D% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'R'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'S& h &W C'=% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  '%  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C'\\'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'Q& h &W C'N% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'L% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'g  +37 h  .t\CBV.dial :waste2b C'1  +76 h  .t\CS.seek 0ml C'U'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&p& h &ӷ M&[% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&x1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&I1 +2 h 1 M&]& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +7 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&u/ +150 h / M&v& h &- M&i% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'r& h &W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'\ % h %4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '}'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +505 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F* % h % ."MFBPCR"\AS.seek 0.52ml F*' +939 h '4 #t\Purging external Intake C'& h &W C'z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# '  +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'('4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'0% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +85 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteR F*7  +21 h  ."MFBPCR"\AS.seek 0.3ml F*l': +29 .t\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'n'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'J( +416 h ( M&9& h &ӷ M&D% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&S1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [coreNEG] begun 01:00:31 #\Extending 18tun until completion of MFBPCR +1978 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*R'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +86 h F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *%'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*Q'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*a  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *\\% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *T' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *L% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *f' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*g% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *1' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*@% h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Y n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*V % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *o'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*; I +21 h I F*p% h %v ."MFBPCR"\AS.seek 0.059ml F*['H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*W % +21 h % F*)% h %H ."MFBPCR"\AS.seek 0.069ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h % ."MFBPCR"\AS.seek 0.769ml F*k' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h % q ."MFBPCR"\AS.seek 0.794ml F*I' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*C'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*e I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +128 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*y% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*w' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F* ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*z% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*M % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *5'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*+'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F**  +43 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1031 h '\  F*.% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*7% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*/% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *A' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3472 h 'V ."MFBPCR"\SPE.coast F*>  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*R  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 F*1  +64 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*o  +21 h ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +65 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +86 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0104h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*j  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F**' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*0  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*/'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0104h.pcr F*<% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*6  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*%  +64 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +21 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0107h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +11 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*^  +21 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*r% h %Fj ."MFBPCR"\AS.seek 0.524ml F*8' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*\ ' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*U  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*}'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.6ml F*$'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*M  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0107h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*(  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *''Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +21 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*s'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +31 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*<'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*S  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0109h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*N  +10 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*g  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +22 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*L n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*X' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*` I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*;' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*m % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.536ml F*W'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*)'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +21 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*K  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0109h.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*q  +22 h ."MFBPCR"\ATV.dial :air3 F*i % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*8  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*c  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0111h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*3 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*t  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*:  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +22 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.6ml F*G'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0111h.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*m  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *]' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +21 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *C'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0114h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*w  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*} I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*M % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.65ml F*5' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*s'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0114h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*0  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*.  +151 h F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*F  +43 h  F*/% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*3 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *>'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*9 % +43 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +423 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +21 h F*S% h % ."MFBPCR"\AS.seek 0.71ml F*6' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h % H ."MFBPCR"\AS.seek 0.735ml F*2' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*G' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*l n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*R n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*B% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.579ml F*\\' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %  ."MFBPCR"\AS.seek 0.604ml F*@'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*T% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*L' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*1 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Y  +22 h  ."MFBPCR"\AS.seek 0ml F*`'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *X'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*m % +108 h % F*o% h %V ."MFBPCR"\AS.seek 0.01ml F*&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*V% h % ."MFBPCR"\AS.seek 0.58ml F*p' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h % q ."MFBPCR"\AS.seek 0.605ml F*x'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*Z% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*[' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*) n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*K  +21 h  ."MFBPCR"\AS.seek 0ml F*k'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*I  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *d' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *q'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*4 n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +202 h 'R ."MFBPCR"\SPE.coast F*i  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*^  +43 h  F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *y' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *c': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *8'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*\ n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *U' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*z  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *}': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*$% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *M'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*# n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*( n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*, n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*'  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*j % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*+% h % ."MFBPCR"\AS.seek 0.96ml F*5'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %0 ."MFBPCR"\AS.seek 0.985ml F*0' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*.% h % ."MFBPCR"\AS.seek 0.97ml F*7'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*~ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *F'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*>% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *A' +9988 h ' ."MFBPCR"\ATV.dial AIV F*t I +43 h I ."MFBPCR"\AS.seek 0.02ml F*b' +9880 h ' ."MFBPCR"\ATV.dial :waste F*: n +129 h n ."MFBPCR"\AS.seek 0ml F*<'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*S% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*D  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +43 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +21 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *i'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*q % +108 h % ."MFBPCR"\AS.seek 0ml F*u'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 2, 2 => 20, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:23:17, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 2:06.990453 !o: EOFError:bt[ "1/opt/mbari/lib/ruby/1.8/irb.rb:72:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"end of file reached !o:Errno::EIO :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error:@timestampu: Time 0Ma !o:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error #m\EXIT @1535473572.13CEST @1535569741.83CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 17:24:20CET01-Jan-70 .delayUntil "17:24:20.00" +5905999 #-> execute "decontest.rb" .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % .tdelay 3 seconds +300 F*@% h %m\  .t\AS.seek 0.335ml F*A' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*C% h % .t\AS.seek 0.32ml F*D'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*E  +43 h  .t\AS.seek 0ml F*F'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *H'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *J'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *L'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *N'V +10077 h 'V .t\ATV.dial :air2 F*O  +107 h  F*P% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *Q'(@ +5054 h '(@ .t\ATV.dial :extSrc F*R  +108 h  F*S% h %(@ .t\AS.seek 0.02ml F*T' +4851 h ' .tdelay 1 second +100 .\ATV.dial :waste F*U n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +146 h 'R .tdelay 20 seconds +2000 #deconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *W'V +31 h 'V .t\ATV.dial :air3 F*X % +43 h % F*Y% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Z' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*\\% h % .t\AS.seek 0.31ml F*]'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % .tdelay 3 seconds +300 F*_% h %m\  .t\AS.seek 0.335ml F*`' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*b% h % .t\AS.seek 0.32ml F*c'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*d  +43 h  .t\AS.seek 0ml F*e'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *g'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *i'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *k'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *m'V +10077 h 'V .t\ATV.dial :air2 F*n  +107 h  F*o% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *p'(@ +5054 h '(@ .t\ATV.dial :extSrc F*q  +107 h  F*r% h %(@ .t\AS.seek 0.02ml F*s' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*t n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +145 h 'R .tdelay 20 seconds +2000 #deconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +32 h 'V .t\ATV.dial :air3 F*w % +43 h % F*x% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*{% h % .t\AS.seek 0.31ml F*|'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % .tdelay 3 seconds +300 F*~% h %m\  .t\AS.seek 0.335ml F*' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +10077 h 'V .t\ATV.dial :air2 F*  +108 h  F*% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5053 h '(@ .t\ATV.dial :extSrc F*  +108 h  F*% h %(@ .t\AS.seek 0.02ml F*' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +145 h 'R .tdelay 20 seconds +2000 F*% h %R #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 .\ATV.dial :air3 F* % +43 h % F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*"% h %Z .t\AS.seek 0.81ml F*'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % .tdelay 3 seconds +300 F**% h %> .t\AS.seek 0.835ml F*+'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*% h %n .t\AS.seek 0.82ml F*'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*%  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*'  +64 h  F*/% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*1' +3070 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2723 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V .t\ARV.dial between carrier and air1 F*7  +32 h  .t\ATV.dial :air3 F*9 % +86 h % F*:% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *5' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*<% h % .t\AS.seek 0.81ml F*='> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % .tdelay 3 seconds +300 F*?% h %> .t\AS.seek 0.835ml F*@'m +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*B% h %m .t\AS.seek 0.82ml F*C'q +323 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*D' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +5060 h 'V .tdelay 20 seconds +2000 F*P% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 .\ATV.dial :air3 F*R % +44 h % F*S% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*T% h %Z .t\AS.seek 0.81ml F*X'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % .tdelay 3 seconds +300 F*V% h %> .t\AS.seek 0.835ml F*W'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*\\% h %n .t\AS.seek 0.82ml F*Z'q +323 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*^  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*_  +65 h  F*]% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*b' +3070 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V .t\ARV.dial between carrier and air1 F*g  +31 h  .t\ATV.dial :air3 F*h % +87 h % F*i% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *j' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*l% h % .t\AS.seek 0.81ml F*n'> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*o % +21 h % .tdelay 3 seconds +300 F*m% h %> .t\AS.seek 0.835ml F*q'm +526 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*p% h %m .t\AS.seek 0.82ml F*t'q +324 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*x' +5776 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2723 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .tdelay 20 seconds +2000 F*% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 .\ATV.dial :air3 F* % +44 h % F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z .t\AS.seek 0.81ml F*'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % .tdelay 3 seconds +300 F*\ % h %> .t\AS.seek 0.835ml F* 'n +526 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n .t\AS.seek 0.82ml F*'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .t\ARV.dial between carrier and air1 F*  +32 h  .t\ATV.dial :air3 F* % +87 h % F*"% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F**% h % .t\AS.seek 0.81ml F*'> +16109 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % .tdelay 3 seconds +300 F*% h %> .t\AS.seek 0.835ml F*+'m +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*'% h %m .t\AS.seek 0.82ml F*/'q +324 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*1' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5059 h 'V .tdelay 20 seconds +2000 F*<% h %V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0119h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ARV.dial :carrier F*;  +11 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*B n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*C  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *F'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*L' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*M % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*R  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*N'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*U I +107 h I ."MFBPCR"delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.6ml F*Q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.65ml F*X' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*^'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi !; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0119h.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*b  +21 h ."MFBPCR"\ATV.dial :air3 F*` % +87 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*g% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*m  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*r% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*~% h % ."MFBPCR"\AS.seek 0.81ml F*|'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #t-> exit #m\EXIT @1535569800.16CEST @1535619163.14CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refip; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> delayUntil '10:00 3-Aug-18' .delayUntil "10:00:00.00CEST03-Aug-18" @CEST1533283200.00 #-> delayUntil '10:00 30-Aug-18' .delayUntil "10:00:00.00CEST30-Aug-18" @CEST1535616000.00 #-> execute 18tundeploy.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"|compile error\ (ESP):3: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeploy.rb\ ^ #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:52:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 10:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:56:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 10:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:56:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q  'R h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 0ml .BACfans.suppressMail "Started mfb in tube 2 at 10:56:14CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:56:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 0ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'Q9 h 9|d +100 C'R9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b M&I& A( & h &ӷ & .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(E'Z .t\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(Q& h & M&R& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A(!% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ($& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down M&&& A(A'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'Q' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'R'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %Y A(p& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&& h & A(q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%!  +19 h  .t\PRV.dial :air P%2  +19 h  P%$% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%Q +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%R' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%!% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%2' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*I'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'Q% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'H  .t\CTV.dial CSV C'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*R n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&q% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 0ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h & A(z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &!' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M&/& h & M&$% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +90 h '\  ."WCR"\Elbow.seek CC M&s(ӷ +142 h (ӷ M&,& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ ."WCR"\Forearm.seek CC M&7' +91 h ' ."WCR"Hand.close M&(/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(4& h & A(3& h & M&0& h &ӷ M&'% h % ."WCR"\Forearm.seek :retract M&8'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &&( +142 h ( M& & h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &G'Y +93 h 'Y ."WCR"\Elbow.hold M&9, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &11 +150 h 1 M&% h %Y A(e& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&)'υ +21 h 'υ M&=& h & A(B& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(J% h % ."downElevator"\SE.seek :down A(\ 'X +100 (N% h %B M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&A1 + A(6 + h 1 M&*& h & M&%% h %\  .t\Elbow.seek FlushPuck::Garage M&I(- +166 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&H'η +91 h 'η .tHand.close M&Q/ +150 h / M&M& h &- M&% h %η .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'S(W +416 h (W C'l& h &W .tHand.open M&R1 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'_% h % .t\CSV.dial CSR C'P  +38 h  C'Z% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'E'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'i% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C'V +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '\\'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'@& h &W C'5% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '.'p +385 h 'Q ."MFBPCR"delay 30 seconds +265 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C':% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'x  'p  +38 h  +113  .t\CBV.dial :waste1 C'd @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'o& h &W C'h% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'T'p +650 h 'p .tdelay 5 seconds +64 ."MFBPCR"\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +286 .t\CSV.dial :air C'u @ +38 h @ C'|% h %p .t\CS.seek 7ml C'`'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'n'4 +766 h '4 #t\Flushing upper manifold C' & h &W C'C% h %4 .t\CSV.dial :flush C'a  +16 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*r % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *K'v +16 h  .t\CS.seek 5ml C'j'p +609 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F* % h %v ."MFBPCR"\AS.seek 0.059ml F*q'H +21 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +37 h @ C'% h %p .t\CS.seek 7ml C'w'i +68 h 'H ."MFBPCR"\ATV.dial :air3 F*} % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +105 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ '  +38 h +57 ."MFBPCR"\ATV.dial :bleach F*  +18 h .t\CTV.dial :air C'c  +4 h F*v% h % ."MFBPCR"\AS.seek 0.769ml F*Y' q +147 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'> +38 h .t\CS.seek 0ml C'z'4 +506 h '4 C'/& h &W C'$% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'?'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +37 h @ C'% h %0 .t\CS.seek 5ml C','p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'k'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'!'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'~& h &W C'(% h %4 .t\CSV.dial CSR C'2  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'4'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'3' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'0 +113 h .t\CTV.dial :intake C''  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C' ' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '7'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C's  +113 h  C'1% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +710 h ' ."connect"\CSV.dial CTV C'=  .t\CTV.dial CSV C'B  +38 h  +113  .t\CBV.dial :waste1 C'J @ +37 h @ .t\CS.seek 0ml C'8'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'&  +38 h  C'N% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +311 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*A% h % q ."MFBPCR"\AS.seek 0.794ml F**' k +183 h 'P .tdelay 5 seconds +343 h ' k ."MFBPCR"delay 3 seconds +157 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '%' +137 ."MFBPCR"\ATV.dial :bleach F*G  +21 h F*)% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +228 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'X  +34 ."MFBPCR"\ATV.dial :AIV F*U I +41 h  .t\CS.seek 0ml C'\ '4 +67 h I ."MFBPCR"\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 0.015ml F*y' +843 h '4 #tKilling upper manifold .\CSV.dial CSR C'9  +37 h  C'Q% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'M'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'H  +37 h +75 .t\CTV.dial :air C'L  +151 h .t\CS.seek 0ml C']'4 +910 h '4 .tdelay 7 seconds +24 h ' ."MFBPCR"\ATV.dial :waste F*I n +128 h n ."MFBPCR"\AS.seek :empty F*W'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +223 h ' ."MFBPCR"delay 2 seconds +166 .t\CS.seek 3ml C'^'0 +34 ."MFBPCR"\ATV.dial :bleach F*g  +21 h F*_% h % ."MFBPCR"\AS.seek 0.579ml F*P' +451 h '0 .t\CSV.dial :waste C'Z +38 h .t\CS.seek 0ml C'F'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'i% h %4 .t\CSV.dial :flush C'E  +37 h  .t\CS.seek 5ml C'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'@% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'm +113 h .t\CTV.dial :air .\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'"'0 +506 h '0 .t\CSV.dial :waste C': +38 h .t\CS.seek 0ml C'.'4 +507 h '4 C'x& h &W C'p% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.seek 3ml C'D'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +38 h @ C'h% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'u'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'|& h &W C'T% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C't'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C' % h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'r  '  +37 h  +113  .t\CBV.dial :waste1 C'a @ +38 h @ .t\CS.seek 0ml C'6'4 +452 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +14 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C' % h %4 .t\CSV.dial :flush C'K  +8 h % ."MFBPCR"delay 3 seconds +67 h  .t\CS.seek 5ml C';'p +233 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*}'\ 48 +418 h 'p .tdelay 5 seconds +107 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*j% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*q' +71 .t\CSV.dial :air C' @ +37 h @ C'{% h %p .t\CS.seek 7ml C''i +216 h '  ."MFBPCR"delay 3 seconds +200 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  +38 h  .t\CBV.dial :waste2b C'c  +62 ."MFBPCR"\ATV.dial :ASV F* n +13 h  .t\CS.seek 0ml C'w'4 +72 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*>'V +651 h '4 #tDisinfecting external Intake with kill C'& h &W C'/% h %4 .t\CSV.dial CSR C'$  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'z'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'b' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'?  ' +38 h +75 .t\CTV.dial :intake C',  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'k' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'~  +113 h  C'(% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'2'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'! +113 h .t\CS.seek 0ml C'4'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&0& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +7 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&s/ +150 h / .t\CC.seek :open C'1( +416 h ( M&7& h &ӷ M&% h % .t\Forearm.seek :retract M&='\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +265 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +91 h 'η .tHand.open M&N1 +150 h 1 M&8% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&Y1 +2 h 1 M&G& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &)(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&X/ +150 h / M&U& h &- M&%% h %η .t\Forearm.seek :retract M&'\ +9 h 'V ."MFBPCR"delay 1:00 +82 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +90 h ' .t\CC.seek :closed C'H(W +416 h (W C'& h &W .tHand.open M&L1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'l% h %4 .t\CSV.dial CSR C'y  +113 h  C'W% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'g'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '_% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ']'[ +463 h '[ .t\CSV.dial :waste C'R +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +2009 h '4 #t\Purging external Intake C'^& h &W C'% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +247 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +457 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'f .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'@  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'V'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'm  +75 h #t\Flushing upper manifold C'5& h &W C'\\% h %4 .t\CSV.dial :flush C':  +75 h  .t\CS.seek 5ml C'"'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'x @ +38 h @ C'p% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV C'. .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'o'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'h'0 +408 h ' ."MFBPCR"delay 1 second +99 h '0 .t\CSV.dial :waste C'D + ."MFBPCR"\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +30 h .t\CS.seek 0ml C''4 +70 ."MFBPCR"\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +437 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'|  +37 h  .t\CS.seek 3ml C'T'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'`'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'r +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&a& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'( +416 h ( M&6& h &ӷ M&P% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &j(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &}'η +91 h 'η .tHand.open M&;1 +150 h 1 M&v% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  #t\Completed [mbaPhase] begun 10:00:26 #\Preparing to sleep until 8AM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 8AM #\Continuing at 08:00CEST31-Aug-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:57:31, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 08:00CEST31-Aug-18" +14408 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*q n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*/' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*w% h % ."MFBPCR"\AS.seek 0.12ml F*z' +625 h ' ."MFBPCR"\ATV.dial :air3 F*? % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*,' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*k' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*!% h %X ."MFBPCR"\AS.seek 0.24ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*0 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*3  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *['5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*+ @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*7 I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*J  +43 h  #"MFBPCR"\Swish #1 of 2 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *''\  +1032 h '\  F*N% h %\  ."MFBPCR"\AS.seek 0.5ml F*8'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*=% h %(A ."MFBPCR"\AS.seek 0.6ml F*A'\  +1032 h '\  F*Y% h %\  ."MFBPCR"\AS.seek 0.5ml F*G'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b **' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*& % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +3473 h 'V ."MFBPCR"\SPE.coast F*U  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug3013h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*%  +64 h  ."MFBPCR"\ARV.dial :carrier F*B  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*_ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +107 h  ."MFBPCR"delay 7 seconds +700 F*^% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*>'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.65ml F*E' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*m  +43 h  ."MFBPCR"\ASV.dial :waste1 F*5  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refip; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118aug3013h25ml.pcr F*"% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*d  +21 h ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *h'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*T% h %n ."MFBPCR"\AS.seek 0.82ml F*`'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*r  +65 h  F*t% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*P % +86 h % F*n% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*}'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18aug3015h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*,  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +10 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*3  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*[  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*S' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*e' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.536ml F*''< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*=  +107 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*Y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*A n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*G'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*&  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18aug3015h25ml.pcr F*Q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*1  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*]  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*^  +64 h  F*R% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +32 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +87 h % F*:% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*d'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*D% h %m ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*F  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18aug3017h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ARV.dial :carrier F*a  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*9 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*n  +21 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *{'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*C I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*v' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*$ n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*w'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*z  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*b  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*~'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18aug3017h25ml.pcr F*k% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*2  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*0% h %Z ."MFBPCR"\AS.seek 0.81ml F*['> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*N  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*e  +65 h  F*=% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*G  +32 h  ."MFBPCR"\ATV.dial :air3 F*% % +86 h % F*B% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +21 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*L'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*)  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18aug3020h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ARV.dial :carrier F*4  +10 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*Z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*f n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*5  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*x n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*p% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*" I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*o % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.536ml F*d'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*F  +107 h  ."MFBPCR"delay 7 seconds +700 F*.% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +22 h n ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.65ml F*T' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*r% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18aug3020h25ml.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*9  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +87 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +31 h  ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*~'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug3022h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*8% h %V ."MFBPCR"\AS.seek 0.5ml F*''(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*G  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*%  +22 h ."MFBPCR"delay 2 seconds +200 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*U n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*+  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.53ml F*\ ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*1  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +22 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*l n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*_' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*i % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CEST1535668088.48 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218aug3022h25ml.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *5'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*:% h %Z ."MFBPCR"\AS.seek 0.81ml F*>'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*V% h %n ."MFBPCR"\AS.seek 0.82ml F*E'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*o  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*D  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *F'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*u  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +21 h F*T% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*`'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*K% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18aug3100h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*v  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*$ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*?  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*w  +21 h ."MFBPCR"delay 2 seconds +200 F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b */'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*, I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*b' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.53ml F*(' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*2  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*~'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*!  +22 h  ."MFBPCR"delay 7 seconds +700 F*3% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*0 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*[% h %\  ."MFBPCR"\AS.seek 0.65ml F*J' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*N% h % \\ ."MFBPCR"\AS.seek 0.326ml F*='{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18aug3100h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*e @@T ."MFBPCR"\ASV.dial :SPEdelivery F*'  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*A  +151 h F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *G' +423 h ' ."MFBPCR"\ATV.dial :ASV F*% n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*B  +43 h  F*&% h % ."MFBPCR"\AS.seek 0.62ml F*U'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *+'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*X% h % ."MFBPCR"\AS.seek 0.71ml F*l' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*M' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*W% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*]% h % zG ."MFBPCR"\AS.seek 0.365ml F*_' +7161 h ' ."MFBPCR"\ATV.dial :waste F*4 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*^ % +43 h % F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*@% h % ."MFBPCR"\AS.seek 0.579ml F*Z' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*:% h %  ."MFBPCR"\AS.seek 0.604ml F*5'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +21 h F*p% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*>' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*" n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +22 h  ."MFBPCR"\AS.seek 0ml F*m'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *D'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*E% h %V ."MFBPCR"\AS.seek 0.01ml F*F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*d% h % ."MFBPCR"\AS.seek 0.58ml F*h' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*u'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F* % h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*r n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*T  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *9' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *`'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +202 h 'R ."MFBPCR"\SPE.coast F*n  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*H'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*P n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*C  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *6' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +42 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *v': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*}% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*$ n +65 h n F*q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*? I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*/  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *k'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*b n +65 h n F*2% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*( I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*3  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *~': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*0 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*s n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*[  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*7 % +43 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*J% h % ."MFBPCR"\AS.seek 0.96ml F* '0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h %0 ."MFBPCR"\AS.seek 0.985ml F*=' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*Y% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*% I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *&'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b **'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *1' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Q' +9988 h ' ."MFBPCR"\ATV.dial AIV F*\ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.seek 0ml F*X'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*)% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*l % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*_'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*R% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*@  +64 h  F*f% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*F'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *`'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*I % +108 h % ."MFBPCR"\AS.seek 0ml F*P'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +1413369 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )C h  C' ' h  P% %H h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *6  h  .t\SPE.configure SPEconfig F*\ 2 *;% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:58:33, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&=1 +150 h 1 M&%& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &B(- +166 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&U/ +150 h / M&+& h &- M& % h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +264 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C')(W +416 h (W C'& h &W .tHand.open M&X1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'M  +150 h  C'y% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '4'[ +463 h '[ .t\CSV.dial :waste C'] +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '^'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'_% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'f .t\CBV.dial CSV C'i  h +75 .t\CTV.dial :intake C'\\  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C':'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Z  +75 h #t\Flushing upper manifold C'x& h &W C'p% h %4 .t\CSV.dial :flush C'5  +75 h  .t\CS.seek 5ml C'V'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +37 h @ C'm% h %p .t\CS.seek 7ml C'"'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +113 h .t\CTV.dial :air .\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +507 h '0 .t\CSV.dial :waste C'. +37 h .t\CS.seek 0ml C'd'4 +507 h '4 C'D& h &W C'|% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'F'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'h% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'u'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +507 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&K' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C'n( +416 h ( M& & h &ӷ M&I% h % .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &j(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&<1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 0ml .BACfans.suppressMail "Started mfb in tube 2 at 10:58:37CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:58:33, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 0ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'e& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&C% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(H. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (6& h &  M&;& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F*{% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *}' +160 h ( M&v& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &q' +89 h ' A(& h &  .t\SE.seek 17.71 Pucks below Top Plate A(?'* +87 h '* A(& h &  M&w& h & .t\Elbow.seek 2 M&( +6 h ( M&,& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&/'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &b/ +150 h / ."downElevator"\SE.seek :down M&z& A(2'X h & M&k% h %; .t\Forearm.seek :retract M&'\ +87 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .t\Elbow.seek CC M&s(ӷ +143 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'8(W +416 h (W .tHand.open M&'1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)7+ +6 h + S)% h % C'Y& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)m  +144 h  +6  S)V% h % .t\CBV.dial between SSV and waste2 C'O  +19 h  C'"% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'E  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '.'4 +2609 h '4 C'o% h %4 S)D% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'| @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'p9 h 9Td +100 C'\\9 h 9kd +100 C'59 h 9d +100 C':9 h 9d +100 C'm9 h 9d +100 C'V9 h 9d +100 C'O9 h 9d +100 C'"9 h 9d +100 C'E9 h 9 d +100 C'^9 h 9 d +100 C'x9 h 96d +100 C'o9 h 9Ld +100 C'D9 h 9bd +100 C'|9 h 9xd +100 C'>9 h 9d +100 C'd9 h 9d +100 C'9 h 9d +100 C'h9 h 9d +100 C'9 h 9d +100 C'F9 h 9d +100 C'u9 h 9d +100 C'T9 h 9(d +100 C' 9 h 9>d +100 C' 9 h 9Td +100 C't9 h 9id +100 C'.9 h 9d +100 C'a9 h 9d +100 C'r9 h 9d +100 C'99 h 9d +100 C'l9 h 9d +100 C')9 h 9d +100 C'I9 h 9  d +100 C'K9 h 9 d +100 C'19 h 9 *d +100 C'#9 h 9 ?d +100 C'c9 h 9 Td +100 C'<9 h 9 j d +100 C' 9 h 9 $d +100 C'9 h 9 (d +100 C'`9 h 9 ,d +100 C'9 h 9 0d +100 C'C9 h 9 4d +100 C'9 h 9 8d +100 C'H9 h 9 / +150 h / .t\CC.seek :open C'd( +416 h ( A(& h & A(h& h & M&& h &ӷ M&F% h % .t\Forearm.seek :retract M&u'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &T( +143 h ( M& & h & A( & h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +92 h 'Y .t\Elbow.hold M&., +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &a1 +150 h 1 M&r% h %Y A(9& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&l'υ +20 h 'υ M&)& h & A(I& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &K' +22 h ' A(1% h % ."downElevator"\SE.seek :down A(#'X +100 (c% h %1 M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'`' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'C { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&H1 +2 h 1 M&& h & M&6% h %\  .t\Elbow.seek FlushPuck::Garage M&-(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&e/ +150 h / M&$& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +264 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'& h &W .tHand.open M&z1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%k% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%q  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%3' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%?  C'/  h +75 .t\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%'  +37 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%Y' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%!' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%7' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.seek CC M&=' +91 h ' .tHand.close M&U/ +150 h / .t\CC.seek :open C'(( +416 h ( M&}& h &ӷ M&J% h % .t\Forearm.seek :retract M&+'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\ 'η +91 h 'η .tHand.open M&A1 +150 h 1 M&%% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'X& h & M&% h %\  A(% h %X A(*. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (y( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (0& h &  M&g& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &]( +166 h ( M&4& h & A(_& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &s' +89 h ' A(~& h &  .t\SE.seek 16.71 Pucks below Top Plate A(W'; +96 h '; A(i& h &  M&@& h & .t\Elbow.seek 2 M&p( +7 h ( M&\\& h & A(:& h &  .t\Forearm.seek 2 + 300 counts M&m'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &"/ +150 h / ."downElevator"\SE.seek :down A(E'X M&R& h & M&M% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&f(ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&V1 +150 h 1 M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'^  +151 h  .t\CSV.dial CTV C'8  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'h' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%F  +38 h  .t\PS.seek 2.5ml P% ' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%|  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%x +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%a  +151 h  .t\PS.seek :empty P%r' +2609 h ' .t\CBV.dial :waste1 C'9 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C')  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'I @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'1  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'u ` +19 h ` .t\CSV.dial :air C'T @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'<  +37 h  .t\CS.seek 0ml C''4 +2648 h '4 .t\CBV.dial :waste1 C't @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'C& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(H% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (6( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (l& h & M&O& h &ӷ M&K% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&{& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &#' +89 h ' A(& h & .t\SE.seek 2.71 Pucks below Top Plate A(.') +235 h ') M&e& h & A($& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(j' +27 h ' M&w% h %Y A(-& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'υ +21 h 'υ M&d& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &z' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(;' +36 h ' M&& h & M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +90 h '\  .t\Elbow.seek CC M&q(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'/( +416 h ( A(2& h & A(& h & M&3& h &ӷ M&'% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&n& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &Y'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &!1 +150 h 1 M&[% h %Y A(&& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&G'υ +21 h 'υ M&U& h & A(v& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &b' +22 h ' A(P% h % ."downElevator"\SE.seek :down A(}'X +100 (J% h %  M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%B  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%A' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%% +38 h .t\PRV.dial :air P%+  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%X  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%*  +18 h  P%0% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %g' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %\ ' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'4& h & .tHand.open M&_1 +2 h 1 M&~& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&i(- +166 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&S/ +150 h / M&"& h &- M&R% h %η .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +264 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &D' +91 h ' .t\CC.seek :closed C'W(W +416 h (W C'm& h &W .tHand.open M&p1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'(& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%V @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%Z  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%^  +76 h  .tdelay 2 seconds +200 P%8% h % .t\PS.seek 1.499ml P%5' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%y' +1094 h ' .t\PSV.dial :air P%`  +38 h  .t\PS.seek 0ml P%' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%h +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%F  +38 h  .tdelay 2 seconds +200 P%7% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb % 'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%|  +75 h .tdelay 2 seconds +200 P%x% h %r .t\PS.seek 0.475ml P%a' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%9'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%)  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%I  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %1' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%u +75 h .t\PTV.dial :PRV,avoiding: :puck P%T @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%<  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%t +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%C  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%H +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%l% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %O' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'K & +50 h & .t\AWV.select :waste C' +6 P%{% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %L'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %f' +1312 h ' .t\PSV.dial :air P%>  +38 h  .t\PS.seek 2.5ml P%e' +2610 h ' .t\PSV.dial :PML P%$ +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'w& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'-& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*d @@T .t\AIV.dial :bypass F*  .t\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%k% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%#' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*.% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%N' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%j' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*?  +22 h ' .t\PSV.dial PSR P%, +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*2'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%3' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*' % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*q  +21 h  F*!% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *[' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%U' +217 ."MFBPCR"\ATV.dial :AIV F*v I +43 h I F*P% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +2610 h ' .t\PSV.dial PSR P%= +113 h P%Y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%G' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +38 h  .t\PS.seek 0ml P%B' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*b n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*}% h % ."MFBPCR"\AS.seek 0.041ml F*6'% +336 h ' .t\PSV.dial PSR P%r +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%X' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%0  +37 h  .t\PS.seek 0ml P%A' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%g' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %~'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %@' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*\\ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%S  +113 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :mixloop P%R +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %Q' +5106 h ' .t\PSV.dial :air P%:  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %s' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*o  +43 h  F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *M'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*/ I +43 h I F*m% h %Q ."MFBPCR"\AS.seek 0.01ml F*p' +171 h ' .t\PSV.dial :mixloop P%E +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %(' +619 h ' ."MFBPCR"\ATV.dial :waste F*V n +128 h n ."MFBPCR"\AS.seek :empty F*D'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*Z  +86 h  F*^% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *8' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*\  +43 h F*5% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *`'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'y  +37 h  C'h% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'7' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '|  +38 h  +113  .t\CBV.dial :waste1 C'x @ +38 h @ .t\CS.seek 0ml C' '4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*a% h %_ ."MFBPCR"\AS.seek 0.434ml F*)'ݏ +110 h '4 .t\CSV.dial CSR C'I  +37 h  C'9% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'u'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*T  +22 h F*1% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*<'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*t% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'C' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'H'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&l& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&O' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'L( +416 h ( M&>& h &ӷ M&f% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &e(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 2 into Collection Clamp C'& h & M&.% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (#( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&N& h &- M&z% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &j( +166 h ( M&?& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A( & h &  M&'& h & ."WCR"\Elbow.seek 2 M&2( +7 h ( M&& h & A(q& h &  ."WCR"\Forearm.seek 2 + 300 counts M&!'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &3/ +148 h '&0 ."MFBPCR"delay 3 seconds +2 h / ."downElevator"\SE.seek :down M&&& A(n'X h & M&v% h %; ."WCR"\Forearm.seek :retract M&P'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&[& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &U' +65 ."MFBPCR"\ATV.dial :waste2 F*= % +26 h ' ."WCR"\CC.seek :closed C'Y(W +17 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +399 h (W ."WCR"Hand.open M&;1 +150 h 1 M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*b  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *}' +59 h '\  ."WCR"\SC.coast #collectSample 0ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)r% h % C'B& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'+  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *f% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *O' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +108 ."WCR"\CTV.dial :air,avoiding: :intake C'w  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'-  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +3 ."MFBPCR"\AFV.select :holdingCoil F* +3 ."WCR"\CS.seek 2ml C'' +3 F*k% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *$' +604 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'. @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'N ` +57 h ` ."WCR"\CS.seek 7.288ml C'z' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '?  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +1001 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *e' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F* % h % ."MFBPCR"\AS.seek 0.279ml F*''Q +963 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'q' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'3& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&&% h %\  A(v% h %X A(j. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (,( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ([& h & M&& h &ӷ M&!% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&=& h & A(2& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +89 h ' A(P& h & ."WCR"\SE.seek 3.71 Pucks below Top Plate A(L' +224 h ' M&c& h & A(;& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &G'Y +26 h 'Y ."WCR"\Elbow.hold M&b, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(r- h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(B' +27 h ' M&+% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&J'υ +20 h 'υ M&#& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +37 h ' M&X& h & M&0% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +89 h '\  ."WCR"\Elbow.seek CC M&%(ӷ +142 h (ӷ M&A& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ ."WCR"\Forearm.seek CC M&_' +91 h ' ."WCR"Hand.close M&}/ +150 h / ."WCR"\CC.seek :open C' ( +416 h ( A(& h & A(\\& h & M&4& h &ӷ M&S% h % ."WCR"\Forearm.seek :retract M&@'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &R( +143 h ( M&~& h & A(Q& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &o'Y +92 h 'Y ."WCR"\Elbow.hold M&i, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &"1 +150 h 1 M&% h %Y A(H& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&m'υ +21 h 'υ M&E& h & A(M& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' A(V% h % ."downElevator"\SE.seek :down A(/'X +100 (^% h % M&Z% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +90 h '\  +21 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&p1 +2 A(6 h 1 M&g& h & M&y% h %\  .t\Elbow.seek FlushPuck::Garage M&D(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.seek :garage M&|'η +91 h 'η .tHand.close M&h/ +150 h / M&& h &- M&7% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +265 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &x' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'T& h &W .tHand.open M&)1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'`& h &W C'1% h % .t\CSV.dial CSR C'u  +38 h  C'<% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'C'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'K +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 't'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C']% h %4 .t\CSV.dial :flush C'l  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'W'p +414 h 'Q ."MFBPCR"delay 30 seconds +236 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'f% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'w  .t\CTV.dial CSV C'-  +37 h  +113  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'k'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C'O% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'.'p +650 h 'p .tdelay 5 seconds +93 ."MFBPCR"\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +256 .t\CSV.dial :air C' @ +38 h @ C'N% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'$% h %4 .t\CSV.dial :flush C'  +45 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*e % +30 h  .t\CS.seek 5ml C''p +13 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*3 I +12 h 'p .tdelay 5 seconds +10 h I F*&% h %v ."MFBPCR"\AS.seek 0.059ml F*v'H +490 .t\CSV.dial :air C'j @ +38 h @ C'[% h %p .t\CS.seek 7ml C''i +97 h 'H ."MFBPCR"\ATV.dial :air3 F*! % +21 h % F*=% h %H ."MFBPCR"\AS.seek 0.069ml F*2' +222 h ' ."MFBPCR"delay 2 seconds +76 h 'i ."connect"\CSV.dial CBV C'P .t\CBV.dial CSV C'  +37 h +76 .t\CTV.dial :air C'  +11 ."MFBPCR"\ATV.dial :bleach F*c  +22 h F*;% h % ."MFBPCR"\AS.seek 0.769ml F*r' q +117 h .t\CS.seek 0ml C'+'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +507 h '0 .t\CSV.dial :waste C'# +37 h .t\CS.seek 0ml C''4 +507 h '4 C'X& h &W C'0% h %4 .t\CSV.dial :flush C'n  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'G% h %0 .t\CS.seek 5ml C'A'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'B +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'b& h &W C'}% h %4 .t\CSV.dial CSR C'*  +150 h  C'Y% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'\\' +416 h ' ."connect"\CSV.dial CBV C'4 .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'~' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Q  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '_'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'%  +113 h  C'"% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  'M  +37 h  +113  .t\CBV.dial :waste1 C'V @ +38 h @ .t\CS.seek 0ml C'@'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'R  +37 h  C'^% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Z'P +341 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h % q ."MFBPCR"\AS.seek 0.794ml F*g' k +153 h 'P .tdelay 5 seconds +372 h ' k ."MFBPCR"delay 3 seconds +128 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'y' +166 ."MFBPCR"\ATV.dial :bleach F*o  +22 h F*m% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +323 h '  ."MFBPCR"delay 3 seconds +199 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'F  +64 ."MFBPCR"\ATV.dial :AIV F*s I +12 h  .t\CS.seek 0ml C'/'4 +96 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *,' +813 h '4 #tKilling upper manifold .\CSV.dial CSR C'i  +38 h  C'h% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ '|  +38 h +75 .t\CTV.dial :air C'I  +151 h .t\CS.seek 0ml C'9'4 +909 h '4 .tdelay 7 seconds +54 h ' ."MFBPCR"\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *)' +222 h ' ."MFBPCR"delay 2 seconds +136 .t\CS.seek 3ml C''0 +64 ."MFBPCR"\ATV.dial :bleach F*`  +22 h F*1% h % ."MFBPCR"\AS.seek 0.579ml F*u' +421 h '0 .t\CSV.dial :waste C'< +38 h .t\CS.seek 0ml C'x'4 +506 h '4 #t\Flushing upper manifold C'q& h &W C'% h %4 .t\CSV.dial :flush C'C  +38 h  .t\CS.seek 5ml C'K'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l +113 h .t\CTV.dial :air .\CS.seek 0ml C't'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'>'0 +506 h '0 .t\CSV.dial :waste C'f +38 h .t\CS.seek 0ml C'W'4 +506 h '4 C'w& h &W C'-% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +37 h @ C'O% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'k'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'N'4 +507 h '4 #t\Flushing lower manifold's waste1 port C''& h &W C'.% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'$'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'e  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'z'4 +481 h '  ."MFBPCR"delay 3 seconds +285 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'3% h %4 .t\CSV.dial :flush C'&  +15 ."MFBPCR"\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +38 h  .t\CS.seek 5ml C'j'p +262 F*[% h %  ."MFBPCR"\AS.seek 0.604ml F*!'\ 48 +388 h 'p .tdelay 5 seconds +137 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*v' +41 .t\CSV.dial :air C' @ +38 h @ C'P% h %p .t\CS.seek 7ml C'c'i +245 h '  ."MFBPCR"delay 3 seconds +171 h 'i ."connect"\CSV.dial CTV C';  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'2'4 +16 ."MFBPCR"\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*+  +43 h  ."MFBPCR"\AS.seek 0ml F*#'V +621 h '4 #tDisinfecting external Intake with kill C'U& h &W C'X% h %4 .t\CSV.dial CSR C'0  +37 h  C'n% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'L' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G '  +38 h +75 .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'6' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'B  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +607 h '4 .t\CSV.dial CSR C'b  +113 h  C'}% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'*'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'Y' +1373 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&4& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &S(ӷ +6 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&%/ +150 h / .t\CC.seek :open C'"( +416 h ( M&_& h &ӷ M&% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +265 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +90 h 'η .tHand.open M&^1 +150 h 1 M&@% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'p& h & .tHand.open M&r1 +2 h 1 M&o& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &m(- +7 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&F/ +150 h / M&s& h &- M&y% h %η .t\Forearm.seek :retract M&'\ +38 h 'V ."MFBPCR"delay 1:00 +53 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +264 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'|(W +416 h (W C'\ & h &W .tHand.open M&I1 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'T% h %4 .t\CSV.dial CSR C',  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'`'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '1% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '9'[ +463 h '[ .t\CSV.dial :waste C') +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '<'4 +2010 h '4 #t\Purging external Intake C'& h &W C'q% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +276 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +426 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'K'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'l  +76 h #t\Flushing upper manifold C']& h &W C't% h %4 .t\CSV.dial :flush C'f  +75 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'-% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W +113 h .t\CTV.dial :air .\CS.seek 0ml C'{'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +437 h ' ."MFBPCR"delay 1 second +69 h '0 .t\CSV.dial :waste C' +31 ."MFBPCR"\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second h .t\CS.seek 0ml C''4 +100 ."MFBPCR"\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *k'V +406 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.seek 3ml C'.'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C'N% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'e +75 h .t\CS.seek 0ml C' '4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'[( +416 h ( M&z& h &ӷ M&u% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +91 h 'η .tHand.open M&j1 +150 h 1 M&;% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 08:00:15 #\Preparing to sleep until #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til !o:TypeError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"$Cannot convert NilClass to Time #-> exit ."MFBPCR"\SPE.coast F*  h &8 .t\AS.stop F*5 h ' k #m\EXIT !Fo:ThreadError:bt[ "7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"killed thread @1535619624.12CEST @1535619948.37CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:05:56, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:06:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:06:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:06:17CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:06:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'S9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'Q9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & M&I& h &ӷ & .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(E'Z .t\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&S& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(Q& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&A& A(&'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'S'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%Q +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %Y A(p& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&& h & A(q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&A% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&&1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Q'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*t  P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*&' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*I'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'H  .t\CTV.dial CSV C'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'Q'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*S n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&q% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*A'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'x  ':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&A& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&&& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A( & h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Q& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&S& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'x& h &W C':% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'q  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'A  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'&  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'N  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'Q +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'S& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'x% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &A(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'&& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Q  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'H .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'S% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Preparing to sleep until 8AM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 8AM #\Continuing at 08:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:07:40, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 08:00" +235134 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*A'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +107 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*Q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +444088 E ;3\\0\\0 C'Z% P%[% h %4 % M&^% S)% h %\  % M&S& h &- A(P& h & A(+% h %X C'f& h & P%i& h & E ;3\\0\\0 .tGoodNews.suppressMail "At 08:00:00 began in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:07:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&e1 + h 1 M&\\& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &m(- +6 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.seek :garage M&x'η +91 h 'η .tHand.close M&:/ +150 h / M&& h &- M&9% h %η .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'6& h &W .tHand.open M&D1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'd& h &W C'`% h %4 .t\CSV.dial CSR C'O  +151 h  C'|% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'E'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +462 h '[ .t\CSV.dial :waste C'n +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Purging external Intake C'a& h &W C'% h %4 .t\CSV.dial :flush C'u  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'j  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'y  +75 h #t\Flushing upper manifold C'& h &W C';% h %4 .t\CSV.dial :flush C'q  +76 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C'}'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w +113 h .t\CTV.dial :air .\CS.seek 0ml C'?'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'{ +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'V% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'o'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'@% h %0 .t\CS.seek 5ml C'~'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'c +75 h .t\CS.seek 0ml C'/'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +6 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&3' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C'G( +416 h ( M&(& h &ӷ M&z% h % .t\Forearm.seek :retract M&!'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &k(- +264 h (- M&>& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&'% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #tWaiting for previous MFBPCR to complete... +169809 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*A% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*<  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *&'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*B  +64 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +32 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*P  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*` I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*O% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*" n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F* ' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*E  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*K  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*;  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*w% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*?  +21 h ."MFBPCR"\ATV.dial :air3 F*{ % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*1% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *7'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*3  +32 h  ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*l n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*s I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*^ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*P  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +22 h n ."MFBPCR"delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F**  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*:  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +21 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*d'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +21 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F*|'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +31 h  ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*;% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*w% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +21 h F*{% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*}  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0113h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ARV.dial :carrier F*c  +10 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*8'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*3  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +22 h ."MFBPCR"delay 2 seconds +200 F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *z'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*$' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*!' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*<  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +22 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0113h25ml.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*F % +86 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*]% h %Z ."MFBPCR"\AS.seek 0.81ml F*M'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*_'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *P'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +86 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*C% h %m ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0115h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*|  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*p n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*j  +21 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *K'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*?  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*r'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*}  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*V'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*@  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*~' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*o  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*1'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0115h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*8  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*,  +151 h F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *3' +423 h ' ."MFBPCR"\ATV.dial :ASV F*> n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y  +43 h  F*(% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*' n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*A'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*$ % +43 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*0% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*R% h % zG ."MFBPCR"\AS.seek 0.365ml F*)' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*B % +43 h % F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.579ml F*%' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h %  ."MFBPCR"\AS.seek 0.604ml F*Q'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +21 h F*H% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*M' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*s n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Z  +22 h  ."MFBPCR"\AS.seek 0ml F*_'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F* % h %V ."MFBPCR"\AS.seek 0.01ml F*P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*[% h % ."MFBPCR"\AS.seek 0.58ml F*f' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h % q ."MFBPCR"\AS.seek 0.605ml F*\\'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +21 h F**% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*e' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g  +21 h  ."MFBPCR"\AS.seek 0ml F*:'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *9'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*t  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *m' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*C n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *T'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*h n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *='R +202 h 'R ."MFBPCR"\SPE.coast F*D  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*6 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *5' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*" I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *.'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*E  +42 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *|': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*a  +64 h  F*n% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*p n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*y I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*u  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*K  +64 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *w'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*q n +65 h n F*?% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *{' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *r': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*V n +150 h n ."MFBPCR"\AS.seek 0ml F*@'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*~% h % ."MFBPCR"\AS.seek 0.96ml F*c'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %0 ."MFBPCR"\AS.seek 0.985ml F*1' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*b% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*> I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *('R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *''R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *z'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*$% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *A' +9988 h ' ."MFBPCR"\ATV.dial AIV F*! I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*v% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*J  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *0'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*R'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*U'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\  +64 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *L'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*]' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*P'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *T'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*= % +108 h % ."MFBPCR"\AS.seek 0ml F*6'V +2547 h 'V #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:08:37CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:07:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*`% #tLoading sh1 puck from tube 2 into Collection Clamp h %V C'O& h & M&% h %\  A("% h %X A(5. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (.( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (E& h &  M& & h &- M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &a( +166 h ( M&n& h & A(|& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &p' +90 h ' A( & h &  .t\SE.seek 17.71 Pucks below Top Plate A('* +86 h '* A(y& h &  M&& h & .t\Elbow.seek 2 M&j( +6 h ( M&u& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&;'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &K/ +150 h / ."downElevator"\SE.seek :down A( 'X M&& h & M&q% h %; .t\Forearm.seek :retract M&?'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&w(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'}(W +416 h (W .tHand.open M&1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.reconfigure SSconfig .\SS.hold S)#.0X'W +2327 h 'W #t\Stroke completed -- waiting for pressure to equalize ... +50 C'% h %4 S)Y% h %W S)(% h %W .tIntake.select :close S)0 +6 )3% h %W .t\SSV.dial :exhaust S)'  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9@d +100 C'$9 h 9Dd +100 C'z9 h 9Hd +100 C'<9 h 9Ld +100 C'!9 h 9Pd +100 C'9 h 9Td +100 C'9 h 9Xd +100 C'29 h 9(\\d +100 C'v9 h 9@`d +100 C'9 h 9Wdd +100 C'9 h 9nhd +100 C'J9 h 9ld +100 C'A9 h 9pd +100 C'9 h 9td +100 C'9 h 9xd +100 C'9 h 9|d +100 C'09 h 9d +100 C'9 h 9d +100 C'/9 h 9'd +100 C' 9 h 9>d +100 C'&9 h 9Td +100 C'R9 h 9kd +100 C'B9 h 9d +100 C')9 h 9d +100 C'F9 h 9d +100 C'\ 9 h 9d +100 C'X9 h 9d +100 C'U9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C']9 h 96d +100 C'%9 h 9Ld +100 C'l9 h 9bd +100 C'N9 h 9xd +100 C'Q9 h 9d +100 C'Z9 h 9d +100 C'M9 h 9d +100 C'H9 h 9d +100 C'_9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'S9 h 9(d +100 C's9 h 9>d +100 C'9 h 9Td +100 C'L9 h 9id +100 C'[9 h 9d +100 C'^9 h 9d +100 C'i9 h 9d +100 C'P9 h 9d +100 C'W9 h 9d +100 C'*9 h 9d +100 C'\\9 h 9  d +100 C'+9 h 9 d +100 C'9 h 9 *d +100 C'g9 h 9 ?d +100 C':9 h 9 Td +100 C'f9 h 9 j d +100 C'e9 h 9 $d +100 C't9 h 9 (d +100 C'99 h 9 ,d +100 C'm9 h 9 0d +100 C'D9 h 9 4d +100 C'h9 h 9 8d +100 C'=9 h 9  ` +131 h ` #tLysing Complete C'$& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&z% h %\  A(<% h %X A(!. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&2& h &ӷ M&v% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(J& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &A' +90 h ' A(& h & .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': M&& h & A(0& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&/, +7 h , .t\Elbow.reconfigure TightElbow A( - M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(&' +26 h ' M&R% h %Y A(B& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&)'υ +21 h 'υ M&F& h & A(\ & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &X' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(U' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +89 h '\  .t\Elbow.seek CC M&%(ӷ +143 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&Q' +91 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C'M( +416 h ( A(H& h & A(_& h & M&& h &ӷ M& % h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &s( +142 h ( M&& h & A(L& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &['Y +93 h 'Y .t\Elbow.hold M&^, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&P% h %Y A(W& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&*'υ +20 h 'υ M&\\& h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' A(g% h % ."downElevator"\SE.seek :down A(:'X +100 (f% h %1 M&e% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'9 @ +2 A(6 +17 h @ .t\CS.seek 10ml C'm' +7 h ' .t\CSV.dial between air and CTV C'D ` +18 h ` .t\AIV.dial :bypass #Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'h& h & .tHand.open M&=1 +2 h 1 M&T& h & M&x% h %\  .t\Elbow.seek FlushPuck::Garage M&O(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &- .t\Forearm.seek :garage M&C'η +91 h 'η .tHand.close M&E/ +150 h / M& & h &- M&d% h %η .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'|(W +415 h (W C'& h &W .tHand.open M&`1 +150 h 1 M&u% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%4 +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.reconfigure PSconfig .\PS.seek 1.5ml P%#A d`xb %q' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%K  +38 h  .t\PS.seek 0ml P%p' +1597 h ' .t\PSV.dial PSR P%a +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'{  +76 h .t\PSV.dial :mixloop P%; +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%?' +2610 h ' .t\PSV.dial :mixloop P%j +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.seek 2.5ml P%G' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&I& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&o/ +150 h / .t\CC.seek :open C'7( +416 h ( M&8& h &ӷ M&~% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &b(- +264 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&Y1 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'.& h & M&(% h %\  A(>% h %X A($. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (z( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (<& h &  M&!& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &2( +166 h ( M&v& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(& h &  .t\SE.seek 16.71 Pucks below Top Plate A(w'; +96 h '; A(V& h &  M&& h & .t\Elbow.seek 2 M&0( +6 h ( M& & h & A(R& h &  .t\Forearm.seek 2 + 300 counts M&B'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &F/ +150 h / ."downElevator"\SE.seek :down A(\ 'X M&& h & M&% h %; .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'N(W +416 h (W .tHand.open M&)1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'/  +150 h  .t\CSV.dial CTV C'Z  +19 h  .t\CS.seek 5ml C']'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'}' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'H'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%_  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%L +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%Q  +150 h  .t\PS.seek :empty P%%' +2610 h ' .t\CBV.dial :waste1 C'i @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'P  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'W @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'+ ` +18 h ` .t\CSV.dial :air C'g @ +38 h @ .t\CS.seek 10ml C'S' +2647 h ' .t\CSV.dial CTV C's  +38 h  .t\CS.seek 0ml C'f'4 +2647 h '4 .t\CBV.dial :waste1 C'e @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'9  +151 h C'[& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&D% h %\  A(h% h %X A(=. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (T( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (x& h & M&*& h &ӷ M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A("& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &:' +90 h ' A(& h & .t\SE.seek 2.71 Pucks below Top Plate A('') +234 h ') M&^& h & A(E& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b & 'Y +26 h 'Y .t\Elbow.hold M&d, +6 h , .t\Elbow.reconfigure TightElbow A(t- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(C' +27 h ' M& % h %Y A(k& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&O'υ +20 h 'υ M&5& h & A(M& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' .t\SE.seek 4.04 Pucks below Top Plate A(`' +36 h ' M&u& h & M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&a/ +150 h / .t\CC.seek :open C'p( +416 h ( A({& h & A(;& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &j( +142 h ( M&?& h & A(r& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &G1 +150 h 1 M&I% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&o& h & A(n& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' A(|% h % ."downElevator"\SE.seek :down A(8'X +100 (~% h %  M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%@  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%Y' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%c +37 h .t\PRV.dial :air P%,  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%.  +38 h .t\CBV.dial AIV C'(  +37 h .t\PRV.dial between CBV and air P%>  +19 h  .t\PRV.dial :air P%$  +19 h  P%<% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %!' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'v& h & .tHand.open M&1 +2 h 1 M&& h & M&b% h %\  .t\Elbow.seek FlushPuck::Garage M&V(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&R'η +91 h 'η .tHand.close M&3/ +150 h / M&F& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +265 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'w(W +416 h (W C'B& h &W .tHand.open M&01 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  C'7& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%) @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%&  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%/  +75 h  .tdelay 2 seconds +200 P%Z% h % .t\PS.seek 1.499ml P%l' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%z' +1094 h ' .t\PSV.dial :air P%m  +38 h  .t\PS.seek 0ml P%]' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%} +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%_  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb % 'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%L% h %r .t\PS.seek 0.475ml P%Q' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%i'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%P  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%W  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\\' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%+ +76 h .t\PTV.dial :PRV,avoiding: :puck P%g @ +75 h @ .t\PS.seek 0ml P%H' +2610 h ' .t\PSV.dial :air P%s  +75 h  .t\PS.seek 2.5ml P%S' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%e +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%9' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%[  +75 h  .t\PS.seek 2.5ml P%D' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%h +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%=  +76 h P%x% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %*' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'U & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %"'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%X  +38 h  .t\PS.seek 2.5ml P%^' +2609 h ' .t\PSV.dial :PML P%E +38 h .t\PS.seek 0ml P%t' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 ' & h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'k& h &W F*5 @@T .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass .\PSV.dial PSR F*M  P%u +22 h  ."MFBPCR"delay 20 seconds +91 h P%4% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%:' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +21 h I F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.3ml F*#,,'Td20 b * ': +174 .t\PSV.dial :waste1 P%#  +37 h  .t\PS.seek 0ml P%K' +1597 h ' .t\PSV.dial PSR P%O +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%C' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +1480 h ': ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +43 h  F*% h %: ."MFBPCR"\AS.seek 0.33ml F*N'ї +87 h ' .t\PSV.dial PSR P%{ +113 h P%;% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +419 h 'ї ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +1156 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%r  +38 h  .t\PS.seek 0ml P%q' +2 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*G% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *I' +1430 h ' ."MFBPCR"delay 3 seconds +144 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +112 ."MFBPCR"\ATV.dial :AIV F*n I +43 h I F*|% h % ."MFBPCR"\AS.seek 0.469ml F* ' +2455 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%j +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%~' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%`  +37 h  .t\PS.seek 0ml P%@' +635 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9j #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*y n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*8% h % ."MFBPCR"\AS.seek 0.041ml F*T'% +441 h ' .t\PSV.dial PSR P%% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%c' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial PSR P%( +113 h P%>% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%<  +38 h  .t\PS.seek 0ml P%Y' +557 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +33 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%!' +123 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2487 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%b  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +106 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +954 h ' .t\PSV.dial :air P%3  +113 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %1' +5107 h ' .t\PSV.dial :air P%R  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +1222 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*p I +43 h I F*B% h %Q ."MFBPCR"\AS.seek 0.01ml F*0' +275 h ' .t\PSV.dial :mixloop P%\ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %7' +514 h ' ."MFBPCR"\ATV.dial :waste F*) n +128 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*&  +86 h  F*/% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *Z' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*l% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *m'_ +2775 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'z  +38 h  C'}% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  '  +38 h  +113  .t\CBV.dial :waste1 C'L @ +37 h @ .t\CS.seek 0ml C' '4 +1811 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*Q% h %_ ."MFBPCR"\AS.seek 0.434ml F*P'ݏ +214 h '4 .t\CSV.dial CSR C'W  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'+'P +267 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*g  +22 h F*\\% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*s'& +324 h '& ."MFBPCR"delay 3 seconds +203 h 'P .tdelay 5 seconds +97 ."MFBPCR"\ATV.dial ASV F*H n +107 h n F*e% h %& ."MFBPCR"\AS.seek 0.092ml F*S'&0 +296 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'[' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'9  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'h'4 +2647 h '4 C'=& h &W #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +6 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&*' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'"( +416 h ( M&X& h &ӷ M&% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &^(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &M'η +90 h 'η .tHand.open M&u1 +150 h 1 M&4% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'& h & M&'% h %\  A(#% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (:( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (O& h &  M&K& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &C( +166 h ( M&a& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &{' +89 h ' A(;& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A( 'L +107 h 'L A(d& h &  M&& h & ."WCR"\Elbow.seek 2 M&N( +6 h ( M&r& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&G'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +44 h '&0 ."MFBPCR"delay 3 seconds +106 h / ."downElevator"\SE.seek :down M&?& h & A('X M&n% h %; ."WCR"\Forearm.seek :retract M&|'\ +91 h '\  ."WCR"\Elbow.seek CC M&q(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +87 ."MFBPCR"\ATV.dial :waste2 F*I % +40 h (ӷ M&j& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +3 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +88 h ' ."WCR"\CC.seek :closed C'~(W +416 h (W ."WCR"Hand.open M&`1 +55 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +73 h 1 M&y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +6 h + S)%% h % C'@& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C',  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +150 h @  ."WCR"delay 3.8 seconds +380 C'.% h %4 S)<% h %5J ."WCR"\SSV.dial :spit S)$ +151 h ."WCR"\SS.seek 0ml S)' +216 h ' ."WCR"\SSV.dial CBV S)Y  +150 h  ."WCR"\CTV.dial :intake C'T  +76 h  #"WCR"Waiting for external sampler to finish priming... .delayUntil "21:36:05.06" +18487 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*!' +9452 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'b% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *$'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*c @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*T I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*b  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b * '\  +1032 h '\  F*v% h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*1'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *V' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +3472 h 'V ."MFBPCR"\SPE.coast F*R  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0122h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*B  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*!% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*p  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*Z  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*}% h %Fj ."MFBPCR"\AS.seek 0.524ml F*z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*_  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*f' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*L % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Q  +108 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*i'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*m'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*P  +43 h  ."MFBPCR"\ASV.dial :waste1 F*s  +65 h ."MFBPCR"\ARV.dial :air1 F*e  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*['{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254676 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'U  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'= @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'2 @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C'X% h % ."WCR"\CSV.dial between air and CTV C'A ` +19 h ` ."WCR"\CS.seek 7.288ml C'*' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'D'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'u  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'E  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'4  +38 h  C''% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'6' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'O' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)#% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C'M% h % ."WCR"\CSV.dial between air and CTV C'K ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C';  ."WCR"\CSV.dial CTV C'a  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '^' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C't  +37 h  C'r% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'C @ +76 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C'o% h % ."WCR"\CSV.dial between air and CTV C'I ` +56 h ` ."WCR"\CS.seek 7.288ml C'j' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'N  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'G  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'S' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'"  +151 h C'|& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&`% h %\  A(y% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (@( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&%& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &:( +143 h ( M&,& h & A(>& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &(' +89 h ' A( & h & ."WCR"\SE.seek 3.71 Pucks below Top Plate A(<' +224 h ' M&.& h & A($& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &T'Y +25 h 'Y ."WCR"\Elbow.hold M&Y, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(b' +27 h ' M&% h %Y A(& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &v'υ +21 h 'υ M& & h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &3' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +36 h ' M&& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&V& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ ."WCR"\Forearm.seek CC M&B' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'!( +416 h ( A(F& h & A(J& h & M&0& h &ӷ M&)% h % ."WCR"\Forearm.seek :retract M&&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &p( +142 h ( M&& h & A(l& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &/'Y +93 h 'Y ."WCR"\Elbow.hold M&Z, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&\ % h %Y A(_& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &]'υ +21 h 'υ M&z& h & A(L& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(f% h % ."downElevator"\SE.seek :down A(Q'X +100 (W% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&i1 +2 A(6 h 1 M&\\& h & M&g% h %\  .t\Elbow.seek FlushPuck::Garage M&m(- +166 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.seek :garage M&e'η +91 h 'η .tHand.close M&9/ +150 h / M&H& h &- M&+% h %η .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +264 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'w& h &W .tHand.open M&X1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'% h % .t\CSV.dial CSR C'*  +38 h  C'u% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'E% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '4'[ +462 h '[ .t\CSV.dial :waste C'' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'% h %4 .t\CSV.dial :flush C'#  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +37 h @ C'K% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  ';  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'd& h &W C'% h %4 .t\CSV.dial :flush C't  +75 h  .t\CS.seek 5ml C'r'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +37 h @ C'% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +38 h  .t\CBV.dial :waste2b C'n  +75 h  .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing upper manifold C'I& h &W C'?% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'N'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +37 h @ C'G% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'" '|  +38 h +75 .t\CTV.dial :air C'`  +151 h .t\CS.seek 0ml C'y'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'%'4 +507 h '4 C'& h &W C',% h %4 .t\CSV.dial :flush C'>  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +37 h @ C'8% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C''4 +506 h '4 #tDisinfecting external Intake with mfbkill C' & h &W C'% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'('L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C':' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'< +113 h .t\CTV.dial :intake C'T  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'V' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'R  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'v'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'b  +113 h  C'3% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'Y'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'~  '1  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'J'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'0  +38 h  C')% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +710 h ' ."connect"\CSV.dial CTV C'B  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'}'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'\  +37 h  C'_% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f '&  +37 h +76 .t\CTV.dial :air C'p  +150 h .t\CS.seek 0ml C'W'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'i'4 +507 h '4 #t\Flushing upper manifold C'\\& h &W C'g% h %4 .t\CSV.dial :flush C'/  +37 h  .t\CS.seek 5ml C']'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'P% h %p .t\CS.seek 7ml C's'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +507 h '0 .t\CSV.dial :waste C'Z +37 h .t\CS.seek 0ml C'9'4 +507 h '4 C'H& h &W C'+% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'h% h %0 .t\CS.seek 5ml C'm'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'x'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C'w +75 h .t\CS.seek 0ml C'X'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'A& h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'*'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'2% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  'D  +38 h  +113  .t\CBV.dial :waste1 C'' @ +37 h @ .t\CS.seek 0ml C'4'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'% h %4 .t\CSV.dial :flush C'#  +75 h  .t\CS.seek 5ml C'k'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +37 h @ C'K% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'a  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C';  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'O& h &W C'd% h %4 .t\CSV.dial CSR C'  +38 h  C't% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'^' +415 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'r  +38 h +75 .t\CTV.dial :intake C'{  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'n' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'C  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'I'4 +606 h '4 .t\CSV.dial CSR C'?  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'o'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'j' +1372 h ' .t\CSV.dial CBV C'G +113 h .t\CS.seek 0ml C'N'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +7 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&y' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&,% h % .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &%(- +264 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&.1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +90 h 'η .tHand.close M& exir !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message>undefined local variable or method `exir' for main:Object: name: exir #-> exit .#m\EXIT @1535620319.00CEST @1535620919.68CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "14sept15gordon.rb" #Hot.threshold = 30 #\Cold.threshold = 4 #\Salty.threshold = 40 #\Fresh.threshold = 25 #HighChlorophyll.threshold = 50 #LowChlorophyll.threshold = -100 #\Clear.threshold = 100 #Turbid.threshold = 0 .GoodNews.suppressMail "Starting 14sept15gordon w/pucks in tube 2\\n{}\\nCTD data unavailable\\nCan@11:22:07, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W\\nEnding 06:00CEST15-Oct-14",Subject: "14sept15gordon begun" #\Preparing to sleep until 1PM 9/15/14 #"shutdown"\Shutdown #t\Asleep til 1PM 9/15/14 #\Continuing at 13:00CEST15-Sep-14 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: fourWCRs\\n/home/esp/esp2/mission/14sept15gordon.rb:80\\n{}\\nCTD data unavailable\\nCan@11:22:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 13:00CEST15-Sep-14" @CEST1410778800.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t===> STARTED wcrOnly (WCRnumber #1) <=== #\Starting stand-alone bac WCR #bac wcr sampling at most 1000ml .BACfans.suppressMail "Started stand-alone bac WCR in tube 2 at 11:22:37CEST30-Aug-18\\n{}\\nCTD data unavailable\\nCan@11:22:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "bac wcr sampling at most 1000ml" #Whole Cell Archive sampling 1000ml #Loading wcr puck from tube 2 into Collection Clamp C'~& h & M&% h %\  A(% h %X A(. h ., A(& h &  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\ ' +89 h ' A( & h &  ."WCR"\SE.seek :upperLimit A( 'Z +271 h 'Z ."WCR"\SE.seek :down A( 'X +271 h 'X #"WCR"Tube 2 was empty. Trying tube 3 ... #Loading wcr puck from tube 3 into Collection Clamp C'& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  A(% h %X A(. h ., ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&& h & ."WCR"\Elbow.seek 3 M&( +19 h ( M&& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A(& h &~ ."WCR"\SE.seek :upperLimit A('Z +270 h 'Z ."WCR"\SE.seek :down A("'X +271 h 'X #"WCR"Tube 3 was empty. Trying tube 4 ... #Loading wcr puck from tube 4 into Collection Clamp C'& h & M&(% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +89 h '\  A(+% h %X A(,. h .,~ ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +201 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M&%& h & ."WCR"\Elbow.seek 4 M&'(k +20 h (k M&/& h &k A(0& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &1'Ю +90 h 'Ю A(2& h &,% ."WCR"\SE.seek :upperLimit A('Z +270 h 'Z ."WCR"\SE.seek :down A(4'X +271 h 'X #"WCR"Tube 4 was empty. Trying tube 5 ... #Loading wcr puck from tube 5 into Collection Clamp C'5& h & M&&% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +89 h '\  A(8% h %X A(9. h .,,% ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ()(; +201 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (6& h &; M&<& h &k ."WCR"\Elbow.seek 5 M&=(_ +20 h (_ M&3& h &_ A(:& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &.'e +90 h 'e A(@& h &; ."WCR"\SE.seek :upperLimit A(A'Z +270 h 'Z ."WCR"\SE.seek :down A(B'X +271 h 'X #"WCR"Tube 5 was empty. Trying tube 6 ... #Loading wcr puck from tube 6 into Collection Clamp C'D& h & M&E% h %e ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +89 h '\  A(G% h %X A(>. h .,; ."WCR"\SC.reconfigure SCtight .\SC.seek 6 A($\ .(\ 1~b ( (K +201 h (K ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (I& h &K M&H& h &_ ."WCR"\Elbow.seek 6 M&K(j +19 h (j M&J& h &j A(M& h &K ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 6 + 700 counts M&#Q@2^d^\ b &N'и +89 h 'и A(O& h &K ."WCR"\SE.seek :upperLimit A(L'Z +271 h 'Z ."WCR"\SE.seek :down A(R'X +270 h 'X #"WCR"Tube 6 was empty. Trying tube 7 ... #Loading wcr puck from tube 7 into Collection Clamp C'Q& h & M&S% h %и ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +90 h '\  A(U% h %X A(V. h .,K ."WCR"\SC.reconfigure SCtight .\SC.seek 7 A($\ .(\ 1~b (W([h +201 h ([h ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (X& h &[h M&T& h &j ."WCR"\Elbow.seek 7 M&Z( +21 h ( M&[& h & A(Y& h &[h ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 7 + 700 counts M&#Q@2^d^\ b &]' +89 h ' A(\\& h &[h ."WCR"\SE.seek :upperLimit A(^'Z +271 h 'Z ."WCR"\SE.seek :down A(`'X +271 h 'X ."WCR"\SE.coast A(5 M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +89 h '\  !to:Storage::Error:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Out of Pucks #-> exit #m\EXIT @1535621111.01CEST @1535621133.56CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "niagara17jul17.rb" .GoodNews.suppressMail "Starting niagara17jul17 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:25:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "niagara17jul17 begun" #\Preparing to sleep until 4PM 7/17/2017 #"shutdown"\Shutdown #t\Asleep til 4PM 7/17/2017 #\Continuing at 16:00CEST17-Jul-17 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: Sample.shallow; initialPurge; da withoutWCR\\n/home/esp/esp2/mission/niagara17jul17.rb:42\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:26:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 16:00CEST17-Jul-17" @CEST1500300000.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:26:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started niagara17jul17 phase Sample.shallow; initialPurge; da withoutWCR" #\Phase [Sample.shallow; initialPurge; da withoutWCR] !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg""uninitialized constant Sample: name: Sample #-> exit #m\EXIT @1535621207.67CEST @1535622276.06CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refip; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:44:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:21, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:45:30CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&I& ."raiseStack"\SE.seek :upperLimit h &ӷ A(E'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'\  P%  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down M&&& A(A'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %Y A(p& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%2  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%2' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  'H  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&q% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*$  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *2'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'x  ':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &$' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(2& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(7- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(1& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'x& h &W C':% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'q  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'c .t\CBV.dial CSV C'{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'&  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'$'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'A  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'N  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'x% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&$'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'H .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Preparing to sleep until #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til !o:TypeError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"$Cannot convert NilClass to Time #-> exit .#m\EXIT @1535622430.56CEST @1535622490.29CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "2018tundeploy.rb" !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"CNo '2018tundeploy.rb' script found on /home/esp/esp2/mission:. #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:56, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:49:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:49:05CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:49:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&% F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & M&I& h & ."raiseStack"\SE.seek :upperLimit h &ӷ A(E'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&&& A(A'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %Y A(p& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*O @@T .t\AWV.select :air F*t  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A( 'X M&& h & M&x% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'q  ."WCR"\CSV.dial CTV C':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'q% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV C'y  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'&  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'A  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X 'N  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C':% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV C'C  .t\CTV.dial CSV C'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C' '4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'H .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Preparing to sleep until #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til !o:TypeError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"$Cannot convert NilClass to Time #-> exit .#m\EXIT @1535622643.28CEST @1535623283.86CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "14aprEcohab.rb" #Hot.threshold = 25 #\Cold.threshold = 4.15 #\Salty.threshold = 35 #\Fresh.threshold = 30 #LowChlorophyll.threshold = 2 #HighChlorophyll.threshold = 8 .GoodNews.suppressMail "Starting 14aprEcohab w/pucks in tube 2\\n{}\\nCTD data unavailable\\nCan@12:01:31, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W\\nEnding 08:00CEST17-May-14",Subject: "14aprEcohab begun" #\Preparing to sleep until 4/1/14 12:00PM #"shutdown"\Shutdown #t\Asleep til 4/1/14 12:00PM #\Continuing at 12:00CEST01-Apr-14 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: ecohabda\\n/home/esp/esp2/mission/14aprEcohab.rb:46\\n{}\\nCTD data unavailable\\nCan@12:01:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 12:00CEST01-Apr-14" #"triggerCondition"awaiting Equilibrated Trigger holdoff:false, threshold:3.5, type:Temperature, test:(CTD.temperature - Gate.can.temperature).abs <= threshold !o:NameError :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"9uninitialized constant Trigger::Mixin::SensorPolling: name:SensorPolling:@timestampu: Time  .BadNews.suppressMail "uninitialized constant Trigger::Mixin::SensorPolling",Subject: "NameError in triggerCondition" #Waiting to Resume from Checkpoint "awaiting Equilibrated Trigger holdoff:false, threshold:3.5, type:Temperature, test:(CTD.temperature - Gate.can.temperature).abs <= threshold" @CEST1396346400.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X STARTED ecohabda #1 <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'Q .t\CBV.dial CSV C'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting hab #\hab sampling at most 1000ml .HABfans.suppressMail "Started hab in tube 2 at 12:01:53CEST30-Aug-18\\n{}\\nCTD data unavailable\\nCan@12:01:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "hab sampling at most 1000ml" #Loading sh1 puck from tube 2 into Collection Clamp C'~& h & M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\ ' +89 h ' A( & h &  .t\SE.seek :upperLimit A( 'Z +271 h 'Z .t\SE.seek :down A( 'X +271 h 'X #tTube 2 was empty. Trying tube 3 ... #Loading sh1 puck from tube 3 into Collection Clamp C'& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&& h & .t\Elbow.seek 3 M&( +19 h ( M&& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A(& h &~ .t\SE.seek :upperLimit A('Z +270 h 'Z .t\SE.seek :down A("'X +271 h 'X #tTube 3 was empty. Trying tube 4 ... #Loading sh1 puck from tube 4 into Collection Clamp C'& h & M&(% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +89 h '\  A(+% h %X A(,. h .,~ .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +201 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M&%& h & .t\Elbow.seek 4 M&'(k +20 h (k M&/& h &k A(0& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &1'Ю +90 h 'Ю A(2& h &,% .t\SE.seek :upperLimit A('Z +270 h 'Z .t\SE.seek :down A(4'X +271 h 'X #tTube 4 was empty. Trying tube 5 ... #Loading sh1 puck from tube 5 into Collection Clamp C'5& h & M&&% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +89 h '\  A(8% h %X A(9. h .,,% .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ()(; +201 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (6& h &; M&<& h &k .t\Elbow.seek 5 M&=(_ +20 h (_ M&3& h &_ A(:& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &.'e +90 h 'e A(@& h &; .t\SE.seek :upperLimit A(A'Z +270 h 'Z .t\SE.seek :down A(B'X +271 h 'X #tTube 5 was empty. Trying tube 6 ... #Loading sh1 puck from tube 6 into Collection Clamp C'D& h & M&E% h %e .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +89 h '\  A(G% h %X A(>. h .,; .t\SC.reconfigure SCtight .\SC.seek 6 A($\ .(\ 1~b ( (K +201 h (K .t\SC.reconfigure SCloose A($.(\ 1~b (I& h &K M&H& h &_ .t\Elbow.seek 6 M&K(j +19 h (j M&J& h &j A(M& h &K .t\Forearm.reconfigure TightForearm .\Forearm.seek 6 + 700 counts M&#Q@2^d^\ b &N'и +89 h 'и A(O& h &K .t\SE.seek :upperLimit A(L'Z +271 h 'Z .t\SE.seek :down A(R'X +270 h 'X #tTube 6 was empty. Trying tube 7 ... #Loading sh1 puck from tube 7 into Collection Clamp C'Q& h & M&S% h %и .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +90 h '\  A(U% h %X A(V. h .,K .t\SC.reconfigure SCtight .\SC.seek 7 A($\ .(\ 1~b (W([h +201 h ([h .t\SC.reconfigure SCloose A($.(\ 1~b (X& h &[h M&T& h &j .t\Elbow.seek 7 M&Z( +21 h ( M&[& h & A(Y& h &[h .t\Forearm.reconfigure TightForearm .\Forearm.seek 7 + 700 counts M&#Q@2^d^\ b &]' +89 h ' A(\\& h &[h .t\SE.seek :upperLimit A(^'Z +271 h 'Z .t\SE.seek :down A(`'X +271 h 'X .t\SE.coast A(5 M&_% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +89 h '\  !to:Storage::Error:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Out of Pucks #-> exit #m\EXIT @1535623344.00CEST @1535624908.19CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "14aprEcohab.rb" #Hot.threshold = 25 #\Cold.threshold = 4.15 #\Salty.threshold = 35 #\Fresh.threshold = 30 #LowChlorophyll.threshold = 2 #HighChlorophyll.threshold = 8 .GoodNews.suppressMail "Starting 14aprEcohab w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:28:36, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W\\nEnding 08:00CEST17-May-14",Subject: "14aprEcohab begun" #\Preparing to sleep until 4/1/14 12:00PM #"shutdown"\Shutdown #t\Asleep til 4/1/14 12:00PM #\Continuing at 12:00CEST01-Apr-14 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: ecohabda\\n/home/esp/esp2/mission/14aprEcohab.rb:48\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:29:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 12:00CEST01-Apr-14" #"triggerCondition"awaiting Equilibrated Trigger holdoff:false, threshold:3.5, type:Temperature, test:(CTD.temperature - Gate.can.temperature).abs <= threshold !o:NameError :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"9uninitialized constant Trigger::Mixin::SensorPolling: name:SensorPolling:@timestampu: Time  .BadNews.suppressMail "uninitialized constant Trigger::Mixin::SensorPolling",Subject: "NameError in triggerCondition" #Waiting to Resume from Checkpoint "awaiting Equilibrated Trigger holdoff:false, threshold:3.5, type:Temperature, test:(CTD.temperature - Gate.can.temperature).abs <= threshold" @CEST1396346400.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:29:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 14aprEcohab phase ecohabda" #\Phase [ecohabda] #===> STARTED ecohabda #1 <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting hab #\hab sampling at most 1000ml .HABfans.suppressMail "Started hab in tube 2 at 12:29:13CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:29:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "hab sampling at most 1000ml" #Loading sh1 puck from tube 2 into Collection Clamp C'~& h & M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\ ' +89 h ' A( & h &  .t\SE.seek 20.71 Pucks below Top Plate A( ' +57 h ' A( & h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'"(W +416 h (W .tHand.open M&1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  .t\SC.coast #collectSample 1000ml w/bubble point of 24psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)++ +6 h + S),% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +800 h ' .t\Exhaust.select :close S) +147794 #t\Sampled 1000.0ml #\Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C' & h &W ."airStroke"\CSV.dial :waste S)I% h % ."connect"\SSV.dial CBV,avoiding: :exhaust ."airStroke"\CS.seek 0ml S)H  .t\CBV.dial SSV C'K'4 +7 h '4 ."airStroke"\CSV.dial :air C'J @ +75 h @ ."airStroke"\CS.seek 10ml C'M' +144 h  .t\CTV.dial :air C'N  +75 h .tdelay 15 seconds +691 h ' +809 .t\SSV.dial :spit,avoiding: :exhaust S)O +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.seek 0ml C'L  S)R' +6 h ' .t\SSV.dial CBV,avoiding: :exhaust S)Q  +144 h  +7  S)S% h % .t\CBV.dial between SSV and waste2 C'P  +19 h  C'U% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'V  +37 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<1b 'W'4 +2610 h '4 C'X% h %4 S)T% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'Z @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X% h % ."blocking"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +44 C'X9 h 9kd +100 C'9 h 9Td +100 C'9 h 9kd +100 C' 9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'|9 h 9d +100 C'w9 h 9d +83 'Rq ."blocking"delay 5 seconds +17 C'z9 h 9 d +100 C'9 h 9 d +100 C'9 h 96d +100 C'}9 h 9Ld +100 C'9 h 9bd +83 ."blocking"\PSR.select :air P% +6 ."blocking"\PS.seek 2.5ml P%' +11 C'9 h 9xd +100 C'9 h 9d +100 C'#9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'\ 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C' 9 h 9Td +100 C's9 h 9id +100 C'9 h 9d +100 C'(9 h 9d +100 C'9 h 9d +100 C'9 h 9d +86 ' ."blocking"\PTV.dial :puck .\PSV.dial PTV P% +14 C'!9 h 9d +61 ."blocking"\PBV.dial :waste1 P%+  +39 C'%9 h 9d +62  P%/% h % ."blocking"\PS.seek 2ml P%0' +38 C'19 h 9  d +100 C'9 h 9 d +100 C'49 h 9 *d +100 C'59 h 9 ?d +100 C'79 h 9 Td +100 C'89 h 9 j d +47 ' ."blocking"delay 3 seconds +53 C')9 h 9 $d +100 C'69 h 9 (d +100 C'&9 h 9 ,d +47 ."blocking"\PBV.dial between PSV and waste1 P% * +24 h * ."blocking"\PS.seek 0.25ml P%:' +29 C'.9 h 9 0d +100 C'=9 h 9 4d +100 C'*9 h 9 8d +100 C'<9 h 9 9 h 9 d +100 C'X9 h 9 d +100 C'T9 h 9 d +100 C'R9 h 9 d +100 C'Z9 h 9 1d +100 C']9 h 9 Dd +100 C'\\9 h 9 Xd +100 C'[9 h 9 kd +100 C'N9 h 9 ~d +100 C'^9 h 9 d +100 C'_9 h 9 d +100 C''9 h 9 d .t\CH.hold 5:00,85C C'b   P h  +28022 #"blocking"recoverReagent out waste1 .\PS.seek 0ml P%d' +331 h ' ."blocking"\PSV.dial PBV P%` @ +38 h @ ."blocking"\PBV.dial PSV P%f U +25 h U ."blocking"\PS.seek 2.5ml P%W' +1584 h  C'a9 h 9  #t\Collection Puck Heater at 85C after lysing +1026 h ' ."blocking"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%c +38 h ."blocking"delay 2 seconds +200 .\PSV.dial :waste1 P%g  +37 h  ."blocking"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %h' +1047 h ' #"blocking"Release puck top pressure .\PSV.dial :air P%i  +113 h  ."blocking"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %j' +2610 h ' ."blocking"delay 2 seconds +200 .\PSV.dial PTV P%m +75 h ."blocking"delay 2 seconds +200 .\PSV.dial :air P%e  +76 h  ."blocking"delay 2 seconds +200 .\PSV.dial PTV P%o +75 h ."blocking"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%p  +51 h  ."blocking"delay 2 seconds +200 .\PSV.dial PBV P%; @ +37 h @ ."blocking"delay 2 seconds +200 .\PSV.dial PSR P%k +38 h ."blocking"delay 2 seconds +200 .\PSV.dial PBV P%r @ +37 h @ ."blocking"delay 2 seconds +200 .\PSV.dial PSR P%? +38 h ."blocking"delay 2 seconds +200 .\PSV.dial :waste1 P%t  +38 h  ."blocking"\PS.seek 0ml P%q' +2609 h ' .t\CS.seek 0ml C'x'4 +351 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'y @ +113 h @ .t\CSV.dial :air C'n @ +37 h @ .t\CS.seek 5ml C'{'p +1373 h 'p .t\CSV.dial CTV C'C  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'~  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'u @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'  +19 h C'% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C' % h %p .t\CS.seek 7ml C''i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +37 h @ .t\CS.seek 10ml C'|' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'w ` +132 h ` #tLysing Complete C'z& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&% h %\  A(% h %X A(}. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& ."raiseStack"\SE.seek :upperLimit h &w A(#'Z M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +271 h 'Z +65 (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A( & h & M&s& h &ӷ M&% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(!& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&%, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &+1 +150 h 1 M&/% h %Y A(1& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&4& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &5' +22 h ' A(7% h %Z ."downElevator"\SE.seek :down A(8'X +100 ()% h % M&6% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tBeginning samplePassA #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&.1 + A(6 + h 1 M&=& h & M&*% h %\  .t\Elbow.seek FlushPuck::Garage M&0(- +165 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.seek :garage M&E'η +91 h 'η .tHand.close M&G/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +265 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &J' +91 h ' .t\CC.seek :closed C'H(W +416 h (W C'l& h &W .tHand.open M&D1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  .t\PSV.dial PSR P%L +37 h P%Q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%F' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%S  +37 h  .t\PS.seek 0ml P%-' +1598 h ' .t\PSV.dial PSR P%P +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%V' +2609 h ' ."connect"\PSV.dial CBV P%U @ .t\CBV.dial PSV C'>  +113 h @ .t\CTV.dial :air .\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial :air P%T  +38 h  .t\PS.seek 2.5ml P%R' +2609 h ' .t\PSV.dial CBV P%Z @ +38 h @ .t\PS.seek 0ml P%]' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&\\& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +6 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&'/ +150 h / .t\CC.seek :open C'Y( +416 h ( M&:& h &ӷ M&`% h % .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &d(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &c'η +91 h 'η .tHand.open M&g1 +150 h 1 M&W% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #tLoading lyfil puck from tube 2 into Collection Clamp C'h& h & M&m% h %\  A(e% h %X A(o. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (p( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (;& h &  M&k& h &- M&r% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &?( +166 h ( M&$& h & A(t& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &j' +90 h ' A(,& h &  .t\SE.seek 18.71 Pucks below Top Plate A(y' +77 h ' A(n& h &  M&x& h & .t\Elbow.seek 2 M&C( +6 h ( M&& h & A(~& h &  .t\Forearm.seek 2 + 300 counts M&u'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &{/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M& (ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&w1 +150 h 1 M&z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #Beginning samplePassB A(6 E ;3\\0\\0 #t\Calibrating pressure sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X(ӷ +142 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&X/ +150 h / .t\CC.seek :open C'Z( +416 h ( A(R& h & A(\\& h & M&N& h &ӷ M&^% h % .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &I( +142 h ( M&K& h & A(H& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &:'Y +93 h 'Y .t\Elbow.hold M&`, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &_1 +150 h 1 M&b% h %Y A(a& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&['υ +21 h 'υ M&g& h & A(W& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &f' +22 h ' A(h% h % ."downElevator"\SE.seek :down A(m'X +100 (e% h %S M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast #\Starting SH2 of hab using sig1 .\PTV.dial :puck .\PBV.dial :air .\PTV.dial :puck .\PSV.dial PTV P%k +2 A(6 +73 h .t\PBV.dial :waste1 P%r  +101 h  P%c% h % .t\PS.seek 2ml P%$' +585 h ' .tdelay 3 seconds +300 .\PBV.dial between PSV and waste1 P%t * +24 h * .t\PS.seek 0.25ml P%,' +1851 h ' #tBeginning samplePassA #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'd& h & .tHand.open M&n1 +2 h 1 M&x& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&~(- +166 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&?& h &- M&j% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'& h &W .tHand.open M&Y1 +150 h 1 M&w% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  .t\PSV.dial PSR P% +75 h P%% h % #t\Pulling 1.25ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%p' +1344 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%}  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%A' +2610 h ' ."connect"\PSV.dial CBV .t\CBV.dial PSV P% @ C'\  +113 h @ .t\CTV.dial :air .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%"  +37 h  .t\PS.seek 2.5ml P%M' +2610 h ' .t\PSV.dial CBV P% @ +38 h @ .t\PS.seek 0ml P%]' +2609 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C's' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '|  +37 h  +113  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'+'4 +2647 h '4 .t\CSV.dial CSR C'  +38 h  C'1% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'/'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C'6( +416 h ( M&*& h &ӷ M&% h % .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +264 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&G1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  ."sh2"delay 20:28 #t\Skipping Whole Cell Archive +122800 #"sh2"recoverReagent out waste1 .\PS.seek 0ml P% ' +6 h ' ."sh2"\PSV.dial PBV P% @ +151 h @ ."sh2"\PBV.dial PSV P%9 U +25 h U ."sh2"\PS.seek 2.5ml P%@' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P% +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%D  +37 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %3' +1048 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%Q  +112 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%F +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%P  +76 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%%  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%E @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%0 +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%U @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%V +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%L  +38 h  ."sh2"\PS.seek 0ml P%l' +2610 h ' ."sh2"\PSR.select :air .\PSV.dial PSR P%q +37 h P%2% h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +7 ."sh2"\PS.seek 1ml P%X'Rq +1090 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +7 ."sh2"\PS.seek 2.5ml P%-' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P% +75 h ."sh2"\PBV.dial :waste1 P%R  +101 h  P%\\% h % ."sh2"\PS.seek 2ml P%N' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%^ * +24 h * ."sh2"\PS.seek 0.25ml P%K' +1851 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%H' +331 h ' ."sh2"\PSV.dial PBV P%T @ +38 h @ ."sh2"\PBV.dial PSV P%> U +25 h U ."sh2"\PS.seek 2.5ml P%_' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%b +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%a  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %g' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%W  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %h' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%' +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%I  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%e +76 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%o  +50 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%: @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%[ +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%S @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%k +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%f  +38 h  ."sh2"\PS.seek 0ml P%r' +2609 h ' #"sh2"\Adding signal .\PSR.select :air .\PSV.dial PSR P%c +38 h P%<% h % #"sh2"\Pulling 1ml of PSR.sig1 .\PSR.select :sig1 P% +6 ."sh2"\PS.seek 1ml P%m'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +7 ."sh2"\PS.seek 2.5ml P%4' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%t +75 h ."sh2"\PBV.dial :waste1 P%$  +101 h  P%d% h % ."sh2"\PS.seek 2ml P%n' +584 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%x * +25 h * ."sh2"\PS.seek 0.25ml P%' +1850 h ' ."sh2"delay 10:00 +60000 #recoverReagent out waste1 .\PS.seek 0ml P%i' +332 h ' ."sh2"\PSV.dial PBV P%{ @ +38 h @ ."sh2"\PBV.dial PSV P%` U +25 h U ."sh2"\PS.seek 2.5ml P%' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%? +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%j  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %;' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %u' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%y +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%~  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +76 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%Z  +50 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P% @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%Y +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%w @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P% +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%  +37 h  ."sh2"\PS.seek 0ml P%' +2610 h ' #"sh2"sh2wash #1 of 2 .\PSR.select :air .\PSV.dial PSR P%, +38 h P%}% h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +6 ."sh2"\PS.seek 1ml P%p'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%' +1598 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P% +75 h ."sh2"\PBV.dial :waste1 P%\  +100 h  P%% h % ."sh2"\PS.seek 2ml P%A' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%" * +25 h * ."sh2"\PS.seek 0.25ml P%' +1850 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%' +332 h ' ."sh2"\PSV.dial PBV P%M @ +37 h @ ."sh2"\PBV.dial PSV P% U +26 h U ."sh2"\PS.seek 2.5ml P%' +2609 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%= +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%|  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +2609 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%s +76 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%1 +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%+  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%/ @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%7 +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P% @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%) +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%(  +38 h  ."sh2"\PS.seek 0ml P%' +2610 h ' #"sh2"sh2wash #2 of 2 .\PSR.select :air .\PSV.dial PSR P%. +37 h P%% h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +7 ."sh2"\PS.seek 1ml P%z'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%C' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%* +76 h ."sh2"\PBV.dial :waste1 P%  +100 h  P%% h % ."sh2"\PS.seek 2ml P%B' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P% * +25 h * ."sh2"\PS.seek 0.25ml P%!' +1850 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%G' +331 h ' ."sh2"\PSV.dial PBV P% @ +38 h @ ."sh2"\PBV.dial PSV P%# U +25 h U ."sh2"\PS.seek 2.5ml P%8' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P% +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%9  +37 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %]' +1048 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%  +112 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%D +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%@  +76 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%Q +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%3  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%F @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%P +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P% @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%% +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%E  +38 h  ."sh2"\PS.seek 0ml P%0' +2610 h ' #"sh2"\Adding conjugate .\PSR.select :air .\PSV.dial PSR P%U +37 h P%V% h % #"sh2"\Pulling 1ml of PSR.conjugate .\PSR.select :conjugate P% +7 ."sh2"\PS.seek 1ml P%L'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%J' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%q +75 h ."sh2"\PBV.dial :waste1 P%2  +101 h  P%l% h % ."sh2"\PS.seek 2ml P%X' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P% * +24 h * ."sh2"\PS.seek 0.25ml P%R' +1851 h ' ."sh2"delay 5:00 +30000 #recoverReagent out waste1 .\PS.seek 0ml P%\\' +331 h ' ."sh2"\PSV.dial PBV P%- @ +38 h @ ."sh2"\PBV.dial PSV P%^ U +25 h U ."sh2"\PS.seek 2.5ml P%N' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%K +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%T  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %>' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%H  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %b' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%a +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%_  +76 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%W +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%g  +50 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%' @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%I +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%e @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%o +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%:  +38 h  ."sh2"\PS.seek 0ml P%[' +2609 h ' #"sh2"sh2wash #1 of 4 .\PSR.select :air .\PSV.dial PSR P%S +38 h P%k% h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +6 ."sh2"\PS.seek 1ml P%f'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +7 ."sh2"\PS.seek 2.5ml P%h' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%c +75 h ."sh2"\PBV.dial :waste1 P%<  +101 h  P%r% h % ."sh2"\PS.seek 2ml P%m' +584 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%t * +25 h * ."sh2"\PS.seek 0.25ml P%$' +1850 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%d' +332 h ' ."sh2"\PSV.dial PBV P%4 @ +38 h @ ."sh2"\PBV.dial PSV P%x U +25 h U ."sh2"\PS.seek 2.5ml P%n' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P% +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%{  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %`' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%i  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %?' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%j +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +76 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%;  +50 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%y @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%~ +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P% @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%Z +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%  +37 h  ."sh2"\PS.seek 0ml P%Y' +2610 h ' #"sh2"sh2wash #2 of 4 .\PSR.select :air .\PSV.dial PSR P%w +38 h P% % h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +6 ."sh2"\PS.seek 1ml P%'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%u' +1598 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%, +75 h ."sh2"\PBV.dial :waste1 P%}  +100 h  P%% h % ."sh2"\PS.seek 2ml P%p' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P% * +25 h * ."sh2"\PS.seek 0.25ml P%\ ' +1850 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%' +332 h ' ."sh2"\PSV.dial PBV P% @ +37 h @ ."sh2"\PBV.dial PSV P%" U +26 h U ."sh2"\PS.seek 2.5ml P%A' +2609 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P% +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%M  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb % ' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %=' +2609 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +76 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%| +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%s @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P% +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%1 @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%+ +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%/  +38 h  ."sh2"\PS.seek 0ml P%7' +2610 h ' #"sh2"sh2wash #3 of 4 .\PSR.select :air .\PSV.dial PSR P% +37 h P%)% h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +7 ."sh2"\PS.seek 1ml P%('Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%. +76 h ."sh2"\PBV.dial :waste1 P%  +100 h  P%% h % ."sh2"\PS.seek 2ml P%z' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%* * +25 h * ."sh2"\PS.seek 0.25ml P%' +1850 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%' +331 h ' ."sh2"\PSV.dial PBV P%C @ +38 h @ ."sh2"\PBV.dial PSV P% U +25 h U ."sh2"\PS.seek 2.5ml P%B' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%! +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%  +37 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %#' +1048 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%G  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +2609 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%9 +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%8  +76 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%]  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%D @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%@ +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%Q @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%3 +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%F  +38 h  ."sh2"\PS.seek 0ml P%P' +2610 h ' #"sh2"sh2wash #4 of 4 .\PSR.select :air .\PSV.dial PSR P% +37 h P%%% h % #"sh2"\Pulling 1ml of PSR.wash .\PSR.select :wash P% +7 ."sh2"\PS.seek 1ml P%E'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%U +75 h ."sh2"\PBV.dial :waste1 P%V  +101 h  P%0% h % ."sh2"\PS.seek 2ml P%L' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%q * +24 h * ."sh2"\PS.seek 0.25ml P%2' +1851 h ' ."sh2"delay 2:00 +12000 #recoverReagent out waste1 .\PS.seek 0ml P%l' +331 h ' ."sh2"\PSV.dial PBV P%J @ +38 h @ ."sh2"\PBV.dial PSV P% U +25 h U ."sh2"\PS.seek 2.5ml P%X' +2610 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%R +37 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%-  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %^' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%\\  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %K' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%T +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%N  +76 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%H +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%>  +50 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%a @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%_ +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%W @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%g +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%'  +38 h  ."sh2"\PS.seek 0ml P%I' +2609 h ' ."sh2"Gate.power :camera,:ON #\Mixing sh2 Substrates .\PSV.dial PSR P%e +38 h P%o% h % #"sh2"\Pulling 0.5ml of PSR.sub1 .\PSR.select :sub1 P%@ +6 ."sh2"\PS.seek 0.5ml P%:'8 +585 h '8 ."sh2"delay 2 seconds +200 P%b% h %8 #"sh2"\Pulling 0.5ml of PSR.sub2 .\PSR.select :sub2 P% +6 ."sh2"\PS.seek 1ml P%S'Rq +585 h 'Rq ."sh2"delay 2 seconds +200 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%k' +1598 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P%[ +75 h ."sh2"\PBV.dial :waste1 P%f  +100 h  P%c% h % ."sh2"\PS.seek 2ml P%<' +585 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%r * +25 h * ."sh2"\PS.seek 0.25ml P%h' +1850 h ' ."sh2"delay 7 seconds +700 #recoverReagent out waste1 .\PS.seek 0ml P%t' +332 h ' ."sh2"\PSV.dial PBV P%m @ +37 h @ ."sh2"\PBV.dial PSV P%$ U +26 h U ."sh2"\PS.seek 2.5ml P%4' +2609 h ' ."sh2"delay 4 seconds +400 .\PSR.select :air .\PSV.dial PSR P%x +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%d  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%{  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %n' +2609 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%i +76 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%`  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%j +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P% @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%; +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%y @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%~ +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%  +37 h  ."sh2"\PS.seek 0ml P%Z' +2610 h ' P%9 h 9a #"sh2"\Processing Puck Heater at 29C before imaging .\PH.coast P%?  h a P%w& h &T #"sh2"Imaging sh2 puck in Processing Clamp while storing it to tube 1 M& % h %\  A(Y% h %X A(. h . ."sh2"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (,( +6 h ( ."sh2"\SC.reconfigure SCloose A($.(\ 1~b (}& h & M&& h &- M&u% h %\  ."sh2"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +166 h ( M&p& h & A(\ & h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +90 h ' A("& h & ."sh2"\SE.seek 0.71 Pucks below Top Plate A('K +253 h 'K M&& h & A(M& h & ."sh2"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."sh2"\Forearm.seek 1 M&#Q@2^d^2b &A'Y +26 h 'Y ."sh2"\Elbow.hold M&, +6 h , ."sh2"\Elbow.reconfigure TightElbow A( - M&$&.,(\ #b h -+K ."sh2"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&% h %Y A(|& h & ."sh2"\Forearm.force -20 M&5 ."sh2"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&s& h & A(& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +22 h ' ."sh2"\SE.seek 2.04 Pucks below Top Plate A(+' +37 h ' M&/& h & M&=% h % ."sh2"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."sh2"\Elbow.seek PC M&)(w +236 h (w M&7& h &w ."sh2"\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &w ."sh2"\Forearm.seek PC M&.' +91 h ' ."sh2"Hand.close M&/ +150 h / ."sh2"\PC.seek :open P%( +404 h ( A(& h & M&*& h &w M&z% h % ."sh2"\Forearm.seek :retract M&'\ +91 h '\  ."sh2"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +236 h ( M&& h & A(& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &!' +89 h ' ."sh2"Hand.open M& 1 +150 h 1 A(B- h -+ ."sh2"\SE.seek 1.79 Pucks below Top Plate A(G' +25 h ' ."sh2"\SE.reconfigure SEseal .\SE.seek 1.09 Pucks below Top Plate A(#QK-0-\ b (#'/ +48 h '/ #"sh2"Lights, camera, ACTION! #\Simulating PuckCamera: snap -bin=1,1 160.0 /var/log/esp/hires/hab14apr0114h25ml160s.tif .delay 2:50 +2000 #"psFlush"Waiting >20 seconds for sh2 to relinquish FlushPuck +15000 #"sh2"\Simulating PuckCamera: snap -bin=2,2 40.0 /var/log/esp/hab14apr0114h25ml40s.tif .delay 50 seconds +5000 #\Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/midres/hab14apr0114h25ml.tif .delay 1:50 +11000 .HABfans.suppressMail "110.0 sec auto-exposure",Subject: "hab14apr0114h25ml images!" .Gate.power :camera,:OFF A(9- h -+/ A(8& h & M& & h & ."sh2"\Elbow.seek 1 M&]( +6 h ( M&D& h & A(@& h & ."sh2"\Forearm.seek 1 + 700 counts M&Q' +7 h ' ."sh2"\SE.reconfigure SEgently .\SE.seek 1.81 Pucks below Top Plate A(#QK-0-\ b (3' +47 h ' M&F& h & A(& h & ."sh2"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."sh2"\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y ."sh2"\Elbow.hold M&%, +7 h , ."sh2"\Elbow.reconfigure TightElbow .\SE.reconfigure SEconfig M&$&.,(\ #b ."sh2"\SE.seek 1.99 Pucks below Top Plate A(#QK-0-\ b (P' +24 h ' M&E% h %Y A(U& h & ."sh2"\Forearm.force -20 M&5 ."sh2"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &V'υ +21 h 'υ M&0& h & A(& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &q' +22 h ' A(L% h % ."downElevator"\SE.seek :down A(2'X +100 (J% h % M&% h % ."sh2"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."sh2"\SC.coast #"psFlush"Loading FlushPuck into Processing Clamp from FlushPuck::Garage #t\Starting finalPurge P%R& h & ."psFlush"Hand.open M&-1 + A(6 + h 1 M&X& h & M&\\% h %\  ."psFlush"\Elbow.seek FlushPuck::Garage M&^(- +166 h (- M&T& h &- ."psFlush"\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- ."psFlush"\Forearm.seek :garage M&H'η +91 h 'η ."psFlush"Hand.close M&>/ +150 h / M&a& h &- M&_% h %η ."psFlush"\Forearm.seek :retract M&W'\ +91 h '\  ."psFlush"\Elbow.reconfigure TightElbow .\Elbow.seek PC M&$&.,(\ #b &g(w +114 h (w M&'& h &w ."psFlush"\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &w ."psFlush"\Forearm.reconfigure TightForearm .\Forearm.seek PC M&#Q@2^d^\ b &e' +91 h ' ."psFlush"\PC.seek :closed P%o(T +403 h (T P%I& h &T ."psFlush"Hand.open M&b1 +150 h 1 M&:% h % ."psFlush"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  ."psFlush"\PSV.dial PSR P%[ +38 h P%f% h % #"psFlush"\Pulling 0.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 ."psFlush"\PS.seek 0.5ml P%c'8 +585 h '8 ."psFlush"delay 5 seconds +22 #tWaiting >20 seconds for psFlush to relinquish FlushPuck +478 ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%k' +2104 h ' ."psFlush"\PTV.dial :puck .\PSV.dial PTV P%r +75 h ."psFlush"\PBV.dial :waste1 P%<  +101 h  ."psFlush"\PS.seek :empty P%h' +2609 h ' ."psFlush"\PSV.dial PSR P%m +76 h ."psFlush"\PSR.select :flush P%@ +6 ."psFlush"\PS.seek 1ml P%$'Rq +1094 h 'Rq ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%t' +1598 h ' ."psFlush"\PSV.dial PTV P%x +75 h ."psFlush"\PTV.dial :puck .\PBV.dial :waste1 .\PS.seek 0ml P%d' +2610 h ' ."psFlush"\PSV.dial PSR P%4 +75 h ."psFlush"\PSR.select :flush P%@ +6 ."psFlush"\PS.seek 0.5ml P%{'8 +585 h '8 ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%' +2104 h ' ."psFlush"\PSV.dial PTV P%i +75 h ."psFlush"\PBV.dial PSV P%` U +50 h U ."psFlush"\PS.seek 0ml P%j' +2610 h ' ."psFlush"\PSV.dial PBV P% @ +37 h @ ."psFlush"\PS.seek 2.5ml P%' +2610 h ' ."psFlush"\PSV.dial :waste1 P%;  +75 h  ."psFlush"\PS.seek :empty P%y' +2610 h ' ."psFlush"\PSV.dial PSR P%~ +38 h ."psFlush"\PSR.select :air .\PS.seek 2.5ml P% ' +2609 h ' ."psFlush"\PSV.dial PTV P%n +76 h ."psFlush"\PBV.dial PSV .\PS.seek 0ml P%' +2609 h ' ."psFlush"\PSV.dial PBV P%? @ +38 h @ ."psFlush"\PS.seek 2.5ml P%w' +2610 h ' ."psFlush"\PSV.dial :waste1 P%  +75 h  ."psFlush"\PS.seek :empty P%Y' +2610 h ' ."psFlush"\PSV.dial PSR P% +37 h ."psFlush"\PSR.select :flush P%@ +7 ."psFlush"\PS.seek 1ml P%}'Rq +1094 h 'Rq ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%' +1597 h ' ."psFlush"\PSV.dial PTV P%u +76 h ."psFlush"\PTV.dial :puck .\PBV.dial :waste1 P%p  +50 h  ."psFlush"\PS.seek 0ml P%\ ' +2609 h ' ."psFlush"\PSV.dial PSR P%" +76 h ."psFlush"\PSR.select :flush P%@ +6 ."psFlush"\PS.seek 0.5ml P%5'8 +585 h '8 ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%&' +2103 h ' ."psFlush"\PSV.dial PTV P% +76 h ."psFlush"\PBV.dial PSV P%M U +50 h U ."psFlush"\PS.seek 0ml P% ' +2609 h ' ."psFlush"\PSV.dial PBV P% @ +38 h @ ."psFlush"\PS.seek 2.5ml P%|' +2610 h ' ."psFlush"\PSV.dial :waste1 P%s  +75 h  ."psFlush"\PS.seek :empty P%' +2610 h ' ."psFlush"\PSV.dial PSR P%/ +37 h ."psFlush"\PSR.select :air .\PS.seek 2.5ml P%=' +2610 h ' ."psFlush"\PSV.dial PTV P% +75 h ."psFlush"\PBV.dial PSV .\PS.seek 0ml P%' +2610 h ' ."psFlush"\PSV.dial PBV P% @ +38 h @ ."psFlush"\PS.seek 2.5ml P%A' +2609 h ' ."psFlush"\PSV.dial :waste1 P%  +76 h  ."psFlush"\PS.seek :empty P%' +2609 h ' #tLoading FlushPuck into Collection Clamp from Processing Clamp C'1& h & .tHand.open M&71 +2 h 1 M&(& h &w .t\Elbow.reconfigure TightElbow .\Elbow.seek PC M&$&.,(\ #b &(w +7 h (w M&& h &w .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &w .t\Forearm.seek PC M&O' +91 h ' .tHand.close M&/ +150 h / .t\PC.seek :open P%*( +403 h ( M&z& h &w M&.% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +336 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'+& h &W .tHand.open M&G1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'D% h %4 .t\CSV.dial CSR C'@  +38 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'E% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'U'[ +463 h '[ .t\CSV.dial :waste C'0 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'L& h &W C'J% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! '#  +38 h +38 .t\CTV.dial :intake C'Q  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C']'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'3  +75 h #t\Flushing upper manifold C'R& h &W C'-% h %4 .t\CSV.dial :flush C'X  +76 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'V% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'q +113 h .t\CTV.dial :air .\CS.seek 0ml C'T'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'N'0 +506 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C','4 +506 h '4 C'%& h &W C'>% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C'_'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C''% h %0 .t\CS.seek 5ml C'K'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'H'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'W +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste2b port C'I& h &W C'b% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'e% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  'r  +38 h  +113  .t\CBV.dial :waste2b C'<  +38 h  .t\CS.seek 0ml C'k'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +6 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'd( +416 h ( M&{& h &ӷ M&i% h % .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &;'η +91 h 'η .tHand.open M&1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  #tda sampling at most 1000ml .DAfans.suppressMail "Started da in tube 2 at 12:30:29CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:29:43, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "da sampling at most 1000ml" #Loading sh1 puck from tube 2 into Collection Clamp C'n& h & M& % h %\  A(?% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ( ( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&& h &- M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &}( +165 h ( M&u& h & A(p& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A("& h &  .t\SE.seek 17.71 Pucks below Top Plate A(\ '* +86 h '* A(5& h &  M&& h & .t\Elbow.seek 2 M&M( +7 h ( M&&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M& '; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &s/ +150 h / ."downElevator"\SE.seek :down M&|& A(/'X h & M&% h %; .t\Forearm.seek :retract M&'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&=(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'A(W +416 h (W .tHand.open M&11 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1000ml w/bubble point of 24psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.reconfigure SSconfig .\SS.hold S)#.0X'4 +2609 h '4 C'a% h %4 S),% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'l @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X exit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:32:52, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:33:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:33:38, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'Q .t\CBV.dial CSV C'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 12:33:42CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:33:38, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV S)  .t\CBV.dial SSV +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&I& ."raiseStack"\SE.seek :upperLimit h &ӷ A(E'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&&& A(A'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %Y A(p& h & .t\Forearm.force -20 .\Forearm.seek 1 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&& h & A(q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*t  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & h & A('X M&q% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  'x  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'x& h &W C':% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'q  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'&  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'A  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV C')  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N 'X  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV C'Z .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'x% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV C'C  .t\CTV.dial CSV C'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  ' +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'\ .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Preparing to sleep until Thu Jan 01 06:00:00 +0100 1970 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00 #\Continuing at 06:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:35:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00" +155339 E ;3\\0\\0 C'|% P%p% h %4 M& % S) % h % % %\  M&n& h &- A(u& h & A(a% h %X C'& h & P%j& h & E ;3\\0\\0 .tGoodNews.suppressMail "At 06:00:00 began in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:35:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'K& h & .tHand.open M&1 + h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M&q/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +264 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &{' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'o& h &W C'% h %4 .t\CSV.dial CSR C'  +151 h  C'~% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'c'[ +462 h '[ .t\CSV.dial :waste C'/ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +2010 h '4 #t\Purging external Intake C'$& h &W C'7% h %4 .t\CSV.dial :flush C'3  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'G .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'(  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'z'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'!  +75 h #t\Flushing upper manifold C'k& h &W C'>% h %4 .t\CSV.dial :flush C'Y  +76 h  .t\CS.seek 5ml C'4'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'''i +415 h 'i ."connect"\CSV.dial CBV C'& .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'<'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'2& h &W C'A% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'0% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C')'0 +507 h '0 .t\CSV.dial :waste C'B +75 h .t\CS.seek 0ml C'I'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +6 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.seek CC M&\ ' +91 h ' .tHand.close M&F/ +150 h / .t\CC.seek :open C'N( +416 h ( M&S& h &ӷ M&M% h % .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &l(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&H% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #tWaiting for previous MFBPCR to complete... +61592 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*^  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*x  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*|'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*u% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ARV.dial :carrier F*?  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*V  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*{% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*/' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*7'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +107 h  ."MFBPCR"delay 7 seconds +700 F*1% h %< ."MFBPCR"\AS.seek 0.594ml F*G'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*( n +21 h n ."MFBPCR"delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.6ml F*!'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*k  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*Y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*4'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*<% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *J'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*)% h %n ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*U  +64 h  F*F% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*]  +32 h  ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +21 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*+  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ARV.dial :carrier F*9  +11 h  F*=% h %V ."MFBPCR"\AS.seek 0.5ml F*,'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*C n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*` I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*n' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*u  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +108 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*T  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*;% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*}  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*~'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*/  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*2% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*@'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*]  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*%  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*_ n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*H' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*Z I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*^'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*+  +107 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*P n +22 h n ."MFBPCR"delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.6ml F*e'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F**'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*= % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*C  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +87 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*d'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*|% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +31 h  ."MFBPCR"\ATV.dial :air3 F*y % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*?'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*5  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0113h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*c  +10 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*Y n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*&% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*s  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*v  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*4'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.65ml F*B' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*)  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*X% h % \\ ."MFBPCR"\AS.seek 0.326ml F*F'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0113h25ml.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*\ '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F**'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*,  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0115h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*j'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*q  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*y  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*; I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*t % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*}  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*?'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +22 h  ."MFBPCR"delay 7 seconds +700 F*V% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*.  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*$% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0115h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*/ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*7  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*(  +151 h F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *!' +423 h ' ."MFBPCR"\ATV.dial :ASV F*z n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*k% h % ."MFBPCR"\AS.seek 0.62ml F*Y'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*& n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *s'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*<'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *2' +424 h ' ."MFBPCR"\ATV.dial :air3 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*4% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*)' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*X% h % zG ."MFBPCR"\AS.seek 0.365ml F*B' +7161 h ' ."MFBPCR"\ATV.dial :waste F*R n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*U % +43 h % F*F% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h % ."MFBPCR"\AS.seek 0.579ml F*@' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*]'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*\ ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Z n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*H% h %V ."MFBPCR"\AS.seek 0.01ml F*+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*^% h % ."MFBPCR"\AS.seek 0.58ml F*P' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % ."MFBPCR"delay 3 seconds +300 F*f% h % q ."MFBPCR"\AS.seek 0.605ml F*m'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +21 h F*\\% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*i' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g  +21 h  ."MFBPCR"\AS.seek 0ml F*x'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *C' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*h n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b **'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*, n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*b'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*D n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *6' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*u  +42 h  F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *p': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*a  +64 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *j'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *y'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*K  +43 h  F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*r  +64 h  F*t% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*T n +65 h n F*}% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*V  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *?': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *o'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*~  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*{ % +43 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*c'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h %0 ."MFBPCR"\AS.seek 0.985ml F*3' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*G% h % ."MFBPCR"\AS.seek 0.97ml F*8'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*z I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *k'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *&'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *>'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *v' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *<' +9988 h ' ."MFBPCR"\ATV.dial AIV F*' I +43 h I ."MFBPCR"\AS.seek 0.02ml F*2' +9880 h ' ."MFBPCR"\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.seek 0ml F*4'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*B'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*F% h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +64 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *%'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*Q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*+'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*f% h %m ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*e  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b **'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*" % +108 h % ."MFBPCR"\AS.seek 0ml F*D'V +2547 h 'V #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 12:36:02CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:35:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*`% #tLoading sh1 puck from tube 2 into Collection Clamp h %V C'& h & M&b% h %\  A( % h %X A(6. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (|( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&n& h &- M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &a( +166 h ( M& & h & A(p& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &  .t\SE.seek 17.71 Pucks below Top Plate A(j'* +86 h '* A(q& h &  M&& h & .t\Elbow.seek 2 M&y( +6 h ( M&K& h & A(;& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &r/ +150 h / ."downElevator"\SE.seek :down M&t& h & A( 'X M&T% h %; .t\Forearm.seek :retract M&}'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' .t\CC.seek :closed C'5(W +416 h (W .tHand.open M&1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.reconfigure SSconfig .\SS.hold S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV S)v  .t\CBV.dial SSV +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C''% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Hd +100 C'v9 h 9Ld +100 C''9 h 9Pd +100 C'Y9 h 9Td +100 C's9 h 9Xd +100 C'A9 h 9(\\d +100 C'9 h 9@`d +100 C'29 h 9Wdd +100 C'49 h 9nhd +100 C'9 h 9ld +100 C'<9 h 9pd +100 C'/9 h 9td +100 C'09 h 9xd +100 C'J9 h 9|d +100 C'9 h 9d +100 C'9 h 9d +100 C'I9 h 9'd +100 C'R9 h 9>d +100 C'9 h 9Td +100 C'X9 h 9kd +100 C'U9 h 9d +100 C'B9 h 9d +100 C'9 h 9d +100 C'M9 h 9d +100 C'S9 h 9d +100 C'w9 h 9d +100 C'9 h 9 d +100 C')9 h 9 d +100 C'9 h 96d +100 C'@9 h 9Ld +100 C'_9 h 9bd +100 C'F9 h 9xd +100 C']9 h 9d +100 C'E9 h 9d +100 C'\ 9 h 9d +100 C' 9 h 9d +100 C'L9 h 9d +100 C'O9 h 9d +100 C'H9 h 9d +100 C'[9 h 9(d +100 C'Z9 h 9>d +100 C'Q9 h 9Td +100 C'%9 h 9id +100 C'^9 h 9d +100 C'9 h 9d +100 C'W9 h 9d +100 C'+9 h 9d +100 C'e9 h 9d +100 C'\\9 h 9d +100 C':9 h 9  d +100 C'f9 h 9 d +100 C'm9 h 9 *d +100 C'g9 h 9 ?d +100 C'x9 h 9 Td +100 C'P9 h 9 j d +100 C'i9 h 9 $d +100 C'99 h 9 (d +100 C'9 h 9 ,d +100 C'C9 h 9 0d +100 C'9 h 9 4d +100 C',9 h 9 8d +100 C'"9 h 9 % h %\  A(v% h %X A('. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (Y( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (s& h & M&A& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &2( +142 h ( M&4& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +90 h ' A(/& h & .t\SE.seek 1.71 Pucks below Top Plate A(0': +244 h ': M&J& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&I, +7 h , .t\Elbow.reconfigure TightElbow A(R- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(' +26 h ' M&X% h %Y A(U& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&B'υ +21 h 'υ M&& h & A(M& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &S' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(w' +36 h ' M&& h & M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&@(ӷ +143 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&E/ +150 h / .t\CC.seek :open C'\ ( +416 h ( A( & h & A(L& h & M&O& h &ӷ M&H% h % .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &Z( +142 h ( M&Q& h & A(%& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &^'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &W1 +150 h 1 M&+% h %Y A(e& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&\\'υ +20 h 'υ M&:& h & A(f& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &m' +23 h ' A(g% h % ."downElevator"\SE.seek :down A(x'X +100 (P% h %1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'C' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass #Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C',& h & .tHand.open M&"1 +2 h 1 M&*& h & M&=% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&h'η +91 h 'η .tHand.close M&u/ +150 h / M&n& h &- M&d% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'p(W +415 h (W C';& h &W .tHand.open M&`1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.reconfigure PSconfig .\PS.seek 1.5ml P%#A d`xb %T' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%r  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%a +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'  +76 h .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial :air P%j  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P%y +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%?  +38 h  .t\PS.seek 2.5ml P%N' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .t\PS.seek 0ml P%~' +2610 h ' #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +6 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M&$' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'1( +416 h ( M&7& h &ӷ M&% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &G(- +264 h (- M&3& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&1 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'|& h & M&k% h %\  A(z% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (v& h &  M&'& h &- M&s% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &A( +166 h ( M&& h & A(4& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(/& h &  .t\SE.seek 16.71 Pucks below Top Plate A('; +96 h '; A(& h &  M&J& h & .t\Elbow.seek 2 M&( +6 h ( M&R& h & A(X& h &  .t\Forearm.seek 2 + 300 counts M&U'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&M& A('X h & M&)% h %; .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.seek CC M&2(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &_' +91 h ' .t\CC.seek :closed C'F(W +416 h (W .tHand.open M&B1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'I  +150 h  .t\CSV.dial CTV C'E  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'5' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%O' +2610 h ' .t\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%Q  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%]  +150 h  .t\PS.seek :empty P%@' +2610 h ' .t\CBV.dial :waste1 C'W @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'+  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'e @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C':  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'f ` +18 h ` .t\CSV.dial :air C'g @ +38 h @ .t\CS.seek 10ml C'[' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'P'4 +2647 h '4 .t\CBV.dial :waste1 C'i @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'^& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(,% h %X A(". h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (*( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (=& h & M&\\& h &ӷ M&w% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &m( +142 h ( M&b& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &x' +90 h ' A(Y& h & .t\SE.seek 2.71 Pucks below Top Plate A(&') +234 h ') M&& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &n'Y +26 h 'Y .t\Elbow.hold M&d, +6 h , .t\Elbow.reconfigure TightElbow A(9- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(h' +27 h ' M&% h %Y A(l& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +20 h 'υ M&6& h & A(\ & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &;' +23 h ' .t\SE.seek 4.04 Pucks below Top Plate A(`' +36 h ' M&K& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&a/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(& h & M&& h &ӷ M&j% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +142 h ( M&}& h & A(?& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&{, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &N1 +150 h 1 M& % h %Y A(o& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&V'υ +20 h 'υ M&& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &q' +22 h ' A(p% h % ."downElevator"\SE.seek :down A(7'X +100 (% h %  M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%.% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%c +37 h .t\PRV.dial :air P%(  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%|  +38 h .t\CBV.dial AIV C'k  +37 h .t\PRV.dial between CBV and air P%z  +19 h  .t\PRV.dial :air P%  +19 h  P%v% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %'' +250 h ' .t\PRV.dial :waste2 P%s @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %8' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&41 +2 h 1 M&/& h & M&G% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.seek :garage M&X'η +91 h 'η .tHand.close M&!/ +150 h / M&& h &- M&M% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &A(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'U& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  C'1& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%B @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%I  +75 h  .tdelay 2 seconds +200 P%E% h % .t\PS.seek 1.499ml P%_' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%>' +1094 h ' .t\PSV.dial :air P%C  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%5 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%L  +38 h  .tdelay 2 seconds +200 P%~% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %H'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%Q  +75 h .tdelay 2 seconds +200 P%%% h %r .t\PS.seek 0.475ml P%]' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%O  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%W'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%+  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%e  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %:' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%f +76 h .t\PTV.dial :PRV,avoiding: :puck P%g @ +75 h @ .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%[' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%i +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%^  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%, +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%"  +76 h P%=% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %\\' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'w & +50 h & .t\AWV.select :waste C' +6 P%b% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'Y { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %2' +1313 h ' .t\PSV.dial :air P%S  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P%u +38 h .t\PS.seek 0ml P%9' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'l& F*6 @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*\  P%K +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%x' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*m I +21 h I F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.3ml F*#,,'Td20 b *n': +174 .t\PSV.dial :waste1 P%#  +37 h  .t\PS.seek 0ml P%r' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%h' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%; +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +1480 h ': ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +43 h  F*t% h %: ."MFBPCR"\AS.seek 0.33ml F*F'ї +87 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +419 h 'ї ."MFBPCR"\ATV.dial :waste2 F*j % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +1156 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%?  +38 h  .t\PS.seek 0ml P%T' +2 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b * ' +1430 h ' ."MFBPCR"delay 3 seconds +144 h ' .t\PSV.dial PSR P%o +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +112 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*p% h % ."MFBPCR"\AS.seek 0.469ml F* ' +2455 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%$ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%V' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%`  +37 h  .t\PS.seek 0ml P%' +635 h ' ."MFBPCR"delay 5 seconds +500 F*.9 h 9j #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*q n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*7% h % ."MFBPCR"\AS.seek 0.041ml F**'% +441 h ' .t\PSV.dial PSR P%@ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%c' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%( +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%|' +2610 h ' .t\PSV.dial PSR P%k +113 h P%z% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +557 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +33 h ' .t\PSV.dial PSR P%s +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%'' +123 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2487 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %/'k +2678 h 'k .t\PSV.dial :waste1 P%G  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %J' +106 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*R I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *{'V +954 h ' .t\PSV.dial :air P%!  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%M +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %3' +5107 h ' .t\PSV.dial :air P%X  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1222 h 'V ."MFBPCR"\ATV.dial :air2 F*0  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *)'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*U% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +275 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %1' +514 h ' ."MFBPCR"\ATV.dial :waste F*B n +128 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*I% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *E' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*8  +43 h F*_% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *C'_ +2775 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'>  +38 h  C'5% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'L'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'~' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'Q  +38 h  +113  .t\CBV.dial :waste1 C'% @ +37 h @ .t\CS.seek 0ml C'H'4 +1811 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*O  +22 h ."MFBPCR"delay 3 seconds +300 F*]% h %_ ."MFBPCR"\AS.seek 0.434ml F*+'ݏ +214 h '4 .t\CSV.dial CSR C'e  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'f'P +267 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*g  +22 h F*:% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +324 h '& ."MFBPCR"delay 3 seconds +203 h 'P .tdelay 5 seconds +97 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*i% h %& ."MFBPCR"\AS.seek 0.092ml F*['&0 +296 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'^' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C','4 +2647 h '4 C'"& h &W #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.seek CC M&\\' +91 h ' .tHand.close M&Y/ +150 h / .t\CC.seek :open C' ( +416 h ( M&S& h &ӷ M&2% h % .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\ 'η +90 h 'η .tHand.open M&K1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'm& h & M&&% h %\  A(#% h %X A(D. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (x( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&r& h &- M&;% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &h( +166 h ( M&a& h & A(t& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A(n'L +107 h 'L A(d& h &  M&j& h & ."WCR"\Elbow.seek 2 M&F( +6 h ( M&?& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&N'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +44 h '&0 ."MFBPCR"delay 3 seconds +106 h / ."downElevator"\SE.seek :down M&}& h & M& % A(o'X h %; ."WCR"\Forearm.seek :retract M&p'\ +91 h '\  ."WCR"\Elbow.seek CC M&T(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +87 ."MFBPCR"\ATV.dial :waste2 F* % +40 h (ӷ M&y& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +3 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +88 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&`1 +55 h 'R ."MFBPCR"\ATV.dial :air2 F*V  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *.' +73 h 1 M&q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +6 h + S)@% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'(  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*5 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*L  +87 h F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *Q'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*% I +43 h I F*~% h %N ."MFBPCR"\AS.seek 0.109ml F*O'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*] n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*e  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *W% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *C' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *H% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *g' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*:% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *+' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F* % h % ."MFBPCR"\AS.seek 0.279ml F*i'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Z n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*^% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *"'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*= I +21 h I F*w% h %v ."MFBPCR"\AS.seek 0.059ml F*b'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*Y % +21 h % F*A% h %H ."MFBPCR"\AS.seek 0.069ml F*<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*S% h % ."MFBPCR"\AS.seek 0.769ml F*2' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*l' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*u I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *m' +4416 h ' ."MFBPCR"\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.seek :empty F*#'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*D % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.579ml F*a' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*j% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*9  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*h % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*y' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F* % h %  ."MFBPCR"\AS.seek 0.09ml F*N' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*F I +86 h I F*o% h % ."MFBPCR"\AS.seek 0.12ml F*p' +625 h ' ."MFBPCR"\ATV.dial :air3 F*[ % +21 h % F* % h % ."MFBPCR"\AS.seek 0.13ml F*V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*q% h % ."MFBPCR"\AS.seek 0.23ml F*@' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*('X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*x% h %X ."MFBPCR"\AS.seek 0.24ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*z n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*k  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*c @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F** I +86 h I ."MFBPCR"\AS.seek 0ml F*4'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*.'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*G  +43 h  #"MFBPCR"\Swish #1 of 2 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *R'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*!'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*J% h %(A ."MFBPCR"\AS.seek 0.6ml F*M'\  +1032 h '\  F*/% h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*0% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*{ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*X  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0122h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*U  +64 h  ."MFBPCR"\ARV.dial :carrier F*)  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +21 h ."MFBPCR"delay 2 seconds +200 F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*E  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*>' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*L  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*P' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*% % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +108 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*O n +21 h n ."MFBPCR"delay 2 seconds +200 F*H% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*:  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*+  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*i  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*^'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*= % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254676 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'w  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'" @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'A @ +75 h @ +50 S)b% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'S% h % ."WCR"\CSV.dial between air and CTV C'< ` +19 h ` ."WCR"\CS.seek 7.288ml C'\\' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'2  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'K  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'l' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'u  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'&% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'D' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'm @ +75 h @ +50 S)#% h % ."WCR"\CBV.dial between CSV and waste2b C';  +95 h  C'\ % h % ."WCR"\CSV.dial between air and CTV C'r ` +56 h ` ."WCR"\CS.seek 7.288ml C't' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'a  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'j  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'9  +37 h  C'?% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '}' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'h @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'y' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'F  'n  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'o'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'N  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '[' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'p& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&`% h %\  A(q% h %X A(V. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&@& h &ӷ M&$% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &x( +143 h ( M&(& h & A(z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A( & h & ."WCR"\SE.seek 3.71 Pucks below Top Plate A(v' +224 h ' M&|& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &*'Y +25 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(4- h -+ M&$&.,(\ #b ."WCR"\SE.seek 4.04 Pucks below Top Plate A(G' +27 h ' M&s% h %Y A(.& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&R& h & A(J& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &!' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(/' +36 h ' M&M& h & M&0% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +90 h '\  ."WCR"\Elbow.seek CC M&{(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ ."WCR"\Forearm.seek CC M&U' +91 h ' ."WCR"Hand.close M&)/ +150 h / ."WCR"\CC.seek :open C''( +416 h ( A(& h & A(& h & M&& h &ӷ M&B% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&8& h & A(_& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &I'Y +93 h 'Y ."WCR"\Elbow.hold M&E, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &51 +150 h 1 M&% h %Y A(L& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&>& h & A(%& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(P% h % ."downElevator"\SE.seek :down A(]'X +100 (e% h % M&Q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&W1 +2 A(6 h 1 M&:& h & M&g% h %\  .t\Elbow.seek FlushPuck::Garage M&C(- +166 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&i'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&f% h %η .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +264 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &A' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'& h &W .tHand.open M&S1 +150 h 1 M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'2& h &W C'% h % .t\CSV.dial CSR C'\\  +38 h  C'K% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'u% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C'& +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'm% h %4 .t\CSV.dial :flush C'#  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ';'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'r% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'a  +38 h  +113  .t\CBV.dial :waste1 C't @ +75 h @ .t\CS.seek 0ml C'j'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'd& h &W C'% h %4 .t\CSV.dial :flush C'9  +75 h  .t\CS.seek 5ml C'?'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C' & h &W C'}% h %4 .t\CSV.dial :flush C'n  +75 h  .t\CS.seek 5ml C'F'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +37 h @ C'N% h %p .t\CS.seek 7ml C'o'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'p  +38 h +75 .t\CTV.dial :air C'`  +151 h .t\CS.seek 0ml C'q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'@'4 +507 h '4 C'$& h &W C'(% h %4 .t\CSV.dial :flush C'z  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +37 h @ C'7% h %0 .t\CS.seek 5ml C'|'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +506 h '0 .t\CSV.dial :waste C's +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'R& h &W C'J% h %4 .t\CSV.dial CSR C'M  +151 h  C'0% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'k'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'x' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'v +113 h .t\CTV.dial :intake C'*  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'X  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'G  +113 h  C'!% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +709 h ' ."connect"\CSV.dial CTV C'3  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'B% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '8'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '_' +710 h ' ."connect"\CSV.dial CTV C'U  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'{  +75 h  .t\CS.seek 0ml C'5'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'L% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '>'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '%' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P '  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'e'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +507 h '0 .t\CSV.dial :waste C'H +37 h .t\CS.seek 0ml C'W'4 +507 h '4 #t\Flushing upper manifold C':& h &W C'g% h %4 .t\CSV.dial :flush C'I  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'+% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'~ +113 h .t\CTV.dial :air .\CS.seek 0ml C']'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'E +37 h .t\CS.seek 0ml C''4 +507 h '4 C' & h &W C'f% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C'i'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C',% h %0 .t\CS.seek 5ml C'C'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'='4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'S'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'<& h &W C'2% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'A% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CTV C'u  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'& @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'D& h &W C'm% h %4 .t\CSV.dial :flush C'#  +75 h  .t\CS.seek 5ml C'l'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'r% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'a  +75 h  .t\CS.seek 0ml C't'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'd% h %4 .t\CSV.dial CSR C'  +38 h  C'9% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'j'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +415 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'?  +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C' ' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'h  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +606 h '4 .t\CSV.dial CSR C'}  +113 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'y' +1372 h ' .t\CSV.dial CBV C'N +113 h .t\CS.seek 0ml C'F'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&p& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&$& h &ӷ M&(% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&|1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'4& h & .tHand.open M&R1 +2 h 1 M&J& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &M(- +7 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.seek :garage M&k'η +90 h 'η .tHand.close M&v/ +150 h / M&*& h &- M&x% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'3& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'B% h %4 .t\CSV.dial CSR C'  +113 h  C'8% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'U'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '{% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'5& h &W C'>% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'%  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'e'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'H  +75 h #t\Flushing upper manifold C'Q& h &W C':% h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C'I'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +37 h @ C'/% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +507 h '0 .t\CSV.dial :waste C'] +37 h .t\CS.seek 0ml C'E'4 +507 h '4 C'& h &W C' % h %4 .t\CSV.dial :flush C'f  +38 h  .t\CS.seek 3ml C'O'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'"% h %0 .t\CS.seek 5ml C','p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'= +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&'& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +7 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'A( +416 h ( M&\\& h &ӷ M&u% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +264 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  #t\Completed [mbaPhase] begun 06:00:00 #\Extending 18tundeploy until completion of MFBPCR @CET83659.02 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0122h25ml.pcr F*r% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*a % +86 h % F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*}% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +21 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*7'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*|% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0200h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*k  +43 h  ."MFBPCR"\ARV.dial :carrier F*G  +11 h  F*!% h %V ."MFBPCR"\AS.seek 0.5ml F*M'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*8  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*{  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*_  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*>'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*% n +22 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*e  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*/% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*+ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0200h25ml.pcr F*~% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +21 h % ."MFBPCR"delay 3 seconds +300 F*,% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +21 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +31 h  ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +21 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*;% h %m ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0202h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*w  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*$ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*Y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*T n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*|  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*R'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*J n +21 h n ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*.'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.65ml F**' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*x  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*!  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*s % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0202h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*F% h %n ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *L'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0205h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ARV.dial :carrier F*'  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\\ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +21 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *['Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*m% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*r I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*6' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*a % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +107 h  ."MFBPCR"delay 7 seconds +700 F*l% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0205h25ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*(% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +32 h  ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*x% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F**% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*G'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*V  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0207h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F*L% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*e  +22 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *:'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*g n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*/' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*c  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.53ml F*+' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Z  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*I'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*E'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*f% h %\  ."MFBPCR"\AS.seek 0.6ml F*O'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*,  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*i  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*2% h % \\ ."MFBPCR"\AS.seek 0.326ml F*K'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0207h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*\\  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*S'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*^% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*a  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +65 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +21 h F*N% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0209h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*4'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*v  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*k I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F**% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*s % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*!' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*X  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*G'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*F  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*L % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0209h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*>  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*%  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *H' +424 h ' ."MFBPCR"\ATV.dial :ASV F*e n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*W  +43 h  F*Q% h % ."MFBPCR"\AS.seek 0.62ml F*g'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *c'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*~'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*/ % +43 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h % H ."MFBPCR"\AS.seek 0.735ml F*,' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*O% h % Ű ."MFBPCR"\AS.seek 0.72ml F*C' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*i n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*2% h % zG ."MFBPCR"\AS.seek 0.365ml F*=' +7162 h ' ."MFBPCR"\ATV.dial :waste F*' n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*K% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*u% h % ."MFBPCR"\AS.seek 0.579ml F*)' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %  ."MFBPCR"\AS.seek 0.604ml F*D'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*m% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*S' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*r n +85 h n ."MFBPCR"\ASV.dial :wasteR F*^  +22 h  ."MFBPCR"\AS.seek 0ml F*['V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*6 % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*d' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*?% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*h'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*n  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *y' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*N n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +202 h 'R ."MFBPCR"\SPE.coast F*w  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*`'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*$  +43 h  F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *o' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*T I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*|  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *q': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*7% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *4'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*J n +64 h n F*z% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *0' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*v I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *.'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*R  +43 h  F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b **': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *M'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*@ n +65 h n F*X% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *3' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*! I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *V'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *G': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*B n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*8  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *{' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*U% h % ."MFBPCR"\AS.seek 0.96ml F*_'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*P'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*e I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Q'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *:'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +43 h  F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Z' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *~' +9988 h ' ."MFBPCR"\ATV.dial AIV F*+ I +42 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*I'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*]% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*2'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F*='n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*\\% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +31 h  ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*d'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *?'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *y'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*w n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*p % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tundeploy assays .GoodNews.suppressMail "{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@12:36:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tundeploy assays" #\Mission runtime was 4:42.559732 #-> ls !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message exit #m\EXIT @1535625506.56CEST @1535632513.40CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:129: syntax error, unexpected $end, expecting kEND #-> execute "18tundeploy.rd" !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"ANo '18tundeploy.rd' script found on /home/esp/esp2/mission:. #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:129: syntax error, unexpected $end, expecting kEND #-> exit #m\EXIT @1535632690.99CEST @1535632759.07CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi,!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:130: syntax error, unexpected $end, expecting kEND #-> exit #m\EXIT @1535632797.93CEST @1535632861.93CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:130: syntax error, unexpected $end, expecting kEND #-> exit #m\EXIT @1535632894.13CEST @1535632997.36CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:129: syntax error, unexpected $end, expecting kEND #-> exit #m\EXIT @1535633031.47CEST @1535633530.73CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:52:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:53:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:53:38, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract .\Forearm.hold M&w+ +6 h + !to:Puck::Warning:bt["7/home/esp/esp2/utils/puckmoves.rb:223:in `giveup!'"6/home/esp/esp2/utils/puckmoves.rb:767:in `garage'"6/home/esp/esp2/utils/puckmoves.rb:785:in `loadAt'"=/home/esp/esp2/utils/puckmoves.rb:898:in `loadFlushPuck'">/home/esp/esp2/protocol/utilities.rb:75:in `initialPurge'";/home/esp/esp2/mission/18tundeploy.rb:38:in `mbaPhase'"./home/esp/esp2/mission/18tundeploy.rb:107"7/home/esp/esp2/utils/commskel.rb:81:in `runAction'"//home/esp/esp2/utils/mission.rb:71:in `at'"./home/esp/esp2/mission/18tundeploy.rb:106"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"./home/esp/esp2/mission/18tundeploy.rb:104"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"BRetaining claim on Arm because Hand is likely holding a puck! !o:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"%Forearm seek to retract Aborted!:@checkpoint: omitted #-> exit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"(Exiting with Hand holding FlushPuck #m\EXIT @1535633637.22CEST @1535634065.30CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:130: syntax error, unexpected $end, expecting kEND #-> exit #m\EXIT @1535634101.45CEST @1535634217.78CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:03:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:04:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:04:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 + .t\CS.hold C'f+ h '4 +7 +4 !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg",Collection Syringe seek to 3ml Aborted!:@checkpoint: omitted #-> exit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"4Exiting with Collection Clamp holding FlushPuck #m\EXIT @1535634261.10CEST @1535634325.71CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"]/home/esp/esp2/mission/18tundeploy.rb:131: syntax error, unexpected $end, expecting kEND #-> exit #m\EXIT @1535634357.24CEST @1535634413.79CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "10tundeploy.rb" !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"ANo '10tundeploy.rb' script found on /home/esp/esp2/mission:. #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:07:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:07:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:07:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:07:34CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:07:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X exit .\AS.stop !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:13:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:14:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ F*\ 9\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:14:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q  'R h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:14:14CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:14:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'& ."readyMFB"\AFV.select :holdingCoil F* h & M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'Q9 h 9|d +100 C'R9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b M&I& A( & h &ӷ & .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(E'Z .t\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(Q& h & M&R& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&&& h & A(A'X M&B% h %; !to:Puck::Warning:bt["7/home/esp/esp2/utils/puckmoves.rb:223:in `giveup!'"4/home/esp/esp2/utils/puckmoves.rb:349:in `load'"8/home/esp/esp2/utils/puckmoves.rb:882:in `loadPuck'"?/home/esp/esp2/protocol/PRVprocess.rb:302:in `transferPRV'"//home/esp/esp2/protocol/MFB.rb:44:in `mfb'":/home/esp/esp2/protocol/utilities.rb:67:in `protocol'"//home/esp/esp2/protocol/MFB.rb:41:in `mfb'";/home/esp/esp2/mission/18tundeploy.rb:39:in `mbaPhase'"./home/esp/esp2/mission/18tundeploy.rb:107"7/home/esp/esp2/utils/commskel.rb:81:in `runAction'"//home/esp/esp2/utils/mission.rb:71:in `at'"./home/esp/esp2/mission/18tundeploy.rb:106"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"./home/esp/esp2/mission/18tundeploy.rb:104"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"BRetaining claim on Arm because Hand is likely holding a puck! !o:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"User Abort!!:@checkpoint: omitted #-> exit .\SE.stop !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"2Exiting with Hand holding Puck.from 2, :lyfil #m\EXIT @1535634881.46CEST @1535634905.91CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 03:00:57CET01-Jan-70 .delayUntil "03:00:57.00" +725699 !o:Storage::Warning:bt["8/home/esp/esp2/utils/storage.rb:415:in `readPucks!'"2/home/esp/esp2/utils/storage.rb:414:in `each'"8/home/esp/esp2/utils/storage.rb:414:in `readPucks!'"6/home/esp/esp2/utils/storage.rb:597:in `restore!'"-/home/esp/esp2/lib/esp.rb:127:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"#Hand.holds Puck.from 2, :lyfil #\ESP.ready! will restore puck positions #-> exit #m\EXIT @1535634920.54CEST @1535634932.47CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> delayUntil '08:00 30-Aug-18' .delayUntil "08:00:00.00CEST30-Aug-18" @CEST1535608800.00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:15:40, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 08:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:17:26, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 08:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:17:30, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:17:34CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:17:30, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&% F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV S)  .t\CBV.dial SSV +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&I& ."raiseStack"\SE.seek :upperLimit h &ӷ A(E'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A(!% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ($& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down M&A& A(&'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %Y A(p& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%!  +19 h  .t\PRV.dial :air P%2  +19 h  P%$% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&A% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&&1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*t  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%!% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%2' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*&' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M& & h & M&x% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*! n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *2'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*A'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug3011h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  'q  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A(!& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A($'K +254 h 'K M&/& h & A(2& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(7- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&A& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(1& h & A(& h & M&&& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C':% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'c .t\CBV.dial CSV C'{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'!  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'A  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV C'$ .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'&  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X  'N +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'q% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  ' +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&!'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &A(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&$/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'&& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'H .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 08:00:26 #\Preparing to sleep until Fri Aug 31 06:00:00 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST31-Aug-18 #\Continuing at 06:00CEST31-Aug-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:18:57, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST31-Aug-18" +235134 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118aug3011h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18aug3013h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*A'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*X  +107 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18aug3013h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*^  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18aug3015h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*/  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*2  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*('\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*4 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18aug3015h25ml.pcr F*A% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*<  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *&'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*B  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*L % +86 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18aug3018h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +10 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*d n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*| I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18aug3018h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*$  +31 h  ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +21 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug3020h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*l n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*s  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +22 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218aug3020h25ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *;' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18aug3022h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*$  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *z'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*4 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*<  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +22 h  ."MFBPCR"delay 7 seconds +700 F*1% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 @CEST1535669496.94 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18aug3022h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*B @@T ."MFBPCR"\ASV.dial :SPEdelivery F*%  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*F  +151 h F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *S' +423 h ' ."MFBPCR"\ATV.dial :ASV F*L n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*M% h % ."MFBPCR"\AS.seek 0.62ml F*l'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *s'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*f' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % H ."MFBPCR"\AS.seek 0.735ml F*e' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +21 h F*W% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*q% h % zG ."MFBPCR"\AS.seek 0.365ml F*m' +7161 h ' ."MFBPCR"\ATV.dial :waste F** n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*C% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +21 h F*`% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*"' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*| n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*b'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *n'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*u' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*;'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*? n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *r'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +202 h 'R ."MFBPCR"\SPE.coast F*V  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +42 h  F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *~': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*2  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *7'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*8 n +65 h n F*!% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*$ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *k': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*z  +64 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *A'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Y n +65 h n F*<% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*1  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *&'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*J n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*0 % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*)% h % ."MFBPCR"\AS.seek 0.96ml F*I'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %0 ."MFBPCR"\AS.seek 0.985ml F*X' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.97ml F*N'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*L I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *M'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Z' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*[% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*+  +21 h ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*\\% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *r'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*{ % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +1413369 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '@ h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)/% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'c& S)#.0X  M&z% P%4  h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &k'\ h ."rdySampler"\CSV.dial :waste1 C'Y h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)< h  S)A  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %'' +20 h '\  ."closeHand"Hand.close .t\Elbow.jog 1500 M&1/ &* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:19:52, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&X1 +150 h 1 M&L& h &_ M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &](- +166 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &H'η +91 h 'η .tHand.close M&l/ +150 h / M&s& h &- M& % h %η .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'P% h %4 .t\CSV.dial CSR C'e  +150 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C'q +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ':'4 +2009 h '4 #t\Purging external Intake C'& h &W C'm% h %4 .t\CSV.dial :flush C',  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h '9  h +75 .t\CTV.dial :intake C'D  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'=  +75 h #t\Flushing upper manifold C'd& h &W C'`% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'E'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'b% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'p +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'n& h &W C'K% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'a'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'u% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&w& h &ӷ M&{% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&-1 +150 h 1 M&#% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:19:57CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:19:52, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'B& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(@% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&/% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &c( +6 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F*2 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +160 h ( M&~& h & A(8& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &!' +89 h ' A(7& h &  .t\SE.seek 17.71 Pucks below Top Plate A($'* +87 h '* A(G& h &  M&(& h & .t\Elbow.seek 2 M&3( +6 h ( M&& h & A(z& h &  .t\Forearm.seek 2 + 300 counts M&>'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down M&4& A(<'X h & M&A% h %; .t\Forearm.seek :retract M&1'\ +87 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .t\Elbow.seek CC M&J(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'U(W +416 h (W .tHand.open M&%1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'0& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)\ % h % C''4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9(d +100 C'$9 h 9 ,d +100 C'<9 h 9$0d +100 C'39 h 9<4d +100 C'19 h 9S8d +100 C'V9 h 9kd +100 C']9 h 9Td +100 C'_9 h 9kd +100 C'F9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C's9 h 9d +100 C'N9 h 9d +100 C'i9 h 9d +100 C'P9 h 9 d +100 C'e9 h 9 d +100 C'Z9 h 96d +100 C'Q9 h 9Ld +100 C')9 h 9bd +100 C'g9 h 9xd +100 C'W9 h 9d +100 C'q9 h 9d +100 C'\\9 h 9d +100 C'*9 h 9d +100 C'm9 h 9d +100 C',9 h 9d +100 C'99 h 9d +100 C'9 h 9(d +100 C'D9 h 9>d +100 C'+9 h 9Td +100 C'h9 h 9id +100 C':9 h 9d +100 C'C9 h 9d +100 C'd9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'=9 h 9  d +100 C'b9 h 9 d +100 C'E9 h 9 *d +100 C'|9 h 9 ?d +100 C'69 h 9 Td +100 C'O9 h 9 j d +100 C'p9 h 9 $d +100 C' 9 h 9 (d +100 C'n9 h 9 ,d +100 C'K9 h 9 0d +100 C' 9 h 9 4d +100 C'9 h 9 8d +100 C'u9 h 9   +37 h C'$% h %p .t\CS.seek 7ml C'<'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'3 @ +38 h @ .t\CS.seek 10ml C'1' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'V ` +132 h ` #tLysing Complete C'%& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(0% h %X A(\ . h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (L( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&j% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&'& h & A(M& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(S& h & .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': M&l& h & A(X& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b & 'Y +26 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(H- h -+: M&$&.,(\ #b .t\SE.seek 2.04 Pucks below Top Plate A(' +27 h ' M&]% h %Y A(_& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &F'υ +20 h 'υ M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(N' +37 h ' M&i& h & M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +89 h '\  .t\Elbow.seek CC M&Z(ӷ +143 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'q( +415 h ( A(\\& h & A(*& h & M&m& h &ӷ M&,% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&D& h & A(+& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &h'Y +92 h 'Y .t\Elbow.hold M&:, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&d% h %Y A(`& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&"& h & A(=& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &b' +22 h ' A(E% h % ."downElevator"\SE.seek :down A(|'X +100 (6% h %1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'n' +6 h ' .t\CSV.dial between air and CTV C'K ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&u1 +2 h 1 M&a& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &{' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'[& h &W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%? +151 h P%-% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%r' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%#' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%B' +2610 h ' ."connect"\PRV.dial CBV P%@  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%o  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :mixloop P%~ +38 h .t\PS.seek 0ml P%8' +2609 h ' .t\PSV.dial :air P%2  +38 h  .t\PS.seek 2.5ml P%7' +2610 h ' .t\PSV.dial :mixloop P%( +37 h .t\PS.seek 0ml P%G' +2610 h ' #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +7 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&4' +90 h ' .tHand.close M&A/ +150 h / .t\CC.seek :open C'z( +416 h ( M&c& h &ӷ M&!% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +265 h (- M&>& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&31 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&%% h %\  A(k% h %X A(0. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (\ ( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&j% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &'( +166 h ( M&M& h & A(S& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(& h &  .t\SE.seek 16.71 Pucks below Top Plate A(l'; +96 h '; A(X& h &  M&H& h & .t\Elbow.seek 2 M&]( +6 h ( M&_& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &i/ +150 h / ."downElevator"\SE.seek :down M&& A(P'X h & M&% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'e  +150 h  .t\CSV.dial CTV C'U  +19 h  .t\CS.seek 5ml C'\\'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'*' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'm'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%,  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P%+ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Z +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%C  +151 h  .t\PS.seek :empty P%d' +2609 h ' .t\CBV.dial :waste1 C'` @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'"  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'= @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'9 ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'6' +2648 h ' .t\CSV.dial CTV C'O  +37 h  .t\CS.seek 0ml C' '4 +2647 h '4 .t\CBV.dial :waste1 C'h @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'K  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(u% h %X A(a. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&N& h &ӷ M&b% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +143 h ( M&x& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &|' +89 h ' A(1& h & .t\SE.seek 2.71 Pucks below Top Plate A(:') +235 h ') M&& h & A( & h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&p, +7 h , .t\Elbow.reconfigure TightElbow A(;- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(f' +27 h ' M&w% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&^'υ +21 h 'υ M&q& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(' +36 h ' M&?& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(& h & M&B& h &ӷ M&o% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &~( +142 h ( M&/& h & A(2& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &8'Y +93 h 'Y .t\Elbow.hold M&(, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &71 +150 h 1 M& % h %Y A(.& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &Y'υ +21 h 'υ M&A& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(c'X +100 (!% h %  M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%$  +2 A(6 +111 h  P%5% h % .t\PS.seek 0.499ml P%3' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'%  +38 h .t\PRV.dial between CBV and air P%k  +18 h  .t\PRV.dial :air P%0  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%j @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %R' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&S1 +2 h 1 M&& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&X(- +166 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*Q  +43 h  F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +170 h ' .t\PSV.dial :mixloop P%P +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %z' +618 h ' ."MFBPCR"\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.seek :empty F*)'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*e% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *U' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*R  +43 h F*F% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *n'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'\  +37 h  C'*% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1625 h ' ."connect"\CSV.dial CTV C'\\  .t\CTV.dial CSV C'g  +38 h  +113  .t\CBV.dial :waste1 C'Z @ +38 h @ .t\CS.seek 0ml C'+'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*D  +22 h ."MFBPCR"delay 3 seconds +300 F*C% h %_ ."MFBPCR"\AS.seek 0.434ml F*"'ݏ +110 h '4 .t\CSV.dial CSR C'=  +37 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'9'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*E% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*O'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*m n +107 h n F*h% h %& ."MFBPCR"\AS.seek 0.092ml F*6'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'u'4 +2647 h '4 C'a& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&N' +91 h ' .tHand.close M&1/ +150 h / .t\CC.seek :open C'L( +416 h ( M&W& h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &['η +91 h 'η .tHand.open M&?1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'y& h & M&:% h %\  A(r% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (|( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (^& h &  M&& h &- M&}% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &f( +166 h ( M&@& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &{' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A(p& h &  M&o& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&2& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&7'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &B/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down M&/& A(.'X h & M&% h %; ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.seek CC M&#(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &A' +64 ."MFBPCR"\ATV.dial :waste2 F*4 % +27 h ' ."WCR"\CC.seek :closed C'8(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +400 h (W ."WCR"Hand.open M&1 +150 h 1 M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*t  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *c' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)d% h % C'$& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % C'Q& h &W E ;3\\0\\0 ."WCR"\CTV.dial :intake ."connect"\SSV.dial CBV S)X  ."WCR"\CBV.dial SSV +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'(% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*Q% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *X' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*( % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug3107h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*P  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*U  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F**% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*,  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Z % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +107 h  ."MFBPCR"delay 7 seconds +700 F*=% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*E  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 F*O  +65 h ."MFBPCR"\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254571 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'b  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'a @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'' @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'l  +95 h  C'W% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'N' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'w  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'-  +37 h  C':% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'v' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S)r% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'[% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '@  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'p'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'o  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C';  +38 h  C'2% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'f @ +75 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'~% h % ."WCR"\CSV.dial between air and CTV C'7 ` +57 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '4  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '.'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'6  +150 h C'T& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&Y% h %\  A(t% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ($( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&d& h &ӷ M&!% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &|( +142 h ( M&A& h & A(k& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(& h & ."WCR"\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' M&V& h & A(0& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y ."WCR"\Elbow.hold M&3, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(S- M&$&.,(\ #b h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(&' +27 h ' M&j% h %Y A(c& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&M'υ +21 h 'υ M&_& h & A(H& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +37 h ' M&& h & M&Q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +89 h '\  ."WCR"\Elbow.seek CC M&((ӷ +142 h (ӷ M&X& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&P/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(i& h & A(J& h & M&]& h &ӷ M&s% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&R& h & A(F& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &e'Y +92 h 'Y ."WCR"\Elbow.hold M&U, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &*1 +150 h 1 M&% h %Y A(,& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&\ & h & A(Z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &G' +22 h ' A( % h % ."downElevator"\SE.seek :down A(C'X +100 (=% h % M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +90 h '\  +21 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&`1 +2 A(6 h 1 M&E& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&n(- +166 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&h'η +91 h 'η .tHand.close M&K/ +150 h / M&m& h &- M&9% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +265 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'x(W +415 h (W C'l& h &W .tHand.open M&W1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'w& h &W C' % h % .t\CSV.dial CSR C'N  +38 h  C'?% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '-'[ +463 h '[ .t\CSV.dial :waste C': +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'v& h &W C'y% h %4 .t\CSV.dial :flush C'r  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CTV C'@  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'p& h &W C'% h %4 .t\CSV.dial :flush C';  +76 h  .t\CS.seek 5ml C'2'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'B% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'~'4 +765 h '4 #t\Flushing upper manifold C'7& h &W C'/% h %4 .t\CSV.dial :flush C'4  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6 'T  +37 h +76 .t\CTV.dial :air C'Y  +150 h .t\CS.seek 0ml C't'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'd'4 +507 h '4 C'!& h &W C'A% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +38 h @ C'5% h %0 .t\CS.seek 5ml C'V'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'0'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C'c'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'_& h &W C'H% h %4 .t\CSV.dial CSR C'  +151 h  C'Q% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'%'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'|' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'X' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'&  +113 h  C'<% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'3'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +710 h ' ."connect"\CSV.dial CTV C'>  .t\CTV.dial CSV C'8  +37 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C'J'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C']  +38 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'R'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'(  +75 h  .t\CS.seek 0ml C'*'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C',% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\ 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'='4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'`'4 +507 h '4 #t\Flushing upper manifold C'E& h &W C'% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'"% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'$'0 +507 h '0 .t\CSV.dial :waste C'U +38 h .t\CS.seek 0ml C'K'4 +506 h '4 C'm& h &W C'9% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 3ml C'h'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'l +75 h .t\CS.seek 0ml C'W'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'w% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'N'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C''% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C': @ +38 h @ .t\CS.seek 0ml C'-'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'v& h &W C'y% h %4 .t\CSV.dial :flush C'r  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'^& h &W C'p% h %4 .t\CSV.dial CSR C'  +37 h  C';% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'o'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'B .t\CBV.dial CSV C'2  +37 h +76 .t\CTV.dial :intake C'{  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'f  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '7'4 +607 h '4 .t\CSV.dial CSR C'/  +112 h  C'4% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'~'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1373 h ' .t\CSV.dial CBV C' +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&!& h &ӷ M&A% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &d(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&V1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&_1 +2 h 1 M&H& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'>& h &W .tHand.open M&81 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C's% h %4 .t\CSV.dial CSR C'J  +113 h  C'R% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '(% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'F'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Purging external Intake C'*& h &W C'\ % h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'Z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'='4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'+  +75 h #t\Flushing upper manifold C'g& h &W C'E% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'e'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'% h %p .t\CS.seek 7ml C'"'i +416 h 'i ."connect"\CSV.dial CBV C'\\ .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'G'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'C +38 h .t\CS.seek 0ml C'U'4 +506 h '4 C'$& h &W C'm% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C'D'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'a% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'h'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'l'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C''( +416 h ( M&N& h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &y'η +90 h 'η .tHand.open M&r1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Completed [coreNEG] begun 06:00:15 #\Preparing to sleep until Sat Sep 01 06:00:00 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST01-Sep-18 #\Continuing at 06:00CEST01-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:21:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST01-Sep-18" +235164 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118aug3107h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*f  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*/% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*.  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *1' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18aug3109h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*<% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*i  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +21 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*R  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*(  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*\ '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*+'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18aug3109h.pcr F*G% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*O  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +87 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *9'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*K% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*h'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *X'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18aug3112h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*4  +11 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*! I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*1% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*# n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*V  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*k'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*0  +108 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*c'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*d  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*%% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18aug3112h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*R'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18aug3114h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*P'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*v  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*-' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*p  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18aug3114h.pcr F*/% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*Y% h %Z ."MFBPCR"\AS.seek 0.81ml F*.'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*A% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*V  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*c % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*<'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug3116h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*F  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*\\% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*"' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*O  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +21 h  ."MFBPCR"delay 7 seconds +700 F*m% h %< ."MFBPCR"\AS.seek 0.594ml F*U'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*K n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*u  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*X % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218aug3116h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*N  +22 h ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*W'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +65 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*{% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*T'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18aug3119h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*0% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*H n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*c  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*% I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*j % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +21 h n ."MFBPCR"delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*(  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18aug3119h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*\  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Z  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +424 h ' ."MFBPCR"\ATV.dial :ASV F*= n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F*g% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*\\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *$' +424 h ' ."MFBPCR"\ATV.dial :air3 F*"% h % ."MFBPCR"\AS.seek 0.53ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*e% h % ."MFBPCR"\AS.seek 0.71ml F*K' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*U% h % H ."MFBPCR"\AS.seek 0.735ml F*u' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*D% h % Ű ."MFBPCR"\AS.seek 0.72ml F*n' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*h n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*w% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*?% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F* % h % ."MFBPCR"\AS.seek 0.579ml F*P' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %  ."MFBPCR"\AS.seek 0.604ml F*v'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*W' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*q % +107 h % F*-% h %V ."MFBPCR"\AS.seek 0.01ml F*p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*@% h % ."MFBPCR"\AS.seek 0.58ml F*^' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*B'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*2% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*;' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*{  +21 h  ."MFBPCR"\AS.seek 0ml F*f'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*4  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*~ n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *T'R +202 h 'R ."MFBPCR"\SPE.coast F*b  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*!  +43 h  F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *.' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*# I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*V  +43 h  F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*0  +65 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *S'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*H n +65 h n F*k% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Q' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*_  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*j% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*d n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *>' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*< I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*i  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*8 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *]'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*s n +150 h n ."MFBPCR"\AS.seek 0ml F*J'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*R  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*M % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*F'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F* 'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*= I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *g'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\\'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *G' +9987 h ' ."MFBPCR"\ATV.dial AIV F*" I +43 h I ."MFBPCR"\AS.seek 0.02ml F*$' +9880 h ' ."MFBPCR"\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.seek 0ml F*e'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*C% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*U  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*w'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*X'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*-% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*T % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 ."shutdown"\Elbow.coast M&5 +2 &6 C'5 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1535774400.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )! h  C' '1 h  P% %Y h  F* *# h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*.  h  .t\SPE.configure SPEconfig F*\ 2 *A% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)V% h % .t\SS.configure SSconfig C'6& S)#.0X/ .t\Elbow.jog 1500 M&i* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C''4 S)#.0X 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:22:16CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +150 h 1 M&=& h &_ M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &`(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &\\'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +264 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'$& h &W .tHand.open M&e1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'a& h &W C'9% h %4 .t\CSV.dial CSR C'u  +150 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2009 h '4 #t\Purging external Intake C'?& h &W C'% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N 'X  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'r'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'P  +75 h #t\Flushing upper manifold C'& h &W C'y% h %4 .t\CSV.dial :flush C'v  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +37 h @ C'L% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'q .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'-'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'@'4 +507 h '4 C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'p'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +38 h @ C'^% h %0 .t\CS.seek 5ml C'2'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'{ +75 h .t\CS.seek 0ml C';'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +7 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'b( +416 h ( M&~& h &ӷ M&T% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&l& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:22:20CEST30-Aug-18\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:22:16CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*I% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&!% h %\  A(1% h %X A(Y. h ., A(#& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (.& h &  M&A& h &- M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &6( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*0 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *5' +160 h ( M&t& h & A(H& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A(S& h &  .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(Q& h &  M&c& h & .t\Elbow.seek 2 M&_( +7 h ( M&%& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&|'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &d/ +150 h / ."downElevator"\SE.seek :down M&j& A('X h & M&% h %; .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.seek CC M&s(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &(' +90 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&\ 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)M+ +7 h + S)J% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % .t\CS.seek 6ml C'b' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'\ +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b '' +710 h ' .t\CSV.dial CSR C'J  +150 h  C'% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '(' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '8% h %  .t\CS.seek 4.5ml C'R'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'M  +38 h  .t\CS.seek 1.275ml C' ' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'=   '<9 h 9d +100 C'g9 h 9d +100 C'+9 h 91d +100 C'9 h 9J d +100 C'9 h 9bd +100 C'9 h 9zd +100 C'59 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'"9 h 9$d +100 C'G9 h 9(d +100 C'`9 h 9 ,d +100 C'9 h 9$0d +100 C'Z9 h 9<4d +100 C'$9 h 9S8d +100 C'e9 h 9kd +100 C'y9 h 9Td +100 C'9 h 9kd +100 C'v9 h 9d +100 C'r9 h 9d +100 C'L9 h 9d +100 C' 9 h 9d +100 C'q9 h 9d +100 C'9 h 9d +100 C'-9 h 9 d +100 C' 9 h 9 d +100 C'9 h 96d +100 C'9 h 9Ld +100 C'}9 h 9bd +100 C'9 h 9xd +100 C'W9 h 9d +100 C'@9 h 9d +100 C'o9 h 9d +100 C'^9 h 9d +100 C'9 h 9d +100 C'p9 h 9d +100 C'B9 h 9d +100 C'{9 h 9(d +100 C'f9 h 9>d +100 C'29 h 9Td +100 C'/9 h 9id +100 C'9 h 9d +100 C'49 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'K9 h 9d +100 C'C9 h 9d +100 C'T9 h 9  d +100 C'l9 h 9 d +100 C'O9 h 9 *d +100 C'9 h 9 ?d +100 C')9 h 9 Td +100 C'9 h 9 j d +100 C'~9 h 9 $d +100 C'79 h 9 (d +100 C'9 h 9 ,d +100 C'I9 h 9 0d +100 C'!9 h 9 4d +100 C'19 h 9 8d +100 C'Y9 h 9 9 h 9 Xd +100 C'9 h 9 kd +100 C'\ 9 h 9 ~d +100 C'b9 h 9 d +100 C'J9 h 9 d +100 C'9 h 9 d .t\CH.hold 5:00,85C C'   P h  +30000  C'89 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'('4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'M @ +113 h @ .t\CSV.dial :air C'R @ +38 h @ .t\CS.seek 5ml C'<'p +1372 h 'p .t\CSV.dial CTV C'g  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'+ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'5 +113 h .t\CBV.dial CSV C'  +18 h C'% h %4 .t\CS.seek 5ml C'"'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'G  +37 h C'`% h %p .t\CS.seek 7ml C''i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'Z @ +37 h @ .t\CS.seek 10ml C'$' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'e ` +132 h ` #tLysing Complete C'U& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&a% h %\  A(E% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (m( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&n& h &ӷ M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &u( +143 h ( M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &?' +89 h ' A(& h & .t\SE.seek 4.71 Pucks below Top Plate A(3' +214 h ' M&D& h & A(X& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &N'Y +26 h 'Y .t\Elbow.hold M&P, +6 h , .t\Elbow.reconfigure TightElbow A( - h -+ M&$&.,(\ #b .t\SE.seek 5.04 Pucks below Top Plate A(;' +27 h ' M&y% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&v'υ +20 h 'υ M&r& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +23 h ' .t\SE.seek 6.04 Pucks below Top Plate A(q' +36 h ' M&& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'@( +416 h ( A(o& h & A(^& h & M&& h &ӷ M&p% h % .t\Forearm.seek :retract M&B'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &{( +143 h ( M&f& h & A(2& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &/'Y +92 h 'Y .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &41 +150 h 1 M&% h %Y A( & h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&K'υ +20 h 'υ M&C& h & A(T& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &l' +22 h ' A(O% h % ."downElevator"\SE.seek :down A('X +100 ()% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +90 h '\  +12 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'7 @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C'I ` +18 h ` .t\AIV.dial :bypass C'! { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&Y1 +2 h 1 M&#& h & M&.% h %\  .t\Elbow.seek FlushPuck::Garage M&[(- +166 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&0'η +91 h 'η .tHand.close M&/ +150 h / M&H& h &- M&z% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +265 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C''(W +416 h (W C'Q& h &W .tHand.open M&j1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%6' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%k  +37 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%i' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%|  C'  h +75 .t\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%>  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%\  +38 h  .t\PS.seek 2.5ml P%b' +2609 h ' .t\PSV.dial :mixloop P%J +38 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%8' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%M' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +7 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'<( +416 h ( M&5& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &"'η +91 h 'η .tHand.open M&Z1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'e& h & M&U% h %\  A(a% h %X A(E. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (h( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&n& h &- M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &w( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &s' +90 h ' A(&& h &  .t\SE.seek 13.71 Pucks below Top Plate A(D'n +125 h 'n A(X& h &  M& & h & .t\Elbow.seek 2 M&y( +7 h ( M&& h & A(r& h &  .t\Forearm.seek 2 + 300 counts M&L'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&-& h & A(?'X M&u% h %; .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.seek CC M&N(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C';(W +416 h (W .tHand.open M& 1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C',  +18 h  .t\CS.seek 5ml C'o'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'^' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%p  +38 h  .t\PS.seek 2.5ml P%f' +2609 h ' .t\PSV.dial :mixloop P%2 +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%4  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'C  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'T @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'O  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'B ` +19 h ` .t\CSV.dial :air C'{ @ +38 h @ .t\CS.seek 10ml C')' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'7'4 +2648 h '4 .t\CBV.dial :waste1 C'I @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'!  +151 h C'1& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&Y% h %\  A(#% h %X A(.. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (/( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (K& h & M&q& h &ӷ M&l% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &V( +143 h ( M&x& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A($& h & .t\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' M&& h & A(H& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &z'Y +25 h 'Y .t\Elbow.hold M&~, +7 h , .t\Elbow.reconfigure TightElbow A(0- M&$&.,(\ #b h -+ .t\SE.seek 6.04 Pucks below Top Plate A(:' +27 h ' M&c% h %Y A([& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&S'υ +21 h 'υ M&@& h & A(Q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &j' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(A' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +89 h '\  .t\Elbow.seek CC M&k(ӷ +142 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'( +416 h ( A(_& h & A(>& h & M&i& h &ӷ M&\ % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &J( +143 h ( M&b& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +92 h 'Y .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &81 +150 h 1 M&R% h %Y A(g& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &+'υ +21 h 'υ M&& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A('% h % ."downElevator"\SE.seek :down A(5'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +90 h '\  + 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%`  +2 A(6 +111 h  P%(% h % .t\PS.seek 0.499ml P%Z' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%= +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%e  +38 h .t\CBV.dial AIV C'U  +37 h .t\PRV.dial between CBV and air P%a  +19 h  .t\PRV.dial :air P%E  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %n' +250 h ' .t\PRV.dial :waste2 P%9 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %"' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&X(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&-% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +265 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'L(W +415 h (W C'D& h &W .tHand.open M&y1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  C'<& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%P  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%,% h % .t\PS.seek 1.499ml P%v' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%h' +1095 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%o' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%^ +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%p  +38 h  .tdelay 2 seconds +200 P%M% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %2'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%4' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%f  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%C  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%T  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %O' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%B +76 h .t\PTV.dial :PRV,avoiding: :puck P%{ @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%)' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%I +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%!' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%1  +75 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%# +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%.  +76 h P%K% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %q' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'l & +50 h & .t\AWV.select :waste C' +6 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %m'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'$ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %N' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%H' +2609 h ' .t\PSV.dial :PML P%W +38 h .t\PS.seek 0ml P%0' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'c& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'[& F*@ @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*Q  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*V I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *z'6 +66 .t\PSV.dial :waste1 P%6  +37 h  .t\PS.seek 0ml P%*' +1597 h ' .t\PSV.dial PSR P%S +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%:' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%j +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%~' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*|  +22 h ' .t\PSV.dial PSR P%% +21 h  F*;% h %6 ."MFBPCR"\AS.seek 0.329ml F*_'ͯ +92 h P%>% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%i' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*\ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%]' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*k  +22 h  F*8% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *R' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%g +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*t I +43 h I F*'% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%+' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +37 h  .t\PS.seek 0ml P%`' +740 h ' ."MFBPCR"delay 5 seconds +500 F*(9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*d n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*5% h % ."MFBPCR"\AS.seek 0.041ml F*/'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%e' +2610 h ' .t\PSV.dial PSR P%U +113 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%E' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%Z' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%9 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%n' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %&'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb % ' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b * 'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%- +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %G' +5107 h ' .t\PSV.dial :air P%r  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %s' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*  +42 h  F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *u'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*D% h %Q ."MFBPCR"\AS.seek 0.01ml F*y' +171 h ' .t\PSV.dial :mixloop P%? +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %<' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*}'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*P  +86 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *,' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*"  +43 h F*v% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'h  +38 h  C'^% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'M' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'2'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*f  +22 h ."MFBPCR"delay 3 seconds +300 F*4% h %_ ."MFBPCR"\AS.seek 0.434ml F*C'ݏ +109 h '4 .t\CSV.dial CSR C'T  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'B'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*{  +22 h F*O% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*I% h %& ."MFBPCR"\AS.seek 0.092ml F*)'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'1' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'!  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'#'4 +2647 h '4 C'.& h &W #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +6 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'm( +416 h ( M&& h &ӷ M&N% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +265 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Q'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'V& h & M&% h %\  A(6% h %X A(\\. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (S& h &  M&*& h &- M&j% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &:( +166 h ( M&|& h & A(;& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(>& h &  ."WCR"\SE.seek 12.71 Pucks below Top Plate A(z' +136 h ' A(~& h &  M&\ & h & ."WCR"\Elbow.seek 2 M&_( +7 h ( M&& h & A(k& h &  ."WCR"\Forearm.seek 2 + 300 counts M&8'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &i/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down M&g& A(b'X h & M&t% h %; ."WCR"\Forearm.seek :retract M&''\ +91 h '\  ."WCR"\Elbow.seek CC M&](ӷ +45 h 'X ."downElevator"\SE.coast A(5 +98 h (ӷ M&R& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +34 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +13 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&A1 +131 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +19 h 1 M&(% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +3 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *5' +88 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'`& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %  ."MFBPCR"\AS.seek 0.604ml F*H'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*\ % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*k n +85 h n ."MFBPCR"\ASV.dial :wasteR F*0  +43 h  ."MFBPCR"\AS.seek 0ml F*i'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*: % +108 h % F*%% h %V ."MFBPCR"\AS.seek 0.59ml F*R' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +107 h n ."MFBPCR"\ASV.dial :wasteR F*z% h %  ."MFBPCR"\AS.seek 0.09ml F*8' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*_ I +86 h I F*b% h % ."MFBPCR"\AS.seek 0.12ml F*'' +625 h ' ."MFBPCR"\ATV.dial :air3 F*) % +22 h % F*m% h % ."MFBPCR"\AS.seek 0.13ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*+% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*a n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *E'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*= @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*/ I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*-'\  +1032 h '\  F*&% h %\  ."MFBPCR"\AS.seek 0.5ml F*G'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *X' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*r  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep0109h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*D  +65 h  ."MFBPCR"\ARV.dial :carrier F*?  +10 h  F*n% h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *P'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *u'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*,  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*h' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*p  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*4  +107 h  ."MFBPCR"delay 7 seconds +700 F*T% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*I  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*!% h % \\ ."MFBPCR"\AS.seek 0.326ml F*1'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*K % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254601 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'l  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'. @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '$' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'w @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'L  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'3 ` +19 h ` ."WCR"\CS.seek 7.288ml C'q' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'N  'c  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Y'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '[' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'\\' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'V @ +75 h @ +50 S)6% h % ."WCR"\CBV.dial between CSV and waste2b C'j  +94 h  C'Q% h % ."WCR"\CSV.dial between air and CTV C'* ` +57 h ` ."WCR"\CS.seek 7.288ml C';' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'>  '|  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'H' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'0  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'i' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C': @ +75 h @ +50 S)%% h % ."WCR"\CBV.dial between CSV and waste2b C't  +95 h  C'J% h % ."WCR"\CSV.dial between air and CTV C'z ` +56 h ` ."WCR"\CS.seek 7.288ml C'R' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '_  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'b'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'8  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ')' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'm  +151 h C''& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&(% h %\  A(+% h %X A(A. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (`( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(a& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &U' +90 h ' A(& h & ."WCR"\SE.seek 6.71 Pucks below Top Plate A(' +195 h ' M&e& h & A(E& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &/'Y +26 h 'Y ."WCR"\Elbow.hold M&Z, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(' +27 h ' M&9% h %Y A(5& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&& h & A( & h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A(&'w +36 h 'w M&-& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +89 h '\  ."WCR"\Elbow.seek CC M& (ӷ +143 h (ӷ M&X& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ ."WCR"\Forearm.seek CC M&D' +91 h ' ."WCR"Hand.close M&?/ +150 h / ."WCR"\CC.seek :open C'n( +416 h ( A(& h & A(s& h & M&y& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&P'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&"& h & A(v& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b & 'Y +92 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &^1 +150 h 1 M&u% h %Y A(p& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&o'υ +20 h 'υ M&h& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &M' +22 h ' A(7% h %w ."downElevator"\SE.seek :down A(4'X +100 (T% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'2& h & .tHand.open M& 1 +2 h 1 M&O& h & M&{% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&I'η +91 h 'η .tHand.close M&!/ +150 h / M&& h &- M&B% h %η .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +264 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'L& h &W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'c& h &W C'N% h % .t\CSV.dial CSR C'q  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'Y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '['4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'\\& h &W C'V% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'j'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +38 h @ C'*% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CTV C'>  .t\CTV.dial CSV C'|  +38 h  +113  .t\CBV.dial :waste1 C'; @ +75 h @ .t\CS.seek 0ml C'\ '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'~& h &W C'k% h %4 .t\CSV.dial :flush C'0  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C'i% h %p .t\CS.seek 7ml C':'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'%  +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'J'4 +766 h '4 #t\Flushing upper manifold C'z& h &W C'g% h %4 .t\CSV.dial :flush C'_  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'8% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'm ''  +38 h +75 .t\CTV.dial :air C'(  +151 h .t\CS.seek 0ml C'+'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'E'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'd'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'9 +75 h .t\CS.seek 0ml C'5'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C' % h %4 .t\CSV.dial CSR C'-  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'/  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'X' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'r  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'Z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C's'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'y  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '"'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'^'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'u  +38 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'h'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P '7  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'T'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C'O& h &W C'{% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'o'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +38 h @ C'C% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M +113 h .t\CTV.dial :air .\CS.seek 0ml C'4'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'`'0 +506 h '0 .t\CSV.dial :waste C', +38 h .t\CS.seek 0ml C'!'4 +507 h '4 C'& h &W C'B% h %4 .t\CSV.dial :flush C'f  +37 h  .t\CS.seek 3ml C'I'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +37 h @ C'#% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +506 h '0 .t\CSV.dial :waste C'L +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'3& h &W C'c% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.seek 5ml C'q'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'w% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  'Y  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\\& h &W C'V% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +38 h @ C'*% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'>  +38 h  .t\CBV.dial :waste2b C'|  +75 h  .t\CS.seek 0ml C';'4 +766 h '4 #tDisinfecting external Intake with kill C'S& h &W C'~% h %4 .t\CSV.dial CSR C'k  +38 h  C'0% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'\ 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'H' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'i  ' +38 h +75 .t\CTV.dial :intake C'%  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C't' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C':  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'z'4 +607 h '4 .t\CSV.dial CSR C'g  +113 h  C'_% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'J'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'R' +1372 h ' .t\CSV.dial CBV C'8 +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&'& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'A( +415 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&E1 +150 h 1 M&d% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &-(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.seek :garage M&U'η +91 h 'η .tHand.close M&/ +150 h / M&/& h &- M&% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C'G& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'y& h &W C' % h %4 .t\CSV.dial CSR C's  +113 h  C'"% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'v'[ +463 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Purging external Intake C'^& h &W C'h% h %4 .t\CSV.dial :flush C'7  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'T'4 +910 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'2  +75 h #t\Flushing upper manifold C'& h &W C'O% h %4 .t\CSV.dial :flush C'{  +75 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'&% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV C'o .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'M'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'4 +38 h .t\CS.seek 0ml C','4 +507 h '4 C'`& h &W C'% h %4 .t\CSV.dial :flush C'B  +37 h  .t\CS.seek 3ml C'f'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'.% h %0 .t\CS.seek 5ml C'#'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'I'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'K +76 h .t\CS.seek 0ml C'L'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&n& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +7 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'w( +416 h ( M&q& h &ӷ M&W% h % .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &V'η +91 h 'η .tHand.open M&61 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Completed [mbaPhase] begun 06:00:15 #\Preparing to sleep until Sun Sep 02 06:00:00 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST02-Sep-18 #\Continuing at 06:00CEST02-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:23:46CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST02-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0109h25ml.pcr F**% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*>  +21 h ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +65 h  F*g% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*b  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*E% h %m ."MFBPCR"\AS.seek 0.82ml F*d'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep0111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*y  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*"  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*v  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*h'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*7  +107 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*T  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep0111h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*` % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*.'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*I'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*N  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *X'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*@'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*j% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*l  +21 h ."MFBPCR"delay 2 seconds +200 F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*] n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*m' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*E  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*5'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep0113h25ml.pcr F*-% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*r  +21 h ."MFBPCR"\ATV.dial :air3 F*G % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*v'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*2  +32 h  ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*o% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +21 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*4  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep0116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +10 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*3  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*Y n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F** I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*1% h % ."MFBPCR"\AS.seek 0.53ml F*@' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*| % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.536ml F*>'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +107 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*S n +21 h n ."MFBPCR"delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*i  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*:  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep0116h25ml.pcr F*g% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*R  +22 h ."MFBPCR"\ATV.dial :air3 F*8 % +87 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*+'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*e  +65 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*5 % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep0118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*7  +42 h  ."MFBPCR"\ARV.dial :carrier F*v  +11 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*{ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*=  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*C' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*4  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*#  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*X % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep0118h25ml.pcr F*N% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*q  +22 h ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*1% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +64 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*%% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep0120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ARV.dial :carrier F*+  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*U I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*/% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*9 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*r  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.65ml F*D' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*7'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep0120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*u @@T ."MFBPCR"\ASV.dial :SPEdelivery F*h  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *2' +423 h ' ."MFBPCR"\ATV.dial :ASV F*T n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*{'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*o n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *='V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*M'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*& % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *`' +424 h ' ."MFBPCR"\ATV.dial :air3 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*4' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*!' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h % H ."MFBPCR"\AS.seek 0.735ml F*#' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*f% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*I n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*c% h % zG ."MFBPCR"\AS.seek 0.365ml F*K' +7161 h ' ."MFBPCR"\ATV.dial :waste F*n n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*W% h % ."MFBPCR"\AS.seek 0.579ml F*?' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %  ."MFBPCR"\AS.seek 0.604ml F*\\'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*V% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +86 h n ."MFBPCR"\ASV.dial :wasteR F*1  +22 h  ."MFBPCR"\AS.seek 0ml F*)'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *j'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*@ % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*>% h % ."MFBPCR"\AS.seek 0.58ml F*S' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F*i% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*%  +21 h  ."MFBPCR"\AS.seek 0ml F*:'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *g'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*_  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *R' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*8 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *H'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*J n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *''R +202 h 'R ."MFBPCR"\SPE.coast F*l  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *b' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*E  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *+': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*d  +64 h  F*e% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*a% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b */': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*9% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *-'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*r% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *y'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*s'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*"  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*D% h % ."MFBPCR"\AS.seek 0.96ml F*v'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %0 ."MFBPCR"\AS.seek 0.985ml F*7' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*P'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*T I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*=  +43 h  F*&% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *M' +9988 h ' ."MFBPCR"\ATV.dial AIV F*C I +43 h I ."MFBPCR"\AS.seek 0.02ml F*`' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*4% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F*! % +43 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*I% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*X'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*W  +64 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*)% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*~'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*k% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' @CEST1535839201.01 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *H'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*' % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2133925 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '$ h  P% %( h  F* *] h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *b  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)E% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'm& S)#.0X 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:24:41CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&71 +150 h 1 M&T& h &_ M&P% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &o'η +91 h 'η .tHand.close M&{/ +150 h / M&=& h &- M&&% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'4(W +416 h (W C'`& h &W .tHand.open M& 1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'.& h &W C'B% h %4 .t\CSV.dial CSR C'#  +150 h  C'I% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'f'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'c +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'N'4 +2009 h '4 #t\Purging external Intake C'& h &W C'K% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'q 'X  h +75 .t\CTV.dial :intake C'3  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'6'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'?  +75 h #t\Flushing upper manifold C'Y& h &W C'V% h %4 .t\CSV.dial :flush C'\\  +75 h  .t\CS.seek 5ml C'1'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C')% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'@ +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'|'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'>'4 +507 h '4 C'j& h &W C';% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'S% h %0 .t\CS.seek 5ml C'i'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'% +75 h .t\CS.seek 0ml C'0'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&:& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &z(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M&8' +91 h ' .tHand.close M&R/ +150 h / .t\CC.seek :open C'l( +416 h ( M&J& h &ӷ M&'% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &w'η +91 h 'η .tHand.open M&L1 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:24:46CEST30-Aug-18\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:24:41CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*Q% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'u& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A($% h %X A((. h ., A(]& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (b& h &  M&& h &- M&E% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &m( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*d % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *e' +160 h ( M&+& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &a' +89 h ' A(& h &  .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ A(& h &  M&5& h & .t\Elbow.seek 2 M&( +7 h ( M&U& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&9'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&r& A('X h & M&-% h %; .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +54 h 'X ."downElevator"\SE.coast A(5 +88 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'p(W +416 h (W .tHand.open M&h1 +150 h 1 M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)s% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 S)\ % h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)S +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'V9 h 9Td +100 C'q9 h 9kd +100 C'\\9 h 9d +100 C'69 h 9d +100 C')9 h 9d +100 C'19 h 9d +100 C'@9 h 9d +100 C'*9 h 9d +100 C'9 h 9 d +100 C'N9 h 9 d +100 C'Y9 h 96d +100 C'|9 h 9Ld +100 C'j9 h 9bd +100 C';9 h 9xd +100 C'9 h 9d +100 C'>9 h 9d +100 C'\ 9 h 9d +100 C'S9 h 9d +100 C'k9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'%9 h 9(d +100 C':9 h 9>d +100 C'i9 h 9Td +100 C'g9 h 9id +100 C'[9 h 9d +100 C'_9 h 9d +100 C't9 h 9d +100 C'R9 h 9d +100 C'!9 h 9d +100 C'49 h 9d +100 C''9 h 9  d +100 C'F9 h 9 d +100 C'9 h 9 *d +100 C'89 h 9 ?d +100 C'9 h 9 Td +100 C'L9 h 9 j d +100 C'J9 h 9 $d +100 C'z9 h 9 (d +100 C'H9 h 9 ,d +100 C'Q9 h 9 0d +100 C'9 h 9 4d +100 C'$9 h 9 8d +100 C'(9 h 9 ( +416 h ( A(\ & h & A(S& h & M&k& h &ӷ M&~% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &%( +143 h ( M&:& h & A(i& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &g'Y +92 h 'Y .t\Elbow.hold M&[, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &_1 +150 h 1 M&t% h %Y A(R& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&!'υ +20 h 'υ M&4& h & A('& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &F' +22 h ' A(% h %f ."downElevator"\SE.seek :down A(8'X +100 (% h % M&L% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +73 h 'X ."downElevator"\SE.coast A(5 +17 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'z @ +19 h @ .t\CS.seek 10ml C'H' +6 h ' .t\CSV.dial between air and CTV C'Q ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'$& h & .tHand.open M&(1 +2 h 1 M&]& h & M&b% h %\  .t\Elbow.seek FlushPuck::Garage M&<(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&d'η +91 h 'η .tHand.close M&u/ +150 h / M& & h &- M&w% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &U' +91 h ' .t\CC.seek :closed C'}(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%a  +38 h  .t\PS.seek 0ml P%-' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%9  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%G  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%h  +37 h  .t\PS.seek 2.5ml P%l' +2610 h ' .t\PSV.dial :mixloop P%s +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%/  +38 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :mixloop P%T +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.seek CC M&7' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'( +416 h ( M&e& h &ӷ M&D% h % .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +265 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&1 +150 h 1 M&P% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&,% h %\  A(.% h %X A(O. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (I( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &  M&& h &- M&B% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &c( +166 h ( M&n& h & A(K& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(f& h &  .t\SE.seek 10.71 Pucks below Top Plate A(X' +155 h ' A(& h &  M&W& h & .t\Elbow.seek 2 M&V( +7 h ( M&q& h & A(6& h &  .t\Forearm.seek 2 + 300 counts M&)'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&#% h %; .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.seek CC M&3(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +78 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'0(W +416 h (W .tHand.open M&11 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'N  +151 h  .t\CSV.dial CTV C'p  +19 h  .t\CS.seek 5ml C'\ 'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'S' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'k'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%:' +2609 h ' .t\PSV.dial :mixloop P%i +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%;  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Y +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%_  +151 h  .t\PS.seek :empty P%t' +2610 h ' .t\CBV.dial :waste1 C'R @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'4  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'% @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'L  +38 h  .t\CS.seek 0ml C'z'4 +2647 h '4 .t\CBV.dial :waste1 C'Q @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'$& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&(% h %\  A(]% h %X A(b. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (g( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (!& h & M&@& h &ӷ M&F% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &E( +143 h ( M&x& h & A(8& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(`& h & .t\SE.seek 8.71 Pucks below Top Plate A([' +176 h ' M&u& h & A( & h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &w'Y +25 h 'Y .t\Elbow.hold M&J, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(d- h -+ .t\SE.seek 9.04 Pucks below Top Plate A('f +26 h 'f M&5% h %Y A(<& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&>& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 10.04 Pucks below Top Plate A('U +36 h 'U M&& h & M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  .t\Elbow.seek CC M&a(ӷ +143 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(G& h & M&& h &ӷ M&h% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &s( +142 h ( M&l& h & A(/& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&T, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &"1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&2'υ +20 h 'υ M&=& h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +23 h ' A(}% h %U ."downElevator"\SE.seek :down A(e'X +100 (D% h % M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +61 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P% % h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%P +37 h .t\PRV.dial :air P%{  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C',  +37 h .t\PRV.dial between CBV and air P%.  +19 h  .t\PRV.dial :air P%O  +19 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%B @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&K1 +2 h 1 M&f& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&W& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&6'η +91 h 'η .tHand.close M&/ +150 h / M&*& h &- M&% h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &j' +90 h ' .t\CC.seek :closed C')(W +416 h (W C'X& h &W .tHand.open M&V1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%1 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%?  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%N  +75 h  .tdelay 2 seconds +200 P%p% h % .t\PS.seek 1.499ml P%\\' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%I' +1094 h ' .t\PSV.dial :air P%H  +38 h  .t\PS.seek 0ml P%\ ' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%S +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%~  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %i'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%;  +75 h .tdelay 2 seconds +200 P%Y% h %r .t\PS.seek 0.475ml P%_' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%:  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%R'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%4  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%'  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck P%% @ +75 h @ .t\PS.seek 0ml P%k' +2610 h ' .t\PSV.dial :air P%L  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%$  +75 h  .t\PS.seek 2.5ml P%(' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%] +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%b  +75 h P%!% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %@' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'F & +50 h & .t\AWV.select :waste C' +6 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %8'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'` { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %3' +1313 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P% ' +2609 h ' .t\PSV.dial :PML P%d +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '5& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C F*> @@T C'<& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%r% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*E I +129 h I F*[% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *w'6 +66 .t\PSV.dial :waste1 P%m  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%U +21 h  F*0% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%G% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*h % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%/  +38 h  .t\PS.seek 0ml P%-' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*a  +22 h  F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%=' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*}% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%s +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial PSR P%7 +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P% ' +740 h ' ."MFBPCR"delay 5 seconds +500 F* 9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*y n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*e% h % ."MFBPCR"\AS.seek 0.041ml F*g'% +336 h ' .t\PSV.dial PSR P%t +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%P' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%{ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%, +113 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%B +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%K +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %f'k +2678 h 'k .t\PSV.dial :waste1 P%&  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %W' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*q I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *T'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%*' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %M' +5107 h ' .t\PSV.dial :air P%6  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*|  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *#'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*X% h %Q ."MFBPCR"\AS.seek 0.01ml F*V' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.seek :empty F*j'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*?  +86 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *p' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*\\% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *H'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'I  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\  ';  +38 h  +113  .t\CBV.dial :waste1 C'Y @ +37 h @ .t\CS.seek 0ml C'i'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*:  +21 h ."MFBPCR"delay 3 seconds +300 F*_% h %_ ."MFBPCR"\AS.seek 0.434ml F*4'ݏ +110 h '4 .t\CSV.dial CSR C''  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*%  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*L'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*k n +108 h n F*Q% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'$' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C']'4 +2647 h '4 C'b& h &W #tGaraging FlushPuck in Collection Clamp M&!& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +6 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'8( +416 h ( M&u& h &ӷ M&3% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'E& h & M&[% h %\  A(m% h %X A(o. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&^& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(0& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +89 h ' A(G& h &  ."WCR"\SE.seek 9.71 Pucks below Top Plate A(w' +166 h ' A(J& h &  M&h& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&/& h & A(a& h &  ."WCR"\Forearm.seek 2 + 300 counts M&"'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +88 h '&0 ."MFBPCR"delay 3 seconds +62 h / ."downElevator"\SE.seek :down M&& A(l'X h & M&+% h %; ."WCR"\Forearm.seek :retract M&}'\ +91 h '\  ."WCR"\Elbow.seek CC M&-(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +67 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +5 ."MFBPCR"\ATV.dial :waste2 F*7 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +43 h ' ."WCR"\CC.seek :closed C'D(W +416 h (W ."WCR"Hand.open M&1 +101 h 'R ."MFBPCR"\ATV.dial :air2 F*2  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b * ' +27 h 1 M&y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)t% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'{  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:26:12CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST03-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0209h25ml.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*9  +21 h ."MFBPCR"\ATV.dial :air3 F*G % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*l  +32 h  ."MFBPCR"\ATV.dial :air3 F*{ % +86 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*=% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +21 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*.  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep0211h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*,  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F**  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*C  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*T  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*#' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\\  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*I'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*?  +108 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*i'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*'  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*R  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*4 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep0211h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*L  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep0213h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*= I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*- n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*8' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*O  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*.'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +108 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*q'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +22 h n ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*B % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep0213h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*6  +22 h ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*M% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*S  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*#  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*I' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*i  +31 h  ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*N'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*_  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *3'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep0216h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*^ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*>' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*G % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*9'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +108 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep0216h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +21 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*8% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +21 h F*D% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*2  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep0218h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*I'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*i  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +22 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*% n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*P  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*L  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*N'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +22 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*]  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*H  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep0218h25ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*m% h %Z ."MFBPCR"\AS.seek 0.81ml F*u'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*$% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*w% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *F'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep0220h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +11 h  F*K% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*W n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +129 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*, I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*B % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*6  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*2  +22 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +22 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*X' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*T  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*S% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep0220h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*# @@T ."MFBPCR"\ASV.dial :SPEdelivery F*I  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Y  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *i' +423 h ' ."MFBPCR"\ATV.dial :ASV F*' n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*R  +43 h  F*;% h % ."MFBPCR"\AS.seek 0.62ml F*%'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*\ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *P'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :air3 F*4% h % ."MFBPCR"\AS.seek 0.53ml F*_' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +21 h F*N% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h % H ."MFBPCR"\AS.seek 0.735ml F*]' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*:% h % Ű ."MFBPCR"\AS.seek 0.72ml F*H' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*Q n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*5% h % zG ."MFBPCR"\AS.seek 0.365ml F*!' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*3 % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*d% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %  ."MFBPCR"\AS.seek 0.604ml F*o'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*E% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*$  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*> % +108 h % F*r% h %V ."MFBPCR"\AS.seek 0.01ml F*J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*9% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +21 h F*/% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*+ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*w n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b * '^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*s n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +202 h 'R ."MFBPCR"\SPE.coast F*F  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*{ n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*=  +43 h  F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *l' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*- I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*O  +43 h  F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *y': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*K  +64 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *e'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*W n +65 h n F*.% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *|' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*q  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *g': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*B% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*t n +65 h n F*6% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *D' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F**  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*M n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *V'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*1 n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*n % +43 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*X% h % ."MFBPCR"\AS.seek 0.96ml F*\\'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %0 ."MFBPCR"\AS.seek 0.985ml F*?' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*z'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*' I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *;'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\ 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*P  +43 h  F*f% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*4 I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F*k n +129 h n ."MFBPCR"\AS.seek 0ml F*N'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*_% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *:'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*Q% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*!'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +64 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Z'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*$  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +21 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' @CEST1535925600.87 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*} % +108 h % ."MFBPCR"\AS.seek 0ml F*{'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 F*5 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2133939 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )= h  C' '` h  P% % h  F* *- h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *l  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)O% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'8& S)#.0X 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:27:08CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #5) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&?1 +150 h 1 M&'& h &_ M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &R(- +166 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &\ 'η +91 h 'η .tHand.close M&%/ +150 h / M&P& h &- M&f% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +264 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C' & h &W .tHand.open M&N1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'b& h &W C'% h %4 .t\CSV.dial CSR C']  +150 h  C'Q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ':'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'H% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'5 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '3'4 +2009 h '4 #t\Purging external Intake C'& h &W C'!% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'd' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'@ '  h +75 .t\CTV.dial :intake C'Z  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'm'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'(& h &W C'E% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C'$'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +37 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV C'> .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'r'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'< +37 h .t\CS.seek 0ml C'9'4 +507 h '4 C'& h &W C'0% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C'J'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'U +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&w' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'F( +416 h ( M&s& h &ӷ M&}% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &{(- +264 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &c'η +91 h 'η .tHand.open M&v1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:27:12CEST30-Aug-18\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:27:08CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*j% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'#& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&=% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(`% h %X A(. h ., A(-& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (l& h &  M&& h &- M&O% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &8( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*K % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +160 h ( M&y& h & A(W& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &.' +89 h ' A(e& h &  .t\SE.seek 8.71 Pucks below Top Plate A(A' +176 h ' A(|& h &  M& & h & .t\Elbow.seek 2 M&q( +6 h ( M&,& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&B'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down M&& A(6'X h & M&% h %; .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.seek CC M&1(ӷ +85 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &T' +91 h ' .t\CC.seek :closed C'~(W +416 h (W .tHand.open M&I1 +150 h 1 M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)n+ +6 h + S) % h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +19 h  C'^% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'r  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '<'4 +2609 h '4 C'G% h %4 S)% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'0 @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'E9 h 9Td +100 C'Z9 h 9kd +100 C'o9 h 9d +100 C'm9 h 9d +100 C'9 h 9d +100 C'$9 h 9d +100 C'>9 h 9d +100 C'^9 h 9d +100 C'r9 h 9 d +100 C'39 h 9 d +100 C'(9 h 96d +100 C'G9 h 9Ld +100 C'9 h 9bd +100 C'09 h 9xd +100 C'u9 h 9d +100 C'99 h 9d +100 C'h9 h 9d +100 C'9 h 9d +100 C'a9 h 9d +100 C'J9 h 9d +100 C'9 h 9d +100 C'U9 h 9(d +100 C'9 h 9>d +100 C'/9 h 9Td +100 C'9 h 9id +100 C'<9 h 9d +100 C'9 h 9d +100 C'+9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'_9 h 9d +100 C'}9 h 9  d +100 C'[9 h 9 d +100 C'L9 h 9 *d +100 C'w9 h 9 ?d +100 C'9 h 9 Td +100 C'v9 h 9 j d +100 C's9 h 9 $d +100 C'79 h 9 (d +100 C' 9 h 9 ,d +100 C'j9 h 9 0d +100 C'=9 h 9 4d +100 C'`9 h 9 8d +100 C'9 h 9 '3 +36 h '3 M&^& h & M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  .t\Elbow.seek CC M&((ӷ +143 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&0' +91 h ' .tHand.close M&u/ +150 h / .t\CC.seek :open C'9( +416 h ( A(h& h & A(& h & M&a& h &ӷ M&J% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &U( +143 h ( M&& h & A(/& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 1 M&'Y +92 h 'Y .t\Elbow.hold M&<, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&+% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +20 h 'υ M&_& h & A(}& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &[' +22 h ' A(L% h %3 ."downElevator"\SE.seek :down A(w'X +100 (% h % M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +43 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'7 @ +19 h @ .t\CS.seek 10ml C' ' +6 h ' .t\CSV.dial between air and CTV C'j ` +19 h ` .t\AIV.dial :bypass C'= { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&1 +2 h 1 M&-& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&K'η +91 h 'η .tHand.close M&#/ +150 h / M&W& h &- M&c% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C')(W +416 h (W C'|& h &W .tHand.open M&t1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%8' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%.  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%S +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +2610 h ' ."connect"\PRV.dial CBV P%B  .t\CBV.dial PRV C'A  h +75 .t\PSV.dial :mixloop P%q +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%D  h .t\PS.seek 0ml P%6' +2610 h ' .t\PSV.dial :air P%I  +37 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%g  +38 h  .t\PS.seek 2.5ml P%C' +2610 h ' .t\PSV.dial :mixloop P%' +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&M& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +6 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&P/ +150 h / .t\CC.seek :open C'( +416 h ( M&f& h &ӷ M&X% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &4'η +91 h 'η .tHand.open M&Y1 +150 h 1 M&z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'N& h & M&p% h %\  A(b% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (Q( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (2& h &  M&H& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&& h & A(!& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(:& h &  .t\SE.seek 7.71 Pucks below Top Plate A(' +185 h ' A(d& h &  M&&& h & .t\Elbow.seek 2 M&E( +6 h ( M&Z& h & A(m& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +150 h / ."downElevator"\SE.seek :down M&r& h & A('X M&]% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&@(ӷ +93 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&$1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'3  +150 h  .t\CSV.dial CTV C'~  +19 h  .t\CS.seek 5ml C'h'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'a'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%J  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%/ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%0  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%( +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%+' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'} @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'L  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'U @ +37 h @ .t\CS.seek 10ml C'' +2648 h ' .t\CSV.dial CTV C'v  +37 h  .t\CS.seek 0ml C'7'4 +2647 h '4 .t\CBV.dial :waste1 C'j @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'=  +151 h C'`& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(-% h %X A(l. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&>& h &ӷ M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &O( +143 h ( M&x& h & A(w& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A( & h & .t\SE.seek 11.71 Pucks below Top Plate A(<'Ɛ +146 h 'Ɛ M&#& h & A(W& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &c'Y +25 h 'Y .t\Elbow.hold M&s, +7 h , .t\Elbow.reconfigure TightElbow A(K- h -+Ɛ M&$&.,(\ #b .t\SE.seek 12.04 Pucks below Top Plate A({'3 +26 h '3 M& % h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&e'υ +21 h 'υ M&9& h & A(|& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' .t\SE.seek 13.04 Pucks below Top Plate A('" +36 h '" M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&.(ӷ +143 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&B/ +150 h / .t\CC.seek :open C'A( +416 h ( A(q& h & A(D& h & M&*& h &ӷ M&I% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&F& h & A(g& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&', +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&M% h %Y A(;& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &i'υ +20 h 'υ M&P& h & A(y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +23 h ' A()% h %" ."downElevator"\SE.seek :down A(f'X +100 (X% h % M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +33 h 'X ."downElevator"\SE.coast A(5 +56 h '\  .t\SC.coast .\PSV.dial :air A(6 P%R  +113 h  P%T% h % .t\PS.seek 0.499ml P%Y' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%z +37 h .t\PRV.dial :air P%%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%N  +38 h .t\CBV.dial AIV C'p  +37 h .t\PRV.dial between CBV and air P%b  +19 h  .t\PRV.dial :air P%  +19 h  P%2% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %H' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %4' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&!1 +2 h 1 M&:& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&d(- +166 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&m'η +91 h 'η .tHand.close M&"/ +150 h / M&^& h &- M&r% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&E1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%$ @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%3  +75 h  .tdelay 2 seconds +200 P%~% h % .t\PS.seek 1.499ml P%o' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%Q' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%h' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%J  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %/'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%0  +75 h .tdelay 2 seconds +200 P%(% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%_  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%}  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %L' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck P%U @ +75 h @ .t\PS.seek 0ml P%a' +2610 h ' .t\PSV.dial :air P%v  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%j +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%=' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%`  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%- +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%l  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %>' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'[ & +50 h & .t\AWV.select :waste C' +6 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %w'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %@' +1313 h ' .t\PSV.dial :air P%#  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%K +38 h .t\PS.seek 0ml P% ' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'u& F*9 @@T h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass F*|  .t\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%k' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*O I +129 h I F*<% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *c'6 +66 .t\PSV.dial :waste1 P%8  +37 h  .t\PS.seek 0ml P%S' +1597 h ' .t\PSV.dial PSR P%e +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%{' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*B  +22 h ' .t\PSV.dial PSR P%, +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*q'ͯ +92 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%*' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*I % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *F'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%g  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*.  +22 h  F*C% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *M' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%; +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%P' +217 ."MFBPCR"\ATV.dial :AIV F*y I +43 h I F*)% h % ."MFBPCR"\AS.seek 0.469ml F*6' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%X' +2609 h ' .t\PSV.dial PSR P%? +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%R' +740 h ' ."MFBPCR"delay 5 seconds +500 F*T9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*V n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%+ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%z' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%N' +2610 h ' .t\PSV.dial PSR P%p +113 h P%b% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%2  +38 h  .t\PS.seek 0ml P%Y' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%H' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%! +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %:'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %&' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*Z I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *''V +850 h ' .t\PSV.dial :air P%"  +113 h  .t\PS.seek 2.5ml P%^' +2610 h ' .t\PSV.dial :mixloop P%r +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%m  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %1' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *]'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*A I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*E' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*$ n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*3% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *~' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*4  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b * '_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'Q  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  '0  +38 h  +113  .t\CBV.dial :waste1 C'( @ +37 h @ .t\CS.seek 0ml C'/'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*_'ݏ +110 h '4 .t\CSV.dial CSR C'}  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*U  +22 h F*L% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*v'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*a n +108 h n F*j% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'`' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'-'4 +2647 h '4 C'l& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'w( +416 h ( M&#& h &ӷ M&@% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &|'η +90 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'O& h & M&<% h %\  A(8% h %X A(\ . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (k( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (e& h &  M&S& h &- M&t% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &{( +166 h ( M&B& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(D& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(c' +196 h ' A(s& h &  M&I& h & ."WCR"\Elbow.seek 2 M&q( +6 h ( M&g& h & A(.& h &  ."WCR"\Forearm.seek 2 + 300 counts M&C'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +59 h '&0 ."MFBPCR"delay 3 seconds +91 h / ."downElevator"\SE.seek :down A(;'X M&F& h & M&y% h %; ."WCR"\Forearm.seek :retract M&)'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +13 ."MFBPCR"\ATV.dial :waste2 F*M % +25 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &?' +18 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +73 h ' ."WCR"\CC.seek :closed C'X(W +416 h (W ."WCR"Hand.open M&1 +71 h 'R ."MFBPCR"\ATV.dial :air2 F*i  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *T' +57 h 1 M&V% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +6 h + S)+% h % C'R& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'%  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*u' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*K I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *O' +4416 h ' ."MFBPCR"\ATV.dial :waste F*< n +128 h n ."MFBPCR"\AS.seek :empty F*8'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*\ % +43 h % F*e% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*|% h % ."MFBPCR"\AS.seek 0.579ml F*B' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*I% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*g' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F**'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*{ % +107 h % F*,% h %V ."MFBPCR"\AS.seek 0.59ml F* ' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*P n +107 h n ."MFBPCR"\ASV.dial :wasteR F*M% h %  ."MFBPCR"\AS.seek 0.09ml F*c' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*C  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*q I +86 h I F*;% h % ."MFBPCR"\AS.seek 0.12ml F*)' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +22 h % F*w% h % ."MFBPCR"\AS.seek 0.13ml F*i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*V% h % ."MFBPCR"\AS.seek 0.23ml F*+' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*%'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +21 h F*k% h %X ."MFBPCR"\AS.seek 0.24ml F*6' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*p n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*b  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*z @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*!'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*T'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *Z'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*"'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*&% h %(A ."MFBPCR"\AS.seek 0.6ml F*r'\  +1032 h '\  F*:% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *d' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*' % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3472 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep0309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F*1'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +22 h ."MFBPCR"delay 2 seconds +200 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*L  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*_  +43 h  ."MFBPCR"\ASV.dial :waste1 F*v  +64 h ."MFBPCR"\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254660 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'[  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'l @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'#% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'>' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'@  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'<% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'\ ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'O @ +75 h @ +50 S)8% h % ."WCR"\CBV.dial between CSV and waste2b C't  +95 h  C'|% h % ."WCR"\CSV.dial between air and CTV C'S ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'B  'D  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 's'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'I  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'.  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'g% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'*' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'{ @ +76 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'P% h % ."WCR"\CSV.dial between air and CTV C'M ` +56 h ` ."WCR"\CS.seek 7.288ml C' ' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'C  ."WCR"\CSV.dial CTV C'q  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ';'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'c  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'w  +151 h C')& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(V% h %X A(i. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (R( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&+& h &ӷ M&?% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +143 h ( M&%& h & A(p& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &b' +89 h ' A(6& h & ."WCR"\SE.seek 12.71 Pucks below Top Plate A(2' +136 h ' M&N& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'Y +26 h 'Y ."WCR"\Elbow.hold M&Y, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(!- M&$&.,(\ #b h -+ ."WCR"\SE.seek 13.04 Pucks below Top Plate A( '" +27 h '" M&% h %Y A(T& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'υ +20 h 'υ M&Z& h & A(&& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &"' +22 h ' ."WCR"\SE.seek 14.04 Pucks below Top Plate A(:' +37 h ' M&r& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&'(ӷ +143 h (ӷ M&d& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'H( +415 h ( A(^& h & A(1& h & M&E& h &ӷ M&$% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&4& h & A(o& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &3'Y +92 h 'Y ."WCR"\Elbow.hold M&~, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&]% h %Y A(J& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&h'υ +20 h 'υ M&Q& h & A((& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A(7% h % ."downElevator"\SE.seek :down A('X +100 (}% h %v M&0% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +23 h 'X ."downElevator"\SE.coast A(5 +67 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&1 +2 h 1 M&L& h & M&U% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&=/ +150 h / M&a& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'& h &W .tHand.open M +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'@& h &W C'% h % .t\CSV.dial CSR C'>  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'K% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'u'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'\ & h &W C'O% h %4 .t\CSV.dial :flush C'8  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 't'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'S% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CTV C'B  .t\CTV.dial CSV C'D  +38 h  +112  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'I'4 +766 h '4 #t\Flushing lower manifold's waste2b port C's& h &W C'.% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'g'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'*% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CTV C',  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'y  +75 h  .t\CS.seek 0ml C'P'4 +766 h '4 #t\Flushing upper manifold C'M& h &W C'F% h %4 .t\CSV.dial :flush C'q  +75 h  .t\CS.seek 5ml C'C'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'c% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CBV C'w .t\CBV.dial CSV C')  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'V'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'i'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'+'4 +506 h '4 C'?& h &W C'%% h %4 .t\CSV.dial :flush C'p  +38 h  .t\CS.seek 3ml C'6'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'N% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'!'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C'f'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'Z& h &W C'&% h %4 .t\CSV.dial CSR C'r  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'b'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'k' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'2 +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'd' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'm  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'"% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'Y'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  '  +38 h  +112  .t\CBV.dial :waste1 C'^ @ +38 h @ .t\CS.seek 0ml C'1'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'E  +37 h  C'$% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '4'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C''  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C']  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Q'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +709 h ' ."connect"\CSV.dial CBV C'7 .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'A  +151 h .t\CS.seek 0ml C'}'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +506 h '0 .t\CSV.dial :waste C'/ +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'L& h &W C'U% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C': @ +38 h @ C'_% h %p .t\CS.seek 7ml C'v'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'n +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'R'0 +506 h '0 .t\CSV.dial :waste C'~ +38 h .t\CS.seek 0ml C'='4 +507 h '4 C'a& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'j'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C'-% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'#'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'@% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'K  +37 h  +113  .t\CBV.dial :waste1 C'< @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'O% h %4 .t\CSV.dial :flush C'8  +75 h  .t\CS.seek 5ml C'u'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'S% h %p .t\CS.seek 7ml C't'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'B  +38 h  .t\CBV.dial :waste2b C'D  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'e& h &W C's% h %4 .t\CSV.dial CSR C'.  +38 h  C' % h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'I'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'W' +416 h ' ."connect"\CSV.dial CBV C'* .t\CBV.dial CSV C'g  +38 h +75 .t\CTV.dial :intake C',  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'y' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'{  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +607 h '4 .t\CSV.dial CSR C'F  +113 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'P'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C' ' +1372 h ' .t\CSV.dial CBV C'c +113 h .t\CS.seek 0ml C'C'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&V' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'i( +416 h ( M&?& h &ӷ M&%% h % .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &+(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&1 +150 h 1 M&!% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&Z1 +2 h 1 M&&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &r(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.seek :garage M&b'η +91 h 'η .tHand.close M&2/ +150 h / M&& h &- M&k% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'Y(W +416 h (W C'X& h &W .tHand.open M&1 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'$% h %4 .t\CSV.dial CSR C'1  +113 h  C'4% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ''% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'o'[ +463 h '[ .t\CSV.dial :waste C'] +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +2010 h '4 #t\Purging external Intake C'& h &W C'Q% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'A .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'(  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'}'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'/  +76 h #t\Flushing upper manifold C'0& h &W C'L% h %4 .t\CSV.dial :flush C'U  +75 h  .t\CS.seek 5ml C'3'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C':% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h +113 h .t\CTV.dial :air .\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'~'4 +507 h '4 C'R& h &W C'a% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +37 h @ C'l% h %0 .t\CS.seek 5ml C'-'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'j'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&H& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&>& h &ӷ M&K% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &<(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&81 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Completed [mbaPhase] begun 06:00:15 #\Preparing to sleep until Tue Sep 04 06:00:00 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST04-Sep-18 #\Continuing at 06:00CEST04-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:28:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST04-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0309h25ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*B  +21 h ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*{  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +65 h  F*F% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *q'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*?% h % ."MFBPCR"\AS.seek 0.81ml F*%'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*N'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*!'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep0311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*"% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *1'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*4  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*C' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*'  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*o  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*( n +21 h n ."MFBPCR"delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.6ml F*/'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep0311h25ml.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*=% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*B'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep0313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*M% h %V ."MFBPCR"\AS.seek 0.5ml F*P'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*[  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*? I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*!  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*Z'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*& n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*k  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep0313h25ml.pcr F*r% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F*X % +87 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*4'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*''n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*/  +31 h  ."MFBPCR"\ATV.dial :air3 F*} % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +21 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +21 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep0316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*H  +10 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*> n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*O% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*S I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F*9' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*D % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*B'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +108 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*.'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*M % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep0316h25ml.pcr F*F% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*c % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*N  +65 h  F*w% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *!'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*2  +31 h  ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep0318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*o  +11 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*Q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*/  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*}  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*U n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*:' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*z  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*v  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*3'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*~'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*= n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep0318h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\ 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*#'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*D  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F**  +32 h  ."MFBPCR"\ATV.dial :air3 F*I % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *{' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +21 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*c% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*P  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep0320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ARV.dial :carrier F*V  +11 h  F*!% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :air1 F*f  +21 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*b I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*T % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*m  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +22 h  ."MFBPCR"delay 7 seconds +700 F*^% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*1  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*4% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*'  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*J % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep0320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*] @@T ."MFBPCR"\ASV.dial :SPEdelivery F*Q  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*(  +151 h F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b */' +423 h ' ."MFBPCR"\ATV.dial :ASV F*} n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*0% h % ."MFBPCR"\AS.seek 0.62ml F*U'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*h n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *z'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*n'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *R' +423 h ' ."MFBPCR"\ATV.dial :air3 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +21 h F*3% h % ."MFBPCR"\AS.seek 0.71ml F*=' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h % H ."MFBPCR"\AS.seek 0.735ml F*-' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F* ' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*j n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*@% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %  ."MFBPCR"\AS.seek 0.604ml F*\ '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*O% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*#' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +86 h n ."MFBPCR"\ASV.dial :wasteR F*`  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*9 % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*B% h % ."MFBPCR"\AS.seek 0.58ml F*e' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h % q ."MFBPCR"\AS.seek 0.605ml F**'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +21 h F*g% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AS.seek 0ml F*{'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *F'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*q  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*c n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *W'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*P n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +202 h 'R ."MFBPCR"\SPE.coast F*[  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*?  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *;' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*!  +64 h  F*N% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*& n +65 h n F*p% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*2 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Z  +43 h  F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*T% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *r'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*+ n +65 h n F*m% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *X' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*" I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*^  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *E'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*$ n +151 h n ."MFBPCR"\AS.seek 0ml F*1'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*4  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*o'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*A% h % ."MFBPCR"\AS.seek 0.97ml F*7'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*} I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *0'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*z  +43 h  F*:% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *v' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *n' +9988 h ' ."MFBPCR"\ATV.dial AIV F*_ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*R' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +129 h n ."MFBPCR"\AS.seek 0ml F*3'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*~  +22 h ."MFBPCR"\ATV.dial :air3 F*= % +43 h % F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*j% h %Z ."MFBPCR"\AS.seek 0.81ml F*@'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +64 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*# % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*I  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' @CEST1536012000.87 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *W'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*) % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2133939 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )? h  C' '  h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *;  h  .t\SPE.configure SPEconfig F*\ 2 *%% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:29:35CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #6) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&1 +150 h 1 M&}& h &_ M&7% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &h'η +91 h 'η .tHand.close M&U/ +150 h / M&z& h &- M&:% h %η .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +264 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &a' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'R& h &W .tHand.open M&31 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'l& h &W C'% h %4 .t\CSV.dial CSR C'-  +150 h  C'j% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'H'[ +463 h '[ .t\CSV.dial :waste C'@ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'Y  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'> .t\CBV.dial CSV C'd  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'8'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'O% h %4 .t\CSV.dial :flush C'\  +75 h  .t\CS.seek 5ml C'`'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +37 h @ C'% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'9 +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'u +37 h .t\CS.seek 0ml C'B'4 +507 h '4 C't& h &W C'% h %4 .t\CSV.dial :flush C'.  +38 h  .t\CS.seek 3ml C's'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'I @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'*'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +507 h '0 .t\CSV.dial :waste C', +75 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M&c' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'[( +416 h ( M&P& h &ӷ M&)% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &|(- +264 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:29:40CEST30-Aug-18\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:29:35CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C']& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&?% h %\  A( % h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (;& h &  M&%& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &w( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*! % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *N' +160 h ( M&V& h & A(&& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &p' +89 h ' A(& h &  .t\SE.seek 5.71 Pucks below Top Plate A(2' +205 h ' A(G& h &  M&f& h & .t\Elbow.seek 2 M&Z( +7 h ( M&b& h & A(T& h &  .t\Forearm.seek 2 + 300 counts M&k'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down A(m'X M&6& h & M&r% h %; .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.seek CC M&$(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'J(W +415 h (W .tHand.open M&Q1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)1% h % C'A& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% ."airStroke"\CS.seek 0ml C''4 h % ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)K  +6 h '4 ."airStroke"\CSV.dial :air C' @ +76 h @ ."airStroke"\CS.seek 10ml C'' +144 h  .t\CTV.dial :air C'O  +75 h .tdelay 15 seconds +690 h ' +810 .t\SSV.dial :spit,avoiding: :exhaust S)\ +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'8  .t\SS.seek 0ml S)#' +6 h ' .t\SSV.dial CBV,avoiding: :exhaust S)  +144 h  +7  S)`% h % .t\CBV.dial between SSV and waste2 C'9  +19 h  C'S% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'  +37 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'u'4 +2610 h '4 C'D% h %4 S)t% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C' @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X9 h 9d +100 C'9 h 9d +100 C'K9 h 9'd +100 C' 9 h 9>d +100 C'O9 h 9Td +100 C'9 h 9kd +100 C'\ 9 h 9d +100 C'89 h 9d +100 C'9 h 9d +100 C'`9 h 9d +100 C'99 h 9d +100 C'S9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'9 h 96d +100 C'D9 h 9Ld +100 C't9 h 9bd +100 C'9 h 9xd +100 C'#9 h 9d +100 C'B9 h 9d +100 C'I9 h 9d +100 C'e9 h 9d +100 C'.9 h 9d +100 C's9 h 9d +100 C'*9 h 9d +100 C',9 h 9(d +100 C'{9 h 9>d +100 C'g9 h 9Td +100 C'F9 h 9id +100 C'u9 h 9d +100 C'q9 h 9d +100 C'y9 h 9d +100 C' 9 h 9d +100 C'=9 h 9d +100 C'9 h 9d +100 C')9 h 9  d +100 C'<9 h 9 d +100 C'v9 h 9 *d +100 C'c9 h 9 ?d +100 C'\\9 h 9 Td +100 C'9 h 9 j d +100 C'P9 h 9 $d +100 C'M9 h 9 (d +100 C'W9 h 9 ,d +100 C'9 h 9 0d +100 C'?9 h 9 4d +100 C' 9 h 9 8d +100 C'9 h 9 'Y +26 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(K- h -+n M&$&.,(\ #b .t\SE.seek 14.04 Pucks below Top Plate A( ' +27 h ' M&O% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &\ 'υ +20 h 'υ M&8& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &`' +23 h ' .t\SE.seek 15.04 Pucks below Top Plate A(9' +36 h ' M&S& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'B( +416 h ( A(I& h & A(e& h & M&.& h &ӷ M&s% h % .t\Forearm.seek :retract M&*'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &,( +143 h ( M&{& h & A(g& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &F'Y +92 h 'Y .t\Elbow.hold M&u, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &q1 +150 h 1 M&y% h %Y A( & h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &='υ +20 h 'υ M&& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +22 h ' A(v% h % ."downElevator"\SE.seek :down A(c'X +100 (\\% h %- M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +13 h 'X ."downElevator"\SE.coast A(5 +77 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'M @ +19 h @ .t\CS.seek 10ml C'W' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'? { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&& h & M&;% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&!'η +91 h 'η .tHand.close M&]/ +150 h / M&&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'G& h &W .tHand.open M&+1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%b +151 h P%6% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%w' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%p  +38 h  .t\PS.seek 0ml P%r' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%^' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'2  P%k  h +75 .t\PSV.dial :mixloop P%Z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%X  h .t\PS.seek 0ml P%m' +2610 h ' .t\PSV.dial :air P%Q  +37 h  .t\PS.seek 2.5ml P%[' +2610 h ' .t\PSV.dial :mixloop P%1 +38 h .t\PS.seek 0ml P%A' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%4' +2610 h ' .t\PSV.dial :mixloop P%} +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +6 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'"( +416 h ( M&:& h &ӷ M&% h % .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &N(- +265 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &_'η +91 h 'η .tHand.open M&(1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'3& h & M&~% h %\  A(l% h %X A(L. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (j( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M& & h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &@( +166 h ( M&H& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +89 h ' A(& h &  .t\SE.seek 4.71 Pucks below Top Plate A(d' +215 h ' A(K& h &  M&O& h & .t\Elbow.seek 2 M&( +6 h ( M&8& h & A(& h &  .t\Forearm.seek 2 + 300 counts M& '; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&-% h %; .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.seek CC M&>(ӷ +124 h 'X ."downElevator"\SE.coast A(5 +19 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\ ' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&`1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'J  +19 h  .t\CS.seek 5ml C'I'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'e' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'.'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%s  +38 h  .t\PS.seek 2.5ml P%{' +2610 h ' .t\PSV.dial :mixloop P%g +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%q  +150 h  .t\PS.seek :empty P%y' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C') @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'v  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'* ` +18 h ` .t\CSV.dial :air C', @ +38 h @ .t\CS.seek 10ml C'\\' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'M'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'?  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(% h %X A(;. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (F( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (=& h & M&9& h &ӷ M&<% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&x& h & A(c& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &a' +90 h ' A(R& h & .t\SE.seek 14.71 Pucks below Top Plate A(u'] +116 h '] M&]& h & A(&& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&P, +7 h , .t\Elbow.reconfigure TightElbow A(!- M&$&.,(\ #b h -+] .t\SE.seek 15.04 Pucks below Top Plate A(|' +26 h ' M&f% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&B& h & A(G& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &+' +22 h ' .t\SE.seek 16.04 Pucks below Top Plate A(%' +36 h ' M&b& h & M&6% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +89 h '\  .t\Elbow.seek CC M&p(ӷ +143 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&r' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'2( +416 h ( A(Z& h & A(X& h & M&^& h &ӷ M&Q% h % .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &1( +142 h ( M&[& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &A'Y +93 h 'Y .t\Elbow.hold M&}, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &41 +150 h 1 M&i% h %Y A(0& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&/'υ +20 h 'υ M&z& h & A(V& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &E' +23 h ' A(T% h % ."downElevator"\SE.seek :down A(:'X +100 (% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +3 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%'% h % .t\PS.seek 0.499ml P%(' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%7 +37 h .t\PRV.dial :air P%U  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%3  +38 h .t\CBV.dial AIV C'~  +37 h .t\PRV.dial between CBV and air P%l  +19 h  .t\PRV.dial :air P%L  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %_' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&1 +2 h 1 M&& h & M&N% h %\  .t\Elbow.seek FlushPuck::Garage M&C(- +166 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&8'η +91 h 'η .tHand.close M&/ +150 h / M&S& h &- M&% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +265 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +90 h ' .t\CC.seek :closed C' (W +416 h (W C'd& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  C'"& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%` @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%J% h % .t\PS.seek 1.499ml P%\ ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%j' +1094 h ' .t\PSV.dial :air P%W  +38 h  .t\PS.seek 0ml P%I' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%e +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%s  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %g'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%q' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%{  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%)  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %v' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%* +76 h .t\PTV.dial :PRV,avoiding: :puck P%, @ +75 h @ .t\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%\\' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%?' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%;  +75 h P%=% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %9' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'< & +50 h & .t\AWV.select :waste C' +6 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %c'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'R { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %>' +1313 h ' .t\PSV.dial :air P%]  +38 h  .t\PS.seek 2.5ml P%&' +2609 h ' .t\PSV.dial :PML P%! +38 h .t\PS.seek 0ml P%f' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'B& h &W F*G @@T .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass .\PSV.dial PSR F*#  P%b +22 h  ."MFBPCR"delay 20 seconds +91 h P%6% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%a' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%w  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%|' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%P' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*k  +22 h ' .t\PSV.dial PSR P%5 +21 h  F* % h %6 ."MFBPCR"\AS.seek 0.329ml F*Z'ͯ +92 h P%X% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%^' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*Q % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *['R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%r' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*p  +22 h  F*4% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%0 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +217 ."MFBPCR"\ATV.dial :AIV F*V I +43 h I F*T% h % ."MFBPCR"\AS.seek 0.469ml F*m' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%1 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%A% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%/' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*'9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*E n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*:% h % ."MFBPCR"\AS.seek 0.041ml F*F'% +336 h ' .t\PSV.dial PSR P%y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%7' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%3' +2610 h ' .t\PSV.dial PSR P%~ +113 h P%l% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%L' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%(' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%N  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %K' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*O I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml .t\PS.hold F*#,,'T20 b *}'V P%+ h '7 +80 +4C !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg",Processing Syringe seek to 0ml Aborted!:@checkpoint: omitted #-> exit ."MFBPCR"\SPE.coast .t\AS.stop F*S  *5 h  '8 !to:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"4Exiting with Collection Clamp holding FlushPuck #m\EXIT @1535635828.83CEST @1535636590.68CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:43:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:43:35, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:43:39, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:43:44CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:43:39, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & .t\SE.seek :down A( 'X +7 h 'X .t\SE.coast M&% A(5 h %\  !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"User Abort!!:@checkpoint: omitted #-> exit .\AS.stop F*5 h ' ) #m\EXIT @1535636628.24CEST @1535636661.27CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> delayUntill '08:00 30-aug-2018' !o:NoMethodError :bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message3undefined method `delayUntill' for main:Object: name:delayUntill: args["08:00 30-aug-2018 #-> delayUntil '08:00 30-aug-2018' .delayUntil "08:00:00.00CEST30-Aug-18" @CEST1535608800.00 #-> execute 18tundeploy.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"|compile error\ (ESP):3: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeploy.rb\ ^ #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:44:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 08:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:46:12, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 08:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:46:16, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:46:20CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:46:16, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)!  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C'!9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (!& M&I& h & &ӷ ."raiseStack"\SE.seek :upperLimit A(E'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&&& A(A'X h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %Y A(p& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P% % h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &!' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*!'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  'H  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&x% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 * % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug3011h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  'q  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A( 'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&M'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&H% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C':% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'&  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'A  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'N  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'M'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'q% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'H& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 08:00:26 #\Preparing to sleep until Sat Sep 01 08:00:26 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 08:00CEST01-Sep-18 #\Continuing at 08:00CEST01-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:47:43, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 08:00CEST01-Sep-18" +235134 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi !; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118aug3011h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18aug3013h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +107 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*H  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18aug3013h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*^  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18aug3015h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*/  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*('\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*4 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18aug3015h25ml.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*<  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*B  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*L % +86 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18aug3018h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +10 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*d n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*| I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18aug3018h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +21 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug3020h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*l n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*s  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +22 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218aug3020h25ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *;' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18aug3022h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *z'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*4 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*<  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +22 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 @CEST1535669496.94 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18aug3022h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*B @@T ."MFBPCR"\ASV.dial :SPEdelivery F*%  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*F  +151 h F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *S' +423 h ' ."MFBPCR"\ATV.dial :ASV F*L n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*H% h % ."MFBPCR"\AS.seek 0.62ml F*l'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*M n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *s'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*f' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % H ."MFBPCR"\AS.seek 0.735ml F*e' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +21 h F*W% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*q% h % zG ."MFBPCR"\AS.seek 0.365ml F*m' +7161 h ' ."MFBPCR"\ATV.dial :waste F** n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*C% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +21 h F*`% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*"' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*| n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*b'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *n'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*u' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*;'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*? n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *r'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +202 h 'R ."MFBPCR"\SPE.coast F*V  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +42 h  F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *~': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*1  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *7'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*8 n +65 h n F*$% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *k': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*z  +64 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *&'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Y n +65 h n F*<% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*2  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *A'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*J n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*0 % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*)% h % ."MFBPCR"\AS.seek 0.96ml F*I'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %0 ."MFBPCR"\AS.seek 0.985ml F*N' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.97ml F*X'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*L I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *M'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Z' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*[% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*+  +21 h ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*\\% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *r'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*{ % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1535781626.57 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' '@ h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)/% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% C'4  h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &k'\ h ."rdySampler"\CSV.dial :waste1 C'Y h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)< h  S)&  +37 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %'' +21 h '\  ."closeHand"Hand.close M&/ .t\Elbow.jog 1500 M&2* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:48:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&N1 +150 h 1 M&L& h &_ M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &](- +166 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &M'η +91 h 'η .tHand.close M&l/ +150 h / M&s& h &- M& % h %η .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'P% h %4 .t\CSV.dial CSR C'e  +151 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C'q +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ':'4 +2010 h '4 #t\Purging external Intake C'& h &W C'm% h %4 .t\CSV.dial :flush C',  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h '9  h +75 .t\CTV.dial :intake C'D  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'=  +75 h #t\Flushing upper manifold C'd& h &W C'`% h %4 .t\CSV.dial :flush C'6  +76 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C'b% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CBV C'O .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'p +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'n& h &W C'K% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'a'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'u% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C'j'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&w& h &ӷ M&{% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &#(- +265 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +90 h 'η .tHand.open M&.1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:48:43CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:48:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'B& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(@% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&/% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &c( +7 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F*1 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +159 h ( M&~& h & A(8& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +90 h ' A(7& h &  .t\SE.seek 17.71 Pucks below Top Plate A( '* +86 h '* A(G& h &  M&(& h & .t\Elbow.seek 2 M&3( +7 h ( M&& h & A(>& h &  .t\Forearm.seek 2 + 300 counts M&4'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down M&z& A(<'X h & M&&% h %; .t\Forearm.seek :retract M&'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&J(ӷ +142 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'U(W +416 h (W .tHand.open M&%1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'0& h &W ."airStroke"\CSV.dial :waste S)% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'\ '4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9d +100 C'59 h 9d +100 C'Y9 h 91d +100 C'z9 h 9J d +100 C'&9 h 9bd +100 C'A9 h 9zd +100 C'c9 h 9d +100 C'$9 h 9d +100 C'29 h 9 d +100 C'R9 h 9$d +100 C'49 h 9(d +100 C' 9 h 9 ,d +100 C'<9 h 9$0d +100 C'39 h 9<4d +100 C'9 h 9S8d +100 C'V9 h 9kd +100 C']9 h 9Td +100 C'_9 h 9kd +100 C'F9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C's9 h 9d +100 C'X9 h 9d +100 C'i9 h 9d +100 C'P9 h 9 d +100 C'e9 h 9 d +100 C'Z9 h 96d +100 C'Q9 h 9Ld +100 C')9 h 9bd +100 C'g9 h 9xd +100 C'W9 h 9d +100 C'q9 h 9d +100 C'\\9 h 9d +100 C'*9 h 9d +100 C'm9 h 9d +100 C',9 h 9d +100 C'99 h 9d +100 C'9 h 9(d +100 C'D9 h 9>d +100 C'+9 h 9Td +100 C'h9 h 9id +100 C':9 h 9d +100 C'C9 h 9d +100 C'd9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'b9 h 9d +100 C'=9 h 9  d +100 C'"9 h 9 d +100 C'E9 h 9 *d +100 C'|9 h 9 ?d +100 C'69 h 9 Td +100 C'O9 h 9 j d +100 C'p9 h 9 $d +100 C' 9 h 9 (d +100 C'n9 h 9 ,d +100 C'K9 h 9 0d +100 C' 9 h 9 4d +100 C'9 h 9 8d +100 C'u9 h 9 'p +1372 h 'p .t\CSV.dial CTV C'5  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'Y @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'z  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'& @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'A'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'c +113 h .t\CBV.dial CSV C'$  +19 h C'2% h %4 .t\CS.seek 5ml C'R'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'4  +38 h C' % h %p .t\CS.seek 7ml C'<'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'3 @ +38 h @ .t\CS.seek 10ml C'' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'V ` +131 h ` #tLysing Complete C'%& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(0% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (L( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&j% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&H& h & A('& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\ ' +90 h ' A(S& h & .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': M&l& h & A(N& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b & 'Y +25 h 'Y .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(M- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(' +26 h ' M&]% h %Y A(_& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&F'υ +21 h 'υ M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(X' +36 h ' M&i& h & M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +89 h '\  .t\Elbow.seek CC M&Z(ӷ +143 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'q( +416 h ( A(\\& h & !to:Puck::Warning:bt["7/home/esp/esp2/utils/puckmoves.rb:223:in `giveup!'"6/home/esp/esp2/utils/puckmoves.rb:405:in `unload'"4/home/esp/esp2/utils/puckmoves.rb:358:in `load'"8/home/esp/esp2/utils/puckmoves.rb:882:in `loadPuck'"//home/esp/esp2/protocol/sh1.rb:39:in `sh1'"//home/esp/esp2/protocol/MFB.rb:43:in `mfb'":/home/esp/esp2/protocol/utilities.rb:67:in `protocol'"//home/esp/esp2/protocol/MFB.rb:41:in `mfb'":/home/esp/esp2/mission/18tundeploy.rb:70:in `coreNEG'"./home/esp/esp2/mission/18tundeploy.rb:111"7/home/esp/esp2/utils/commskel.rb:81:in `runAction'"//home/esp/esp2/utils/mission.rb:71:in `at'"./home/esp/esp2/mission/18tundeploy.rb:110"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"./home/esp/esp2/mission/18tundeploy.rb:104"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):4:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"BRetaining claim on Arm because Hand is likely holding a puck! !o:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"User Abort!!:@checkpoint: omitted #-> exit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"0Exiting with Hand holding Puck.from 2, :sh1 #m\EXIT @1535636951.43CEST @1535637685.36CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> delayUntil '08:00 30-08-18' .delayUntil "08:00:00.00CEST18-Aug-30" @CEST1913263200.00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:01:33CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 08:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:02:30CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 08:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:02:34CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:02:39CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:02:34CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'S9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'P9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & M&I& h & &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(E'Z .t\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(R& h & M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(P& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'\  P%  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down M&&& h & A(A'X M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%P +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'S ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %Y A(p& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%2  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %P'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%S +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*O @@T .t\AIV.dial :bypass F*t  .t\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%2' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'P'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'S'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&x% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *2'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt130aug1811h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  'q  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(2& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(1& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'P& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&S% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C':% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV C'y  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'&  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'A  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X 'N  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'P +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'Q& h &W C'S% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'q% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  'C  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C' '4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'P  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 08:00:26 #\Preparing to sleep until 15/8/18 06:00AM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 15/8/18 06:00AM !o:ArgumentError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"argument out of range #-> exit #m\EXIT @1535637912.13CEST @1535638118.37CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> exit #m\EXIT @1535638133.87CEST @1535638206.33CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> delayUntil '12:00 13-sep-18' .delayUntil "12:00:00.00CEST13-Sep-18" @CEST1536832800.00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:10:14CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 12:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:11:09CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 12:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:11:13CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q  'R h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:11:17CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:11:13CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'Q9 h 9|d +100 C'R9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & ."raiseStack"\SE.seek :upperLimit M&I& A(E'Z h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(Q& h & M&R& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down A(&'X M&A& h & M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'Q' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'R'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %Y A(p& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 .\Forearm.seek 1 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +21 h 'υ M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&A% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&&1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%Q +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%R' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*&' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'Q% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'S'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*R n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & h & A('X M&x% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*A'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1315h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*Q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'q  ."WCR"\CSV.dial CTV C':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&A& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&&& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Q1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&M'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&H% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&R& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'q% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV C'y  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ 'c  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'A  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV C'! .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'&  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'Q'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N  'X +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'M'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'R& h &W C'P% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C':% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &A(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'&& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'Q& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'H& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'R% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 12:00:26 #\Preparing to sleep until 06:00 15-sep-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00 15-sep-18 #\Continuing at 06:00CEST15-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:12:43CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST15-Sep-18" +235134 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi !; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1315h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1317h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*A'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +107 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*H  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1317h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*^  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1319h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*/  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*('\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*4 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1319h25ml.pcr F*A% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*<  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *&'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*B  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*L % +86 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1322h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +10 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*d n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*| I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CEST1536876607.17 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1322h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*{% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +31 h  ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +21 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1400h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*l n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*s  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +22 h  ."MFBPCR"delay 7 seconds +700 F*R% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1400h25ml.pcr F*q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *;' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1402h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *z'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*4 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*<  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +22 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1402h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*B @@T ."MFBPCR"\ASV.dial :SPEdelivery F*%  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*F  +151 h F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *S' +423 h ' ."MFBPCR"\ATV.dial :ASV F*L n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*H% h % ."MFBPCR"\AS.seek 0.62ml F*l'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*M n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *s'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*f' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % H ."MFBPCR"\AS.seek 0.735ml F*e' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +21 h F*W% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*:% h % zG ."MFBPCR"\AS.seek 0.365ml F*m' +7161 h ' ."MFBPCR"\ATV.dial :waste F** n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*q % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*C% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +21 h F*`% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*"' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*| n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*b'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *n'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*u' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*;'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*? n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *r'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +202 h 'R ."MFBPCR"\SPE.coast F*V  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +42 h  F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *~': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*1  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *7'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*8 n +65 h n F*$% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*! I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *k': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*z  +64 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *A'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Y n +65 h n F*<% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*2  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *&'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*J n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*0 % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*)% h % ."MFBPCR"\AS.seek 0.96ml F*I'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %0 ."MFBPCR"\AS.seek 0.985ml F*N' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.97ml F*X'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*L I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *M'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Z' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*R n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*[% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*+  +21 h ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*\\% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *r'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*c % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1536984000.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '@ h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)/% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X  C'z  M&4% h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &k'\ h ."rdySampler"\CSV.dial :waste1 C'Y h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)< h  S)A  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %'' +20 h '\  ."closeHand"Hand.close M&/ .t\Elbow.jog 1500 M&2* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:13:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&N1 +150 h 1 M&L& h &_ M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &](- +166 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &M'η +91 h 'η .tHand.close M&l/ +150 h / M&s& h &- M& % h %η .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &R' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'P% h %4 .t\CSV.dial CSR C'e  +150 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C': +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'q'4 +2009 h '4 #t\Purging external Intake C'& h &W C'm% h %4 .t\CSV.dial :flush C',  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h '9  h +75 .t\CTV.dial :intake C'D  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'=  +75 h #t\Flushing upper manifold C'd& h &W C'`% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'E'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'b% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'p +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'n& h &W C'K% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'a'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'u% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&w& h &ӷ M&c% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &#(- +264 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&.1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:13:44CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:13:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'B& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(@% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&/% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &{( +6 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F*1 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +160 h ( M&~& h & A(8& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +89 h ' A(7& h &  .t\SE.seek 17.71 Pucks below Top Plate A(!'* +87 h '* A(G& h &  M&(& h & .t\Elbow.seek 2 M&3( +6 h ( M&& h & A(4& h &  .t\Forearm.seek 2 + 300 counts M&z'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down M&>& A(<'X h & M&A% h %; .t\Forearm.seek :retract M&'\ +87 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .t\Elbow.seek CC M&J(ӷ +143 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'U(W +416 h (W .tHand.open M&%1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'0& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)\ % C''4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9J d +100 C'A9 h 9bd +100 C'&9 h 9zd +100 C'{9 h 9d +100 C'$9 h 9d +100 C'29 h 9 d +100 C'Q9 h 9$d +100 C'z9 h 9(d +100 C'!9 h 9 ,d +100 C'<9 h 9$0d +100 C'39 h 9<4d +100 C'9 h 9S8d +100 C'V9 h 9kd +100 C']9 h 9Td +100 C'_9 h 9kd +100 C'F9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C's9 h 9d +100 C'X9 h 9d +100 C'i9 h 9d +100 C'P9 h 9 d +100 C'e9 h 9 d +100 C'Z9 h 96d +100 C'R9 h 9Ld +100 C')9 h 9bd +100 C'g9 h 9xd +100 C'W9 h 9d +100 C':9 h 9d +100 C'\\9 h 9d +100 C'*9 h 9d +100 C'm9 h 9d +100 C',9 h 9d +100 C'99 h 9d +100 C'9 h 9(d +100 C'D9 h 9>d +100 C'+9 h 9Td +100 C'h9 h 9id +100 C'q9 h 9d +100 C'C9 h 9d +100 C'd9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'b9 h 9d +100 C'=9 h 9  d +100 C'"9 h 9 d +100 C'E9 h 9 *d +100 C'|9 h 9 ?d +100 C'69 h 9 Td +100 C'O9 h 9 j d +100 C'p9 h 9 $d +100 C' 9 h 9 (d +100 C'n9 h 9 ,d +100 C'K9 h 9 0d +100 C' 9 h 9 4d +100 C'9 h 9 8d +100 C'u9 h 9   +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'A @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'&'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'{ +113 h .t\CBV.dial CSV C'$  +19 h C'2% h %4 .t\CS.seek 5ml C'Q'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'z  +37 h C'!% h %p .t\CS.seek 7ml C'<'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'3 @ +38 h @ .t\CS.seek 10ml C'' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'V ` +132 h ` #tLysing Complete C'%& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(0% h %X A(\ . h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (L( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&j& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&H& h & A('& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(S& h & .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': M&l& h & A(N& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b & 'Y +26 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(M- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(' +27 h ' M&]% h %Y A(_& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&F'υ +20 h 'υ M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(X' +37 h ' M&i& h & M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +89 h '\  .t\Elbow.seek CC M&Z(ӷ +143 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C':( +415 h ( A(\\& h & A(*& h & M&m& h &ӷ M&,% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&D& h & A(+& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &h'Y +92 h 'Y .t\Elbow.hold M&q, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&d% h %Y A(`& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&b& h & A(=& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &"' +22 h ' A(E% h % ."downElevator"\SE.seek :down A(|'X +100 (6% h %1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'n' +6 h ' .t\CSV.dial between air and CTV C'K ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&u1 +2 h 1 M&a& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'[& h &W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%? +151 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%r' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%-' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%B' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%@  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%o  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :mixloop P%~ +38 h .t\PS.seek 0ml P%8' +2609 h ' .t\PSV.dial :air P%1  +38 h  .t\PS.seek 2.5ml P%7' +2610 h ' .t\PSV.dial :mixloop P%( +37 h .t\PS.seek 0ml P%G' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&#& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&>' +90 h ' .tHand.close M&A/ +150 h / .t\CC.seek :open C'4( +416 h ( M&{& h &ӷ M&$% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +265 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Q'η +91 h 'η .tHand.open M&31 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&%% h %\  A(k% h %X A(0. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (\ ( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&j& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &H( +166 h ( M&'& h & A(S& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(& h &  .t\SE.seek 16.71 Pucks below Top Plate A(l'; +96 h '; A(N& h &  M&M& h & .t\Elbow.seek 2 M&]( +6 h ( M&_& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &i/ +150 h / ."downElevator"\SE.seek :down M&P& h & A('X M&% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'e  +150 h  .t\CSV.dial CTV C'U  +19 h  .t\CS.seek 5ml C'\\'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'*' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'm'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%,  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P%+ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Z +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%C  +151 h  .t\PS.seek :empty P%d' +2609 h ' .t\CBV.dial :waste1 C'` @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'b  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'= @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'9 ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'6' +2648 h ' .t\CSV.dial CTV C'O  +37 h  .t\CS.seek 0ml C' '4 +2647 h '4 .t\CBV.dial :waste1 C'h @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'K  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(u% h %X A(a. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&X& h &ӷ M&"% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +143 h ( M&x& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &|' +89 h ' A(& h & .t\SE.seek 2.71 Pucks below Top Plate A(q') +235 h ') M&& h & A( & h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&p, +7 h , .t\Elbow.reconfigure TightElbow A(;- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(f' +27 h ' M&w% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&^'υ +21 h 'υ M&:& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(' +36 h ' M&?& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(& h & M&B& h &ӷ M&o% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &~( +142 h ( M&/& h & A(1& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &8'Y +93 h 'Y .t\Elbow.hold M&(, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &71 +150 h 1 M&#% h %Y A(5& h & .t\Forearm.force -20 .\Forearm.seek 1 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &Y'υ +21 h 'υ M&A& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' A(T% h % ."downElevator"\SE.seek :down A({'X +100 ($% h %  M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%!  +2 A(6 +111 h  P% % h % .t\PS.seek 0.499ml P%3' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%2  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'%  +38 h .t\PRV.dial between CBV and air P%k  +18 h  .t\PRV.dial :air P%0  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %j' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %Q' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C''& h & .tHand.open M&S1 +2 h 1 M&& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& +113 h P%8% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%!' +739 h ' ."MFBPCR"delay 5 seconds +500 F* 9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*t n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*{% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%d +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2609 h ' .t\PSV.dial PSR P%% +113 h P%k% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%0' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%3' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%j' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%S +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%&  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %M' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*_ I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *('V +849 h ' .t\PSV.dial :air P%<  +113 h  .t\PS.seek 2.5ml P%i' +2610 h ' .t\PSV.dial :mixloop P%P +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %z' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*R  +43 h  F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +170 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %4' +618 h ' ."MFBPCR"\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.seek :empty F*)'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*e% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *U' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*Q  +43 h F*F% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *n'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'\  +37 h  C'*% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1625 h ' ."connect"\CSV.dial CTV C'\\  .t\CTV.dial CSV C'g  +38 h  +113  .t\CBV.dial :waste1 C'Z @ +38 h @ .t\CS.seek 0ml C'+'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*D  +22 h ."MFBPCR"delay 3 seconds +300 F*C% h %_ ."MFBPCR"\AS.seek 0.434ml F*b'ݏ +110 h '4 .t\CSV.dial CSR C'=  +37 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'9'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*E% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*O'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*m n +107 h n F*h% h %& ."MFBPCR"\AS.seek 0.092ml F*6'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'u'4 +2647 h '4 C'a& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&X' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'L( +416 h ( M&W& h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &['η +91 h 'η .tHand.open M&?1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'y& h & M&q% h %\  A(r% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (|( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (^& h &  M&& h &- M&}% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &f( +166 h ( M&@& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A(p& h &  M&o& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&1& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&7'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &B/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down M&5& A(/'X h & M&% h %; ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.seek CC M&-(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&#& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &A' +64 ."MFBPCR"\ATV.dial :waste2 F*> % +27 h ' ."WCR"\CC.seek :closed C'8(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +400 h (W ."WCR"Hand.open M&1 +150 h 1 M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*t  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *{' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)d% h % C'!& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'2  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +85 h I F*/% h % ."MFBPCR"\AS.seek 0.12ml F*T' +626 h ' ."MFBPCR"\ATV.dial :air3 F*L % +21 h % F*6% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*t% h % ."MFBPCR"\AS.seek 0.23ml F*d' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h % ."MFBPCR"\AS.seek 0.255ml F*A'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*|% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*k n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*%  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *0'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*S'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*{'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *_'\  +1032 h '\  F*'% h %\  ."MFBPCR"\AS.seek 0.5ml F*<'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*M% h %(A ."MFBPCR"\AS.seek 0.6ml F*P'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*z'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*R% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *N' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*( % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1507h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Q  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*U  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F**% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*,  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Z % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +107 h  ."MFBPCR"delay 7 seconds +700 F*=% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*E  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 F*O  +65 h ."MFBPCR"\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254571 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'"  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'a @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'H @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'l  +95 h  C'W% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'X' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'w  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'.  +37 h  C'q% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'v' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S)r% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'[% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '@  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'p'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'o  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C';  +38 h  C'1% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'f @ +75 h @ +50 S)c% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'~% h % ."WCR"\CSV.dial between air and CTV C'7 ` +57 h ` ."WCR"\CS.seek 7.288ml C'#' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'>  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '/'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'6  +150 h C'T& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&Y% h %\  A(t% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (!( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (2& h & M&d& h &ӷ M&$% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &|( +142 h ( M&A& h & A(k& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(& h & ."WCR"\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' M&V& h & A(0& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y ."WCR"\Elbow.hold M&3, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(S- M&$&.,(\ #b h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(&' +27 h ' M&% h %Y A({& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&''υ +21 h 'υ M&_& h & A(M& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +37 h ' M&P& h & M&R% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +89 h '\  ."WCR"\Elbow.seek CC M&((ӷ +142 h (ӷ M&N& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'j( +416 h ( A(i& h & A(J& h & M&]& h &ӷ M&s% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&Q& h & A(F& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &e'Y +92 h 'Y ."WCR"\Elbow.hold M&U, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &*1 +150 h 1 M&% h %Y A(,& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&\\'υ +21 h 'υ M&\ & h & A(Z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &G' +22 h ' A( % h % ."downElevator"\SE.seek :down A(C'X +100 (=% h % M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +90 h '\  +21 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&`1 +2 A(6 h 1 M&E& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&n(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&h'η +91 h 'η .tHand.close M&K/ +150 h / M&m& h &- M&9% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +265 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C'x(W +415 h (W C'l& h &W .tHand.open M&W1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'w% h % .t\CSV.dial CSR C'X  +38 h  C'?% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '.'[ +463 h '[ .t\CSV.dial :waste C'q +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'v& h &W C'y% h %4 .t\CSV.dial :flush C'r  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'p& h &W C'% h %4 .t\CSV.dial :flush C';  +76 h  .t\CS.seek 5ml C'1'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'B% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CTV C'c  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'~'4 +765 h '4 #t\Flushing upper manifold C'7& h &W C'5% h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +38 h @ C'% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6 'T  +37 h +76 .t\CTV.dial :air C'Y  +150 h .t\CS.seek 0ml C't'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'2 +37 h .t\CS.seek 0ml C'd'4 +507 h '4 C'$& h &W C'A% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C' % h %0 .t\CS.seek 5ml C'V'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'0'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'{'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'_& h &W C'M% h %4 .t\CSV.dial CSR C'P  +151 h  C'R% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'%'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'|' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'N' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'&  +113 h  C'<% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'3'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'z  .t\CTV.dial CSV C'8  +37 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C'J'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C']  +38 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Q'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'(  +75 h  .t\CS.seek 0ml C'*'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C',% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\ 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'='4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'`'4 +507 h '4 #t\Flushing upper manifold C'E& h &W C'% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV C'G .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C'U +38 h .t\CS.seek 0ml C'K'4 +506 h '4 C'm& h &W C'9% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 3ml C'h'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'l +75 h .t\CS.seek 0ml C'W'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C' % h %4 .t\CSV.dial :flush C'w  +37 h  .t\CS.seek 5ml C'X'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C'H% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'q @ +38 h @ .t\CS.seek 0ml C'.'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'v& h &W C'y% h %4 .t\CSV.dial :flush C'r  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'^& h &W C'p% h %4 .t\CSV.dial CSR C'  +37 h  C';% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'o'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B '1  +37 h +76 .t\CTV.dial :intake C'c  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'f  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '7'4 +607 h '4 .t\CSV.dial CSR C'5  +112 h  C'>% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'~'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'#' +1373 h ' .t\CSV.dial CBV C' +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C'( +416 h ( M&$& h &ӷ M&A% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &d(- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&V1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&_1 +2 h 1 M&M& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &P(- +7 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'z& h &W .tHand.open M&81 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C's% h %4 .t\CSV.dial CSR C'J  +113 h  C'Q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '(% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'F'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Purging external Intake C'*& h &W C'\ % h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'Z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'='4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'+  +75 h #t\Flushing upper manifold C'g& h &W C'E% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'e'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV C'\\ .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'G'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'C +38 h .t\CS.seek 0ml C'U'4 +506 h '4 C'!& h &W C'm% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C'D'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'a% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'h'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'l'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'H( +416 h ( M&X& h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &q(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &y'η +90 h 'η .tHand.open M&r1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Completed [coreNEG] begun 06:00:15 #\Preparing to sleep until Mon Sep 17 06:00:15 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST17-Sep-18 #\Continuing at 06:00CEST17-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:15:07CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST17-Sep-18" +235164 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1507h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*c% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*f  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*/  +32 h  ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1509h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*<% h %V ."MFBPCR"\AS.seek 0.5ml F*P'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*i  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +21 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*Q  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*(  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*\ '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*+'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1509h.pcr F*G% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*O  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +87 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *9'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*K% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*h'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1512h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*# n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*$ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*- n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*V  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*k'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*0  +108 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*d  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*%% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1512h.pcr F*P% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*Q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*!% h %m ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1514h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +10 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*v  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*p  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*c' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1514h.pcr F*5% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*#  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*Y% h %Z ."MFBPCR"\AS.seek 0.81ml F*/'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*A% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*V  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*{ % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*<'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*z% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1516h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*F  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*\\% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*O  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +21 h  ."MFBPCR"delay 7 seconds +700 F*m% h %< ."MFBPCR"\AS.seek 0.594ml F*U'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*K n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*u  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1516h.pcr F*w% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*W'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +65 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*T'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1519h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*0% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*{  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*% I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +21 h n ."MFBPCR"delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*(  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1519h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*\  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Z  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +424 h ' ."MFBPCR"\ATV.dial :ASV F*= n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F*g% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*\\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *!' +424 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*e% h % ."MFBPCR"\AS.seek 0.71ml F*K' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*U% h % H ."MFBPCR"\AS.seek 0.735ml F*u' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*D% h % Ű ."MFBPCR"\AS.seek 0.72ml F*n' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*h n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F* % h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*j n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*w % +43 h % F*?% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F* % h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %  ."MFBPCR"\AS.seek 0.604ml F*v'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*W' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*: % +107 h % F*.% h %V ."MFBPCR"\AS.seek 0.01ml F*p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*@% h % ."MFBPCR"\AS.seek 0.58ml F*^' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*B'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*1% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*;' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*c  +21 h  ."MFBPCR"\AS.seek 0ml F*f'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *5'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*>  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *#' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*~ n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *T'R +202 h 'R ."MFBPCR"\SPE.coast F*"  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*2 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*$  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b */' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*- I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*V  +43 h  F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*0  +65 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *S'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*M n +65 h n F*k% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *R' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*_  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *P'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*d n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *z' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*< I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*i  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*8 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *]'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*s n +150 h n ."MFBPCR"\AS.seek 0ml F*J'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*Q  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*' % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*F'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F* 'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*= I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *g'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\\'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *G' +9987 h ' ."MFBPCR"\ATV.dial AIV F*b I +43 h I ."MFBPCR"\AS.seek 0.02ml F*!' +9880 h ' ."MFBPCR"\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.seek 0ml F*e'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*C% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*U  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +21 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*T % +108 h % ."MFBPCR"\AS.seek 0ml F*2'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast .\CS.coast .\PC.coast .\PS.coast C'6 P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 &6 P%5 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537156815.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )$ h  C' ' h  P% %Y h  F* *- h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*/  h  .t\SPE.configure SPEconfig F*\ 2 *A% h %V .t\AS.configure ASconfig S)V% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'6& S)#.0X 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:16:04CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +150 h 1 M&=& h &_ M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &`(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &\\'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +265 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'!& h &W .tHand.open M&e1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'a& h &W C'9% h %4 .t\CSV.dial CSR C'u  +151 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'j'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'w'4 +2010 h '4 #t\Purging external Intake C'?& h &W C'% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'N  h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'r'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'y% h %4 .t\CSV.dial :flush C'v  +76 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'L% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C': +113 h .t\CTV.dial :air .\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'@'4 +506 h '4 C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'p'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'^% h %0 .t\CS.seek 5ml C'1'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'c +75 h .t\CS.seek 0ml C';'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +6 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'"( +416 h ( M&~& h &ӷ M&T% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &q(- +265 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &l'η +91 h 'η .tHand.open M&I1 +150 h 1 M&4% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +90 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:16:09CEST30-Aug-18\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:16:04CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*H% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&$% F* h %\  A(% h %X A(Y. h ., A(-& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (/& h &  M&A& h &- M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &6( +7 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*0 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +159 h ( M&t& h & A(M& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &k' +90 h ' A(S& h &  .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(R& h &  M&{& h & .t\Elbow.seek 2 M&_( +6 h ( M&%& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&|'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &d/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&P% h %; .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.seek CC M&s(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &(' +91 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&\ 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)'+ +6 h + S)J% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'4 +7 h '4 ."CSpush"\CS.coast C'5 +71 h ' .t\SSV.dial :spit,avoiding: :exhaust S)5 +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'y9 h 9Td +100 C'9 h 9kd +100 C'v9 h 9d +100 C'r9 h 9d +100 C'L9 h 9d +100 C' 9 h 9d +100 C':9 h 9d +100 C'9 h 9d +100 C'.9 h 9 d +100 C'w9 h 9 d +100 C'9 h 96d +100 C'9 h 9Ld +100 C'}9 h 9bd +100 C'9 h 9xd +100 C'W9 h 9d +100 C'@9 h 9d +100 C'o9 h 9d +100 C'^9 h 9d +100 C'9 h 9d +100 C'p9 h 9d +100 C'B9 h 9d +100 C'c9 h 9(d +100 C'f9 h 9>d +100 C'19 h 9Td +100 C'59 h 9id +100 C'9 h 9d +100 C'>9 h 9d +100 C'9 h 9d +100 C'#9 h 9d +100 C'K9 h 9d +100 C'C9 h 9d +100 C'T9 h 9  d +100 C')9 h 9 d +100 C'O9 h 9 *d +100 C'9 h 9 ?d +100 C'[9 h 9 Td +100 C'I9 h 9 j d +100 C'~9 h 9 $d +100 C'79 h 9 (d +100 C'9 h 9 ,d +100 C'H9 h 9 0d +100 C'$9 h 9 4d +100 C'9 h 9 8d +100 C'Y9 h 9 1 +150 h 1 M&% h %Y A(#& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&K'υ +21 h 'υ M&C& h & A(T& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &)' +22 h ' A(O% h % ."downElevator"\SE.seek :down A('X +100 ([% h % M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +89 h '\  +13 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'7 @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'H ` +19 h ` .t\AIV.dial :bypass C'$ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&Y1 +2 h 1 M&-& h & M&/% h %\  .t\Elbow.seek FlushPuck::Garage M&4(- +166 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&0'η +91 h 'η .tHand.close M&/ +150 h / M&M& h &- M&l% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'2(W +416 h (W C'R& h &W .tHand.open M&1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%P% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%k  +38 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%|  C'  h +75 .t\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%i  h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%\  +38 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :mixloop P%J +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%Q' +2609 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.seek 0ml P%'' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&8& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +6 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'<( +416 h ( M& & h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +90 h 'η .tHand.open M&F1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'e& h & M&U% h %\  A(a% h %X A(E. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (h( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&n& h &- M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +166 h ( M&j& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &s' +89 h ' A(&& h &  .t\SE.seek 13.71 Pucks below Top Plate A(D'n +125 h 'n A(N& h &  M& & h & .t\Elbow.seek 2 M&y( +7 h ( M&& h & A(r& h &  .t\Forearm.seek 2 + 300 counts M&L'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(.'X M&?& h & M&u% h %; .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.seek CC M&X(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +109 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +90 h ' .t\CC.seek :closed C';(W +416 h (W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'w  +151 h  .t\CSV.dial CTV C',  +19 h  .t\CS.seek 5ml C'o'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'^' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%p  +38 h  .t\PS.seek 2.5ml P%f' +2610 h ' .t\PSV.dial :mixloop P%1 +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%>  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'# @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'C  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'T @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'O  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'B ` +19 h ` .t\CSV.dial :air C'c @ +38 h @ .t\CS.seek 10ml C'[' +2647 h ' .t\CSV.dial CTV C'I  +38 h  .t\CS.seek 0ml C'7'4 +2647 h '4 .t\CBV.dial :waste1 C'H @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'$  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&Y% h %\  A(-% h %X A(/. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (5( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (K& h & M&:& h &ӷ M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &V( +142 h ( M&x& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(!& h & .t\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' M&& h & A(M& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &l'Y +25 h 'Y .t\Elbow.hold M&~, +6 h , .t\Elbow.reconfigure TightElbow A(0- M&$&.,(\ #b h -+ .t\SE.seek 6.04 Pucks below Top Plate A(q' +27 h ' M&{% h %Y A(4& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&S'υ +21 h 'υ M&@& h & A(R& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(A' +36 h ' M&& h & M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +89 h '\  .t\Elbow.seek CC M&k(ӷ +143 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'( +416 h ( A(_& h & A(i& h & M&z& h &ӷ M&\ % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &J( +142 h ( M&"& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&=, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Q1 +150 h 1 M&8% h %Y A(g& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&+'υ +20 h 'υ M&& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +23 h ' A(2% h % ."downElevator"\SE.seek :down A( 'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%`  + A(6 +111 h  P%(% h % .t\PS.seek 0.499ml P%F' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%e  +38 h .t\CBV.dial AIV C'U  +38 h .t\PRV.dial between CBV and air P%a  +19 h  .t\PRV.dial :air P%E  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %n' +251 h ' .t\PRV.dial :waste2 P%9 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %b' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'j& h & .tHand.open M&1 +2 h 1 M&&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'L(W +416 h (W C'D& h &W .tHand.open M&y1 +150 h 1 M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  C'<& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%w  +76 h  .tdelay 2 seconds +200 P%,% h % .t\PS.seek 1.499ml P%v' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%h' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%o' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%^ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%p  +38 h  .tdelay 2 seconds +200 P%'% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %1'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%>' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%f  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%#'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%C  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%T  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %O' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%B +75 h .t\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%[' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%H +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%$' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%- +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%/  +75 h P%K% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %:' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C') & +50 h & .t\AWV.select :waste C' +6 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %m'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'! { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %X' +1312 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%M' +2610 h ' .t\PSV.dial :PML P%W +37 h .t\PS.seek 0ml P%0' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '{& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'4& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*@ @@T .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*R  P% +21 h  ."MFBPCR"delay 20 seconds +91 h P%P% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*V I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *l'6 +66 .t\PSV.dial :waste1 P%6  +38 h  .t\PS.seek 0ml P%*' +1597 h ' .t\PSV.dial PSR P%S +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%q' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%~' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*|  +22 h ' .t\PSV.dial PSR P%% +21 h  F*;% h %6 ."MFBPCR"\AS.seek 0.329ml F*_'ͯ +92 h P%i% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%z' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*\ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%]' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*k  +21 h  F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *8' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%g +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*t I +43 h I F*2% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%+' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%`' +739 h ' ."MFBPCR"delay 5 seconds +500 F*(9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*d n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F* % h % ."MFBPCR"\AS.seek 0.041ml F*5'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%e' +2609 h ' .t\PSV.dial PSR P%U +113 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%E' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%F' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%9 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%n' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %&'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb % ' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *='V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%? +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %G' +5106 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %s' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *u'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*D% h %Q ."MFBPCR"\AS.seek 0.01ml F*y' +171 h ' .t\PSV.dial :mixloop P%. +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %<' +619 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek :empty F*}'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*w% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *,' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*b  +43 h F*v% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'h  +37 h  C'^% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C''' +1625 h ' ."connect"\CSV.dial CTV C'o  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'1'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*f  +22 h ."MFBPCR"delay 3 seconds +300 F*>% h %_ ."MFBPCR"\AS.seek 0.434ml F*C'ݏ +110 h '4 .t\CSV.dial CSR C'T  +37 h  C'#% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'B'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*c  +22 h F*O% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*I'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*H% h %& ."MFBPCR"\AS.seek 0.092ml F*['&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'$  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'-'4 +2647 h '4 C'/& h &W #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&:' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'm( +416 h ( M&& h &ӷ M&X% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +264 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&1 +150 h 1 M&P% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'V& h & M&% h %\  A(6% h %X A(\\. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (S& h &  M&*& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &q( +166 h ( M&|& h & A(;& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(i& h &  ."WCR"\SE.seek 12.71 Pucks below Top Plate A(l' +137 h ' A(~& h &  M&\ & h & ."WCR"\Elbow.seek 2 M&_( +6 h ( M&& h & A(k& h &  ."WCR"\Forearm.seek 2 + 300 counts M&Q'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &z/ +119 h '&0 ."MFBPCR"delay 3 seconds +31 h / ."downElevator"\SE.seek :down M&"& h & A(g'X M&t% h %; ."WCR"\Forearm.seek :retract M&2'\ +91 h '\  ."WCR"\Elbow.seek CC M&](ӷ +46 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&8& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +35 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +13 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&A1 +130 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +20 h 1 M&(% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b * ' +89 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)% h % C'`& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*T  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *#% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *1% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *c' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*O% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *C' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*H'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*I n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*$ % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b */'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*K I +22 h I F*)% h %v ."MFBPCR"\AS.seek 0.059ml F*x'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*! % +21 h % F* % h %H ."MFBPCR"\AS.seek 0.069ml F*3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F*X' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h % q ."MFBPCR"\AS.seek 0.794ml F*4' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*P'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*W I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *V' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*6'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*\\ % +43 h % F*S% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.579ml F*|' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %  ."MFBPCR"\AS.seek 0.604ml F*M'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*\ % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*k n +86 h n ."MFBPCR"\ASV.dial :wasteR F*0  +43 h  ."MFBPCR"\AS.seek 0ml F*z'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*q % +108 h % F*%% h %V ."MFBPCR"\AS.seek 0.59ml F*8' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +108 h n ."MFBPCR"\ASV.dial :wasteR F*l% h %  ."MFBPCR"\AS.seek 0.09ml F*Q' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*_ I +86 h I F*g% h % ."MFBPCR"\AS.seek 0.12ml F*2' +625 h ' ."MFBPCR"\ATV.dial :air3 F*[ % +21 h % F*m% h % ."MFBPCR"\AS.seek 0.13ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*+% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*a n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *E'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*5 I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*j% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*?'\  +1032 h '\  F*&% h %\  ."MFBPCR"\AS.seek 0.5ml F*G'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *N' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*= % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*r  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1709h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*D  +64 h  ."MFBPCR"\ARV.dial :carrier F*.  +11 h  F*n% h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*y n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +21 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *u'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*,  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*h' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*p  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +107 h  ."MFBPCR"delay 7 seconds +700 F*T% h %< ."MFBPCR"\AS.seek 0.594ml F*#'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 F*I  +65 h ."MFBPCR"\ARV.dial :air1 F*H  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*$% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*K % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254601 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C')  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'/ @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'L  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'3 ` +19 h ` ."WCR"\CS.seek 7.288ml C':' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'{  'X  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Y'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '4' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'P  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'\\' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'V @ +76 h @ +50 S)6% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'R% h % ."WCR"\CSV.dial between air and CTV C'* ` +56 h ` ."WCR"\CS.seek 7.288ml C';' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'|  'i  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'M' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'0  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'z' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'q @ +75 h @ +50 S)%% h % ."WCR"\CBV.dial between CSV and waste2b C't  +94 h  C'J% h % ."WCR"\CSV.dial between air and CTV C'l ` +56 h ` ."WCR"\CS.seek 7.288ml C'8' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'_  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'g'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'Q  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '[' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'm  +151 h C'2& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&(% h %\  A(+% h %X A(A. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (`( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&& h & A(a& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &U' +89 h ' A(& h & ."WCR"\SE.seek 6.71 Pucks below Top Plate A(' +196 h ' M&e& h & A(E& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &5'Y +25 h 'Y ."WCR"\Elbow.hold M&F, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(' +27 h ' M&9% h %Y A( & h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&j'υ +21 h 'υ M&& h & A( & h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A(&'w +36 h 'w M&?& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +90 h '\  ."WCR"\Elbow.seek CC M&=(ӷ +142 h (ӷ M&N& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ ."WCR"\Forearm.seek CC M&D' +91 h ' ."WCR"Hand.close M&./ +150 h / ."WCR"\CC.seek :open C'n( +416 h ( A(& h & A(s& h & M&y& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&b& h & A(v& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &w'Y +93 h 'Y ."WCR"\Elbow.hold M&,, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &^1 +150 h 1 M&u% h %Y A(p& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&o'υ +21 h 'υ M&h& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' A(7% h %w ."downElevator"\SE.seek :down A(>'X +100 (T% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +81 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M +2 h 1 M&O& h & M&c% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.seek :garage M&H'η +91 h 'η .tHand.close M&$/ +150 h / M&& h &- M&B% h %η .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +264 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'L& h &W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'X& h &W C'{% h % .t\CSV.dial CSR C':  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'Y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'P'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '4'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'\\& h &W C'V% h %4 .t\CSV.dial :flush C'6  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +37 h @ C'*% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  '|  +38 h  +113  .t\CBV.dial :waste1 C'; @ +75 h @ .t\CS.seek 0ml C'\ '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'~& h &W C'k% h %4 .t\CSV.dial :flush C'0  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +37 h @ C'z% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CTV C'%  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'J'4 +766 h '4 #t\Flushing upper manifold C'l& h &W C'"% h %4 .t\CSV.dial :flush C'_  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'Q% h %p .t\CS.seek 7ml C'g'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'm '2  +38 h +75 .t\CTV.dial :air C'(  +151 h .t\CS.seek 0ml C'+'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'a  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'E'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'd'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'9 +76 h .t\CS.seek 0ml C' '4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C' % h %4 .t\CSV.dial CSR C'?  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'5  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'N' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'r  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'G  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C's'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'y  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  +38 h  .t\CBV.dial :waste2b C'=  +75 h  .t\CS.seek 0ml C'^'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'u  +38 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'h'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 '  +38 h +75 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'T'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'1 +37 h .t\CS.seek 0ml C'#'4 +507 h '4 #t\Flushing upper manifold C'O& h &W C'c% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.seek 5ml C'o'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'C% h %p .t\CS.seek 7ml C'I'i +416 h 'i ."connect"\CSV.dial CBV C'' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'>'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'`'0 +507 h '0 .t\CSV.dial :waste C', +37 h .t\CS.seek 0ml C'$'4 +507 h '4 C'& h &W C'B% h %4 .t\CSV.dial :flush C'f  +38 h  .t\CS.seek 3ml C'H'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'-% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'3& h &W C'X% h %4 .t\CSV.dial :flush C'{  +37 h  .t\CS.seek 5ml C':'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C' % h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  'Y  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'P'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\\& h &W C'V% h %4 .t\CSV.dial :flush C'6  +76 h  .t\CS.seek 5ml C'4'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'*% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  +38 h  .t\CBV.dial :waste2b C'|  +75 h  .t\CS.seek 0ml C';'4 +766 h '4 #tDisinfecting external Intake with kill C'S& h &W C'~% h %4 .t\CSV.dial CSR C'k  +38 h  C'0% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'\ 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'M' +416 h ' ."connect"\CSV.dial CBV C'z .t\CBV.dial CSV C'  +37 h +76 .t\CTV.dial :intake C'%  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C't' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'q  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +606 h '4 .t\CSV.dial CSR C'"  +113 h  C'_% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'J'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'8' +1372 h ' .t\CSV.dial CBV C'Q +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&2& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'A( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&E1 +150 h 1 M&d% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &?(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&U'η +91 h 'η .tHand.close M&/ +150 h / M&5& h &- M&% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &j' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'& h &W .tHand.open M&G1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'y& h &W C' % h %4 .t\CSV.dial CSR C's  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '=% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'v'[ +462 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Purging external Intake C'^& h &W C'h% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'T'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'1  +75 h #t\Flushing upper manifold C'& h &W C'O% h %4 .t\CSV.dial :flush C'c  +76 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +37 h @ C'&% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'o +113 h .t\CTV.dial :air .\CS.seek 0ml C'''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +507 h '0 .t\CSV.dial :waste C'> +38 h .t\CS.seek 0ml C','4 +506 h '4 C'`& h &W C'% h %4 .t\CSV.dial :flush C'B  +38 h  .t\CS.seek 3ml C'f'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'/% h %0 .t\CS.seek 5ml C'-'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'H'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'K +75 h .t\CS.seek 0ml C'L'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&n& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +6 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&:& h &ӷ M&W% h % .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &V'η +91 h 'η .tHand.open M&61 +150 h 1 M&P% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  #t\Completed [mbaPhase] begun 06:00:30 #\Preparing to sleep until Wed Sep 19 06:00:30 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST19-Sep-18 #\Continuing at 06:00CEST19-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:17:35CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST19-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1709h25ml.pcr F**% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*i  +22 h ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*g  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*)% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*E% h %m ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*y  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*b  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*j% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*=  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*u' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*v  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*h'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*c' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*T  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*#  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*w'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1711h25ml.pcr F*'% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*I  +22 h ."MFBPCR"\ATV.dial :air3 F*` % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*$% h %Z ."MFBPCR"\AS.seek 0.81ml F*/'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*H'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +31 h  ."MFBPCR"\ATV.dial :air3 F*3 % +87 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*@'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*M  +42 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *2'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*)  +22 h ."MFBPCR"delay 2 seconds +200 F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*] n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*m' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*E  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*5' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1713h25ml.pcr F*?% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*r  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*='n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*o% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*>  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +11 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F*.'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\\  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*3  +21 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *['Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*Y n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*P' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F** I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*@' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*| % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*i'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*S n +21 h n ."MFBPCR"delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*q  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*M'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1716h25ml.pcr F*"% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*8  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*+'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*e  +64 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*5% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*v  +10 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*c n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*C' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*I  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*$ n +21 h n ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*H  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*X% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1718h25ml.pcr F*{% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*:  +22 h ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +21 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +99 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*z  +31 h  ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*%% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*M'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +21 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ARV.dial :carrier F*+  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*U I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*9 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*G n +21 h n ."MFBPCR"delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*D' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*u @@T ."MFBPCR"\ASV.dial :SPEdelivery F*h  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *1' +424 h ' ."MFBPCR"\ATV.dial :ASV F*T n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*#  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*c'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*o n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b * 'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*''(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*& % +43 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *`' +424 h ' ."MFBPCR"\ATV.dial :air3 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h % ."MFBPCR"\AS.seek 0.71ml F*$' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h % H ."MFBPCR"\AS.seek 0.735ml F*-' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*f% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*H n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*X% h % zG ."MFBPCR"\AS.seek 0.365ml F*K' +7162 h ' ."MFBPCR"\ATV.dial :waste F*n n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*W% h % ."MFBPCR"\AS.seek 0.579ml F*.' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %  ."MFBPCR"\AS.seek 0.604ml F*\\'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*V% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*['V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*@ % +107 h % F*P% h %V ."MFBPCR"\AS.seek 0.01ml F*~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*i% h % ."MFBPCR"\AS.seek 0.58ml F*S' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h % q ."MFBPCR"\AS.seek 0.605ml F*z'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*%  +22 h  ."MFBPCR"\AS.seek 0ml F*q'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *"'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*_  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *8' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Q n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *M'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*J n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +202 h 'R ."MFBPCR"\SPE.coast F*)  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *g' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*E  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *+': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*d  +65 h  F*e% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*a% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *5': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*9% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *?'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*r% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*G n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *y'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*s'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*b  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*j % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*D% h % ."MFBPCR"\AS.seek 0.96ml F*v'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*7'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*T I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +42 h  F*&% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *I' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *'' +9987 h ' ."MFBPCR"\ATV.dial AIV F*C I +43 h I ."MFBPCR"\AS.seek 0.02ml F*`' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*w'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*>% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +43 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*W  +65 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*P% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*~'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +21 h F*k% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' @CEST1537221616.45 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*) n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *M'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*2 % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537329630.89 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' '! h  P% %( h  F* *] h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *g  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)E% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'm& S)#.0X 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:32CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +150 h 1 M&T& h &_ M&7% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &#(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &o'η +91 h 'η .tHand.close M&c/ +150 h / M& & h &- M&&% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +264 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'>(W +416 h (W C'`& h &W .tHand.open M&w1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'/& h &W C'B% h %4 .t\CSV.dial CSR C'-  +150 h  C'H% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'f'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'X +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '{'4 +2009 h '4 #t\Purging external Intake C'& h &W C'K% h %4 .t\CSV.dial :flush C'F  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C': 'N  h +75 .t\CTV.dial :intake C'3  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'6'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'.  +75 h #t\Flushing upper manifold C'Y& h &W C'V% h %4 .t\CSV.dial :flush C'\\  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'[% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'@ +113 h .t\CTV.dial :air .\CS.seek 0ml C'P'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'|'0 +507 h '0 .t\CSV.dial :waste C'4 +37 h .t\CS.seek 0ml C'i'4 +507 h '4 C'& h &W C';% h %4 .t\CSV.dial :flush C'k  +37 h  .t\CS.seek 3ml C'~'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'S% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'% +76 h .t\CS.seek 0ml C'0'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&Q' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C')( +416 h ( M&J& h &ӷ M&2% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &R(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &<'η +91 h 'η .tHand.open M&1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:18:37CEST30-Aug-18\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:32CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*}% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'u& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(!% h %X A((. h ., A(]& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (g& h &  M&& h &- M&E% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &m( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*d % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *e' +160 h ( M&+& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &a' +89 h ' A(& h &  .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ A(& h &  M& & h & .t\Elbow.seek 2 M&( +6 h ( M&U& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&9& A(r'X h & M&?% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +54 h 'X ."downElevator"\SE.coast A(5 +88 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +91 h ' .t\CC.seek :closed C'p(W +416 h (W .tHand.open M&h1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)j+ +6 h + S)s% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'V9 h 9Td +100 C'39 h 9kd +100 C'\\9 h 9d +100 C'69 h 9d +100 C'[9 h 9d +100 C'9 h 9d +100 C'@9 h 9d +100 C'*9 h 9d +100 C'P9 h 9 d +100 C'{9 h 9 d +100 C'Y9 h 96d +100 C'|9 h 9Ld +100 C'9 h 9bd +100 C';9 h 9xd +100 C'9 h 9d +100 C'i9 h 9d +100 C'\ 9 h 9d +100 C'S9 h 9d +100 C'k9 h 9d +100 C'~9 h 9d +100 C'z9 h 9d +100 C'%9 h 9(d +100 C'q9 h 9>d +100 C'9 h 9Td +100 C'"9 h 9id +100 C'49 h 9d +100 C'_9 h 9d +100 C't9 h 9d +100 C'89 h 9d +100 C'$9 h 9d +100 C'>9 h 9d +100 C'29 h 9  d +100 C'9 h 9 d +100 C'I9 h 9 *d +100 C'Q9 h 9 ?d +100 C'Z9 h 9 Td +100 C'9 h 9 j d +100 C'J9 h 9 $d +100 C'l9 h 9 (d +100 C'M9 h 9 ,d +100 C'}9 h 9 0d +100 C'9 h 9 4d +100 C'!9 h 9 8d +100 C'(9 h 9 & h & A(2& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(I% h %f ."downElevator"\SE.seek :down A(Q'X +100 (Z% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +72 h 'X ."downElevator"\SE.coast A(5 +18 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'l @ +18 h @ .t\CS.seek 10ml C'M' +7 h ' .t\CSV.dial between air and CTV C'} ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'!& h & .tHand.open M&(1 +2 h 1 M&]& h & M&g% h %\  .t\Elbow.seek FlushPuck::Garage M&L(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&d'η +91 h 'η .tHand.close M&u/ +150 h / M& & h &- M&<% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &U' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%9% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%a  +38 h  .t\PS.seek 0ml P%?' +1597 h ' .t\PSV.dial PSR P%^ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%r' +2609 h ' .t\PSV.dial :air P%h  +38 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%s +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%5  +38 h  .t\PS.seek 2.5ml P%b' +2609 h ' .t\PSV.dial :mixloop P%T +38 h .t\PS.seek 0ml P%j' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( M&e& h &ӷ M&D% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +264 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'w& h & M&,% h %\  A(/% h %X A(O. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (H( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &  M&& h &- M&B% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &X( +166 h ( M&n& h & A(K& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(f& h &  .t\SE.seek 10.71 Pucks below Top Plate A(N' +155 h ' A(& h &  M&W& h & .t\Elbow.seek 2 M&V( +7 h ( M&3& h & A(6& h &  .t\Forearm.seek 2 + 300 counts M&['; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down A(P'X M&& h & M&-% h %; .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.seek CC M&:(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +78 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'0(W +416 h (W .tHand.open M&1 +150 h 1 M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'{  +151 h  .t\CSV.dial CTV C'p  +18 h  .t\CS.seek 5ml C'\ 'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'S' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'k'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%q' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%;  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Y +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%_  +151 h  .t\PS.seek :empty P%t' +2609 h ' .t\CBV.dial :waste1 C'8 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'>  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'I  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'z ` +19 h ` .t\CSV.dial :air C'% @ +38 h @ .t\CS.seek 10ml C'Z' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'l'4 +2648 h '4 .t\CBV.dial :waste1 C'} @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&(% h %\  A(]% h %X A(g. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ("( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ($& h & M&@& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &E( +143 h ( M&x& h & A(Q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(`& h & .t\SE.seek 8.71 Pucks below Top Plate A(4' +175 h ' M&u& h & A( & h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &<'Y +26 h 'Y .t\Elbow.hold M&J, +6 h , .t\Elbow.reconfigure TightElbow A(d- M&$&.,(\ #b h -+ .t\SE.seek 9.04 Pucks below Top Plate A(R'f +27 h 'f M& % h %Y A(L& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +20 h 'υ M&i& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' .t\SE.seek 10.04 Pucks below Top Plate A('U +36 h 'U M&& h & M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  .t\Elbow.seek CC M&a(ӷ +143 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(& h & M&& h &ӷ M&h% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &s( +142 h ( M&)& h & A(5& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&T, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &b1 +150 h 1 M&G% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &1'υ +20 h 'υ M& & h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' A( % h %U ."downElevator"\SE.seek :down A(e'X +100 (D% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +62 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast .\PSV.dial :air A(6 P%#  +113 h  P%=% h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%7 +38 h .t\PRV.dial :air P%c  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%w  +37 h .t\CBV.dial AIV C',  +38 h .t\PRV.dial between CBV and air P%/  +19 h  .t\PRV.dial :air P%O  +19 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%B @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&K1 +2 h 1 M&f& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&W& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&6'η +91 h 'η .tHand.close M&/ +150 h / M&*& h &- M&% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'N& h &W .tHand.open M&V1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%.  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%{  +75 h  .tdelay 2 seconds +200 P%p% h % .t\PS.seek 1.499ml P%\\' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%H' +1094 h ' .t\PSV.dial :air P%M  +38 h  .t\PS.seek 0ml P%\ ' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%S +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%~  +38 h  .tdelay 2 seconds +200 P%j% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%;  +75 h .tdelay 2 seconds +200 P%Y% h %r .t\PS.seek 0.475ml P%_' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%q  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%8'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%>  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%2  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %I' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%z +75 h .t\PTV.dial :PRV,avoiding: :puck P%% @ +76 h @ .t\PS.seek 0ml P%k' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%Z' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%} +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +76 h  .t\PS.seek 2.5ml P%(' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%] +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%g  +75 h P%$% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %@' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %Q'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'` { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%u  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :PML P%d +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 ' & h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'L& F*i @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%9% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*E I +128 h I F*4% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *<'6 +66 .t\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%R' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%U +21 h  F*0% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*h % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%?' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*a  +22 h  F*b% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *G' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F* % h % ."MFBPCR"\AS.seek 0.469ml F*r' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%s +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%#' +740 h ' ."MFBPCR"delay 5 seconds +500 F*=9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*y n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*e% h % ."MFBPCR"\AS.seek 0.041ml F*"'% +336 h ' .t\PSV.dial PSR P%t +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%c +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%w' +2610 h ' .t\PSV.dial PSR P%, +113 h P%/% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%B +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%K +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %f'k +2678 h 'k .t\PSV.dial :waste1 P%&  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %W' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *T'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%*' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %'' +5107 h ' .t\PSV.dial :air P%6  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*|  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.05ml F*-'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*N% h %Q ."MFBPCR"\AS.seek 0.01ml F*V' +171 h ' .t\PSV.dial :mixloop P%P +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*.  +85 h  F*{% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *p' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*\\% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *M'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'H  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'j' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\  ';  +37 h  +113  .t\CBV.dial :waste1 C'Y @ +38 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*q  +21 h ."MFBPCR"delay 3 seconds +300 F*_% h %_ ."MFBPCR"\AS.seek 0.434ml F*>'ݏ +110 h '4 .t\CSV.dial CSR C'2  +38 h  C'8% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'z'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*%  +21 h F*I% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*k n +108 h n F*}% h %& ."MFBPCR"\AS.seek 0.092ml F*Z'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +1626 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C']'4 +2648 h '4 C'g& h &W #tGaraging FlushPuck in Collection Clamp M&$& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'Q( +416 h ( M&u& h &ӷ M&:% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&9% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'E& h & M&4% h %\  A(m% h %X A(o. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&^& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &R( +165 h ( M&& h & A(0& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +90 h ' A(& h &  ."WCR"\SE.seek 9.71 Pucks below Top Plate A(<' +166 h ' A(J& h &  M&h& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&5& h & A(a& h &  ."WCR"\Forearm.seek 2 + 300 counts M&b'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +88 h '&0 ."MFBPCR"delay 3 seconds +62 h / ."downElevator"\SE.seek :down A()'X M&& h & M&+% h %; ."WCR"\Forearm.seek :retract M& '\ +90 h '\  ."WCR"\Elbow.seek CC M&?(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&G& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +5 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +43 h ' ."WCR"\CC.seek :closed C'D(W +416 h (W ."WCR"Hand.open M&1 +101 h 'R ."MFBPCR"\ATV.dial :air2 F*1  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *=' +28 h 1 M&y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +6 h + S)t% h % C'#& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'c  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*k% h % ."MFBPCR"\AS.seek 0.279ml F*}'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*!% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *g'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*$ I +21 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F*x'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*` % +21 h % F*X% h %H ."MFBPCR"\AS.seek 0.069ml F*F' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +21 h F*u% h % ."MFBPCR"\AS.seek 0.769ml F*:' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % q ."MFBPCR"\AS.seek 0.794ml F*L' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*9'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*d I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *E' +4416 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek :empty F*m'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*o % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*h% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*5' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*a n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*R % +107 h % F*U% h %V ."MFBPCR"\AS.seek 0.59ml F*G' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*s n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*<' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*b  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*)% h % ."MFBPCR"\AS.seek 0.12ml F* ' +625 h ' ."MFBPCR"\ATV.dial :air3 F*Z % +21 h % F*Q% h % ."MFBPCR"\AS.seek 0.13ml F*1' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*y% h % ."MFBPCR"\AS.seek 0.23ml F*t' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*, n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*/  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *O'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*7 @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*" I +86 h I ."MFBPCR"\AS.seek 0ml F*K'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*='(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  #"MFBPCR"\Swish #1 of 2 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1031 h '\  F*n% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*W% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*f% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*|% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*T % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b **'V +3472 h 'V ."MFBPCR"\SPE.coast F*6  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1909h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*N  +64 h  ."MFBPCR"\ARV.dial :carrier F*P  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*p  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*S% h %Fj ."MFBPCR"\AS.seek 0.524ml F*H' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*~  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*l' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Y % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.536ml F*;'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +107 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*}  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*!'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*$ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254631 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'g @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '`' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'X @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'[  +94 h  C'u% h % ."WCR"\CSV.dial between air and CTV C'F ` +19 h ` ."WCR"\CS.seek 7.288ml C'@' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '('4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'd  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'9  +38 h  C'4% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'o' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'E @ +76 h @ +50 S)m% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'^ ` +56 h ` ."WCR"\CS.seek 7.288ml C'0' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'J'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'h  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'a  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'5% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'R @ +75 h @ +50 S)U% h % ."WCR"\CBV.dial between CSV and waste2b C'+  +94 h  C's% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'G' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'b  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ')'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'<  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Z' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'Q  +151 h C' & h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(y% h %X A(1. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (#( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (c& h & M&t& h &ӷ M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&& h & A(,& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +89 h ' A(r& h & ."WCR"\SE.seek 9.71 Pucks below Top Plate A(A' +166 h ' M&w& h & A(O& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &"'Y +25 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(K- M&$&.,(\ #b h -+ ."WCR"\SE.seek 10.04 Pucks below Top Plate A(&'U +27 h 'U M&B% h %Y A(=& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &n'υ +21 h 'υ M&3& h & A(W& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 11.04 Pucks below Top Plate A(f'D +37 h 'D M&& h & M&|% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +89 h '\  ."WCR"\Elbow.seek CC M&T(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ ."WCR"\Forearm.seek CC M&N' +91 h ' ."WCR"Hand.close M&P/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(*& h & A( & h & M&V& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&.'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&C& h & A(\\& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &{'Y +92 h 'Y ."WCR"\Elbow.hold M&p, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &S1 +150 h 1 M&-% h %Y A(~& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&\ 'υ +21 h 'υ M&H& h & A(Y& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &j' +22 h ' A(l% h %D ."downElevator"\SE.seek :down A(_'X +100 (2% h % M&;% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +52 h 'X ."downElevator"\SE.coast A(5 +38 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&81 +2 h 1 M&I& h & M&%% h %\  .t\Elbow.seek FlushPuck::Garage M&M(- +165 h (- M&>& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&}'η +91 h 'η .tHand.close M&/ +150 h / M&k& h &- M&z% h %η .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'[& h &W .tHand.open M&u1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C':% h % .t\CSV.dial CSR C'@  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'('p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'd% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '9'[ +463 h '[ .t\CSV.dial :waste C'4 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'o& h &W C'E% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'^% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'0 @ +76 h @ .t\CS.seek 0ml C'h'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'J& h &W C'a% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'5'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  +37 h  .t\CBV.dial :waste2b C'+  +76 h  .t\CS.seek 0ml C's'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'b  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'<% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q '  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'y'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'1'0 +507 h '0 .t\CSV.dial :waste C'c +37 h .t\CS.seek 0ml C't'4 +507 h '4 C' & h &W C'% h %4 .t\CSV.dial :flush C',  +38 h  .t\CS.seek 3ml C'r'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +38 h @ C'w% h %0 .t\CS.seek 5ml C'O'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'e'0 +507 h '0 .t\CSV.dial :waste C'B +75 h .t\CS.seek 0ml C'='4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'W% h %4 .t\CSV.dial CSR C'  +150 h  C'|% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'/'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'A .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'"  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'6  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'&  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  'D  +37 h  +113  .t\CBV.dial :waste1 C'* @ +38 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'V  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'C'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +710 h ' ."connect"\CSV.dial CTV C'N  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'T  +76 h  .t\CS.seek 0ml C'S'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'-  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'H'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l '.  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'2'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C';'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'8'4 +506 h '4 #t\Flushing upper manifold C'I& h &W C'%% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'>% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'j +113 h .t\CTV.dial :air .\CS.seek 0ml C'_'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'#'0 +506 h '0 .t\CSV.dial :waste C'p +38 h .t\CS.seek 0ml C''4 +506 h '4 C'k& h &W C'z% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 3ml C'}'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'g @ +37 h @ C']% h %0 .t\CS.seek 5ml C'M'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'[ +75 h .t\CS.seek 0ml C'u'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'F& h &W C' % h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'@'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'X% h %p .t\CS.seek 7ml C'Z'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  '(  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +38 h @ .t\CS.seek 0ml C'9'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'o& h &W C'E% h %4 .t\CSV.dial :flush C'm  +75 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'^% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'0'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'J% h %4 .t\CSV.dial CSR C'a  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'h'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '5  +38 h +75 .t\CTV.dial :intake C'U  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'+' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'R  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C's'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1373 h ' .t\CSV.dial CBV C'< +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&y' +91 h ' .tHand.close M&c/ +150 h / .t\CC.seek :open C'1( +416 h ( M& & h &ӷ M&% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +265 h (- M&?& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &r'η +90 h 'η .tHand.open M&O1 +150 h 1 M&K% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&31 +2 h 1 M&W& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&|& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &- .t\Forearm.seek :garage M&/'η +91 h 'η .tHand.close M&A/ +150 h / M&"& h &- M&% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' .t\CC.seek :closed C'(W +416 h (W C''& h &W .tHand.open M&D1 +150 h 1 M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'V& h &W C'% h %4 .t\CSV.dial CSR C'  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'T% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '\\'[ +463 h '[ .t\CSV.dial :waste C'- +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +2010 h '4 #t\Purging external Intake C'S& h &W C'H% h %4 .t\CSV.dial :flush C'.  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'Y  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'2'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C';& h &W C'I% h %4 .t\CSV.dial :flush C'%  +75 h  .t\CS.seek 5ml C'{'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'f% h %p .t\CS.seek 7ml C'>'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +113 h .t\CTV.dial :air .\CS.seek 0ml C'j'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'_ +38 h .t\CS.seek 0ml C'p'4 +506 h '4 C'#& h &W C'k% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'q'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'g% h %0 .t\CS.seek 5ml C']'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'}'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'$ +75 h .t\CS.seek 0ml C'['4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&u' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'X( +416 h ( M&@& h &ӷ M&d% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +265 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &E'η +91 h 'η .tHand.open M&m1 +150 h 1 M&9% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 06:00:46 #\Preparing to sleep until Fri Sep 21 06:00:46 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST21-Sep-18 #\Continuing at 06:00CEST21-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:20:02CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST21-Sep-18" +235183 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1909h25ml.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*5'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*)  +31 h  ."MFBPCR"\ATV.dial :air3 F*c % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +21 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*,  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1911h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*/  +42 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F**  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*C  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*T  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*-' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\\  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*.  +108 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +21 h n ."MFBPCR"delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*> % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1911h25ml.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*# % +86 h % F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*F % +86 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*L'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1913h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*b  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*c  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*? n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*Q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*O  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.536ml F*,'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +107 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F*='\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*/% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*B % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1913h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*6  +22 h ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*S  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +65 h  F*.% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*#% h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*_  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1916h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*P'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Z'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*^ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*i' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*5  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*R  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1916h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*G  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*'% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*1  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *-'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1918h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*.  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*2  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*% n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*7  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*>' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +22 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*]  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*$' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*M  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*}  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1918h25ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*@  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*m% h %Z ."MFBPCR"\AS.seek 0.81ml F*u'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*5% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1920h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +10 h  F*K% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*W n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*B % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +21 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*T  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*S% h % \\ ."MFBPCR"\AS.seek 0.326ml F*.'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1920h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*- @@T ."MFBPCR"\ASV.dial :SPEdelivery F*H  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Y  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*2 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*8  +43 h  F*;% h % ."MFBPCR"\AS.seek 0.62ml F*%'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*\ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *7'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*j'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *#' +423 h ' ."MFBPCR"\ATV.dial :air3 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*_' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*{% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h % H ."MFBPCR"\AS.seek 0.735ml F*]' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*q% h % Ű ."MFBPCR"\AS.seek 0.72ml F*M' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*} n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F* % h % zG ."MFBPCR"\AS.seek 0.365ml F*$' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*d% h % ."MFBPCR"\AS.seek 0.579ml F*P' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %  ."MFBPCR"\AS.seek 0.604ml F*o'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*E% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*!  +21 h  ."MFBPCR"\AS.seek 0ml F*Z'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*i % +108 h % F*9% h %V ."MFBPCR"\AS.seek 0.01ml F*J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*5% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*+ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AS.seek 0ml F*R'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*b  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *G' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b * '^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*s n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*c n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *)' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*? I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*O  +43 h  F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *y': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*K  +65 h  F*w% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *e'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*W n +65 h n F*,% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *|' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *"': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*r  +65 h  F*B% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*t n +64 h n F*6% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *'' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F**  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*D n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *V'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*n % +42 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +21 h F*N% h % ."MFBPCR"\AS.seek 0.96ml F*\\'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %0 ."MFBPCR"\AS.seek 0.985ml F*.' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*l'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*2 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *;'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\ 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *I'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*7  +43 h  F*f% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *j' +9988 h ' ."MFBPCR"\ATV.dial AIV F*> I +43 h I ."MFBPCR"\AS.seek 0.02ml F*#' +9880 h ' ."MFBPCR"\ATV.dial :waste F*k n +129 h n ."MFBPCR"\AS.seek 0ml F*{'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*_% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*}% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +65 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *F'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*9% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' @CEST1537394431.76 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*c'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast P%6 M&5 + C'5 M&6 P%5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537502446.33 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )  h  C' '` h  P% % h  F* *? h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *)  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)O% h % .t\SS.configure SSconfig C'Q& S)#.0X 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:21:00CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #5) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&.1 +150 h 1 M&2& h &_ M&l% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &8(- +166 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &\ 'η +91 h 'η .tHand.close M&%/ +150 h / M&7& h &- M&f% h %η .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'#& h &W .tHand.open M&{1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'z% h %4 .t\CSV.dial CSR C']  +151 h  C'}% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'q'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'M% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ':'4 +2010 h '4 #t\Purging external Intake C'& h &W C'$% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'd' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'@ .t\CBV.dial CSV C'  h +75 .t\CTV.dial :intake C'F  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'm'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'P  +75 h #t\Flushing upper manifold C'(& h &W C'E% h %4 .t\CSV.dial :flush C'o  +76 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'Z% h %p .t\CS.seek 7ml C'^'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'i +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'L +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'0% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C'J'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'% h %0 .t\CS.seek 5ml C'5'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'U +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'( +416 h ( M&s& h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &v'η +91 h 'η .tHand.open M&1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:21:04CEST30-Aug-18\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:21:00CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*c% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'-& ."readyMFB"\AFV.select :holdingCoil h & F* M& % h %\  A(`% h %X A(. h ., A(?& h &  .t\SC.reconfigure SCloose A($.(\ 1~b ()& h &  M&& h &- M&O% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Q( +7 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*K % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *w' +159 h ( M&y& h & A(W& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +90 h ' A(e& h &  .t\SE.seek 8.71 Pucks below Top Plate A(A' +175 h ' A(|& h &  M&=& h & .t\Elbow.seek 2 M&3( +6 h ( M&/& h & A(r& h &  .t\Forearm.seek 2 + 300 counts M&B'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down M&& A(6'X h & M&% h %; .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.seek CC M&(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &T' +91 h ' .t\CC.seek :closed C'~(W +416 h (W .tHand.open M&H1 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)n+ +6 h + S) % h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  h  ."readyMFB"\ASV.dial :waste1 F*j  h +18305 ."connect"\SSV.dial CBV .t\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'8% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X9 h 9$d +100 C'j9 h 9(d +100 C'89 h 9 ,d +100 C'9 h 9$0d +100 C'Y9 h 9<4d +100 C'#9 h 9S8d +100 C'{9 h 9kd +100 C'E9 h 9Td +100 C'F9 h 9kd +100 C'o9 h 9d +100 C'm9 h 9d +100 C'Z9 h 9d +100 C'!9 h 9d +100 C'i9 h 9d +100 C'^9 h 9d +100 C'99 h 9 d +100 C':9 h 9 d +100 C'(9 h 96d +100 C'9 h 9Ld +100 C'9 h 9bd +100 C'09 h 9xd +100 C'u9 h 9d +100 C'9 h 9d +100 C'h9 h 9d +100 C'9 h 9d +100 C'a9 h 9d +100 C'J9 h 9d +100 C'9 h 9d +100 C'U9 h 9(d +100 C'R9 h 9>d +100 C'59 h 9Td +100 C'9 h 9id +100 C'L9 h 9d +100 C'b9 h 9d +100 C'+9 h 9d +100 C'G9 h 9d +100 C'9 h 9d +100 C'_9 h 9d +100 C' 9 h 9  d +100 C'<9 h 9 d +100 C'9 h 9 *d +100 C'49 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C's9 h 9 $d +100 C'9 h 9 (d +100 C' 9 h 9 ,d +100 C'c9 h 9 0d +100 C' 9 h 9 4d +100 C'`9 h 9 8d +100 C'9 h 9 'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'j  +38 h C'8% h %p .t\CS.seek 7ml C''i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'Y @ +38 h @ .t\CS.seek 10ml C'#' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'{ ` +131 h ` #tLysing Complete C'p& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&g% h %\  A(I% h %X A(}. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (1& h & M&M& h &ӷ M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &]( +142 h ( M& & h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +90 h ' A($& h & .t\SE.seek 10.71 Pucks below Top Plate A(' +155 h ' M&q& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &@'Y +25 h 'Y .t\Elbow.hold M&P, +7 h , .t\Elbow.reconfigure TightElbow A(d- M&$&.,(\ #b h -+ .t\SE.seek 11.04 Pucks below Top Plate A('D +26 h 'D M&E% h %Y A(F& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &o'υ +21 h 'υ M&m& h & A(Z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &!' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(i'3 +36 h '3 M&^& h & M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +89 h '\  .t\Elbow.seek CC M&((ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&0' +91 h ' .tHand.close M&u/ +150 h / .t\CC.seek :open C'( +416 h ( A(h& h & A(& h & M&a& h &ӷ M&J% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &U( +142 h ( M&R& h & A(5& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&L, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &b1 +150 h 1 M&+% h %Y A(G& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&_& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +23 h ' A(% h %3 ."downElevator"\SE.seek :down A(4'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C' ' +6 h ' .t\CSV.dial between air and CTV C'c ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&1 +2 h 1 M&?& h & M&)% h %\  .t\Elbow.seek FlushPuck::Garage M&[(- +166 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&K'η +91 h 'η .tHand.close M&-/ +150 h / M&W& h &- M&v% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' .t\CC.seek :closed C'X(W +416 h (W C'|& h &W .tHand.open M&t1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%r +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Q' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%,  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%S +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%B  C'A  h +75 .t\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%'  h .t\PS.seek 0ml P%6' +2610 h ' .t\PSV.dial :air P%H  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%C' +2610 h ' .t\PSV.dial :mixloop P%2 +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&D& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +7 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'( +416 h ( M&f& h &ӷ M&N% h % .t\Forearm.seek :retract M&%'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +265 h (- M&j& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &>'η +91 h 'η .tHand.open M&Y1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'{& h & M&p% h %\  A(g% h %X A(I. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (}( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (1& h &  M&M& h &- M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +166 h ( M&& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(q& h &  .t\SE.seek 7.71 Pucks below Top Plate A(' +185 h ' A(d& h &  M&&& h & .t\Elbow.seek 2 M&E( +7 h ( M&F& h & A(m& h &  .t\Forearm.seek 2 + 300 counts M&Z'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +150 h / ."downElevator"\SE.seek :down M&9& A('X h & M&]% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&@(ӷ +93 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&!1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C':  +151 h  .t\CSV.dial CTV C'~  +18 h  .t\CS.seek 5ml C'h'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'a'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%J  +38 h  .t\PS.seek 2.5ml P%R' +2609 h ' .t\PSV.dial :mixloop P%5 +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%0  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%( +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%b  +151 h  .t\PS.seek :empty P%+' +2610 h ' .t\CBV.dial :waste1 C'G @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'U @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2648 h '4 .t\CBV.dial :waste1 C'c @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'`& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(?% h %X A(). h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&i& h &ӷ M&<% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &O( +143 h ( M&x& h & A(4& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A(#& h & .t\SE.seek 11.71 Pucks below Top Plate A(L'Ɛ +146 h 'Ɛ M&-& h & A(W& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &v'Y +25 h 'Y .t\Elbow.hold M&s, +7 h , .t\Elbow.reconfigure TightElbow A(K- M&$&.,(\ #b h -+Ɛ .t\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M&=% h %Y A([& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&e'υ +21 h 'υ M&& h & A(|& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' .t\SE.seek 13.04 Pucks below Top Plate A('" +36 h '" M&r& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +90 h '\  .t\Elbow.seek CC M&,(ӷ +142 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&B/ +150 h / .t\CC.seek :open C'A( +416 h ( A(3& h & A('& h & M&*& h &ӷ M&H% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A("& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&2, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&D% h %Y A(;& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'υ +21 h 'υ M&7& h & A(y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(X% h %" ."downElevator"\SE.seek :down A(f'X +100 (N% h % M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +33 h 'X ."downElevator"\SE.coast A(5 +56 h '\  .t\SC.coast .\PSV.dial :air A(6 P%8  +113 h  P%T% h % .t\PS.seek 0.499ml P%Y' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%l +38 h .t\PRV.dial :air P%%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%{  +38 h .t\CBV.dial AIV C'p  +37 h .t\PRV.dial between CBV and air P%g  +19 h  .t\PRV.dial :air P%I  +19 h  P%1% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %M' +250 h ' .t\PRV.dial :waste2 P%z @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %>' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&$1 +2 h 1 M&q& h & M&w% h %\  .t\Elbow.seek FlushPuck::Garage M&d(- +165 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.seek :garage M&m'η +91 h 'η .tHand.close M&/ +150 h / M&^& h &- M&9% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C'& h &W .tHand.open M&E1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%! @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%P  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%:  +75 h  .tdelay 2 seconds +200 P%~% h % .t\PS.seek 1.499ml P%o' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%}' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%h' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%J  +37 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %5'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%0  +75 h .tdelay 2 seconds +200 P%(% h %r .t\PS.seek 0.475ml P%b' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%R  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%G'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%_  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%U @ +75 h @ .t\PS.seek 0ml P%a' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%c +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%`  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%? +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%)  +76 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %i' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'< & +50 h & .t\AWV.select :waste C' +6 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %4'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'# { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %@' +1313 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :PML P%K +37 h .t\PS.seek 0ml P%=' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '[& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'u& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*|  P%r +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%k' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*O I +129 h I F*L% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *v'6 +66 .t\PSV.dial :waste1 P%Q  +37 h  .t\PS.seek 0ml P%S' +1598 h ' .t\PSV.dial PSR P%e +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*B  +22 h ' .t\PSV.dial PSR P%/ +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*3'ͯ +92 h P%'% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%*' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*H % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%"  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*,  +22 h  F*C% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *D' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%; +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +217 ."MFBPCR"\ATV.dial :AIV F*y I +43 h I F*X% h % ."MFBPCR"\AS.seek 0.469ml F*6' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%N' +2610 h ' .t\PSV.dial PSR P%. +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%8' +739 h ' ."MFBPCR"delay 5 seconds +500 F*T9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*V n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%+ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%l' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%{' +2610 h ' .t\PSV.dial PSR P%p +113 h P%g% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%I' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%Y' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%M' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%$ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %q'k +2679 h 'k .t\PSV.dial :waste1 P%w  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %&' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*F I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *2'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%^' +2610 h ' .t\PSV.dial :mixloop P%9 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %j' +5107 h ' .t\PSV.dial :air P%m  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *]'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*A I +42 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*E' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*! n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*P  +86 h  F*:% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *~' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*>  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b * '_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'}  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'J'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'0  'h  +38 h  +113  .t\CBV.dial :waste1 C'( @ +37 h @ .t\CS.seek 0ml C'5'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*R  +22 h ."MFBPCR"delay 3 seconds +300 F*b% h %_ ."MFBPCR"\AS.seek 0.434ml F*_'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'G% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*U  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*a n +107 h n F*c% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'`' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'?'4 +2647 h '4 C')& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'4( +415 h ( M&-& h &ӷ M&@% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &|'η +91 h 'η .tHand.open M&r1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'O& h & M&L% h %\  A(Q% h %X A(\ . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (k( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (e& h &  M&S& h &- M&t% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&B& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &/' +89 h ' A('& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(v' +196 h ' A(s& h &  M&H& h & ."WCR"\Elbow.seek 2 M&3( +6 h ( M&"& h & A(,& h &  ."WCR"\Forearm.seek 2 + 300 counts M&C'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +58 h '&0 ."MFBPCR"delay 3 seconds +92 h / ."downElevator"\SE.seek :down A(;'X M&& h & M&y% h %; ."WCR"\Forearm.seek :retract M&X'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +104 h 'X ."downElevator"\SE.coast A(5 +13 ."MFBPCR"\ATV.dial :waste2 F*D % +25 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +18 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +73 h ' ."WCR"\CC.seek :closed C'N(W +416 h (W ."WCR"Hand.open M&1 +71 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *T' +57 h 1 M&V% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +6 h + S)+% h % C'8& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'%  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +65 h n ."MFBPCR"\ASV.dial :wasteR F*o  +21 h  ."MFBPCR"\AS.seek 0.3ml F*~': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*}'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*J  +86 h F*0% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *h'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*( I +43 h I F*n% h %N ."MFBPCR"\AS.seek 0.109ml F*R'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*b n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *G% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b * ' ?x +3301 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *5% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *U' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *_' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*a% h % ."MFBPCR"\AS.seek 0.279ml F*c'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*`% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *)'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F*<% h %v ."MFBPCR"\AS.seek 0.059ml F*x'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*# % +21 h % F* % h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*-% h % ."MFBPCR"\AS.seek 0.769ml F*@' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +21 h % ."MFBPCR"delay 3 seconds +300 F*[% h % q ."MFBPCR"\AS.seek 0.794ml F*u' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*r% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*K I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *O' +4416 h ' ."MFBPCR"\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.seek :empty F*Q'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*\ % +42 h % F*e% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *S' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*|% h % ."MFBPCR"\AS.seek 0.579ml F*B' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*H% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*"' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*, n +86 h n ."MFBPCR"\ASV.dial :wasteR F*=  +43 h  ."MFBPCR"\AS.seek 0ml F**'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*/% h %V ."MFBPCR"\AS.seek 0.59ml F* ' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*7 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*D% h %  ."MFBPCR"\AS.seek 0.09ml F*v' +10076 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*C  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*3 I +86 h I F*;% h % ."MFBPCR"\AS.seek 0.12ml F*X' +626 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*4% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*V% h % ."MFBPCR"\AS.seek 0.23ml F*+' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*%'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*k% h %X ."MFBPCR"\AS.seek 0.24ml F*6' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*g n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*p  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *I'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*l @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*$'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*T'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*w  +43 h  #"MFBPCR"\Swish #1 of 2 F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *F'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*&% h %(A ."MFBPCR"\AS.seek 0.6ml F*9'\  +1032 h '\  F*q% h %\  ."MFBPCR"\AS.seek 0.5ml F*j'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *d' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*2 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3473 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2109h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*M% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *P'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +22 h ."MFBPCR"delay 2 seconds +200 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*h'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*b  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*G'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*_  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*c  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254660 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'<  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C') @ +37 h @ ."WCR"\CS.reconfigure CSfast C'#d @b ."WCR"\CS.seek 10ml C'#' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'Z  +94 h  C'-% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  '@  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'r  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'L% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'\ ' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'O @ +76 h @ +50 S)Q% h % ."WCR"\CBV.dial between CSV and waste2b C't  +94 h  C'|% h % ."WCR"\CSV.dial between air and CTV C'S ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'B  ''  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 's'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'H  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C',  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'=  +37 h  C'"% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'*' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)/% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'7% h % ."WCR"\CSV.dial between air and CTV C'D ` +57 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'3  'C  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ';'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'v  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'4  +150 h C'X& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(V% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (8( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&+& h &ӷ M&.% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +143 h ( M&%& h & A(g& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &p' +89 h ' A(6& h & ."WCR"\SE.seek 12.71 Pucks below Top Plate A(1' +137 h ' M&{& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &'Y +25 h 'Y ."WCR"\Elbow.hold M&Y, +6 h , ."WCR"\Elbow.reconfigure TightElbow A($- M&$&.,(\ #b h -+ ."WCR"\SE.seek 13.04 Pucks below Top Plate A(w'" +27 h '" M&z% h %Y A(T& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&F& h & A(&& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 14.04 Pucks below Top Plate A(q' +36 h ' M&9& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +89 h '\  ."WCR"\Elbow.seek CC M&2(ӷ +143 h (ӷ M&d& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'M( +416 h ( A(^& h & A(& h & M&E& h &ӷ M&!% h % ."WCR"\Forearm.seek :retract M&P'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +142 h ( M&>& h & A(o& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &:'Y +93 h 'Y ."WCR"\Elbow.hold M&~, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&]% h %Y A(J& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&0'υ +20 h 'υ M&}& h & A((& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +23 h ' A(% h % ."downElevator"\SE.seek :down A(b'X +100 ( % h %v M&h% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&G1 +2 h 1 M&& h & M&U% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M& / +150 h / M&a& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'Z& h &W .tHand.open M&-1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'@& h &W C'[% h % .t\CSV.dial CSR C'i  +38 h  C'r% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'K% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'L +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'u'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'\ & h &W C'O% h %4 .t\CSV.dial :flush C'Q  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +37 h @ C'S% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CTV C'B  .t\CTV.dial CSV C''  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'H'4 +766 h '4 #t\Flushing lower manifold's waste2b port C's& h &W C',% h %4 .t\CSV.dial :flush C'=  +75 h  .t\CS.seek 5ml C'"'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +37 h @ C'*% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'/  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'y  +75 h  .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing upper manifold C'D& h &W C'% h %4 .t\CSV.dial :flush C'C  +75 h  .t\CS.seek 5ml C'3'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'v% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X  '4 +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'V'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'+'4 +506 h '4 C'.& h &W C'%% h %4 .t\CSV.dial :flush C'g  +38 h  .t\CS.seek 3ml C'6'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'{% h %0 .t\CS.seek 5ml C'I'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +506 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C'f'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'F& h &W C'&% h %4 .t\CSV.dial CSR C'9  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'p'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'k' +416 h ' ."connect"\CSV.dial CBV C'1 .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'd' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'm  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'w  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'Y'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'j  .t\CTV.dial CSV C'N  +38 h  +113  .t\CBV.dial :waste1 C'^ @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'E  +38 h  C'!% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '>'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'2  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C']  +37 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'P  +37 h +76 .t\CTV.dial :air C'A  +150 h .t\CS.seek 0ml C' '4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'h'0 +506 h '0 .t\CSV.dial :waste C'5 +38 h .t\CS.seek 0ml C'G'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'U% h %4 .t\CSV.dial :flush C':  +37 h  .t\CS.seek 5ml C'0'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +37 h @ C'_% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'n +113 h .t\CTV.dial :air .\CS.seek 0ml C'b'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +507 h '0 .t\CSV.dial :waste C'~ +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'a& h &W C'% h %4 .t\CSV.dial :flush C'R  +37 h  .t\CS.seek 3ml C'c'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'?% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'Z +76 h .t\CS.seek 0ml C'-'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'@% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  '  +38 h  +112  .t\CBV.dial :waste1 C'L @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'O% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +37 h @ C'S% h %p .t\CS.seek 7ml C't'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'B  +38 h  .t\CBV.dial :waste2b C''  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'e& h &W C's% h %4 .t\CSV.dial CSR C',  +38 h  C'=% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'H'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'W' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'* '"  +38 h +75 .t\CTV.dial :intake C'/  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'y' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'7'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1372 h ' .t\CSV.dial CBV C'v +113 h .t\CS.seek 0ml C'3'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&X& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&.& h &ӷ M&%% h % .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &+(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&I1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'z& h & .tHand.open M&F1 +2 h 1 M&&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &9(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.seek :garage M&p'η +91 h 'η .tHand.close M&1/ +150 h / M&& h &- M&k% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +265 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'Y(W +416 h (W C'j& h &W .tHand.open M&N1 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'!% h %4 .t\CSV.dial CSR C'  +113 h  C'>% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'o'[ +463 h '[ .t\CSV.dial :waste C'] +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +2009 h '4 #t\Purging external Intake C'& h &W C'}% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A +75 h .t\CTV.dial :intake C'(  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'5  +75 h #t\Flushing upper manifold C'h& h &W C'% h %4 .t\CSV.dial :flush C'U  +75 h  .t\CS.seek 5ml C':'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +37 h @ C'q% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'0 +113 h .t\CTV.dial :air .\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'b +37 h .t\CS.seek 0ml C'~'4 +507 h '4 C'8& h &W C'a% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'R'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C')% h %0 .t\CS.seek 5ml C'?'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'Z'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&M& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C' ( +416 h ( M&i& h &ӷ M&K% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &L(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:01 #\Preparing to sleep until Sun Sep 23 06:01:01 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST23-Sep-18 #\Continuing at 06:01CEST23-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:22:30CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST23-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2109h25ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*B  +22 h ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*,% h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *C'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*%'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*I% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*g  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ARV.dial :carrier F*w  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*>  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*3' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*2  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*]' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*o  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*}'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*P  +107 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*( n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*5'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*0  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2111h25ml.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*r% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*B'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*C  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*. I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*4' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +108 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*F'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*& n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*k  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*p% h % \\ ."MFBPCR"\AS.seek 0.326ml F*w'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2113h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*m  +21 h ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*N% h %Z ."MFBPCR"\AS.seek 0.81ml F*>'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +65 h  F*P% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*0% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*b  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*M  +10 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*O% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*S I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*' % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*B'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +107 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*D % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2116h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*v % +87 h % F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*I  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *$'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*j% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ARV.dial :carrier F*o  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*5  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*U n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*l  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*_' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*:'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*b  +22 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*~'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*c  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2118h25ml.pcr F*[% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*i  +22 h ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\ 'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*Q% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*'  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F**  +32 h  ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +21 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*X'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ARV.dial :carrier F*V  +11 h  F*$% h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +21 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*p I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*T % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*m  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*^% h %< ."MFBPCR"\AS.seek 0.594ml F*!'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*N n +21 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*3  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*P'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*J % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*] @@T ."MFBPCR"\ASV.dial :SPEdelivery F*}  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*(  +151 h F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *5' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*G  +43 h  F*h% h % ."MFBPCR"\AS.seek 0.62ml F*U'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*0 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *l'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*n'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*q % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *8' +424 h ' ."MFBPCR"\ATV.dial :air3 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*:% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*~% h % H ."MFBPCR"\AS.seek 0.735ml F*?' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +21 h F*R% h % Ű ."MFBPCR"\AS.seek 0.72ml F* ' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*c n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*@% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*M n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*[ % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %  ."MFBPCR"\AS.seek 0.604ml F*\ '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*O% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*-' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +86 h n ."MFBPCR"\ASV.dial :wasteR F*`  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*B% h % ."MFBPCR"\AS.seek 0.58ml F*e' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*,% h % q ."MFBPCR"\AS.seek 0.605ml F**'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*"% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*=' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*/  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*C  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*v n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *W'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*7 n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +202 h 'R ."MFBPCR"\SPE.coast F*<  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*.  +43 h  F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *;' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*I  +43 h  F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*$  +65 h  F*{% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *z'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*& n +65 h n F*g% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*1 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*F  +42 h  F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*6  +64 h  F*T% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *9'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*+ n +65 h n F*m% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *j' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*^  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *w': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*N n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *E'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*! n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*>  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*o'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %0 ."MFBPCR"\AS.seek 0.985ml F*P' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*A% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *0'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*l  +43 h  F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *n' +9988 h ' ."MFBPCR"\ATV.dial AIV F*_ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*8' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +129 h n ."MFBPCR"\AS.seek 0ml F*:'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*b% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*~  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +21 h F*c% h %Z ."MFBPCR"\AS.seek 0.81ml F*@'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +65 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*- % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*H  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' @CEST1537567247.20 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *W'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*X % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast P%6 M&5 +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537675261.78 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ). h  C' '# h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *;  h  .t\SPE.configure SPEconfig F*\ 2 *%% h %V .t\AS.configure ASconfig S)I% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X( +345 h ' +13 (  .t\Forearm.seek :retract M&'\ +6 h '\  .t\Elbow.seek :hammer M&3(_ + h '4 +92 (_ .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &' +91 h ' M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  M&2% h %\  P%J& C'}& h &2 ."openPC"\PC.reconfigure PCconfig h &3 ."openPC"\PC.home.seek :open .t\CC.reconfigure CCconfig P%$x2 ^22b %o( .t\CC.home.seek :open C'$x2 ^22b '(( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 06:01:17 began in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:23:28CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #6) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&P1 +150 h 1 M& & h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &G(- +165 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &0'η +91 h 'η .tHand.close M&U/ +150 h / M&l& h &- M&q% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &a' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'8& h &W .tHand.open M&:1 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C')& h &W C'% h %4 .t\CSV.dial CSR C'?  +150 h  C'c% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'R'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'M'[ +463 h '[ .t\CSV.dial :waste C'@ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '['4 +2010 h '4 #t\Purging external Intake C'r& h &W C'% h %4 .t\CSV.dial :flush C'Y  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'i 'd  h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'Q'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'O% h %4 .t\CSV.dial :flush C'\  +75 h  .t\CS.seek 5ml C'`'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +506 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'B'4 +507 h '4 C't& h &W C'% h %4 .t\CSV.dial :flush C',  +37 h  .t\CS.seek 3ml C's'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +37 h @ C'e% h %0 .t\CS.seek 5ml C'"'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'*'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +506 h '0 .t\CSV.dial :waste C'/ +76 h .t\CS.seek 0ml C'='4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'<( +416 h ( M&7& h &ӷ M&X% h % .t\Forearm.seek :retract M&W'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\\'η +91 h 'η .tHand.open M&1 +150 h 1 M&Z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:23:32CEST30-Aug-18\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:23:28CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C']& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&.% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(#% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (;& h &  M&%& h &- M&I% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &4( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F*$ % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *{' +160 h ( M&V& h & A(&& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &g' +89 h ' A(z& h &  .t\SE.seek 5.71 Pucks below Top Plate A(1' +205 h ' A(& h &  M&f& h & .t\Elbow.seek 2 M&F( +7 h ( M&p& h & A(T& h &  .t\Forearm.seek 2 + 300 counts M&k'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down A(6'X M&m& h & M&9% h %; .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.seek CC M&!(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +28 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'J(W +416 h (W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'A& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'  +37 h  .t\CS.seek 1.275ml C'' +920 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'   '9 h 9d +100 C'h9 h 9d +100 C'59 h 91d +100 C'P9 h 9J d +100 C'l9 h 9bd +100 C'{9 h 9zd +100 C'q9 h 9d +100 C'9 h 9d +100 C'U9 h 9 d +100 C'_9 h 9$d +100 C'n9 h 9(d +100 C'G9 h 9 ,d +100 C'39 h 9$0d +100 C'(9 h 9<4d +100 C'89 h 9S8d +100 C':9 h 9kd +100 C'O9 h 9Td +100 C'9 h 9kd +100 C'\ 9 h 9d +100 C'Q9 h 9d +100 C'9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'S9 h 9d +100 C'9 h 9 d +100 C'[9 h 9 d +100 C'9 h 96d +100 C''9 h 9Ld +100 C't9 h 9bd +100 C'9 h 9xd +100 C'-9 h 9d +100 C'B9 h 9d +100 C'H9 h 9d +100 C'e9 h 9d +100 C',9 h 9d +100 C's9 h 9d +100 C'*9 h 9d +100 C'/9 h 9(d +100 C'9 h 9>d +100 C'"9 h 9Td +100 C'9 h 9id +100 C'u9 h 9d +100 C'C9 h 9d +100 C'y9 h 9d +100 C' 9 h 9d +100 C' 9 h 9d +100 C'b9 h 9d +100 C'X9 h 9  d +100 C'L9 h 9 d +100 C'9 h 9 *d +100 C'v9 h 9 ?d +100 C'|9 h 9 Td +100 C'9 h 9 j d +100 C'79 h 9 $d +100 C'D9 h 9 (d +100 C'W9 h 9 ,d +100 C'9 h 9 0d +100 C'.9 h 9 4d +100 C'#9 h 9 8d +100 C'9 h 9  @ +37 h @ .t\CS.seek 5ml C''p +1373 h 'p .t\CSV.dial CTV C'h  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'5 @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'P  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'l @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'{'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'q +113 h .t\CBV.dial CSV C'  +19 h C'U% h %4 .t\CS.seek 5ml C'_'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'n  +38 h C'G% h %p .t\CS.seek 7ml C'3'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'( @ +38 h @ .t\CS.seek 10ml C'8' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C': ` +132 h ` #tLysing Complete C'~& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&)% h %\  A(% h %X A(c. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (a( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M& & h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &?( +142 h ( M&@& h & A(M& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &r' +90 h ' A(& h & .t\SE.seek 13.71 Pucks below Top Plate A(Y'n +125 h 'n M&R& h & A(d& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &i'Y +25 h 'Y .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(K- M&$&.,(\ #b h -+n .t\SE.seek 14.04 Pucks below Top Plate A(=' +27 h ' M&O% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&\ 'υ +21 h 'υ M&Q& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &`' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(' +36 h ' M&S& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&-/ +150 h / .t\CC.seek :open C'B( +416 h ( A(H& h & A(e& h & M&,& h &ӷ M&s% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &/( +143 h ( M&& h & A("& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +92 h 'Y .t\Elbow.hold M&u, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&y% h %Y A( & h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + & 'υ +21 h 'υ M&b& h & A(X& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &L' +22 h ' A(% h % ."downElevator"\SE.seek :down A(v'X +100 (|% h %- M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'D @ +19 h @ .t\CS.seek 10ml C'W' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'. { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'#& h & .tHand.open M&1 +2 h 1 M&& h & M&;% h %\  .t\Elbow.seek FlushPuck::Garage M&Z(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&$'η +90 h 'η .tHand.close M&]/ +150 h / M&&& h &- M&\\% h %η .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'& h &W .tHand.open M&+1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +150 h P%m% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%4' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%9' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%^' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'1  P%k  h +76 .t\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%j  h .t\PS.seek 0ml P%6' +2609 h ' .t\PSV.dial :air P%}  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%A' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%N' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&>& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +6 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&P' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C'( +416 h ( M&q& h &ӷ M&% h % .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &{(- +264 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &_'η +91 h 'η .tHand.open M&(1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C':& h & M&~% h %\  A()% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (c( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M& & h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &@( +166 h ( M&M& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &!' +90 h ' A(R& h &  .t\SE.seek 4.71 Pucks below Top Plate A(d' +214 h ' A(K& h &  M&O& h & .t\Elbow.seek 2 M&( +7 h ( M&Q& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&w'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ +150 h / ."downElevator"\SE.seek :down M&& A(r'X h & M&?% h %; .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.seek CC M&i(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +19 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\ ' +91 h ' .t\CC.seek :closed C'=(W +416 h (W .tHand.open M&`1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'[  +151 h  .t\CSV.dial CTV C'J  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'e' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C','4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%s  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%" +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%C  +151 h  .t\PS.seek :empty P%y' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'b  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'X @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'* ` +19 h ` .t\CSV.dial :air C'/ @ +38 h @ .t\CS.seek 10ml C'|' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'D'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'.  +150 h C'#& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(% h %X A(;. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&& h &ӷ M&L% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &I( +143 h ( M&x& h & A(v& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &a' +89 h ' A(8& h & .t\SE.seek 14.71 Pucks below Top Plate A(u'] +116 h '] M&]& h & A(&& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &\\'Y +26 h 'Y .t\Elbow.hold M&7, +6 h , .t\Elbow.reconfigure TightElbow A($- M&$&.,(\ #b h -+] .t\SE.seek 15.04 Pucks below Top Plate A(' +27 h ' M&f% h %Y A(Z& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&z'υ +20 h 'υ M&B& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &+' +22 h ' .t\SE.seek 16.04 Pucks below Top Plate A(%' +37 h ' M&p& h & M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  .t\Elbow.seek CC M&g(ӷ +143 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&9' +90 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'1( +416 h ( A(F& h & A(j& h & M&^& h &ӷ M&}% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&<& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &A'Y +92 h 'Y .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &N1 +150 h 1 M&>% h %Y A(h& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &5'υ +21 h 'υ M&l& h & A(V& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &E' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(q'X +100 (% h %X M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%G  +113 h  P%2% h % .t\PS.seek 0.499ml P%(' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%U  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%:  +37 h .t\CBV.dial AIV C'~  +38 h .t\PRV.dial between CBV and air P%)  +19 h  .t\PRV.dial :air P%  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %_' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&1 +2 h 1 M&R& h & M&{% h %\  .t\Elbow.seek FlushPuck::Garage M&3(- +166 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&Q'η +91 h 'η .tHand.close M&/ +150 h / M&S& h &- M&% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +264 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'w(W +416 h (W C'd& h &W .tHand.open M&1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%` @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%[  +75 h  .tdelay 2 seconds +200 P%J% h % .t\PS.seek 1.499ml P%\ ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%c' +1094 h ' .t\PSV.dial :air P%W  +38 h  .t\PS.seek 0ml P%H' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%e +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%s  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %"'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%C' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%b  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%X  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%* +75 h .t\PTV.dial :PRV,avoiding: :puck P%/ @ +76 h @ .t\PS.seek 0ml P%,' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%|' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%.' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%#  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%;  +75 h P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'L & +50 h & .t\AWV.select :waste C' +7 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %v'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'8 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %i' +1313 h ' .t\PSV.dial :air P%]  +37 h  .t\PS.seek 2.5ml P%&' +2610 h ' .t\PSV.dial :PML P%$ +38 h .t\PS.seek 0ml P%f' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'Z& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'B& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*-  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +21 h  ."MFBPCR"delay 20 seconds +92 h P%m% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*I I +129 h I F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *\\'6 +66 .t\PSV.dial :waste1 P%4  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%7' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*k  +22 h ' .t\PSV.dial PSR P% +21 h  F*=% h %6 ."MFBPCR"\AS.seek 0.329ml F*F'ͯ +92 h P%j% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%^' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*} % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%9' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*g  +22 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *>' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%h +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%l' +217 ."MFBPCR"\ATV.dial :AIV F*V I +42 h I F*T% h % ."MFBPCR"\AS.seek 0.469ml F*6' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%P +113 h P%A% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%5' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +38 h  .t\PS.seek 0ml P%G' +739 h ' ."MFBPCR"delay 5 seconds +500 F*29 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*E n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*q% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%:' +2610 h ' .t\PSV.dial PSR P%~ +112 h P%)% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%(' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %R'k +2679 h 'k .t\PSV.dial :waste1 P%{  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %K' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*O I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%S' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %n' +5106 h ' .t\PSV.dial :air P%Q  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %!' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +43 h  F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *?'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*1 I +43 h I F*d% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%r +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +619 h ' ."MFBPCR"\ATV.dial :waste F*` n +129 h n ."MFBPCR"\AS.seek :empty F*t'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*[% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *J' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*_  +43 h F*\ % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *W'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'c  +38 h  C'e% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C's'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'"'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*C% h %_ ."MFBPCR"\AS.seek 0.434ml F*b'ݏ +110 h '4 .t\CSV.dial CSR C'X  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'*'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*/  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*, n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*|'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'#' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C';& h &W #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C'v( +416 h ( M&]& h &ӷ M&i% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +264 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &-'η +91 h 'η .tHand.open M&p1 +150 h 1 M&m% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'I& h & M&u% h %\  A(4% h %X A(0. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (a( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (z& h &  M&& h &- M&+% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&k& h & A(=& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(j& h &  ."WCR"\SE.seek 3.71 Pucks below Top Plate A(\\' +223 h ' A(7& h &  M&}& h & ."WCR"\Elbow.seek 2 M&F( +7 h ( M&& h & A(g& h &  ."WCR"\Forearm.seek 2 + 300 counts M&N'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +31 h '&0 ."MFBPCR"delay 3 seconds +119 h / ."downElevator"\SE.seek :down M&h& A(<'X h & M&V% h %; ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.seek CC M&9(ӷ +90 ."MFBPCR"\ATV.dial :waste2 F*> % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&l& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &A' +91 h ' ."WCR"\CC.seek :closed C'(W +415 h (W ."WCR"Hand.open M&%1 +43 h 'R ."MFBPCR"\ATV.dial :air2 F*5  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *2' +85 h 1 M&E% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +7 h + S)y% h % C'G& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'U  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*l n +108 h n ."MFBPCR"\ASV.dial :wasteR F*P% h %  ."MFBPCR"\AS.seek 0.09ml F*N' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*F I +86 h I F*|% h % ."MFBPCR"\AS.seek 0.12ml F*<' +625 h ' ."MFBPCR"\ATV.dial :air3 F*T % +21 h % F*v% h % ."MFBPCR"\AS.seek 0.13ml F*5' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*E% h % ."MFBPCR"\AS.seek 0.23ml F*y' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*U'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*a% h %X ."MFBPCR"\AS.seek 0.24ml F*6' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*) n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*~  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*{  +42 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *O'\  +1032 h '\  F*M% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*K% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*R% h %\  ."MFBPCR"\AS.seek 0.5ml F*n'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*'% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *3' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +3472 h 'V ."MFBPCR"\SPE.coast F*Q  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*d  +64 h  ."MFBPCR"\ARV.dial :carrier F*r  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*!'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*_  +21 h ."MFBPCR"delay 2 seconds +200 F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*J  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*e% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*s  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*D' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +107 h  ."MFBPCR"delay 7 seconds +700 F*X% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*.% h % \\ ."MFBPCR"\AS.seek 0.326ml F*#'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254689 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'L  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'; @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'@ @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C'Y ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'i  'Z  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'p  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'B' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'$  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'm  +38 h  C'u% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'0' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'I @ +75 h @ +50 S)4% h % ."WCR"\CBV.dial between CSV and waste2b C'+  +94 h  C'-% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'=' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'j  'k  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'f'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'7  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '&' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'g  +38 h  C'}% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'^' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'V  +94 h  C'l% h % ."WCR"\CSV.dial between air and CTV C'P ` +56 h ` ."WCR"\CS.seek 7.288ml C'>' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'\\  ."WCR"\CSV.dial CTV C'F  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '|'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'N  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'T' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'v  +151 h C'<& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&%% h %\  A(E% h %X A(5. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (G( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&y& h &ӷ M&A% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &a( +142 h ( M&U& h & A()& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +90 h ' A(6& h & ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +106 h 'L M&:& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &'Y +26 h 'Y ."WCR"\Elbow.hold M&(, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+L ."WCR"\SE.seek 16.04 Pucks below Top Plate A({' +27 h ' M&% h %Y A(2& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &M'υ +20 h 'υ M&O& h & A(K& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' ."WCR"\SE.seek 17.04 Pucks below Top Plate A(R' +36 h ' M&& h & M&'% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&3& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ ."WCR"\Forearm.seek CC M&d' +91 h ' ."WCR"Hand.close M&r/ +150 h / ."WCR"\CC.seek :open C' ( +416 h ( A(S& h & A(!& h & M&& h &ӷ M&`% h % ."WCR"\Forearm.seek :retract M&'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &1( +143 h ( M&_& h & A(\ & h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &['Y +92 h 'Y ."WCR"\Elbow.hold M&J, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &e1 +150 h 1 M&?% h %Y A(s& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&H'υ +20 h 'υ M&c& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(D% h % ."downElevator"\SE.seek :down A(C'X +93 h 'X ."downElevator"\SE.coast A(5 +7 (X% h %X M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'"& h & .tHand.open M& 1 +2 h 1 M&& h & M&/% h %\  .t\Elbow.seek FlushPuck::Garage M&W(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&./ +150 h / M&,& h &- M&*% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &@' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'w& h &W .tHand.open M&]1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Z& h &W C'i% h % .t\CSV.dial CSR C'  +37 h  C'p% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '$% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'm'[ +463 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'B'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'0& h &W C'I% h %4 .t\CSV.dial :flush C'4  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '+'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'% h %p .t\CS.seek 7ml C'z'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'k  'j  +38 h  +113  .t\CBV.dial :waste1 C'= @ +75 h @ .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'f& h &W C'% h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +38 h @ C'^% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'V  +75 h  .t\CS.seek 0ml C'l'4 +766 h '4 #t\Flushing upper manifold C'P& h &W C'h% h %4 .t\CSV.dial :flush C'F  +75 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'N% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'< 'v  +38 h +75 .t\CTV.dial :air C'%  +151 h .t\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'y'4 +506 h '4 C'A& h &W C'U% h %4 .t\CSV.dial :flush C')  +38 h  .t\CS.seek 3ml C'6'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C':% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'2 +75 h .t\CS.seek 0ml C'O'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'K& h &W C'q% h %4 .t\CSV.dial CSR C'  +150 h  C''% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'~'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'a' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'3' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Q  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'{  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'('P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'n  +38 h  +113  .t\CBV.dial :waste1 C'S @ +37 h @ .t\CS.seek 0ml C'!'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'`% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\ ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'e'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'?  +38 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'c'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D  ' +38 h +75 .t\CTV.dial :air C'1  +151 h .t\CS.seek 0ml C'X'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'" +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C'& h &W C'/% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'b% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'C +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +506 h '0 .t\CSV.dial :waste C'J +38 h .t\CS.seek 0ml C'.'4 +507 h '4 C',& h &W C'*% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'% h %0 .t\CS.seek 5ml C'W'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'w +76 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'Y& h &W C'Z% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +38 h @ C'@% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV C'$  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'u @ +38 h @ .t\CS.seek 0ml C'm'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'0& h &W C'I% h %4 .t\CSV.dial :flush C'4  +75 h  .t\CS.seek 5ml C'B'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +37 h @ C'% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'k  +38 h  .t\CBV.dial :waste2b C'j  +75 h  .t\CS.seek 0ml C'='4 +766 h '4 #tDisinfecting external Intake with kill C'z& h &W C'f% h %4 .t\CSV.dial CSR C'  +38 h  C'g% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'7'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'&' +416 h ' ."connect"\CSV.dial CBV C'^ .t\CBV.dial CSV C'}  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'V' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'P'4 +607 h '4 .t\CSV.dial CSR C'h  +113 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'>' +1372 h ' .t\CSV.dial CBV C'N +113 h .t\CS.seek 0ml C'\\'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&v& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +7 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&E' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&A& h &ӷ M&U% h % .t\Forearm.seek :retract M&)'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +265 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&K1 +2 h 1 M&q& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&a% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +265 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +90 h ' .t\CC.seek :closed C'((W +416 h (W C'& h &W .tHand.open M&n1 +150 h 1 M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'`% h %4 .t\CSV.dial CSR C'!  +113 h  C'_% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '\ '[ +463 h '[ .t\CSV.dial :waste C'? +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 's'4 +2009 h '4 #t\Purging external Intake C'e& h &W C'c% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'1 .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'X'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'"  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'/  +75 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'R% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'J'4 +507 h '4 C'G& h &W C',% h %4 .t\CSV.dial :flush C'*  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +38 h @ C';% h %0 .t\CS.seek 5ml C''p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'w'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&p/ +150 h / .t\CC.seek :open C'@( +416 h ( M&& h &ӷ M&$% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &I'η +91 h 'η .tHand.open M&41 +150 h 1 M&m% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:17 #\Preparing to sleep until Tue Sep 25 06:01:17 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST25-Sep-18 #\Continuing at 06:01CEST25-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:24:58CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST25-Sep-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2309h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*k  +22 h ."MFBPCR"\ATV.dial :air3 F*j % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +65 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *F'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*U'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*)  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*{  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *!'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*_  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*?' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*c'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +108 h  ."MFBPCR"delay 7 seconds +700 F*s% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*"'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*['{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2311h25ml.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*G % +87 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*W% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*i  +65 h  F*p% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*k'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*B'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*f  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ARV.dial :carrier F*F  +10 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*l'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*> n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*A I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*8% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*9 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*O'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*a  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*~% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2313h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +21 h F*n% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*\\% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +65 h  F*D% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*"  +32 h  ."MFBPCR"\ATV.dial :air3 F*X % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*3% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*0  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*j % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*k'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*B% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*z n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*P % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2316h25ml.pcr F*h% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*|'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F*E'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +64 h  F*<% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*O % +86 h % F*a% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*5  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*s% h %V ."MFBPCR"\AS.seek 0.5ml F*c'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*"  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*X  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*/ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*. n +22 h n ."MFBPCR"delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*W  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*3 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2318h25ml.pcr F*i% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *0'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*#% h %n ."MFBPCR"\AS.seek 0.82ml F*m'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*j  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *f'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +31 h  ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*F'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +21 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +21 h F*N% h %m ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ARV.dial :carrier F*E  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +22 h ."MFBPCR"delay 2 seconds +200 F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*~ I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*2 % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +21 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*!  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*e% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*s % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*? @@T ."MFBPCR"\ASV.dial :SPEdelivery F*c  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *"' +424 h ' ."MFBPCR"\ATV.dial :ASV F*X n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*/'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b * 'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *G' +424 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*[% h % ."MFBPCR"\AS.seek 0.71ml F*.' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*W' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*Z% h % zG ."MFBPCR"\AS.seek 0.365ml F* ' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*i % +43 h % F*p% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*$% h % ."MFBPCR"\AS.seek 0.579ml F*r' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %  ."MFBPCR"\AS.seek 0.604ml F*0'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*I% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*]' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*#  +21 h  ."MFBPCR"\AS.seek 0ml F*T'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*m% h %V ."MFBPCR"\AS.seek 0.01ml F*f' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +21 h F*k% h % ."MFBPCR"\AS.seek 0.58ml F*z' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*^'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*}% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*g' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*V n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *h'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*F  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *>' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*N n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *&'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*l n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +201 h 'R ."MFBPCR"\SPE.coast F*L  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*%'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*A  +43 h  F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *|' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*9 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *E': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*:% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*q n +64 h n F*)% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *'' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*K  +43 h  F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*2% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*y n +64 h n F*Q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*S  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *{': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*n n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.seek 0ml F*!'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*_  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*M % +43 h % F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*d% h % ."MFBPCR"\AS.seek 0.96ml F*\ '0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*c% h %0 ."MFBPCR"\AS.seek 0.985ml F*D' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*1% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*X I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *C' +9988 h ' ."MFBPCR"\ATV.dial AIV F*b I +43 h I ."MFBPCR"\AS.seek 0.02ml F*G' +9880 h ' ."MFBPCR"\ATV.dial :waste F*, n +128 h n ."MFBPCR"\AS.seek 0ml F*['V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*Z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*#  +32 h  ."MFBPCR"\ATV.dial :air3 F*] % +86 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *j' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*f'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds @CEST1537740002.88 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *&'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*v % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2548 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + &6 C'5 P%5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537848077.22 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )A h  C' '8 h  P% %% h  F* *9 h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *|  h  .t\SPE.configure SPEconfig F*\ 2 *U% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'<& S)#.0X 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:25:55CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #7) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&D1 +150 h 1 M&X& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &H'η +91 h 'η .tHand.close M&// +150 h / M& & h &- M&R% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'G& h &W .tHand.open M&[1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C';& h &W C'*% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'Z +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +2010 h '4 #t\Purging external Intake C'p& h &W C' % h %4 .t\CSV.dial :flush C'(  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '$' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '3  h +75 .t\CTV.dial :intake C'Y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'4'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'r  +75 h #t\Flushing upper manifold C'& h &W C'I% h %4 .t\CSV.dial :flush C'0  +75 h  .t\CS.seek 5ml C'#'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'T% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'B +37 h .t\CS.seek 0ml C'k'4 +507 h '4 C'+& h &W C'=% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'f'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'z% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'^'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'g'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.seek CC M&N' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'L( +416 h ( M&l& h &ӷ M&v% h % .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&u& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&o1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:26:00CEST30-Aug-18\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:25:55CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*w% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'?& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&A% h %\  A(8% h %X A(%. h ., A(9& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (|& h &  M&U& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &<( +6 F*x% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *:' +160 h ( M&E& h & A(q& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &)' +89 h ' A(& h &  .t\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') A('& h &  M&O& h & .t\Elbow.seek 2 M&K( +7 h ( M&~& h & A(6& h &  .t\Forearm.seek 2 + 300 counts M&a'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +150 h / ."downElevator"\SE.seek :down M&2& A(Q'X h & M&% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&`(ӷ +142 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +2 h 'X ."downElevator"\SE.coast A(5 +89 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&c1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)M+ +7 h + S)!% h % C'1& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +387 h ' +50 .t\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'I9 h 9Td +100 C'Y9 h 9kd +100 C'09 h 9d +100 C'49 h 9d +100 C'T9 h 9d +100 C'#9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'm9 h 9 d +100 C'i9 h 9 d +100 C'9 h 96d +100 C'j9 h 9Ld +100 C'+9 h 9bd +100 C'=9 h 9xd +100 C']9 h 9d +100 C'k9 h 9d +100 C'79 h 9d +100 C'z9 h 9d +100 C'9 h 9d +100 C'f9 h 9d +100 C'^9 h 9d +100 C' 9 h 9(d +100 C'9 h 9>d +100 C'}9 h 9Td +100 C'h9 h 9id +100 C'B9 h 9d +100 C'F9 h 9d +100 C'V9 h 9d +100 C'>9 h 9d +100 C'.9 h 9d +100 C'9 h 9d +100 C'v9 h 9  d +100 C'u9 h 9 d +100 C'9 h 9 *d +100 C'N9 h 9 ?d +100 C'-9 h 9 Td +100 C'o9 h 9 j d +100 C'l9 h 9 $d +100 C'P9 h 9 (d +100 C'&9 h 9 ,d +100 C'w9 h 9 0d +100 C'A9 h 9 4d +100 C'89 h 9 8d +100 C'%9 h 9 & h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&.'υ +20 h 'υ M&& h & A(v& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &u' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +84 h 'X ."downElevator"\SE.coast A(5 +16 (-% h %X M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'P @ +19 h @ .t\CS.seek 10ml C'&' +6 h ' .t\CSV.dial between air and CTV C'w ` +19 h ` .t\AIV.dial :bypass C'A { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'8& h & .tHand.open M&%1 +2 h 1 M&9& h & M&|% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&?/ +150 h / M&q& h &- M&t% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +264 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'6(W +416 h (W C'& h &W .tHand.open M&y1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%~ +151 h P%2% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%)  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%e +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%S' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'a  h +75 .t\PSV.dial :mixloop P%K +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%Q  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%c  +37 h  .t\PS.seek 2.5ml P%L' +2610 h ' .t\PSV.dial :mixloop P%! +38 h .t\PS.seek 0ml P%1' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%M' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&_& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&D' +90 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( M&R& h &ӷ M&d% h % .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +265 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +91 h 'η .tHand.open M&1 +150 h 1 M&X% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'[& h & M&J% h %\  A(;% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (5& h &  M&W& h &- M&*% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Z( +166 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &`' +89 h ' A(& h &  .t\SE.seek 1.71 Pucks below Top Plate A(3': +244 h ': A($& h &  M&I& h & .t\Elbow.seek 2 M&Y( +7 h ( M&4& h & A(T& h &  .t\Forearm.seek 2 + 300 counts M&{'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&m& A(p'X h & M&% h %; .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'g(W +416 h (W .tHand.open M +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'i  +151 h  .t\CSV.dial CTV C's  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'z' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%f  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%} +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%=  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%F  +150 h  .t\PS.seek :empty P%V' +2610 h ' .t\CBV.dial :waste1 C'> @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'v @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'^ ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'-' +2647 h ' .t\CSV.dial CTV C'o  +38 h  .t\CS.seek 0ml C'P'4 +2647 h '4 .t\CBV.dial :waste1 C'w @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'A  +150 h C'8& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&%% h %\  A(9% h %X A(|. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (h( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (.& h & M&& h &ӷ M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &u( +142 h ( M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(G& h & .t\SE.seek 17.71 Pucks below Top Plate A(B'* +87 h '* M&?& h & A(q& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &t'Y +25 h 'Y .t\Elbow.hold M&l, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+* .t\SE.seek 18.04 Pucks below Top Plate A(@' +26 h ' M&'% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&k& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' .t\SE.seek 19.04 Pucks below Top Plate A(U' +36 h ' M&~& h & M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +89 h '\  .t\Elbow.seek CC M&)(ӷ +143 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'a( +416 h ( A(K& h & A(Q& h & M&S& h &ӷ M&c% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &!( +142 h ( M&L& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &1'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &n1 +150 h 1 M&_% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &"'υ +21 h 'υ M& & h & A(E& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(6% h % ."downElevator"\SE.seek :down A(R'X +73 h 'X ."downElevator"\SE.coast A(5 +27 (d% h %X M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +89 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%X +37 h .t\PRV.dial :air P%/  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%[  +38 h .t\CBV.dial AIV C'J  +37 h .t\PRV.dial between CBV and air P%;  +19 h  .t\PRV.dial :air P%  +19 h  P%5% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %W' +250 h ' .t\PRV.dial :waste2 P%* @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %b' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M& 1 +2 h 1 M&& h & M&:% h %\  .t\Elbow.seek FlushPuck::Garage M&\\(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.seek :garage M&4'η +91 h 'η .tHand.close M&T/ +150 h / M&& h &- M&m% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +265 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +90 h ' .t\CC.seek :closed C'3(W +416 h (W C'{& h &W .tHand.open M&Y1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%# @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%r  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%i  +75 h  .tdelay 2 seconds +200 P%s% h % .t\PS.seek 1.499ml P%0' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%&  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%z +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%f  +38 h  .tdelay 2 seconds +200 P%M% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %}'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%=  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%F' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%>'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%v  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%^ +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%o  +75 h  .t\PS.seek 2.5ml P%-' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%w +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%A' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%8  +75 h  .t\PS.seek 2.5ml P%%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%9 +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%|  +76 h P%.% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'N & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %x'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'G { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%?  +38 h  .t\PS.seek 2.5ml P%q' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%'' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'k& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*]  .t\AIV.dial :bypass .\PSV.dial PSR P%~ +22 h  ."MFBPCR"delay 20 seconds +91 h P%2% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%,' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*u I +129 h I F*B% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *t'6 +66 .t\PSV.dial :waste1 P%<  +37 h  .t\PS.seek 0ml P%e' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%@' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%l' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%O +21 h  F*g% h %6 ."MFBPCR"\AS.seek 0.329ml F*K'ͯ +92 h P%Q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%S' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*c % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*)  +22 h  F*n% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *_' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +217 ."MFBPCR"\ATV.dial :AIV F*E I +43 h I F*6% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%! +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +2609 h ' .t\PSV.dial PSR P%D +113 h P%1% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%"' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +37 h  .t\PS.seek 0ml P% ' +740 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*R n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*h'% +336 h ' .t\PSV.dial PSR P%V +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%X' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%[' +2610 h ' .t\PSV.dial PSR P%J +113 h P%;% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%W' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%:  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %$' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*I I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%T  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%m +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %C' +5107 h ' .t\PSV.dial :air P%4  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %`' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*j  +42 h  F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*a I +43 h I F*{% h %Q ."MFBPCR"\AS.seek 0.01ml F*Y' +171 h ' .t\PSV.dial :mixloop P%( +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*# n +129 h n ."MFBPCR"\AS.seek :empty F*+'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*r  +86 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *s' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*b  +43 h F*0% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *&'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'z% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'f'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'M' +1625 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV C'=  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'}'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*F% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +109 h '4 .t\CSV.dial CSR C'v  +38 h  C'>% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'^'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*o'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*w% h %& ."MFBPCR"\AS.seek 0.092ml F*-'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'8' +1625 h ' ."connect"\CSV.dial CTV C'A  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'9'4 +2647 h '4 C'|& h &W #tGaraging FlushPuck in Collection Clamp M&.& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +6 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'x( +416 h ( M&?& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &q(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &]'η +91 h 'η .tHand.open M&~1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'u& h & M&B% h %\  A(<% h %X A(H. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (,( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&e& h &- M&y% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &@( +166 h ( M&& h & A(g& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &O' +89 h ' A(Q& h &  ."WCR"\SE.seek 0.71 Pucks below Top Plate A(t'K +254 h 'K A(l& h &  M&c& h & ."WCR"\Elbow.seek 2 M&K( +6 h ( M&& h & A()& h &  ."WCR"\Forearm.seek 2 + 300 counts M&n'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ + h '&0 ."MFBPCR"delay 3 seconds +149 h / ."downElevator"\SE.seek :down A(L'X M&& h & M&E% h %; ."WCR"\Forearm.seek :retract M&6'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +60 ."MFBPCR"\ATV.dial :waste2 F*_ % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +40 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &1' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' ."WCR"\CC.seek :closed C'd(W +416 h (W ."WCR"Hand.open M&U1 +13 h 'R ."MFBPCR"\ATV.dial :air2 F*"  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +115 h 1 M&R% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)V% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'/  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *&' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *}% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b * ' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*w'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*o n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*A % +43 h % F*8% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *|'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*. I +21 h I F*N% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*G % +21 h % F*Z% h %H ."MFBPCR"\AS.seek 0.069ml F*p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*?% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*k' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*~% h % k ."MFBPCR"\AS.seek 0.779ml F*2'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*B n +128 h n ."MFBPCR"\AS.seek :empty F*<'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*]% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %  ."MFBPCR"\AS.seek 0.604ml F*q'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*l% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*c' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*)  +43 h  ."MFBPCR"\AS.seek 0ml F*S'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *E'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*@ % +108 h % F*O% h %V ."MFBPCR"\AS.seek 0.59ml F*_' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +107 h n ."MFBPCR"\ASV.dial :wasteR F*D% h %  ."MFBPCR"\AS.seek 0.09ml F*t' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*K I +86 h I F*-% h % ."MFBPCR"\AS.seek 0.12ml F*6' +625 h ' ."MFBPCR"\ATV.dial :air3 F*L % +22 h % F*x% h % ."MFBPCR"\AS.seek 0.13ml F*"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*R% h % ."MFBPCR"\AS.seek 0.23ml F*V' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*!% h % ."MFBPCR"\AS.seek 0.255ml F*/'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*,% h %X ."MFBPCR"\AS.seek 0.24ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*;  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*X @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*h I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*:  +43 h  #"MFBPCR"\Swish #1 of 2 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *I'\  +1031 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*T'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*$% h %(A ."MFBPCR"\AS.seek 0.6ml F*m'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*C'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*j% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *\\' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*4  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2509h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*{  +65 h  ."MFBPCR"\ARV.dial :carrier F*(  +10 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*`'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*a  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*s  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*f  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*P' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*F  +107 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*>'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.6ml F*&'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*o  +64 h ."MFBPCR"\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*A% h % \\ ."MFBPCR"\AS.seek 0.326ml F*8'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254718 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'N  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'| @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'G' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'Z @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'3  +94 h  C'?% h % ."WCR"\CSV.dial between air and CTV C'p ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '%'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'~  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'2  +38 h  C'B% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'H' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'u @ +75 h @ +50 S)<% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C'e ` +57 h ` ."WCR"\CS.seek 7.288ml C'g' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Q  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'l  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'q' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C')  +38 h  C'c% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'S' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'@ @ +76 h @ +50 S)O% h % ."WCR"\CBV.dial between CSV and waste2b C'E  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C'D ` +56 h ` ."WCR"\CS.seek 7.288ml C'_' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'K  'n  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '-'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C't  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'x  +151 h C'6& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&U% h %\  A(R% h %X A(". h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ( ( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (!& h & M&V& h &ӷ M&1% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &,( +143 h ( M&/& h & A(J& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &;' +89 h ' A(& h & ."WCR"\SE.seek 18.71 Pucks below Top Plate A(5' +77 h ' M&[& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &h'Y +25 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A( - M&$&.,(\ #b h -+ ."WCR"\SE.seek 19.04 Pucks below Top Plate A(:' +27 h ' M&*% h %Y A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M& 'υ +21 h 'υ M&I& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &T' +22 h ' ."WCR"\SE.seek 20.04 Pucks below Top Plate A(' +36 h ' M&m& h & M&j% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&\\& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ ."WCR"\Forearm.seek CC M&{' +91 h ' ."WCR"Hand.close M&(/ +150 h / ."WCR"\CC.seek :open C'W( +416 h ( A(& h & A(`& h & M&Y& h &ӷ M&#% h % ."WCR"\Forearm.seek :retract M&r'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &a( +142 h ( M&b& h & A(0& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &i'Y +93 h 'Y ."WCR"\Elbow.hold M&s, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &z1 +150 h 1 M&% h %Y A(f& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&7'υ +21 h 'υ M&& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &M' +22 h ' A(P% h % ."downElevator"\SE.seek :down A(F'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (v% h %X M&=% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'}& h & .tHand.open M&>1 +2 h 1 M&& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&w'η +91 h 'η .tHand.close M&A/ +150 h / M&& h &- M&^% h %η .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +264 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'3& h &W .tHand.open M&?1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h % .t\CSV.dial CSR C'  +38 h  C'~% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'%'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '2'[ +463 h '[ .t\CSV.dial :waste C'B +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'H& h &W C'u% h %4 .t\CSV.dial :flush C'<  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'e% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'Q  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'g @ +75 h @ .t\CS.seek 0ml C'l'4 +766 h '4 #t\Flushing lower manifold's waste2b port C''& h &W C'% h %4 .t\CSV.dial :flush C')  +75 h  .t\CS.seek 5ml C'c'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +37 h @ C'S% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'O  +38 h  .t\CBV.dial :waste2b C'E  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing upper manifold C'D& h &W C'% h %4 .t\CSV.dial :flush C'n  +76 h  .t\CS.seek 5ml C'K'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C't% h %p .t\CS.seek 7ml C'-'i +415 h 'i ."connect"\CSV.dial CBV C'x .t\CBV.dial CSV C'6  +38 h +75 .t\CTV.dial :air C'U  +151 h .t\CS.seek 0ml C'R'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'"'0 +506 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'V'4 +507 h '4 C'1& h &W C'/% h %4 .t\CSV.dial :flush C'J  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'[% h %0 .t\CS.seek 5ml C''p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'I'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'$& h &W C'% h %4 .t\CSV.dial CSR C'm  +151 h  C'j% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C';'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C',' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'5 +113 h .t\CTV.dial :intake C'h  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'\\' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'4  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C':  +113 h  C'T% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'`'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'Y  +38 h  C'#% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'f% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C'P .t\CBV.dial CSV C'r  +38 h +75 .t\CTV.dial :air C'a  +150 h .t\CS.seek 0ml C'v'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +507 h '0 .t\CSV.dial :waste C'} +37 h .t\CS.seek 0ml C'>'4 +507 h '4 #t\Flushing upper manifold C'& h &W C' % h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'o'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'F +113 h .t\CTV.dial :air .\CS.seek 0ml C'M'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C's +38 h .t\CS.seek 0ml C'A'4 +506 h '4 C'& h &W C'^% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'w'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'9% h %0 .t\CS.seek 5ml C'&'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'?'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'p& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +37 h @ C'Z% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'%  +38 h  +113  .t\CBV.dial :waste1 C'B @ +37 h @ .t\CS.seek 0ml C'2'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'H& h &W C'u% h %4 .t\CSV.dial :flush C'<  +76 h  .t\CS.seek 5ml C'k'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'e% h %p .t\CS.seek 7ml C'y'i +415 h 'i ."connect"\CSV.dial CTV C'Q  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'g'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C''% h %4 .t\CSV.dial CSR C'  +38 h  C')% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'l'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'S 'c  +37 h +76 .t\CTV.dial :intake C'O  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'E' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'@  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'_' +1372 h ' .t\CSV.dial CBV C't +113 h .t\CS.seek 0ml C'K'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&6& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +7 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'"( +416 h ( M&1& h &ӷ M&/% h % .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&$1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &m(- +7 h (- M&j& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M&5/ +150 h / M&h& h &- M&,% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'C& h &W .tHand.open M&d1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'#% h %4 .t\CSV.dial CSR C'`  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'{'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '0'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'f'4 +2010 h '4 #t\Purging external Intake C'z& h &W C'% h %4 .t\CSV.dial :flush C'r  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'v'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'}  +75 h #t\Flushing upper manifold C'=& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 +113 h .t\CTV.dial :air .\CS.seek 0ml C'F'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +507 h '0 .t\CSV.dial :waste C'M +38 h .t\CS.seek 0ml C's'4 +506 h '4 C' & h &W C'% h %4 .t\CSV.dial :flush C'^  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'9'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'w'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +507 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C'3'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &p(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &u'η +91 h 'η .tHand.open M&<1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:32 #\Preparing to sleep until Thu Sep 27 06:01:32 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST27-Sep-18 #\Continuing at 06:01CEST27-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:27:26CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST27-Sep-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2509h25ml.pcr F*e% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*E  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*! % +86 h % F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*1% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2511h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ARV.dial :carrier F*:  +10 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F*m'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *`'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*b  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*K' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*0  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*r  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*v  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*>  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2511h25ml.pcr F*F% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*|'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*~% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*p % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*u% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2513h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*_ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*N  +21 h ."MFBPCR"delay 2 seconds +200 F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*1 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*x' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*h' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*,  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*;% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2513h25ml.pcr F*m% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*4  +21 h ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*0'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*r% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *f'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*}  +31 h  ."MFBPCR"\ATV.dial :air3 F*v % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*7% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +21 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*M  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2516h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*W  +10 h  F*\\% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*% n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*u% h %Fj ."MFBPCR"\AS.seek 0.524ml F*2' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*e I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*'  +108 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*)  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.65ml F*O' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*c  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*E% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*D % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2516h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*_  +22 h ."MFBPCR"\ATV.dial :air3 F*t % +87 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*U% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*x% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +31 h  ."MFBPCR"\ATV.dial :air3 F*I % +87 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*T'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*C% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *#'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2518h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*r  +42 h  ."MFBPCR"\ARV.dial :carrier F*0  +11 h  F*f% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*}  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*v  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*X  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*o  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*i'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*A n +21 h n ."MFBPCR"delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*~'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2518h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *H'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*l % +86 h % F*c% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *N'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2520h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ARV.dial :carrier F*R  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*5  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*I  +21 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*; I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*m% h % ."MFBPCR"\AS.seek 0.53ml F*T' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*4  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.536ml F*:'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*#'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*d n +22 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*`  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*K  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*0  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*f % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2520h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *}' +423 h ' ."MFBPCR"\ATV.dial :ASV F*v n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*>  +43 h  F*=% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*7 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *X'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*F'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*M' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*i% h % ."MFBPCR"\AS.seek 0.71ml F*A' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h % H ."MFBPCR"\AS.seek 0.735ml F*9' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*&' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*.' +7161 h ' ."MFBPCR"\ATV.dial :waste F*W n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\\'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*~% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*(' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %  ."MFBPCR"\AS.seek 0.604ml F*H'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*u% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*8  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*2% h %V ."MFBPCR"\AS.seek 0.01ml F*'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*S'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +21 h F*c% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*)' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*E n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*O  +21 h  ."MFBPCR"\AS.seek 0ml F*@'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*n  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *_' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *q'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +202 h 'R ."MFBPCR"\SPE.coast F*N  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*! n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*1  +42 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *-' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*[% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b **'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *j' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*5 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *h': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *m'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*V n +65 h n F*4% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *C' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*T I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *:': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*d n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *Y'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*# n +151 h n ."MFBPCR"\AS.seek 0ml F*`'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*b  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*K% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*{% h % ."MFBPCR"\AS.seek 0.96ml F*0'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*r' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*a% h % ."MFBPCR"\AS.seek 0.97ml F*P'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*v I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *='R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *7'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*X  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *F' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*i'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*M% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*s  +21 h ."MFBPCR"\ATV.dial :air3 F*A % +43 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*~% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*8  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*''m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V @CEST1537912815.32 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *_'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *q'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*6 % +108 h % ."MFBPCR"\AS.seek 0ml F*!'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538020892.66 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )1 h  C' 'G h  P% %U h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *-  h  .t\SPE.configure SPEconfig F*\ 2 */% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'x& S)#.0X 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:28:23CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #8) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'a& h & .tHand.open M&r1 +150 h 1 M&v& h &_ M&P% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &>(- +166 h (- M&=& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &7'η +91 h 'η .tHand.close M& / +150 h / M&X& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'M(W +416 h (W C' & h &W .tHand.open M&i1 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'|& h &W C'^% h %4 .t\CSV.dial CSR C'9  +151 h  C'w% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '&% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'W'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'~& h &W C'.% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'\\  h +76 .t\CTV.dial :intake C'p  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'<'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'(  +76 h #t\Flushing upper manifold C'%& h &W C'u% h %4 .t\CSV.dial :flush C'H  +75 h  .t\CS.seek 5ml C'8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +38 h @ C'L% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'2'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'k +38 h .t\CS.seek 0ml C'Q'4 +506 h '4 C'y& h &W C'g% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C'% h %0 .t\CS.seek 5ml C'c'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'S'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +507 h '0 .t\CSV.dial :waste C'O +75 h .t\CS.seek 0ml C')'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&@& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +6 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'N( +416 h ( M& & h &ӷ M&6% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &+'η +91 h 'η .tHand.open M&!1 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:28:28CEST30-Aug-18\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:28:23CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*3% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&1% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(G% h %X A(U. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (-& h &  M&/& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &x( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *[' +159 h ( M&R& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +90 h ' A(*& h &  .t\SE.seek -0.29 Pucks below Top Plate A(5'\\ +264 h '\\ A(j& h &  M&$& h & .t\Elbow.seek 2 M&I( +7 h ( M&;& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down M&,& A(4'X h & M&m% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&#(ӷ +142 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'f(W +416 h (W .tHand.open M&1 +150 h 1 M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +7 h + S)`% h % C'a& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X9 h 9 ,d +100 C'K9 h 9$0d +100 C'9 h 9<4d +100 C' 9 h 9S8d +100 C'i9 h 9kd +100 C'u9 h 9Td +100 C'9 h 9kd +100 C'H9 h 9d +100 C'<9 h 9d +100 C'L9 h 9d +100 C'89 h 9d +100 C'9 h 9d +100 C'e9 h 9d +100 C'29 h 9 d +100 C'9 h 9 d +100 C'%9 h 96d +100 C'9 h 9Ld +100 C'y9 h 9bd +100 C'g9 h 9xd +100 C'?9 h 9d +100 C'Q9 h 9d +100 C'l9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C''9 h 9d +100 C'S9 h 9d +100 C'O9 h 9(d +100 C'@9 h 9>d +100 C'c9 h 9Td +100 C'9 h 9id +100 C'k9 h 9d +100 C'n9 h 9d +100 C'E9 h 9d +100 C'_9 h 9d +100 C'A9 h 9d +100 C'M9 h 9d +100 C'69 h 9  d +100 C't9 h 9 d +100 C'o9 h 9 *d +100 C'9 h 9 ?d +100 C']9 h 9 Td +100 C'!9 h 9 j d +100 C' 9 h 9 $d +100 C'D9 h 9 (d +100 C'q9 h 9 ,d +100 C'39 h 9 0d +100 C'19 h 9 4d +100 C'G9 h 9 8d +100 C'U9 h 9 % h %p .t\CS.seek 7ml C'K'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +37 h @ .t\CS.seek 10ml C' ' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'i ` +132 h ` #tLysing Complete C's& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&|% h %\  A(% h %X A(w. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ("& h & M&&& h &ӷ M&^% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &9( +143 h ( M&& h & A(W& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +89 h ' A(.& h & .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' M&& h & A(\\& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&(, +7 h , .t\Elbow.reconfigure TightElbow A(p- M&$&.,(\ #b h -+ .t\SE.seek 20.04 Pucks below Top Plate A()' +27 h ' M&u% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&<& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &8' +22 h ' .t\SE.seek 21.04 Pucks below Top Plate A(' +36 h ' M&e& h & M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'Q( +416 h ( A(l& h & A(& h & M&& h &ӷ M&'% h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &O( +142 h ( M&@& h & A(c& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&k, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &n1 +150 h 1 M&E% h %Y A(_& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&A'υ +21 h 'υ M&M& h & A(6& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' A(o% h % ."downElevator"\SE.seek :down A('X +53 h 'X ."downElevator"\SE.coast A(5 +47 (]% h %X M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'D @ +19 h @ .t\CS.seek 10ml C'q' +7 h ' .t\CSV.dial between air and CTV C'3 ` +18 h ` .t\AIV.dial :bypass C'1 { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'G& h & .tHand.open M&U1 +2 h 1 M&& h & M&-% h %\  .t\Elbow.seek FlushPuck::Garage M&\ (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&+% h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +265 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'Z& h &W .tHand.open M&V1 +150 h 1 M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%; +151 h P%,% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%x' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%J  +37 h  .t\PS.seek 0ml P%m' +1597 h ' .t\PSV.dial PSR P%z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%C' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%5  C'  h +75 .t\PSV.dial :mixloop P%I +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%4  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%N' +2609 h ' .t\PSV.dial :mixloop P%` +38 h .t\PS.seek 0ml P%a' +2610 h ' .t\PSV.dial :air P%h  +37 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :mixloop P%P +38 h .t\PS.seek 0ml P% ' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&d& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&r' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C'T( +416 h ( M&& h &ӷ M&{% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &X(- +264 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&v% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'i& h & M&s% h %\  A(|% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (w(~ +358 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b ("& h &~ M&&& h &- M&^% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&W& h & A(.& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +89 h 'н A(\\& h &~ .t\SE.seek 20.71 Pucks below Top Plate A(#' +57 h ' A(p& h &~ M&u& h & .t\Elbow.seek 3 M&( +7 h ( M&<& h & A(L& h &~ .t\Forearm.seek 3 + 300 counts M&e'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&:& A(~'X h & M&9% h %- .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C')(W +416 h (W .tHand.open M&81 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'f  +18 h  .t\CS.seek 5ml C'l'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%'  +38 h  .t\PS.seek 2.5ml P%@' +2609 h ' .t\PSV.dial :mixloop P%c +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%n  +151 h  .t\PS.seek :empty P%E' +2610 h ' .t\CBV.dial :waste1 C'_ @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'M  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'6 @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'o  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'S ` +19 h ` .t\CSV.dial :air C'O @ +38 h @ .t\CS.seek 10ml C']' +2647 h ' .t\CSV.dial CTV C'!  +37 h  .t\CS.seek 0ml C'D'4 +2648 h '4 .t\CBV.dial :waste1 C'3 @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'1  +151 h C'G& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&U% h %\  A(% h %X A(-. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &  M&& h &ӷ ."raiseStack"\SE.seek :upperLimit A('Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'k( +23 h 'Z +393 ( A(& h &  A(& h &  M&+& h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &B( +142 h ( M&j& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &*' +93 h ' .t\Elbow.hold M&Q, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Z1 +150 h 1 M&V% h % A(/& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&;'; +20 h '; M&,& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +23 h ' A(x% h %Z ."downElevator"\SE.seek :down A(z'X +100 (m% h % M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%I  +2 A(6 +111 h  P%4% h % .t\PS.seek 0.499ml P%C' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%`  +38 h .t\CBV.dial AIV C'N  +37 h .t\PRV.dial between CBV and air P%h  +19 h  .t\PRV.dial :air P%a  +19 h  P%P% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %b' +250 h ' .t\PRV.dial :waste2 P%d @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %=' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'}& h & .tHand.open M&[1 +2 h 1 M&R& h & M&Y% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&F/ +150 h / M&>& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'|(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  C'^& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%W @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%.  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%\\  +75 h  .tdelay 2 seconds +200 P%X% h % .t\PS.seek 1.499ml P%p' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%u' +1094 h ' .t\PSV.dial :air P%<  +37 h  .t\PS.seek 0ml P%L' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%K +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%2  +37 h  .tdelay 2 seconds +200 P%:% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %9'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%y  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%e'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%#  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%~  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%T @ +75 h @ .t\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial :air P%(  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%f +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%H' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%w  +75 h  .t\PS.seek 2.5ml P%q' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%l +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%'% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb % ' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'c & +50 h & .t\AWV.select :waste C' +6 P%g% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %%'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'n { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %@' +1313 h ' .t\PSV.dial :air P%_  +38 h  .t\PS.seek 2.5ml P%M' +2610 h ' .t\PSV.dial :PML P%6 +37 h .t\PS.seek 0ml P%o' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'S& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'O& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T .t\AWV.select :air F*!  .t\AIV.dial :bypass .\PSV.dial PSR P%] +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*G I +129 h I F*U% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%-  +37 h  .t\PS.seek 0ml P%A' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%? +21 h  F*)% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%+' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*t  +22 h  F*Z% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *V' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%/ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%,' +217 ."MFBPCR"\ATV.dial :AIV F*$ I +43 h I F*x% h % ."MFBPCR"\AS.seek 0.469ml F* ' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%B +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%m' +2609 h ' .t\PSV.dial PSR P%5 +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%;' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%I' +739 h ' ."MFBPCR"delay 5 seconds +500 F*49 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*J n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*z% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%E +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%`' +2610 h ' .t\PSV.dial PSR P%N +113 h P%h% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%a' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%P  +37 h  .t\PS.seek 0ml P%C' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%d +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%b' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%[ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %R'k +2678 h 'k .t\PSV.dial :waste1 P%Y  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*{ I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *Q'V +849 h ' .t\PSV.dial :air P%F  +113 h  .t\PS.seek 2.5ml P%>' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*i  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*k I +42 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %^' +618 h ' ."MFBPCR"\ATV.dial :waste F*W n +129 h n ."MFBPCR"\AS.seek :empty F*s'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*.  +86 h  F*\\% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *X' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*=  +43 h F*p% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *<'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'u  +37 h  C'K% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'2'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C':' +1625 h ' ."connect"\CSV.dial CTV C'L  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'9'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*y  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*#'ݏ +110 h '4 .t\CSV.dial CSR C'~  +37 h  C'e% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*T  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*('& +324 h '& ."MFBPCR"delay 3 seconds +98 h 'P .tdelay 5 seconds +202 ."MFBPCR"\ATV.dial ASV F*8 n +107 h n F*f% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'w' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'l'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&'& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +7 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M& ' +90 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'%( +416 h ( M&_& h &ӷ M&@% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +265 h (- M&S& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +91 h 'η .tHand.open M&]1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'G& h & M&U% h %\  A(-% h %X A(7. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (1(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&A& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&& h & A()& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &?'н +89 h 'н A(& h &~ ."WCR"\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A( & h &~ M& & h & ."WCR"\Elbow.seek 3 M&( +7 h ( M&\ & h & A(t& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&Z'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down M&j& A(/'X h & M&$% h %- ."WCR"\Forearm.seek :retract M&x'\ +38 h '&0 ."MFBPCR"delay 3 seconds +28 h 'X ."downElevator"\SE.coast A(5 +25 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&V& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' ."WCR"\CC.seek :closed C'5(W +14 ."MFBPCR"\ATV.dial :waste2 F** % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +359 h (W ."WCR"Hand.open M&1 +150 h 1 M&;% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +51 h 'R ."MFBPCR"\ATV.dial :air2 F*J  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *z' +18 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +7 h + S)E% h % C'I& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +304 h ' ."WCR"\Exhaust.select :close S) +6 #"WCR"\Sampling 1500.0ml #\Sampling only 25ml to speed simulation .Intake.select :close .\Exhaust.select :close .\SS.reconfigure SSconfig .\SS.hold S)#.0X'V +3473 h 'V ."MFBPCR"\SPE.coast F*r  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2709h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*"  +10 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*X  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*u' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*2  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*D' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*<'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*8' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 F*(  +65 h ."MFBPCR"\ARV.dial :air1 F*f  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F*w'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254531 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'c  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'v @ +76 h @ +50 S)g% h % ."WCR"\CBV.dial between CSV and waste2b C'|  +94 h  C'_% h % ."WCR"\CSV.dial between air and CTV C'& ` +19 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'S  ."WCR"\CSV.dial CTV C'@  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'q'4 +1920 h '4 ."WCR"\CBV.dial :waste2b C']  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'O' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'6  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'3  +37 h  C'U% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'7' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'G @ +75 h @ +50 S)-% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'!% h % ."WCR"\CSV.dial between air and CTV C'A ` +57 h ` ."WCR"\CS.seek 7.288ml C')' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'M' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C't  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'o  +38 h  C'\ % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'+' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)?% h % ."WCR"\CBV.dial between CSV and waste2b C'$  +94 h  C'B% h % ."WCR"\CSV.dial between air and CTV C'Z ` +57 h ` ."WCR"\CS.seek 7.288ml C'V' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'/  '*  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '%' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'x& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&;% h %\  A(J% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (I( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&E& h &ӷ M&m% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &1( +142 h ( M&,& h & A(h& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &N' +90 h ' A( & h &  ."WCR"\SE.seek -0.29 Pucks below Top Plate A(P'\\ +264 h '\\ M&`& h & A(a& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M&C, +7 h , ."WCR"\Elbow.reconfigure TightElbow A([- M&$&.,(\ #b h -+\\ ."WCR"\SE.seek 0.04 Pucks below Top Plate A(Y' +27 h ' M&d% h % A(z& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&}'; +21 h '; M&{& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &F' +22 h ' ."WCR"\SE.seek 1.04 Pucks below Top Plate A(R' +36 h ' M&& h & M&i% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&Q(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&"/ +150 h / ."WCR"\CC.seek :open C'b( +416 h ( A(>& h &  A( & h &  M&& h &ӷ M&W% h % ."WCR"\Forearm.seek :retract M&.'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &k( +143 h ( M&=& h & A(p& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &\\' +92 h ' ."WCR"\Elbow.hold M&X, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &K1 +150 h 1 M&% h % A(2& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&L'; +21 h '; M&u& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &:' +22 h ' A(D% h % ."downElevator"\SE.seek :down A('X +100 (~% h %S M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'9& h & .tHand.open M&e1 +2 A(6 h 1 M&& h & M&T% h %\  .t\Elbow.seek FlushPuck::Garage M&<(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&f'η +91 h 'η .tHand.close M&H/ +150 h / M&8& h &- M&% h %η .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'|& h &W .tHand.open M&_1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'@& h &W C'S% h % .t\CSV.dial CSR C'  +38 h  C']% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'q'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '6% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C'U +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'O'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'7& h &W C'G% h %4 .t\CSV.dial :flush C'-  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'A% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C') @ +75 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C't% h %4 .t\CSV.dial :flush C'o  +76 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +38 h @ C'+% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV C'?  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'$  +75 h  .t\CS.seek 0ml C'B'4 +766 h '4 #t\Flushing upper manifold C'Z& h &W C'j% h %4 .t\CSV.dial :flush C'*  +76 h  .t\CS.seek 5ml C'/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'x  +37 h +75 .t\CTV.dial :air C';  +151 h .t\CS.seek 0ml C'J'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'E'4 +507 h '4 C'm& h &W C',% h %4 .t\CSV.dial :flush C'h  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'`'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'a'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +506 h '0 .t\CSV.dial :waste C'd +76 h .t\CS.seek 0ml C'z'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'{& h &W C'% h %4 .t\CSV.dial CSR C'  +151 h  C'i% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'N'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'1' +415 h ' ."connect"\CSV.dial CBV C'P .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'r  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'Y  +113 h  C'F% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'C'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'5  '  +38 h  +113  .t\CBV.dial :waste1 C'> @ +38 h @ .t\CS.seek 0ml C' '4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '='P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'p' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'Q  +75 h  .t\CS.seek 0ml C'K'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'u'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'. 'D  +38 h +75 .t\CTV.dial :air C'k  +151 h .t\CS.seek 0ml C'~'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'9 +37 h .t\CS.seek 0ml C'e'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'T% h %4 .t\CSV.dial :flush C'\\  +38 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C':% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +507 h '0 .t\CSV.dial :waste C'X +38 h .t\CS.seek 0ml C'H'4 +506 h '4 C'8& h &W C'% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'f'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'l% h %0 .t\CS.seek 5ml C'<'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +507 h '0 .t\CSV.dial :waste C'| +75 h .t\CS.seek 0ml C'_'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'&& h &W C'@% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'v% h %p .t\CS.seek 7ml C'%'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'6  'q  +38 h  +113  .t\CBV.dial :waste1 C'U @ +37 h @ .t\CS.seek 0ml C'3'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'7& h &W C'G% h %4 .t\CSV.dial :flush C'-  +76 h  .t\CS.seek 5ml C'O'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'A% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C')'4 +765 h '4 #tDisinfecting external Intake with kill C'& h &W C' % h %4 .t\CSV.dial CSR C't  +38 h  C'o% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C' 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'M' +416 h ' ."connect"\CSV.dial CBV C'+ .t\CBV.dial CSV C'\  +37 h +76 .t\CTV.dial :intake C'?  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'$' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +606 h '4 .t\CSV.dial CSR C'j  +113 h  C'*% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'B'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'V' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'/'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&J' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&m& h &ӷ M&,% h % .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &E(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&`1 +150 h 1 M&a% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'[& h & .tHand.open M&{1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&P/ +150 h / M&& h &- M&1% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'5& h &W .tHand.open M&1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'W% h %4 .t\CSV.dial CSR C'  +113 h  C'=% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'Q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'p'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '2'4 +2010 h '4 #t\Purging external Intake C'K& h &W C'u% h %4 .t\CSV.dial :flush C'D  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'k .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'~'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'9  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'T  +76 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +38 h @ C'R% h %p .t\CS.seek 7ml C':'i +416 h 'i ."connect"\CSV.dial CBV C'L .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'('4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'#'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'X'4 +506 h '4 C'I& h &W C'8% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'y'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C'% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'f'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'' +75 h .t\CS.seek 0ml C'|'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&b& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &&(ӷ +6 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'v( +416 h ( M& & h &ӷ M&6% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &U(- +264 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&-1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:48 #\Preparing to sleep until Sat Sep 29 06:01:48 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST29-Sep-18 #\Continuing at 06:01CEST29-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:29:53CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST29-Sep-18" +235125 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2709h25ml.pcr F*A% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b **'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*c% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*Y  +10 h  F*F% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*>  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*=  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*}% h %Fj ."MFBPCR"\AS.seek 0.524ml F*/' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*Q  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*p  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +107 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*9'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*T' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*e  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2711h25ml.pcr F*(% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*#  +22 h ."MFBPCR"\ATV.dial :air3 F*I % +86 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +21 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*<% h %n ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*]% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*7  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*-% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*Z% h %V ."MFBPCR"\AS.seek 0.5ml F*B'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*c  +22 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*J' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*`  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.536ml F*h'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +108 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*{'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.6ml F*z'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*E  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*N% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Y'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2713h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*r  +21 h ."MFBPCR"\ATV.dial :air3 F*5 % +87 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*D% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*9  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*I% h %m ."MFBPCR"\AS.seek 0.82ml F*8'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ARV.dial :carrier F*b  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*7  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *%'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*q n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*3' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*A I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*$% h % \\ ."MFBPCR"\AS.seek 0.326ml F*M'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2716h25ml.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*V  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*P  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*F'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*5% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +10 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F*u'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*9  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*T n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*L% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*:' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*#  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*\\'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*X'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*'' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*<  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*]'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2718h25ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*C % +86 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *7'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*-% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*%'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*w% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*\ % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +21 h F*?% h % ."MFBPCR"\AS.seek 0.81ml F**'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*M'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*['(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +21 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*N I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*d % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*F' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*r  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*Y'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*>% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*u  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *9' +424 h ' ."MFBPCR"\ATV.dial :ASV F*~ n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*T'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*L n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *I' +424 h ' ."MFBPCR"\ATV.dial :air3 F*:% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.71ml F*H' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*X% h % H ."MFBPCR"\AS.seek 0.735ml F*l' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*y% h % Ű ."MFBPCR"\AS.seek 0.72ml F*<' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*f n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*@% h % zG ."MFBPCR"\AS.seek 0.365ml F*'' +7161 h ' ."MFBPCR"\ATV.dial :waste F*b n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*6% h % ."MFBPCR"\AS.seek 0.579ml F*"' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %  ."MFBPCR"\AS.seek 0.604ml F*7'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +21 h F*G% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*_' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*A n +86 h n ."MFBPCR"\ASV.dial :wasteR F*w  +22 h  ."MFBPCR"\AS.seek 0ml F*%'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*3% h %V ."MFBPCR"\AS.seek 0.01ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +21 h % ."MFBPCR"delay 3 seconds +300 F*t% h % q ."MFBPCR"\AS.seek 0.605ml F*+'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\ % h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*o' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*?  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *j'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F**  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *V' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *M'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*B n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +202 h 'R ."MFBPCR"\SPE.coast F*c  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*;'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*m  +43 h  F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*`  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*a  +65 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *['Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*h% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *i' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*P I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*{  +42 h  F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*d% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*E n +65 h n F*r% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *5' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*F I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*>  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Y': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*W n +150 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*=  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*} % +43 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*p'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %0 ."MFBPCR"\AS.seek 0.985ml F*D' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*k% h % ."MFBPCR"\AS.seek 0.97ml F*.'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*~ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *#' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *(' +9988 h ' ."MFBPCR"\ATV.dial AIV F*: I +43 h I ."MFBPCR"\AS.seek 0.02ml F*I' +9880 h ' ."MFBPCR"\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.seek 0ml F*\\'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*X  +21 h ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*@'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*''n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*C  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*6  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *&'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*w  +32 h  ."MFBPCR"\ATV.dial :air3 F*_ % +87 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V @CEST1538085624.45 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *V'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *M'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*x % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 ."shutdown"\Elbow.coast M&5 +2 &6 C'5 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538193708.11 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )m h  C' 'n h  P% %; h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ C' 2\ F*\ 9\ S)&\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *,% h %V .t\AS.configure ASconfig S)`% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:30:49CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #9) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'k& h & .tHand.open M&D1 +150 h 1 M&~& h &_ M&.% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &e(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &L'η +91 h 'η .tHand.close M&T/ +150 h / M&& h &- M&R% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +264 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'I& h &W .tHand.open M&\\1 +150 h 1 M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'l  +150 h  C'f% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '<% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'b'[ +463 h '[ .t\CSV.dial :waste C'@ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'S'4 +2009 h '4 #t\Purging external Intake C']& h &W C''% h %4 .t\CSV.dial :flush C'C  +38 h  .t\CS.reconfigure CSfast C'#d @b .t\CS.seek 10ml C'6' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +75 .t\CTV.dial :intake C'&  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'-'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'"  +75 h #t\Flushing upper manifold C'q& h &W C'G% h %4 .t\CSV.dial :flush C'7  +75 h  .t\CS.seek 5ml C'w'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +37 h @ C'%% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'3'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'O +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C')% h %4 .t\CSV.dial :flush C't  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'\ 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'+'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'$'0 +506 h '0 .t\CSV.dial :waste C'? +76 h .t\CS.seek 0ml C'o'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +7 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'c( +416 h ( M&B& h &ӷ M&x% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &U(- +264 h (- M&|& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &^'η +91 h 'η .tHand.open M&1 +150 h 1 M&!% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:30:54CEST30-Aug-18\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:30:49CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*0% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&m% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(n% h %X A(;. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*, % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *`' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&g& h &- M&a% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &4( +166 h ( M&J& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &h'н +90 h 'н A([& h &~ .t\SE.seek 18.71 Pucks below Top Plate A(P' +76 h ' A(i& h &~ M&z& h & .t\Elbow.seek 3 M&N( +7 h ( M&{& h & A( & h &~ .t\Forearm.seek 3 + 300 counts M&1'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down A(r'X M&d& h & M&% h %- .t\Forearm.seek :retract M&5'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&W(ӷ +143 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'2(W +416 h (W .tHand.open M&u1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +90 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)}+ +7 h + S) % h % C'k& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +281 h ' .t\SS.coast S)5 #tLysing: Adding 1.5ml lysis .\CBV.dial between CSV and waste1,avoiding: :DBV C'K  +169 h .t\CTV.dial CSV C'P  +151 h  .t\CSV.dial CSR C'1  +75 h  C'r% h %4 #t\Pulling 1ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1ml C'#P d@b 'N' +350 h ' .tdelay 5 seconds +500 .\CSR.select :air C' +6 '5% h % .t\CS.seek 6ml C'c' +1373 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'u +150 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b '' +710 h ' .t\CSV.dial CSR C'  +150 h  C'k% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'Q' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %  .t\CS.seek 4.5ml C''` +859 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'}  +38 h  .t\CS.seek 1.275ml C'~' +919 h ' #tHeating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'.   'F9 h 9d +100 C'9 h 9d +100 C'99 h 91d +100 C'D9 h 9J d +100 C'`9 h 9bd +100 C'9 h 9zd +100 C'R9 h 9d +100 C'9 h 9d +100 C'T9 h 9 d +100 C':9 h 9$d +100 C'(9 h 9(d +100 C'e9 h 9 ,d +100 C'/9 h 9$0d +100 C'p9 h 9<4d +100 C'I9 h 9S8d +100 C'\\9 h 9kd +100 C'G9 h 9Td +100 C'&9 h 9kd +100 C'79 h 9d +100 C'-9 h 9d +100 C'%9 h 9d +100 C'w9 h 9d +100 C'9 h 9d +100 C'A9 h 9d +100 C'39 h 9 d +100 C'S9 h 9 d +100 C'q9 h 96d +100 C'9 h 9Ld +100 C'9 h 9bd +100 C')9 h 9xd +100 C'_9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C't9 h 9d +100 C' 9 h 9d +100 C'+9 h 9d +100 C'?9 h 9(d +100 C'9 h 9>d +100 C'\ 9 h 9Td +100 C'j9 h 9id +100 C'O9 h 9d +100 C'*9 h 9d +100 C'$9 h 9d +100 C'V9 h 9d +100 C'H9 h 9d +100 C'9 h 9d +100 C'x9 h 9  d +100 C'|9 h 9 d +100 C'#9 h 9 *d +100 C'9 h 9 ?d +100 C's9 h 9 Td +100 C'9 h 9 j d +100 C'B9 h 9 $d +100 C'Z9 h 9 (d +100 C'M9 h 9 ,d +100 C'09 h 9 0d +100 C'm9 h 9 4d +100 C'n9 h 9 8d +100 C';9 h 9 9 h 9 d +100 C'P9 h 9 d +100 C'19 h 9 1d +100 C'r9 h 9 Dd +100 C'59 h 9 Xd +100 C'N9 h 9 kd +100 C'u9 h 9 ~d +100 C'c9 h 9 d +100 C' 9 h 9 d +100 C'k9 h 9 d .t\CH.hold 5:00,85C C'   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'Q'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'} @ +113 h @ .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 5ml C'F'p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'9 @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'D  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'` @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'R +113 h .t\CBV.dial CSV C'  +18 h C'T% h %4 .t\CS.seek 5ml C':'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'(  +37 h C'e% h %p .t\CS.seek 7ml C'/'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'p @ +38 h @ .t\CS.seek 10ml C'I' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'\\ ` +132 h ` #tLysing Complete C'X& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&% h %\  A(% h %X A(f. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (8( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&<& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &l( +143 h ( M&@& h & A(b& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &]' +89 h ' A('& h &  .t\SE.seek 0.71 Pucks below Top Plate A(C'K +254 h 'K M&y& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b & ' +25 h ' .t\Elbow.hold M&", +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(6- h -+K .t\SE.seek 1.04 Pucks below Top Plate A(o' +27 h ' M&G% h % A(&& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&7'; +21 h '; M&-& h & A(%& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &w' +22 h ' .t\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M&A& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +89 h '\  .t\Elbow.seek CC M&q(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h &  A(& h &  M&t& h &ӷ M& % h % .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &?( +142 h ( M&& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &j' +93 h ' .t\Elbow.hold M&O, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &*1 +150 h 1 M&$% h % A(V& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &H'; +20 h '; M&& h & A(x& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &|' +23 h ' A(#% h % ."downElevator"\SE.seek :down A('X +100 (s% h %B M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'Z @ + A(6 +17 h @ .t\CS.seek 10ml C'M' +7 h ' .t\CSV.dial between air and CTV C'0 ` +19 h ` .t\AIV.dial :bypass C'm { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&;1 +2 h 1 M&& h & M&,% h %\  .t\Elbow.seek FlushPuck::Garage M&!(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&/ +150 h / M&J& h &- M&% h %η .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +264 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &{' +91 h ' .t\CC.seek :closed C'v(W +416 h (W C'i& h &W .tHand.open M&d1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%4' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%h  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%K +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%>' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'1  P%P  h +76 .t\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%5  h .t\PS.seek 0ml P%N' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%c' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%k' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%~ +38 h .t\PS.seek 0ml P%}' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.seek CC M&D' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'F( +416 h ( M&R& h &ӷ M&% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &:'η +91 h 'η .tHand.open M&p1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'\\& h & M&X% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (f(~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&<& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &@( +166 h ( M&b& h & A('& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &W'н +89 h 'н A(Y& h &~ .t\SE.seek 17.71 Pucks below Top Plate A(y'* +86 h '* A(& h &~ M&6& h & .t\Elbow.seek 3 M&G( +7 h ( M&&& h & A(-& h &~ .t\Forearm.seek 3 + 300 counts M&%'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +150 h / ."downElevator"\SE.seek :down M&3& A(]'X h & M&l% h %- .t\Forearm.seek :retract M&C'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' .t\CC.seek :closed C'o(W +416 h (W .tHand.open M&w1 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +151 h  .t\CSV.dial CTV C'2  +19 h  .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C't'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%\ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%)  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%q +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%*  +151 h  .t\PS.seek :empty P%$' +2610 h ' .t\CBV.dial :waste1 C'V @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'#  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'+ ` +19 h ` .t\CSV.dial :air C'? @ +38 h @ .t\CS.seek 10ml C's' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'j'4 +2647 h '4 .t\CBV.dial :waste1 C'Z @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'H  +150 h C'0& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&m% h %\  A(n% h %X A(;. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (,& h &  M&& h &ӷ M&|% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &g( +143 h ( M&a& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +89 h ' A(I& h &  .t\SE.seek 1.71 Pucks below Top Plate A(O': +244 h ': M&& h & A(J& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&B, +6 h , .t\Elbow.reconfigure TightElbow A(^- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(U' +27 h ' M&z% h % A(!& h &  .t\Forearm.force -20 .\Forearm.seek 2 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &['; +20 h '; M&& h & A(i& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &d' +23 h ' .t\SE.seek 3.04 Pucks below Top Plate A(' +36 h ' M& & h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +89 h '\  .t\Elbow.seek CC M&h(ӷ +143 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&P/ +150 h / .t\CC.seek :open C'1( +416 h ( A(r& h &  A(5& h &  M&>& h &ӷ M&u% h % .t\Forearm.seek :retract M&N'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +143 h ( M&c& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &k' +92 h ' .t\Elbow.hold M&~, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&9'; +20 h '; M&`& h & A(=& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &E' +22 h ' A(v% h % ."downElevator"\SE.seek :down A(R'X +100 (% h %1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%e  +2 A(6 +111 h  P%Q% h % .t\PS.seek 0.499ml P%p' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +37 h .t\PRV.dial :air P%T  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%\\  +38 h .t\CBV.dial AIV C'X  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %<' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %:' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'b& h & .tHand.open M&'1 +2 h 1 M&Y& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &- .t\Forearm.seek :garage M&-'η +91 h 'η .tHand.close M&// +150 h / M&A& h &- M&3% h %η .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +264 h (ӷ M&W& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'y(W +416 h (W C'%& h &W .tHand.open M&]1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  C'F& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%w @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%"  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%2% h % .t\PS.seek 1.499ml P%7' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%f' +1094 h ' .t\PSV.dial :air P%M  +38 h  .t\PS.seek 0ml P% ' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%}% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %\ 'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%)  +75 h .tdelay 2 seconds +200 P%q% h %r .t\PS.seek 0.475ml P%*' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%V'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %#' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%+ +76 h .t\PTV.dial :PRV,avoiding: :puck P%? @ +75 h @ .t\PS.seek 0ml P%t' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%s' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Z +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%H' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%0  +76 h  .t\PS.seek 2.5ml P%m' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%n +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%;  +75 h P%,% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'| & +50 h & .t\AWV.select :waste C' +6 P%a% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'I { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb % ' +1313 h ' .t\PSV.dial :air P%_  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P%J +38 h .t\PS.seek 0ml P%^' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'z& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'!& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*i  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%8' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*g I +129 h I F*O% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +65 .t\PSV.dial :waste1 P%4  +38 h  .t\PS.seek 0ml P%K' +1597 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%U' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%B' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*P  +22 h ' .t\PSV.dial PSR P%{ +20 h  F*o% h %6 ."MFBPCR"\AS.seek 0.329ml F*r'ͯ +93 h P%5% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%>' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*u % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*h  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%`' +217 ."MFBPCR"\ATV.dial :AIV F*= I +43 h I F*v% h % ."MFBPCR"\AS.seek 0.469ml F*N' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%D +113 h P%k% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%9' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%e' +740 h ' ."MFBPCR"delay 5 seconds +500 F*Q9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*E n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*R% h % ."MFBPCR"\AS.seek 0.041ml F*'% +337 h ' .t\PSV.dial PSR P%$ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%.' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%T +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%\\' +2610 h ' .t\PSV.dial PSR P%X +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%p' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%<' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%' +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %Y'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %6' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*& I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *~'V +850 h ' .t\PSV.dial :air P%/  +113 h  .t\PS.seek 2.5ml P%A' +2609 h ' .t\PSV.dial :mixloop P%3 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %(' +5107 h ' .t\PSV.dial :air P%-  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %W' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *l'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*1 I +43 h I F*%% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +171 h ' .t\PSV.dial :mixloop P%G +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %F' +618 h ' ."MFBPCR"\ATV.dial :waste F*w n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*"  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *2' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*:  +43 h F*7% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *M'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'f  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'}' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  ')  +37 h  +113  .t\CBV.dial :waste1 C'q @ +38 h @ .t\CS.seek 0ml C'\ '4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F**% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'V% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'+'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*?  +21 h F*#% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*t n +108 h n F*Z% h %& ."MFBPCR"\AS.seek 0.092ml F*s'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'0' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'n'4 +2647 h '4 C';& h &W #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&I/ +150 h / .t\CC.seek :open C'( +416 h ( M&_& h &ӷ M& % h % .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &i'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'g& h & M&O% h %\  A(4% h %X A(L. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (8(~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ([& h &~ M&K& h &- M&d% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &U( +166 h ( M&P& h & A(o& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &{'н +89 h 'н A(5& h &~ ."WCR"\SE.seek 16.71 Pucks below Top Plate A('; +96 h '; A(B& h &~ M&u& h & ."WCR"\Elbow.seek 3 M&r( +6 h ( M&& h & A(h& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &>/ +150 h / ."downElevator"\SE.seek :down M&c& h & A('X M&=% h %- ."WCR"\Forearm.seek :retract M&v'\ +9 h '&0 ."MFBPCR"delay 3 seconds +82 h '\  ."WCR"\Elbow.seek CC M&(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +75 ."MFBPCR"\ATV.dial :waste2 F*D % +16 h ' ."WCR"\CC.seek :closed C'k(W +27 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +389 h (W ."WCR"Hand.open M&1 +150 h 1 M&9% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +21 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *R' +49 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)N+ +6 h + S)$% h % C'e& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'T  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *='V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*U % +107 h % F*{% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*D  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*r I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*v' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*s% h % ."MFBPCR"\AS.seek 0.13ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +21 h F*E% h % ."MFBPCR"\AS.seek 0.23ml F*$' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +21 h % ."MFBPCR"delay 3 seconds +300 F*T% h % ."MFBPCR"\AS.seek 0.255ml F*`'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*8% h %X ."MFBPCR"\AS.seek 0.24ml F*N' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*. @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*''V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*R'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *&'\  +1031 h '\  F*b% h %\  ."MFBPCR"\AS.seek 0.5ml F*/'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*6% h %(A ."MFBPCR"\AS.seek 0.6ml F*3'\  +1031 h '\  F*Y% h %\  ."MFBPCR"\AS.seek 0.5ml F*('(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*~ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +3472 h 'V ."MFBPCR"\SPE.coast F*-  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2909h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*%  +64 h  ."MFBPCR"\ARV.dial :carrier F*G  +11 h  F*<% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*:  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *l'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*2  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*j' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*q % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +107 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*V'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*#  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*Z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F*0'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254561 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'|  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'; @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'@ @ +75 h @ +50 S)a% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'_% h % ."WCR"\CSV.dial between air and CTV C'C ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'z  '  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'm'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '!' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'J  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'O% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'L' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'g @ +75 h @ +50 S)4% h % ."WCR"\CBV.dial between CSV and waste2b C'd  +95 h  C'i% h % ."WCR"\CSV.dial between air and CTV C'K ` +56 h ` ."WCR"\CS.seek 7.288ml C'o' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'5  ."WCR"\CSV.dial CTV C'P  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'B'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'u  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'h  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'^  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'>' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'U @ +76 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'=  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'D  'r  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C's  +151 h C'v& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&9% h %\  A(E% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (e( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (T& h &  M&$& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &8( +143 h ( M&`& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(N& h &  ."WCR"\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') M&\\& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M&p, +7 h , ."WCR"\Elbow.reconfigure TightElbow A('- M&$&.,(\ #b h -+) ."WCR"\SE.seek 3.04 Pucks below Top Plate A(' +26 h ' M&% h % A(R& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &b'; +21 h '; M&&& h & A(6& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &/' +22 h ' ."WCR"\SE.seek 4.04 Pucks below Top Plate A(Y' +36 h ' M&3& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +89 h '\  ."WCR"\Elbow.seek CC M&~(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ ."WCR"\Forearm.seek CC M&%' +91 h ' ."WCR"Hand.close M&G/ +150 h / ."WCR"\CC.seek :open C'<( +416 h ( A(A& h &  A(W& h &  M&]& h &ӷ M&w% h % ."WCR"\Forearm.seek :retract M&"'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &1( +142 h ( M&:& h & A(7& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &S' +93 h ' ."WCR"\Elbow.hold M&2, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&l% h % A( & h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M& '; +21 h '; M&f& h & A(q& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +22 h ' A(j% h % ."downElevator"\SE.seek :down A(*'X +100 (x% h %  M&)% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&V1 +2 A(6 h 1 M&#& h & M&?% h %\  .t\Elbow.seek FlushPuck::Garage M&M(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&Z'η +91 h 'η .tHand.close M&H/ +150 h / M&t& h &- M&+% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +265 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &@' +90 h ' .t\CC.seek :closed C'a(W +416 h (W C'y& h &W .tHand.open M&_1 +150 h 1 M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'z& h &W C' % h % .t\CSV.dial CSR C'  +38 h  C' % h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'm'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'J% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'O +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '!'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'L& h &W C'g% h %4 .t\CSV.dial :flush C'4  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'd'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'K% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'P  '5  +37 h  +113  .t\CBV.dial :waste1 C'o @ +75 h @ .t\CS.seek 0ml C'u'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'B& h &W C'h% h %4 .t\CSV.dial :flush C'^  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'>% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +37 h  .t\CBV.dial :waste2b C'=  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing upper manifold C'& h &W C'c% h %4 .t\CSV.dial :flush C'D  +76 h  .t\CS.seek 5ml C'r'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C's .t\CBV.dial CSV C'v  +37 h +76 .t\CTV.dial :air C'9  +150 h .t\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'T +37 h .t\CS.seek 0ml C'$'4 +507 h '4 C'& h &W C'`% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'N'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'Q% h %0 .t\CS.seek 5ml C'\\'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'R'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'&& h &W C'6% h %4 .t\CSV.dial CSR C'3  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'X'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'8' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +112 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'-  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'/% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'p'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'G' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'(  'k  +37 h  +113  .t\CBV.dial :waste1 C'A @ +38 h @ .t\CS.seek 0ml C'W'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C']  +38 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ':'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'%  +38 h  .t\CBV.dial :waste2b C'~  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'l  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'f'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'j  '" +38 h +75 .t\CTV.dial :air C'1  +151 h .t\CS.seek 0ml C'x'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C')'0 +507 h '0 .t\CSV.dial :waste C'\ +37 h .t\CS.seek 0ml C'V'4 +507 h '4 #t\Flushing upper manifold C'#& h &W C'?% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Y @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} +112 h .t\CTV.dial :air .\CS.seek 0ml C'*'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'e'0 +507 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'H'4 +506 h '4 C't& h &W C'+% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'Z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'n% h %0 .t\CS.seek 5ml C'M'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C','4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C'_'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'C& h &W C'z% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'@% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'J  'm  +38 h  +113  .t\CBV.dial :waste1 C'O @ +38 h @ .t\CS.seek 0ml C''4 +765 h '4 #t\Flushing lower manifold's waste2b port C'L& h &W C'g% h %4 .t\CSV.dial :flush C'4  +76 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'K% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'P  +37 h  .t\CBV.dial :waste2b C'5  +76 h  .t\CS.seek 0ml C'o'4 +766 h '4 #tDisinfecting external Intake with kill C'[& h &W C'B% h %4 .t\CSV.dial CSR C'h  +37 h  C'^% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'u'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'> '  +37 h +76 .t\CTV.dial :intake C'{  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'=' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 .t\CSV.dial CSR C'c  +113 h  C'D% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'r'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&v& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +6 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&E' +91 h ' .tHand.close M&T/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&`% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &$(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &N'η +91 h 'η .tHand.open M&\\1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C''& h & .tHand.open M&&1 +2 h 1 M&6& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.seek :garage M&X'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&8% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &b' +91 h ' .t\CC.seek :closed C'p(W +416 h (W C'(& h &W .tHand.open M&k1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C'w% h %4 .t\CSV.dial CSR C'W  +113 h  C':% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'%'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '~% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '7'[ +462 h '[ .t\CSV.dial :waste C'l +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Purging external Intake C'& h &W C'f% h %4 .t\CSV.dial :flush C'j  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'1 +75 h .t\CTV.dial :intake C'q  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'x'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'\  +75 h #t\Flushing upper manifold C')& h &W C'#% h %4 .t\CSV.dial :flush C'?  +76 h  .t\CS.seek 5ml C'S'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'Y% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'* +38 h .t\CS.seek 0ml C'2'4 +506 h '4 C'e& h &W C't% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C';% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +507 h '0 .t\CSV.dial :waste C', +75 h .t\CS.seek 0ml C'y'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +6 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'@( +416 h ( M&& h &ӷ M&J% h % .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&41 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:03 #\Preparing to sleep until Mon Oct 01 06:02:03 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST01-Oct-18 #\Continuing at 06:02CEST01-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:32:20CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST01-Oct-18" +235154 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2909h25ml.pcr F*K% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*P  +22 h ."MFBPCR"\ATV.dial :air3 F*5 % +87 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*^'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +21 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*U  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*=  +65 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*T % +87 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *I' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*`'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +21 h F*\\% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *''R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2911h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*/% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +22 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*:  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*b% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*~  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*l' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*7  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*f'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*)  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*x  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2911h25ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *+'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*L  +32 h  ."MFBPCR"\ATV.dial :air3 F*C % +86 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*0% h %> ."MFBPCR"\AS.seek 0.835ml F*P'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*!'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2913h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*D  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*T  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*|  +21 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *a'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F*s' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*'% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*N  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*$  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*X% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2913h25ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*-  +22 h ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +21 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*l  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +31 h  ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F**  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2916h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*<  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*L  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*m n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*K I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*5 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*P'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*B  +107 h  ."MFBPCR"delay 7 seconds +700 F*!% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*[ n +21 h n ."MFBPCR"delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.6ml F*u'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*^  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*U  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2916h25ml.pcr F*c% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *T'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +21 h F*9% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*a'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*E'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +65 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2918h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*7  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*f'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*x  +22 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *#'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*? n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*.  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*S'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +22 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*n  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*M  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Z  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2918h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*p % +86 h % F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *L'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*0% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *B'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*>  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*{% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*v'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2920h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ARV.dial :carrier F*E  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*''(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *N'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*X I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*$' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.53ml F*/' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*-  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*k n +22 h n ."MFBPCR"delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*W  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*~  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2920h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*l @@T ."MFBPCR"\ASV.dial :SPEdelivery F*f  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*q  +151 h F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\ ' +423 h ' ."MFBPCR"\ATV.dial :ASV F*x n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*V  +43 h  F*)% h % ."MFBPCR"\AS.seek 0.62ml F*?'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *.'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*Y % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *e' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F**' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*S% h % ."MFBPCR"\AS.seek 0.71ml F*H' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h % H ."MFBPCR"\AS.seek 0.735ml F*n' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*M' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*Z n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*z% h % zG ."MFBPCR"\AS.seek 0.365ml F*,' +7161 h ' ."MFBPCR"\ATV.dial :waste F*< n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*J% h % ."MFBPCR"\AS.seek 0.579ml F*G' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %  ."MFBPCR"\AS.seek 0.604ml F*L'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*_' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +86 h n ."MFBPCR"\ASV.dial :wasteR F*0  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *d'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*P% h % ."MFBPCR"\AS.seek 0.58ml F*[' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h % q ."MFBPCR"\AS.seek 0.605ml F*>'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*^' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*= n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*{  +21 h  ."MFBPCR"\AS.seek 0ml F*U'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *c'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*D  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +202 h 'R ."MFBPCR"\SPE.coast F*|  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*T n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\\  +43 h  F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *E': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*Q% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *''Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*6 n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*N  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *3'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*$ n +65 h n F*-% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *(' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*/ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*A  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*k n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *]'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*w n +151 h n ."MFBPCR"\AS.seek 0ml F*W'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*:  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*b % +43 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h % ."MFBPCR"\AS.seek 0.96ml F*7'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %0 ."MFBPCR"\AS.seek 0.985ml F*j' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*1% h % ."MFBPCR"\AS.seek 0.97ml F*"'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*x I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *)'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *#'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*.  +43 h  F*Y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *}' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*e' +9880 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek 0ml F*S'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F**% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*2  +22 h ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*Z% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*,'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*p  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *C'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *5' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*B'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' @CEST1538258419.86 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*v % +108 h % ."MFBPCR"\AS.seek 0ml F*T'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 F*5 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast P%6 M&5 + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538366523.55 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' 'I h  P% %9 h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *`% h %V .t\AS.configure ASconfig S)\\% F*#,,'T(^20( b h % .t\SS.configure SSconfig C's& S)#.0X 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:33:17CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #10) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&j1 +150 h 1 M&x& h &_ M&"% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &V(- +166 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&?/ +150 h / M&.& h &- M&Y% h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'*(W +416 h (W C'e& h &W .tHand.open M&S1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C';& h &W C'+% h %4 .t\CSV.dial CSR C'n  +151 h  C'Z% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'M% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '<'[ +462 h '[ .t\CSV.dial :waste C'z +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Purging external Intake C' & h &W C',% h %4 .t\CSV.dial :flush C'p  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'J' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +75 .t\CTV.dial :intake C'C  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'4'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'G  +75 h #t\Flushing upper manifold C'm& h &W C'g% h %4 .t\CSV.dial :flush C'L  +76 h  .t\CS.seek 5ml C'0'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +507 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'P'4 +506 h '4 C'd& h &W C'o% h %4 .t\CSV.dial :flush C'h  +38 h  .t\CS.seek 3ml C'B'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'[% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'>'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +507 h '0 .t\CSV.dial :waste C'{ +75 h .t\CS.seek 0ml C'^'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'|( +416 h ( M& & h &ӷ M&v% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &F(- +264 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&y1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:33:22CEST30-Aug-18\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:33:17CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*@% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'l& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(I% h %X A(9. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +7 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*` % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *\\' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &~ M&a& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Q( +166 h ( M&E& h & A(6& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +89 h 'н A('& h &~ .t\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A(& h &~ M&R& h & .t\Elbow.seek 3 M&&( +6 h ( M&X& h & A(& h &~ .t\Forearm.seek 3 + 300 counts M&N'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &$/ +150 h / ."downElevator"\SE.seek :down A(-'X M&8& h & M&3% h %- .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.seek CC M&w(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&f1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)b+ +6 h + S)W% h % C'1& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +151 h  .t\CTV.dial :air,avoiding: :intake C'{  +150 h ."CSpush"\CSV.dial CTV .t\SS.seek 10ml S)=' +100 ."CSpush"\CSV.dial :air C'U @ +38 h @ ."CSpush"\CS.reconfigure CSfast .\CS.seek 0ml C'#d @b 'D'4 +6 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S)c +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'g9 h 9Td +100 C'C9 h 9kd +100 C'L9 h 9d +100 C'49 h 9d +100 C'9 h 9d +100 C'09 h 9d +100 C'9 h 9d +100 C'K9 h 9d +100 C'9 h 9 d +100 C' 9 h 9 d +100 C'm9 h 96d +100 C'59 h 9Ld +100 C'd9 h 9bd +100 C'o9 h 9xd +100 C'_9 h 9d +100 C'P9 h 9d +100 C'u9 h 9d +100 C'[9 h 9d +100 C'h9 h 9d +100 C'B9 h 9d +100 C'>9 h 9d +100 C'{9 h 9(d +100 C'U9 h 9>d +100 C'9 h 9Td +100 C'c9 h 9id +100 C'!9 h 9d +100 C'D9 h 9d +100 C'=9 h 9d +100 C'9 h 9d +100 C'H9 h 9d +100 C'*9 h 9d +100 C'v9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'O9 h 9 ?d +100 C'i9 h 9 Td +100 C'y9 h 9 j d +100 C' 9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'@9 h 9 0d +100 C'9 h 9 4d +100 C'I9 h 9 8d +100 C'99 h 9 '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &{( +143 h ( M&U& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &c' +92 h ' .t\Elbow.hold M&!, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &D1 +150 h 1 M&=% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &H'; +21 h '; M&*& h & A(v& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(O'X +100 (i% h % M&y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C'@ ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'I& h & .tHand.open M&91 +2 h 1 M&& h & M&`% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&l/ +150 h / M&E& h &- M&6% h %η .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +265 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'& h &W .tHand.open M&81 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Q' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%A' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%N  C'  h +75 .t\PSV.dial :mixloop P%& +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%(  h .t\PS.seek 0ml P%-' +2610 h ' .t\PSV.dial :air P%f  +38 h  .t\PS.seek 2.5ml P%|' +2609 h ' .t\PSV.dial :mixloop P%W +38 h .t\PS.seek 0ml P%1' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.seek 0ml P%b' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&k& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +7 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&\\' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'/( +416 h ( M&Y& h &ӷ M&%% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &.(- +264 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&q1 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'S& h & M&2% h %\  A(;% h %X A(#. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Z(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&M& h &- M&+% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &z( +166 h ( M&<& h & A(,& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &w'н +90 h 'н A(& h &~ .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(& h &~ M&J& h & .t\Elbow.seek 3 M&g( +6 h ( M&C& h & A(4& h &~ .t\Forearm.seek 3 + 300 counts M&'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &K/ +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&n% h %- .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C'^(W +416 h (W .tHand.open M&01 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'u'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'[' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'h'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%B  +37 h  .t\PS.seek 2.5ml P%U' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%o  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%m +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%D  +150 h  .t\PS.seek :empty P%=' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'*  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'v @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'> ` +18 h ` .t\CSV.dial :air C'{ @ +38 h @ .t\CS.seek 10ml C'i' +2647 h ' .t\CSV.dial CTV C'y  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'@ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'I& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&c% h %\  A(9% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (`( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (H& h &  M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &a( +142 h ( M&& h & A(t& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &O' +90 h ' A(e& h &  .t\SE.seek 4.71 Pucks below Top Plate A(!' +214 h ' M&l& h & A(E& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &6' +25 h ' .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b .t\SE.seek 5.04 Pucks below Top Plate A(F' +27 h ' M&R% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &''; +21 h '; M&P& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(s' +36 h ' M&& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'( +416 h ( A(&& h &  A((& h &  M&A& h &ӷ M&f% h % .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &W( +143 h ( M&|& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &1' +92 h ' .t\Elbow.hold M&", +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&k% h % A()& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &\ '; +21 h '; M&j& h & A(:& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(Y'X +100 (%% h % M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%V  +2 A(6 +111 h  P%~% h % .t\PS.seek 0.499ml P%q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%x +37 h .t\PRV.dial :air P%r  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%S  +38 h .t\CBV.dial AIV C'2  +37 h .t\PRV.dial between CBV and air P%;  +19 h  .t\PRV.dial :air P%#  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %M' +250 h ' .t\PRV.dial :waste2 P%+ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'<& h & .tHand.open M&,1 +2 h 1 M&& h & M&.% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&4'η +91 h 'η .tHand.close M&?/ +150 h / M&K& h &- M&% h %η .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &z(ӷ +264 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &d' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&g1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  C'/& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%0 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%G  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P% % h % .t\PS.seek 1.499ml P%L' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%Z' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%u' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%[ +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%B  +38 h  .tdelay 2 seconds +200 P%b% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%o  +75 h .tdelay 2 seconds +200 P%m% h %r .t\PS.seek 0.475ml P%D' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%U  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%*  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%v  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%> +76 h .t\PTV.dial :PRV,avoiding: :puck P%{ @ +75 h @ .t\PS.seek 0ml P%h' +2610 h ' .t\PSV.dial :air P%y  +75 h  .t\PS.seek 2.5ml P%i' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%@ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%c' +2609 h ' .tdelay 3 seconds +299 .\PSV.dial :PML P%9 +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %t'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'e { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%_  +38 h  .t\PS.seek 2.5ml P%l' +2609 h ' .t\PSV.dial :PML P%E +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'R& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& h &W ."MFBPCR"\ASV.dial :SPEdelivery F*P @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*a I +129 h I F*!% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *6'6 +65 .t\PSV.dial :waste1 P%Q  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%' +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%F' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%8 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*N  +22 h ' .t\PSV.dial PSR P%X +21 h  F*^% h %6 ."MFBPCR"\AS.seek 0.329ml F*&'ͯ +92 h P%(% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%A' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*f % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%]' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *k' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%) +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%j' +217 ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*T% h % ."MFBPCR"\AS.seek 0.469ml F*-' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%W +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%%' +2609 h ' .t\PSV.dial PSR P%\\ +113 h P%1% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%\ ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%s  +37 h  .t\PS.seek 0ml P%V' +740 h ' ."MFBPCR"delay 5 seconds +500 F*~9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*$ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Y% h % ."MFBPCR"\AS.seek 0.041ml F*`'% +336 h ' .t\PSV.dial PSR P%= +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%x' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial PSR P%2 +113 h P%;% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%#' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%+ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%M' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%.  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %J' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*C I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *"'V +850 h ' .t\PSV.dial :air P%?  +113 h  .t\PS.seek 2.5ml P%K' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %}' +5107 h ' .t\PSV.dial :air P%4  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %w' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*5  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *n'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*g' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %/' +618 h ' ."MFBPCR"\ATV.dial :waste F*0 n +129 h n ."MFBPCR"\AS.seek :empty F*d'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*G  +86 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*L% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'Z  +38 h  C'[% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'B'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'b' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'u  'o  +38 h  +112  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*U  +21 h ."MFBPCR"delay 3 seconds +300 F*D% h %_ ."MFBPCR"\AS.seek 0.434ml F**'ݏ +110 h '4 .t\CSV.dial CSR C'v  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'>'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*{  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*y'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*h n +108 h n F*@% h %& ."MFBPCR"\AS.seek 0.092ml F*i'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'I' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'9'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&H& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&e/ +150 h / .t\CC.seek :open C't( +416 h ( M&_& h &ӷ M&% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &l(- +264 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'a& h & M&!% h %\  A(Q% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (O(~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ('& h &~ M&& h &- M&8% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &F( +166 h ( M&N& h & A(^& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X'н +89 h 'н A((& h &~ ."WCR"\SE.seek 13.71 Pucks below Top Plate A(6'n +125 h 'n A( & h &~ M&f& h & ."WCR"\Elbow.seek 3 M&&( +7 h ( M&& h & A(& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +129 h '&0 ."MFBPCR"delay 3 seconds +21 h / ."downElevator"\SE.seek :down M&|& A()'X h & M&:% h %- ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.seek CC M&](ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&k& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &j' +46 ."MFBPCR"\ATV.dial :waste2 F*\\ % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +2 h ' ."WCR"\CC.seek :closed C'%(W +416 h (W ."WCR"Hand.open M&s1 +142 h 'R ."MFBPCR"\ATV.dial :air2 F*\  +8 h 1 M&~% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +13 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *Y' +78 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)-+ +7 h + S)=% h % C'V& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'r  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*I% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*H I +21 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*e % +22 h % F*z% h %H ."MFBPCR"\AS.seek 0.069ml F*p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*_% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*3'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*E I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *a' +4416 h ' ."MFBPCR"\ATV.dial :waste F*! n +128 h n ."MFBPCR"\AS.seek :empty F*Q'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*'% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*N' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %  ."MFBPCR"\AS.seek 0.604ml F*l'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*f% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *:'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*F % +107 h % F*X% h %V ."MFBPCR"\AS.seek 0.59ml F*k' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*W n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*6' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*& I +86 h I F*)% h % ."MFBPCR"\AS.seek 0.12ml F*T' +625 h ' ."MFBPCR"\ATV.dial :air3 F*i % +22 h % F*t% h % ."MFBPCR"\AS.seek 0.13ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*\ % h % ."MFBPCR"\AS.seek 0.23ml F*=' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +21 h % ."MFBPCR"delay 3 seconds +300 F*r% h % ."MFBPCR"\AS.seek 0.255ml F*j'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +21 h F*O% h %X ."MFBPCR"\AS.seek 0.24ml F*-' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*2 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*;  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *#'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*x @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*` I +86 h I ."MFBPCR"\AS.seek 0ml F*,'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*.  +43 h  #"MFBPCR"\Swish #1 of 2 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *C'\  +1031 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*?'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*J% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*}'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*5% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*" % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *K'V +3472 h 'V ."MFBPCR"\SPE.coast F*4  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0109h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*M% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*g n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *n'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*B  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*m % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*o'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +107 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*h' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F**  +43 h  ."MFBPCR"\ASV.dial :waste1 F*y  +64 h ."MFBPCR"\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*H % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254590 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'z @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'_% h % ."WCR"\CSV.dial between air and CTV C'p ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'R  ."WCR"\CSV.dial CTV C'  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'c'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'E  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'3  +38 h  C'!% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'a @ +75 h @ +50 S)Q% h % ."WCR"\CBV.dial between CSV and waste2b C'8  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'^' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'N  '(  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'f  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'l' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'A' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'F @ +75 h @ +50 S)X% h % ."WCR"\CBV.dial between CSV and waste2b C':  +95 h  C'W% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'k' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\\  '&  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ')'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'6  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'i' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C't  +151 h C'T& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&~% h %\  A(\ % h %X A(s. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (V( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (r& h &  M&=& h &ӷ M&1% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &O( +142 h ( M&j& h & A(2& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &;' +90 h ' A(-& h &  ."WCR"\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' M&S& h & A(#& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &`' +25 h ' ."WCR"\Elbow.hold M&q, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(,- M&$&.,(\ #b h -+ ."WCR"\SE.seek 6.04 Pucks below Top Plate A(.' +27 h ' M&+% h % A(Y& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &<'; +21 h '; M&C& h & A(J& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &?' +22 h ' ."WCR"\SE.seek 7.04 Pucks below Top Plate A(' +36 h ' M&& h & M&5% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +90 h '\  ."WCR"\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M& / +150 h / ."WCR"\CC.seek :open C'M( +416 h ( A(K& h &  A(w& h &  M&g& h &ӷ M&0% h % ."WCR"\Forearm.seek :retract M&G'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&& h & A(L& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b & ' +93 h ' ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&n% h % A(B& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&u'; +21 h '; M&Z& h & A(m& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &b' +22 h ' A(% h % ."downElevator"\SE.seek :down A(D'X +100 (v% h % M&o% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  +2 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 + A(6 + h 1 M&& h & M&{% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.seek :garage M&@'η +91 h 'η .tHand.close M&/ +150 h / M&h& h &- M&>% h %η .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&_1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'R% h % .t\CSV.dial CSR C'  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'E% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C'! +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'a% h %4 .t\CSV.dial :flush C'Q  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  '(  +38 h  +112  .t\CBV.dial :waste1 C'^ @ +76 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'A% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  +38 h  .t\CBV.dial :waste2b C':  +75 h  .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'|% h %4 .t\CSV.dial :flush C'\\  +75 h  .t\CS.seek 5ml C'&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'6% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CBV C't .t\CBV.dial CSV C'T  +38 h +75 .t\CTV.dial :air C'~  +150 h .t\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +507 h '0 .t\CSV.dial :waste C'r +38 h .t\CS.seek 0ml C'='4 +506 h '4 C'1& h &W C'j% h %4 .t\CSV.dial :flush C'2  +38 h  .t\CS.seek 3ml C'-'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'S% h %0 .t\CS.seek 5ml C'#'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +507 h '0 .t\CSV.dial :waste C'+ +75 h .t\CS.seek 0ml C'Y'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'C& h &W C'J% h %4 .t\CSV.dial CSR C'  +150 h  C'5% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C';'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'O' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'`  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'4  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '<'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'.  +113 h  C'?% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'q'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'}  '%  +38 h  +112  .t\CBV.dial :waste1 C'K @ +38 h @ .t\CS.seek 0ml C'w'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'g  +37 h  C'0% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'"  +76 h  .t\CS.seek 0ml C'['4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'n  +38 h  C'B% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +709 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'G  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'v'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'& h &W C'{% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'u'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'*% h %p .t\CS.seek 7ml C'y'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'b +113 h .t\CTV.dial :air .\CS.seek 0ml C'D'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +507 h '4 C'h& h &W C'>% h %4 .t\CSV.dial :flush C'U  +37 h  .t\CS.seek 3ml C'@'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'9% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'H'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'_'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'p& h &W C'% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'z% h %p .t\CS.seek 7ml C'i'i +416 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV C'c  +37 h  +113  .t\CBV.dial :waste1 C'! @ +38 h @ .t\CS.seek 0ml C'3'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'a% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'(  +38 h  .t\CBV.dial :waste2b C'N  +75 h  .t\CS.seek 0ml C'^'4 +766 h '4 #tDisinfecting external Intake with kill C''& h &W C' % h %4 .t\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'f'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A  ' +38 h +75 .t\CTV.dial :intake C'X  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C':' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'F  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'|  +113 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'k' +1372 h ' .t\CSV.dial CBV C'6 +113 h .t\CS.seek 0ml C'&'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&\ ' +90 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&1& h &ӷ M&j% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &=(- +265 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &-'η +91 h 'η .tHand.open M +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C',& h & .tHand.open M&C1 +2 h 1 M&J& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M&/ +150 h / M&`& h &- M&O% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'q(W +416 h (W C'}& h &W .tHand.open M&%1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'0% h %4 .t\CSV.dial CSR C'w  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '"% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'L'[ +463 h '[ .t\CSV.dial :waste C'n +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'B'4 +2010 h '4 #t\Purging external Intake C'[& h &W C'Z% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'm  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'v'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'o& h &W C'% h %4 .t\CSV.dial :flush C'{  +75 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CBV C'u .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'b'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +506 h '0 .t\CSV.dial :waste C'D +38 h .t\CS.seek 0ml C' '4 +507 h '4 C'V& h &W C'h% h %4 .t\CSV.dial :flush C'>  +37 h  .t\CS.seek 3ml C'U'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'9'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'@'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'H +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&M& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &p(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'z( +416 h ( M&& h &ӷ M&E% h % .t\Forearm.seek :retract M&c'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &!(- +265 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &a'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:18 #\Preparing to sleep until Wed Oct 03 06:02:18 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST03-Oct-18 #\Continuing at 06:02CEST03-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:34:48CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST03-Oct-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0109h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*(  +21 h ."MFBPCR"\ATV.dial :air3 F*N % +87 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *''Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +21 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*|% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*)  +31 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*1% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F*#% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*2  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *+'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ARV.dial :carrier F*.  +10 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*K  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +22 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *w'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*"  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*L  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*G  +108 h  ."MFBPCR"delay 7 seconds +700 F*B% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*m n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*v  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0111h25ml.pcr F*b% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *>'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*p % +86 h % F*Q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*a% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*('m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*r  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*1 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*e% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*] n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*t' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*#  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*2'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +108 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*C'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*J n +22 h n ."MFBPCR"delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*`' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*;% h % \\ ."MFBPCR"\AS.seek 0.326ml F*.'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*+ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0113h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*4  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +87 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +21 h % ."MFBPCR"delay 3 seconds +300 F*w% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*[  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *B'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*v % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*u% h % ."MFBPCR"\AS.seek 0.81ml F**'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +21 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*M  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +22 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*c n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*3' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*P' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*N % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*('< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*' n +21 h n ."MFBPCR"delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*X' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0116h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*k  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +87 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *r'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*~% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +21 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*t% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *$'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*.'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*L  +11 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*Z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*v  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*{ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*u% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*x  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.53ml F**' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*y  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +22 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0118h25ml.pcr F*R% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*q % +86 h % F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*Q% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*N  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*8  +64 h  F*P% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*l'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*T'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +10 h  F*$% h %V ."MFBPCR"\AS.seek 0.5ml F*,'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*J n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*; I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*=' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*+ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*?' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +22 h  ."MFBPCR"delay 7 seconds +700 F*K% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*% n +22 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*<'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*w  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*[% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*B % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*n @@T ."MFBPCR"\ASV.dial :SPEdelivery F*Z  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*m  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :ASV F*v n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*o% h % ."MFBPCR"\AS.seek 0.62ml F*{'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*u n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *x'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*b'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *V' +423 h ' ."MFBPCR"\ATV.dial :air3 F**% h % ."MFBPCR"\AS.seek 0.53ml F*D' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +21 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*9' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*U% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*@ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*H' +7161 h ' ."MFBPCR"\ATV.dial :waste F*M n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*E% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*a% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*_' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*I  +22 h  ."MFBPCR"\AS.seek 0ml F*i'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *8'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*P % +108 h % F*3% h %V ."MFBPCR"\AS.seek 0.01ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*(% h % ."MFBPCR"\AS.seek 0.58ml F*'' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*A% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X  +21 h  ."MFBPCR"\AS.seek 0ml F*F'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *|'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\\  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *k' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*6 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *l'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*W n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *T'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*r n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*1  +43 h  F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *)' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*#  +43 h  F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*$  +64 h  F*S% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *,'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*J n +65 h n F*2% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *5' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*C  +43 h  F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *`': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*-  +65 h  F*+% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*= n +65 h n F*4% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *}' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*? I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*K  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *.': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*% n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *g'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*0 n +151 h n ."MFBPCR"\AS.seek 0ml F*w'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*< % +43 h % F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*L'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %0 ."MFBPCR"\AS.seek 0.985ml F*G' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*v I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *u'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*x  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *b' +9988 h ' ."MFBPCR"\ATV.dial AIV F** I +43 h I ."MFBPCR"\AS.seek 0.02ml F*V' +9880 h ' ."MFBPCR"\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*D% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*H'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*E  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*I  +32 h  ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*f  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' @CEST1538431234.71 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *k'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *l'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*T % +108 h % ."MFBPCR"\AS.seek 0ml F*r'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538539339.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )1 h  C' 'e h  P% %~ h  F* *] h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ F*\ 9\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *)  h  .t\SPE.configure SPEconfig F*\ 2 *j% h %V .t\AS.configure ASconfig S)#% F*#,,'T(^20( b h % .t\SS.configure SSconfig C't& S)#.0X 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:35:45CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #11) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&G1 +150 h 1 M&v& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &u'η +90 h 'η .tHand.close M&{/ +150 h / M&x& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +265 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +91 h ' .t\CC.seek :closed C'D(W +416 h (W C'V& h &W .tHand.open M& 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'>% h %4 .t\CSV.dial CSR C'9  +150 h  C'@% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'U'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'M'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +2010 h '4 #t\Purging external Intake C'& h &W C'H% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +76 .t\CTV.dial :intake C'p  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'Q'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'c& h &W C'a% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'I'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'i% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P +113 h .t\CTV.dial :air .\CS.seek 0ml C'3'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'N'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'('4 +507 h '4 C'8& h &W C'^% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C''% h %0 .t\CS.seek 5ml C'A'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +506 h '0 .t\CSV.dial :waste C'X +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'( +416 h ( M&W& h &ӷ M&T% h % .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &/'η +91 h 'η .tHand.open M&d1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:35:50CEST30-Aug-18\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:35:45CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*r% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'n& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&1% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(e% h %X A(~. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (](~ +6 F*)% h %&K ."readyMFB"\ATV.dial :waste2 F*j % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *#' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (t& h &~ M&& h &- M&$% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &S( +166 h ( M&\ & h & A(J& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &2'н +89 h 'н A(,& h &~ .t\SE.seek 12.71 Pucks below Top Plate A(' +137 h ' A(5& h &~ M&Y& h & .t\Elbow.seek 3 M&C( +6 h ( M&;& h & A(+& h &~ .t\Forearm.seek 3 + 300 counts M&O'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &=/ +150 h / ."downElevator"\SE.seek :down M&4& A(-'X h & M&% h %- .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.seek CC M&0(ӷ +46 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +91 h ' .t\CC.seek :closed C'B(W +416 h (W .tHand.open M&Z1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)<+ +6 h + S)w% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +303 h ' .t\Exhaust.select :close S) +7 #t\Sampling 1500.0ml #\Sampling only 25ml to speed simulation .Intake.select :close .\Exhaust.select :close .\SS.reconfigure SSconfig .\SS.hold S)#.0X9 h 9(\\d +100 C'99 h 9@`d +100 C'9 h 9Wdd +100 C'M9 h 9nhd +100 C'9 h 9ld +100 C'H9 h 9pd +100 C'q9 h 9td +100 C'U9 h 9xd +100 C'9 h 9|d +100 C'9 h 9d +100 C' 9 h 9d +100 C'E9 h 9'd +100 C'9 h 9>d +100 C'a9 h 9Td +100 C'p9 h 9kd +100 C' 9 h 9d +100 C'Q9 h 9d +100 C'i9 h 9d +100 C'I9 h 9d +100 C'P9 h 9d +100 C'9 h 9d +100 C'39 h 9 d +100 C'R9 h 9 d +100 C'c9 h 96d +100 C'N9 h 9Ld +100 C'89 h 9bd +100 C'^9 h 9xd +100 C'_9 h 9d +100 C'(9 h 9d +100 C'f9 h 9d +100 C''9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'X9 h 9(d +100 C'F9 h 9>d +100 C'A9 h 9Td +100 C'|9 h 9id +100 C'9 h 9d +100 C'\\9 h 9d +100 C':9 h 9d +100 C'k9 h 9d +100 C'9 h 9d +100 C'D9 h 9d +100 C'T9 h 9  d +100 C'69 h 9 d +100 C'y9 h 9 *d +100 C'!9 h 9 ?d +100 C'79 h 9 Td +100 C'd9 h 9 j d +100 C'W9 h 9 $d +100 C'9 h 9 (d +100 C'l9 h 9 ,d +100 C'r9 h 9 0d +100 C'19 h 9 4d +100 C'e9 h 9 8d +100 C'~9 h 9 % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &9( +143 h ( M&& h & A(M& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(H& h &  .t\SE.seek 6.71 Pucks below Top Plate A(q' +196 h ' M&U& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(E- h -+ .t\SE.seek 7.04 Pucks below Top Plate A(' +27 h ' M&a% h % A(p& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M& '; +21 h '; M&Q& h & A(i& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &I' +22 h ' .t\SE.seek 8.04 Pucks below Top Plate A(P'w +36 h 'w M&& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +90 h '\  .t\Elbow.seek CC M&c(ӷ +142 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'(( +416 h ( A(f& h &  A('& h &  M&& h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &X( +143 h ( M&F& h & A(A& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &|' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\\1 +150 h 1 M&:% h % A(k& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'; +21 h '; M&D& h & A(T& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &6' +22 h ' A(y% h %w ."downElevator"\SE.seek :down A(!'X +100 (7% h % M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +82 h 'X ."downElevator"\SE.coast A(5 +7 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C'l' +7 h ' .t\CSV.dial between air and CTV C'r ` +19 h ` .t\AIV.dial :bypass C'1 { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&~1 +2 h 1 M&)& h & M&j% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.seek :garage M&/'η +91 h 'η .tHand.close M&n/ +150 h / M&\ & h &- M&J% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C'z(W +416 h (W C'5& h &W .tHand.open M&=1 +150 h 1 M&t% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%+ +150 h P%4% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%S' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%2  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P%[ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%}' +2609 h ' ."connect"\PRV.dial CBV P%O  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%K  h .t\PS.seek 0ml P%-' +2610 h ' .t\PSV.dial :air P%Z  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%`  +37 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :mixloop P%v +38 h .t\PS.seek 0ml P%<' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&%& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'?( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&m1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C' & h & M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (@(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &~ M&& h &- M&>% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&M& h & A(H& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &0'н +90 h 'н A(U& h &~ .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +145 h 'Ɛ A(.& h &~ M&E& h & .t\Elbow.seek 3 M&a( +7 h ( M&p& h & A(Q& h &~ .t\Forearm.seek 3 + 300 counts M&i'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(3'X h & M&9% h %- .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +55 h 'X ."downElevator"\SE.coast A(5 +88 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +90 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&I1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'R  +151 h  .t\CSV.dial CTV C'B  +19 h  .t\CS.seek 5ml C'f'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C''' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%^  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%c +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%\\  +150 h  .t\PS.seek :empty P%:' +2610 h ' .t\CBV.dial :waste1 C'k @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'D  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'T @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'y  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'X @ +38 h @ .t\CS.seek 10ml C'7' +2647 h ' .t\CSV.dial CTV C'd  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'r @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'1  +150 h C'e& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&~% h %\  A()% h %X A(j. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (|( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&P& h &ӷ M&6% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&$& h & A(!& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &h' +90 h ' A(V& h &  .t\SE.seek 7.71 Pucks below Top Plate A(' +184 h ' M&n& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &J' +26 h ' .t\Elbow.hold M&W, +6 h , .t\Elbow.reconfigure TightElbow A(/- M&$&.,(\ #b h -+ .t\SE.seek 8.04 Pucks below Top Plate A('w +27 h 'w M&Y% h % A(& h &  .t\Forearm.force -20 .\Forearm.seek 2 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &,'; +20 h '; M&(& h & A(5& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &=' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A(t'f +37 h 'f M&+& h & M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +89 h '\  .t\Elbow.seek CC M&2(ӷ +142 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&O/ +150 h / .t\CC.seek :open C'( +416 h ( A(C& h &  A(K& h &  M&}& h &ӷ M&Z% h % .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &w( +143 h ( M&& h & A(`& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&v, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &]1 +150 h 1 M&%% h % A(o& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'; +21 h '; M&G& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &g' +22 h ' A(z% h %f ."downElevator"\SE.seek :down A('X +100 (% h % M&#% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +72 h 'X ."downElevator"\SE.coast A(5 +18 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +113 h  P%"% h % .t\PS.seek 0.499ml P%m' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%&  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +18 h  P%s% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%> @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %*' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&H1 +2 h 1 M&U& h & M&x% h %\  .t\Elbow.seek FlushPuck::Garage M&.(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.seek :garage M&Q'η +91 h 'η .tHand.close M&{/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'i(W +416 h (W C'& h &W .tHand.open M&a1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  C'?& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%I @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%R  +76 h  .tdelay 2 seconds +200 P%B% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%@' +1094 h ' .t\PSV.dial :air P%l  +38 h  .t\PS.seek 0ml P%f' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%' +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%<% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %A'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%^  +76 h .tdelay 2 seconds +200 P%c% h %r .t\PS.seek 0.475ml P%\\' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%F  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%k'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%D  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%T  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %y' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%X @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%d  +75 h  .t\PS.seek 2.5ml P%7' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%r +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%1' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%e  +75 h  .t\PS.seek 2.5ml P%~' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%) +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%j  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %P' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'6 & +50 h & .t\AWV.select :waste C' +7 P%$% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %!'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'V { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%n  +37 h  .t\PS.seek 2.5ml P%\ ' +2610 h ' .t\PSV.dial :PML P%_ +38 h .t\PS.seek 0ml P%/' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'Y& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& F*( @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*5  .t\AIV.dial :bypass .\PSV.dial PSR P%+ +21 h  ."MFBPCR"delay 20 seconds +92 h P%4% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%h' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *J'6 +66 .t\PSV.dial :waste1 P%S  +38 h  .t\PS.seek 0ml P%[' +1597 h ' .t\PSV.dial PSR P%, +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%= +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%W' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*O  +22 h ' .t\PSV.dial PSR P%; +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*C'ͯ +92 h P%K% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%}' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*Z % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%`  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*2  +21 h  F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *%' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%o +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%G' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*z% h % ."MFBPCR"\AS.seek 0.469ml F*-' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%# +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%t  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*"9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*g n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*|'% +336 h ' .t\PSV.dial PSR P%: +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%& +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%s  +38 h  .t\PS.seek 0ml P%m' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%> +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %U'k +2679 h 'k .t\PSV.dial :waste1 P%x  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %E' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*p I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *v'V +850 h ' .t\PSV.dial :air P%{  +112 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %b' +5106 h ' .t\PSV.dial :air P%Q  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %0' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*N  +43 h  F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *9'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*a' +171 h ' .t\PSV.dial :mixloop P%3 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %?' +619 h ' ."MFBPCR"\ATV.dial :waste F*I n +129 h n ."MFBPCR"\AS.seek :empty F*8'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *B' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F**  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *l'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'@  +38 h  C''% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'<' +1625 h ' ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV C'^  +38 h  +113  .t\CBV.dial :waste1 C'c @ +38 h @ .t\CS.seek 0ml C'A'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*F  +22 h ."MFBPCR"delay 3 seconds +300 F*\\% h %_ ."MFBPCR"\AS.seek 0.434ml F*D'ݏ +110 h '4 .t\CSV.dial CSR C'T  +38 h  C'k% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*X  +22 h F*y% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*d'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*r% h %& ."MFBPCR"\AS.seek 0.092ml F*7'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'e' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'1  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C')'4 +2647 h '4 C'j& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +7 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.seek CC M&P' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'!( +416 h ( M&n& h &ӷ M&% h % .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &\ (- +264 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&+1 +150 h 1 M&4% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'& h & M&% h %\  A(S% h %X A(u. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (h(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (,& h &~ M&[& h &- M&=% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&O& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &;'н +90 h 'н A(K& h &~ ."WCR"\SE.seek 10.71 Pucks below Top Plate A(J' +155 h ' A(W& h &~ M&Z& h & ."WCR"\Elbow.seek 3 M&C( +6 h ( M&`& h & A(2& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&]'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &}/ +100 h '&0 ."MFBPCR"delay 3 seconds +50 h / ."downElevator"\SE.seek :down M&& h & A(o'X M&% h %- ."WCR"\Forearm.seek :retract M&z'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&%& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &G' +16 ."MFBPCR"\ATV.dial :waste2 F*# % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +32 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&t1 +111 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *"' +17 h 1 M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)-+ +6 h + S):% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'&  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +216 h ' ."WCR"\SSV.dial CBV S)m  +150 h  ."WCR"\CTV.dial :intake C'|  +76 h  #"WCR"Waiting for external sampler to finish priming... .delayUntil "08:37:18.74" +18543 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*H n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*' +9396 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'x% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *p'\  +1032 h '\  F*M% h %\  ."MFBPCR"\AS.seek 0.5ml F*{'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*E% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*U% h %\  ."MFBPCR"\AS.seek 0.5ml F*b'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*N% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *.' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*v % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*Q  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*3  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*0'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 1ml F*I' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*a n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F**  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *9'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*B  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*^'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\\  +107 h  ."MFBPCR"delay 7 seconds +700 F*T% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*y  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 F*d  +65 h ."MFBPCR"\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*1% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254620 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'6  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'j @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)$% h % ."WCR"\CBV.dial between CSV and waste2b C'i  +94 h  C'n% h % ."WCR"\CSV.dial between air and CTV C'q ` +19 h ` ."WCR"\CS.seek 7.288ml C'P' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Y  '  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'+  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'_  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'4  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'u' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)S% h % ."WCR"\CBV.dial between CSV and waste2b C'=  +95 h  C'5% h % ."WCR"\CSV.dial between air and CTV C'[ ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'K  'O  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'W'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'Z  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '\ ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'2  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'/  +37 h  C'`% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'}' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S);% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'# ` +56 h ` ."WCR"\CS.seek 7.288ml C'%' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C']  ."WCR"\CSV.dial CTV C'C  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'o'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'J  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '7' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'!  +151 h C'z& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&t% h %\  A(g% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (&& h &  M&:& h &ӷ M&G% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &h( +143 h ( M&& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(-& h &  ."WCR"\SE.seek 8.71 Pucks below Top Plate A(s' +175 h ' M& & h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &|' +26 h ' ."WCR"\Elbow.hold M&m, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(H- M&$&.,(\ #b h -+ ."WCR"\SE.seek 9.04 Pucks below Top Plate A(x'f +27 h 'f M&>% h % A("& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&M'; +20 h '; M&p& h & A(E& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &{' +22 h ' ."WCR"\SE.seek 10.04 Pucks below Top Plate A(U'U +37 h 'U M&& h & M&N% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +89 h '\  ."WCR"\Elbow.seek CC M&v(ӷ +143 h (ӷ M&.& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&3/ +150 h / ."WCR"\CC.seek :open C'( +415 h ( A(& h &  A(0& h &  M&a& h &ӷ M&I% h % ."WCR"\Forearm.seek :retract M& '\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&*& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &R' +92 h ' ."WCR"\Elbow.hold M&B, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &'1 +150 h 1 M&9% h % A( & h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&f'; +20 h '; M&@& h & A(c& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &<' +22 h ' A(% h %U ."downElevator"\SE.seek :down A(\\'X +100 (T% h % M&^% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +62 h 'X ."downElevator"\SE.coast A(5 +28 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&k1 +2 h 1 M&y& h & M&X% h %\  .t\Elbow.seek FlushPuck::Garage M&l(- +166 h (- M&D& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&1/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +264 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'$(W +416 h (W C'i& h &W .tHand.open M&n1 +150 h 1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'% h % .t\CSV.dial CSR C'P  +37 h  C'+% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'~'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '_% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '4'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'u& h &W C'% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '='p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C'[% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CTV C'O  .t\CTV.dial CSV C'K  +38 h  +112  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'Z'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'W& h &W C'2% h %4 .t\CSV.dial :flush C'/  +75 h  .t\CS.seek 5ml C'`'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'}% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C';  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'#& h &W C'% h %4 .t\CSV.dial :flush C'C  +75 h  .t\CS.seek 5ml C']'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +38 h @ C'J% h %p .t\CS.seek 7ml C'o'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! 'z  +38 h +75 .t\CTV.dial :air C't  +151 h .t\CS.seek 0ml C'g'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'& +38 h .t\CS.seek 0ml C':'4 +506 h '4 C'G& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'-'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C' % h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'H'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'> +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'p& h &W C'E% h %4 .t\CSV.dial CSR C'  +150 h  C'N% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C' 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'h' +416 h ' ."connect"\CSV.dial CBV C's .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'|  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'.' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Q  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'x  +113 h  C'{% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'm'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CTV C'b  .t\CTV.dial CSV C'  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'0'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'a  +37 h  C'I% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig C'#P d@b .t\CS.seek 4ml C'*'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'v  +76 h  .t\CS.seek 0ml C'''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'9  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '@'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'T'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +506 h '0 .t\CSV.dial :waste C'A +38 h .t\CS.seek 0ml C'k'4 +506 h '4 #t\Flushing upper manifold C'y& h &W C'X% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'D% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'< +113 h .t\CTV.dial :air .\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'B +38 h .t\CS.seek 0ml C'1'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'F  +37 h  .t\CS.seek 3ml C'r'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +37 h @ C')% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'i +76 h .t\CS.seek 0ml C'n'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'q& h &W C'Y% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'P'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +38 h @ C'% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  '~  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'4'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'u& h &W C'% h %4 .t\CSV.dial :flush C'S  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C'[% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'O  +38 h  .t\CBV.dial :waste2b C'K  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C',& h &W C'W% h %4 .t\CSV.dial CSR C'2  +38 h  C'/% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'Z'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'\ ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} '`  +38 h +75 .t\CTV.dial :intake C';  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'%' +1372 h ' .t\CSV.dial CBV C'J +113 h .t\CS.seek 0ml C']'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&g' +90 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'( +416 h ( M&G& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &-'η +91 h 'η .tHand.open M&1 +150 h 1 M&H% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&p1 +2 h 1 M&E& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&N& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&s/ +150 h / M&|& h &- M&h% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +264 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C'b& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'a& h &W C'I% h %4 .t\CSV.dial CSR C'0  +113 h  C'*% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'9 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Purging external Intake C''& h &W C'@% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'c  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'T'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'A  +76 h #t\Flushing upper manifold C'^& h &W C'y% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'U% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f +113 h .t\CTV.dial :air .\CS.seek 0ml C'<'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'd'0 +506 h '0 .t\CSV.dial :waste C'\\ +38 h .t\CS.seek 0ml C'B'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'F'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'1 @ +37 h @ C'j% h %0 .t\CS.seek 5ml C')'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'r'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'i'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +7 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&+/ +150 h / .t\CC.seek :open C'( +416 h ( M&P& h &ӷ M&_% h % .t\Forearm.seek :retract M&~'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&7& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&S1 +150 h 1 M&4% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:34 #\Preparing to sleep until Fri Oct 05 06:02:34 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST05-Oct-18 #\Continuing at 06:02CEST05-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:37:17CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST05-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0309h25ml.pcr F*[% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*O  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *,'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*/'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*`'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *C'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*x  +11 h  F*{% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*a  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *0'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F**  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*v  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*9' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*c n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*A'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*^  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*X' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*T  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*D % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0311h25ml.pcr F*<% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*d  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +21 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*l% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*+% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *.'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*u  +31 h  ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *4' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +21 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*C  +10 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +22 h ."MFBPCR"delay 2 seconds +200 F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*G I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*!' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*"'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*|' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*h  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*:  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*x'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*> % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0313h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F**'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*0% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*'  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*9  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +32 h  ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +21 h F*f% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\\  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*.% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +21 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *7'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*~ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*4' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*[ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.53ml F*(' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*K % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*W  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*, n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*Z'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*`  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0316h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*%  +22 h ."MFBPCR"\ATV.dial :air3 F*J % +86 h % F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *&'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*{'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*x'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*@'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +21 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*X n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*U' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.53ml F*D' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*d  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\\  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*1 n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*F'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*)  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*+'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0318h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*P  +22 h ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*S% h %Z ."MFBPCR"\AS.seek 0.81ml F*n'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*7'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +21 h F*e% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*=  +65 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*}  +31 h  ."MFBPCR"\ATV.dial :air3 F*Z % +87 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*t' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*!  +42 h  ."MFBPCR"\ARV.dial :carrier F*g  +11 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*|% h %Fj ."MFBPCR"\AS.seek 0.524ml F*:' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*> % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*x'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*'% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*9 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*@  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*c  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *A' +424 h ' ."MFBPCR"\ATV.dial :ASV F*T n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*k  +43 h  F*^% h % ."MFBPCR"\AS.seek 0.62ml F*X'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*f n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *y'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*<'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*U % +43 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.71ml F*1' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h % H ."MFBPCR"\AS.seek 0.735ml F*)' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*F% h % Ű ."MFBPCR"\AS.seek 0.72ml F*l' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*r n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *P' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +21 h F*_% h % ."MFBPCR"\AS.seek 0.579ml F*3' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %  ."MFBPCR"\AS.seek 0.604ml F*u'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*n' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*[ n +85 h n ."MFBPCR"\ASV.dial :wasteR F*e  +22 h  ."MFBPCR"\AS.seek 0ml F*7'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *='V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*( % +107 h % F*4% h %V ."MFBPCR"\AS.seek 0.01ml F*W' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*O% h % ."MFBPCR"\AS.seek 0.58ml F*,' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h % q ."MFBPCR"\AS.seek 0.605ml F*}'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*`% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*/' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*;  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*C  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *%' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *\ '^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +202 h 'R ."MFBPCR"\SPE.coast F*6  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*& n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*G  +43 h  F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *o' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *g': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*H  +65 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*E n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *N' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*p  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *|': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*-  +65 h  F*>% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*: n +64 h n F*Q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *b' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*{ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *x': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *a'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*I n +151 h n ."MFBPCR"\AS.seek 0ml F*0'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F**  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*M % +43 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F* '0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %0 ."MFBPCR"\AS.seek 0.985ml F* ' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*T I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *^'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *f'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *y'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*U% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *<' +9988 h ' ."MFBPCR"\ATV.dial AIV F*D I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*R'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*\\% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*B  +22 h ."MFBPCR"\ATV.dial :air3 F*1 % +43 h % F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*m  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*_  +65 h  F*P% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *q'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +31 h  ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*7% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *K' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*4% h % ."MFBPCR"\AS.seek 0.81ml F*='> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*2% h %m ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' @CEST1538604049.83 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *\ '(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*z % +107 h % ."MFBPCR"\AS.seek 0ml F*&'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538712154.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )G h  C' 'V h  P% %t h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *o  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'!& S)#.0X  .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &|'\ h ."rdySampler"\CSV.dial :waste1 C': h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)Q h  S)  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %{' +20 h '\  ."closeHand"Hand.close M&b/ .t\Elbow.jog 1500 M&* +18 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:38:14CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #12) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +150 h 1 M&T& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &k(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &f'η +91 h 'η .tHand.close M&X/ +150 h / M&& h &- M&U% h %η .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'& h &W .tHand.open M&R1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'j& h &W C'% h %4 .t\CSV.dial CSR C')  +151 h  C'r% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'F'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'l% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'Y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'+& h &W C'% h %4 .t\CSV.dial :flush C'm  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '_' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P '.  h +75 .t\CTV.dial :intake C'q  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'S'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'3  +75 h #t\Flushing upper manifold C'~& h &W C'% h %4 .t\CSV.dial :flush C'u  +75 h  .t\CS.seek 5ml C'e'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +37 h @ C'7% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CBV C'( .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'4'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'O'4 +507 h '4 C'=& h &W C'% h %4 .t\CSV.dial :flush C'2  +38 h  .t\CS.seek 3ml C'W'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +38 h @ C',% h %0 .t\CS.seek 5ml C'`'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'}'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'; +75 h .t\CS.seek 0ml C'/'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +6 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&J' +91 h ' .tHand.close M&%/ +150 h / .t\CC.seek :open C'6( +416 h ( M&w& h &ӷ M&z% h % .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &?'η +91 h 'η .tHand.open M&81 +150 h 1 M&i% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:38:18CEST30-Aug-18\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:38:14CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*&% h %&K #tLoading sh1 puck from tube 3 into Collection Clamp #"readyMFB"Readying the MFB C'9& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&G% h %\  A(V% h %X A(t. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +7 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +193 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &~ M&$& h &- M&H% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +166 h ( M&g& h & A(E& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A(& h &~ .t\SE.seek 9.71 Pucks below Top Plate A(s' +166 h ' A(N& h &~ M&p& h & .t\Elbow.seek 3 M&"( +6 h ( M& & h & A(h& h &~ .t\Forearm.seek 3 + 300 counts M&>'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &:/ +150 h / ."downElevator"\SE.seek :down A(Q'X M&-& h & M&% h %- .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.seek CC M&I(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&@1 +150 h 1 M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)M+ +6 h + S)0% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +150 h  .t\CSV.dial CSR C's  +76 h  C'"% h %4 #t\Pulling 1ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1ml C'#P d@b 'Q' +350 h ' .tdelay 5 seconds +500 .\CSR.select :air C' +7 'b% h % .t\CS.seek 6ml C'6' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'@ +150 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b '|' +710 h ' .t\CSV.dial CSR C'0  +151 h  C'% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'v' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %  .t\CS.seek 4.5ml C''` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'M  +37 h  .t\CS.seek 1.275ml C'' +919 h ' #tHeating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'T   '{9 h 9d +100 C'^9 h 9d +100 C'A9 h 91d +100 C'9 h 9J d +100 C' 9 h 9bd +100 C'9 h 9zd +100 C'U9 h 9d +100 C'9 h 9d +100 C']9 h 9 d +100 C'D9 h 9$d +100 C'<9 h 9(d +100 C'k9 h 9 ,d +100 C'X9 h 9$0d +100 C' 9 h 9<4d +100 C'9 h 9S8d +100 C'R9 h 9kd +100 C'9 h 9Td +100 C'q9 h 9kd +100 C'u9 h 9d +100 C'S9 h 9d +100 C'79 h 9d +100 C'e9 h 9d +100 C'(9 h 9d +100 C'[9 h 9d +100 C'49 h 9 d +100 C'9 h 9 d +100 C'~9 h 96d +100 C'K9 h 9Ld +100 C'=9 h 9bd +100 C'9 h 9xd +100 C'n9 h 9d +100 C'O9 h 9d +100 C'Z9 h 9d +100 C',9 h 9d +100 C'29 h 9d +100 C'W9 h 9d +100 C'}9 h 9d +100 C';9 h 9(d +100 C'9 h 9>d +100 C'`9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'C9 h 9d +100 C'9 h 9d +100 C'%9 h 9d +100 C'19 h 9d +100 C'\\9 h 9d +100 C'z9 h 9  d +100 C'J9 h 9 d +100 C'd9 h 9 *d +100 C'9 h 9 ?d +100 C'L9 h 9 Td +100 C'89 h 9 j d +100 C'w9 h 9 $d +100 C'#9 h 9 (d +100 C'\ 9 h 9 ,d +100 C'&9 h 9 0d +100 C'G9 h 9 4d +100 C'V9 h 9 8d +100 C't9 h 9 9 h 9 d +100 C's9 h 9 1d +100 C'"9 h 9 Dd +100 C'b9 h 9 Xd +100 C'Q9 h 9 kd +100 C'@9 h 9 ~d +100 C'69 h 9 d +100 C'09 h 9 d +100 C'9 h 9 d .t\CH.hold 5:00,85C C'|   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'v'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'M @ +113 h @ .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 5ml C'{'p +1372 h 'p .t\CSV.dial CTV C'^  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'A @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'U +113 h .t\CBV.dial CSV C'  +19 h C']% h %4 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'<  +38 h C'k% h %p .t\CS.seek 7ml C'X'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +38 h @ .t\CS.seek 10ml C'' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'R ` +132 h ` #tLysing Complete C'B& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&j% h %\  A(y% h %X A(r. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&l& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &)( +142 h ( M&Y& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(& h &  .t\SE.seek 9.71 Pucks below Top Plate A(m' +166 h ' M&F& h & A(.& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &P' +26 h ' .t\Elbow.hold M&3, +6 h , .t\Elbow.reconfigure TightElbow A(_- M&$&.,(\ #b h -+ .t\SE.seek 10.04 Pucks below Top Plate A(/'U +27 h 'U M&% h % A(q& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&u'; +20 h '; M&S& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &e' +22 h ' .t\SE.seek 11.04 Pucks below Top Plate A(('D +37 h 'D M&[& h & M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&~(ӷ +143 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'O( +415 h ( A(Z& h &  A(,& h &  M&2& h &ӷ M&W% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +143 h ( M&& h & A(`& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&% h % A(%& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &1'; +20 h '; M&\\& h & A(z& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +22 h ' A(d% h %D ."downElevator"\SE.seek :down A('X +100 (L% h % M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +52 h 'X ."downElevator"\SE.coast A(5 +38 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'# @ +19 h @ .t\CS.seek 10ml C'\ ' +6 h ' .t\CSV.dial between air and CTV C'& ` +19 h ` .t\AIV.dial :bypass C'G { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'V& h & .tHand.open M&t1 +2 h 1 M&o& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&i(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.seek :garage M&?'η +91 h 'η .tHand.close M&9/ +150 h / M&g& h &- M&E% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +264 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'N& h &W .tHand.open M&:1 +150 h 1 M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%h +151 h P%-% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%' +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C's  P%>  h +75 .t\PSV.dial :mixloop P%" +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%b  h .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%@  +37 h  .t\PS.seek 2.5ml P%6' +2610 h ' .t\PSV.dial :mixloop P%0 +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%|  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%M' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +6 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'{( +416 h ( M&U& h &ӷ M&% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &D'η +91 h 'η .tHand.open M& 1 +150 h 1 M&T% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'R& h & M&B% h %\  A(j% h %X A(y. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (r(~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&l& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Y( +166 h ( M&& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &I'н +89 h 'н A(F& h &~ .t\SE.seek 8.71 Pucks below Top Plate A(.' +175 h ' A(_& h &~ M&x& h & .t\Elbow.seek 3 M&( +7 h ( M&q& h & A(S& h &~ .t\Forearm.seek 3 + 300 counts M&7'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &[/ +150 h / ."downElevator"\SE.seek :down A(4'X M&+& h & M&)% h %- .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.seek CC M&P(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +59 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +91 h ' .t\CC.seek :closed C'/(W +415 h (W .tHand.open M&e1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'Z'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C',' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'2'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%` +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%~ +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%C  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'% @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'\\  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'z @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'd  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'} ` +18 h ` .t\CSV.dial :air C'; @ +38 h @ .t\CS.seek 10ml C'L' +2647 h ' .t\CSV.dial CTV C'8  +38 h  .t\CS.seek 0ml C'#'4 +2647 h '4 .t\CBV.dial :waste1 C'& @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'V& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&t% h %\  A(o% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (1& h &  M&(& h &ӷ M&J% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &$( +142 h ( M&H& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &  .t\SE.seek 10.71 Pucks below Top Plate A(' +155 h ' M&9& h & A(g& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &E' +25 h ' .t\Elbow.hold M&w, +7 h , .t\Elbow.reconfigure TightElbow A(?- M&$&.,(\ #b h -+ .t\SE.seek 11.04 Pucks below Top Plate A(5'D +26 h 'D M&p% h % A(i& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'; +21 h '; M&O& h & A(N& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &:' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(!'3 +36 h '3 M&h& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C's( +416 h ( A("& h &  A(b& h &  M&& h &ӷ M&@% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &0( +142 h ( M&6& h & A(|& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(^& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &A'; +20 h '; M& & h & A(*& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &a' +23 h ' A(% h %3 ."downElevator"\SE.seek :down A(U'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%k  +113 h  P%v% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%T +37 h .t\PRV.dial :air P%]  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%R  +38 h .t\CBV.dial AIV C'B  +37 h .t\PRV.dial between CBV and air P%j  +19 h  .t\PRV.dial :air P%y  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %l' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %D' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&F& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&_(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&S'η +91 h 'η .tHand.close M&X/ +150 h / M&[& h &- M&+% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +264 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +91 h ' .t\CC.seek :closed C'7(W +416 h (W C'.& h &W .tHand.open M&1 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  C'{& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%e @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%3  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P% % h % .t\PS.seek 1.499ml P%u' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%r' +1094 h ' .t\PSV.dial :air P%\  +38 h  .t\PS.seek 0ml P%Z' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%, +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%W  +38 h  .tdelay 2 seconds +200 P%M% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %`'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%~% h %r .t\PS.seek 0.475ml P%C' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%\\  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%z  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %d' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%} +76 h .t\PTV.dial :PRV,avoiding: :puck P%; @ +75 h @ .t\PS.seek 0ml P%2' +2610 h ' .t\PSV.dial :air P%8  +75 h  .t\PS.seek 2.5ml P%L' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%& +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%V  +75 h  .t\PS.seek 2.5ml P%t' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%o +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%1% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %(' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'J & +50 h & .t\AWV.select :waste C' +6 P%H% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %P' +1313 h ' .t\PSV.dial :air P%9  +38 h  .t\PS.seek 2.5ml P%g' +2609 h ' .t\PSV.dial :PML P%? +38 h .t\PS.seek 0ml P%n' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'p& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'i& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*N  P%h +22 h  ."MFBPCR"delay 20 seconds +91 h P%-% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*$ I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *E'6 +65 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%'' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%5' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%w' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*>  +22 h ' .t\PSV.dial PSR P% +21 h  F*/% h %6 ."MFBPCR"\AS.seek 0.329ml F*"'ͯ +92 h P%b% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*@ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%|  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +217 ."MFBPCR"\ATV.dial :AIV F** I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*Q' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%k' +740 h ' ."MFBPCR"delay 5 seconds +500 F*v9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*a n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%T' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%] +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%R' +2610 h ' .t\PSV.dial PSR P%B +113 h P%j% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%l' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %F'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %x' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*q I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%X  +113 h  .t\PS.seek 2.5ml P%[' +2610 h ' .t\PSV.dial :mixloop P%+ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %<' +5107 h ' .t\PSV.dial :air P%S  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %I' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*K  +43 h  F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *)'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*s I +43 h I F*.% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%4 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %{' +618 h ' ."MFBPCR"\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.seek :empty F*='R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*3  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*D  +43 h F*u% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *\ '_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'r  +38 h  C',% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'M' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Z  '  +38 h  +112  .t\CBV.dial :waste1 C'~ @ +38 h @ .t\CS.seek 0ml C'`'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*C% h %_ ."MFBPCR"\AS.seek 0.434ml F*\\'ݏ +110 h '4 .t\CSV.dial CSR C'z  +38 h  C'%% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*;  +22 h F*d% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*8'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*2 n +108 h n F*&% h %& ."MFBPCR"\AS.seek 0.092ml F*L'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'V' +1625 h ' ."connect"\CSV.dial CTV C'G  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'o'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&1& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +6 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.seek CC M&(' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&9& h &ӷ M&P% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +264 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &N'η +91 h 'η .tHand.open M&h1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'$& h & M&% h %\  A( % h %X A(f. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&'& h &- M&:% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &5( +166 h ( M&>& h & A(/& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & 'н +89 h 'н A(b& h &~ ."WCR"\SE.seek 7.71 Pucks below Top Plate A(E' +185 h ' A(w& h &~ M&@& h & ."WCR"\Elbow.seek 3 M&"( +6 h ( M&|& h & A(& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +70 h '&0 ."MFBPCR"delay 3 seconds +80 h / ."downElevator"\SE.seek :down M&^& A(6'X h & M&*% h %- ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +35 ."MFBPCR"\ATV.dial :waste2 F* % +14 h (ӷ M&0& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +29 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +62 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&!1 +82 h 'R ."MFBPCR"\ATV.dial :air2 F*A  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *v' +47 h 1 M&a% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)Q+ +6 h + S)% h % C'k& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C']  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %  ."MFBPCR"\AS.seek 0.604ml F*g'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*@% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*|' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*n  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b **'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*5 % +107 h % F* % h %V ."MFBPCR"\AS.seek 0.59ml F*0' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*E' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*" I +86 h I F*6% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*L % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +21 h F*a% h % ."MFBPCR"\AS.seek 0.23ml F*' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*Q' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*j n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*B  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*T @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*v'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *q'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*X'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*x% h %(A ."MFBPCR"\AS.seek 0.6ml F*+'\  +1031 h '\  F*F% h %\  ."MFBPCR"\AS.seek 0.5ml F*<'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*K% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *_' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +3472 h 'V ."MFBPCR"\SPE.coast F*S  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0509h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*.  +64 h  ."MFBPCR"\ARV.dial :carrier F*4  +11 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F*I'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +22 h ."MFBPCR"delay 2 seconds +200 F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*#' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*~ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +107 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.65ml F*2' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 F*8  +64 h ."MFBPCR"\ARV.dial :air1 F*&  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254649 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'J  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'Y @ +75 h @ +50 S)H% h % ."WCR"\CBV.dial between CSV and waste2b C'7  +94 h  C'9% h % ."WCR"\CSV.dial between air and CTV C'm ` +19 h ` ."WCR"\CS.seek 7.288ml C'(' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'p  'P  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 't'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'h  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'i' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'?  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'-  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'f' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'$ @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C':  +94 h  C'N% h % ."WCR"\CSV.dial between air and CTV C'' ` +56 h ` ."WCR"\CS.seek 7.288ml C'/' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'b  '>  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'w'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'@  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'g' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'n  +37 h  C'|% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'*  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'0' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '"  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '6'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'E  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&!% h %\  A(a% h %X A(A. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (k( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (]& h &  M&& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&& h & A(j& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &B' +89 h ' A(Q& h &  ."WCR"\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ M&R& h & A(y& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M& , +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+Ɛ ."WCR"\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M&% h % A(v& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'; +21 h '; M&q& h & A(x& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +22 h ' ."WCR"\SE.seek 13.04 Pucks below Top Plate A(F'" +36 h '" M&+& h & M&K% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&_& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ ."WCR"\Forearm.seek CC M&.' +91 h ' ."WCR"Hand.close M&4/ +150 h / ."WCR"\CC.seek :open C'l( +416 h ( A([& h &  A(I& h &  M&& h &ӷ M&e% h % ."WCR"\Forearm.seek :retract M&3'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &s( +142 h ( M&D& h & A(u& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &,1 +150 h 1 M&)% h % A(W& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&Z'; +21 h '; M&r& h & A(~& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &M' +22 h ' A(#% h %" ."downElevator"\SE.seek :down A(C'X +100 (z% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +33 h 'X ."downElevator"\SE.coast A(5 +56 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&%1 +2 h 1 M&d& h & M&;% h %\  .t\Elbow.seek FlushPuck::Garage M&\ (- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.seek :garage M&&'η +91 h 'η .tHand.close M&G/ +150 h / M&2& h &- M&}% h %η .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'H(W +416 h (W C'7& h &W .tHand.open M&91 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'P& h &W C'p% h % .t\CSV.dial CSR C'(  +38 h  C'h% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C't'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '?% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '-'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'f& h &W C'$% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ':'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +37 h @ C''% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'>  .t\CTV.dial CSV C'b  +38 h  +113  .t\CBV.dial :waste1 C'/ @ +75 h @ .t\CS.seek 0ml C'@'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'w& h &W C'% h %4 .t\CSV.dial :flush C'n  +75 h  .t\CS.seek 5ml C'|'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'g @ +37 h @ C'% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'*  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing upper manifold C'& h &W C'^% h %4 .t\CSV.dial :flush C'"  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'E% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'!  +151 h .t\CS.seek 0ml C'a'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C'] +38 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'j  +37 h  .t\CS.seek 3ml C'Q'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'R% h %0 .t\CS.seek 5ml C'y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'v +76 h .t\CS.seek 0ml C'U'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'q& h &W C'x% h %4 .t\CSV.dial CSR C'+  +151 h  C'K% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'B'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'_' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'S  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'X% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '4' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'<  '  +38 h  +113  .t\CBV.dial :waste1 C'[ @ +38 h @ .t\CS.seek 0ml C'I'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'e% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'D'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +709 h ' ."connect"\CSV.dial CTV C'.  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C','4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C')  +37 h  C'W% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'r'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3  '# +37 h +76 .t\CTV.dial :air C's  +150 h .t\CS.seek 0ml C'z'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'%'4 +507 h '4 #t\Flushing upper manifold C'd& h &W C';% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'Z'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +37 h @ C'\\% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'k'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C'2& h &W C'}% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'&'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'o% h %0 .t\CS.seek 5ml C'\ 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +507 h '0 .t\CSV.dial :waste C'7 +75 h .t\CS.seek 0ml C'9'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'm& h &W C'P% h %4 .t\CSV.dial :flush C'p  +37 h  .t\CS.seek 5ml C'('p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'Y% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV C'?  .t\CTV.dial CSV C't  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'-'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'f& h &W C'$% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +37 h @ C''% h %p .t\CS.seek 7ml C':'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'>  +38 h  .t\CBV.dial :waste2b C'b  +75 h  .t\CS.seek 0ml C'/'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'w% h %4 .t\CSV.dial CSR C'  +38 h  C'n% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'@'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'g' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '|  +37 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'*' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'5  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 .t\CSV.dial CSR C'^  +113 h  C'"% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'0' +1372 h ' .t\CSV.dial CBV C'E +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&a' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'A( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Q'η +91 h 'η .tHand.open M&y1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&q1 +2 h 1 M&x& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &+(- +7 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&S'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'<& h &W .tHand.open M&1 +150 h 1 M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'e% h %4 .t\CSV.dial CSR C'I  +113 h  C'D% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'.'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'u'[ +463 h '[ .t\CSV.dial :waste C') +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +2010 h '4 #t\Purging external Intake C',& h &W C'r% h %4 .t\CSV.dial :flush C'3  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '#' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C's +75 h .t\CTV.dial :intake C'~  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'z'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'`  +75 h #t\Flushing upper manifold C'& h &W C'd% h %4 .t\CSV.dial :flush C';  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +37 h @ C'F% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'M'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +507 h '0 .t\CSV.dial :waste C'C +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'k& h &W C'2% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'% h %0 .t\CS.seek 5ml C'o'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'&'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C'1 +75 h .t\CS.seek 0ml C'7'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&l& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&9' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'Y( +416 h ( M&(& h &ӷ M&?% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow M&$&.,(\ #b .t\Elbow.seek :garage M&(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &$'η +91 h 'η .tHand.open M& 1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:49 #\Preparing to sleep until Sun Oct 07 06:02:49 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST07-Oct-18 #\Continuing at 06:02CEST07-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:39:44CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST07-Oct-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0509h25ml.pcr F*'% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*>  +21 h ."MFBPCR"\ATV.dial :air3 F*b % +87 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*n'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*|'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F**  +65 h  F*^% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *"'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +31 h  ."MFBPCR"\ATV.dial :air3 F*] % +87 h % F*J% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0511h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*+'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*D  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*)' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*u  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*r'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +108 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +21 h n ."MFBPCR"delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.6ml F*`'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*%  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0511h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*8  +22 h ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *}'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*\ % h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*p  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*f  +32 h  ."MFBPCR"\ATV.dial :air3 F*m % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *-' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0513h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*"  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*]  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*J  +21 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *H'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*a' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*y  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*j'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*q'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*x n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*B% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*v % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0513h25ml.pcr F*+% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*D'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +31 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +21 h F*k% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0516h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ARV.dial :carrier F*l  +10 h  F*_% h %V ."MFBPCR"\AS.seek 0.5ml F*4'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*t n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*-' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*' I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*b % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*>'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*w  +107 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*n  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*5  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*g'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0516h25ml.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*0  +22 h ."MFBPCR"\ATV.dial :air3 F*E % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*H'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*U % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*X'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0518h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*u  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*# n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *s'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*; n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*Z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*T  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*8  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +22 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*G n +21 h n ."MFBPCR"delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*1' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*&  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*P% h % \\ ."MFBPCR"\AS.seek 0.326ml F*h'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0518h25ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*(  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*V% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*b  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *5' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*g'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*E% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0520h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*a  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*x n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Q'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*B I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*v % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*X' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +22 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0520h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*) @@T ."MFBPCR"\ASV.dial :SPEdelivery F*r  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*~  +151 h F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *`' +423 h ' ."MFBPCR"\ATV.dial :ASV F*z n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*%  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*;'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*Z n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *T'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*M'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*F % +43 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +423 h ' ."MFBPCR"\ATV.dial :air3 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*C' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*G' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*o' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*\ ' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*& n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*P% h % zG ."MFBPCR"\AS.seek 0.365ml F*1' +7161 h ' ."MFBPCR"\ATV.dial :waste F*l n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*p % +43 h % F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*?% h % ."MFBPCR"\AS.seek 0.579ml F*4' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*f'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*$% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *:'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*-% h %V ."MFBPCR"\AS.seek 0.01ml F*w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*>% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +21 h F*|% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*n' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F** n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*5'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *^'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*"  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *0' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*E n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *g'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*J  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*!'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*] n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *6' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*y  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *a': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*R% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*x n +65 h n F*j% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *K' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*q  +43 h  F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*Q  +65 h  F*v% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *+'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*S% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*X I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*[  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*e n +151 h n ."MFBPCR"\AS.seek 0ml F*I'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*D  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*.% h % ."MFBPCR"\AS.seek 0.96ml F*u'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %0 ."MFBPCR"\AS.seek 0.985ml F*3' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*s% h % ."MFBPCR"\AS.seek 0.97ml F*#'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*z I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Z'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*T  +43 h  F*F% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *M' +9988 h ' ."MFBPCR"\ATV.dial AIV F*\\ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*k' +9880 h ' ."MFBPCR"\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*C% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*&% h %Z ."MFBPCR"\AS.seek 0.81ml F*P'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*h% h %n ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +64 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F*9 % +86 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*-% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*@  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V @CEST1538776802.51 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*J n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *g'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*]'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + &6 P%5 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538884969.89 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' ' h  P% %! h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *6  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)y% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:40:42CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #13) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C's& h & .tHand.open M&31 +150 h 1 M&z& h &_ M&#% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &%(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &Z'η +91 h 'η .tHand.close M&;/ +150 h / M&T& h &- M&F% h %η .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +265 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'C(W +415 h (W C'k& h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'}% h %4 .t\CSV.dial CSR C'o  +151 h  C'&% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '\ % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'l'[ +463 h '[ .t\CSV.dial :waste C'P +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Purging external Intake C'h& h &W C'1% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( '_  h +76 .t\CTV.dial :intake C'm  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C' '4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'4  +76 h #t\Flushing upper manifold C't& h &W C'$% h %4 .t\CSV.dial :flush C'f  +75 h  .t\CS.seek 5ml C'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'L% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +113 h .t\CTV.dial :air .\CS.seek 0ml C'-'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +506 h '0 .t\CSV.dial :waste C'i +38 h .t\CS.seek 0ml C'>'4 +506 h '4 C':& h &W C'/% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'w'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'% h %0 .t\CS.seek 5ml C'|'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'n'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&5& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&E' +90 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'J( +416 h ( M& & h &ӷ M&% h % .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&71 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:40:47CEST30-Aug-18\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:40:42CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*]% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C')& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(!. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +6 F*6% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *y' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&H& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &R( +166 h ( M&a& h & A(x& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &j'н +89 h 'н A(& h &~ .t\SE.seek 6.71 Pucks below Top Plate A(' +196 h ' A(K& h &~ M&U& h & .t\Elbow.seek 3 M&q( +6 h ( M&B& h & A(Q& h &~ .t\Forearm.seek 3 + 300 counts M&v'- +23 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&S& h & M&+% h %- .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.seek CC M&e(ӷ +104 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'W(W +416 h (W .tHand.open M&r1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)I% h % C's& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 S)@% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S) +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'$9 h 9Td +100 C'm9 h 9kd +100 C'f9 h 9d +100 C' 9 h 9d +100 C'L9 h 9d +100 C'V9 h 9d +100 C'O9 h 9d +100 C''9 h 9d +100 C'-9 h 9 d +100 C'p9 h 9 d +100 C't9 h 96d +100 C'b9 h 9Ld +100 C':9 h 9bd +100 C'/9 h 9xd +100 C'99 h 9d +100 C'>9 h 9d +100 C'@9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'w9 h 9d +100 C'9 h 9d +100 C' 9 h 9(d +100 C'59 h 9>d +100 C'|9 h 9Td +100 C'^9 h 9id +100 C'i9 h 9d +100 C'"9 h 9d +100 C'*9 h 9d +100 C'09 h 9d +100 C'G9 h 9d +100 C'C9 h 9d +100 C'9 h 9  d +100 C'{9 h 9 d +100 C'89 h 9 *d +100 C'E9 h 9 ?d +100 C'N9 h 9 Td +100 C'79 h 9 j d +100 C' 9 h 9 $d +100 C'9 h 9 (d +100 C'g9 h 9 ,d +100 C']9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'!9 h 9 ( +416 h ( A(@& h &  A(& h &  M&& h &ӷ M&w% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +142 h ( M&5& h & A(|& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &^' +93 h ' .t\Elbow.hold M&i, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &"1 +150 h 1 M&*% h % A(0& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &G'; +21 h '; M&C& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &{' +22 h ' A(8% h % ."downElevator"\SE.seek :down A(E'X +100 (N% h %v M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'] ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&!1 +2 h 1 M&6& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&c(- +166 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&='η +91 h 'η .tHand.close M&)/ +150 h / M&a& h &- M&x% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' +90 h ' .t\CC.seek :closed C'Y(W +416 h (W C'K& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%Q +151 h P%S% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%R' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%j  +38 h  .t\PS.seek 0ml P%+' +1597 h ' .t\PSV.dial PSR P%, +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%<' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%v  C'  h +75 .t\PSV.dial :mixloop P%q +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%[  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.seek 2.5ml P%J' +2609 h ' .t\PSV.dial :mixloop P%I +38 h .t\PS.seek 0ml P%s' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%z +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.seek CC M&y' +91 h ' .tHand.close M&3/ +150 h / .t\CC.seek :open C'X( +416 h ( M&F& h &ӷ M&.% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\\'η +91 h 'η .tHand.open M&~1 +150 h 1 M&#% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M& % h %\  A(% h %X A(d. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (&(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &~ M&\ & h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &P( +166 h ( M&l& h & A(1& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &e'н +90 h 'н A(& h &~ .t\SE.seek 5.71 Pucks below Top Plate A(_' +205 h ' A(?& h &~ M&$& h & .t\Elbow.seek 3 M&m( +6 h ( M&& h & A( & h &~ .t\Forearm.seek 3 + 300 counts M&L'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &'/ +150 h / ."downElevator"\SE.seek :down M&-& A(h'X h & M&o% h %- .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M&((ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &f' +91 h ' .t\CC.seek :closed C'n(W +416 h (W .tHand.open M&V1 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'p  +150 h  .t\CSV.dial CTV C'W  +19 h  .t\CS.seek 5ml C'@'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%w  +38 h  .t\PS.seek 2.5ml P%5' +2610 h ' .t\PSV.dial :mixloop P%| +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%t +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%"  +150 h  .t\PS.seek :empty P%*' +2610 h ' .t\CBV.dial :waste1 C'0 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'C  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'8  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +18 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'N' +2647 h ' .t\CSV.dial CTV C'7  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'] @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&!% h %\  A(6% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (^( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (G& h &  M&O& h &ӷ M&{% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &H( +142 h ( M&& h & A(E& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &2' +90 h ' A(k& h &  .t\SE.seek 13.71 Pucks below Top Plate A(i'n +125 h 'n M&)& h & A(a& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &x' +25 h ' .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(=- h -+n .t\SE.seek 14.04 Pucks below Top Plate A(' +27 h ' M&U% h % A(c& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'; +21 h '; M&>& h & A(K& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(' +36 h ' M&Q& h & M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +90 h '\  .t\Elbow.seek CC M&j(ӷ +142 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'( +416 h ( A(q& h &  A([& h &  M&<& h &ӷ M&r% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &I( +143 h ( M&J& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &s' +92 h ' .t\Elbow.hold M&z, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&`'; +21 h '; M&3& h & A(D& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(Y% h % ."downElevator"\SE.seek :down A(F'X +100 (.% h %- M&y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%%  +113 h  P%% h % .t\PS.seek 0.499ml P%~' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%# +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +18 h  .t\PRV.dial :air P%d  +19 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %\ ' +251 h ' .t\PRV.dial :waste2 P%} @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %\\' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'l& h & .tHand.open M&11 +2 h 1 M&& h & M&T% h %\  .t\Elbow.seek FlushPuck::Garage M&?(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&'& h &- M&-% h %η .t\Forearm.seek :retract M&o'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &:' +91 h ' .t\CC.seek :closed C'L(W +416 h (W C'_& h &W .tHand.open M&m1 +150 h 1 M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'X& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%V @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%4  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%p  +76 h  .tdelay 2 seconds +200 P%W% h % .t\PS.seek 1.499ml P%f' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%&' +1094 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%@' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%w  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %|'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%t% h %r .t\PS.seek 0.475ml P%"' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%5  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%0'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%C  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %8' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%7  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%] +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%!' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%6 +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %O' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'{ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %E'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'k { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %(' +1312 h ' .t\PSV.dial :air P%)  +38 h  .t\PS.seek 2.5ml P%a' +2610 h ' .t\PSV.dial :PML P%= +37 h .t\PS.seek 0ml P%U' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'c& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'>& F*K @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*9  .t\AIV.dial :bypass .\PSV.dial PSR P%Q +22 h  ."MFBPCR"delay 20 seconds +91 h P%S% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*H I +129 h I F*i% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *x'6 +66 .t\PSV.dial :waste1 P%R  +38 h  .t\PS.seek 0ml P%,' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*v  +22 h ' .t\PSV.dial PSR P%B +21 h  F*n% h %6 ."MFBPCR"\AS.seek 0.329ml F*q'ͯ +92 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%<' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*r % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%+' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*j  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%3' +217 ."MFBPCR"\ATV.dial :AIV F*D I +43 h I F*Y% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%I +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial PSR P%y +113 h P%s% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%`' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*F% h % ."MFBPCR"\AS.seek 0.041ml F*^'% +336 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%#' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%d' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +37 h  .t\PS.seek 0ml P%~' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%} +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%\ ' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%1 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%T  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %$' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*; I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *z'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%'' +2610 h ' .t\PSV.dial :mixloop P%- +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %M' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %e' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*b  +43 h  F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *o'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*_% h %Q ."MFBPCR"\AS.seek 0.01ml F*m' +170 h ' .t\PSV.dial :mixloop P%h +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %X' +619 h ' ."MFBPCR"\ATV.dial :waste F*V n +128 h n ."MFBPCR"\AS.seek :empty F*:'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*4  +86 h  F*p% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *W' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*\\  +43 h F*f% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'&  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  '/  +38 h  +113  .t\CBV.dial :waste1 C't @ +38 h @ .t\CS.seek 0ml C'|'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*5  +22 h ."MFBPCR"delay 3 seconds +300 F*"% h %_ ."MFBPCR"\AS.seek 0.434ml F*C'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*8% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*7'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*]% h %& ."MFBPCR"\AS.seek 0.092ml F*N'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'6'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&O' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'E( +416 h ( M&)& h &ӷ M&(% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &a(- +264 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &9'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&S% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'H& h & M&i% h %\  A(R% h %X A(Z. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (2(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&,& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&v& h & A(n& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &B'н +89 h 'н A([& h &~ ."WCR"\SE.seek 4.71 Pucks below Top Plate A(x' +215 h ' A( & h &~ M&r& h & ."WCR"\Elbow.seek 3 M&q( +6 h ( M&& h & A(j& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&'- +23 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F*Q% h % k ."MFBPCR"\AS.seek 0.779ml F*S'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*= I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *H' +4416 h ' ."MFBPCR"\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.seek :empty F*R'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*Z % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *,' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*9% h % ."MFBPCR"\AS.seek 0.579ml F*v' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %  ."MFBPCR"\AS.seek 0.604ml F*a'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*j  +43 h  ."MFBPCR"\AS.seek 0ml F*<'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *D'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*B% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*3 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*y% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*x  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*q I +85 h I F*J% h % ."MFBPCR"\AS.seek 0.12ml F*N' +626 h ' ."MFBPCR"\ATV.dial :air3 F*Y % +21 h % F*E% h % ."MFBPCR"\AS.seek 0.13ml F*`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F**' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*2% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *d'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*# @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*^ I +86 h I ."MFBPCR"\AS.seek 0ml F*1'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*T  +43 h  #"MFBPCR"\Swish #1 of 2 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *;'\  +1032 h '\  F*l% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*$% h %(A ."MFBPCR"\AS.seek 0.6ml F*-'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*M'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*b% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *?' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*z % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0709h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*_  +64 h  ."MFBPCR"\ARV.dial :carrier F*h  +10 h  F*\ % h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*W  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*w  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*t % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*/'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +21 h n ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*8  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 F*7  +65 h ."MFBPCR"\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*G % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254679 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'{  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'P @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'L  +94 h  C')% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'O' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'(  'c  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '!'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'Q  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '>' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'=  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'S  +38 h  C'i% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'Z' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'H @ +76 h @ +50 S)R% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'9% h % ."WCR"\CSV.dial between air and CTV C', ` +56 h ` ."WCR"\CS.seek 7.288ml C'n' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  'v  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'U'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'a' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +37 h  C'r% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'<' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)B% h % ."WCR"\CBV.dial between CSV and waste2b C'D  +94 h  C'3% h % ."WCR"\CSV.dial between air and CTV C'y ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'x  ."WCR"\CSV.dial CTV C'q  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'J'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Y' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'E  +151 h C'N& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&% h %\  A(% h %X A(`. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (I& h &  M&*& h &ӷ M&s% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &2( +143 h ( M&& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  ."WCR"\SE.seek 14.71 Pucks below Top Plate A(A'] +116 h '] M&& h & A(d& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &^' +25 h ' ."WCR"\Elbow.hold M&~, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(1- M&$&.,(\ #b h -+] ."WCR"\SE.seek 15.04 Pucks below Top Plate A(T' +27 h ' M&}% h % A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&l'; +21 h '; M&;& h & A($& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +22 h ' ."WCR"\SE.seek 16.04 Pucks below Top Plate A(' +37 h ' M&-& h & M&b% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +89 h '\  ."WCR"\Elbow.seek CC M&z(ӷ +142 h (ӷ M&?& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&_' +91 h ' ."WCR"Hand.close M&h/ +150 h / ."WCR"\CC.seek :open C'\ ( +416 h ( A('& h &  A(e& h &  M&m& h &ӷ M&V% h % ."WCR"\Forearm.seek :retract M&4'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&\\& h & A(f& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &p' +92 h ' ."WCR"\Elbow.hold M&W, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&o% h % A(w& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&@'; +21 h '; M&&& h & A(t& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A("'X +100 (% h %X M&/% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'|& h & .tHand.open M&01 +2 h 1 M&8& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +165 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.seek :garage M&]'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'L& h &W .tHand.open M&)1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'c& h &W C'(% h % .t\CSV.dial CSR C'O  +37 h  C'Q% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'!'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '=% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'S'[ +463 h '[ .t\CSV.dial :waste C'i +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '>'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'Z& h &W C'H% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C',% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  '[  +37 h  +113  .t\CBV.dial :waste1 C'n @ +76 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'U& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C'r'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'<% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'B  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'D  +76 h  .t\CS.seek 0ml C'3'4 +766 h '4 #t\Flushing upper manifold C'y& h &W C'% h %4 .t\CSV.dial :flush C'q  +75 h  .t\CS.seek 5ml C'x'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'J'i +416 h 'i ."connect"\CSV.dial CBV C'E .t\CBV.dial CSV C'N  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'`'0 +507 h '0 .t\CSV.dial :waste C'I +37 h .t\CS.seek 0ml C'*'4 +507 h '4 C's& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +38 h @ C'% h %0 .t\CS.seek 5ml C'd'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'}'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C';'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'$& h &W C'F% h %4 .t\CSV.dial CSR C'-  +150 h  C'b% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'2' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A +113 h .t\CTV.dial :intake C'^  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'?' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'T  +113 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  'M  +37 h  +113  .t\CBV.dial :waste1 C'' @ +38 h @ .t\CS.seek 0ml C'e'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'm  +37 h  C'V% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  +37 h  .t\CBV.dial :waste2b C'z  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'o  +38 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '&'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '4  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C'| +38 h .t\CS.seek 0ml C'0'4 +506 h '4 #t\Flushing upper manifold C'8& h &W C' % h %4 .t\CSV.dial :flush C'p  +38 h  .t\CS.seek 5ml C'@'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'C% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'"'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'%'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'5  +38 h  .t\CS.seek 3ml C']'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'6% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'G'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +506 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C')'4 +507 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'c% h %4 .t\CSV.dial :flush C'(  +38 h  .t\CS.seek 5ml C'O'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +38 h @ C'P% h %p .t\CS.seek 7ml C'Y'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  '!  +38 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C'S'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'Z& h &W C'H% h %4 .t\CSV.dial :flush C'R  +75 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C',% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  +37 h  .t\CBV.dial :waste2b C'[  +76 h  .t\CS.seek 0ml C'n'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'U% h %4 .t\CSV.dial CSR C'  +37 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C' 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'a' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'< 'r  +38 h +75 .t\CTV.dial :intake C'B  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'D' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'y'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'3'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1373 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'x'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&N& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&I/ +150 h / .t\CC.seek :open C'`( +416 h ( M&s& h &ӷ M&% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &*(- +265 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&d1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'}& h & .tHand.open M&$1 +2 h 1 M&F& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &-(- +7 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&A/ +150 h / M&^& h &- M&2% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +264 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'~(W +416 h (W C'.& h &W .tHand.open M&M1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'm& h &W C'V% h %4 .t\CSV.dial CSR C'e  +113 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'_'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'z% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C'o +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'w'4 +2010 h '4 #t\Purging external Intake C'& h &W C'&% h %4 .t\CSV.dial :flush C'4  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C't  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'|  +76 h #t\Flushing upper manifold C'/& h &W C'8% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV C'@ .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'" +38 h .t\CS.seek 0ml C'W'4 +506 h '4 C'%& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'5'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'6'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C']'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'G +75 h .t\CS.seek 0ml C'L'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&\ & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.seek CC M&)' +90 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'P( +416 h ( M&O& h &ӷ M&=% h % .t\Forearm.seek :retract M&!'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &i(- +265 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &H'η +91 h 'η .tHand.open M&R1 +150 h 1 M&S% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Completed [mbaPhase] begun 06:03:05 #\Preparing to sleep until Tue Oct 09 06:03:05 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:03CEST09-Oct-18 #\Continuing at 06:03CEST09-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeploy.rb:120\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:42:13CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:03CEST09-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0709h25ml.pcr F*,% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*D  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *q'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*J  +32 h  ."MFBPCR"\ATV.dial :air3 F*I % +86 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*T  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*` n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +21 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *e'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*\\  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*x' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*z  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*o' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*f  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*4  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*t n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*|'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*0  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0711h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*7  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +21 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*]'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +21 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*(  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*K'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*v'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*y% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*I  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*{  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*s I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*+ n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*d  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*^' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F**  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0713h25ml.pcr F*-% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*M% h %Z ."MFBPCR"\AS.seek 0.81ml F*\\'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*x% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +64 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*%% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*O n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Z  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*! n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*S' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*, I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*K' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*[ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*v'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*U  +107 h  ."MFBPCR"delay 7 seconds +700 F*>% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*n% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*B' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*D% h % \\ ."MFBPCR"\AS.seek 0.326ml F*a'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0716h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *I'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*J'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*d  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*E% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +32 h  ."MFBPCR"\ATV.dial :air3 F*; % +86 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ARV.dial :carrier F*f  +10 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*|  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*#  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*C' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*7  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*5'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0718h25ml.pcr F*(% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*O  +22 h ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*R% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*[  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*<  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*3  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*;  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*`  +22 h  ."MFBPCR"delay 7 seconds +700 F*'% h %< ."MFBPCR"\AS.seek 0.594ml F*V'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +21 h n ."MFBPCR"delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*_' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*x  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*4'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*o @@T ."MFBPCR"\ASV.dial :SPEdelivery F*&  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*t  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *|' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*0  +43 h  F*/% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*@ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *#'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *%' +424 h ' ."MFBPCR"\ATV.dial :air3 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*p% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*W% h % H ."MFBPCR"\AS.seek 0.735ml F*6' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*5% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*] n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*c% h % zG ."MFBPCR"\AS.seek 0.365ml F*G' +7161 h ' ."MFBPCR"\ATV.dial :waste F*\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*( % +43 h % F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*=% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %  ."MFBPCR"\AS.seek 0.604ml F*Z'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F*H% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*)' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*, n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*Y'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*K % +107 h % F*S% h %V ."MFBPCR"\AS.seek 0.01ml F*U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*v% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*<'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*D n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*B  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*q  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *a'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*3 n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *N'R +202 h 'R ."MFBPCR"\SPE.coast F*{  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*I n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*s  +43 h  F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *J' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*d  +43 h  F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*1  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *}'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*F n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *b' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*A I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *^': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *-'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F** n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *.' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *T': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*M n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *m'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*V n +150 h n ."MFBPCR"\AS.seek 0ml F*e'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*\\  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*l % +43 h % F*x% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*_% h % ."MFBPCR"\AS.seek 0.96ml F*f'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %0 ."MFBPCR"\AS.seek 0.985ml F*4' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b */'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *8'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*#  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *7' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9987 h ' ."MFBPCR"\ATV.dial AIV F*C I +43 h I ."MFBPCR"\AS.seek 0.02ml F*%' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*p'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*"% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*W  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *5'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*]% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +21 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*G'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*?'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*~  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*=  +65 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*) % +87 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*S% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h %> ."MFBPCR"\AS.seek 0.835ml F*U'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *r'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V @CEST1538949617.95 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *a'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*N % +108 h % ."MFBPCR"\AS.seek 0ml F*I'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast M&5 P%6 +2 %5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539057785.33 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )s h  C' 'k h  P% % h  F* *+ h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ C' 2\ F*\ 9\ S)&\ .t\SPE.coast F*\ 9\ *\ 9\ *J  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)d% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'E& S)#.0X 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:43:10CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #14) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&41 +150 h 1 M&& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &0(- +166 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &@'η +91 h 'η .tHand.close M& / +150 h / M&#& h &- M&% h %η .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +264 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'"(W +416 h (W C'%& h &W .tHand.open M&p1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'6  +150 h  C']% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '5'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '\ '[ +463 h '[ .t\CSV.dial :waste C'c +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '('4 +2009 h '4 #t\Purging external Intake C'Q& h &W C'G% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O '?  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'R'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'h  +75 h #t\Flushing upper manifold C'!& h &W C'H% h %4 .t\CSV.dial :flush C'Z  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C') @ +37 h @ C'Y% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CBV C'K .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'S'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +507 h '0 .t\CSV.dial :waste C'> +37 h .t\CS.seek 0ml C'v'4 +507 h '4 C'& h &W C'n% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'U'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'r'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'<'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'B +75 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'{( +416 h ( M&3& h &ӷ M&N% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &X(- +264 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &u'η +91 h 'η .tHand.open M&:1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:43:15CEST30-Aug-18\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:43:10CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*I% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'o& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&s% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(k% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (+(~ +6 F*J% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *d' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (E& h &~ M&& h &- M&1% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&& h & A(F& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & 'н +90 h 'н A(}& h &~ .t\SE.seek 3.71 Pucks below Top Plate A(A' +223 h ' A(b& h &~ M&;& h & .t\Elbow.seek 3 M&( +7 h ( M&$& h & A(& h &~ .t\Forearm.seek 3 + 300 counts M&2'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&-% h %- .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.seek CC M&V(ӷ +133 h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +90 h ' .t\CC.seek :closed C'w(W +416 h (W .tHand.open M&&1 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'l& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)e% C''4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  h  ."readyMFB"\ASV.dial :waste1 F*[  h +194 ' +586 .t\SSV.dial :spit,avoiding: :exhaust S) +150 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'n  .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'09 h 9Td +100 C'89 h 9kd +100 C't9 h 9d +100 C'%9 h 9d +100 C'p9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'69 h 9 d +100 C'9 h 96d +100 C'9 h 9Ld +100 C'#9 h 9bd +100 C'g9 h 9xd +100 C'59 h 9d +100 C'c9 h 9d +100 C']9 h 9d +100 C'\ 9 h 9d +100 C'G9 h 9d +100 C'~9 h 9d +100 C'?9 h 9d +100 C'Q9 h 9(d +100 C' 9 h 9>d +100 C'W9 h 9Td +100 C'O9 h 9id +100 C'(9 h 9d +100 C'=9 h 9d +100 C'!9 h 9d +100 C'H9 h 9d +100 C'R9 h 9d +100 C'Y9 h 9d +100 C'h9 h 9  d +100 C')9 h 9 d +100 C'9 h 9 *d +100 C',9 h 9 ?d +100 C'Z9 h 9 Td +100 C'K9 h 9 j d +100 C'>9 h 9 $d +100 C'[9 h 9 (d +100 C'9 h 9 ,d +100 C'n9 h 9 0d +100 C'v9 h 9 4d +100 C' 9 h 9 8d +100 C'9 h 9 '\ +89 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'[ @ +19 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'n ` +19 h ` .t\AIV.dial :bypass C'v { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&U& h & M&r% h %\  .t\Elbow.seek FlushPuck::Garage M&S(- +166 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &N' +91 h ' .t\CC.seek :closed C'i(W +416 h (W C'"& h &W .tHand.open M&y1 +150 h 1 M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%:% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P%L' +1597 h ' .t\PSV.dial PSR P%s +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +2610 h ' ."connect"\PRV.dial CBV P%k  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%E  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%1  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%\\ +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P%b +37 h .t\PS.seek 0ml P%P' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&X& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&*' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'$( +415 h ( M&& h &ӷ M& % h % .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &-(- +265 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&;% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'{& h & M&&% h %\  A(^% h %X A(l. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (e(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (z& h &~ M&j& h &- M&+% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +166 h ( M&/& h & A(|& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &V'н +89 h 'н A( & h &~ .t\SE.seek 2.71 Pucks below Top Plate A(d') +235 h ') A(@& h &~ M&0& h & .t\Elbow.seek 3 M&8( +7 h ( M&%& h & A(p& h &~ .t\Forearm.seek 3 + 300 counts M&T'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&M% h %- .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.seek CC M&4(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' + h 'X ."downElevator"\SE.coast A(5 +90 h ' .t\CC.seek :closed C'C(W +416 h (W .tHand.open M&1 +150 h 1 M&_% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'6  +151 h  .t\CSV.dial CTV C'w  +18 h  .t\CS.seek 5ml C']'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'\ ' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'G'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P% ' +2609 h ' .t\PSV.dial :mixloop P%W +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%=  +151 h  .t\PS.seek :empty P%!' +2609 h ' .t\CBV.dial :waste1 C'H @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'Y  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'h @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'? ` +19 h ` .t\CSV.dial :air C'Q @ +38 h @ .t\CS.seek 10ml C'Z' +2647 h ' .t\CSV.dial CTV C'K  +37 h  .t\CS.seek 0ml C'['4 +2648 h '4 .t\CBV.dial :waste1 C'n @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'v  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&% h %\  A(U% h %X A(r. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (O( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (R& h &  M&& h &ӷ M&,% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &)( +143 h ( M&B& h & A(D& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A('& h &  .t\SE.seek 16.71 Pucks below Top Plate A(('; +96 h '; M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&>, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+; .t\SE.seek 17.04 Pucks below Top Plate A(' +27 h ' M&3% h % A(S& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&7'; +21 h '; M&c& h & A("& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +22 h ' .t\SE.seek 18.04 Pucks below Top Plate A(<' +36 h ' M&& h & M&:% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +90 h '\  .t\Elbow.seek CC M&I(ӷ +142 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'( +416 h ( A(J& h &  A(E& h &  M&o& h &ӷ M&1% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +142 h ( M&& h & A(u& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&b, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&X% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &m'; +21 h '; M&& h & A(q& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &9' +22 h ' A(i% h % ."downElevator"\SE.seek :down A('X +83 h 'X ."downElevator"\SE.coast A(5 +17 ( % h %X M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +89 h '\  .t\SC.coast .\PSV.dial :air A(6 P%A  +113 h  P%F% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%; +37 h .t\PRV.dial :air P%.  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%{  +38 h .t\CBV.dial AIV C'&  +37 h .t\PRV.dial between CBV and air P%^  +19 h  .t\PRV.dial :air P%l  +19 h  P%z% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %j' +250 h ' .t\PRV.dial :waste2 P%+ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&|1 +2 h 1 M& & h & M&-% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&p/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +265 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &#' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'd& h &W .tHand.open M&81 +150 h 1 M&x% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  C'$& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%_  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%6  +75 h  .tdelay 2 seconds +200 P%w% h % .t\PS.seek 1.499ml P%t' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%e' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%]' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%\ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%~  +37 h  .tdelay 2 seconds +200 P%P% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %W'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%g  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%=' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%H'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%Y  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%h  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%? +76 h .t\PTV.dial :PRV,avoiding: :puck P%Q @ +75 h @ .t\PS.seek 0ml P%G' +2610 h ' .t\PSV.dial :air P%K  +75 h  .t\PS.seek 2.5ml P%Z' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%n +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%v' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%U +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%r  +76 h P%R% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C', & +50 h & .t\AWV.select :waste C' +6 P%B% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %D'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %4' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%3' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'S& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'c& h &W F*" @@T ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*5  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%:% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*) I +129 h I F*(% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%a  +37 h  .t\PS.seek 0ml P%s' +1597 h ' .t\PSV.dial PSR P%7 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%>' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*k  +22 h ' .t\PSV.dial PSR P%N +21 h  F*C% h %6 ."MFBPCR"\AS.seek 0.329ml F*J'ͯ +92 h P%E% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%o' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*1 % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%L' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*I  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *X' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +43 h I F*i% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P%* +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%<  +38 h  .t\PS.seek 0ml P%A' +739 h ' ."MFBPCR"delay 5 seconds +500 F*F9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*9% h % ."MFBPCR"\AS.seek 0.041ml F*O'% +336 h ' .t\PSV.dial PSR P%! +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%;' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%. +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%{' +2610 h ' .t\PSV.dial PSR P%& +113 h P%^% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%l' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%z  +37 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%+ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%j' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%| +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%-  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %@' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*0 I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *b'V +849 h ' .t\PSV.dial :air P%p  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %2' +5107 h ' .t\PSV.dial :air P%%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %V' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *M'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*d% h %Q ."MFBPCR"\AS.seek 0.01ml F*8' +171 h ' .t\PSV.dial :mixloop P%x +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %$' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*#'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*_  +86 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *w' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*t% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'e  +38 h  C'\ % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'P' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  'g  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'W'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*=% h %_ ."MFBPCR"\AS.seek 0.434ml F*Y'ݏ +109 h '4 .t\CSV.dial CSR C'h  +38 h  C'H% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'?'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*Q  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*K'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*G n +108 h n F*n% h %& ."MFBPCR"\AS.seek 0.092ml F*Z'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'U'4 +2647 h '4 C'r& h &W #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&'/ +150 h / .t\CC.seek :open C'D( +416 h ( M&& h &ӷ M&4% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&S& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&1 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C')& h & M&(% h %\  A(a% h %X A(`. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (7& h &~ M&s& h &- M&y% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&k& h & A(C& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &N'н +89 h 'н A(E& h &~ ."WCR"\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': A(>& h &~ M&1& h & ."WCR"\Elbow.seek 3 M&J( +7 h ( M&u& h & A(I& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&}'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &o/ +10 h '&0 ."MFBPCR"delay 3 seconds +140 h / ."downElevator"\SE.seek :down M&& A('X h & M&q% h %- ."WCR"\Forearm.seek :retract M&i'\ +91 h '\  ."WCR"\Elbow.seek CC M&L(ӷ +69 ."MFBPCR"\ATV.dial :waste2 F*X % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +30 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' ."WCR"\CC.seek :closed C' (W +416 h (W ."WCR"Hand.open M&<1 +23 h 'R ."MFBPCR"\ATV.dial :air2 F*m  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *F' +105 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)!% h % C'A& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'.  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*1' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*u n +86 h n ."MFBPCR"\ASV.dial :wasteR F*I  +42 h  ."MFBPCR"\AS.seek 0ml F*o'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*N% h %V ."MFBPCR"\AS.seek 0.59ml F*X' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F**% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10076 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*}  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*J I +86 h I F*Z% h % ."MFBPCR"\AS.seek 0.12ml F*i' +626 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*D% h % ."MFBPCR"\AS.seek 0.13ml F*m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*!' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h % ."MFBPCR"\AS.seek 0.255ml F*.'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*&  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *l'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*; @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*O I +86 h I ."MFBPCR"\AS.seek 0ml F*|'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*F'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*-  +43 h  #"MFBPCR"\Swish #1 of 2 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *0'\  +1032 h '\  F*/% h %\  ."MFBPCR"\AS.seek 0.5ml F*p'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*@% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*2'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*b % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3473 h 'V ."MFBPCR"\SPE.coast F*%  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0907h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*d  +64 h  ."MFBPCR"\ARV.dial :carrier F*x  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*V'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *M'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*w  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*e' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*~  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*[' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*=  +107 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 F*K  +65 h ."MFBPCR"\ARV.dial :air1 F*n  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*v% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*R % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254709 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C',  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'r @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)B% h % ."WCR"\CBV.dial between CSV and waste2b C'T  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'S  '4  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'c' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C':  +38 h  C'(% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'`' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C') @ +75 h @ +50 S)a% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'5% h % ."WCR"\CSV.dial between air and CTV C's ` +57 h ` ."WCR"\CS.seek 7.288ml C'C' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'k  'E  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '3'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'>  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'u  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'I  +38 h  C'1% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'o' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)N% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'* ` +57 h ` ."WCR"\CS.seek 7.288ml C'X' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'}  'J  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Z'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'D  +150 h C'i& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&<% h %\  A(% h %X A(m. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (A( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (\\& h &  M&!& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&.& h & A(^& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(& h &  ."WCR"\SE.seek 17.71 Pucks below Top Plate A(z'* +86 h '* M&{& h & A(l& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &O' +25 h ' ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(|- M&$&.,(\ #b h -+* ."WCR"\SE.seek 18.04 Pucks below Top Plate A(-' +27 h ' M&+% h % A(F& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&/'; +21 h '; M&0& h & A(@& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &p' +22 h ' ."WCR"\SE.seek 19.04 Pucks below Top Plate A( ' +36 h ' M&& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +90 h '\  ."WCR"\Elbow.seek CC M&b(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ ."WCR"\Forearm.seek CC M&d' +91 h ' ."WCR"Hand.close M&x/ +150 h / ."WCR"\CC.seek :open C'j( +416 h ( A(& h &  A(V& h &  M&8& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&_'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&& h & A(t& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &6' +93 h ' ."WCR"\Elbow.hold M&w, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\ 1 +150 h 1 M&M% h % A(~& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &]'; +21 h '; M&e& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &P' +22 h ' A([% h % ."downElevator"\SE.seek :down A(='X +74 h 'X ."downElevator"\SE.coast A(5 +26 (h% h %X M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&H1 +2 h 1 M&& h & M&Q% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&v/ +150 h / M&G& h &- M&?% h %η .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'B(W +416 h (W C'T& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'4& h &W C'S% h % .t\CSV.dial CSR C'  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ':'[ +463 h '[ .t\CSV.dial :waste C'( +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'c'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'`& h &W C')% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'y'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C's% h %p .t\CS.seek 7ml C'7'i +415 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV C'k  +38 h  +113  .t\CBV.dial :waste1 C'C @ +75 h @ .t\CS.seek 0ml C'>'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'3& h &W C'u% h %4 .t\CSV.dial :flush C'I  +76 h  .t\CS.seek 5ml C'1'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'o% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  +38 h  .t\CBV.dial :waste2b C'q  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'*& h &W C'% h %4 .t\CSV.dial :flush C'}  +76 h  .t\CS.seek 5ml C'J'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D 'i  +37 h +75 .t\CTV.dial :air C'<  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'm'0 +507 h '0 .t\CSV.dial :waste C'\\ +37 h .t\CS.seek 0ml C'!'4 +507 h '4 C'& h &W C'.% h %4 .t\CSV.dial :flush C'^  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'{% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'|'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +506 h '0 .t\CSV.dial :waste C'F +76 h .t\CS.seek 0ml C'0'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'@& h &W C'9% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'&'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +415 h ' ."connect"\CSV.dial CBV C'z .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'O  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'%  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '/'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'-  +113 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '2  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'V'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'8  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  +38 h  .t\CBV.dial :waste2b C'b  +75 h  .t\CS.seek 0ml C'\ '4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'M  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'_  '[ +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'h'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'W +37 h .t\CS.seek 0ml C'H'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'Q% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.seek 5ml C']'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'Y% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'= +112 h .t\CTV.dial :air .\CS.seek 0ml C'P'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +507 h '0 .t\CSV.dial :waste C'w +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'G& h &W C'?% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'n'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'U% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'R'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'4% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'( @ +37 h @ .t\CS.seek 0ml C':'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'`& h &W C')% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C's% h %p .t\CS.seek 7ml C'y'i +416 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'k  +76 h  .t\CS.seek 0ml C'C'4 +765 h '4 #tDisinfecting external Intake with kill C'7& h &W C'3% h %4 .t\CSV.dial CSR C'u  +38 h  C'I% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'>'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'o '1  +37 h +76 .t\CTV.dial :intake C'N  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '*'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'}% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'X' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'J'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C'm( +416 h ( M&& h &ӷ M&.% h % .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &!(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&l1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&@1 +2 h 1 M&9& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.seek :garage M&&'η +91 h 'η .tHand.close M&z/ +150 h / M&O& h &- M&% h %η .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&21 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'8& h &W C'% h %4 .t\CSV.dial CSR C'V  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'b% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +462 h '[ .t\CSV.dial :waste C'M +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +2010 h '4 #t\Purging external Intake C'\ & h &W C'e% h %4 .t\CSV.dial :flush C'_  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'h'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'W  +75 h #t\Flushing upper manifold C'g& h &W C'% h %4 .t\CSV.dial :flush C'Q  +76 h  .t\CS.seek 5ml C'6'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C' % h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'='4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C'P +38 h .t\CS.seek 0ml C'w'4 +506 h '4 C'A& h &W C'G% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.seek 3ml C' '0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +38 h @ C'r% h %0 .t\CS.seek 5ml C'U'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'n'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'R +75 h .t\CS.seek 0ml C'T'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &((- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &)'η +91 h 'η .tHand.open M&a1 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Completed [coreNEG] begun 06:03:20 #\Preparing to sleep until Thu Oct 11 06:03:20 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:03CEST11-Oct-18 #\Continuing at 06:03CEST11-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:44:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:03CEST11-Oct-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0907h.pcr F*s% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*E  +21 h ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *7'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*I'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*.'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*l% h %m ."MFBPCR"\AS.seek 0.82ml F*|'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0909h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ARV.dial :carrier F*-  +10 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *V'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*/% h %Fj ."MFBPCR"\AS.seek 0.524ml F*J' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*b  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*M' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*t  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +107 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*h  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*H  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0909h.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*K  +22 h ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +21 h F*v% h %Z ."MFBPCR"\AS.seek 0.81ml F*r'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*n'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*a% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*)% h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*E'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*3  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0912h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*}  +11 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\\  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +21 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*L n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*D' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*l  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*^'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +108 h  ."MFBPCR"delay 7 seconds +700 F*+% h %< ."MFBPCR"\AS.seek 0.594ml F*@'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*9 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*O' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*!  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*-'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0912h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*%  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*W  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*H% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*Y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +21 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*A% h %m ."MFBPCR"\AS.seek 0.82ml F*G'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*P  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0914h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*x'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*)% h %Fj ."MFBPCR"\AS.seek 0.524ml F*:' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*s I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*k % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*E'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +107 h  ."MFBPCR"delay 7 seconds +700 F*c% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*7 n +22 h n ."MFBPCR"delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.6ml F*>'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0914h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*Z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F*B'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*l  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*D% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *|'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*z  +31 h  ."MFBPCR"\ATV.dial :air3 F*0 % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*p'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*-'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0916h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +10 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*W  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*Q n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*]% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*;  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*K  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*P  +22 h  ."MFBPCR"delay 7 seconds +700 F*G% h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +21 h n ."MFBPCR"delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*U  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*n  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*4% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0916h.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *`'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*:'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +64 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*N% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0919h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*|% h %V ."MFBPCR"\AS.seek 0.5ml F*+'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*9 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*0  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*& I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*O% h %Fj ."MFBPCR"\AS.seek 0.524ml F*!' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*F % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*p' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*%  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*-'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.6ml F*/'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*J  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*b  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*_'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0919h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*M @@T ."MFBPCR"\ASV.dial :SPEdelivery F*e  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *W' +424 h ' ."MFBPCR"\ATV.dial :ASV F*h n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*H  +43 h  F*g% h % ."MFBPCR"\AS.seek 0.62ml F*Q'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*] n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *;'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*='(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *A' +424 h ' ."MFBPCR"\ATV.dial :air3 F*Y% h % ."MFBPCR"\AS.seek 0.53ml F*P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*6% h % ."MFBPCR"\AS.seek 0.71ml F*v' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h % H ."MFBPCR"\AS.seek 0.735ml F*U' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F* % h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*n n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*4% h % zG ."MFBPCR"\AS.seek 0.365ml F*R' +7161 h ' ."MFBPCR"\ATV.dial :waste F*j n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*x' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*a% h %  ."MFBPCR"\AS.seek 0.604ml F*`'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*)% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*s n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*" % +108 h % F*:% h %V ."MFBPCR"\AS.seek 0.01ml F*3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*E% h % ."MFBPCR"\AS.seek 0.58ml F*7' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*u% h % q ."MFBPCR"\AS.seek 0.605ml F*o'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +21 h F*1% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*I' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*q n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*}  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *X' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +202 h 'R ."MFBPCR"\SPE.coast F*,  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*<'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*\\ n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *Z' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*L I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *.'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*l  +43 h  F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*|  +64 h  F*{% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *+'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*9 n +65 h n F*^% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*z I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*@  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *O': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*F% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*! n +65 h n F*%% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*p I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *-': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*2 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *8'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*V'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*/ % +43 h % F*J% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"User Abort!!:@checkpoint: omitted F*\  #t-> exit #m\EXIT @1535640326.04CEST @1535642010.53CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> delayUntil "12:00 13-sep-18" .delayUntil "12:00:00.00CEST13-Sep-18" @CEST1536832800.00 #-> execute "18tundeploy.rb" .GoodNews.suppressMail "Starting 18tundeploy w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:13:38CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeploy begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 12:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:14:19CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 12:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:14:23CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q  'R h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:14:27CEST30-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:14:23CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'Q9 h 9|d +100 C'R9 h 9d +100 C'U9 h 9d +100 C'S9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'P9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 ( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&I& ."raiseStack"\SE.seek :upperLimit h &ӷ A(E'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h & A(Q& h & M&R& h &ӷ M&U% h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(P& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %Y A(]& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&\\'υ +21 h 'υ M&^& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&&& h & A(A'X M&B% h %; .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'Q' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'R'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%P +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'S ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h & M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h & .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'Y +25 h 'Y .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %Y A(p& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &u'υ +21 h 'υ M&O& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h & A( & h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&&% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&A1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%Q +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %P'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%S +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%R' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& F*O @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%& +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*I% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'Q% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'H  .t\CTV.dial CSV C'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'P'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'S'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*R n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&q% h %; ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1315h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*Q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'x  ':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'Y +25 h 'Y ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %Y A((& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&0'υ +21 h 'υ M&3& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&&& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(2& h & A(& h & M&A& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +142 h ( M&& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Q1 +150 h 1 M&B% h %Y A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'υ +21 h 'υ M&%& h & A(F& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'P& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&R& h &- M&S% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'x% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'q  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'8% h %4 .t\CSV.dial CSR C'&  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'9' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'<  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'A  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'Q'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'B  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X 'N  +37 h +75 .t\CTV.dial :air C'\  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'P +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +507 h '4 C'R& h &W C'S% h %4 .t\CSV.dial :flush C'W  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'"'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C':% h %4 .t\CSV.dial :flush C'x  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV C'C  .t\CTV.dial CSV C'd  +37 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C' '4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&8& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&>% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'I'[ +463 h '[ .t\CSV.dial :waste C'B +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C'Q& h &W C'%% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'\ .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'P  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C'R% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'm +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Completed [mbaPhase] begun 12:00:26 #\Preparing to sleep until 06:00 15-sep-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00 15-sep-18 #\Continuing at 06:00CEST15-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeploy.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:51CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST15-Sep-18" +235134 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi !; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1315h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1317h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +107 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*P'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1317h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*^  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1319h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*/  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*('\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*4 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1319h25ml.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*<  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*B  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*P  +32 h  ."MFBPCR"\ATV.dial :air3 F*L % +86 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1322h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +10 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*d n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*| I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CEST1536876607.17 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1322h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +31 h  ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +21 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1400h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*l n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*s  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +22 h  ."MFBPCR"delay 7 seconds +700 F*R% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1400h25ml.pcr F*x% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *;' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1402h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *z'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*4 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*<  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +22 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +199 .\ARV.dial :carrier .delay 2 seconds +199 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1402h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*B @@T ."MFBPCR"\ASV.dial :SPEdelivery F*%  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*F  +151 h F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *P' +423 h ' ."MFBPCR"\ATV.dial :ASV F*L n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*M% h % ."MFBPCR"\AS.seek 0.62ml F*l'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *s'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*f' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % H ."MFBPCR"\AS.seek 0.735ml F*e' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +21 h F*W% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*:% h % zG ."MFBPCR"\AS.seek 0.365ml F*m' +7161 h ' ."MFBPCR"\ATV.dial :waste F** n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*x % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*C% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +21 h F*`% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*"' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*| n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*b'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *n'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*O % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*u' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*q n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*;'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*? n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *r'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +202 h 'R ."MFBPCR"\SPE.coast F*V  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +42 h  F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *~': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*1  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *7'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*8 n +65 h n F*$% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*! I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *k': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*z  +64 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *&'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Y n +65 h n F*<% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*2  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *A'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*J n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*0 % +43 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*)% h % ."MFBPCR"\AS.seek 0.96ml F*I'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %0 ."MFBPCR"\AS.seek 0.985ml F*N' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.97ml F*X'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*L I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *M'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Z' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*R n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*[% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*+  +21 h ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*\\% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *r'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*{ % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1536984000.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '@ h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)/% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'c& S)#.0X  ."rdySampler"\CTV.dial :air h %3 C'z  M&4% h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &k'\ h ."rdySampler"\CSV.dial :waste1 C'Y h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)< h  S)&  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %'' +20 h '\  ."closeHand"Hand.close M&/ .t\Elbow.jog 1500 M&2* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:16:47CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&N1 +150 h 1 M&L& h &_ M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &](- +166 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &H'η +91 h 'η .tHand.close M&l/ +150 h / M&s& h &- M& % h %η .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &R' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'S% h %4 .t\CSV.dial CSR C'e  +150 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C': +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'x'4 +2009 h '4 #t\Purging external Intake C'& h &W C'm% h %4 .t\CSV.dial :flush C',  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h '9  h +75 .t\CTV.dial :intake C'D  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'=  +75 h #t\Flushing upper manifold C'd& h &W C'`% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'E'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'b% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CBV C'O .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'p +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'n& h &W C'K% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'a'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'u% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&w& h &ӷ M&{% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:16:52CEST30-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:16:47CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*.% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'B& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(@% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&/% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &c( +6 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F*1 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +160 h ( M&~& h & A(8& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +89 h ' A(7& h &  .t\SE.seek 17.71 Pucks below Top Plate A(!'* +87 h '* A(G& h &  M&(& h & .t\Elbow.seek 2 M&3( +6 h ( M&& h & A(4& h &  .t\Forearm.seek 2 + 300 counts M&z'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down A(>'X M&<& h & M&&% h %; .t\Forearm.seek :retract M&'\ +87 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .t\Elbow.seek CC M&J(ӷ +143 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'U(W +416 h (W .tHand.open M&%1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'0& h &W ."airStroke"\CSV.dial :waste S)% h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors ."airStroke"\CS.seek 0ml .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust C'\ '4 .t\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9$0d +100 C'39 h 9<4d +100 C'9 h 9S8d +100 C'V9 h 9kd +100 C']9 h 9Td +100 C'_9 h 9kd +100 C'F9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C's9 h 9d +100 C'X9 h 9d +100 C'i9 h 9d +100 C'S9 h 9 d +100 C'e9 h 9 d +100 C'Z9 h 96d +100 C'R9 h 9Ld +100 C')9 h 9bd +100 C'g9 h 9xd +100 C'W9 h 9d +100 C':9 h 9d +100 C'\\9 h 9d +100 C'*9 h 9d +100 C'm9 h 9d +100 C',9 h 9d +100 C'99 h 9d +100 C'9 h 9(d +100 C'D9 h 9>d +100 C'+9 h 9Td +100 C'h9 h 9id +100 C'x9 h 9d +100 C'C9 h 9d +100 C'd9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'b9 h 9d +100 C'=9 h 9  d +100 C'"9 h 9 d +100 C'E9 h 9 *d +100 C'|9 h 9 ?d +100 C'69 h 9 Td +100 C'O9 h 9 j d +100 C'p9 h 9 $d +100 C' 9 h 9 (d +100 C'n9 h 9 ,d +100 C'K9 h 9 0d +100 C' 9 h 9 4d +100 C'9 h 9 8d +100 C'u9 h 9 'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'3 @ +38 h @ .t\CS.seek 10ml C'' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'V ` +132 h ` #tLysing Complete C'%& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(0% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (L( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&j% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&M& h & A('& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\ ' +89 h ' A(P& h & .t\SE.seek 1.71 Pucks below Top Plate A( ': +244 h ': M&l& h & A(N& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b & 'Y +26 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(H- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(' +27 h ' M&]% h %Y A(_& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&F'υ +20 h 'υ M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(X' +37 h ' M&i& h & M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +89 h '\  .t\Elbow.seek CC M&Z(ӷ +143 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C':( +415 h ( A(\\& h & A(*& h & M&m& h &ӷ M&,% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&D& h & A(+& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &h'Y +92 h 'Y .t\Elbow.hold M&x, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&d% h %Y A(`& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&b& h & A(=& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &"' +22 h ' A(E% h % ."downElevator"\SE.seek :down A(|'X +100 (6% h %1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'n' +6 h ' .t\CSV.dial between air and CTV C'K ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&u1 +2 h 1 M&a& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &{' +91 h ' .t\CC.seek :closed C'#(W +416 h (W C'[& h &W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%? +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%r' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%.  +38 h  .t\PS.seek 0ml P%-' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%B' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%@  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%o  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :mixloop P%~ +38 h .t\PS.seek 0ml P%8' +2609 h ' .t\PSV.dial :air P%1  +38 h  .t\PS.seek 2.5ml P%7' +2610 h ' .t\PSV.dial :mixloop P%( +37 h .t\PS.seek 0ml P%G' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&<' +90 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'4( +416 h ( M&c& h &ӷ M&$% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +265 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Q'η +91 h 'η .tHand.open M&31 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&%% h %\  A(k% h %X A(0. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&j% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &M( +166 h ( M&'& h & A(P& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(& h &  .t\SE.seek 16.71 Pucks below Top Plate A(l'; +96 h '; A(N& h &  M&H& h & .t\Elbow.seek 2 M&]( +6 h ( M&_& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &i/ +150 h / ."downElevator"\SE.seek :down M&\ & A(S'X h & M&% h %; .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M& (ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'e  +150 h  .t\CSV.dial CTV C'U  +19 h  .t\CS.seek 5ml C'\\'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'*' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'm'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%,  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P%+ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Z +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%C  +151 h  .t\PS.seek :empty P%d' +2609 h ' .t\CBV.dial :waste1 C'` @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'b  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'= @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'9 ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'6' +2648 h ' .t\CSV.dial CTV C'O  +37 h  .t\CS.seek 0ml C' '4 +2647 h '4 .t\CBV.dial :waste1 C'h @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'K  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(u% h %X A(a. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&X& h &ӷ M&"% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +143 h ( M&q& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &|' +89 h ' A(& h & .t\SE.seek 2.71 Pucks below Top Plate A(x') +235 h ') M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&p, +7 h , .t\Elbow.reconfigure TightElbow A(;- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(f' +27 h ' M&w% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&^'υ +21 h 'υ M&:& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(' +36 h ' M&?& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +90 h '\  .t\Elbow.seek CC M&.(ӷ +142 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(& h & M&B& h &ӷ M&o% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &~( +142 h ( M&/& h & A(1& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &8'Y +93 h 'Y .t\Elbow.hold M&(, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &71 +150 h 1 M&T% h %Y A( & h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &Y'υ +21 h 'υ M&&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' A(#% h % ."downElevator"\SE.seek :down A(c'X +100 ($% h %  M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%!  +2 A(6 +111 h  P%5% h % .t\PS.seek 0.499ml P%3' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%2  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'%  +38 h .t\PRV.dial between CBV and air P%k  +18 h  .t\PRV.dial :air P%0  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%j @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %Q' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C''& h & .tHand.open M&P1 +2 h 1 M&& h & M&A% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&>/ +150 h / M&i& h &- M&\ % h %η .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +265 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &)' +91 h ' .t\CC.seek :closed C'l(W +416 h (W C'& h &W .tHand.open M&]1 +150 h 1 M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'4& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%s @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%e  +76 h  .tdelay 2 seconds +200 P%U% h % .t\PS.seek 1.499ml P%F' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%n  +37 h  .t\PS.seek 0ml P%\\' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%* +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%,  +37 h  .tdelay 2 seconds +200 P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %+'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%g  +75 h .tdelay 2 seconds +200 P%Z% h %r .t\PS.seek 0.475ml P%C' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%D  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%`'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%b  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%=  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %E' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%9 +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%m' +2610 h ' .t\PSV.dial :air P%O  +75 h  .t\PS.seek 2.5ml P%6' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%h +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%K' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%u +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%a  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %X' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'" & +50 h & .t\AWV.select :waste C' +6 P%q% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %L'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb % ' +1312 h ' .t\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +37 h .t\PS.seek 0ml P%;' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'w& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*: @@T .t\AIV.dial :bypass F*[  .t\PSV.dial PSR P%? +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%|' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*y I +129 h I F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%r  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%^ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%f' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%p' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +22 h ' .t\PSV.dial PSR P%{ +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%B' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*o % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b */'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%1  +38 h  .t\PS.seek 0ml P%-' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*.  +22 h  F*7% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *T' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%&' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*#% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%~ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%$' +2610 h ' .t\PSV.dial PSR P%< +113 h P%8% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%!' +739 h ' ."MFBPCR"delay 5 seconds +500 F*59 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*t n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*c% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%d +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2609 h ' .t\PSV.dial PSR P%% +113 h P%k% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%0' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%3' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%j +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%A  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %H' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*_ I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *('V +849 h ' .t\PSV.dial :air P%>  +113 h  .t\PS.seek 2.5ml P%i' +2610 h ' .t\PSV.dial :mixloop P%\ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %z' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*R  +43 h  F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +170 h ' .t\PSV.dial :mixloop P%S +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %4' +618 h ' ."MFBPCR"\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.seek :empty F*)'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*e% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *U' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*Q  +43 h F*F% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *n'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'*% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\\  'g  +38 h  +113  .t\CBV.dial :waste1 C'Z @ +38 h @ .t\CS.seek 0ml C'+'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*D  +22 h ."MFBPCR"delay 3 seconds +300 F*C% h %_ ."MFBPCR"\AS.seek 0.434ml F*b'ݏ +110 h '4 .t\CSV.dial CSR C'=  +37 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'9'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*E% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*O'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*m n +107 h n F*h% h %& ."MFBPCR"\AS.seek 0.092ml F*6'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV C'K  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'u'4 +2647 h '4 C'a& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&X' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'L( +416 h ( M&W& h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &['η +91 h 'η .tHand.open M&?1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'y& h & M&x% h %\  A(r% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (|( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (^& h &  M&& h &- M&}% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &f( +166 h ( M&@& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &{' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A(p& h &  M&o& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&1& h & A(.& h &  ."WCR"\Forearm.seek 2 + 300 counts M&7'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &B/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down A( 'X M&/& h & M&% h %; ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.seek CC M&-(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&T& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +64 ."MFBPCR"\ATV.dial :waste2 F*< % +27 h ' ."WCR"\CC.seek :closed C'8(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +400 h (W ."WCR"Hand.open M&1 +150 h 1 M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*t  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *c' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)d% h % C'!& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'2  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %W ."WCR"Intake.select :close S)0 +7 )H% h %W ."WCR"\SSV.dial :exhaust S)\  +75 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*H% h %(A ."MFBPCR"\AS.seek 0.6ml F*\ '\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*z'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*R% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *N' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*( % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1507h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*S  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Q  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*U  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F**% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*,  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Z % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +107 h  ."MFBPCR"delay 7 seconds +700 F*=% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*E  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 F*O  +65 h ."MFBPCR"\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254571 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'"  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'a @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'M @ +75 h @ +50 S)q% h % ."WCR"\CBV.dial between CSV and waste2b C'l  +95 h  C'W% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'X' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'w  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'x% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'v' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S)r% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'[% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'@  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'p'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'o  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'.  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C';  +38 h  C'1% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'f @ +75 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'~% h % ."WCR"\CSV.dial between air and CTV C'7 ` +57 h ` ."WCR"\CS.seek 7.288ml C'T' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '<  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'6  +150 h C'#& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&Y% h %\  A(t% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (!( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (2& h & M&d& h &ӷ M&$% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &|( +142 h ( M&&& h & A(k& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(& h & ."WCR"\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' M&V& h & A(0& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y ."WCR"\Elbow.hold M&3, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(P- M&$&.,(\ #b h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(A' +27 h ' M&j% h %Y A(c& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &''υ +21 h 'υ M&_& h & A(H& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +37 h ' M&\ & h & M&R% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +89 h '\  ."WCR"\Elbow.seek CC M&((ӷ +142 h (ӷ M&N& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&S/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(i& h & A(J& h & M&]& h &ӷ M&s% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&Q& h & A(F& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &e'Y +92 h 'Y ."WCR"\Elbow.hold M&U, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &*1 +150 h 1 M&% h %Y A(,& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&\\'υ +21 h 'υ M&& h & A(Z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &G' +22 h ' A( % h % ."downElevator"\SE.seek :down A(C'X +100 (=% h % M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +90 h '\  +21 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&`1 +2 A(6 h 1 M&E& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&n(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&h'η +91 h 'η .tHand.close M&K/ +150 h / M&m& h &- M&9% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +265 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +91 h ' .t\CC.seek :closed C'q(W +415 h (W C'l& h &W .tHand.open M&W1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'w& h &W C' % h % .t\CSV.dial CSR C'X  +38 h  C'?% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'x +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'v& h &W C'y% h %4 .t\CSV.dial :flush C'r  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'p& h &W C'.% h %4 .t\CSV.dial :flush C';  +76 h  .t\CS.seek 5ml C'1'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'B% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'~'4 +765 h '4 #t\Flushing upper manifold C'7& h &W C'/% h %4 .t\CSV.dial :flush C'<  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6 '#  +37 h +76 .t\CTV.dial :air C'Y  +150 h .t\CS.seek 0ml C't'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'2 +37 h .t\CS.seek 0ml C'd'4 +507 h '4 C'$& h &W C'&% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'5% h %0 .t\CS.seek 5ml C'V'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'0'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C'c'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'_& h &W C'H% h %4 .t\CSV.dial CSR C'\  +151 h  C'R% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'%'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'|' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'N' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'A  +113 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'3'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +710 h ' ."connect"\CSV.dial CTV C'z  .t\CTV.dial CSV C'8  +37 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C'J'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C']  +38 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Q'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'(  +75 h  .t\CS.seek 0ml C'*'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C',% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'='4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'`'4 +507 h '4 #t\Flushing upper manifold C'E& h &W C'% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV C'G .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C'U +38 h .t\CS.seek 0ml C'K'4 +506 h '4 C'm& h &W C'9% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 3ml C'h'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'l +75 h .t\CS.seek 0ml C'W'4 +507 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'w% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'X'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C'M% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'x @ +38 h @ .t\CS.seek 0ml C''4 +765 h '4 #t\Flushing lower manifold's waste2b port C'v& h &W C'y% h %4 .t\CSV.dial :flush C'r  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'@  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'^& h &W C'p% h %4 .t\CSV.dial CSR C'.  +37 h  C';% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'o'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B '1  +37 h +76 .t\CTV.dial :intake C'{  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'f  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '7'4 +607 h '4 .t\CSV.dial CSR C'/  +112 h  C'<% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'~'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'T' +1373 h ' .t\CSV.dial CBV C' +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&#& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C'( +416 h ( M&$& h &ӷ M&&% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &d(- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&V1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'P& h & .tHand.open M&_1 +2 h 1 M&H& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &\ (- +7 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +264 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'z& h &W .tHand.open M&81 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C's% h %4 .t\CSV.dial CSR C'J  +113 h  C'Q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '(% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'F'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Purging external Intake C'*& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'Z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'='4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'+  +75 h #t\Flushing upper manifold C'g& h &W C'E% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'e'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ +113 h .t\CTV.dial :air .\CS.seek 0ml C'G'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'C +38 h .t\CS.seek 0ml C'U'4 +506 h '4 C'!& h &W C'm% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C'D'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'a% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'h'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'l'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'M( +416 h ( M&X& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &y'η +90 h 'η .tHand.open M&r1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Completed [coreNEG] begun 06:00:15 #\Preparing to sleep until Mon Sep 17 06:00:15 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST17-Sep-18 #\Continuing at 06:00CEST17-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:18:15CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST17-Sep-18" +235164 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1507h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*f  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*/% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1509h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*A  +11 h  F*>% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*i  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +21 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*Q  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*(  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*+'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1509h.pcr F*G% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*O  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +87 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *9'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*K% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*h'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1512h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*<  +11 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *q'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*$ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*- n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*V  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*k'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*0  +108 h  ."MFBPCR"delay 7 seconds +700 F*P% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*c'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*d  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*%% h % \\ ."MFBPCR"\AS.seek 0.326ml F*A'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1512h.pcr F*\ % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*Q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 0.47ml F*,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*!% h %m ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1514h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*v  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*@ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*p  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*.'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1514h.pcr F*/% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*T  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*Y% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*V  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*c % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*z% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1516h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*F  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*\\% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*O  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +21 h  ."MFBPCR"delay 7 seconds +700 F*m% h %< ."MFBPCR"\AS.seek 0.594ml F*U'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*K n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*u  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1516h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*W'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +65 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*{% h % ."MFBPCR"\AS.seek 0.81ml F*<'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*#'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1519h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*0% h %V ."MFBPCR"\AS.seek 0.5ml F*P'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*H n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*c  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*% I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*j % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*>' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +21 h n ."MFBPCR"delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*(  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1519h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Z  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +424 h ' ."MFBPCR"\ATV.dial :ASV F*= n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F*g% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*\\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *!' +424 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*e% h % ."MFBPCR"\AS.seek 0.71ml F*K' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*U% h % H ."MFBPCR"\AS.seek 0.735ml F*u' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*D% h % Ű ."MFBPCR"\AS.seek 0.72ml F*n' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*h n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*w% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*?% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*S' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %  ."MFBPCR"\AS.seek 0.604ml F*v'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*W' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*: % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*^' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h % q ."MFBPCR"\AS.seek 0.605ml F*B'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*1% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*;' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*{  +21 h  ."MFBPCR"\AS.seek 0ml F*f'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*<  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *T' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*~ n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *#'R +202 h 'R ."MFBPCR"\SPE.coast F*"  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*2 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*$  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*- I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*V  +43 h  F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*0  +65 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *P'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*H n +65 h n F*k% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *R' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*_  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*j% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *\ 'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*d n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *z' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*> I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*i  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *A': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*8 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *]'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*s n +150 h n ."MFBPCR"\AS.seek 0ml F*J'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*Q  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*' % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*F'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F* 'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*= I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *g'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\\'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *G' +9987 h ' ."MFBPCR"\ATV.dial AIV F*b I +43 h I ."MFBPCR"\AS.seek 0.02ml F*!' +9880 h ' ."MFBPCR"\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.seek 0ml F*e'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*C% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*U  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*w'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*# % +108 h % ."MFBPCR"\AS.seek 0ml F*2'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537156815.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )$ h  C' ' h  P% %Y h  F* *- h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *&% h %V .t\AS.configure ASconfig S)V% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X' +21 h '\  ."closeHand"Hand.close M&z/ .t\Elbow.jog 1500 M&i* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b ''4 S)#.0X 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:19:12CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +150 h 1 M&=& h &_ M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &`(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &\\'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +265 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'!& h &W .tHand.open M&e1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'a& h &W C'9% h %4 .t\CSV.dial CSR C'u  +151 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'w +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Purging external Intake C'?& h &W C'% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X 'N  h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'r'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'& h &W C'y% h %4 .t\CSV.dial :flush C'v  +76 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'L% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C': +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'}& h &W C'% h %4 .t\CSV.dial :flush C'.  +38 h  .t\CS.seek 3ml C'p'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'^% h %0 .t\CS.seek 5ml C'1'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'{ +75 h .t\CS.seek 0ml C';'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&T/ +150 h / .t\CC.seek :open C'"( +416 h ( M&~& h &ӷ M&#% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +265 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &)'η +91 h 'η .tHand.open M&I1 +150 h 1 M&4% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +90 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:19:16CEST30-Aug-18\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:19:12CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*2% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&$% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(Y. h ., A(-& h &  .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&&& h &- M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &6( +7 F*q% h %&K ."readyMFB"\ATV.dial :waste2 F*0 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *5' +159 h ( M&t& h & A(H& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &k' +90 h ' A(P& h &  .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(R& h &  M&c& h & .t\Elbow.seek 2 M&_( +6 h ( M&%& h & A(j& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &d/ +150 h / ."downElevator"\SE.seek :down M&& A(|'X h & M&\ % h %; .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.seek CC M&s(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &(' +91 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)'+ +6 h + S)J% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 S)g% h %5J .t\SSV.dial :spit S)+ +132 h ' ."readyMFB"\AFV.select :carrierSolution F* +6 ."readyMFB"delay 2 seconds +12 h .t\SS.seek 0ml S)' +188 ."readyMFB"\AS.seek 1ml F*' +28 h ' .t\SSV.dial CBV S)  +151 h  .t\CTV.dial :intake C'  +75 h  #tWaiting for external sampler to finish priming... .delayUntil "06:08:51.30" +5031 h ' ."readyMFB"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."readyMFB"delay 1 second +100 .\ATV.dial :waste F* n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *\\'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*b  h  ."readyMFB"\ASV.dial :waste1 F*G  h +18246 ."connect"\SSV.dial CBV .t\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'`% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X9 h 9d +100 C'g9 h 9d +100 C'+9 h 91d +100 C'9 h 9J d +100 C'9 h 9bd +100 C'9 h 9zd +100 C'59 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'b9 h 9$d +100 C'G9 h 9(d +100 C'`9 h 9 ,d +100 C'9 h 9$0d +100 C'Z9 h 9<4d +100 C'!9 h 9S8d +100 C'e9 h 9kd +100 C'y9 h 9Td +100 C' 9 h 9kd +100 C'v9 h 9d +100 C'r9 h 9d +100 C'L9 h 9d +100 C' 9 h 9d +100 C':9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C' 9 h 9 d +100 C'9 h 96d +100 C'@9 h 9Ld +100 C'}9 h 9bd +100 C'9 h 9xd +100 C'W9 h 9d +100 C'9 h 9d +100 C'o9 h 9d +100 C'^9 h 9d +100 C'.9 h 9d +100 C'p9 h 9d +100 C'B9 h 9d +100 C'{9 h 9(d +100 C'f9 h 9>d +100 C'19 h 9Td +100 C'/9 h 9id +100 C'9 h 9d +100 C'<9 h 9d +100 C'9 h 9d +100 C'T9 h 9d +100 C'K9 h 9d +100 C'C9 h 9d +100 C'#9 h 9  d +100 C'x9 h 9 d +100 C'O9 h 9 *d +100 C'9 h 9 ?d +100 C'[9 h 9 Td +100 C'I9 h 9 j d +100 C'~9 h 9 $d +100 C'79 h 9 (d +100 C'9 h 9 ,d +100 C'29 h 9 0d +100 C'$9 h 9 4d +100 C'9 h 9 8d +100 C'Y9 h 9 'p +1372 h 'p .t\CSV.dial CTV C'g  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'+ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'5 +113 h .t\CBV.dial CSV C'  +19 h C'% h %4 .t\CS.seek 5ml C'b'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'G  +38 h C'`% h %p .t\CS.seek 7ml C''i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'Z @ +38 h @ .t\CS.seek 10ml C'!' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'e ` +132 h ` #tLysing Complete C'U& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&a% h %\  A(E% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (m( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&n& h &ӷ M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &u( +142 h ( M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &?' +89 h ' A(& h & .t\SE.seek 4.71 Pucks below Top Plate A(3' +215 h ' M&D& h & A(N& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &X'Y +25 h 'Y .t\Elbow.hold M&S, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ .t\SE.seek 5.04 Pucks below Top Plate A(;' +27 h ' M&y% h %Y A( & h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&v'υ +21 h 'υ M&r& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(:' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'( +416 h ( A(o& h & A(^& h & M&.& h &ӷ M&p% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &{( +142 h ( M&f& h & A(1& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &/'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &<1 +150 h 1 M&% h %Y A(T& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&K'υ +21 h 'υ M&C& h & A(#& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &x' +22 h ' A(O% h % ."downElevator"\SE.seek :down A('X +100 ([% h % M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'7 @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'2 ` +19 h ` .t\AIV.dial :bypass C'$ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&Y1 +2 h 1 M&-& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&4(- +166 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&0'η +91 h 'η .tHand.close M&/ +150 h / M&H& h &- M&)% h %η .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'l(W +416 h (W C'R& h &W .tHand.open M&1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%j +150 h P%\ % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%k  +38 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%i' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'  h +75 .t\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%z  h .t\PS.seek 0ml P%|' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :mixloop P%J +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%8' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%'' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&Q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +6 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'>( +416 h ( M&5& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +90 h 'η .tHand.open M&Z1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'e& h & M&U% h %\  A(a% h %X A(E. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (h( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&n& h &- M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &w( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &s' +89 h ' A(A& h &  .t\SE.seek 13.71 Pucks below Top Plate A(D'n +125 h 'n A(N& h &  M&& h & .t\Elbow.seek 2 M&y( +7 h ( M& & h & A(r& h &  .t\Forearm.seek 2 + 300 counts M&L'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(?'X h & M&u% h %; .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.seek CC M&X(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +109 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +90 h ' .t\CC.seek :closed C';(W +416 h (W .tHand.open M& 1 +150 h 1 M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C',  +19 h  .t\CS.seek 5ml C'o'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'^' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'.'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%p  +38 h  .t\PS.seek 2.5ml P%f' +2610 h ' .t\PSV.dial :mixloop P%1 +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%<  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'T @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'C  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'# @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'O  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'B ` +19 h ` .t\CSV.dial :air C'{ @ +38 h @ .t\CS.seek 10ml C'[' +2647 h ' .t\CSV.dial CTV C'I  +38 h  .t\CS.seek 0ml C'7'4 +2647 h '4 .t\CBV.dial :waste1 C'2 @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'$  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&Y% h %\  A(-% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (/( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (K& h & M&:& h &ӷ M&x% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &V( +142 h ( M&q& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(!& h & .t\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' M&& h & A(H& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &)'Y +25 h 'Y .t\Elbow.hold M&~, +6 h , .t\Elbow.reconfigure TightElbow A(0- M&$&.,(\ #b h -+ .t\SE.seek 6.04 Pucks below Top Plate A(M' +27 h ' M&c% h %Y A(4& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&P'υ +21 h 'υ M&& h & A(R& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(&' +36 h ' M&j& h & M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +89 h '\  .t\Elbow.seek CC M&k(ӷ +143 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(_& h & A(z& h & M&i& h &ӷ M&% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &J( +142 h ( M&"& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M& , +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &81 +150 h 1 M&Q% h %Y A(g& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &+'υ +20 h 'υ M&& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +23 h ' A(l% h % ."downElevator"\SE.seek :down A(5'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%`  + A(6 +111 h  P%(% h % .t\PS.seek 0.499ml P%Z' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%= +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%e  +38 h .t\CBV.dial AIV C'U  +38 h .t\PRV.dial between CBV and air P%a  +19 h  .t\PRV.dial :air P%E  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %n' +251 h ' .t\PRV.dial :waste2 P%9 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %b' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&A& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +264 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'L(W +416 h (W C'D& h &W .tHand.open M&y1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  C'>& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%S  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%,% h % .t\PS.seek 1.499ml P%v' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%h' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%o' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%^ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%p  +38 h  .tdelay 2 seconds +200 P%'% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %1'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%<' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%f  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%T'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%C  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%#  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %O' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%B +75 h .t\PTV.dial :PRV,avoiding: :puck P%{ @ +75 h @ .t\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%[' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%2 +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%$' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%- +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%K% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %:' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'x & +50 h & .t\AWV.select :waste C' +6 P%q% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %m'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'! { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %X' +1312 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%H' +2610 h ' .t\PSV.dial :PML P%W +37 h .t\PS.seek 0ml P%0' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'c& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'4& h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F* @@T .t\AIV.dial :bypass F*R  .t\PSV.dial PSR P%j +21 h  ."MFBPCR"delay 20 seconds +91 h P%\ % h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*V I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *)'6 +66 .t\PSV.dial :waste1 P%6  +38 h  .t\PS.seek 0ml P%*' +1597 h ' .t\PSV.dial PSR P%P +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%M' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%~' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%% +21 h  F*;% h %6 ."MFBPCR"\AS.seek 0.329ml F*_'ͯ +92 h P%z% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%i' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%]' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*k  +21 h  F*8% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *Q' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%g +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*t I +43 h I F*l% h % ."MFBPCR"\AS.seek 0.469ml F*|' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%+' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%`' +739 h ' ."MFBPCR"delay 5 seconds +500 F*(9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*d n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*5% h % ."MFBPCR"\AS.seek 0.041ml F*/'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%=' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%e' +2609 h ' .t\PSV.dial PSR P%U +113 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%E' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%Z' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%9 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%n' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %A'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b * 'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %G' +5106 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %s' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*@  +43 h  F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *u'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*D% h %Q ."MFBPCR"\AS.seek 0.01ml F*y' +171 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %>' +619 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek :empty F*}'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*S  +86 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *,' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*b  +43 h F*v% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'h  +37 h  C'^% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C''' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'o  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'1'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*f  +22 h ."MFBPCR"delay 3 seconds +300 F*<% h %_ ."MFBPCR"\AS.seek 0.434ml F*C'ݏ +110 h '4 .t\CSV.dial CSR C'#  +37 h  C'T% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'B'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*{  +22 h F*O% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*I'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*. n +107 h n F*2% h %& ."MFBPCR"\AS.seek 0.092ml F*['&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'-'4 +2647 h '4 C' & h &W #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&:' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'm( +416 h ( M&& h &ӷ M&X% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&j1 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'V& h & M&% h %\  A(6% h %X A(\\. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (P& h &  M&*& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &M( +166 h ( M&& h & A(;& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(z& h &  ."WCR"\SE.seek 12.71 Pucks below Top Plate A()' +137 h ' A(~& h &  M&& h & ."WCR"\Elbow.seek 2 M&_( +6 h ( M&& h & A(k& h &  ."WCR"\Forearm.seek 2 + 300 counts M&8'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &i/ +119 h '&0 ."MFBPCR"delay 3 seconds +31 h / ."downElevator"\SE.seek :down M&"& h & A(g'X M&t% h %; ."WCR"\Forearm.seek :retract M&l'\ +91 h '\  ."WCR"\Elbow.seek CC M&](ӷ +46 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&Q& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +35 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +13 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&&1 +130 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +20 h 1 M&(% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *5' +89 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)|+ +6 h + S)% h % C'`& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:20:42CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST19-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1709h25ml.pcr F**% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*z  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*g  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*x% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*E% h %m ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*y  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*b  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*u' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*v  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*h'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*T  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*A% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1711h25ml.pcr F*'% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*I  +22 h ."MFBPCR"\ATV.dial :air3 F*` % +87 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*$% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*X  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +31 h  ."MFBPCR"\ATV.dial :air3 F*3 % +87 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*H  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*x  +22 h ."MFBPCR"delay 2 seconds +200 F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *q'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*] n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*m' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*E  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*5'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1713h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*r  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*_% h %n ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +64 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*# % +86 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *A' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*o% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*<  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +11 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\\  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*3  +21 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *['Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*Y n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F** I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*P n +21 h n ."MFBPCR"delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1716h25ml.pcr F*"% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*+'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*e  +64 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*5 % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*v  +10 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*#  +22 h ."MFBPCR"delay 2 seconds +200 F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*{ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*=  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*C' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*I  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +21 h  ."MFBPCR"delay 7 seconds +700 F*.% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*$ n +21 h n ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1718h25ml.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*:  +22 h ."MFBPCR"\ATV.dial :air3 F*Z % +87 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +21 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +99 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*i  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*%% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +21 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ARV.dial :carrier F*+  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *|'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*U I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*/% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*9 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*G n +21 h n ."MFBPCR"delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*D' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*_  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*u @@T ."MFBPCR"\ASV.dial :SPEdelivery F*h  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *1' +424 h ' ."MFBPCR"\ATV.dial :ASV F*# n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*T  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*{'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*o n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *='V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*''(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*A % +43 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *`' +424 h ' ."MFBPCR"\ATV.dial :air3 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*$' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h % H ."MFBPCR"\AS.seek 0.735ml F*-' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*f% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*2 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*c% h % zG ."MFBPCR"\AS.seek 0.365ml F*K' +7162 h ' ."MFBPCR"\ATV.dial :waste F*n n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*W% h % ."MFBPCR"\AS.seek 0.579ml F*?' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %  ."MFBPCR"\AS.seek 0.604ml F*\\'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*V% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*['V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*\ % h %V ."MFBPCR"\AS.seek 0.01ml F*~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*z% h % ."MFBPCR"\AS.seek 0.58ml F*P' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h % q ."MFBPCR"\AS.seek 0.605ml F*i'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*%  +22 h  ."MFBPCR"\AS.seek 0ml F*M'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *"'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *Q' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*8 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *H'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*J n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +202 h 'R ."MFBPCR"\SPE.coast F*x  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *g' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*E  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *+': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*d  +65 h  F*e% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*a% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *@' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b */': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*|  +65 h  F*9% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*r% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*G n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *y'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*s'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*b  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*D% h % ."MFBPCR"\AS.seek 0.96ml F*v'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %0 ."MFBPCR"\AS.seek 0.985ml F*S' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*7'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*# I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*=  +42 h  F*A% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *I' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *'' +9987 h ' ."MFBPCR"\ATV.dial AIV F*C I +43 h I ."MFBPCR"\AS.seek 0.02ml F*`' +9880 h ' ."MFBPCR"\ATV.dial :waste F*. n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*<% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +21 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*W  +65 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*~'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +21 h F*k% h %m ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' @CEST1537221616.45 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*x n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *H'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*l % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537329630.89 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' '! h  P% %( h  F* *] h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *g  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)E% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'm& S)#.0X 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:21:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&S1 +150 h 1 M&#& h &_ M&7% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &T(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &o'η +91 h 'η .tHand.close M&{/ +150 h / M&=& h &- M&A% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +264 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +91 h ' .t\CC.seek :closed C'<(W +416 h (W C'`& h &W .tHand.open M& 1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'B% h %4 .t\CSV.dial CSR C'-  +150 h  C'2% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'f'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'c +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +2009 h '4 #t\Purging external Intake C'j& h &W C'K% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C': 'N  h +75 .t\CTV.dial :intake C'3  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'6'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'?  +75 h #t\Flushing upper manifold C'Y& h &W C'V% h %4 .t\CSV.dial :flush C'\\  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'[% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'4 +37 h .t\CS.seek 0ml C'z'4 +507 h '4 C'& h &W C';% h %4 .t\CSV.dial :flush C'k  +37 h  .t\CS.seek 3ml C'~'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'P% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'% +76 h .t\CS.seek 0ml C'0'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&M& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&8' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'x( +416 h ( M&J& h &ӷ M&l% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &R(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &>'η +91 h 'η .tHand.open M&}1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:21:44CEST30-Aug-18\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:21:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'u& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(!% h %X A((. h ., A(]& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (g& h &  M&& h &- M&E% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &m( +6 F*q% h %&K ."readyMFB"\ATV.dial :waste2 F*d % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *e' +160 h ( M&+& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &a' +89 h ' A(& h &  .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ A(@& h &  M&5& h & .t\Elbow.seek 2 M& ( +6 h ( M&U& h & A(|& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&r& A(9'X h & M&% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +54 h 'X ."downElevator"\SE.coast A(5 +88 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'p(W +416 h (W .tHand.open M&h1 +150 h 1 M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)s% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S) +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'V9 h 9Td +100 C'39 h 9kd +100 C'\\9 h 9d +100 C'69 h 9d +100 C'[9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'*9 h 9d +100 C'\ 9 h 9 d +100 C'X9 h 9 d +100 C'Y9 h 96d +100 C'9 h 9Ld +100 C'9 h 9bd +100 C';9 h 9xd +100 C'9 h 9d +100 C'z9 h 9d +100 C'9 h 9d +100 C'P9 h 9d +100 C'k9 h 9d +100 C'~9 h 9d +100 C'i9 h 9d +100 C'%9 h 9(d +100 C'M9 h 9>d +100 C'9 h 9Td +100 C'"9 h 9id +100 C'49 h 9d +100 C'9 h 9d +100 C't9 h 9d +100 C'Q9 h 9d +100 C'$9 h 9d +100 C'<9 h 9d +100 C'l9 h 9  d +100 C'89 h 9 d +100 C'I9 h 9 *d +100 C'9 h 9 ?d +100 C'F9 h 9 Td +100 C'}9 h 9 j d +100 C'J9 h 9 $d +100 C')9 h 9 (d +100 C'H9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'!9 h 9 8d +100 C'(9 h 9 9 h 9\ `d +100 C'9 h 9\ dd +100 C'+9 h 9\ hd +100 C'R9 h 9\ ld +100 C'59 h 9 pd +100 C'U9 h 9 td +100 C'w9 h 9 /xd +100 C'@9 h 9 C|d +100 C'9 h 9 Wd +100 C'9 h 9 kd +100 C'y9 h 9 d +100 C'|9 h 9 d +100 C'r9 h 9 d +100 C'm9 h 9 d +100 C'a9 h 9 d +100 C'9 h 9 d +100 C'^9 h 9 d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'9 h 9 1d +100 C' 9 h 9 Dd +100 C'9 h 9 Xd +100 C'99 h 9 kd +100 C'h9 h 9 ~d +100 C'x9 h 9 d +100 C's9 h 9 d +100 C'9 h 9 d .t\CH.hold 5:00,85C C'/   P h  +30000  C'b9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C' '4 +423 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +112 h @ .t\CSV.dial :air C'G @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'1 @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'S  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'= @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'A'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'e +113 h .t\CBV.dial CSV C'D  +19 h C'{% h %4 .t\CS.seek 5ml C'C'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C''  +38 h C'T% h %p .t\CS.seek 7ml C'_'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +38 h @ .t\CS.seek 10ml C'`' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C' ` +131 h ` #tLysing Complete C',& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M& % h %\  A(O% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (.( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (&& h & M&& h &ӷ M&B% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &-( +142 h ( M&c& h & A(n& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &j' +90 h ' A(K& h & .t\SE.seek 7.71 Pucks below Top Plate A(Z' +184 h ' M&f& h & A(N& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &:'Y +26 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow A(W- M&$&.,(\ #b h -+ .t\SE.seek 8.04 Pucks below Top Plate A(0'w +27 h 'w M&V% h %Y A(3& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &\\'υ +20 h 'υ M&6& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A('f +37 h 'f M&*& h & M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&Y(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'z( +416 h ( A(& h & A(P& h & M&k& h &ӷ M&~% h % .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &%( +143 h ( M&M& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &"'Y +92 h 'Y .t\Elbow.hold M&4, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&t% h %Y A(Q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &$'υ +21 h 'υ M&<& h & A(l& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &8' +22 h ' A(I% h %f ."downElevator"\SE.seek :down A('X +100 (F% h % M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +72 h 'X ."downElevator"\SE.coast A(5 +18 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C') @ +18 h @ .t\CS.seek 10ml C'H' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'!& h & .tHand.open M&(1 +2 h 1 M&]& h & M&g% h %\  .t\Elbow.seek FlushPuck::Garage M&L(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&d'η +91 h 'η .tHand.close M&u/ +150 h / M&& h &- M&>% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &U' +91 h ' .t\CC.seek :closed C'w(W +416 h (W C'@& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%| +150 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%a  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%^ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%9' +2609 h ' .t\PSV.dial :air P%h  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%s +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%/  +38 h  .t\PS.seek 2.5ml P%b' +2609 h ' .t\PSV.dial :mixloop P%# +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'( +416 h ( M&e& h &ӷ M&D% h % .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +264 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&,% h %\  A( % h %X A(O. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (2( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (&& h &  M&& h &- M&B% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &c( +166 h ( M&n& h & A(K& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(f& h &  .t\SE.seek 10.71 Pucks below Top Plate A(N' +155 h ' A(& h &  M&W& h & .t\Elbow.seek 2 M&V( +7 h ( M&3& h & A(6& h &  .t\Forearm.seek 2 + 300 counts M&['; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down M&\ & h & A(j'X M&-% h %; .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.seek CC M&:(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +78 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'0(W +416 h (W .tHand.open M&1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'X  +151 h  .t\CSV.dial CTV C'p  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'P' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'k'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%M' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%;  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Y +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%t' +2609 h ' .t\CBV.dial :waste1 C'Q @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'<  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'l @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'I  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'i ` +19 h ` .t\CSV.dial :air C'% @ +38 h @ .t\CS.seek 10ml C'F' +2647 h ' .t\CSV.dial CTV C'}  +37 h  .t\CS.seek 0ml C')'4 +2648 h '4 .t\CBV.dial :waste1 C' @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&(% h %\  A(]% h %X A(g. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ("( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ($& h & M&& h &ӷ M&8% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &E( +143 h ( M&q& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &.' +89 h ' A(`& h & .t\SE.seek 8.71 Pucks below Top Plate A(4' +175 h ' M&u& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &>'Y +26 h 'Y .t\Elbow.hold M&J, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(d- h -+ .t\SE.seek 9.04 Pucks below Top Plate A(R'f +27 h 'f M&5% h %Y A(L& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'υ +20 h 'υ M&z& h & A(@& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' .t\SE.seek 10.04 Pucks below Top Plate A('U +36 h 'U M&|& h & M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  .t\Elbow.seek CC M&a(ӷ +143 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h & A(& h & M&& h &ӷ M&h% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &s( +142 h ( M&x& h & A(/& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&#, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &b1 +150 h 1 M&G% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &1'υ +20 h 'υ M&=& h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' A(w% h %U ."downElevator"\SE.seek :down A(e'X +100 (D% h % M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +62 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast .\PSV.dial :air A(6 P%T  +113 h  P% % h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%7 +38 h .t\PRV.dial :air P%{  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C',  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%O  +19 h  P%&% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%B @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&K1 +2 h 1 M&f& h & M&A% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&W& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&6'η +91 h 'η .tHand.close M&_/ +150 h / M&*& h &- M&\ % h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'N& h &W .tHand.open M&V1 +150 h 1 M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%?  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%X  +75 h  .tdelay 2 seconds +200 P%p% h % .t\PS.seek 1.499ml P%\\' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%2' +1094 h ' .t\PSV.dial :air P%H  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%P +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%~  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%;  +75 h .tdelay 2 seconds +200 P%Y% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%M  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Q'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%<  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%l  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %I' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%i +75 h .t\PTV.dial :PRV,avoiding: :puck P%% @ +76 h @ .t\PS.seek 0ml P%k' +2609 h ' .t\PSV.dial :air P%}  +76 h  .t\PS.seek 2.5ml P%F' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +76 h  .t\PS.seek 2.5ml P%(' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%] +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%g  +75 h P%$% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'8 & +50 h & .t\AWV.select :waste C' +7 P%q% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'` { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%u  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%d +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '5& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'L& F*z @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*@  .t\AIV.dial :bypass .\PSV.dial PSR P%| +22 h  ."MFBPCR"delay 20 seconds +91 h P%r% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%.' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*E I +128 h I F*4% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *>'6 +66 .t\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%R' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%U +21 h  F*0% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*h % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%/  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*a  +22 h  F*b% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *G' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*w% h % ."MFBPCR"\AS.seek 0.469ml F*9' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%s +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial PSR P%S +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%T' +740 h ' ."MFBPCR"delay 5 seconds +500 F* 9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*y n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*e% h % ."MFBPCR"\AS.seek 0.041ml F*"'% +336 h ' .t\PSV.dial PSR P%t +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%{ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%, +113 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%B +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%K +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %f'k +2678 h 'k .t\PSV.dial :waste1 P%A  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %W' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *#'V +850 h ' .t\PSV.dial :air P%_  +113 h  .t\PS.seek 2.5ml P%*' +2609 h ' .t\PSV.dial :mixloop P%\ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %'' +5107 h ' .t\PSV.dial :air P%6  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *-'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*N% h %Q ."MFBPCR"\AS.seek 0.01ml F*V' +171 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*?  +85 h  F*X% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *p' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*\\% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *H'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'2  +38 h  C'P% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C';  '  +37 h  +113  .t\CBV.dial :waste1 C'Y @ +38 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*M  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*<'ݏ +110 h '4 .t\CSV.dial CSR C'l  +38 h  C'Q% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'i'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*%  +21 h F*I% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*}'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*k n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*F'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C']'4 +2648 h '4 C'g& h &W #tGaraging FlushPuck in Collection Clamp M&$& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +6 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'( +416 h ( M&u& h &ӷ M&:% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &@'η +91 h 'η .tHand.open M&|1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'E& h & M&4% h %\  A(m% h %X A(o. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (.( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&^& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &R( +165 h ( M&& h & A(0& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +90 h ' A(& h &  ."WCR"\SE.seek 9.71 Pucks below Top Plate A(>' +166 h ' A(J& h &  M&h& h & ."WCR"\Elbow.seek 2 M& ( +6 h ( M&/& h & A(a& h &  ."WCR"\Forearm.seek 2 + 300 counts M&b'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +88 h '&0 ."MFBPCR"delay 3 seconds +62 h / ."downElevator"\SE.seek :down A('X M&x& h & M&+% h %; ."WCR"\Forearm.seek :retract M&w'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&G& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +5 ."MFBPCR"\ATV.dial :waste2 F*S % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +43 h ' ."WCR"\CC.seek :closed C'D(W +416 h (W ."WCR"Hand.open M&1 +101 h 'R ."MFBPCR"\ATV.dial :air2 F*1  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b * ' +28 h 1 M&y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)9+ +6 h + S)t% h % C'T& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'{  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*b  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*w' +625 h ' ."MFBPCR"\ATV.dial :air3 F*F % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*1' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*y% h % ."MFBPCR"\AS.seek 0.23ml F*t' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +21 h F*.% h %X ."MFBPCR"\AS.seek 0.24ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *O'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*7 @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*" I +86 h I ."MFBPCR"\AS.seek 0ml F*K'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*A  +43 h  #"MFBPCR"\Swish #1 of 2 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1031 h '\  F*n% h %\  ."MFBPCR"\AS.seek 0.5ml F*_'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*W% h %(A ."MFBPCR"\AS.seek 0.6ml F*\ '\  +1031 h '\  F*f% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*# % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b **'V +3472 h 'V ."MFBPCR"\SPE.coast F*6  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1909h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*N  +64 h  ."MFBPCR"\ARV.dial :carrier F*j  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*p  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*P% h %Fj ."MFBPCR"\AS.seek 0.524ml F*2' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*~  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*)' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Y % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*l% h %< ."MFBPCR"\AS.seek 0.594ml F*Q'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*<  +43 h  ."MFBPCR"\ASV.dial :waste1 F*}  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*!'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*$ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254631 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'8  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'g @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '`' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'c @ +75 h @ +50 S)q% h % ."WCR"\CBV.dial between CSV and waste2b C'[  +94 h  C'u% h % ."WCR"\CSV.dial between air and CTV C'Z ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'5  ':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '('4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'|  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'd  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'r  +38 h  C'4% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'o' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'E @ +76 h @ +50 S)m% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'@% h % ."WCR"\CSV.dial between air and CTV C'^ ` +56 h ` ."WCR"\CS.seek 7.288ml C'0' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'J'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'h  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'a  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'/% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'R @ +75 h @ +50 S)U% h % ."WCR"\CBV.dial between CSV and waste2b C'+  +94 h  C's% h % ."WCR"\CSV.dial between air and CTV C'S ` +56 h ` ."WCR"\CS.seek 7.288ml C'G' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'b  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'>  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'F' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'w& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(y% h %X A(1. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (T( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ({& h & M&t& h &ӷ M&=% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &.( +143 h ( M&& h & A( & h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(9& h & ."WCR"\SE.seek 9.71 Pucks below Top Plate A(&' +166 h ' M& & h & A(O& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &"'Y +25 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(K- M&$&.,(\ #b h -+ ."WCR"\SE.seek 10.04 Pucks below Top Plate A(A'U +27 h 'U M&B% h %Y A( & h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&n'υ +21 h 'υ M&3& h & A(W& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' ."WCR"\SE.seek 11.04 Pucks below Top Plate A(f'D +37 h 'D M&\ & h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +89 h '\  ."WCR"\Elbow.seek CC M&#(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ ."WCR"\Forearm.seek CC M&N' +91 h ' ."WCR"Hand.close M&j/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(*& h & A( & h & M&V& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&?'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&C& h & A(\\& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y ."WCR"\Elbow.hold M&p, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &P1 +150 h 1 M&-% h %Y A(~& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&;'υ +21 h 'υ M&2& h & A(Y& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A()% h %D ."downElevator"\SE.seek :down A('X +100 (l% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +52 h 'X ."downElevator"\SE.coast A(5 +38 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&Q1 +2 h 1 M&I& h & M&%% h %\  .t\Elbow.seek FlushPuck::Garage M&H(- +165 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&k& h &- M&i% h %η .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +265 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'q(W +416 h (W C'[& h &W .tHand.open M&u1 +150 h 1 M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'5% h % .t\CSV.dial CSR C'  +37 h  C'|% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'('p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'd% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'r'[ +463 h '[ .t\CSV.dial :waste C'4 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'o& h &W C'E% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'^% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C'0 @ +76 h @ .t\CS.seek 0ml C'h'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'J& h &W C'a% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  +37 h  .t\CBV.dial :waste2b C'+  +76 h  .t\CS.seek 0ml C's'4 +766 h '4 #t\Flushing upper manifold C'S& h &W C'x% h %4 .t\CSV.dial :flush C'b  +75 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'>% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'w  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'y'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'1'0 +507 h '0 .t\CSV.dial :waste C'{ +37 h .t\CS.seek 0ml C't'4 +507 h '4 C'=& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'9'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C' % h %0 .t\CS.seek 5ml C'O'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'e'0 +507 h '0 .t\CSV.dial :waste C'B +75 h .t\CS.seek 0ml C' '4 +507 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'W% h %4 .t\CSV.dial CSR C'\  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C','L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'.' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& +113 h .t\CTV.dial :intake C'"  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'6  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'A  +113 h  C'_% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +710 h ' ."connect"\CSV.dial CTV C''  .t\CTV.dial CSV C'D  +37 h  +113  .t\CBV.dial :waste1 C'* @ +38 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'V  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'C'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  +37 h  .t\CBV.dial :waste2b C'#  +76 h  .t\CS.seek 0ml C'P'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'-  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '2'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C')  '? +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'l'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'Q'4 +506 h '4 #t\Flushing upper manifold C'I& h &W C'%% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'<% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'T'0 +506 h '0 .t\CSV.dial :waste C'p +38 h .t\CS.seek 0ml C''4 +506 h '4 C'k& h &W C'i% h %4 .t\CSV.dial :flush C'M  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'g @ +37 h @ C']% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'[ +75 h .t\CS.seek 0ml C'u'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'Z& h &W C':% h %4 .t\CSV.dial :flush C'5  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'c% h %p .t\CS.seek 7ml C'F'i +415 h 'i ."connect"\CSV.dial CTV C'd  .t\CTV.dial CSV C'(  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +38 h @ .t\CS.seek 0ml C'r'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'o& h &W C'E% h %4 .t\CSV.dial :flush C'm  +75 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'^% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'0'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'J% h %4 .t\CSV.dial CSR C'a  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'h'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'/  +38 h +75 .t\CTV.dial :intake C'U  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'+' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'R  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'S'4 +607 h '4 .t\CSV.dial CSR C'x  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C's'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1373 h ' .t\CSV.dial CBV C'> +113 h .t\CS.seek 0ml C' '4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&w& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&y' +91 h ' .tHand.close M&{/ +150 h / .t\CC.seek :open C'1( +416 h ( M&=& h &ӷ M&% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &9'η +90 h 'η .tHand.open M&O1 +150 h 1 M&K% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&31 +2 h 1 M&W& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &\ (- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&,'η +91 h 'η .tHand.close M&&/ +150 h / M&"& h &- M&.% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' .t\CC.seek :closed C'(W +416 h (W C''& h &W .tHand.open M&D1 +150 h 1 M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'V& h &W C'% h %4 .t\CSV.dial CSR C'  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '#% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '\\'[ +463 h '[ .t\CSV.dial :waste C'- +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +2010 h '4 #t\Purging external Intake C'P& h &W C'2% h %4 .t\CSV.dial :flush C')  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'Y  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'l'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'I% h %4 .t\CSV.dial :flush C'%  +75 h  .t\CS.seek 5ml C'X'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +38 h @ C'f% h %p .t\CS.seek 7ml C'<'i +416 h 'i ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'}'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'p'4 +506 h '4 C'T& h &W C'k% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 3ml C'M'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'g% h %0 .t\CS.seek 5ml C']'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'$ +75 h .t\CS.seek 0ml C'['4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&u' +90 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'c( +416 h ( M&& h &ӷ M&d% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +265 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &E'η +91 h 'η .tHand.open M&m1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Completed [mbaPhase] begun 06:00:46 #\Preparing to sleep until Fri Sep 21 06:00:46 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:00CEST21-Sep-18 #\Continuing at 06:00CEST21-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:23:10CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:00CEST21-Sep-18" +235183 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1909h25ml.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*x% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*{ % +87 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*=% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1911h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*,  +42 h  ."MFBPCR"\ARV.dial :carrier F*A  +11 h  F*_% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F**  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*C  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*#  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*-' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\\  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*2'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +108 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +21 h n ."MFBPCR"delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep1911h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*}  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *i'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*|% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*L'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1913h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*b  +10 h  F*S% h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *q'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*= I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*O  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +107 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*.  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*A'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*B % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep1913h25ml.pcr F*\ % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*6  +22 h ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*#'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*l % +87 h % F*Q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*<'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*T% h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1916h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*j'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *F'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*^ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*z' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*R  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*S % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep1916h25ml.pcr F*x% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*G  +22 h ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*'% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*1  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *-'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep1918h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*)  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*? n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*l  +22 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*% n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*;% h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*7  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*}  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*]  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*$' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*H  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*|'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep1918h25ml.pcr F*5% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*m% h %Z ."MFBPCR"\AS.seek 0.81ml F*u'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*F'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*w'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1920h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +10 h  F*K% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*W n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *9'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*, I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*B % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*_' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +21 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*#  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*P% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep1920h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*- @@T ."MFBPCR"\ASV.dial :SPEdelivery F*2  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Y  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*l n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*Q  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*%'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*; n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *7'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *T' +423 h ' ."MFBPCR"\ATV.dial :air3 F*<% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*X% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h % H ."MFBPCR"\AS.seek 0.735ml F*]' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*M% h % Ű ."MFBPCR"\AS.seek 0.72ml F*H' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*:% h % zG ."MFBPCR"\AS.seek 0.365ml F*$' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*5 % +43 h % F*|% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*d% h % ."MFBPCR"\AS.seek 0.579ml F*j' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %  ."MFBPCR"\AS.seek 0.604ml F*o'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*E% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*!  +21 h  ."MFBPCR"\AS.seek 0ml F*F'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*z % +108 h % F*r% h %V ."MFBPCR"\AS.seek 0.01ml F*J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*/% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*+ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AS.seek 0ml F*R'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *x'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*b  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *G' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*> n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*s n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +202 h 'R ."MFBPCR"\SPE.coast F*8  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*{ n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*=  +43 h  F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*O  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *y': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*K  +65 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *e'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*W n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*& I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *"': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*9  +65 h  F*B% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *\ 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*t n +64 h n F*6% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *'' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*_ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F**  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *A': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*D n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *V'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*n % +42 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*N% h % ."MFBPCR"\AS.seek 0.96ml F*\\'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %0 ."MFBPCR"\AS.seek 0.985ml F*)' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*?'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*l I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *;'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *I'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*7  +43 h  F*f% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *}' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*< I +43 h I ."MFBPCR"\AS.seek 0.02ml F*T' +9880 h ' ."MFBPCR"\ATV.dial :waste F*k n +129 h n ."MFBPCR"\AS.seek 0ml F*X'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *M'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*|% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Z'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' @CEST1537394431.76 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*w % +108 h % ."MFBPCR"\AS.seek 0ml F*{'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537502446.33 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )= h  C' '` h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)O% h % .t\SS.configure SSconfig C'& S)#.0X 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:24:07CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #5) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&)1 +150 h 1 M&l& h &_ M&?% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &Q(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &;'η +91 h 'η .tHand.close M&%/ +150 h / M&7& h &- M&f% h %η .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'T& h &W .tHand.open M&X1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'i% h %4 .t\CSV.dial CSR C']  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'M'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'H% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C': +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '5'4 +2010 h '4 #t\Purging external Intake C'|& h &W C'$% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'd' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  h +75 .t\CTV.dial :intake C'Z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'm'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'j  +75 h #t\Flushing upper manifold C'(& h &W C'E% h %4 .t\CSV.dial :flush C'o  +76 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'F% h %p .t\CS.seek 7ml C'^'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z +113 h .t\CTV.dial :air .\CS.seek 0ml C'r'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'L +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'0% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C'J'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'U +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &S(ӷ +6 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'8( +416 h ( M&s& h &ӷ M&w% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&[1 +150 h 1 M&v% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:24:11CEST30-Aug-18\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:24:07CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*{% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'-& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&=% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(`% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&O% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +7 F*q% h %&K ."readyMFB"\ATV.dial :waste2 F*K % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +159 h ( M&y& h & A(W& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(e& h &  .t\SE.seek 8.71 Pucks below Top Plate A(&' +175 h ' A(& h &  M& & h & .t\Elbow.seek 2 M&3( +6 h ( M&,& h & A(B& h &  .t\Forearm.seek 2 + 300 counts M&9'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down A(6'X M&.& h & M&\ % h %; .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.seek CC M&(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &#' +91 h ' .t\CC.seek :closed C'~(W +416 h (W .tHand.open M&21 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)n+ +6 h + S) % h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +2 S)4% h % ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)@  +73 h ."airStroke"\CS.seek 0ml C'S'4 +7 h '4 ."airStroke"\CSV.dial :air C'[ @ +70 h  .t\CTV.dial :air .delay 15 seconds +5 h @ ."airStroke"\CS.seek 10ml C'v' +910 h ' +585 .t\SSV.dial :spit,avoiding: :exhaust S) +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.reconfigure SSexhale C'{  .t\SS.seek 0ml S)#.0Xd +100 C'E9 h 9Td +100 C'Z9 h 9kd +100 C'o9 h 9d +100 C'm9 h 9d +100 C'F9 h 9d +100 C'!9 h 9d +100 C'z9 h 9d +100 C'^9 h 9d +100 C'r9 h 9 d +100 C'59 h 9 d +100 C'(9 h 96d +100 C'9 h 9Ld +100 C'9 h 9bd +100 C'09 h 9xd +100 C'u9 h 9d +100 C'9 h 9d +100 C'h9 h 9d +100 C'9 h 9d +100 C'a9 h 9d +100 C'J9 h 9d +100 C'9 h 9d +100 C'U9 h 9(d +100 C'R9 h 9>d +100 C'/9 h 9Td +100 C'x9 h 9id +100 C'L9 h 9d +100 C'b9 h 9d +100 C'+9 h 9d +100 C'G9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'w9 h 9  d +100 C'49 h 9 d +100 C'}9 h 9 *d +100 C'>9 h 9 ?d +100 C'@9 h 9 Td +100 C'[9 h 9 j d +100 C's9 h 9 $d +100 C'S9 h 9 (d +100 C'9 h 9 ,d +100 C'{9 h 9 0d +100 C'=9 h 9 4d +100 C'`9 h 9 8d +100 C'9 h 9 'X +100 (@% h % M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'S @ +19 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'{ ` +19 h ` .t\AIV.dial :bypass C'= { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&v(- +166 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&K'η +91 h 'η .tHand.close M&-/ +150 h / M&W& h &- M&% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'c(W +416 h (W C'& h &W .tHand.open M&t1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%B +151 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%\ ' +1597 h ' .t\PSV.dial PSR P%P +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%9  C'&  h +75 .t\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%'  h .t\PS.seek 0ml P%6' +2610 h ' .t\PSV.dial :air P%2  +37 h  .t\PS.seek 2.5ml P%8' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%C' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&1& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'_( +416 h ( M&f& h &ӷ M&N% h % .t\Forearm.seek :retract M&%'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &<'η +91 h 'η .tHand.open M&\\1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'X& h & M&p% h %\  A(g% h %X A(I. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &  M&H& h &- M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &:( +166 h ( M&& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(M& h &  .t\SE.seek 7.71 Pucks below Top Plate A(' +185 h ' A(d& h &  M&A& h & .t\Elbow.seek 2 M&E( +7 h ( M&Z& h & A(m& h &  .t\Forearm.seek 2 + 300 counts M&F'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +150 h / ."downElevator"\SE.seek :down A(r'X M&|& h & M&]% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +93 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&!1 +150 h 1 M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'5  +151 h  .t\CSV.dial CTV C'~  +18 h  .t\CS.seek 5ml C'h'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'a'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%J  +38 h  .t\PS.seek 2.5ml P%R' +2609 h ' .t\PSV.dial :mixloop P%/ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%0  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%( +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%b  +151 h  .t\PS.seek :empty P%+' +2610 h ' .t\CBV.dial :waste1 C'G @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'w @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'}  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'U @ +38 h @ .t\CS.seek 10ml C'@' +2647 h ' .t\CSV.dial CTV C'[  +37 h  .t\CS.seek 0ml C'S'4 +2648 h '4 .t\CBV.dial :waste1 C'{ @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'=  +151 h C'`& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (x( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&z& h &ӷ M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &O( +143 h ( M&q& h & A(>& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A(T& h & .t\SE.seek 11.71 Pucks below Top Plate A(L'Ɛ +146 h 'Ɛ M&-& h & A(W& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&s, +7 h , .t\Elbow.reconfigure TightElbow A(K- M&$&.,(\ #b h -+Ɛ .t\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M& % h %Y A(v& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&e'υ +21 h 'υ M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' .t\SE.seek 13.04 Pucks below Top Plate A('" +36 h '" M&B& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +90 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&\ ' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C'&( +416 h ( A(3& h & A('& h & M&*& h &ӷ M&2% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&8& h & A("& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&?, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&1% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'υ +21 h 'υ M&7& h & A(y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(c% h %" ."downElevator"\SE.seek :down A(f'X +100 (N% h % M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +33 h 'X ."downElevator"\SE.coast A(5 +56 h '\  .t\SC.coast .\PSV.dial :air A(6 P%Q  +113 h  P%#% h % .t\PS.seek 0.499ml P%\\' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%l +38 h .t\PRV.dial :air P%%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%X  +38 h .t\CBV.dial AIV C'p  +37 h .t\PRV.dial between CBV and air P%g  +19 h  .t\PRV.dial :air P%I  +19 h  P%D% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %H' +250 h ' .t\PRV.dial :waste2 P%i @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %<' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&$1 +2 h 1 M&M& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&d(- +165 h (- M&A& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&m'η +91 h 'η .tHand.close M& / +150 h / M&^& h &- M&|% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'& h &W .tHand.open M&E1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'_& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%! @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%j  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%5  +75 h  .tdelay 2 seconds +200 P%~% h % .t\PS.seek 1.499ml P%o' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%h' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%J  +37 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %/'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%0  +75 h .tdelay 2 seconds +200 P%(% h %r .t\PS.seek 0.475ml P%b' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%R  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%G'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%w  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %}' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%U @ +75 h @ .t\PS.seek 0ml P%a' +2610 h ' .t\PSV.dial :air P%[  +75 h  .t\PS.seek 2.5ml P%@' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%{ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%=' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%`  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %z' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'4 & +50 h & .t\AWV.select :waste C' +6 P%q% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %>'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'T { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :PML P%K +37 h .t\PS.seek 0ml P% ' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'v& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'u& F* @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass F*  .t\PSV.dial PSR P%B +22 h  ."MFBPCR"delay 20 seconds +91 h P%.% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%k' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*O I +129 h I F*L% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%P' +1598 h ' .t\PSV.dial PSR P%e +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%, +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*3'ͯ +92 h P%'% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%*' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*2 % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%"  +38 h  .t\PS.seek 0ml P%\ ' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*C% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *1' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +217 ."MFBPCR"\ATV.dial :AIV F*y I +43 h I F*c% h % ."MFBPCR"\AS.seek 0.469ml F*6' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%N' +2610 h ' .t\PSV.dial PSR P%) +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%Q' +739 h ' ."MFBPCR"delay 5 seconds +500 F*#9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*V n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f% h % ."MFBPCR"\AS.seek 0.041ml F*x'% +336 h ' .t\PSV.dial PSR P%+ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%l' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial PSR P%p +113 h P%g% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%I' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%D  +37 h  .t\PS.seek 0ml P%\\' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%H' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%$ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %M'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %A' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*Z I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%^' +2610 h ' .t\PSV.dial :mixloop P%| +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%m  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *]'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*& I +42 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*E' +171 h ' .t\PSV.dial :mixloop P%r +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %_' +618 h ' ."MFBPCR"\ATV.dial :waste F*! n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*j  +86 h  F*5% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *~' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*<  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'J'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV C'h  .t\CTV.dial CSV C'0  +38 h  +113  .t\CBV.dial :waste1 C'( @ +37 h @ .t\CS.seek 0ml C'/'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*R  +22 h ."MFBPCR"delay 3 seconds +300 F*b% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'w  +37 h  C'G% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*U  +22 h F*}% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*['& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*a n +107 h n F*{% h %& ."MFBPCR"\AS.seek 0.092ml F*@'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'`' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&z' +91 h ' .tHand.close M&T/ +150 h / .t\CC.seek :open C'>( +415 h ( M&-& h &ӷ M&% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&B1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'O& h & M&L% h %\  A(% h %X A(;. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (k( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (e& h &  M&P& h &- M&t% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A('& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(' +196 h ' A(s& h &  M&2& h & ."WCR"\Elbow.seek 2 M&3( +6 h ( M&"& h & A( & h &  ."WCR"\Forearm.seek 2 + 300 counts M&C'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +58 h '&0 ."MFBPCR"delay 3 seconds +92 h / ."downElevator"\SE.seek :down A('X M&8& h & M&y% h %; ."WCR"\Forearm.seek :retract M&c'\ +91 h '\  ."WCR"\Elbow.seek CC M&\ (ӷ +104 h 'X ."downElevator"\SE.coast A(5 +13 ."MFBPCR"\ATV.dial :waste2 F*1 % +25 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &)' +18 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +73 h ' ."WCR"\CC.seek :closed C'N(W +416 h (W ."WCR"Hand.open M&1 +71 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *#' +57 h 1 M&V% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +6 h + S)+% h % C'Q& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'%  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*V% h % ."MFBPCR"\AS.seek 0.23ml F*+' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*%'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*k% h %X ."MFBPCR"\AS.seek 0.24ml F*6' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*g n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*p  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *I'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*l @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*x I +86 h I ."MFBPCR"\AS.seek 0ml F*$'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*#'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *Z'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*A% h %(A ."MFBPCR"\AS.seek 0.6ml F*|'\  +1032 h '\  F*M% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *d' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*? % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3473 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2109h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*r  +10 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +22 h ."MFBPCR"delay 2 seconds +200 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.53ml F*S' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*b  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*G'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*}  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*[  +65 h ."MFBPCR"\ARV.dial :air1 F*{  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254660 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'4  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C': @ +75 h @ +50 S)q% h % ."WCR"\CBV.dial between CSV and waste2b C'F  +94 h  C'-% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'z' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'v  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'B  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'.  +37 h  C'L% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C';' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'O @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C't  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'P ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C''  '9  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 's'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'2  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'"% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'*' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'7% h % ."WCR"\CSV.dial between air and CTV C'1 ` +57 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'3  'C  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '@' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'>  +150 h C'c& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(V% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (Q( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&+& h &ӷ M&)% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +143 h ( M&%& h & A(g& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &p' +89 h ' A(6& h & ."WCR"\SE.seek 12.71 Pucks below Top Plate A(D' +137 h ' M&X& h & A(I& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &x'Y +25 h 'Y ."WCR"\Elbow.hold M&\\, +6 h , ."WCR"\Elbow.reconfigure TightElbow A($- M&$&.,(\ #b h -+ ."WCR"\SE.seek 13.04 Pucks below Top Plate A( '" +27 h '" M&i% h %Y A(#& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&Z& h & A(A& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +22 h ' ."WCR"\SE.seek 14.04 Pucks below Top Plate A(M' +36 h ' M&|& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&?(ӷ +143 h (ӷ M&d& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&r/ +150 h / ."WCR"\CC.seek :open C'H( +416 h ( A(^& h & A(& h & M&E& h &ӷ M&!% h % ."WCR"\Forearm.seek :retract M&j'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &&( +142 h ( M&<& h & A(o& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &5'Y +93 h 'Y ."WCR"\Elbow.hold M&~, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&]% h %Y A(J& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&h'υ +20 h 'υ M&& h & A((& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +23 h ' A(S% h % ."downElevator"\SE.seek :down A(b'X +100 (w% h %v M&0% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&G1 +2 h 1 M&}& h & M&U% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.seek :garage M&{'η +91 h 'η .tHand.close M&=/ +150 h / M&a& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &:' +91 h ' .t\CC.seek :closed C'q(W +416 h (W C'F& h &W .tHand.open M&-1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'v& h &W C'% h % .t\CSV.dial CSR C'z  +38 h  C'B% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'K% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '.'[ +463 h '[ .t\CSV.dial :waste C'L +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'u'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C';& h &W C'O% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'P% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  '9  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'2'4 +766 h '4 #t\Flushing lower manifold's waste2b port C's& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'"'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +37 h @ C'*% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C',  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'y  +75 h  .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing upper manifold C'1& h &W C'8% h %4 .t\CSV.dial :flush C'3  +75 h  .t\CS.seek 5ml C'C'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'> 'c  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'V'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'+'4 +506 h '4 C')& h &W C'%% h %4 .t\CSV.dial :flush C'g  +38 h  .t\CS.seek 3ml C'6'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'X% h %0 .t\CS.seek 5ml C'I'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'i'0 +506 h '0 .t\CSV.dial :waste C'# +75 h .t\CS.seek 0ml C'f'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'Z& h &W C'A% h %4 .t\CSV.dial CSR C'|  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'p'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'k' +416 h ' ."connect"\CSV.dial CBV C'D .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'x  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'd' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'm  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'N  +38 h  +113  .t\CBV.dial :waste1 C'^ @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'E  +38 h  C'!% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C']  +37 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'S  'j +37 h +76 .t\CTV.dial :air C'&  +150 h .t\CS.seek 0ml C'w'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +506 h '0 .t\CSV.dial :waste C'/ +38 h .t\CS.seek 0ml C'G'4 +507 h '4 #t\Flushing upper manifold C'}& h &W C'U% h %4 .t\CSV.dial :flush C'5  +37 h  .t\CS.seek 5ml C'h'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +37 h @ C'% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CBV C'n .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'b'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +507 h '0 .t\CSV.dial :waste C'~ +37 h .t\CS.seek 0ml C'='4 +507 h '4 C'a& h &W C'% h %4 .t\CSV.dial :flush C'R  +37 h  .t\CS.seek 3ml C'{'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'F +76 h .t\CS.seek 0ml C'-'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'v% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +38 h @ C':% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  '  +38 h  +112  .t\CBV.dial :waste1 C'L @ +38 h @ .t\CS.seek 0ml C'.'4 +766 h '4 #t\Flushing lower manifold's waste2b port C';& h &W C'O% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'P% h %p .t\CS.seek 7ml C't'i +416 h 'i ."connect"\CSV.dial CTV C''  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'9  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'e& h &W C's% h %4 .t\CSV.dial CSR C'  +38 h  C' % h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'2'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'W' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'* '"  +38 h +75 .t\CTV.dial :intake C',  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'y' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +606 h '4 .t\CSV.dial CSR C'8  +113 h  C'3% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'7'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'C'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &>(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&)& h &ӷ M&%% h % .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &+(- +264 h (- M&\ & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&I1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&Z1 +2 h 1 M&A& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &|(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.seek :garage M&p'η +91 h 'η .tHand.close M&D/ +150 h / M&x& h &- M&k% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'& h &W .tHand.open M&N1 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'!% h %4 .t\CSV.dial CSR C'  +113 h  C'<% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '?% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'o'[ +463 h '[ .t\CSV.dial :waste C'] +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +2009 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& +75 h .t\CTV.dial :intake C'(  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'w'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'/  +75 h #t\Flushing upper manifold C'0& h &W C'}% h %4 .t\CSV.dial :flush C'U  +75 h  .t\CS.seek 5ml C'5'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +37 h @ C'M% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h +113 h .t\CTV.dial :air .\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +507 h '0 .t\CSV.dial :waste C'b +37 h .t\CS.seek 0ml C'~'4 +507 h '4 C'Q& h &W C'a% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'R'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'{'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'F'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&H& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&B/ +150 h / .t\CC.seek :open C':( +416 h ( M&z& h &ӷ M&K% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &L(- +264 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:01 #\Preparing to sleep until Sun Sep 23 06:01:01 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST23-Sep-18 #\Continuing at 06:01CEST23-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:25:38CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST23-Sep-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2109h25ml.pcr F*P% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F*9 % +86 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +65 h  F*8% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*)% h % ."MFBPCR"\AS.seek 0.81ml F*%'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*I% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*g  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*|'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*<  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*C' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*?  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*]' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*o  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*( n +21 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*/'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*M% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2111h25ml.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*[  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*=% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*''m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*3  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *q'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*) I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*T% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*\ n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*>' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +108 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*Z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*A n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.65ml F*x' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*k  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*p% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2113h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*m  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*N% h %Z ."MFBPCR"\AS.seek 0.81ml F*<'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +65 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*/  +32 h  ."MFBPCR"\ATV.dial :air3 F*w % +86 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*b  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*H  +10 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*;  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *@'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*O% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*P I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*9 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*''< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +107 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2116h25ml.pcr F*8% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*I  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*X  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *$'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +21 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*o  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*/  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*w  +22 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *}'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*U n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*M' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*l  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*[  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*5'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*b  +22 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*~'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*= n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*v% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2118h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*z  +22 h ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*9  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F**  +32 h  ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +21 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*3'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*V  +11 h  F*$% h %V ."MFBPCR"\AS.seek 0.5ml F*i'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +21 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*p I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*# % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*m  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*^% h %< ."MFBPCR"\AS.seek 0.594ml F*!'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*N n +21 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*?  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*J % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*] @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*(  +151 h F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b */' +424 h ' ."MFBPCR"\ATV.dial :ASV F*w n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*G  +43 h  F*0% h % ."MFBPCR"\AS.seek 0.62ml F*U'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*h n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *l'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*n'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*M % +43 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*5% h % ."MFBPCR"\AS.seek 0.71ml F*=' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*~% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*R% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*{ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*v% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*B% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*r' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*;'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*O% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*-' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*P n +86 h n ."MFBPCR"\ASV.dial :wasteR F*`  +22 h  ."MFBPCR"\AS.seek 0ml F*@'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*.% h %V ."MFBPCR"\AS.seek 0.01ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*'% h % ."MFBPCR"\AS.seek 0.58ml F*e' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h % q ."MFBPCR"\AS.seek 0.605ml F**'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*"% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *8'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*3  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *W'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*7 n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +202 h 'R ."MFBPCR"\SPE.coast F*4  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*)  +43 h  F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*\ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*I  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*$  +65 h  F*X% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *i'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*A n +65 h n F*g% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*D I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Z  +42 h  F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *x': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*6  +64 h  F*#% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *|'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*+ n +65 h n F*m% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*^  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*N n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *E'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*! n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*<  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*o'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*S' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*&% h % ."MFBPCR"\AS.seek 0.97ml F*j'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*w I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *0'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *}'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*l  +43 h  F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *n' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*Q' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +129 h n ."MFBPCR"\AS.seek 0ml F*5'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*b% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*~  +21 h ."MFBPCR"\ATV.dial :air3 F*= % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F*v'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +65 h  F*z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*- % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*2  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' @CEST1537567247.20 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *W'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*c % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537675261.78 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )) h  C' 'T h  P% % h  F* *\  h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *%% h %V .t\AS.configure ASconfig S)I% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'>& S)#.0X 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:26:35CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #6) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'&& h & .tHand.open M&S1 +150 h 1 M&w& h &_ M&j% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &G(- +165 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &h'η +91 h 'η .tHand.close M&U/ +150 h / M&l& h &- M&M% h %η .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &a' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'Q& h &W .tHand.open M&51 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +150 h  C'{% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'R'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'H'[ +463 h '[ .t\CSV.dial :waste C'v +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'B& h &W C'% h %4 .t\CSV.dial :flush C'\\  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'z .t\CBV.dial CSV C'd  h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'r  +76 h #t\Flushing upper manifold C'& h &W C'O% h %4 .t\CSV.dial :flush C';  +75 h  .t\CS.seek 5ml C'`'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'@% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'''4 +507 h '4 C't& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C's'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +37 h @ C'e% h %0 .t\CS.seek 5ml C'"'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'*'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +506 h '0 .t\CSV.dial :waste C', +76 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +7 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'4( +416 h ( M&7& h &ӷ M&c% h % .t\Forearm.seek :retract M&W'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &:'η +91 h 'η .tHand.open M&1 +150 h 1 M&_% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:26:40CEST30-Aug-18\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:26:35CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C']& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&)% h %\  A(T% h %X A(. h ., A(\ & h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&%& h &- M&I% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &>( +6 F*q% h %&K ."readyMFB"\ATV.dial :waste2 F*$ % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *X' +160 h ( M&V& h & A(A& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &g' +89 h ' A(i& h &  .t\SE.seek 5.71 Pucks below Top Plate A(D' +205 h ' A(& h &  M&f& h & .t\Elbow.seek 2 M&Z( +7 h ( M&p& h & A(#& h &  .t\Forearm.seek 2 + 300 counts M&k'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down A(6'X M&m& h & M&|% h %; .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&!(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +28 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &?' +91 h ' .t\CC.seek :closed C'J(W +416 h (W .tHand.open M&1 +150 h 1 M&x% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'&& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +150 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S)g +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'O9 h 9Td +100 C'9 h 9kd +100 C';9 h 9d +100 C'9 h 9d +100 C'@9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'P9 h 9d +100 C'.9 h 9 d +100 C'9 h 9 d +100 C'9 h 96d +100 C'99 h 9Ld +100 C't9 h 9bd +100 C'9 h 9xd +100 C'-9 h 9d +100 C''9 h 9d +100 C'29 h 9d +100 C'e9 h 9d +100 C' 9 h 9d +100 C's9 h 9d +100 C'*9 h 9d +100 C',9 h 9(d +100 C'9 h 9>d +100 C'"9 h 9Td +100 C'89 h 9id +100 C'u9 h 9d +100 C'39 h 9d +100 C'y9 h 9d +100 C' 9 h 9d +100 C'=9 h 9d +100 C'b9 h 9d +100 C'c9 h 9  d +100 C'9 h 9 d +100 C'[9 h 9 *d +100 C'L9 h 9 ?d +100 C'F9 h 9 Td +100 C'9 h 9 j d +100 C'79 h 9 $d +100 C'19 h 9 (d +100 C'W9 h 9 ,d +100 C'9 h 9 0d +100 C')9 h 9 4d +100 C'T9 h 9 8d +100 C'9 h 9 9 h 9 d +100 C'g9 h 9 d +100 C'|9 h 9 d +100 C'9 h 9 d +100 C'^9 h 9 d +100 C'k9 h 9 d +100 C'D9 h 9 1d +100 C'Z9 h 9 Dd +100 C'9 h 9 Xd +100 C'69 h 9 kd +100 C'9 h 9 ~d +100 C'49 h 9 d +100 C'9 h 9 d +100 C'&9 h 9 d .t\CH.hold 5:00,85C C'x   P h  +30000  C'<9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'?'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'N @ +37 h @ .t\CS.seek 5ml C' 'p +1373 h 'p .t\CSV.dial CTV C'0  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'/ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'S  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'l @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'X'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'M +113 h .t\CBV.dial CSV C'  +19 h C'U% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'n  +38 h C'G% h %p .t\CS.seek 7ml C'C'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'( @ +38 h @ .t\CS.seek 10ml C'Q' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'5 ` +132 h ` #tLysing Complete C'~& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&% h %\  A(}% h %X A({. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (a( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&v& h & A(H& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &B' +90 h ' A(& h & .t\SE.seek 13.71 Pucks below Top Plate A(\\'n +125 h 'n M&R& h & A(d& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &z'Y +25 h 'Y .t\Elbow.hold M&r, +7 h , .t\Elbow.reconfigure TightElbow A(K- h -+n M&$&.,(\ #b .t\SE.seek 14.04 Pucks below Top Plate A( ' +27 h ' M&O% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&;'υ +21 h 'υ M&& h & A(@& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &`' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(' +36 h ' M&P& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&-/ +150 h / .t\CC.seek :open C''( +416 h ( A(2& h & A(e& h & M& & h &ӷ M&s% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &,( +143 h ( M&& h & A("& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &8'Y +92 h 'Y .t\Elbow.hold M&u, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &31 +150 h 1 M&y% h %Y A( & h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&='υ +21 h 'υ M&b& h & A(c& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A([% h % ."downElevator"\SE.seek :down A(L'X +100 (F% h %- M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'1 @ +19 h @ .t\CS.seek 10ml C'W' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C') { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'T& h & .tHand.open M&1 +2 h 1 M&\ & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&_(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&$'η +90 h 'η .tHand.close M&]/ +150 h / M&A& h &- M&:% h %η .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +265 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'#(W +416 h (W C'& h &W .tHand.open M&+1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +150 h P%m% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%|' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%^' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%D  C'k  h +76 .t\PSV.dial :mixloop P%Z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%6' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%4' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%&' +2610 h ' .t\PSV.dial :air P%x  +38 h  .t\PS.seek 2.5ml P%<' +2609 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&N& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +6 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C' ( +416 h ( M&M& h &ӷ M&% h % .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &X(- +264 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&(1 +150 h 1 M&w% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'5& h & M&~% h %\  A(% h %X A(}. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ({( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &v( +166 h ( M&H& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &!' +90 h ' A(R& h &  .t\SE.seek 4.71 Pucks below Top Plate A(d' +214 h ' A(K& h &  M&O& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A(@& h &  .t\Forearm.seek 2 + 300 counts M& '; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &P/ +150 h / ."downElevator"\SE.seek :down M&.& A(B'X h & M&% h %; .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.seek CC M&z(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +19 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&`1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'J  +19 h  .t\CS.seek 5ml C'2'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'e' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%s  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%" +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%3  +151 h  .t\PS.seek :empty P%y' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'b  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'c @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'[  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'* ` +19 h ` .t\CSV.dial :air C', @ +38 h @ .t\CS.seek 10ml C'F' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'1'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C')  +150 h C'T& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(\ % h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (8( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (=& h & M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &I( +143 h ( M&q& h & A(L& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &a' +89 h ' A(Q& h & .t\SE.seek 14.71 Pucks below Top Plate A(u'] +116 h '] M&]& h & A(A& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &:'Y +26 h 'Y .t\Elbow.hold M&7, +6 h , .t\Elbow.reconfigure TightElbow A($- M&$&.,(\ #b h -+] .t\SE.seek 15.04 Pucks below Top Plate A(Y' +27 h ' M&f% h %Y A(_& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&i'υ +20 h 'υ M&'& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &+' +22 h ' .t\SE.seek 16.04 Pucks below Top Plate A(%' +37 h ' M&p& h & M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&g(ӷ +143 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&|' +90 h ' .tHand.close M&D/ +150 h / .t\CC.seek :open C'k( +416 h ( A(Z& h & A(& h & M&^& h &ӷ M&% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&4& h & A(x& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &&'Y +92 h 'Y .t\Elbow.hold M&j, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &<1 +150 h 1 M&N% h %Y A(0& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &/'υ +21 h 'υ M&l& h & A(V& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &E' +22 h ' A(#% h % ."downElevator"\SE.seek :down A(M'X +100 (% h %X M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast .\PSV.dial :air A(6 P%G  +113 h  P%?% h % .t\PS.seek 0.499ml P%(' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%w +38 h .t\PRV.dial :air P%U  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%5  +37 h .t\CBV.dial AIV C'~  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%}  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&1 +2 h 1 M&R& h & M&X% h %\  .t\Elbow.seek FlushPuck::Garage M&C(- +166 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&@/ +150 h / M&P& h &- M&.% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +264 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'd& h &W .tHand.open M&1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  C' & h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%` @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%r  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%J% h % .t\PS.seek 1.499ml P%;' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%{' +1094 h ' .t\PSV.dial :air P%W  +38 h  .t\PS.seek 0ml P%2' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%e +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%s  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %"'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%3' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%b  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%c  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %[' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%* +75 h .t\PTV.dial :PRV,avoiding: :puck P%, @ +76 h @ .t\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%F' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%)' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%T  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%\ +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%=% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%q% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %L'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'Q { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %z' +1313 h ' .t\PSV.dial :air P%]  +37 h  .t\PS.seek 2.5ml P%A' +2610 h ' .t\PSV.dial :PML P%$ +38 h .t\PS.seek 0ml P%f' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '_& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C''& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*-  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +21 h  ."MFBPCR"delay 20 seconds +92 h P%m% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*I I +129 h I F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *:'6 +66 .t\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%7' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*D  +22 h ' .t\PSV.dial PSR P% +21 h  F* % h %6 ."MFBPCR"\AS.seek 0.329ml F*Z'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%^' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%x  +37 h  .t\PS.seek 0ml P%|' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*g  +22 h  F*<% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *N' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%0 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%l' +217 ."MFBPCR"\ATV.dial :AIV F*V I +42 h I F*#% h % ."MFBPCR"\AS.seek 0.469ml F*6' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%S +113 h P%&% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%/' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +38 h  .t\PS.seek 0ml P%G' +739 h ' ."MFBPCR"delay 5 seconds +500 F*?9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*E n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*M% h % ."MFBPCR"\AS.seek 0.041ml F*8'% +336 h ' .t\PSV.dial PSR P%y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%w' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%5' +2610 h ' .t\PSV.dial PSR P%~ +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%}' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%(' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %R'k +2679 h 'k .t\PSV.dial :waste1 P%X  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %K' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*O I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *j'V +850 h ' .t\PSV.dial :air P%@  +113 h  .t\PS.seek 2.5ml P%P' +2609 h ' .t\PSV.dial :mixloop P%. +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %n' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %!' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*9  +43 h  F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*k I +43 h I F*d% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%B +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb % ' +619 h ' ."MFBPCR"\ATV.dial :waste F*` n +129 h n ."MFBPCR"\AS.seek :empty F*t'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*r  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *J' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*;% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *W'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'{  +38 h  C'e% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C's'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'2  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'"'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*3% h %_ ."MFBPCR"\AS.seek 0.434ml F*b'ݏ +110 h '4 .t\CSV.dial CSR C'c  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'*'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*,  +22 h F*[% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*F'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'T' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'\ '4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'L( +416 h ( M&]& h &ӷ M&z% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +264 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &-'η +91 h 'η .tHand.open M&p1 +150 h 1 M&m% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'I& h & M&u% h %\  A(>% h %X A(h. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (a( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (i& h &  M&& h &- M&+% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +166 h ( M&D& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &  ."WCR"\SE.seek 3.71 Pucks below Top Plate A(:' +223 h ' A(7& h &  M&& h & ."WCR"\Elbow.seek 2 M&Z( +7 h ( M&x& h & A(g& h &  ."WCR"\Forearm.seek 2 + 300 counts M&<'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +31 h '&0 ."MFBPCR"delay 3 seconds +119 h / ."downElevator"\SE.seek :down A(4'X M&0& h & M&V% h %; ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.seek CC M&|(ӷ +90 ."MFBPCR"\ATV.dial :waste2 F*N % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&l& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +91 h ' ."WCR"\CC.seek :closed C'(W +415 h (W ."WCR"Hand.open M&%1 +43 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *?' +85 h 1 M&E% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +7 h + S)y% h % C'G& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'U  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*h % +43 h % F*i% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*-% h % ."MFBPCR"\AS.seek 0.579ml F*D' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*A'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*7% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*x n +86 h n ."MFBPCR"\ASV.dial :wasteR F*g  +43 h  ."MFBPCR"\AS.seek 0ml F*^'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *V'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*Y % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*N' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*l n +108 h n ."MFBPCR"\ASV.dial :wasteR F*S% h %  ."MFBPCR"\AS.seek 0.09ml F*<' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*Z I +86 h I F*F% h % ."MFBPCR"\AS.seek 0.12ml F*4' +625 h ' ."MFBPCR"\ATV.dial :air3 F*# % +21 h % F*L% h % ."MFBPCR"\AS.seek 0.13ml F*/' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*E% h % ."MFBPCR"\AS.seek 0.23ml F*y' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*U'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*a% h %X ."MFBPCR"\AS.seek 0.24ml F*6' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*~  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *}'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*w @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*8 I +85 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*?'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*X  +42 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *O'\  +1032 h '\  F*H% h %\  ."MFBPCR"\AS.seek 0.5ml F*@'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*K% h %(A ."MFBPCR"\AS.seek 0.6ml F*.'\  +1031 h '\  F*R% h %\  ."MFBPCR"\AS.seek 0.5ml F*n'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*9% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *C' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*j % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *P'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*d  +64 h  ."MFBPCR"\ARV.dial :carrier F*B  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*!'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*J  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*e% h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*s  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.53ml F*1' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +107 h  ."MFBPCR"delay 7 seconds +700 F*c% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*[  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*)% h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*= % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254689 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'v @ +75 h @ +50 S)q% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C'\\ ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'_  ."WCR"\CSV.dial CTV C'z  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'p  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ''' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'$  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'm  +38 h  C'u% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'h' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'I @ +75 h @ +50 S)>% h % ."WCR"\CBV.dial between CSV and waste2b C'+  +94 h  C'-% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'D  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'f'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'7  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'A' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'x  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'g  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'^' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'Y @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'V  +94 h  C'l% h % ."WCR"\CSV.dial between air and CTV C'S ` +56 h ` ."WCR"\CS.seek 7.288ml C'N' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  'Z  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'F'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'<  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '#' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'L  +151 h C'4& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&%% h %\  A(E% h %X A(/. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (G( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&y& h &ӷ M&&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &a( +142 h ( M&U& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +90 h ' A(6& h & ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +106 h 'L M&5& h & A(}& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &8'Y +26 h 'Y ."WCR"\Elbow.hold M&(, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+L ."WCR"\SE.seek 16.04 Pucks below Top Plate A(X' +27 h ' M&% h %Y A(?& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&H'υ +20 h 'υ M&O& h & A(K& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &@' +23 h ' ."WCR"\SE.seek 17.04 Pucks below Top Plate A(R' +36 h ' M&.& h & M&9% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +89 h '\  ."WCR"\Elbow.seek CC M&j(ӷ +143 h (ӷ M&C& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&d' +91 h ' ."WCR"Hand.close M&B/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(P& h & A(!& h & M&& h &ӷ M&`% h % ."WCR"\Forearm.seek :retract M&r'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +143 h ( M&& h & A(;& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +92 h 'Y ."WCR"\Elbow.hold M&J, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &e1 +150 h 1 M&% h %Y A(s& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&2'υ +20 h 'υ M&{& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(1% h % ."downElevator"\SE.seek :down A(3'X +93 h 'X ."downElevator"\SE.coast A(5 +7 (c% h %X M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'"& h & .tHand.open M& 1 +2 h 1 M&[& h & M&,% h %\  .t\Elbow.seek FlushPuck::Garage M&W(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&)/ +150 h / M& & h &- M&*% h %η .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'q(W +416 h (W C' & h &W .tHand.open M&]1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'z& h &W C'_% h % .t\CSV.dial CSR C'  +37 h  C'p% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '$% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'm'[ +463 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'h& h &W C'I% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '+'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'% h %p .t\CS.seek 7ml C'i'i +416 h 'i ."connect"\CSV.dial CTV C'D  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'f& h &W C'x% h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +38 h @ C'^% h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'V  +75 h  .t\CS.seek 0ml C'l'4 +766 h '4 #t\Flushing upper manifold C'S& h &W C'0% h %4 .t\CSV.dial :flush C':  +75 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C'<% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CBV C'L .t\CBV.dial CSV C'4  +38 h +75 .t\CTV.dial :air C'%  +151 h .t\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'y'4 +506 h '4 C'&& h &W C'U% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'6'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'5% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'? +75 h .t\CS.seek 0ml C'O'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'K& h &W C'M% h %4 .t\CSV.dial CSR C'.  +150 h  C'9% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'~'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'a' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'8  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'C' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'X  +113 h  C'@% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'('P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'B' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'n  +38 h  +113  .t\CBV.dial :waste1 C'P @ +37 h @ .t\CS.seek 0ml C'!'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'`% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ';' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  +38 h  .t\CBV.dial :waste2b C'j  +75 h  .t\CS.seek 0ml C'e'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '{'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'1  'r +38 h +75 .t\CTV.dial :air C'k  +151 h .t\CS.seek 0ml C'c'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'" +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C'[& h &W C',% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'2'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'b% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +506 h '0 .t\CSV.dial :waste C'J +38 h .t\CS.seek 0ml C')'4 +507 h '4 C' & h &W C'*% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'\ % h %0 .t\CS.seek 5ml C'W'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'='4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'\\& h &W C'z% h %4 .t\CSV.dial :flush C'_  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +38 h @ C'v% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '  +37 h  +113  .t\CBV.dial :waste1 C'u @ +38 h @ .t\CS.seek 0ml C'm'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'h& h &W C'I% h %4 .t\CSV.dial :flush C'>  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +37 h @ C'% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #tDisinfecting external Intake with kill C'i& h &W C'f% h %4 .t\CSV.dial CSR C'x  +38 h  C'g% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'7'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'A' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'^ '  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'V' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Y  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'S'4 +607 h '4 .t\CSV.dial CSR C'0  +113 h  C':% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'N' +1372 h ' .t\CSV.dial CBV C'< +113 h .t\CS.seek 0ml C'Z'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&4& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +7 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M&E' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'/( +416 h ( M&&& h &ӷ M&U% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +265 h (- M&|& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&}1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&K1 +2 h 1 M&M& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &.(- +6 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&/ +150 h / M&8& h &- M&a% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +265 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +90 h ' .t\CC.seek :closed C'((W +416 h (W C'& h &W .tHand.open M&n1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'`% h %4 .t\CSV.dial CSR C'!  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ';'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 's'4 +2009 h '4 #t\Purging external Intake C'e& h &W C'{% h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'c'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'"  +75 h #t\Flushing upper manifold C'& h &W C'[% h %4 .t\CSV.dial :flush C',  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'R% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'2 +113 h .t\CTV.dial :air .\CS.seek 0ml C'3'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'J'4 +507 h '4 C'G& h &W C' % h %4 .t\CSV.dial :flush C'*  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'% h %0 .t\CS.seek 5ml C'\ 'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C'= +76 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +7 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&p/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&$% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &I'η +91 h 'η .tHand.open M&>1 +150 h 1 M&m% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:17 #\Preparing to sleep until Tue Sep 25 06:01:17 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST25-Sep-18 #\Continuing at 06:01CEST25-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:28:06CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST25-Sep-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2309h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*D  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *i'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +21 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +65 h  F*0% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *:'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*U'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*X  +11 h  F*@% h %V ."MFBPCR"\AS.seek 0.5ml F*.'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *!'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*j  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*;  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*s% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.6ml F*"'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*c  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*2  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2311h25ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*G % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*W% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*_  +65 h  F*p% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *C'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +32 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*f  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*A  +43 h  ."MFBPCR"\ARV.dial :carrier F*:  +10 h  F*S% h %V ."MFBPCR"\AS.seek 0.5ml F*l'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *q'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*& I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*Q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*| n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*L' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*}  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*O'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*8' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*a  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*~% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2313h25ml.pcr F*.% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +21 h F*n% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*1% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *s'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*"  +32 h  ."MFBPCR"\ATV.dial :air3 F*c % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *_'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*C% h %V ."MFBPCR"\AS.seek 0.5ml F*B'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *#'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*'% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*A'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*S % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2316h25ml.pcr F*0% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*N  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F*E'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*}  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +64 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*O % +86 h % F*a% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*8% h % ."MFBPCR"\AS.seek 0.81ml F*@'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ARV.dial :carrier F*;  +11 h  F*s% h %V ."MFBPCR"\AS.seek 0.5ml F*{'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*r n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*"  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*c  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *['Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*, n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*w  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +22 h n ."MFBPCR"delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*W  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2318h25ml.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *h'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*#'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*T% h %n ."MFBPCR"\AS.seek 0.82ml F*m'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *f'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +31 h  ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +21 h % ."MFBPCR"delay 3 seconds +300 F*0% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ARV.dial :carrier F*E  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +22 h ."MFBPCR"delay 2 seconds +200 F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*~ I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*8% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*? % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*@' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +21 h  ."MFBPCR"delay 7 seconds +700 F*P% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*!  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*e% h % \\ ."MFBPCR"\AS.seek 0.326ml F*1'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*s % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*{  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *"' +424 h ' ."MFBPCR"\ATV.dial :ASV F*c n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*,'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*2 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *['R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *w'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*3'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *G' +424 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*)' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h % H ."MFBPCR"\AS.seek 0.735ml F*\ ' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*W' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*z% h % zG ."MFBPCR"\AS.seek 0.365ml F*=' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*_ % +43 h % F*p% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*$% h % ."MFBPCR"\AS.seek 0.579ml F*B' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %  ."MFBPCR"\AS.seek 0.604ml F*h'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*I% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*]' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*T  +21 h  ."MFBPCR"\AS.seek 0ml F*#'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*m% h %V ."MFBPCR"\AS.seek 0.01ml F*f' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +21 h F*D% h % ."MFBPCR"\AS.seek 0.58ml F*i' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h % q ."MFBPCR"\AS.seek 0.605ml F*^'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*g' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*V n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*Y'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *0'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*:  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *N' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *A'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*l n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +201 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*%'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*&  +43 h  F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *F' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*| I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*}  +43 h  F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *E': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*M n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *9' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*K  +43 h  F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *8': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*?% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *.'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*y n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*@ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*P  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *X': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*n n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.seek 0ml F*!'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*H % +43 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *j' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*d% h % ."MFBPCR"\AS.seek 0.96ml F*;'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*{% h %0 ."MFBPCR"\AS.seek 0.985ml F*1' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*k% h % ."MFBPCR"\AS.seek 0.97ml F*r'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*c I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *2'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *['R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*w  +43 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *3' +9988 h ' ."MFBPCR"\ATV.dial AIV F*b I +43 h I ."MFBPCR"\AS.seek 0.02ml F*G' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*) % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*='n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*C'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*T  +32 h  ."MFBPCR"\ATV.dial :air3 F*] % +86 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*f'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*x% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds @CEST1537740002.88 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *A'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*4 % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2548 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1537848077.22 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )& h  C' 'Q h  P% %% h  F* *| h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *F  h  .t\SPE.configure SPEconfig F*\ 2 *U% h %V .t\AS.configure ASconfig S)}% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'L& S)#.0X 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:29:03CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #7) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'k& h & .tHand.open M&11 +150 h 1 M&c& h &_ M&r% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &2'η +91 h 'η .tHand.close M&,/ +150 h / M&w& h &- M&R% h %η .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'G& h &W .tHand.open M&1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'*% h %4 .t\CSV.dial CSR C'\  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'z +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '_'4 +2010 h '4 #t\Purging external Intake C'p& h &W C'=% h %4 .t\CSV.dial :flush C'(  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '$' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'C  h +75 .t\CTV.dial :intake C'\\  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'>'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'B  +75 h #t\Flushing upper manifold C'& h &W C'I% h %4 .t\CSV.dial :flush C'h  +75 h  .t\CS.seek 5ml C'T'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'#% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'' +37 h .t\CS.seek 0ml C'D'4 +507 h '4 C'+& h &W C' % h %4 .t\CSV.dial :flush C'x  +38 h  .t\CS.seek 3ml C'f'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'i% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'^'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'g'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &S(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'( +416 h ( M&l& h &ӷ M&4% h % .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &v'η +91 h 'η .tHand.open M&t1 +150 h 1 M&o% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:29:07CEST30-Aug-18\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:29:03CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F* % #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(Q% h %X A(%. h ., A(|& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (F& h &  M&U& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +6 F*q% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *5' +160 h ( M&E& h & A(M& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') A(9& h &  M&O& h & .t\Elbow.seek 2 M&K( +7 h ( M&~& h & A(a& h &  .t\Forearm.seek 2 + 300 counts M&?'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +150 h / ."downElevator"\SE.seek :down M&& A(6'X h & M&.% h %; .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.seek CC M&`(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &j' +2 h 'X ."downElevator"\SE.coast A(5 +89 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&{1 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)H+ +7 h + S)!% h % C'k& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  .t\SS.seek 0ml S)]' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)#  +144 h  +7  S)T% h % .t\CBV.dial between SSV and waste2 C'  +18 h  C'% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'm  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '''4 +2610 h '4 C'% h %4 S)+% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C' @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'I9 h 9Td +100 C'\\9 h 9kd +100 C'h9 h 9d +100 C'>9 h 9d +100 C'#9 h 9d +100 C'T9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'm9 h 9 d +100 C'_9 h 9 d +100 C'9 h 96d +100 C'9 h 9Ld +100 C'+9 h 9bd +100 C' 9 h 9xd +100 C']9 h 9d +100 C'D9 h 9d +100 C'79 h 9d +100 C'i9 h 9d +100 C'x9 h 9d +100 C'f9 h 9d +100 C'^9 h 9d +100 C'9 h 9(d +100 C'Y9 h 9>d +100 C'9 h 9Td +100 C'09 h 9id +100 C''9 h 9d +100 C':9 h 9d +100 C'V9 h 9d +100 C'N9 h 9d +100 C')9 h 9d +100 C'9 h 9d +100 C'49 h 9  d +100 C' 9 h 9 d +100 C'9 h 9 *d +100 C'<9 h 9 ?d +100 C'-9 h 9 Td +100 C't9 h 9 j d +100 C'l9 h 9 $d +100 C'S9 h 9 (d +100 C'A9 h 9 ,d +100 C' 9 h 9 0d +100 C'&9 h 9 4d +100 C'Q9 h 9 8d +100 C'%9 h 9 & h & A(#& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &T' +23 h ' .t\SE.seek 18.04 Pucks below Top Plate A(' +36 h ' M&& h & M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'D( +416 h ( A(7& h & A(i& h & M&x& h &ӷ M&f% h % .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&Y& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &0'Y +93 h 'Y .t\Elbow.hold M&', +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &:1 +150 h 1 M&V% h %Y A(N& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&)'υ +20 h 'υ M&& h & A(4& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(<'X +84 h 'X ."downElevator"\SE.coast A(5 +16 (-% h %X M&t% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'S @ +19 h @ .t\CS.seek 10ml C'A' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'& { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Q& h & .tHand.open M&%1 +2 h 1 M&|& h & M&F% h %\  .t\Elbow.seek FlushPuck::Garage M&o(- +166 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&M& h &- M&v% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +264 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'a(W +416 h (W C'& h &W .tHand.open M&y1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%~ +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%L' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%.' +1597 h ' .t\PSV.dial PSR P%e +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%?  C'  h +75 .t\PSV.dial :mixloop P%K +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%6  h .t\PS.seek 0ml P%P' +2610 h ' .t\PSV.dial :air P%{  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%! +38 h .t\PS.seek 0ml P%k' +2609 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P%r +37 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&1' +90 h ' .tHand.close M&w/ +150 h / .t\CC.seek :open C'@( +416 h ( M&R& h &ӷ M&d% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +265 h (- M&3& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +91 h 'η .tHand.open M&1 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'& h & M&J% h %\  A(% h %X A([. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (/& h &  M&W& h &- M&*% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +166 h ( M&& h & A(=& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &`' +89 h ' A(& h &  .t\SE.seek 1.71 Pucks below Top Plate A(C': +244 h ': A($& h &  M&I& h & .t\Elbow.seek 2 M&\\( +7 h ( M&>& h & A(#& h &  .t\Forearm.seek 2 + 300 counts M&X'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&p& A(m'X h & M&\ % h %; .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'g(W +416 h (W .tHand.open M&T1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'_  +151 h  .t\CSV.dial CTV C's  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'i' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'x'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%f  +38 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%:  +150 h  .t\PS.seek :empty P%V' +2610 h ' .t\CBV.dial :waste1 C'N @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'4 @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'^ ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'-' +2647 h ' .t\CSV.dial CTV C't  +38 h  .t\CS.seek 0ml C'S'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'&  +150 h C'Q& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&%% h %\  A(|% h %X A(F. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (0( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ()& h & M&& h &ӷ M&<% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&}& h & A(q& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(G& h & .t\SE.seek 17.71 Pucks below Top Plate A(''* +87 h '* M&& h & A(M& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &v'Y +25 h 'Y .t\Elbow.hold M&l, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+* .t\SE.seek 18.04 Pucks below Top Plate A(u' +26 h ' M&9% h %Y A(o& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&D& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' .t\SE.seek 19.04 Pucks below Top Plate A(U' +36 h ' M&~& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'( +416 h ( A(K& h & A(6& h & M&& h &ӷ M&{% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &!( +142 h ( M&& h & A(8& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &k'Y +93 h 'Y .t\Elbow.hold M&r, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &n1 +150 h 1 M&% h %Y A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &"'υ +21 h 'υ M&w& h & A(E& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(a% h % ."downElevator"\SE.seek :down A(R'X +73 h 'X ."downElevator"\SE.coast A(5 +27 (d% h %X M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%j% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%c +37 h .t\PRV.dial :air P%,  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'J  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%[  +19 h  P%/% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %W' +250 h ' .t\PRV.dial :waste2 P%* @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %b' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&=1 +2 h 1 M&& h & M&5% h %\  .t\Elbow.seek FlushPuck::Garage M&Z(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.seek :garage M&>'η +91 h 'η .tHand.close M&#/ +150 h / M&& h &- M&p% h %η .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &z(ӷ +265 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'X& h &W .tHand.open M&\\1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  C'@& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%T @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%B  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%_  +75 h  .tdelay 2 seconds +200 P%s% h % .t\PS.seek 1.499ml P%h' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%A  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%i +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%f  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%:' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%Y  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%N'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%4  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%^ +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%x' +2610 h ' .t\PSV.dial :air P%t  +75 h  .t\PS.seek 2.5ml P%-' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%&' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%Q  +75 h  .t\PS.seek 2.5ml P%%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%| +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%F  +76 h P%)% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'< & +50 h & .t\AWV.select :waste C' +6 P%}% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %q'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'G { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%M' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%9' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'o& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'D& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F* @@T .t\AIV.dial :bypass F*]  .t\PSV.dial PSR P%~ +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P% ' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*'% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *v'6 +66 .t\PSV.dial :waste1 P%L  +37 h  .t\PS.seek 0ml P%e' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%u' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%l' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*?  +22 h ' .t\PSV.dial PSR P%O +21 h  F*g% h %6 ."MFBPCR"\AS.seek 0.329ml F*K'ͯ +92 h P%6% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*{ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +38 h  .t\PS.seek 0ml P%.' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*n% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%w' +217 ."MFBPCR"\ATV.dial :AIV F*E I +43 h I F*a% h % ."MFBPCR"\AS.seek 0.469ml F*P' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%! +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +2609 h ' .t\PSV.dial PSR P%1 +113 h P%k% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%"' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +37 h  .t\PS.seek 0ml P% ' +740 h ' ."MFBPCR"delay 5 seconds +500 F*j9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*R n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*0'% +336 h ' .t\PSV.dial PSR P%V +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%c' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%J +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%/  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%W' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%= +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%5  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %$' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*I I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *r'V +849 h ' .t\PSV.dial :air P%#  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%p +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %3' +5107 h ' .t\PSV.dial :air P%>  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %`' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*  +42 h  F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *\ 'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*X% h %Q ."MFBPCR"\AS.seek 0.01ml F*\\' +171 h ' .t\PSV.dial :mixloop P%( +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %@' +618 h ' ."MFBPCR"\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.seek :empty F*+'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*B  +86 h  F*_% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *s' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*b  +43 h F*h% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *A'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'f'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*Y  +22 h ."MFBPCR"delay 3 seconds +300 F*:% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +109 h '4 .t\CSV.dial CSR C'4  +38 h  C'N% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'^'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*t'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*x n +108 h n F* % h %& ."MFBPCR"\AS.seek 0.092ml F*-'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'Q' +1625 h ' ."connect"\CSV.dial CTV C'&  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'|'4 +2647 h '4 C'F& h &W #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'q( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +265 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &]'η +91 h 'η .tHand.open M&~1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M&'% h %\  A(L% h %X A(2. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ( ( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&e& h &- M&y% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &u( +166 h ( M&?& h & A(g& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &O' +89 h ' A(6& h &  ."WCR"\SE.seek 0.71 Pucks below Top Plate A(v'K +254 h 'K A(l& h &  M&{& h & ."WCR"\Elbow.seek 2 M&K( +6 h ( M&8& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&n'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ + h '&0 ."MFBPCR"delay 3 seconds +149 h / ."downElevator"\SE.seek :down M&& A('X h & M&E% h %; ."WCR"\Forearm.seek :retract M&a'\ +91 h '\  ."WCR"\Elbow.seek CC M&.(ӷ +60 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +40 h (ӷ M&w& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' ."WCR"\CC.seek :closed C'd(W +416 h (W ."WCR"Hand.open M&U1 +13 h 'R ."MFBPCR"\ATV.dial :air2 F*"  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *j' +115 h 1 M&R% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)P+ +6 h + S)V% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C',  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X+ +6 h + S)3% h % C'& h &W E ;3\\0\\0 ."WCR"\CTV.dial :intake ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)Z  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +207 C'r% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2509h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*X  +65 h  ."MFBPCR"\ARV.dial :carrier F*(  +10 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*`'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\\ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*s  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*f  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*S' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*:  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*N'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*A'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*x' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*t  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254718 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'<  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'F @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'G' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'z @ +75 h @ +50 S)}% h % ."WCR"\CBV.dial between CSV and waste2b C'C  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'm ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'o  ."WCR"\CSV.dial CTV C'  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '%'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'~  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'D' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C''% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'2' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)L% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C'e ` +57 h ` ."WCR"\CS.seek 7.288ml C'g' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'6  '?  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '9'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'l  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'M' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'8  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'{% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'u @ +76 h @ +50 S)O% h % ."WCR"\CBV.dial between CSV and waste2b C'E  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'1 ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'n  'K  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '-'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'v  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'q  +151 h C'a& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&U% h %\  A(R% h %X A(". h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ( ( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (!& h & M&V& h &ӷ M&k% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&,& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(P& h & ."WCR"\SE.seek 18.71 Pucks below Top Plate A(/' +77 h ' M&& h & A([& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &0'Y +25 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(=- M&$&.,(\ #b h -+ ."WCR"\SE.seek 19.04 Pucks below Top Plate A(5' +27 h ' M&*% h %Y A(j& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&I& h & A($& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &#' +22 h ' ."WCR"\SE.seek 20.04 Pucks below Top Plate A(' +36 h ' M&p& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +90 h '\  ."WCR"\Elbow.seek CC M&r(ӷ +142 h (ӷ M&Z& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ ."WCR"\Forearm.seek CC M&X' +91 h ' ."WCR"Hand.close M&(/ +150 h / ."WCR"\CC.seek :open C'W( +416 h ( A(& h & A(`& h & M&\\& h &ӷ M&T% h % ."WCR"\Forearm.seek :retract M&B'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&b& h & A(h& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &_'Y +93 h 'Y ."WCR"\Elbow.hold M&s, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&\ % h %Y A(f& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&7'υ +21 h 'υ M&& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &H' +22 h ' A(S% h % ."downElevator"\SE.seek :down A(:'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (4% h %X M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&N1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&A(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&&/ +150 h / M&x& h &- M&^% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +91 h ' .t\CC.seek :closed C'}(W +416 h (W C'C& h &W .tHand.open M&1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'o% h % .t\CSV.dial CSR C'  +38 h  C'~% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'%'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'2& h &W C' % h %4 .t\CSV.dial :flush C'L  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'e% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'6  .t\CTV.dial CSV C'?  +38 h  +113  .t\CBV.dial :waste1 C'g @ +75 h @ .t\CS.seek 0ml C'l'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'9& h &W C'8% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'{'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +37 h @ C'% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'O  +38 h  .t\CBV.dial :waste2b C'E  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'1& h &W C'% h %4 .t\CSV.dial :flush C'n  +76 h  .t\CS.seek 5ml C'K'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'v% h %p .t\CS.seek 7ml C'-'i +415 h 'i ."connect"\CSV.dial CBV C'q .t\CBV.dial CSV C'a  +38 h +75 .t\CTV.dial :air C'U  +151 h .t\CS.seek 0ml C'R'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'"'0 +506 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'V'4 +507 h '4 C'k& h &W C',% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'P'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +38 h @ C'% h %0 .t\CS.seek 5ml C'['p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'='4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'j +76 h .t\CS.seek 0ml C'I'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'$& h &W C'% h %4 .t\CSV.dial CSR C'p  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'J'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'/ +113 h .t\CTV.dial :intake C'0  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'Z' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'>  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'5  +113 h  C'#% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'3  'd  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'`'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'\\  +38 h  C'T% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  +38 h  .t\CBV.dial :waste2b C'r  +75 h  .t\CS.seek 0ml C'i'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'\  +38 h  C'f% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C'S .t\CBV.dial CSV C'B  +38 h +75 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'4'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'N'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'_  +38 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C't'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C': +113 h .t\CTV.dial :air .\CS.seek 0ml C'H'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C's +38 h .t\CS.seek 0ml C'&'4 +506 h '4 C'x& h &W C'^% h %4 .t\CSV.dial :flush C'Y  +38 h  .t\CS.seek 3ml C' '0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'A'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C')'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +507 h '0 .t\CSV.dial :waste C'C +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'm& h &W C'% h %4 .t\CSV.dial :flush C'o  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +37 h @ C'z% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '%  +38 h  +113  .t\CBV.dial :waste1 C'' @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'2& h &W C' % h %4 .t\CSV.dial :flush C'L  +76 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'e% h %p .t\CS.seek 7ml C'y'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'6  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'g'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'9% h %4 .t\CSV.dial CSR C'8  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'l'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'M' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'{  +37 h +76 .t\CTV.dial :intake C'O  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'E' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'u  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'v +113 h .t\CS.seek 0ml C'K'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&a& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +7 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'"( +416 h ( M&k& h &ӷ M&,% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +264 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &P'η +91 h 'η .tHand.open M&[1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&$1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &p(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.seek :garage M&J'η +91 h 'η .tHand.close M&// +150 h / M&0& h &- M& % h %η .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +264 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'3& h &W .tHand.open M&d1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'\\& h &W C'T% h %4 .t\CSV.dial CSR C'`  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'X'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'h'[ +462 h '[ .t\CSV.dial :waste C'\ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'f'4 +2010 h '4 #t\Purging external Intake C'i& h &W C'% h %4 .t\CSV.dial :flush C'B  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'4'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'_'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 +113 h .t\CTV.dial :air .\CS.seek 0ml C':'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'H +38 h .t\CS.seek 0ml C's'4 +506 h '4 C' & h &W C'x% h %4 .t\CSV.dial :flush C'^  +38 h  .t\CS.seek 3ml C'Y'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +38 h @ C'F% h %0 .t\CS.seek 5ml C'|'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +507 h '0 .t\CSV.dial :waste C') +75 h .t\CS.seek 0ml C'C'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'z( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &'(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&L1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:32 #\Preparing to sleep until Thu Sep 27 06:01:32 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST27-Sep-18 #\Continuing at 06:01CEST27-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:30:34CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST27-Sep-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2509h25ml.pcr F*e% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*6  +21 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*E  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*! % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*[% h %m ."MFBPCR"\AS.seek 0.82ml F*='q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2511h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ARV.dial :carrier F*5  +10 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*p'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *`'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*b  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*K' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*r  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*\ ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*h  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*B  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*4  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*N  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*_'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2511h25ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*t  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*&% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*A% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +64 h  F*~% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Z'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*2  +32 h  ."MFBPCR"\ATV.dial :air3 F*m % +86 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2513h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +21 h ."MFBPCR"delay 2 seconds +200 F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *}'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*k I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*. n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.53ml F*q' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*[  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*=  +108 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*P  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*J% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2513h25ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*>  +21 h ."MFBPCR"\ATV.dial :air3 F*3 % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*h'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*i  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\  +65 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *f'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*4 % +87 h % F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*7% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*_'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*H  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2516h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*W  +10 h  F*Z% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*% n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*e I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*? % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*O' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*{  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*E% h % \\ ."MFBPCR"\AS.seek 0.326ml F*M'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2516h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*v % +87 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*U% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +21 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*[  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *='n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*/  +31 h  ."MFBPCR"\ATV.dial :air3 F*I % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*0% h % ."MFBPCR"\AS.seek 0.81ml F*#'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +21 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *T'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2518h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*B  +42 h  ."MFBPCR"\ARV.dial :carrier F*h  +11 h  F*f% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +22 h ."MFBPCR"delay 2 seconds +200 F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*c  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*t  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*_'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +22 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*& n +21 h n ."MFBPCR"delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*~'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2518h25ml.pcr F*o% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*L% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*l % +86 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *u' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*M'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2520h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ARV.dial :carrier F*R  +11 h  F*=% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*/  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*I  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*J I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*j % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*#' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*>  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*5'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*d n +22 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*`  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*X' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*K  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*i% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*f % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2520h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*\ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :ASV F*4 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*N  +43 h  F* % h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*7 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *c'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*:'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*H' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*_% h % ."MFBPCR"\AS.seek 0.71ml F*&' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h % H ."MFBPCR"\AS.seek 0.735ml F*|' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*Y% h % Ű ."MFBPCR"\AS.seek 0.72ml F*A' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*)' +7161 h ' ."MFBPCR"\ATV.dial :waste F*W n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*o % +43 h % F*~% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*(' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %  ."MFBPCR"\AS.seek 0.604ml F*2'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Q  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*6% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +21 h % ."MFBPCR"delay 3 seconds +300 F*8% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +21 h F*{% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*E n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*O  +21 h  ."MFBPCR"\AS.seek 0ml F*u'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*n  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*v n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *M'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +202 h 'R ."MFBPCR"\SPE.coast F*<  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*! n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*k  +42 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *-' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*. I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*[  +43 h  F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*=  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b **'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*/ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *0': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*P  +64 h  F*j% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *p'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*V n +65 h n F*>% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *3' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*# I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *5': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*d n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *\\'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*T n +151 h n ."MFBPCR"\AS.seek 0ml F*`'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*b  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*K% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*X% h % ."MFBPCR"\AS.seek 0.96ml F*h'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*B' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*S'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*4 I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *7'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *:' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F*x n +129 h n ."MFBPCR"\AS.seek 0ml F*_'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*H% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*s  +21 h ."MFBPCR"\ATV.dial :air3 F*& % +43 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*~% h %n ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V @CEST1537912815.32 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 1ml F*w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *M'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*a % +108 h % ."MFBPCR"\AS.seek 0ml F*!'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538020892.66 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )k h  C' 'G h  P% %U h  F* *. h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *-  h  .t\SPE.configure SPEconfig F*\ 2 *,% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)[% h % .t\SS.configure SSconfig C'q& S)#.0X h  S)p  +37 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %#' +21 h '\  ."closeHand"Hand.close .t\Elbow.jog 1500 M&3/ &* +17 h ."readyCS"\CS.reconfigure CSfast .\CS.home.seek 0ml ."rdySampler"\SS.reconfigure SSconfig C'#d @b '"'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:31:31CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #8) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&B1 +150 h 1 M&4& h &_ M&S% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &N(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &7'η +91 h 'η .tHand.close M&/ +150 h / M&c& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &x' +90 h ' .t\CC.seek :closed C'H(W +416 h (W C' & h &W .tHand.open M&_1 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'F& h &W C'^% h %4 .t\CSV.dial CSR C'|  +151 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'Y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'A% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'W'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'o'4 +2010 h '4 #t\Purging external Intake C'~& h &W C')% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z  ' h +76 .t\CTV.dial :intake C'm  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'L'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'(  +76 h #t\Flushing upper manifold C'%& h &W C' % h %4 .t\CSV.dial :flush C'2  +75 h  .t\CS.seek 5ml C'Q'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +506 h '0 .t\CSV.dial :waste C'D +38 h .t\CS.seek 0ml C'6'4 +506 h '4 C'y& h &W C'g% h %4 .t\CSV.dial :flush C'8  +38 h  .t\CS.seek 3ml C'9'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C'% h %0 .t\CS.seek 5ml C'{'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +507 h '0 .t\CSV.dial :waste C'O +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&u& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +6 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'<( +416 h ( M&w& h &ӷ M&a% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &;(- +265 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&+1 +150 h 1 M&@% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:31:36CEST30-Aug-18\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:31:31CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*]% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'\ & ."readyMFB"\AFV.select :holdingCoil h & F* M&k% h %\  A(G% h %X A(U. h ., A(.& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (-& h &  M&,& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &q( +6 F*}% h %&K ."readyMFB"\ATV.dial :waste2 F*= % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +159 h ( M&R& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(*& h &  .t\SE.seek -0.29 Pucks below Top Plate A(/'\\ +264 h '\\ A(& h &  M&I& h & .t\Elbow.seek 2 M&$( +7 h ( M&J& h & A(j& h &  .t\Forearm.seek 2 + 300 counts M& '; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down M&P& h & A(>'X M&p% h %; .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.seek CC M&T(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'f(W +416 h (W .tHand.open M&1 +150 h 1 M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)`% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % .t\CS.seek 6ml C'<' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C' +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b '0' +710 h ' .t\CSV.dial CSR C'`  +150 h  C'% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'r' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 'b% h %  .t\CS.seek 4.5ml C'd'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .t\CS.seek 1.275ml C'4' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'S   '#9 h 9d +100 C' 9 h 9d +100 C'9 h 91d +100 C'B9 h 9J d +100 C'9 h 9bd +100 C'c9 h 9zd +100 C'9 h 9d +100 C'X9 h 9d +100 C'9 h 9 d +100 C'9 h 9$d +100 C':9 h 9(d +100 C'N9 h 9 ,d +100 C'K9 h 9$0d +100 C'9 h 9<4d +100 C' 9 h 9S8d +100 C'_9 h 9kd +100 C' 9 h 9Td +100 C'm9 h 9kd +100 C'29 h 9d +100 C'L9 h 9d +100 C'9 h 9d +100 C'Q9 h 9d +100 C'9 h 9d +100 C'e9 h 9d +100 C'9 h 9 d +100 C'o9 h 9 d +100 C'%9 h 96d +100 C'?9 h 9Ld +100 C'y9 h 9bd +100 C'g9 h 9xd +100 C'9 h 9d +100 C'69 h 9d +100 C'l9 h 9d +100 C'9 h 9d +100 C'89 h 9d +100 C'99 h 9d +100 C'9 h 9d +100 C'O9 h 9(d +100 C'u9 h 9>d +100 C'{9 h 9Td +100 C'9 h 9id +100 C'D9 h 9d +100 C'n9 h 9d +100 C'E9 h 9d +100 C'9 h 9d +100 C'&9 h 9d +100 C'H9 h 9d +100 C'a9 h 9  d +100 C'v9 h 9 d +100 C't9 h 9 *d +100 C''9 h 9 ?d +100 C'C9 h 9 Td +100 C'+9 h 9 j d +100 C'w9 h 9 $d +100 C'19 h 9 (d +100 C'M9 h 9 ,d +100 C']9 h 9 0d +100 C'k9 h 9 4d +100 C'G9 h 9 8d +100 C'U9 h 9 9 h 9 Xd +100 C'39 h 9 kd +100 C'9 h 9 ~d +100 C'<9 h 9 d +100 C'`9 h 9 d +100 C'9 h 9 d .t\CH.hold 5:00,85C C'0   P h  +30000  C'b9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'r'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'd @ +38 h @ .t\CS.seek 5ml C'#'p +1372 h 'p .t\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'B  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'c'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'X  +19 h C'% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C':  +38 h C'N% h %p .t\CS.seek 7ml C'K'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +37 h @ .t\CS.seek 10ml C' ' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'_ ` +132 h ` #tLysing Complete C's& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&F% h %\  A(% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (x( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ("& h & M&A& h &ӷ M&^% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &|( +143 h ( M&& h & A(W& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +89 h ' A()& h & .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' M&Y& h & A(Z& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y .t\Elbow.hold M&(, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ .t\SE.seek 20.04 Pucks below Top Plate A(' +27 h ' M& % h %Y A(m& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&2'υ +21 h 'υ M&L& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &Q' +22 h ' .t\SE.seek 21.04 Pucks below Top Plate A(' +36 h ' M&e& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +90 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'6( +416 h ( A(l& h & A(& h & M&8& h &ӷ M&9% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &O( +142 h ( M&u& h & A({& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&D, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &n1 +150 h 1 M&E% h %Y A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&&'υ +21 h 'υ M&H& h & A(a& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &v' +22 h ' A(t% h % ."downElevator"\SE.seek :down A(''X +53 h 'X ."downElevator"\SE.coast A(5 +47 (C% h %X M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +89 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'1 @ +19 h @ .t\CS.seek 10ml C'M' +7 h ' .t\CSV.dial between air and CTV C'] ` +18 h ` .t\AIV.dial :bypass C'k { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'G& h & .tHand.open M&U1 +2 h 1 M&.& h & M&-% h %\  .t\Elbow.seek FlushPuck::Garage M&@(- +166 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&='η +91 h 'η .tHand.close M&\ / +150 h / M&& h &- M&z% h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +265 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'j(W +416 h (W C'!& h &W .tHand.open M&V1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%J +151 h P%P% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%q' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%p' +1597 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%/  C'  h +75 .t\PSV.dial :mixloop P%$ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%>  h .t\PS.seek 0ml P%3' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%<' +2609 h ' .t\PSV.dial :mixloop P%` +38 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%0  +37 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :mixloop P%4 +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&d& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&& h &ӷ M&X% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &c(- +264 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&S% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'_& h & M&s% h %\  A(F% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( (~ +358 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b ("& h &~ M&A& h &- M&^% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&W& h & A()& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &Y'н +89 h 'н A(Z& h &~ .t\SE.seek 20.71 Pucks below Top Plate A(T' +57 h ' A(& h &~ M& & h & .t\Elbow.seek 3 M&m( +7 h ( M&L& h & A(& h &~ .t\Forearm.seek 3 + 300 counts M&e'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&5& A(~'X h & M&|% h %- .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&Q1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'o  +151 h  .t\CSV.dial CTV C'f  +18 h  .t\CS.seek 5ml C'l'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'8'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%9  +38 h  .t\PS.seek 2.5ml P%u' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%n  +151 h  .t\PS.seek :empty P%E' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'H  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'a @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C't  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'O @ +38 h @ .t\CS.seek 10ml C'C' +2647 h ' .t\CSV.dial CTV C'+  +37 h  .t\CS.seek 0ml C'1'4 +2648 h '4 .t\CBV.dial :waste1 C'] @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'k  +151 h C'G& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&U% h %\  A(.% h %X A(-. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (&& M&& h &  ."raiseStack"\SE.seek :upperLimit h &ӷ A(''Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&x' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'D( +23 h 'Z +393 ( A(\ & h &  A(& h &  M&z& h &ӷ M&w% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +142 h ( M&& h & A(@& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &*' +93 h ' .t\Elbow.hold M&6, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &!1 +150 h 1 M&V% h % A(,& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&J'; +20 h '; M&P& h & A(I& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +23 h ' A(q% h %Z ."downElevator"\SE.seek :down A(i'X +100 (p% h % M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%$  +2 A(6 +111 h  P%>% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%3  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%`  +38 h .t\CBV.dial AIV C'<  +37 h .t\PRV.dial between CBV and air P%0  +19 h  .t\PRV.dial :air P%  +19 h  P%4% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %b' +250 h ' .t\PRV.dial :waste2 P%d @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb % ' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&R& h & M&\\% h %\  .t\Elbow.seek FlushPuck::Garage M&j(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&:/ +150 h / M&N& h &- M&r% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &S(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  C'^& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%W @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%)  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%Z  +75 h  .tdelay 2 seconds +200 P%c% h % .t\PS.seek 1.499ml P%' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P% ' +1094 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%K +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%5% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %|'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%Y% h %r .t\PS.seek 0.475ml P%?' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%y  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%e'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%T  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%~  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %m' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%# @ +75 h @ .t\PS.seek 0ml P%Q' +2610 h ' .t\PSV.dial :air P%(  +75 h  .t\PS.seek 2.5ml P%o' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%f +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%2' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%M' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%l +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%9% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'{ & +50 h & .t\AWV.select :waste C' +6 P%g% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %%'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'n { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %u' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%H' +2610 h ' .t\PSV.dial :PML P%a +37 h .t\PS.seek 0ml P%t' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'O& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*8 @@T .t\AIV.dial :bypass F*+  .t\PSV.dial PSR P%C +22 h  ."MFBPCR"delay 20 seconds +91 h P%]% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%k' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*G I +129 h I F*U% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *.'6 +66 .t\PSV.dial :waste1 P%-  +37 h  .t\PS.seek 0ml P%&' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%[' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*\ 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%z' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*w % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%@  +38 h  .t\PS.seek 0ml P%x' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*v  +22 h  F*!% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *V' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%, +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%P' +217 ."MFBPCR"\ATV.dial :AIV F*I I +43 h I F*q% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%; +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%p' +2609 h ' .t\PSV.dial PSR P%/ +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%$' +739 h ' ."MFBPCR"delay 5 seconds +500 F*>9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*i% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%E +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%`' +2610 h ' .t\PSV.dial PSR P%< +113 h P%0% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%4  +37 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%d +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%b' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %R'k +2678 h 'k .t\PSV.dial :waste1 P%\\  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*X I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *6'V +849 h ' .t\PSV.dial :air P%:  +113 h  .t\PS.seek 2.5ml P%N' +2610 h ' .t\PSV.dial :mixloop P%r +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb % ' +5107 h ' .t\PSV.dial :air P%B  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*_  +43 h  F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*D I +42 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %^' +618 h ' ."MFBPCR"\ATV.dial :waste F*W n +129 h n ."MFBPCR"\AS.seek :empty F*s'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*)  +86 h  F*Z% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *c' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *L'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'K% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'5' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'Y @ +37 h @ .t\CS.seek 0ml C'|'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*y  +22 h ."MFBPCR"delay 3 seconds +300 F*?% h %_ ."MFBPCR"\AS.seek 0.434ml F*T'ݏ +110 h '4 .t\CSV.dial CSR C'~  +37 h  C'e% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*#  +22 h F*m% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*('& +324 h '& ."MFBPCR"delay 3 seconds +98 h 'P .tdelay 5 seconds +202 ."MFBPCR"\ATV.dial ASV F*Q n +107 h n F*f% h %& ."MFBPCR"\AS.seek 0.092ml F*o'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'2  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'l'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&9& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +7 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'%( +416 h ( M&& h &ӷ M&u% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &+'η +91 h 'η .tHand.open M&C1 +150 h 1 M&]% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'G& h & M&U% h %\  A(-% h %X A(7. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (k(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&&& h &- M&}% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &[( +166 h ( M&& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +89 h 'н A(& h &~ ."WCR"\SE.seek 19.71 Pucks below Top Plate A(.' +66 h ' A( & h &~ M&w& h & ."WCR"\Elbow.seek 3 M&\ ( +7 h ( M&@& h & A(v& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&!'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &z/ +150 h / ."downElevator"\SE.seek :down A(,'X M&& h & M&I% h %- ."WCR"\Forearm.seek :retract M&q'\ +38 h '&0 ."MFBPCR"delay 3 seconds +28 h 'X ."downElevator"\SE.coast A(5 +25 h '\  ."WCR"\Elbow.seek CC M&x(ӷ +142 h (ӷ M&V& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' ."WCR"\CC.seek :closed C'/(W +14 ."MFBPCR"\ATV.dial :waste2 F** % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +359 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +51 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *i' +18 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +7 h + S)E% h % C'$& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'3  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %0 .t\CS.seek 5ml C'`'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'd +76 h .t\CS.seek 0ml C'i'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'X& h &W C'% h %4 .t\CSV.dial CSR C'r  +151 h  C'_% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'<'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'k' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'4 +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'j' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'B  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'\\  +113 h  C':% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'/  +38 h  +113  .t\CBV.dial :waste1 C'N @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'6  +75 h  .t\CS.seek 0ml C'K'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'1 ')  +38 h +75 .t\CTV.dial :air C'D  +151 h .t\CS.seek 0ml C'~'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'| +37 h .t\CS.seek 0ml C'e'4 +507 h '4 #t\Flushing upper manifold C'm& h &W C'#% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'5% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +112 h .t\CTV.dial :air .\CS.seek 0ml C'?'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'$'0 +507 h '0 .t\CSV.dial :waste C'c +38 h .t\CS.seek 0ml C'2'4 +506 h '4 C'Q& h &W C'% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'f'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'l% h %0 .t\CS.seek 5ml C'L'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'9'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +507 h '0 .t\CSV.dial :waste C'F +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'A& h &W C'u% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +37 h @ C'S% h %p .t\CS.seek 7ml C'%'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  'M  +38 h  +113  .t\CBV.dial :waste1 C'U @ +37 h @ .t\CS.seek 0ml C']'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'7& h &W C'G% h %4 .t\CSV.dial :flush C'-  +76 h  .t\CS.seek 5ml C'O'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +38 h @ C'&% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tDisinfecting external Intake with kill C'& h &W C' % h %4 .t\CSV.dial CSR C'v  +38 h  C't% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'w'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z '@  +37 h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'I' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'[  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '!'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'*% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C';'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'V' +1372 h ' .t\CSV.dial CBV C'. +113 h .t\CS.seek 0ml C','4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +6 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&3/ +150 h / .t\CC.seek :open C''( +416 h ( M&p& h &ӷ M&P% h % .t\Forearm.seek :retract M&0'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &E(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&`1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&X1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &r(- +7 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&4/ +150 h / M&& h &- M&k% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&/1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'W% h %4 .t\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '6% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'K& h &W C' % h %4 .t\CSV.dial :flush C')  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D +75 h .t\CTV.dial :intake C'Y  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'~'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'|  +75 h #t\Flushing upper manifold C'& h &W C'm% h %4 .t\CSV.dial :flush C'#  +76 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +38 h @ C'R% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'('4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'T'0 +507 h '0 .t\CSV.dial :waste C'? +38 h .t\CS.seek 0ml C'c'4 +506 h '4 C'$& h &W C'Q% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'y'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'f'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +507 h '0 .t\CSV.dial :waste C'9 +75 h .t\CS.seek 0ml C'F'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&b& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &A(ӷ +6 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&C/ +150 h / .t\CC.seek :open C'S( +416 h ( M&& h &ӷ M&M% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &U(- +264 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&-1 +150 h 1 M&]% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Completed [mbaPhase] begun 06:01:48 #\Preparing to sleep until Sat Sep 29 06:01:48 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:01CEST29-Sep-18 #\Continuing at 06:01CEST29-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:33:01CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:01CEST29-Sep-18" +235125 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2709h25ml.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*v% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*[  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b **'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*0  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +10 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*N  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*,' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*6  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*D'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +21 h n ."MFBPCR"delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.6ml F*|'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*e  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Z'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*5 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2711h25ml.pcr F*(% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +86 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*L% h %n ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*C% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*7  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*-% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*!% h %V ."MFBPCR"\AS.seek 0.5ml F*;'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*3  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*{  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*p I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*x n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.53ml F*o' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*`  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*X'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.6ml F*i'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*4% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*k  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*E  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2713h25ml.pcr F*r% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*B  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*6'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*5'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*?  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ARV.dial :carrier F*b  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*7  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +22 h ."MFBPCR"delay 2 seconds +200 F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *%'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*a n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*& I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*8' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*v'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*w'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*t  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*I% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2716h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*V  +21 h ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*J% h %Z ."MFBPCR"\AS.seek 0.81ml F*\ '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*>  +65 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*4  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +21 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*)  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*|  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*# n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*5' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*T  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*?  +22 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*c'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*9' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*u% h % \\ ."MFBPCR"\AS.seek 0.326ml F*C'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2718h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *7'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*-% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*%'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +64 h  F*8% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*z  +32 h  ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F**'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*4  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*i  +21 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *='Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*< I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*d % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*B  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*\\'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*'  +22 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*/ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*,  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*6  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Y  +151 h F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *|' +424 h ' ."MFBPCR"\ATV.dial :ASV F*~ n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*#'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *$' +424 h ' ."MFBPCR"\ATV.dial :air3 F*5% h % ."MFBPCR"\AS.seek 0.53ml F*?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.71ml F*2' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*c% h % H ."MFBPCR"\AS.seek 0.735ml F*l' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*y% h % Ű ."MFBPCR"\AS.seek 0.72ml F*L' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*f n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*u% h % zG ."MFBPCR"\AS.seek 0.365ml F*9' +7161 h ' ."MFBPCR"\ATV.dial :waste F*b n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*C% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h % ."MFBPCR"\AS.seek 0.579ml F*"' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %  ."MFBPCR"\AS.seek 0.604ml F*7'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*G% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*& n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*%'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*8 % +108 h % F*]% h %V ."MFBPCR"\AS.seek 0.01ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*v% h % q ."MFBPCR"\AS.seek 0.605ml F*z'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*@% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*t' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*I n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*['V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F**  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *V' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *H'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*; n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +202 h 'R ."MFBPCR"\SPE.coast F*{  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*J'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*3 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*p  +43 h  F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *\ ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*x I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *P'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*`  +43 h  F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*>% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*0% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *_' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*4 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*X  +42 h  F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*=  +64 h  F*d% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *r'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*E n +65 h n F*B% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*: I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*N  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\\': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*/ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*W n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %0 ."MFBPCR"\AS.seek 0.985ml F*)' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*D% h % ."MFBPCR"\AS.seek 0.97ml F*1'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*~ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *m'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *(' +9988 h ' ."MFBPCR"\ATV.dial AIV F*5 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*$' +9880 h ' ."MFBPCR"\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.seek 0ml F*Z'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*?% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*c  +21 h ."MFBPCR"\ATV.dial :air3 F*2 % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*u'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*9'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V @CEST1538085624.45 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *V'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *H'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*q % +108 h % ."MFBPCR"\AS.seek 0ml F*3'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 &6 C'5 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538193708.11 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )p h  C' 'n h  P% %J h  F* *x h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *\  h  .t\SPE.configure SPEconfig F*\ 2 *P% h %V .t\AS.configure ASconfig S)`% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'o& S)#.0X% h % .t\PS.configure PSconfig P%#A d`xb M&% h %\  .t\Forearm.configure LooseForearm M&#Q@2^d^2b && h &- .t\Elbow.configure LooseElbow A(0& h &  .t\SC.configure SCloose M&$ &.,(#b A($.(\ 1~b (% h %X .t\SE.configure SEconfig .\CH.coast C'4  h  A(#QK-0-\ b .t\CH.configure CHconfig .\PH.coast C'\ 2 P%_  h  .t\PH.configure PHconfig P%\ 2 +30 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +7 .tValve.reset P% C' F* +6 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'i% h %p P%X% M&<% h %q ."rdySampler"Intake.select :close h %3 ."rdyProcessing"\PSV.dial :air ."rdySampler"\Exhaust.select :close M&k% ."rdySampler"\CTV.dial :air h %3 P%=  C'd  .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &'\ h ."rdySampler"\CSV.dial :waste1 C'E h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)B h  S)r  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %:' +20 h '\  ."closeHand"Hand.close .t\Elbow.jog 1500 M& / &N* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'''4 S)#.0X 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:33:58CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #9) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&)1 +150 h 1 M&~& h &_ M&1% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &e(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&#/ +150 h / M&& h &- M&R% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +264 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'$& h &W .tHand.open M&Z1 +150 h 1 M&c% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'l  +150 h  C'f% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'L% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'b'[ +463 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'C& h &W C'9% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'j  h +75 .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'-'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'"  +75 h #t\Flushing upper manifold C'a& h &W C'G% h %4 .t\CSV.dial :flush C'7  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'%% h %p .t\CS.seek 7ml C'&'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 +113 h .t\CTV.dial :air .\CS.seek 0ml C']'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'O +37 h .t\CS.seek 0ml C''4 +507 h '4 C'}& h &W C'% h %4 .t\CSV.dial :flush C'v  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'% h %0 .t\CS.seek 5ml C'@'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C't'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'{( +416 h ( M&;& h &ӷ M&q% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &S(- +264 h (- M&U& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &s'η +91 h 'η .tHand.open M&h1 +150 h 1 M&^% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:34:02CEST30-Aug-18\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:33:58CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*F% h %&K #tLoading sh1 puck from tube 3 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&p% h %\  A(n% h %X A(J. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (x(~ +6 F*\ % h %&K ."readyMFB"\ATV.dial :waste2 F*P % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *`' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (o& h &~ M&g& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &>( +166 h ( M&& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &0'н +90 h 'н A(& h &~ .t\SE.seek 18.71 Pucks below Top Plate A(4' +76 h ' A(_& h &~ M&i& h & .t\Elbow.seek 3 M&X( +7 h ( M&<& h & A(k& h &~ .t\Forearm.seek 3 + 300 counts M&d'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down A(B'X M&=& h & M&r% h %- .t\Forearm.seek :retract M& '\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&W(ӷ +143 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +90 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'D& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +151 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S)0 +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'G9 h 9Td +100 C'A9 h 9kd +100 C'79 h 9d +100 C'-9 h 9d +100 C'%9 h 9d +100 C' 9 h 9d +100 C'89 h 9d +100 C'&9 h 9d +100 C']9 h 9 d +100 C'9 h 9 d +100 C'a9 h 96d +100 C'9 h 9Ld +100 C'}9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'w9 h 9d +100 C'9 h 9d +100 C'v9 h 9d +100 C' 9 h 9d +100 C'z9 h 9d +100 C'9 h 9(d +100 C'[9 h 9>d +100 C'@9 h 9Td +100 C'9 h 9id +100 C'O9 h 9d +100 C'*9 h 9d +100 C'I9 h 9d +100 C'V9 h 9d +100 C'29 h 9d +100 C'?9 h 9d +100 C'q9 h 9  d +100 C'U9 h 9 d +100 C'T9 h 9 *d +100 C'.9 h 9 ?d +100 C'+9 h 9 Td +100 C'h9 h 9 j d +100 C';9 h 9 $d +100 C'!9 h 9 (d +100 C'H9 h 9 ,d +100 C'F9 h 9 0d +100 C'p9 h 9 4d +100 C'n9 h 9 8d +100 C'J9 h 9 9 h 9 d +100 C'09 h 9 d +100 C'9 h 9 d +100 C'K9 h 9 d +100 C'N9 h 9 d +100 C'49 h 9 d +100 C'd9 h 9 1d +100 C'B9 h 9 Dd +100 C' 9 h 9 Xd +100 C'X9 h 9 kd +100 C' 9 h 9 ~d +100 C'{9 h 9 d +100 C'9 h 9 d +100 C'D9 h 9 d .t\CH.hold 5:00,85C C'   P h  +30000  C'x9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'6'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'/ @ +38 h @ .t\CS.seek 5ml C':'p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'| @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C')  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'` @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'R +113 h .t\CBV.dial CSV C'  +18 h C'#% h %4 .t\CS.seek 5ml C'5'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'(  +37 h C'e% h %p .t\CS.seek 7ml C','i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'Y @ +38 h @ .t\CS.seek 10ml C'$' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'Z ` +132 h ` #tLysing Complete C'c& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&% h %\  A(m% h %X A(f. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (Q( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ('& h &  M&L& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &l( +143 h ( M&u& h & A(b& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &C' +89 h ' A(9& h &  .t\SE.seek 0.71 Pucks below Top Plate A('K +254 h 'K M&y& h & A(j& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&", +6 h , .t\Elbow.reconfigure TightElbow A(M- M&$&.,(\ #b h -+K .t\SE.seek 1.04 Pucks below Top Plate A(t' +27 h ' M&G% h % A(A& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&7'; +21 h '; M&-& h & A(%& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +22 h ' .t\SE.seek 2.04 Pucks below Top Plate A(8' +36 h ' M&&& h & M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&a(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(w& h &  A(& h &  M&v& h &ӷ M& % h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&[& h & A(@& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&O, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &*1 +150 h 1 M&I% h % A(V& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&2'; +20 h '; M&?& h & A(q& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +23 h ' A(T% h % ."downElevator"\SE.seek :down A(.'X +100 (+% h %B M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'! @ + A(6 +17 h @ .t\CS.seek 10ml C'H' +7 h ' .t\CSV.dial between air and CTV C'F ` +19 h ` .t\AIV.dial :bypass C'p { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&J1 +2 h 1 M&\ & h & M&P% h %\  .t\Elbow.seek FlushPuck::Garage M&^(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&s'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'_& h &W .tHand.open M&=1 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%k +150 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%0  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%K +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%N' +2609 h ' ."connect"\PRV.dial CBV P%4  .t\CBV.dial PRV C'd  h +76 .t\PSV.dial :mixloop P%B +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%X' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%{' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%x' +2609 h ' .t\PSV.dial :mixloop P%~ +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&/& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C':( +416 h ( M&R& h &ӷ M&% h % .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&Y1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'Z& h & M&c% h %\  A(% h %X A(m. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (f(~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b ('& h &~ M&L& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &u( +166 h ( M&b& h & A(9& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &W'н +89 h 'н A(\\& h &~ .t\SE.seek 17.71 Pucks below Top Plate A(y'* +86 h '* A(j& h &~ M&M& h & .t\Elbow.seek 3 M&G( +7 h ( M&A& h & A(-& h &~ .t\Forearm.seek 3 + 300 counts M&%'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &&/ +150 h / ."downElevator"\SE.seek :down A(]'X M&C& h & M&l% h %- .t\Forearm.seek :retract M&'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' .t\CC.seek :closed C't(W +416 h (W .tHand.open M& 1 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'v'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%[' +2609 h ' .t\PSV.dial :mixloop P%@ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%a +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%*  +151 h  .t\PS.seek :empty P%I' +2610 h ' .t\CBV.dial :waste1 C'V @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'?  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'q @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'T  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'z ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'+' +2647 h ' .t\CSV.dial CTV C'h  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'! @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'2  +150 h C'F& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&p% h %\  A(n% h %X A(J. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (\ ( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (P& h &  M&8& h &ӷ M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &g( +143 h ( M&& h & A(.& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Q' +89 h ' A($& h &  .t\SE.seek 1.71 Pucks below Top Plate A(O': +244 h ': M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&;, +6 h , .t\Elbow.reconfigure TightElbow A(s- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(S' +27 h ' M&i% h % A(^& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'; +20 h '; M&& h & A(_& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &=' +23 h ' .t\SE.seek 3.04 Pucks below Top Plate A(o' +36 h ' M&k& h & M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +89 h '\  .t\Elbow.seek CC M&0(ӷ +143 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'd( +416 h ( A(B& h &  A( & h &  M&N& h &ӷ M& % h % .t\Forearm.seek :retract M&X'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&{& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &D' +92 h ' .t\Elbow.hold M&~, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&/% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &|'; +20 h '; M&`& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &E' +22 h ' A(3% h % ."downElevator"\SE.seek :down A(R'X +100 (% h %1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%e  +2 A(6 +111 h  P%6% h % .t\PS.seek 0.499ml P%Y' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%1 +37 h .t\PRV.dial :air P%#  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%Z  +38 h .t\CBV.dial AIV C'c  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%m  +19 h  P%'% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %L' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %5' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'b& h & .tHand.open M&91 +2 h 1 M&\\& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&j(- +166 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.seek :garage M&-'η +91 h 'η .tHand.close M&,/ +150 h / M&&& h &- M&C% h %η .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +264 h (ӷ M&W& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'y(W +416 h (W C'%& h &W .tHand.open M&]1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C':& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%"  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%7' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%f' +1094 h ' .t\PSV.dial :air P%H  +38 h  .t\PS.seek 0ml P%w' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %@'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%a% h %r .t\PS.seek 0.475ml P%*' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%[  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%V'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%?  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%q  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %T' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%z +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%v' +2610 h ' .t\PSV.dial :air P%h  +75 h  .t\PS.seek 2.5ml P%+' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%! +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%2' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%F  +76 h  .t\PS.seek 2.5ml P%p' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%n +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%J  +75 h P%P% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %8' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'U & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %.'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'$ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%s' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'i& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'^& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*_  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%k +22 h  ."MFBPCR"delay 20 seconds +91 h P%r% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%Q' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*g I +129 h I F*O% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +65 .t\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%K' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%S' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%= +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%;' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*4  +22 h ' .t\PSV.dial PSR P%< +20 h  F*t% h %6 ."MFBPCR"\AS.seek 0.329ml F*B'ͯ +93 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%N' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*0  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b */' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%`' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*3% h % ."MFBPCR"\AS.seek 0.469ml F*X' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%) +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%|' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%o  +37 h  .t\PS.seek 0ml P%e' +740 h ' ."MFBPCR"delay 5 seconds +500 F*69 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*E n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*R% h % ."MFBPCR"\AS.seek 0.041ml F*\ '% +337 h ' .t\PSV.dial PSR P%I +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%1' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%# +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Z' +2610 h ' .t\PSV.dial PSR P%c +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%Y' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%L' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%9 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %\\'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %M' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*A I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *~'V +850 h ' .t\PSV.dial :air P%,  +113 h  .t\PS.seek 2.5ml P%&' +2609 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %(' +5107 h ' .t\PSV.dial :air P%-  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %W' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *l'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*d I +43 h I F*%% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +171 h ' .t\PSV.dial :mixloop P%G +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %:' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*}'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*"  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*5  +43 h F*7% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *H'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'f  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'w  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'a @ +38 h @ .t\CS.seek 0ml C'@'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*[  +21 h ."MFBPCR"delay 3 seconds +300 F**% h %_ ."MFBPCR"\AS.seek 0.434ml F*?'ݏ +110 h '4 .t\CSV.dial CSR C'q  +38 h  C'V% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'z'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F*T% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*h'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*v n +108 h n F*!% h %& ."MFBPCR"\AS.seek 0.092ml F*+'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'F' +1625 h ' ."connect"\CSV.dial CTV C'2  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'n'4 +2647 h '4 C'J& h &W #tGaraging FlushPuck in Collection Clamp M&P& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &p(ӷ +6 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&8' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'.( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &_'η +91 h 'η .tHand.open M&k1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'g& h & M&O% h %\  A(>% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Q(~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&K& h &- M&=% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &S( +166 h ( M&4& h & A(t& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &<'н +89 h 'н A( & h &~ ."WCR"\SE.seek 16.71 Pucks below Top Plate A('; +96 h '; A(;& h &~ M& & h & ."WCR"\Elbow.seek 3 M&B( +6 h ( M&& h & A(0& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&x'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &N/ +150 h / ."downElevator"\SE.seek :down A('X M&{& h & M& % h %- ."WCR"\Forearm.seek :retract M&3'\ +9 h '&0 ."MFBPCR"delay 3 seconds +82 h '\  ."WCR"\Elbow.seek CC M&(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&/& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +75 ."MFBPCR"\ATV.dial :waste2 F*) % +16 h ' ."WCR"\CC.seek :closed C'D(W +27 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +389 h (W ."WCR"Hand.open M&o1 +150 h 1 M&|% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +21 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *R' +49 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)X+ +6 h + S)I% h % C'e& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'#  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *K' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*_% h % ."MFBPCR"\AS.seek 0.579ml F*4' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*0 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*s  +43 h  ."MFBPCR"\AS.seek 0ml F*N'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*S % +107 h % F*<% h %V ."MFBPCR"\AS.seek 0.59ml F*/' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +107 h n ."MFBPCR"\ASV.dial :wasteR F*x% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*)  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*3' +625 h ' ."MFBPCR"\ATV.dial :air3 F*. % +21 h % F*+% h % ."MFBPCR"\AS.seek 0.13ml F*o' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +21 h F*E% h % ."MFBPCR"\AS.seek 0.23ml F*I' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +21 h % ."MFBPCR"delay 3 seconds +300 F*#% h % ."MFBPCR"\AS.seek 0.255ml F*`'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*Q% h %X ."MFBPCR"\AS.seek 0.24ml F*X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*c  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *m'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*1 @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*\ I +86 h I ."MFBPCR"\AS.seek 0ml F*9'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*R'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *A'\  +1031 h '\  F*b% h %\  ."MFBPCR"\AS.seek 0.5ml F*,'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*M% h %(A ."MFBPCR"\AS.seek 0.6ml F*C'\  +1031 h '\  F*\\% h %\  ."MFBPCR"\AS.seek 0.5ml F*('(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *j' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*~ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +3472 h 'V ."MFBPCR"\SPE.coast F*-  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2909h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*%  +64 h  ."MFBPCR"\ARV.dial :carrier F*G  +11 h  F*L% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*d  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *l'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*a % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +107 h  ."MFBPCR"delay 7 seconds +700 F*q% h %< ."MFBPCR"\AS.seek 0.594ml F*V'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*[ n +21 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*v' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 F*h  +64 h ."MFBPCR"\ARV.dial :air1 F*!  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*2% h % \\ ."MFBPCR"\AS.seek 0.326ml F*F'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*P % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254561 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'U  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'J @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '$' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'u @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'8' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'i  '  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'p'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'k  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '^' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'r  +38 h  C'O% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'g @ +75 h @ +50 S)>% h % ."WCR"\CBV.dial between CSV and waste2b C'=  +95 h  C'_% h % ."WCR"\CSV.dial between air and CTV C'K ` +56 h ` ."WCR"\CS.seek 7.288ml C't' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '4  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ';'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'0  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C's  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'N' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'S @ +76 h @ +50 S)<% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'x ` +56 h ` ."WCR"\CS.seek 7.288ml C'/' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C')  'B  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '.' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'+  +151 h C'3& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&|% h %\  A(E% h %X A(o. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (e( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (#& h &  M&I& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Q( +143 h ( M&`& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +89 h ' A(X& h &  ."WCR"\SE.seek 2.71 Pucks below Top Plate A('') +235 h ') M&Z& h & A(m& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &\ ' +25 h ' ."WCR"\Elbow.hold M&Y, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(9- M&$&.,(\ #b h -+) ."WCR"\SE.seek 3.04 Pucks below Top Plate A(' +26 h ' M&% h % A(R& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &b'; +21 h '; M&A& h & A(M& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +22 h ' ."WCR"\SE.seek 4.04 Pucks below Top Plate A(\\' +36 h ' M&C& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +89 h '\  ."WCR"\Elbow.seek CC M&~(ӷ +143 h (ӷ M&j& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ ."WCR"\Forearm.seek CC M&%' +91 h ' ."WCR"Hand.close M&G/ +150 h / ."WCR"\CC.seek :open C'L( +416 h ( A(&& h &  A(W& h &  M&]& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&"'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &d( +142 h ( M&5& h & A(7& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&l% h % A( & h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&w'; +21 h '; M&f& h & A(a& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(*'X +100 (q% h %  M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'@& h & .tHand.open M&V1 +2 A(6 h 1 M&T& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&H(- +166 h (- M&?& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.seek :garage M&!'η +91 h 'η .tHand.close M&2/ +150 h / M&v& h &- M&z% h %η .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +265 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'y& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'i% h % .t\CSV.dial CSR C'8  +38 h  C'k% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'p'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'r'[ +463 h '[ .t\CSV.dial :waste C'O +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '^'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'g% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '='p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'K% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  '  +37 h  +113  .t\CBV.dial :waste1 C't @ +75 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C';& h &W C'0% h %4 .t\CSV.dial :flush C's  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'N% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CTV C'<  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'x& h &W C'{% h %4 .t\CSV.dial :flush C')  +76 h  .t\CS.seek 5ml C'B'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'+ .t\CBV.dial CSV C'3  +37 h +76 .t\CTV.dial :air C'|  +150 h .t\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +507 h '0 .t\CSV.dial :waste C'# +37 h .t\CS.seek 0ml C'I'4 +507 h '4 C'& h &W C'`% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'X'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'6% h %0 .t\CS.seek 5ml C'Z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'R'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'A& h &W C'M% h %4 .t\CSV.dial CSR C'C  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'c'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'Q' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +112 h .t\CTV.dial :intake C'\  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'j' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'-  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C',% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'Y'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'G' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'(  'D  +37 h  +113  .t\CBV.dial :waste1 C'& @ +38 h @ .t\CS.seek 0ml C'W'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C']  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '5'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +709 h ' ."connect"\CSV.dial CTV C'%  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'~  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'l  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'f'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'a' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'"  ' +38 h +75 .t\CTV.dial :air C'd  +151 h .t\CS.seek 0ml C'q'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'@ +37 h .t\CS.seek 0ml C'V'4 +507 h '4 #t\Flushing upper manifold C'T& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\\ @ +38 h @ C'?% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C'*'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'e'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'2'4 +506 h '4 C'v& h &W C'z% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.seek 3ml C'!'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'J @ +38 h @ C'n% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'P'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C'i  +37 h  .t\CS.seek 5ml C'8'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +37 h @ C'u% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'p  +38 h  +113  .t\CBV.dial :waste1 C'O @ +38 h @ .t\CS.seek 0ml C'r'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'g% h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C'^'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'K% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C't'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C';% h %4 .t\CSV.dial CSR C'0  +37 h  C's% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C' 'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N '  +37 h +76 .t\CTV.dial :intake C'<  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C' ' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'x'4 +606 h '4 .t\CSV.dial CSR C'{  +113 h  C')% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'/' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'B'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&3& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +6 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&E' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'o( +416 h ( M&& h &ӷ M&`% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &I(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &X'η +91 h 'η .tHand.open M&Z1 +150 h 1 M&m% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'9& h & .tHand.open M&A1 +2 h 1 M&M& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &C(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&'/ +150 h / M&\ & h &- M&Q% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &b' +91 h ' .t\CC.seek :closed C'Y(W +416 h (W C'(& h &W .tHand.open M&D1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C' % h %4 .t\CSV.dial CSR C'W  +113 h  C'5% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'%'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '~% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '7'[ +462 h '[ .t\CSV.dial :waste C'l +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Purging external Intake C'& h &W C'f% h %4 .t\CSV.dial :flush C'"  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'd +75 h .t\CTV.dial :intake C'a  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'q'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'@  +75 h #t\Flushing upper manifold C'& h &W C'T% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'\\% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'h'0 +507 h '0 .t\CSV.dial :waste C'* +38 h .t\CS.seek 0ml C''4 +506 h '4 C'e& h &W C'v% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'['0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'J% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'!'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +507 h '0 .t\CSV.dial :waste C'P +75 h .t\CS.seek 0ml C'y'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&L& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'u( +416 h ( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&>1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:03 #\Preparing to sleep until Mon Oct 01 06:02:03 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST01-Oct-18 #\Continuing at 06:02CEST01-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:35:28CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST01-Oct-18" +235154 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2909h25ml.pcr F*K% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*4  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*0% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*<% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*S  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*{% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*# % +87 h % F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*`'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +21 h F*Z% h %m ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2911h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*o n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*5  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*b% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*~  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*l' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*7  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*f'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*a n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\\% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2911h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*J'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*!'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*i  +64 h  F*k% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast F*#,,'T(20( b ."MFBPCR"\AS.seek 0ml F*'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.01ml F*r' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2913h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*)  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*#  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +21 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F*+' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Z  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +108 h  ."MFBPCR"delay 7 seconds +700 F*9% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*\ ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*,  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2913h25ml.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*-  +22 h ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +21 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*l  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +31 h  ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +21 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F**  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2916h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*k  +42 h  ."MFBPCR"\ARV.dial :carrier F*L  +11 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *.'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*p n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*K I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*4'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*;  +107 h  ."MFBPCR"delay 7 seconds +700 F*^% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*)  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2916h25ml.pcr F*{% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*/  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*E'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*6  +65 h  F*+% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*Q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *I' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep2918h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*7  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*f'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*@  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +22 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*w% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*1  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*?' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*h  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +22 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*n  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*P' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*H  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*!  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*k'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218sep2918h25ml.pcr F*i% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*8  +22 h ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*F% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*=  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*N  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h % ."MFBPCR"\AS.seek 0.81ml F*)'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *6'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep2920h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ARV.dial :carrier F*E  +10 h  F*m% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*c I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*,' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*-  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*o  +22 h  ."MFBPCR"delay 7 seconds +700 F*&% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +22 h n ."MFBPCR"delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*W  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*B  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*~  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep2920h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*l @@T ."MFBPCR"\ASV.dial :SPEdelivery F*f  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*a  +151 h F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *@' +423 h ' ."MFBPCR"\ATV.dial :ASV F*q n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*V  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*w n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *1'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*\\ % +43 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *e' +423 h ' ."MFBPCR"\ATV.dial :air3 F*?% h % ."MFBPCR"\AS.seek 0.53ml F**' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*2' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*n' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*[% h % Ű ."MFBPCR"\AS.seek 0.72ml F*H' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*! n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*P' +7161 h ' ."MFBPCR"\ATV.dial :waste F*L n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*i % +43 h % F*k% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*G' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*g% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +86 h n ."MFBPCR"\ASV.dial :wasteR F*F  +22 h  ."MFBPCR"\AS.seek 0ml F*.'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *='V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*r% h %V ."MFBPCR"\AS.seek 0.01ml F*;' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*4% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*0% h % q ."MFBPCR"\AS.seek 0.605ml F*N'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*s' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*<  +21 h  ."MFBPCR"\AS.seek 0ml F*S'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *{'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*)  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b */' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +202 h 'R ."MFBPCR"\SPE.coast F*U  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*|'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*# n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Z  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *E': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*m  +64 h  F*6% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *9'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*M n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*A  +43 h  F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*X  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *C'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*I n +65 h n F*-% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *(' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*, I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*&  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*D n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *]'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*W'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*5  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*b % +43 h % F*B% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h % ."MFBPCR"\AS.seek 0.96ml F*7'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %0 ."MFBPCR"\AS.seek 0.985ml F*"' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*d% h % ."MFBPCR"\AS.seek 0.97ml F*'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*q I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *w'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *T'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*1  +43 h  F*\\% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*? I +43 h I ."MFBPCR"\AS.seek 0.02ml F*e' +9880 h ' ."MFBPCR"\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F**% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +43 h % F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*P'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*k% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*8% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*='> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*;'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*0% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' @CEST1538258419.86 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*U n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*3 % +108 h % ."MFBPCR"\AS.seek 0ml F*#'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538366523.55 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' '$ h  P% %| h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *`% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)Z% h % .t\SS.configure SSconfig C'+& S)#.0X 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:36:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #10) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'd& h & .tHand.open M&"1 +150 h 1 M&q& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &V(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &w'η +91 h 'η .tHand.close M&/ +150 h / M&1& h &- M&\\% h %η .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +264 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'*(W +416 h (W C'e& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'J& h &W C'z% h %4 .t\CSV.dial CSR C'n  +151 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '['p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'H% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'L'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +2010 h '4 #t\Purging external Intake C'k& h &W C'P% h %4 .t\CSV.dial :flush C'Y  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 'j  h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'>'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'G  +75 h #t\Flushing upper manifold C'p& h &W C'g% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'.% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'r'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'^ +38 h .t\CS.seek 0ml C'4'4 +506 h '4 C'=& h &W C't% h %4 .t\CSV.dial :flush C'0  +38 h  .t\CS.seek 3ml C';'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'N'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'< +75 h .t\CS.seek 0ml C's'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +6 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'U( +416 h ( M&& h &ӷ M&3% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &#'η +91 h 'η .tHand.open M&1 +150 h 1 M&u% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:36:32CEST30-Aug-18\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:36:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*_% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'l& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A($% h %X A(|. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +7 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*` % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *Z' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (+& h &~ M&& h &- M&m% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &6( +166 h ( M&E& h & A(M& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +89 h 'н A(9& h &~ .t\SE.seek 15.71 Pucks below Top Plate A(''L +107 h 'L A(& h &~ M&R& h & .t\Elbow.seek 3 M&A( +6 h ( M&c& h & A(Q& h &~ .t\Forearm.seek 3 + 300 counts M&'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &I/ +150 h / ."downElevator"\SE.seek :down M&X& A(-'X h & M&C% h %- .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&f1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)b+ +6 h + S)W% h % C'd& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  .t\SS.seek 0ml S)' +6 h ' .t\SSV.dial CBV,avoiding: :exhaust S).  +144 h  +7  S)F% h % .t\CBV.dial between SSV and waste2 C'  +19 h  C'K% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'r  +37 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '^'4 +2610 h '4 C' % h %4 S)=% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C't @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'g9 h 9Td +100 C'9 h 9kd +100 C'9 h 9d +100 C'>9 h 9d +100 C'.9 h 9d +100 C'F9 h 9d +100 C'9 h 9d +100 C'K9 h 9d +100 C'r9 h 9 d +100 C'i9 h 9 d +100 C'p9 h 96d +100 C' 9 h 9Ld +100 C'=9 h 9bd +100 C't9 h 9xd +100 C'9 h 9d +100 C'49 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'09 h 9d +100 C';9 h 9d +100 C'N9 h 9d +100 C'<9 h 9(d +100 C'S9 h 9>d +100 C'9 h 9Td +100 C'{9 h 9id +100 C'^9 h 9d +100 C')9 h 9d +100 C' 9 h 9d +100 C'/9 h 9d +100 C'29 h 9d +100 C'*9 h 9d +100 C'39 h 9  d +100 C':9 h 9 d +100 C'h9 h 9 *d +100 C'9 h 9 ?d +100 C'}9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'x9 h 9 (d +100 C'9 h 9 ,d +100 C'_9 h 9 0d +100 C'9 h 9 4d +100 C'$9 h 9 8d +100 C'|9 h 9 & h & A(.& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &F' +22 h ' .t\SE.seek 5.04 Pucks below Top Plate A(' +36 h ' M&K& h & M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +90 h '\  .t\Elbow.seek CC M&p(ӷ +142 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'4( +416 h ( A( & h &  A(& h &  M&0& h &ӷ M&;% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &<( +143 h ( M&S& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &{' +92 h ' .t\Elbow.hold M&^, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M& % h % A(/& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &2'; +21 h '; M&*& h & A(3& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &:' +22 h ' A(h% h % ."downElevator"\SE.seek :down A('X +100 (}% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'x @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C'_ ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'$& h & .tHand.open M&|1 +2 h 1 M&& h & M&`% h %\  .t\Elbow.seek FlushPuck::Garage M&u(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.seek :garage M&#'η +91 h 'η .tHand.close M&l/ +150 h / M&E& h &- M&M% h %η .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'y(W +416 h (W C'& h &W .tHand.open M&X1 +150 h 1 M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%Q +151 h P%C% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%6' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%(' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C''  h +75 .t\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%&  h .t\PS.seek 0ml P%-' +2610 h ' .t\PSV.dial :air P%f  +38 h  .t\PS.seek 2.5ml P%U' +2609 h ' .t\PSV.dial :mixloop P%W +38 h .t\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial :air P%\  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%b' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.seek CC M&Z' +91 h ' .tHand.close M&"/ +150 h / .t\CC.seek :open C',( +416 h ( M&\\& h &ӷ M&%% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &?'η +91 h 'η .tHand.open M&a1 +150 h 1 M&q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&% h %\  A(J% h %X A(T. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (!(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (o& h &~ M&H& h &- M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&L& h & A(P& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & 'н +90 h 'н A(& h &~ .t\SE.seek 14.71 Pucks below Top Plate A(['] +116 h '] A(j& h &~ M&& h & .t\Elbow.seek 3 M&g( +6 h ( M&& h & A(>& h &~ .t\Forearm.seek 3 + 300 counts M&.'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &K/ +150 h / ."downElevator"\SE.seek :down A(r'X M&k& h & M&n% h %- .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.seek CC M&8(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&F1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'i  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'0'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%S' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%t  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%p +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%)  +150 h  .t\PS.seek :empty P% ' +2610 h ' .t\CBV.dial :waste1 C'/ @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'*  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'3 @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'h  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'N ` +18 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'}' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'x'4 +2647 h '4 .t\CBV.dial :waste1 C'_ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'$& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&{% h %\  A(|% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (`( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (2& h &  M&& h &ӷ M&:% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&m& h & A(v& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(e& h &  .t\SE.seek 4.71 Pucks below Top Plate A(^' +214 h ' M&l& h & A(E& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &M' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(#- M&$&.,(\ #b h -+ .t\SE.seek 5.04 Pucks below Top Plate A(O' +27 h ' M&R% h % A(u& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &9'; +21 h '; M&4& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(+' +36 h ' M&Q& h & M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C''( +416 h ( A(A& h &  A(&& h &  M&(& h &ӷ M&f% h % .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &W( +143 h ( M&U& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &d' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &D1 +150 h 1 M&% h % A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&@'; +21 h '; M&"& h & A(5& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &I' +22 h ' A(y% h % ."downElevator"\SE.seek :down A(\\'X +100 (%% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%V  +2 A(6 +111 h  P%~% h % .t\PS.seek 0.499ml P%a' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%q +37 h .t\PRV.dial :air P%B  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%J  +19 h  .t\PRV.dial :air P%T  +19 h  P%o% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %H' +250 h ' .t\PRV.dial :waste2 P%z @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %?' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&P1 +2 h 1 M&& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&j(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&>'η +91 h 'η .tHand.close M&/ +150 h / M&K& h &- M&k% h %η .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'.& h &W .tHand.open M&g1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  C',& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%F @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%G  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%i  +75 h  .tdelay 2 seconds +200 P% % h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%!' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P% ' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%;  +38 h  .tdelay 2 seconds +200 P%b% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%t  +75 h .tdelay 2 seconds +200 P%p% h %r .t\PS.seek 0.475ml P%)' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%S  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%/'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%*  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%3  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %h' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%N +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%}' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%_ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%$  +75 h  .t\PS.seek 2.5ml P%{' +2609 h ' .tdelay 3 seconds +299 .\PSV.dial :PML P%| +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%2% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C': & +50 h & .t\AWV.select :waste C' +6 P%m% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %v'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'e { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%l' +2609 h ' .t\PSV.dial :PML P%E +38 h .t\PS.seek 0ml P%#' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'R& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'u& h &W F*4 @@T .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass .\PSV.dial PSR F*  P%Q +22 h  ."MFBPCR"delay 20 seconds +91 h P%C% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*^% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *M'6 +65 .t\PSV.dial :waste1 P%6  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%9 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%X +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%c +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*A'ͯ +92 h P%&% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%(' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*f % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\  +38 h  .t\PS.seek 0ml P%]' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*D% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%"' +217 ."MFBPCR"\ATV.dial :AIV F*5 I +43 h I F*y% h % ."MFBPCR"\AS.seek 0.469ml F*-' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%W +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%%' +2609 h ' .t\PSV.dial PSR P%Z +113 h P%d% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%@' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%+  +37 h  .t\PS.seek 0ml P%V' +740 h ' ."MFBPCR"delay 5 seconds +500 F*~9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*I n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*\\% h % ."MFBPCR"\AS.seek 0.041ml F*`'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%q' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%B +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%J% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%T' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%o  +38 h  .t\PS.seek 0ml P%a' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%H' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%1  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%K' +2610 h ' .t\PSV.dial :mixloop P%k +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%>  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *n'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*' I +43 h I F*.% h %Q ."MFBPCR"\AS.seek 0.01ml F*g' +171 h ' .t\PSV.dial :mixloop P%r +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %,' +618 h ' ."MFBPCR"\ATV.dial :waste F*F n +129 h n ."MFBPCR"\AS.seek :empty F*='R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*G  +86 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*?  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'!  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C';'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'b' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  't  +38 h  +112  .t\CBV.dial :waste1 C'p @ +38 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*S  +21 h ."MFBPCR"delay 3 seconds +300 F*)% h %_ ."MFBPCR"\AS.seek 0.434ml F**'ݏ +110 h '4 .t\CSV.dial CSR C'3  +38 h  C'/% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'N'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*h% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*0 n +108 h n F*_% h %& ."MFBPCR"\AS.seek 0.092ml F*}'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'$' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'|'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&2& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +6 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&e/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&8% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &l(- +264 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&C% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'& h & M&^% h %\  A(6% h %X A(w. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (9& h &~ M&& h &- M&X% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &O( +166 h ( M&& h & A(s& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &c'н +89 h 'н A(&& h &~ ."WCR"\SE.seek 13.71 Pucks below Top Plate A(M'n +125 h 'n A(& h &~ M&f& h & ."WCR"\Elbow.seek 3 M&A( +7 h ( M&\ & h & A(& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&D'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &(/ +129 h '&0 ."MFBPCR"delay 3 seconds +21 h / ."downElevator"\SE.seek :down M&U& h & A('X M&5% h %- ."WCR"\Forearm.seek :retract M&y'\ +91 h '\  ."WCR"\Elbow.seek CC M&](ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +46 ."MFBPCR"\ATV.dial :waste2 F*Z % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +2 h ' ."WCR"\CC.seek :closed C'%(W +416 h (W ."WCR"Hand.open M&+1 +142 h 'R ."MFBPCR"\ATV.dial :air2 F*@  +8 h 1 M&~% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +13 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *\\' +78 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)-+ +7 h + S) % h % C'V& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'B  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X+ +7 h + S)% h % C' & h &W E ;3\\0\\0 ."WCR"\CTV.dial :intake ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)j  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +207 C'% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0109h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*.  +64 h  ."MFBPCR"\ARV.dial :carrier F*r  +11 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*g n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *n'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*!' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*;  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*x' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*p % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*t'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +107 h  ."MFBPCR"delay 7 seconds +700 F*3% h %< ."MFBPCR"\AS.seek 0.594ml F*/'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*S n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F**  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*_  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254590 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C':  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)m% h % ."WCR"\CBV.dial between CSV and waste2b C'[  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'Y ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'R  '8  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '{'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'Q  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'E  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'C  +38 h  C'^% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'w' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)6% h % ."WCR"\CBV.dial between CSV and waste2b C'X  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C's' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'&  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'f  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'l' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'#  +38 h  C'\ % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'(' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'U' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'O @ +75 h @ +50 S)c% h % ."WCR"\CBV.dial between CSV and waste2b C'5  +95 h  C'W% h % ."WCR"\CSV.dial between air and CTV C'D ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'Z  ."WCR"\CSV.dial CTV C'A  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'M  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '}' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'v  +151 h C'y& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&~% h %\  A(@% h %X A(+. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (V( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (B& h &  M& & h &ӷ M&d% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&"& h & A(J& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(-& h &  ."WCR"\SE.seek 5.71 Pucks below Top Plate A(o' +205 h ' M&& h & A(T& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &`' +25 h ' ."WCR"\Elbow.hold M&a, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(P- h -+ M&$&.,(\ #b ."WCR"\SE.seek 6.04 Pucks below Top Plate A(1' +27 h ' M&z% h % A(\\& h &  ."WCR"\Forearm.force -20 .\Forearm.seek 2 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &L'; +21 h '; M&& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm M&#Q@2^d^\ b ."WCR"\Forearm.seek 2 + 700 counts M&' +22 h ' ."WCR"\SE.seek 7.04 Pucks below Top Plate A(' +36 h ' M&k& h & M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&j& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ ."WCR"\Forearm.seek CC M&.' +91 h ' ."WCR"Hand.close M&r/ +150 h / ."WCR"\CC.seek :open C'H( +416 h ( A(K& h &  A( & h &  M&g& h &ӷ M&F% h % ."WCR"\Forearm.seek :retract M&G'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &'( +142 h ( M&?& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &i' +93 h ' ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&n% h % A(;& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M& '; +21 h '; M&!& h & A(p& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &b' +22 h ' A(x% h % ."downElevator"\SE.seek :down A()'X +100 (3% h % M&t% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&/1 + A(6 + h 1 M&h& h & M&<% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&/ +150 h / M&0& h &- M&N% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C'[& h &W .tHand.open M&1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'8& h &W C'R% h % .t\CSV.dial CSR C'  +37 h  C'Q% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'{'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'E% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'C'[ +463 h '[ .t\CSV.dial :waste C'^ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'u'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'w& h &W C'% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'X'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '&  +38 h  +112  .t\CBV.dial :waste1 C's @ +76 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'% h %4 .t\CSV.dial :flush C'#  +75 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'(% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  +38 h  .t\CBV.dial :waste2b C'5  +75 h  .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing upper manifold C'D& h &W C'U% h %4 .t\CSV.dial :flush C'A  +75 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'M% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'v .t\CBV.dial CSV C'y  +38 h +75 .t\CTV.dial :air C'~  +150 h .t\CS.seek 0ml C'@'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'B +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'd& h &W C'"% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 3ml C'-'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'% h %0 .t\CS.seek 5ml C'T'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'I'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C'z +75 h .t\CS.seek 0ml C'\\'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'% h %4 .t\CSV.dial CSR C'k  +150 h  C' % h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'o .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'`  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'j' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'>  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'1  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'a'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'%  +38 h  +112  .t\CBV.dial :waste1 C'K @ +38 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'g  +37 h  C'F% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '?'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'n  +38 h  C';% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'p' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G  'x +38 h +75 .t\CTV.dial :air C''  +151 h .t\CS.seek 0ml C'3'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'/'4 +506 h '4 #t\Flushing upper manifold C'h& h &W C'<% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'*% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'b .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C')'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +507 h '4 C'0& h &W C'N% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 3ml C'_'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'|% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'[ +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'Y& h &W C'8% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +38 h @ C'% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  'E  +37 h  +113  .t\CBV.dial :waste1 C'^ @ +38 h @ .t\CS.seek 0ml C'C'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'w& h &W C'% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'u'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'&  +75 h  .t\CS.seek 0ml C's'4 +766 h '4 #tDisinfecting external Intake with kill C'9& h &W C'% h %4 .t\CSV.dial CSR C'  +38 h  C'#% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'f'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +416 h ' ."connect"\CSV.dial CBV C'( .t\CBV.dial CSV C'\  +38 h +75 .t\CTV.dial :intake C'c  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'5' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'O  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +607 h '4 .t\CSV.dial CSR C'U  +113 h  C'A% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'M +113 h .t\CS.seek 0ml C'Z'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&@' +90 h ' .tHand.close M&B/ +150 h / .t\CC.seek :open C'+( +416 h ( M&d& h &ӷ M&"% h % .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &-'η +91 h 'η .tHand.open M&T1 +150 h 1 M&I% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'P& h & .tHand.open M&1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &k(- +6 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&o/ +150 h / M&`& h &- M&% h %η .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C'a(W +416 h (W C'& h &W .tHand.open M&%1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'F% h %4 .t\CSV.dial CSR C'  +113 h  C'?% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'.'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'n +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ';'4 +2010 h '4 #t\Purging external Intake C'& h &W C'!% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +76 h .t\CTV.dial :intake C'p  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'3'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C't& h &W C'h% h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'b'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C') +38 h .t\CS.seek 0ml C' '4 +507 h '4 C'V& h &W C'0% h %4 .t\CSV.dial :flush C'N  +37 h  .t\CS.seek 3ml C'S'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'|'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'_'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'2 +76 h .t\CS.seek 0ml C'['4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&H& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&{% h % .t\Forearm.seek :retract M&E'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &^(- +265 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&61 +150 h 1 M&C% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:18 #\Preparing to sleep until Wed Oct 03 06:02:18 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST03-Oct-18 #\Continuing at 06:02CEST03-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:38:09CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST03-Oct-18" +235184 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0109h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *9'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*#'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*c% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*U% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*B % +87 h % F*:% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*d% h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +21 h F*T% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*1  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*+ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*K  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*?  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*L% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*G  +108 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*''\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +21 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*t  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*3  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*/  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0111h25ml.pcr F*b% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *N'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*_'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*8  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +64 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*w  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *C' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ARV.dial :carrier F*A  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *y'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*:  +21 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*d I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*e% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*] n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.53ml F*v' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*T  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +108 h  ."MFBPCR"delay 7 seconds +700 F*P% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*\\'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*`' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*1'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0113h25ml.pcr F*k% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +21 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*3 % +87 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F**'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*)  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ARV.dial :carrier F*H  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*w  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *}'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*E n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*C' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*& % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*9 n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*#  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*c' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*O  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*A  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*D % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0116h25ml.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +21 h F*~% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +21 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*T  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *I'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +31 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*1'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*+  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *F'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*;% h %V ."MFBPCR"\AS.seek 0.5ml F*!'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*x n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*3  +22 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*< n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*q  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.53ml F**' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F*i'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +22 h  ."MFBPCR"delay 7 seconds +700 F*0% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*2' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*_  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*8% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0118h25ml.pcr F*R% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*a % +86 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*$% h %n ."MFBPCR"\AS.seek 0.82ml F*C'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*&  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*X  +64 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*(  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +86 h % F*\ % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*l'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*M% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ARV.dial :carrier F*@  +10 h  F*I% h %V ."MFBPCR"\AS.seek 0.5ml F*P'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*z % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*>  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*1'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*+  +22 h  ."MFBPCR"delay 7 seconds +700 F*K% h %< ."MFBPCR"\AS.seek 0.594ml F*F'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*% n +22 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*; % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*n @@T ."MFBPCR"\ASV.dial :SPEdelivery F*!  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*p  +151 h F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :ASV F*3 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*/  +43 h  F*t% h % ."MFBPCR"\AS.seek 0.62ml F*<'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *q'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*b'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *V' +423 h ' ."MFBPCR"\ATV.dial :air3 F**% h % ."MFBPCR"\AS.seek 0.53ml F*)' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*i% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*|' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*S% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*_ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*8% h % zG ."MFBPCR"\AS.seek 0.365ml F*2' +7161 h ' ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*{% h % ."MFBPCR"\AS.seek 0.579ml F*r' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %  ."MFBPCR"\AS.seek 0.604ml F*w'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*$  +22 h  ."MFBPCR"\AS.seek 0ml F*}'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *X'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*4 % +108 h % F*C% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*9' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*('\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*\ % h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*#' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*5 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*c  +21 h  ."MFBPCR"\AS.seek 0ml F*O'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*A  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*M n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *l'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*W n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *y'R +202 h 'R ."MFBPCR"\SPE.coast F*:  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*B n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*d  +43 h  F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*T  +43 h  F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *@': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*I  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *P'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*o I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *`': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*-  +65 h  F*z% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *k'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*>% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *+'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*K  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *1': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*% n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *g'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*F n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*?  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*L % +43 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*.% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %0 ."MFBPCR"\AS.seek 0.985ml F*G' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*'% h % ."MFBPCR"\AS.seek 0.97ml F*x'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*3 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *t'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*q  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *b' +9988 h ' ."MFBPCR"\ATV.dial AIV F** I +43 h I ."MFBPCR"\AS.seek 0.02ml F*V' +9880 h ' ."MFBPCR"\ATV.dial :waste F*0 n +129 h n ."MFBPCR"\AS.seek 0ml F*i'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*)% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*a  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*$  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*X'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*f  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' @CEST1538431234.71 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *l'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*y % +108 h % ."MFBPCR"\AS.seek 0ml F*B'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538539339.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )d h  C' 'e h  P% %~ h  F* *] h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *"% h %V .t\AS.configure ASconfig S)T% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'v& S)#.0X h  S)k  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M&/ .t\Elbow.jog 1500 M&K* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'+'4 S)#.0X 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:39:11CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #11) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C''& h & .tHand.open M&G1 +150 h 1 M&3& h &_ M&x% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &/(- +166 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +90 h 'η .tHand.close M& 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:39:11CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'n& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&d% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(e% h %X A(~. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (](~ +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*" % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *T' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (v& h &~ M&m& h &- M&I% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&@& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &J'н +89 h 'н A(P& h &~ .t\SE.seek 12.71 Pucks below Top Plate A(o' +137 h ' A( & h &~ M&\\& h & .t\Elbow.seek 3 M&( +6 h ( M&& h & A(& h &~ .t\Forearm.seek 3 + 300 counts M&z'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&-& h & A(>'X M&k% h %- .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&F(ӷ +46 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C';(W +416 h (W .tHand.open M&!1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)L+ +6 h + S) % h % C''& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +350 h ' .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h % .t\CS.seek 6ml C':' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'! +150 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b '`' +710 h ' .t\CSV.dial CSR C'  +151 h  C''% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +7 ']% h %  .t\CS.seek 4.5ml C'%'` +859 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'L  +38 h  .t\CS.seek 1.275ml C'3' +919 h ' #tHeating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'x   '9 h 9d +100 C't9 h 9d +100 C'9 h 91d +100 C'T9 h 9J d +100 C'G9 h 9bd +100 C'q9 h 9zd +100 C'9 h 9d +100 C'.9 h 9d +100 C'Z9 h 9 d +100 C'*9 h 9$d +100 C'b9 h 9(d +100 C'/9 h 9 ,d +100 C'<9 h 9$0d +100 C'p9 h 9<4d +100 C'V9 h 9S8d +100 C'i9 h 9kd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'w9 h 9d +100 C'69 h 9d +100 C'}9 h 9d +100 C'$9 h 9d +100 C'49 h 9d +100 C'9 h 9d +100 C'C9 h 9 d +100 C'R9 h 9 d +100 C'E9 h 96d +100 C'&9 h 9Ld +100 C'X9 h 9bd +100 C's9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'f9 h 9d +100 C'99 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'(9 h 9d +100 C'c9 h 9(d +100 C'O9 h 9>d +100 C'\ 9 h 9Td +100 C'U9 h 9id +100 C'u9 h 9d +100 C'A9 h 9d +100 C'59 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C')9 h 9d +100 C'y9 h 9  d +100 C'M9 h 9 d +100 C'9 h 9 *d +100 C'^9 h 9 ?d +100 C'[9 h 9 Td +100 C'=9 h 9 j d +100 C'W9 h 9 $d +100 C'D9 h 9 (d +100 C'l9 h 9 ,d +100 C'9 h 9 0d +100 C'd9 h 9 4d +100 C'e9 h 9 8d +100 C'~9 h 9 9 h 9 kd +100 C'!9 h 9 ~d +100 C':9 h 9 d +100 C' 9 h 9 d +100 C''9 h 9 d .t\CH.hold 5:00,85C C'`   P h  +30000  C']9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'L @ +113 h @ .t\CSV.dial :air C'% @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C't  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'T  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'G @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'q'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'.  +19 h C'Z% h %4 .t\CS.seek 5ml C'*'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'b  +37 h C'/% h %p .t\CS.seek 7ml C'<'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'p @ +38 h @ .t\CS.seek 10ml C'V' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'i ` +132 h ` #tLysing Complete C' & h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&% h %\  A(h% h %X A(_. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (0( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (+& h &  M&& h &ӷ M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &|( +143 h ( M&8& h & A(H& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Q' +89 h ' A(2& h &  .t\SE.seek 6.71 Pucks below Top Plate A(a' +196 h ' M&S& h & A(j& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&r, +7 h , .t\Elbow.reconfigure TightElbow A(Y- M&$&.,(\ #b h -+ .t\SE.seek 7.04 Pucks below Top Plate A(#' +27 h ' M&% h % A({& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&w'; +21 h '; M&6& h & A(}& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +22 h ' .t\SE.seek 8.04 Pucks below Top Plate A(4'w +36 h 'w M&& h & M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +90 h '\  .t\Elbow.seek CC M&E(ӷ +142 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(f& h &  A(9& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &c( +143 h ( M&O& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &U' +92 h ' .t\Elbow.hold M&u, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &A1 +150 h 1 M&5% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'; +21 h '; M&)& h & A(y& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &M' +22 h ' A(% h %w ."downElevator"\SE.seek :down A(^'X +100 ([% h % M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +82 h 'X ."downElevator"\SE.coast A(5 +7 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'D @ +19 h @ .t\CS.seek 10ml C'l' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'd { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&~1 +2 h 1 M&& h & M&"% h %\  .t\Elbow.seek FlushPuck::Garage M&,(- +165 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&n/ +150 h / M&@& h &- M&% h %η .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +265 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'B(W +416 h (W C' & h &W .tHand.open M& 1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%-% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%J  +37 h  .t\PS.seek 0ml P%k' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%K' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'o  P%z  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%>' +2610 h ' .t\PSV.dial :air P%!  +38 h  .t\PS.seek 2.5ml P%:' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%'' +2610 h ' .t\PSV.dial :air P%`  +37 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :mixloop P%3 +38 h .t\PS.seek 0ml P%L' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&%& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&.% h % .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &q(- +264 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&p1 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'i& h & M& % h %\  A(% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (_(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (+& h &~ M&& h &- M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &8( +166 h ( M&H& h & A(2& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &F'н +90 h 'н A(S& h &~ .t\SE.seek 11.71 Pucks below Top Plate A(j'Ɛ +145 h 'Ɛ A(1& h &~ M&Y& h & .t\Elbow.seek 3 M&( +7 h ( M&{& h & A(6& h &~ .t\Forearm.seek 3 + 300 counts M&}'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&C& A(Q'X h & M&|% h %- .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +55 h 'X ."downElevator"\SE.coast A(5 +88 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +90 h ' .t\CC.seek :closed C'#(W +416 h (W .tHand.open M&$1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'R  +151 h  .t\CSV.dial CTV C';  +19 h  .t\CS.seek 5ml C'f'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'9' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%O' +2610 h ' .t\PSV.dial :mixloop P%\ +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%s  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%E +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +150 h  .t\PS.seek :empty P%5' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C')  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'y @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'( ` +19 h ` .t\CSV.dial :air C'c @ +38 h @ .t\CS.seek 10ml C'[' +2647 h ' .t\CSV.dial CTV C'=  +38 h  .t\CS.seek 0ml C'D'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'd  +150 h C'e& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&~% h %\  A(% h %X A(". h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (U( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&4& h &ӷ M&M% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &m( +142 h ( M&I& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &0' +90 h ' A(V& h &  .t\SE.seek 7.71 Pucks below Top Plate A(u' +184 h ' M&n& h & A(@& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&W, +6 h , .t\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b .t\SE.seek 8.04 Pucks below Top Plate A(7'w +27 h 'w M&\\% h % A(,& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &P'; +20 h '; M&& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A(v'f +37 h 'f M&& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&J(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&k' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'o( +416 h ( A(& h &  A(& h &  M&K& h &ӷ M&!% h % .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +143 h ( M&:& h & A(`& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &'' +92 h ' .t\Elbow.hold M&3, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &]1 +150 h 1 M&%% h % A(t& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'; +21 h '; M&G& h & A(?& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &g' +22 h ' A(B% h %f ."downElevator"\SE.seek :down A('X +100 (.% h % M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +72 h 'X ."downElevator"\SE.coast A(5 +18 h '\  .t\SC.coast .\PSV.dial :air A(6 P%/  +113 h  P%% h % .t\PS.seek 0.499ml P%p' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%x +38 h .t\PRV.dial :air P%Z  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%i  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%h  +18 h  P%+% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%N @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %*' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&21 +2 h 1 M&S& h & M&q% h %\  .t\Elbow.seek FlushPuck::Garage M&1(- +166 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&6'η +91 h 'η .tHand.close M&' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial PSR P%T +113 h P%'% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%/' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*g n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*U'% +336 h ' .t\PSV.dial PSR P%5 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%x' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%Z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%i' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%h' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%+  +38 h  .t\PS.seek 0ml P%p' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%N +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %S'k +2679 h 'k .t\PSV.dial :waste1 P%q  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %Y' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*{ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *3'V +850 h ' .t\PSV.dial :air P%<  +112 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %b' +5106 h ' .t\PSV.dial :air P%6  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %F' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*&  +43 h  F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *|'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*o I +43 h I F*j% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%Q +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +619 h ' ."MFBPCR"\ATV.dial :waste F*$ n +129 h n ."MFBPCR"\AS.seek :empty F*X'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*r  +86 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *;' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F**  +43 h F*w% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *l'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'_  +38 h  C'9% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'L' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  's  +38 h  +113  .t\CBV.dial :waste1 C'E @ +38 h @ .t\CS.seek 0ml C'\ '4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*O  +22 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*)'ݏ +110 h '4 .t\CSV.dial CSR C'y  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'('P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*c  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*='& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*['&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'e' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'"& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +7 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&4' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&n& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'm& h & M&u% h %\  A(% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (0(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (P& h &~ M&& h &- M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &7( +166 h ( M&z& h & A(#& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A(& h &~ ."WCR"\SE.seek 10.71 Pucks below Top Plate A(' +155 h ' A(W& h &~ M&!& h & ."WCR"\Elbow.seek 3 M&( +6 h ( M&`& h & A(J& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&]'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &K/ +100 h '&0 ."MFBPCR"delay 3 seconds +50 h / ."downElevator"\SE.seek :down A(:'X M&t& h & M&?% h %- ."WCR"\Forearm.seek :retract M&B'\ +91 h '\  ."WCR"\Elbow.seek CC M&k(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&%& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &G' +16 ."MFBPCR"\ATV.dial :waste2 F*T % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *''R +32 h ' ."WCR"\CC.seek :closed C'.(W +416 h (W ."WCR"Hand.open M&v1 +111 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +17 h 1 M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)>+ +6 h + S)5% h % C'/& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'Z  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *h'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*x @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*U I +86 h I ."MFBPCR"\AS.seek 0ml F*2'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*q  +43 h  #"MFBPCR"\Swish #1 of 2 F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *{'\  +1032 h '\  F*H% h %\  ."MFBPCR"\AS.seek 0.5ml F*<'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*Y% h %(A ."MFBPCR"\AS.seek 0.6ml F*C'\  +1031 h '\  F*S% h %\  ."MFBPCR"\AS.seek 0.5ml F*b'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*&% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *1' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*3 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*6  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*j  +64 h  ."MFBPCR"\ARV.dial :carrier F*Q  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F**  +21 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *|'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*;  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.53ml F*D' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*E % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.536ml F*s'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*O n +21 h n ."MFBPCR"delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*)  +43 h  ."MFBPCR"\ASV.dial :waste1 F*=  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*d% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254620 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'M  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'" @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'8 @ +75 h @ +50 S)I% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'n% h % ."WCR"\CSV.dial between air and CTV C'a ` +19 h ` ."WCR"\CS.seek 7.288ml C'4' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\\  '  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ',' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'-  +38 h  C'u% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'm @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C' % h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'#' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'z  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'W'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'!  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '@' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'J  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'`% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'K' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'7 @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'?  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C'T ` +56 h ` ."WCR"\CS.seek 7.288ml C'%' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C']  ."WCR"\CSV.dial CTV C'  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ':'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '[' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'^  +151 h C'B& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&v% h %\  A(g% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (/( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Z& h &  M&5& h &ӷ M&G% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &0( +143 h ( M&'& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(>& h &  ."WCR"\SE.seek 8.71 Pucks below Top Plate A(+' +175 h ' M&i& h & A(h& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &U' +26 h ' ."WCR"\Elbow.hold M&p, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(2- M&$&.,(\ #b h -+ ."WCR"\SE.seek 9.04 Pucks below Top Plate A(q'f +27 h 'f M&N% h % A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&H'; +20 h '; M&{& h & A(Y& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &<' +22 h ' ."WCR"\SE.seek 10.04 Pucks below Top Plate A(S'U +37 h 'U M&C& h & M&&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +89 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +143 h (ӷ M&1& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ ."WCR"\Forearm.seek CC M&j' +91 h ' ."WCR"Hand.close M&Q/ +150 h / ."WCR"\CC.seek :open C'( +415 h ( A(& h &  A(F& h &  M&& h &ӷ M&$% h % ."WCR"\Forearm.seek :retract M&r'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &o( +143 h ( M&*& h & A(w& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &R' +92 h ' ."WCR"\Elbow.hold M&;, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &91 +150 h 1 M&|% h % A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&f'; +20 h '; M&_& h & A(E& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &L' +22 h ' A(D% h %U ."downElevator"\SE.seek :down A(A'X +100 (y% h % M&s% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +62 h 'X ."downElevator"\SE.coast A(5 +28 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&1 +2 h 1 M&& h & M&c% h %\  .t\Elbow.seek FlushPuck::Garage M&l(- +166 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&d/ +150 h / M&& h &- M&(% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +264 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'I(W +416 h (W C'}& h &W .tHand.open M&n1 +150 h 1 M&a% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'\\& h &W C'% h % .t\CSV.dial CSR C'4  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'~'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '-'[ +463 h '[ .t\CSV.dial :waste C'u +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2009 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'm% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'z  '  +38 h  +112  .t\CBV.dial :waste1 C'# @ +76 h @ .t\CS.seek 0ml C'!'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'W& h &W C'J% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'`'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'K% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing upper manifold C'T& h &W C't% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C']'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +38 h @ C'% h %p .t\CS.seek 7ml C':'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'^ 'B  +38 h +75 .t\CTV.dial :air C'v  +151 h .t\CS.seek 0ml C'g'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'Z +38 h .t\CS.seek 0ml C'5'4 +506 h '4 C'G& h &W C''% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'>'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'N +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'{& h &W C'Y% h %4 .t\CSV.dial CSR C'C  +150 h  C'&% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'0' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'+ +113 h .t\CTV.dial :intake C'U  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'1' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'6  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'q  +113 h  C'<% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  'b  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'F'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'$% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '*'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +710 h ' ."connect"\CSV.dial CTV C'j  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'3  +76 h  .t\CS.seek 0ml C'9'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'|  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D  'r +38 h +75 .t\CTV.dial :air C'o  +151 h .t\CS.seek 0ml C'y'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +506 h '0 .t\CSV.dial :waste C'\ +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'& h &W C'c% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +38 h @ C')% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CBV C'L .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +506 h '0 .t\CSV.dial :waste C'; +38 h .t\CS.seek 0ml C'd'4 +507 h '4 C'& h &W C'(% h %4 .t\CSV.dial :flush C'O  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'} +76 h .t\CS.seek 0ml C'n'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'\\% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'4'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'8% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '~  +37 h  +113  .t\CBV.dial :waste1 C'u @ +38 h @ .t\CS.seek 0ml C'-'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'm% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C','p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'z  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'#'4 +766 h '4 #tDisinfecting external Intake with kill C'P& h &W C'W% h %4 .t\CSV.dial CSR C'J  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'!'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'K '`  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'?' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'7  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'T'4 +607 h '4 .t\CSV.dial CSR C't  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'%' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C']'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&B& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +7 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&g' +90 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C'( +416 h ( M&G& h &ӷ M&'% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +265 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &>'η +91 h 'η .tHand.open M&h1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&{1 +2 h 1 M&Y& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &C(- +6 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&+/ +150 h / M&U& h &- M&0% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +264 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C'p(W +416 h (W C'.& h &W .tHand.open M&b1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'$% h %4 .t\CSV.dial CSR C'F  +113 h  C'*% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'j'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '3% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'w'[ +463 h '[ .t\CSV.dial :waste C'| +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'9& h &W C'_% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'o .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'E  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'y'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'\  +76 h #t\Flushing upper manifold C's& h &W C'% h %4 .t\CSV.dial :flush C'c  +75 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'S% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f +113 h .t\CTV.dial :air .\CS.seek 0ml C'L'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +506 h '0 .t\CSV.dial :waste C'A +38 h .t\CS.seek 0ml C';'4 +507 h '4 C'/& h &W C'% h %4 .t\CSV.dial :flush C'(  +37 h  .t\CS.seek 3ml C'O'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +37 h @ C'"% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'}'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +7 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'8( +416 h ( M&4& h &ӷ M&% h % .t\Forearm.seek :retract M&~'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:34 #\Preparing to sleep until Fri Oct 05 06:02:34 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST05-Oct-18 #\Continuing at 06:02CEST05-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:40:42CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST05-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0309h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*z  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*J% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*`'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*7  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +65 h  F*t% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +87 h % F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*<% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *F'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F**  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*3  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*|' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*w  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F*_'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +22 h n ."MFBPCR"delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*c' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*S% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0311h25ml.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*=  +21 h ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*l% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*a % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Z  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*M  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*G I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*k n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*^' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*h  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*2  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*{'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*0  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*5  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0313h25ml.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*6  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F**'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*9  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*|  +64 h  F*D% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F*y % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *S' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*f% h % ."MFBPCR"\AS.seek 0.81ml F*)'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*Q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*a  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *['Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*~ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*m% h %Fj ."MFBPCR"\AS.seek 0.524ml F*-' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*W  +107 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*J'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*P n +21 h n ."MFBPCR"delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.6ml F*!'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*`  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*7  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*@'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0316h25ml.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*%  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*v% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*I'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*i  +64 h  F*^% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *5' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*<'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ARV.dial :carrier F*w  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*_'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*r n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*y  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*c n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*S' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*x  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*)' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*=  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*d n +22 h n ."MFBPCR"delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.6ml F*O'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\\% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0318h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*4  +22 h ."MFBPCR"\ATV.dial :air3 F*p % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*n'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +21 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*e% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +31 h  ."MFBPCR"\ATV.dial :air3 F*! % +87 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*^  +42 h  ."MFBPCR"\ARV.dial :carrier F*g  +11 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*5' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*N % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.536ml F*q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*b n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*j' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*3  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*| @@T ."MFBPCR"\ASV.dial :SPEdelivery F*_  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*E  +150 h F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\ ' +424 h ' ."MFBPCR"\ATV.dial :ASV F*y n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*s% h % ."MFBPCR"\AS.seek 0.62ml F*c'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*f n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *x'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*L'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b */' +424 h ' ."MFBPCR"\ATV.dial :air3 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.71ml F*d' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*O% h % Ű ."MFBPCR"\AS.seek 0.72ml F*l' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*\\% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *4' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +21 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*Q' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*m% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*n' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*e  +22 h  ."MFBPCR"\AS.seek 0ml F*['V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*-% h %V ."MFBPCR"\AS.seek 0.01ml F*W' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +21 h F*z% h % ."MFBPCR"\AS.seek 0.58ml F*P' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h % q ."MFBPCR"\AS.seek 0.605ml F*K'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F*`% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*? n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*7'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *%' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *@'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +202 h 'R ."MFBPCR"\SPE.coast F*M  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*v'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*Z n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*G  +43 h  F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *:' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*k I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*h  +43 h  F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *g': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*2  +65 h  F*i% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Y n +64 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *&' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*{  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*>  +65 h  F*N% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *C'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*5 n +64 h n F*6% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *.' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*< I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *q': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*b n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*$ n +151 h n ."MFBPCR"\AS.seek 0ml F*F'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F**  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*H % +43 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*j% h % ."MFBPCR"\AS.seek 0.96ml F*w'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h %0 ."MFBPCR"\AS.seek 0.985ml F*D' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +21 h F*o% h % ."MFBPCR"\AS.seek 0.97ml F*r'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*y I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *s'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *f'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*x  +43 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *=' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *L' +9988 h ' ."MFBPCR"\ATV.dial AIV F*) I +43 h I ."MFBPCR"\AS.seek 0.02ml F*/' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*R'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*A% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*d % +43 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*\\'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*p  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +31 h  ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*-% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +21 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +21 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' @CEST1538604049.83 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *@'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*B % +107 h % ."MFBPCR"\AS.seek 0ml F*Z'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast P%6 M&5 +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538712154.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )G h  C' 'V h  P% %v h  F* *k h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *:  h  .t\SPE.configure SPEconfig F*\ 2 *'% h %V .t\AS.configure ASconfig S)h% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% C'N  h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &U'\ h ."rdySampler"\CSV.dial :waste1 C'5 h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)6 h  S)C  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %<' +20 h '\  ."closeHand"Hand.close M&./ .t\Elbow.jog 1500 M&* +18 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml ."rdySampler"\SS.home.seek 0ml C'#d @b S)#.0X 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:41:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #12) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'o& h & .tHand.open M&D1 +150 h 1 M&y& h &_ M&r% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&s& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &f'η +91 h 'η .tHand.close M&c/ +150 h / M&x& h &- M&S% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +264 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'A(W +416 h (W C'/& h &W .tHand.open M&R1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'"& h &W C'(% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'O'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'l% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'\\ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'p  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'4 .t\CBV.dial CSV C'1  h +75 .t\CTV.dial :intake C'a  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Q  +75 h #t\Flushing upper manifold C'~& h &W C'm% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'e'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +37 h @ C'[% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'-'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C', +37 h .t\CS.seek 0ml C'z'4 +507 h '4 C' & h &W C'#% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 3ml C'W'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'P% h %0 .t\CS.seek 5ml C'`'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&%/ +150 h / .t\CC.seek :open C'M( +416 h ( M& & h &ӷ M&B% h % .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&u& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&X1 +150 h 1 M&}% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:41:43CEST30-Aug-18\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:41:39CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*Z% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'|& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&G% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(V% h %X A(v. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (k(~ +7 F*:% h %&K ."readyMFB"\ATV.dial :waste2 F*' % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *h' +193 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (^& h &~ M&I& h &- M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &i( +166 h ( M&g& h & A(Y& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & 'н +90 h 'н A(& h &~ .t\SE.seek 9.71 Pucks below Top Plate A(+' +166 h ' A(&& h &~ M&{& h & .t\Elbow.seek 3 M&( +6 h ( M&& h & A(>& h &~ .t\Forearm.seek 3 + 300 counts M&N'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &5/ +150 h / ."downElevator"\SE.seek :down A(6'X M&0& h & M&C% h %- .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.seek CC M&$(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&_1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)H+ +6 h + S)F% h % C'o& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +100 ."CSpush"\CSV.dial :air C'8 @ +38 h @ ."CSpush"\CS.reconfigure CSfast .\CS.seek 0ml C'#d @b ''4 +6 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S)5 +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'm9 h 9Td +100 C'a9 h 9kd +100 C' 9 h 9d +100 C'9 h 9d +100 C'[9 h 9d +100 C'e9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'-9 h 9 d +100 C'9 h 9 d +100 C'~9 h 96d +100 C'9 h 9Ld +100 C' 9 h 9bd +100 C'#9 h 9xd +100 C'n9 h 9d +100 C'z9 h 9d +100 C'!9 h 9d +100 C'P9 h 9d +100 C'J9 h 9d +100 C'W9 h 9d +100 C'K9 h 9d +100 C'9 h 9(d +100 C'79 h 9>d +100 C'`9 h 9Td +100 C't9 h 9id +100 C',9 h 9d +100 C'9 h 9d +100 C'?9 h 9d +100 C'%9 h 9d +100 C'd9 h 9d +100 C'A9 h 9d +100 C'B9 h 9  d +100 C'9 h 9 d +100 C'=9 h 9 *d +100 C'u9 h 9 ?d +100 C' 9 h 9 Td +100 C'X9 h 9 j d +100 C' 9 h 9 $d +100 C'T9 h 9 (d +100 C'@9 h 9 ,d +100 C'Z9 h 9 0d +100 C'G9 h 9 4d +100 C'V9 h 9 8d +100 C'v9 h 9 9 h 9 d +100 C'09 h 9 d +100 C'i9 h 9 d +100 C' 9 h 9 d +100 C'C9 h 9 d +100 C'99 h 9 d +100 C'9 h 9 d +100 C'N9 h 9 d +100 C'+9 h 9 1d +100 C'9 h 9 Dd +100 C'.9 h 9 Xd +100 C'69 h 9 kd +100 C'_9 h 9 ~d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'o9 h 9 d .t\CH.hold 5:00,85C C'U   P h  +30000  C'b9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'3'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'H @ +113 h @ .t\CSV.dial :air C'k @ +38 h @ .t\CS.seek 5ml C'<'p +1372 h 'p .t\CSV.dial CTV C's  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'h  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'D @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'x'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'S +113 h .t\CBV.dial CSV C'j  +19 h C']% h %4 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'L  +38 h C'% h %p .t\CS.seek 7ml C'c'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'E @ +38 h @ .t\CS.seek 10ml C'/' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'R ` +132 h ` #tLysing Complete C';& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&"% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&l& h &ӷ M&(% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&\\& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 9.71 Pucks below Top Plate A(p' +166 h ' M&O& h & A(1& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &4' +26 h ' .t\Elbow.hold M&Q, +6 h , .t\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b .t\SE.seek 10.04 Pucks below Top Plate A('U +27 h 'U M&m% h % A(a& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M& '; +20 h '; M&& h & A([& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &e' +22 h ' .t\SE.seek 11.04 Pucks below Top Plate A('D +37 h 'D M&& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&~(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'z( +415 h ( A(!& h &  A(P& h &  M&J& h &ӷ M&W% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&7& h & A(`& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &t' +92 h ' .t\Elbow.hold M&,, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&?% h % A(%& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &d'; +20 h '; M&A& h & A(B& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(=% h %D ."downElevator"\SE.seek :down A(u'X +100 ( % h % M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +52 h 'X ."downElevator"\SE.coast A(5 +38 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'T @ +19 h @ .t\CS.seek 10ml C'@' +6 h ' .t\CSV.dial between air and CTV C'Z ` +19 h ` .t\AIV.dial :bypass C'G { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'V& h & .tHand.open M&v1 +2 h 1 M&:& h & M&'% h %\  .t\Elbow.seek FlushPuck::Garage M&}(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&|/ +150 h / M&g& h &- M&Y% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'8(W +416 h (W C'&& h &W .tHand.open M&51 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%> +151 h P%0% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%C' +1597 h ' .t\PSV.dial PSR P%9 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%N  C'+  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%.  h .t\PS.seek 0ml P%6' +2610 h ' .t\PSV.dial :air P%_  +37 h  .t\PS.seek 2.5ml P%M' +2610 h ' .t\PSV.dial :mixloop P%F +38 h .t\PS.seek 0ml P%o' +2609 h ' .t\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :mixloop P%r +37 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&k& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&D/ +150 h / .t\CC.seek :open C'<( +416 h ( M&S& h &ӷ M&j% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &)'η +91 h 'η .tHand.open M&E1 +150 h 1 M&y% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'R& h & M&;% h %\  A("% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&l& h &- M&(% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &\\( +166 h ( M&& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &$'н +89 h 'н A(O& h &~ .t\SE.seek 8.71 Pucks below Top Plate A(1' +175 h ' A(& h &~ M&q& h & .t\Elbow.seek 3 M&m( +7 h ( M&a& h & A(& h &~ .t\Forearm.seek 3 + 300 counts M&['- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&-& A('X h & M&% h %- .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.seek CC M&4(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +59 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +415 h (W .tHand.open M&e1 +150 h 1 M&Q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'!'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'P' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'J'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%7' +2610 h ' .t\PSV.dial :mixloop P%` +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%#  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%~ +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%?' +2610 h ' .t\CBV.dial :waste1 C'% @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'A  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'B @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'=  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'K ` +18 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'X  +38 h  .t\CS.seek 0ml C'T'4 +2647 h '4 .t\CBV.dial :waste1 C'Z @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'V& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&v% h %\  A(:% h %X A('. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (t( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (d& h &  M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &I( +142 h ( M&2& h & A(u& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(/& h &  .t\SE.seek 10.71 Pucks below Top Plate A(,' +155 h ' M&|& h & A(g& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &Y' +25 h ' .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ .t\SE.seek 11.04 Pucks below Top Plate A('D +26 h 'D M&{% h % A(}& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'; +21 h '; M&z& h & A(&& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &5' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(^'3 +36 h '3 M&>& h & M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M& (ӷ +143 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.seek CC M&C' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'+( +416 h ( A(& h &  A(.& h &  M&& h &ӷ M&_% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &F( +142 h ( M&M& h & A(U& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &o' +93 h ' .t\Elbow.hold M&r, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &b1 +150 h 1 M&k% h % A(s& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &\ '; +20 h '; M&D& h & A(*& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +23 h ' A(8% h %3 ."downElevator"\SE.seek :down A(S'X +100 (j% h % M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +113 h  P%3% h % .t\PS.seek 0.499ml P%E' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%y +37 h .t\PRV.dial :air P%]  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%R  +38 h .t\CBV.dial AIV C';  +37 h .t\PRV.dial between CBV and air P%"  +19 h  .t\PRV.dial :air P%  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %l' +250 h ' .t\PRV.dial :waste2 P%( @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %)' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&O& h & M&x% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&c/ +150 h / M&& h &- M&-% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'1& h &W .tHand.open M&m1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  C'<& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%e @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%Q  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%@  +38 h  .t\PS.seek 0ml P%!' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%P +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%W  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %`'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%#  +75 h .tdelay 2 seconds +200 P%~% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%7  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%A  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%B  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %=' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%K +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%J' +2610 h ' .t\PSV.dial :air P%X  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Z +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%V  +75 h  .t\PS.seek 2.5ml P%v' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%: +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%'  +75 h P%d% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%2% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %u'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'/ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %4' +1313 h ' .t\PSV.dial :air P%|  +38 h  .t\PS.seek 2.5ml P%g' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%n' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '{& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'}& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*z @@T .t\AIV.dial :bypass F*&  .t\PSV.dial PSR P%> +22 h  ."MFBPCR"delay 20 seconds +91 h P%0% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*I I +129 h I F*,% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Y'6 +65 .t\PSV.dial :waste1 P%i  +38 h  .t\PS.seek 0ml P%9' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%5 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*N  +22 h ' .t\PSV.dial PSR P% +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*_ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +38 h  .t\PS.seek 0ml P%C' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*b% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *k' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%s +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%D' +217 ."MFBPCR"\ATV.dial :AIV F** I +43 h I F*8% h % ."MFBPCR"\AS.seek 0.469ml F*6' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%j' +2609 h ' .t\PSV.dial PSR P%h +113 h P%o% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%\ ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%^  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*39 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*S% h % ."MFBPCR"\AS.seek 0.041ml F*t'% +336 h ' .t\PSV.dial PSR P%? +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%y' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%] +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%R' +2610 h ' .t\PSV.dial PSR P%; +113 h P%"% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%E' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%l' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %O'k +2678 h 'k .t\PSV.dial :waste1 P%x  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %q' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*a I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *r'V +850 h ' .t\PSV.dial :air P%c  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%- +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %L' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %$' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*1% h %Q ."MFBPCR"\AS.seek 0.01ml F*m' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %<' +618 h ' ."MFBPCR"\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*Q  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*)  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *@'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'P% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'#  '!  +38 h  +112  .t\CBV.dial :waste1 C'~ @ +38 h @ .t\CS.seek 0ml C'`'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*7  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*A'ݏ +110 h '4 .t\CSV.dial CSR C'B  +38 h  C'%% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'K'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*=% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*X'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*J n +108 h n F*Z% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'V' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C':'4 +2647 h '4 C''& h &W #tGaraging FlushPuck in Collection Clamp M&d& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'u( +416 h ( M&|& h &ӷ M&4% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +264 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +91 h 'η .tHand.open M&>1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'I& h & M&,% h %\  A(i% h %X A(f. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&9& h &- M&5% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&N& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +89 h 'н A(.& h &~ ."WCR"\SE.seek 7.71 Pucks below Top Plate A(Y' +185 h ' A( & h &~ M&_& h & ."WCR"\Elbow.seek 3 M&( +6 h ( M&U& h & A( & h &~ ."WCR"\Forearm.seek 3 + 300 counts M&b'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +70 h '&0 ."MFBPCR"delay 3 seconds +80 h / ."downElevator"\SE.seek :down A(s'X M&M& h & M&*% h %- ."WCR"\Forearm.seek :retract M&8'\ +91 h '\  ."WCR"\Elbow.seek CC M&C(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +35 ."MFBPCR"\ATV.dial :waste2 F*k % +14 h (ӷ M&F& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +29 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +62 h ' ."WCR"\CC.seek :closed C'j(W +416 h (W ."WCR"Hand.open M&^1 +82 h 'R ."MFBPCR"\ATV.dial :air2 F*\  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *3' +47 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +6 h + S)?% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C']  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % k ."MFBPCR"\AS.seek 0.779ml F*0'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *I' +4416 h ' ."MFBPCR"\ATV.dial :waste F*, n +128 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*&% h % ."MFBPCR"\AS.seek 0.579ml F*N' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %  ."MFBPCR"\AS.seek 0.604ml F*g'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*_% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*U' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*n  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b **'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*F' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*D n +107 h n ."MFBPCR"\ASV.dial :wasteR F*k% h %  ."MFBPCR"\AS.seek 0.09ml F*Y' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*b  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*s% h % ."MFBPCR"\AS.seek 0.12ml F*8' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +22 h % F*u% h % ."MFBPCR"\AS.seek 0.13ml F*\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +21 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*?' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h % ."MFBPCR"\AS.seek 0.255ml F*o'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*6' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*; n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*"  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*y @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*t I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*3'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*x  +43 h  #"MFBPCR"\Swish #1 of 2 F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *a'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*c'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*q% h %(A ."MFBPCR"\AS.seek 0.6ml F*-'\  +1031 h '\  F*O% h %\  ."MFBPCR"\AS.seek 0.5ml F*L'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*r % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0509h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*1  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*)  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*P% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*T' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*~ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*B% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*7 n +21 h n ."MFBPCR"delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*J' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 F*X  +64 h ."MFBPCR"\ARV.dial :air1 F*Z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254649 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'\\ @ +75 h @ +50 S)2% h % ."WCR"\CBV.dial between CSV and waste2b C'[  +94 h  C'|% h % ."WCR"\CSV.dial between air and CTV C'p ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'4  '{  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'v'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'>  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '}' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'0  +38 h  C',% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'f' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'I @ +76 h @ +50 S)i% h % ."WCR"\CBV.dial between CSV and waste2b C'5  +94 h  C'&% h % ."WCR"\CSV.dial between air and CTV C'9 ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'N  '.  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'_  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'g' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'n  +37 h  C'U% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'*  +94 h  C'D% h % ."WCR"\CSV.dial between air and CTV C'k ` +56 h ` ."WCR"\CS.seek 7.288ml C'F' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'b  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 's'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'Y  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'u  +151 h C'8& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&^% h %\  A(% h %X A(\ . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (]& h &  M&?& h &ӷ M&h% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&o& h & A(;& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &"' +89 h ' A(6& h &  ."WCR"\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ M&R& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &t' +25 h ' ."WCR"\Elbow.hold M&E, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+Ɛ ."WCR"\SE.seek 12.04 Pucks below Top Plate A(x'3 +27 h '3 M&(% h % A(3& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'; +21 h '; M&a& h & A(q& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +22 h ' ."WCR"\SE.seek 13.04 Pucks below Top Plate A(O'" +36 h '" M&-& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +90 h '\  ."WCR"\Elbow.seek CC M&r(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&1' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'l( +416 h ( A(& h &  A($& h &  M&m& h &ӷ M&e% h % ."WCR"\Forearm.seek :retract M&Q'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &+( +142 h ( M&)& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &P1 +150 h 1 M&% h % A(W& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &#'; +21 h '; M&& h & A(~& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +22 h ' A(T% h %" ."downElevator"\SE.seek :down A('X +100 (B% h % M&!% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +33 h 'X ."downElevator"\SE.coast A(5 +56 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&%1 +2 h 1 M&=& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&@(- +166 h (- M&A& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&Z'η +91 h 'η .tHand.close M&G/ +150 h / M&J& h &- M&K% h %η .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'2(W +416 h (W C'[& h &W .tHand.open M&|1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'{& h &W C'4% h % .t\CSV.dial CSR C'  +38 h  C'>% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'v'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '0'[ +462 h '[ .t\CSV.dial :waste C', +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'f& h &W C'I% h %4 .t\CSV.dial :flush C'i  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '5'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'9% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'N  .t\CTV.dial CSV C'.  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'_'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C' % h %4 .t\CSV.dial :flush C'n  +75 h  .t\CS.seek 5ml C'U'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'g @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'*  +75 h  .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing upper manifold C'k& h &W C'M% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'b'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +37 h @ C'Y% h %p .t\CS.seek 7ml C's'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u '8  +38 h +75 .t\CTV.dial :air C'^  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +506 h '0 .t\CSV.dial :waste C'] +38 h .t\CS.seek 0ml C'?'4 +507 h '4 C'h& h &W C'o% h %4 .t\CSV.dial :flush C';  +37 h  .t\CS.seek 3ml C'6'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +37 h @ C'R% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +506 h '0 .t\CSV.dial :waste C'3 +76 h .t\CS.seek 0ml C'S'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'a& h &W C'q% h %4 .t\CSV.dial CSR C'-  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'"'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C't  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'x  +113 h  C'c% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'E'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV C'L  .t\CTV.dial CSV C'j  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'$'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'm  +38 h  C'e% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ')'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'1  +38 h  .t\CBV.dial :waste2b C'r  +75 h  .t\CS.seek 0ml C'P'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'W% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'T  +37 h +76 .t\CTV.dial :air C'+  +150 h .t\CS.seek 0ml C'B'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'%'4 +507 h '4 #t\Flushing upper manifold C'=& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'#'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +37 h @ C'A% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C'J& h &W C'K% h %4 .t\CSV.dial :flush C'7  +38 h  .t\CS.seek 3ml C'Z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +38 h @ C':% h %0 .t\CS.seek 5ml C'@'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'd'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +507 h '0 .t\CSV.dial :waste C'[ +75 h .t\CS.seek 0ml C'|'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'p& h &W C'{% h %4 .t\CSV.dial :flush C'4  +37 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'\\% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'v  +38 h  +113  .t\CBV.dial :waste1 C', @ +37 h @ .t\CS.seek 0ml C'0'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'f& h &W C'I% h %4 .t\CSV.dial :flush C'i  +76 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'9% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  +38 h  .t\CBV.dial :waste2b C'.  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C' % h %4 .t\CSV.dial CSR C'  +38 h  C'n% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'_'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'g' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'U  +37 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'*' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +606 h '4 .t\CSV.dial CSR C'M  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'F' +1372 h ' .t\CSV.dial CBV C'Y +113 h .t\CS.seek 0ml C'b'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&8& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +7 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'\ ( +416 h ( M&h& h &ӷ M&o% h % .t\Forearm.seek :retract M&;'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &?(- +264 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'(& h & .tHand.open M&a1 +2 h 1 M&q& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &-(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.seek :garage M&"'η +91 h 'η .tHand.close M&/ +150 h / M&t& h &- M&% h %η .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'E(W +416 h (W C'L& h &W .tHand.open M&j1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'm& h &W C'e% h %4 .t\CSV.dial CSR C'$  +113 h  C')% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'1'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +2010 h '4 #t\Purging external Intake C'P& h &W C'% h %4 .t\CSV.dial :flush C'T  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'+ +75 h .t\CTV.dial :intake C'~  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'B'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'`  +75 h #t\Flushing upper manifold C'!& h &W C'=% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +37 h @ C'O% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# +113 h .t\CTV.dial :air .\CS.seek 0ml C'H'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'J% h %4 .t\CSV.dial :flush C'K  +38 h  .t\CS.seek 3ml C'7'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C''% h %0 .t\CS.seek 5ml C':'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +507 h '0 .t\CSV.dial :waste C'd +75 h .t\CS.seek 0ml C'['4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&l& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &p(ӷ +6 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&|' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'\\( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &,(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &I'η +91 h 'η .tHand.open M&i1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  #t\Completed [mbaPhase] begun 06:02:49 #\Preparing to sleep until Sun Oct 07 06:02:49 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:02CEST07-Oct-18 #\Continuing at 06:02CEST07-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:115\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:43:10CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:02CEST07-Oct-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0509h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*N  +21 h ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*n'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F*U'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F**  +65 h  F*M% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +31 h  ."MFBPCR"\ATV.dial :air3 F*] % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*a' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *3'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0511h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*x  +10 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*)  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*b' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*r  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*1% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +108 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*`'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*!  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*B  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*%  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*O% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*A % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0511h25ml.pcr F*H% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *K'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*@% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*{  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +64 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*f  +32 h  ."MFBPCR"\ATV.dial :air3 F*p % +86 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +21 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*N'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*5% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0513h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*k% h %V ."MFBPCR"\AS.seek 0.5ml F*D'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*]  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *2'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*h I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*/% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*C n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*;'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*(% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*?  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*x'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*3 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0513h25ml.pcr F*-% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*j% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +21 h F*b% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +31 h  ."MFBPCR"\ATV.dial :air3 F*B % +87 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*#% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +21 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0516h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*l  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +22 h ."MFBPCR"delay 2 seconds +200 F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*v n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*0' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*9 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.53ml F*z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*. % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*N'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*n  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*g'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*k % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0516h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*F  +22 h ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +21 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +21 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +65 h  F*u% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*c'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*x'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*L% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0518h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *+'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*B  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *='Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*y  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*A' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*X  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*G n +21 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*:  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Z  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*>'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0518h25ml.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*|'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*V% h %n ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*.  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +64 h  F*z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*g'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*Y% h %m ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0520h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*" I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*3 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.536ml F*x'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*j n +22 h n ."MFBPCR"delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.65ml F*1' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*P% h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0520h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*~  +151 h F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *`' +423 h ' ."MFBPCR"\ATV.dial :ASV F*B n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*%  +43 h  F*!% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*# n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *y'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*H'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*O % +43 h % F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*G' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*:' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*7% h % Ű ."MFBPCR"\AS.seek 0.72ml F*@' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*Z n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*{% h % zG ."MFBPCR"\AS.seek 0.365ml F*d' +7161 h ' ."MFBPCR"\ATV.dial :waste F*l n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*>% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %  ."MFBPCR"\AS.seek 0.604ml F*f'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*I% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*|' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*9 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +22 h  ."MFBPCR"\AS.seek 0ml F* 'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *5'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*z % +108 h % F*0% h %V ."MFBPCR"\AS.seek 0.01ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*N% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F*U% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*n' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F** n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *M'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *F' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Y n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *g'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*D n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*^'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*] n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*h  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *s' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*C I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*R% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *('Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*q n +65 h n F*;% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*a  +43 h  F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*3% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *-'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*? n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *L' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*c I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *x': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*j n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *m'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*e n +151 h n ."MFBPCR"\AS.seek 0ml F*$'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*)  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*1% h % ."MFBPCR"\AS.seek 0.96ml F* '0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*T' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*+% h % ."MFBPCR"\AS.seek 0.97ml F*Q'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*B I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *!'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *#'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *='R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*y  +43 h  F*O% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *H' +9988 h ' ."MFBPCR"\ATV.dial AIV F*A I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +43 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *7'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*Z% h %Z ."MFBPCR"\AS.seek 0.81ml F*{'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*d'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*>% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +21 h F*0% h % ."MFBPCR"\AS.seek 0.81ml F*5'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*_  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V @CEST1538776802.51 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *g'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*8 % +108 h % ."MFBPCR"\AS.seek 0ml F*]'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + &6 P%5 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538884969.89 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )h h  C' '/ h  P% %^ h  F* *C h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *s  h  .t\SPE.configure SPEconfig F*\ 2 *o% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'u& S)#.0X 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:44:06CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #13) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&T1 +150 h 1 M&B& h &_ M&Q% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &%(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &#'η +91 h 'η .tHand.close M&/ +150 h / M&y& h &- M&O% h %η .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +265 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &J' +91 h ' .t\CC.seek :closed C'(W +415 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C''& h &W C'K% h %4 .t\CSV.dial CSR C':  +151 h  C'Z% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '7'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '@% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'l'[ +463 h '[ .t\CSV.dial :waste C'{ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '4'4 +2010 h '4 #t\Purging external Intake C'>& h &W C'd% h %4 .t\CSV.dial :flush C'E  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +76 .t\CTV.dial :intake C'p  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'i'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'v& h &W C'I% h %4 .t\CSV.dial :flush C'f  +75 h  .t\CS.seek 5ml C'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C' % h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z +113 h .t\CTV.dial :air .\CS.seek 0ml C'0'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'.'0 +506 h '0 .t\CSV.dial :waste C'} +38 h .t\CS.seek 0ml C'N'4 +506 h '4 C'5& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +37 h @ C'% h %0 .t\CS.seek 5ml C'U'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'n'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.seek CC M&Y' +90 h ' .tHand.close M&F/ +150 h / .t\CC.seek :open C'( +416 h ( M&D& h &ӷ M&8% h % .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &,(- +265 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\\'η +91 h 'η .tHand.open M& 1 +150 h 1 M&w% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:44:11CEST30-Aug-18\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:44:06CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*[% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&h% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(/% h %X A(^. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (C(~ +6 F*s% h %&K ."readyMFB"\ATV.dial :waste2 F*o % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (u& h &~ M&2& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &R( +166 h ( M&& h & A(q& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &;'н +89 h 'н A((& h &~ .t\SE.seek 6.71 Pucks below Top Plate A(' +196 h ' A(& h &~ M&S& h & .t\Elbow.seek 3 M&a( +6 h ( M&"& h & A(3& h &~ .t\Forearm.seek 3 + 300 counts M&'- +23 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &?/ +150 h / ."downElevator"\SE.seek :down M&6& A('X h & M&-% h %- .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.seek CC M&e(ӷ +104 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' .t\CC.seek :closed C'W(W +416 h (W .tHand.open M&1 +150 h 1 M&t% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)$% h % C'+& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % .tIntake.select :close S)0 +7 )d% h % .t\SSV.dial :exhaust S)E  +75 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9ld +100 C'd9 h 9pd +100 C'E9 h 9td +100 C'79 h 9xd +100 C'9 h 9|d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9'd +100 C'n9 h 9>d +100 C'I9 h 9Td +100 C'p9 h 9kd +100 C'f9 h 9d +100 C'i9 h 9d +100 C' 9 h 9d +100 C'V9 h 9d +100 C'z9 h 9d +100 C'99 h 9d +100 C'09 h 9 d +100 C'49 h 9 d +100 C'v9 h 96d +100 C'.9 h 9Ld +100 C'59 h 9bd +100 C'9 h 9xd +100 C'|9 h 9d +100 C'N9 h 9d +100 C'_9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'U9 h 9Td +100 C'M9 h 9id +100 C'}9 h 9d +100 C'9 h 9d +100 C'*9 h 9d +100 C'F9 h 9d +100 C'G9 h 9d +100 C'9 h 9d +100 C'89 h 9  d +100 C'Y9 h 9 d +100 C'X9 h 9 *d +100 C'<9 h 9 ?d +100 C'&9 h 9 Td +100 C' 9 h 9 j d +100 C'D9 h 9 $d +100 C'k9 h 9 (d +100 C'g9 h 9 ,d +100 C'[9 h 9 0d +100 C'h9 h 9 4d +100 C'/9 h 9 8d +100 C'^9 h 9 ' +90 h ' A(d& h &  .t\SE.seek 12.71 Pucks below Top Plate A(E' +136 h ' M&7& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b .t\SE.seek 13.04 Pucks below Top Plate A(n'" +27 h '" M&I% h % A(p& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &f'; +21 h '; M&i& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &V' +22 h ' .t\SE.seek 14.04 Pucks below Top Plate A(z' +36 h ' M&9& h & M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +90 h '\  .t\Elbow.seek CC M&v(ӷ +142 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'N( +416 h ( A(_& h &  A(& h &  M& & h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&& h & A(U& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &M' +93 h ' .t\Elbow.hold M&}, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&*% h % A(F& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&G'; +21 h '; M&& h & A(8& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Y' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(<'X +100 (&% h %v M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'k @ +19 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'[ ` +18 h ` .t\AIV.dial :bypass C'h { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&^1 +2 h 1 M&s& h & M&o% h %\  .t\Elbow.seek FlushPuck::Garage M&w(- +166 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&q% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +265 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +90 h ' .t\CC.seek :closed C'](W +416 h (W C'& h &W .tHand.open M&?1 +150 h 1 M&u% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%3 +151 h P%6% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%R' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +38 h  .t\PS.seek 0ml P%-' +1597 h ' .t\PSV.dial PSR P%P +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'  h +75 .t\PSV.dial :mixloop P%a +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%L  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%$ +38 h .t\PS.seek 0ml P%+' +2610 h ' .t\PSV.dial :air P%t  +37 h  .t\PS.seek 2.5ml P%j' +2610 h ' .t\PSV.dial :mixloop P%Q +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&C& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +7 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&T/ +150 h / .t\CC.seek :open C'c( +416 h ( M&O& h &ӷ M&1% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +264 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &A'η +91 h 'η .tHand.open M&~1 +150 h 1 M&B% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&% h %\  A('% h %X A(=. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Z(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (\ & h &~ M&@& h &- M&K% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &{( +166 h ( M&l& h & A(d& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &e'н +90 h 'н A(7& h &~ .t\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' A(& h &~ M&I& h & .t\Elbow.seek 3 M&p( +6 h ( M&x& h & A(i& h &~ .t\Forearm.seek 3 + 300 counts M& '- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &9/ +150 h / ."downElevator"\SE.seek :down M&>& A(0'X h & M&:% h %- .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &f' +91 h ' .t\CC.seek :closed C'n(W +416 h (W .tHand.open M&V1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'4  +150 h  .t\CSV.dial CTV C'W  +19 h  .t\CS.seek 5ml C'_'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%U +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%v +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%*' +2610 h ' .t\CBV.dial :waste1 C'F @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'8 @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'X  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +18 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'&' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'k'4 +2647 h '4 .t\CBV.dial :waste1 C'[ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'h  +151 h C'/& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&^% h %\  A(s% h %X A(o. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (M( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (G& h &  M&z& h &ӷ M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &2( +142 h ( M&& h & A(<& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +90 h ' A(& h &  .t\SE.seek 13.71 Pucks below Top Plate A(}'n +125 h 'n M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &q' +25 h ' .t\Elbow.hold M&D, +7 h , .t\Elbow.reconfigure TightElbow A(\\- M&$&.,(\ #b h -+n .t\SE.seek 14.04 Pucks below Top Plate A(,' +27 h ' M&S% h % A(w& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&('; +21 h '; M&N& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &?' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(u' +36 h ' M&3& h & M&6% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +90 h '\  .t\Elbow.seek CC M&;(ӷ +142 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(a& h &  A(L& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &$( +143 h ( M&& h & A(t& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &+' +92 h ' .t\Elbow.hold M&Q, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &j1 +150 h 1 M&C% h % A(!& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &`'; +21 h '; M&T& h & A()& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &m' +22 h ' A(]% h % ."downElevator"\SE.seek :down A(O'X +100 (1% h %- M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast .\PSV.dial :air A(6 P%%  +113 h  P%r% h % .t\PS.seek 0.499ml P%~' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%B +38 h .t\PRV.dial :air P%b  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%'  +18 h  .t\PRV.dial :air P%=  +19 h  P%\ % h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %@' +251 h ' .t\PRV.dial :waste2 P%K @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %A' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'l& h & .tHand.open M&d1 +2 h 1 M&7& h & M&y% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&x'η +91 h 'η .tHand.close M&i/ +150 h / M&9& h &- M&>% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +264 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'& h &W .tHand.open M&p1 +150 h 1 M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  C'c& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%V @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%4  +76 h  .tdelay 2 seconds +200 P%W% h % .t\PS.seek 1.499ml P%f' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%Z' +1094 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%_' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %U'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%v% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%F'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%8  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %X' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%&' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%[ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%h' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%/  +75 h  .t\PS.seek 2.5ml P%^' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%s +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%o  +75 h P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %z' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'Y & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %<'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1312 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%\\ +37 h .t\PS.seek 0ml P%S' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'w& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'N& h &W ."MFBPCR"\ASV.dial :SPEdelivery F* @@T .t\AWV.select :air F*|  .t\AIV.dial :bypass .\PSV.dial PSR P%3 +22 h  ."MFBPCR"delay 20 seconds +91 h P%6% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*2 I +129 h I F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *q'6 +66 .t\PSV.dial :waste1 P%R  +38 h  .t\PS.seek 0ml P%P' +1597 h ' .t\PSV.dial PSR P%( +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%,' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%" +21 h  F*n% h %6 ."MFBPCR"\AS.seek 0.329ml F*a'ͯ +92 h P%L% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%t  +37 h  .t\PS.seek 0ml P%-' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*;  +21 h  F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *C' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%! +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%T' +217 ."MFBPCR"\ATV.dial :AIV F*) I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%$ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%1' +2610 h ' .t\PSV.dial PSR P% +113 h P%+% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%`' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*r9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*m n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*O% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%B' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%b +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%'% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%=' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\  +37 h  .t\PS.seek 0ml P%~' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%K +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%@' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %7'k +2679 h 'k .t\PSV.dial :waste1 P%y  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %I' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *Q'V +849 h ' .t\PSV.dial :air P%i  +113 h  .t\PS.seek 2.5ml P%9' +2610 h ' .t\PSV.dial :mixloop P%> +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %H' +5106 h ' .t\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %e' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*.  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *:'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*p' +170 h ' .t\PSV.dial :mixloop P%0 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %c' +619 h ' ."MFBPCR"\ATV.dial :waste F*V n +128 h n ."MFBPCR"\AS.seek :empty F*5'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*4% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *W' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*A  +43 h F*f% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'Z  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '_  +38 h  +113  .t\CBV.dial :waste1 C'v @ +38 h @ .t\CS.seek 0ml C'U'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'8  +37 h  C'F% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*X% h %ݏ ."MFBPCR"\AS.seek 0.419ml F* '& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*[% h %& ."MFBPCR"\AS.seek 0.092ml F*&'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'/' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C's'4 +2647 h '4 C'o& h &W #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +7 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&z' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'<( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &|'η +91 h 'η .tHand.open M&31 +150 h 1 M&6% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'2& h & M&}% h %\  A(R% h %X A(#. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (J(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &~ M&P& h &- M&?% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &,( +166 h ( M&& h & A(n& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &"'н +89 h 'н A(L& h &~ ."WCR"\SE.seek 4.71 Pucks below Top Plate A(q' +215 h ' A(D& h &~ M&& h & ."WCR"\Elbow.seek 3 M&a( +6 h ( M&t& h & A(;& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&j'- +23 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +40 h '&0 ."MFBPCR"delay 3 seconds +110 h / ."downElevator"\SE.seek :down M&!& A('X h & M&)% h %- ."WCR"\Forearm.seek :retract M&]'\ +90 h '\  ."WCR"\Elbow.seek CC M&-(ӷ +100 ."MFBPCR"\ATV.dial :waste2 F*C % +24 h 'X ."downElevator"\SE.coast A(5 +18 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R + h (ӷ M&T& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' ."WCR"\CC.seek :closed C'1(W +416 h (W ."WCR"Hand.open M&u1 +52 h 'R ."MFBPCR"\ATV.dial :air2 F*`  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *r' +76 h 1 M&m% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)*% h % C'%& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'b  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X'\  +1032 h '\  F*7% h %\  ."MFBPCR"\AS.seek 0.5ml F*H'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*.% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*Q % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +3472 h 'V ."MFBPCR"\SPE.coast F*x  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0709h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*0  +10 h  F*@% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*p n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +22 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *:'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*W  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*k' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*v % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*_'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*F'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*h% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*G % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254679 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'Y  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'o @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'{ @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'E ` +19 h ` ."WCR"\CS.seek 7.288ml C'z' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'w  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '^'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'3  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'N' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'\\  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'6  +38 h  C'}% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'#' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'2 @ +76 h @ +50 S)R% h % ."WCR"\CBV.dial between CSV and waste2b C'?  +94 h  C'|% h % ."WCR"\CSV.dial between air and CTV C'P ` +56 h ` ."WCR"\CS.seek 7.288ml C'n' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'L  ."WCR"\CSV.dial CTV C'  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'S'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'D  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C't  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C';  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C', @ +75 h @ +50 S)"% h % ."WCR"\CBV.dial between CSV and waste2b C')  +94 h  C'T% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'C' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'q  'a  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'j  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ']' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'<  +151 h C'&& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&u% h %\  A(m% h %X A(`. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ($& h &  M&*& h &ӷ M&+% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &J( +143 h ( M&b& h & A('& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  ."WCR"\SE.seek 14.71 Pucks below Top Plate A(\ '] +116 h '] M&& h & A(=& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &M' +25 h ' ."WCR"\Elbow.hold M&~, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(d- M&$&.,(\ #b h -+] ."WCR"\SE.seek 15.04 Pucks below Top Plate A(y' +27 h ' M&K% h % A(r& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&l'; +21 h '; M&& h & A(I& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &i' +22 h ' ."WCR"\SE.seek 16.04 Pucks below Top Plate A(7' +37 h ' M&>& h & M&.% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  ."WCR"\Elbow.seek CC M&Q(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&0/ +150 h / ."WCR"\CC.seek :open C'@( +416 h ( A(9& h &  A(e& h &  M&p& h &ӷ M&V% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&A& h & A(f& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &4' +92 h ' ."WCR"\Elbow.hold M&W, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&:% h % A( & h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'; +21 h '; M&Z& h & A(v& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(k% h % ."downElevator"\SE.seek :down A('X +100 (8% h %X M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'U& h & .tHand.open M&F1 +2 h 1 M&X& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&['η +91 h 'η .tHand.close M&h/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +265 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &{' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'w& h &W C'% h % .t\CSV.dial CSR C'z  +37 h  C'3% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'^'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '\\% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '6'[ +463 h '[ .t\CSV.dial :waste C'} +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'N'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'#& h &W C'2% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '?'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'P% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'L  +37 h  +113  .t\CBV.dial :waste1 C'n @ +76 h @ .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'S& h &W C't% h %4 .t\CSV.dial :flush C';  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C')  +76 h  .t\CS.seek 0ml C'T'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'!% h %4 .t\CSV.dial :flush C'a  +75 h  .t\CS.seek 5ml C'q'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +38 h @ C'j% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'< .t\CBV.dial CSV C'&  +37 h +76 .t\CTV.dial :air C'u  +150 h .t\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'`'0 +507 h '0 .t\CSV.dial :waste C'$ +37 h .t\CS.seek 0ml C'*'4 +507 h '4 C'+& h &W C'b% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'% h %0 .t\CS.seek 5ml C'='p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'd'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C'r +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'I& h &W C'O% h %4 .t\CSV.dial CSR C'>  +150 h  C'.% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'J' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +113 h .t\CTV.dial :intake C'M  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'x  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'y  +113 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  '1  +37 h  +113  .t\CBV.dial :waste1 C'9 @ +38 h @ .t\CS.seek 0ml C'e'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'p  +37 h  C'V% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'A'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'Q  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C':  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k  ' +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'8'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'U +38 h .t\CS.seek 0ml C'F'4 +506 h '4 #t\Flushing upper manifold C'X& h &W C'% h %4 .t\CSV.dial :flush C'4  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'%'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'h'4 +506 h '4 C' & h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'['0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C's% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'G'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'w% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +38 h @ C'{% h %p .t\CS.seek 7ml C']'i +415 h 'i ."connect"\CSV.dial CTV C'\\  .t\CTV.dial CSV C'^  +38 h  +113  .t\CBV.dial :waste1 C'} @ +38 h @ .t\CS.seek 0ml C'6'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'#& h &W C'2% h %4 .t\CSV.dial :flush C'R  +75 h  .t\CS.seek 5ml C'N'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'P% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'L  +76 h  .t\CS.seek 0ml C'n'4 +766 h '4 #tDisinfecting external Intake with kill C'(& h &W C'S% h %4 .t\CSV.dial CSR C't  +37 h  C';% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'D'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'"  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C')' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C',  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'!  +113 h  C'a% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'T'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'C' +1373 h ' .t\CSV.dial CBV C'j +113 h .t\CS.seek 0ml C'q'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +6 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&m' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'`( +416 h ( M&+& h &ӷ M&b% h % .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &*(- +265 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&=1 +150 h 1 M&d% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'K& h & .tHand.open M&I1 +2 h 1 M&O& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &>(- +7 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&\ / +150 h / M&M& h &- M&J% h %η .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'~(W +416 h (W C'H& h &W .tHand.open M&11 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'p& h &W C'V% h %4 .t\CSV.dial CSR C'e  +113 h  C'A% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'Q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C': +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Purging external Intake C'& h &W C'Z% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'v  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'8'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'U  +76 h #t\Flushing upper manifold C'_& h &W C'X% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'4'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C'7% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'W'4 +506 h '4 C'%& h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'o% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'['4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'G +75 h .t\CS.seek 0ml C' '4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&@& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +7 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&3/ +150 h / .t\CC.seek :open C'{( +416 h ( M&z& h &ӷ M&\\% h % .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &}(- +265 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &2'η +91 h 'η .tHand.open M&R1 +150 h 1 M&6% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Completed [mbaPhase] begun 06:03:05 #\Preparing to sleep until Tue Oct 09 06:03:05 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:03CEST09-Oct-18 #\Continuing at 06:03CEST09-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeploy.rb:120\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:45:37CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:03CEST09-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0709h25ml.pcr F*P% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*L % +86 h % F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +64 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*$ % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*+% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *K'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*I' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*` n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*9  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +21 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *e'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*A  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*Q  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*:' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*f  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*k  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*7% h % \\ ."MFBPCR"\AS.seek 0.326ml F*4'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0711h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +21 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*#  +31 h  ."MFBPCR"\ATV.dial :air3 F*E % +87 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*S  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*a  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*T'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*C n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*$  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*+ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*- n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*=  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*''< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*K% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*O n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*J  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F**  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*y'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*r % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0713h25ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*x  +22 h ."MFBPCR"\ATV.dial :air3 F*H % +86 h % F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *p'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*A'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*q% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +64 h  F*k% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*U  +32 h  ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*%% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*@  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*0'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*z n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\\'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*#  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*^ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*6' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*P I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*L % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*( n +21 h n ."MFBPCR"delay 2 seconds +200 F*n% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*,  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*)% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0716h25ml.pcr F*!% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*C  +21 h ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *$'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*b% h %n ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*<% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*J% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*H% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*k  +43 h  ."MFBPCR"\ARV.dial :carrier F*f  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*Z'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*7' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*B  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.536ml F*4'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +21 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*@  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0718h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*z  +22 h ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*R% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*]'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +21 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *S'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *,' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*j% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*T  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*O n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*r % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*i' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*`  +22 h  ."MFBPCR"delay 7 seconds +700 F*9% h %< ."MFBPCR"\AS.seek 0.594ml F*V'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*1 n +21 h n ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*k'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*: @@T *Z  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*v  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *U' +424 h ' ."MFBPCR"\ATV.dial :ASV F*8 n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*F  +43 h  F*_% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *B'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*7 % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *%' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*4% h % ."MFBPCR"\AS.seek 0.71ml F*h' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*W% h % H ."MFBPCR"\AS.seek 0.735ml F*s' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*g' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*[ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*w% h % zG ."MFBPCR"\AS.seek 0.365ml F*G' +7161 h ' ."MFBPCR"\ATV.dial :waste F*@ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*3% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.579ml F*0' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %  ."MFBPCR"\AS.seek 0.604ml F*#'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*2% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*P n +86 h n ."MFBPCR"\ASV.dial :wasteR F*/  +22 h  ."MFBPCR"\AS.seek 0ml F*]'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *?'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*6% h %V ."MFBPCR"\AS.seek 0.01ml F*S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*(' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*;' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*) n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*"  +21 h  ."MFBPCR"\AS.seek 0ml F*,'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *!'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *C' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*j n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*T n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +202 h 'R ."MFBPCR"\SPE.coast F*Y  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*u'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*$ n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*+  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*- I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *b'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*=  +43 h  F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *m': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*d  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *K'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*O n +65 h n F*'% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *.' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*\ I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*I  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*r% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *>'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F** n +65 h n F*x% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *H' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*i I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*9  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *y': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*1 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *p'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*V n +150 h n ."MFBPCR"\AS.seek 0ml F*e'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*A  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*l % +43 h % F*q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*f'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %0 ."MFBPCR"\AS.seek 0.985ml F*k' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*8 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *X'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*B  +43 h  F*7% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9987 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*%' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*4'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*W  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +43 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*[% h %Z ."MFBPCR"\AS.seek 0.81ml F*w'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*G'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*~  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +65 h  F*z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *E'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*/  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*6% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V @CEST1538949617.95 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*& % +108 h % ."MFBPCR"\AS.seek 0ml F*$'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast F*5 C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast P%6 M&5 +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539057785.33 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )+ h  C' ' h  P% %u h  F* *- h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *b% h %V .t\AS.configure ASconfig S)=% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %i' +20 h '\  ."closeHand"Hand.close M&H/ .t\Elbow.jog 1500 M&9* +17 h ."readyCS"\CS.reconfigure CSfast .\CS.home.seek 0ml ."rdySampler"\SS.reconfigure SSconfig C'#d @b '`'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:46:34CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #14) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&k1 +150 h 1 M&8& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &F(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&B& h &- M&7% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'%& h &W .tHand.open M&41 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'o& h &W C'% h %4 .t\CSV.dial CSR C's  +150 h  C'[% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '@'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'3& h &W C'G% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z '  h +75 .t\CTV.dial :intake C'E  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'R'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'0  +75 h #t\Flushing upper manifold C'^& h &W C'2% h %4 .t\CSV.dial :flush C'#  +75 h  .t\CS.seek 5ml C'/'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C']% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'6'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +507 h '0 .t\CSV.dial :waste C'N +37 h .t\CS.seek 0ml C''4 +507 h '4 C'?& h &W C'n% h %4 .t\CSV.dial :flush C't  +37 h  .t\CS.seek 3ml C'S'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +38 h @ C'(% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C')'0 +507 h '0 .t\CSV.dial :waste C'" +75 h .t\CS.seek 0ml C';'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M&C/ +150 h / .t\CC.seek :open C'Y( +416 h ( M&T& h &ӷ M&&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &c(- +264 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M& 1 +150 h 1 M&5% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:46:38CEST30-Aug-18\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:46:34CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*{% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C':& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&+% h %\  A(% h %X A(u. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (-(~ +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*b % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *=' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (<& h &~ M&& h &- M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&m& h & A(O& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &''н +90 h 'н A(K& h &~ .t\SE.seek 3.71 Pucks below Top Plate A(\ ' +223 h ' A(.& h &~ M&& h & .t\Elbow.seek 3 M&I( +7 h ( M&& h & A(& h &~ .t\Forearm.seek 3 + 300 counts M&J'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down A(x'X M&r& h & M&>% h %- .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.seek CC M&V(ӷ +133 h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +90 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&Z1 +150 h 1 M&M% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'l& h &W ."airStroke"\CSV.dial :waste S)e% ."airStroke"\CS.seek 0ml h % C''4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9zd +100 C'9 h 9d +100 C''9 h 9d +100 C'99 h 9 d +100 C'9 h 9$d +100 C'J9 h 9(d +100 C'\ 9 h 9 ,d +100 C'I9 h 9$0d +100 C'x9 h 9<4d +100 C'H9 h 9S8d +100 C'Y9 h 9kd +100 C'F9 h 9Td +100 C'X9 h 9kd +100 C'v9 h 9d +100 C'%9 h 9d +100 C'49 h 9d +100 C'79 h 9d +100 C'9 h 9d +100 C'o9 h 9d +100 C'9 h 9 d +100 C's9 h 9 d +100 C'9 h 96d +100 C' 9 h 9Ld +100 C'B9 h 9bd +100 C'g9 h 9xd +100 C'9 h 9d +100 C'w9 h 9d +100 C'[9 h 9d +100 C'@9 h 9d +100 C'G9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'39 h 9(d +100 C'E9 h 9>d +100 C'W9 h 9Td +100 C'z9 h 9id +100 C'9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9d +100 C'29 h 9d +100 C'R9 h 9d +100 C']9 h 9d +100 C'09 h 9  d +100 C'9 h 9 d +100 C'/9 h 9 *d +100 C'P9 h 9 ?d +100 C'#9 h 9 Td +100 C'9 h 9 j d +100 C'N9 h 9 $d +100 C'L9 h 9 (d +100 C'?9 h 9 ,d +100 C'n9 h 9 0d +100 C'9 h 9 4d +100 C'D9 h 9 8d +100 C'(9 h 9 '4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +112 h .t\CBV.dial CSV C''  +19 h C'9% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'J  +38 h C'\ % h %p .t\CS.seek 7ml C'I'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'x @ +37 h @ .t\CS.seek 10ml C'H' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'Y ` +132 h ` #tLysing Complete C'Z& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&M% h %\  A(l% h %X A(e. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (8( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (Q& h &  M&;& h &ӷ M&-% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &1( +143 h ( M&_& h & A(i& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(U& h &  .t\SE.seek 15.71 Pucks below Top Plate A(q'L +107 h 'L M&& h & A(=& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &k' +25 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+L .t\SE.seek 16.04 Pucks below Top Plate A(' +27 h ' M&F% h % A(X& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&v'; +20 h '; M&%& h & A(4& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &7' +23 h ' .t\SE.seek 17.04 Pucks below Top Plate A(' +36 h ' M&o& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'w( +416 h ( A([& h &  A(@& h &  M&G& h &ӷ M&~% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &3( +142 h ( M&E& h & A(W& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &z' +93 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\\1 +150 h 1 M&^% h % A(2& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &R'; +20 h '; M&]& h & A(0& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +23 h ' A(/% h % ."downElevator"\SE.seek :down A(P'X +92 h 'X ."downElevator"\SE.coast A(5 +8 (#% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +89 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'L @ +19 h @ .t\CS.seek 10ml C'?' +6 h ' .t\CSV.dial between air and CTV C'n ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&(1 +2 h 1 M&S& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&6(- +166 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &- .t\Forearm.seek :garage M&,'η +91 h 'η .tHand.close M&t/ +150 h / M&!& h &- M&C% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +264 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +91 h ' .t\CC.seek :closed C'}(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%j +151 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%{  +38 h  .t\PS.seek 0ml P%5' +1597 h ' .t\PSV.dial PSR P%+ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%:' +2610 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'u  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%<  h .t\PS.seek 0ml P%b' +2610 h ' .t\PSV.dial :air P%d  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%m' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%K' +2610 h ' .t\PSV.dial :mixloop P%. +37 h .t\PS.seek 0ml P%$' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&*' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C'( +415 h ( M&& h &ӷ M&'% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +265 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&x1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'Y& h & M&Z% h %\  A(M% h %X A(l. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (e(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (Q& h &~ M&;& h &- M&-% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &_( +166 h ( M&i& h & A(U& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &V'н +89 h 'н A(& h &~ .t\SE.seek 2.71 Pucks below Top Plate A(=') +235 h ') A(& h &~ M&F& h & .t\Elbow.seek 3 M&X( +7 h ( M&%& h & A(4& h &~ .t\Forearm.seek 3 + 300 counts M&y'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &o/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&1% h %- .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.seek CC M&k(ӷ +142 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' + h 'X ."downElevator"\SE.coast A(5 +90 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&71 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C's  +151 h  .t\CSV.dial CTV C'  +18 h  .t\CS.seek 5ml C'['p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'@' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'G'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%E' +2609 h ' .t\PSV.dial :mixloop P%W +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%\\  +151 h  .t\PS.seek :empty P%^' +2609 h ' .t\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C']  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'0 @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'/  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'3 @ +38 h @ .t\CS.seek 10ml C'#' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'L'4 +2648 h '4 .t\CBV.dial :waste1 C'n @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'D& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&(% h %\  A(S% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (z( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (R& h &  M&& h &ӷ M&P% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&"& h & A()& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +89 h ' A(H& h &  .t\SE.seek 16.71 Pucks below Top Plate A('; +96 h '; M&t& h & A(!& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &C' +25 h ' .t\Elbow.hold M&N, +7 h , .t\Elbow.reconfigure TightElbow A(,- M&$&.,(\ #b h -+; .t\SE.seek 17.04 Pucks below Top Plate A(h' +27 h ' M&T% h % A(6& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + & '; +21 h '; M&w& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 18.04 Pucks below Top Plate A(' +36 h ' M&j& h & M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +90 h '\  .t\Elbow.seek CC M&{(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&5' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'u( +416 h ( A(& h &  A(<& h &  M&:& h &ӷ M&d% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &A( +142 h ( M&& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &m' +93 h ' .t\Elbow.hold M&., +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &K1 +150 h 1 M&c% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &p'; +21 h '; M&r& h & A(a& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &|' +22 h ' A(}% h % ."downElevator"\SE.seek :down A('X +83 h 'X ."downElevator"\SE.coast A(5 +17 ('% h %X M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +89 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%\  +113 h  P%O% h % .t\PS.seek 0.499ml P%x' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%9  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%Y  +38 h .t\CBV.dial AIV C'Z  +37 h .t\PRV.dial between CBV and air P%M  +19 h  .t\PRV.dial :air P%l  +19 h  P%Q% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %;' +250 h ' .t\PRV.dial :waste2 P%- @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&U1 +2 h 1 M&& h & M&>% h %\  .t\Elbow.seek FlushPuck::Garage M&I(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&4/ +150 h / M&o& h &- M&% h %η .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +265 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' +91 h ' .t\CC.seek :closed C'y(W +416 h (W C'=& h &W .tHand.open M&X1 +150 h 1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%7 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%s  +75 h  .tdelay 2 seconds +200 P% % h % .t\PS.seek 1.499ml P%v' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%e' +1094 h ' .t\PSV.dial :air P%?  +37 h  .t\PS.seek 0ml P%[' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%@ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%~  +37 h  .tdelay 2 seconds +200 P%$% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %W'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%g  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%\\' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%E  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%]  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%0  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %/' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck P%3 @ +75 h @ .t\PS.seek 0ml P%G' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%#' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%n +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%D  +75 h  .t\PS.seek 2.5ml P%(' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%S +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%R% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'P & +50 h & .t\AWV.select :waste C' +6 P%"% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'H { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %k' +1313 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 2.5ml P%!' +2609 h ' .t\PSV.dial :PML P%, +38 h .t\PS.seek 0ml P%T' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '6& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'w& h &W F* @@T ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass F*  .t\PSV.dial PSR P%j +22 h  ."MFBPCR"delay 20 seconds +91 h P% % h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%8' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *C'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%+' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%h' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%N' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%& +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%:' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*d % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%5' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*{  +22 h  F*K% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *c' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%r' +217 ."MFBPCR"\ATV.dial :AIV F*a I +43 h I F*}% h % ."MFBPCR"\AS.seek 0.469ml F*b' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%'' +2609 h ' .t\PSV.dial PSR P%* +113 h P%m% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%\ ' +739 h ' ."MFBPCR"delay 5 seconds +500 F*O9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*|% h % ."MFBPCR"\AS.seek 0.041ml F*z'% +336 h ' .t\PSV.dial PSR P%^ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%9 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Y' +2610 h ' .t\PSV.dial PSR P%Z +113 h P%M% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%l' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%Q  +37 h  .t\PS.seek 0ml P%x' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%- +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%>  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*F I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *.'V +849 h ' .t\PSV.dial :air P%4  +113 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %J' +5107 h ' .t\PSV.dial :air P%%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %V' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *1'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*u I +43 h I F*=% h %Q ."MFBPCR"\AS.seek 0.01ml F*X' +171 h ' .t\PSV.dial :mixloop P%q +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*7 n +129 h n ."MFBPCR"\AS.seek :empty F*B'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*s% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*v% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *?'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'e  +38 h  C'@% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'$' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'[  'g  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'W'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*E  +22 h ."MFBPCR"delay 3 seconds +300 F*\\% h %_ ."MFBPCR"\AS.seek 0.434ml F*]'ݏ +109 h '4 .t\CSV.dial CSR C'0  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*3  +22 h F*/% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*G n +108 h n F*n% h %& ."MFBPCR"\AS.seek 0.092ml F*#'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'D' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'S'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +6 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&H/ +150 h / .t\CC.seek :open C')( +416 h ( M&t& h &ӷ M&k% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &!(- +265 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&j1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'& h & M&% h %\  A(% h %X A(`. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (8(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ( & h &~ M&+& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &h( +166 h ( M&& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &&'н +89 h 'н A(<& h &~ ."WCR"\SE.seek 1.71 Pucks below Top Plate A(C': +244 h ': A(N& h &~ M&d& h & ."WCR"\Elbow.seek 3 M&( +7 h ( M& & h & A({& h &~ ."WCR"\Forearm.seek 3 + 300 counts M&K'- +22 h '- ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &:/ +10 h '&0 ."MFBPCR"delay 3 seconds +140 h / ."downElevator"\SE.seek :down M&& A('X h & M&a% h %- ."WCR"\Forearm.seek :retract M&}'\ +91 h '\  ."WCR"\Elbow.seek CC M&5(ӷ +69 ."MFBPCR"\ATV.dial :waste2 F*c % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +30 h (ӷ M&r& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' ."WCR"\CC.seek :closed C''(W +416 h (W ."WCR"Hand.open M&1 +23 h 'R ."MFBPCR"\ATV.dial :air2 F*p  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *O' +105 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)b+ +7 h + S)^% h % C'\ & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'9  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X  +43 h  #"MFBPCR"\Swish #1 of 2 F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *F'\  +1032 h '\  F*i% h %\  ."MFBPCR"\AS.seek 0.5ml F*4'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*J'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F* % h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *I' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*. % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +3473 h 'V ."MFBPCR"\SPE.coast F*%  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0907h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*=  +64 h  ."MFBPCR"\ARV.dial :carrier F*q  +10 h  F*;% h %V ."MFBPCR"\AS.seek 0.5ml F*V'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *1'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*e' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*~  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.53ml F*L' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\\  +107 h  ."MFBPCR"delay 7 seconds +700 F*0% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +21 h n ."MFBPCR"delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.6ml F*?'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*n  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*R % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254709 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'P  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'_ @ +76 h @ +50 S)"% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'6  ."WCR"\CSV.dial CTV C'k  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '('4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'j  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'w' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C',  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'`' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'+ ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'<  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'T'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'N  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '!' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'{  +38 h  C'd% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C':' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'h @ +75 h @ +50 S)&% h % ."WCR"\CBV.dial between CSV and waste2b C'a  +94 h  C'r% h % ."WCR"\CSV.dial between air and CTV C'* ` +57 h ` ."WCR"\CS.seek 7.288ml C'c' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'K  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '#'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'C  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C')  +150 h C'}& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&% h %\  A(% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (\ ( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (A& h &  M&^& h &ӷ M&m% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &8( +142 h ( M&9& h & A(M& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(b& h &  ."WCR"\SE.seek 17.71 Pucks below Top Plate A(Q'* +86 h '* M&Y& h & A(l& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &z' +25 h ' ."WCR"\Elbow.hold M&x, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(U- M&$&.,(\ #b h -+* ."WCR"\SE.seek 18.04 Pucks below Top Plate A(>' +27 h ' M&-% h % A(O& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &i'; +21 h '; M&F& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &4' +22 h ' ."WCR"\SE.seek 19.04 Pucks below Top Plate A(' +36 h ' M&& h & M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +90 h '\  ."WCR"\Elbow.seek CC M&.(ӷ +142 h (ӷ M&I& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ ."WCR"\Forearm.seek CC M&=' +91 h ' ."WCR"Hand.close M&q/ +150 h / ."WCR"\CC.seek :open C';( +416 h ( A(o& h &  A(V& h &  M&X& h &ӷ M&7% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &u( +142 h ( M&& h & A(v& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &s' +93 h ' ."WCR"\Elbow.hold M& , +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &@1 +150 h 1 M&1% h % A(~& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&['; +21 h '; M&e& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +22 h ' A(L% h % ."downElevator"\SE.seek :down A(\\'X +74 h 'X ."downElevator"\SE.coast A(5 +26 (0% h %X M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&21 +2 h 1 M&/& h & M&3% h %\  .t\Elbow.seek FlushPuck::Garage M&?(- +166 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&/ +150 h / M&G& h &- M&% h %η .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &_' +91 h ' .t\CC.seek :closed C'"(W +416 h (W C'y& h &W .tHand.open M&t1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'k& h &W C'6% h % .t\CSV.dial CSR C'  +38 h  C'j% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'('p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 ',% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'w'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'`& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'+% h %p .t\CS.seek 7ml C' 'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '<  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'N'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'T& h &W C' % h %4 .t\CSV.dial :flush C'{  +76 h  .t\CS.seek 5ml C'd'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C':% h %p .t\CS.seek 7ml C'h'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  +38 h  .t\CBV.dial :waste2b C'a  +75 h  .t\CS.seek 0ml C'r'4 +766 h '4 #t\Flushing upper manifold C'*& h &W C'% h %4 .t\CSV.dial :flush C'K  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +38 h @ C'C% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C') '}  +37 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'^'4 +507 h '4 C'm& h &W C'9% h %4 .t\CSV.dial :flush C'M  +37 h  .t\CS.seek 3ml C'b'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C'Y% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'U'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +506 h '0 .t\CSV.dial :waste C'O +76 h .t\CS.seek 0ml C'F'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'|% h %4 .t\CSV.dial CSR C'  +151 h  C' % h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'Z'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'8' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q +113 h .t\CTV.dial :intake C'z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'I' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'%  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'>  +113 h  C'4% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'x'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'J  ''  +38 h  +113  .t\CBV.dial :waste1 C'o @ +38 h @ .t\CS.seek 0ml C'V'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'X  +38 h  C'7% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +709 h ' ."connect"\CSV.dial CTV C'=  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'.  +75 h  .t\CS.seek 0ml C'@'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'1  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C'L .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'u  +151 h .t\CS.seek 0ml C'0'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'W +37 h .t\CS.seek 0ml C'2'4 +507 h '4 #t\Flushing upper manifold C'/& h &W C'3% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C']% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ +112 h .t\CTV.dial :air .\CS.seek 0ml C'$'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'G& h &W C'% h %4 .t\CSV.dial :flush C'E  +38 h  .t\CS.seek 3ml C'n'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'S% h %0 .t\CS.seek 5ml C'?'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'R'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C';'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C't'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'k% h %4 .t\CSV.dial :flush C'6  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +37 h @ C'_% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C',  .t\CTV.dial CSV C'(  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'`& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'+% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'<  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tDisinfecting external Intake with kill C' & h &W C'T% h %4 .t\CSV.dial CSR C'  +38 h  C'{% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'N'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C': 'd  +37 h +76 .t\CTV.dial :intake C'&  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'a' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'h  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '*'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'K% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'r'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'c' +1372 h ' .t\CSV.dial CBV C'C +113 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&}& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&A/ +150 h / .t\CC.seek :open C'p( +416 h ( M&m& h &ӷ M&9% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &^(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +91 h 'η .tHand.open M&l1 +150 h 1 M&U% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'-& h & .tHand.open M&1 +2 h 1 M&|& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.seek :garage M&Z'η +91 h 'η .tHand.close M&Q/ +150 h / M&z& h &- M&8% h %η .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +264 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &i' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'J& h &W .tHand.open M&'1 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'X& h &W C'7% h %4 .t\CSV.dial CSR C'V  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'='p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '.% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'v'[ +462 h '[ .t\CSV.dial :waste C'1 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +2010 h '4 #t\Purging external Intake C'@& h &W C'e% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'0'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'W  +75 h #t\Flushing upper manifold C'g& h &W C'/% h %4 .t\CSV.dial :flush C'3  +76 h  .t\CS.seek 5ml C's'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'[ +112 h .t\CTV.dial :air .\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'$ +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'\ & h &W C'G% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'E'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'S'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'n'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C'R +75 h .t\CS.seek 0ml C'y'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'_( +416 h ( M&& h &ӷ M&,% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [coreNEG] begun 06:03:20 #\Preparing to sleep until Thu Oct 11 06:03:20 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:03CEST11-Oct-18 #\Continuing at 06:03CEST11-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:48:03CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:03CEST11-Oct-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0907h.pcr F*+% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*{'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*d'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*!'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*a  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*#  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*l% h %m ."MFBPCR"\AS.seek 0.82ml F*U'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*M  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0909h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +10 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*p n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*X  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *V'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*.  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*1' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*v  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*0  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*s'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0909h.pcr F*\\% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*n'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*6  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *I'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*T  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0912h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*!  +43 h  ."MFBPCR"\ARV.dial :carrier F*K  +11 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*P  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *"'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*5 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*)' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*l  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*U  +108 h  ."MFBPCR"delay 7 seconds +700 F*-% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*| n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*F'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*b  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*^  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*>'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*O % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0912h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*%  +21 h ."MFBPCR"\ATV.dial :air3 F*J % +87 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *X'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*'% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*v'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*W  +32 h  ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\ % h %m ."MFBPCR"\AS.seek 0.82ml F*G'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *6'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0914h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*;  +10 h  F*I% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *,'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*+ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*< % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*N'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*{  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*d  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*a% h % \\ ."MFBPCR"\AS.seek 0.326ml F*!'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0914h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*c  +22 h ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*#'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +21 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*9% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*l  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Y  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +31 h  ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*z% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct0916h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*v  +10 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*W  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*0  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b */'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*3 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*s'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +22 h  ."MFBPCR"delay 7 seconds +700 F*G% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*S  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*n  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct0916h.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *`'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*<  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *T'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +21 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*K'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*!'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*C% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*r  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0919h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*)  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +21 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*Z I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*^' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*O % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*%  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.536ml F*>'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*p  +22 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*7'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*' n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*i'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*.  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0919h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*1 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*e  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *W' +424 h ' ."MFBPCR"\ATV.dial :ASV F*0 n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*2  +43 h  F*g% h % ."MFBPCR"\AS.seek 0.62ml F*3'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*[ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b */'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*\\'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\ ' +424 h ' ."MFBPCR"\ATV.dial :air3 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*s% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*S' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*?' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*n n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*k% h % zG ."MFBPCR"\AS.seek 0.365ml F*R' +7161 h ' ."MFBPCR"\ATV.dial :waste F*; n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*,% h % ."MFBPCR"\AS.seek 0.579ml F*q' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*`'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*t' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*+ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*D  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F* % h %V ."MFBPCR"\AS.seek 0.01ml F*T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F* ' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h % q ."MFBPCR"\AS.seek 0.605ml F*:'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*d% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*{' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*a n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*&  +21 h  ."MFBPCR"\AS.seek 0ml F*h'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*K  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *c' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*C n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *!'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*r n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +202 h 'R ."MFBPCR"\SPE.coast F*P  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*A n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*m  +43 h  F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *#' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*5 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*l  +43 h  F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*U  +64 h  F*Y% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *-'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*| n +65 h n F*M% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *z': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*b  +64 h  F*O% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*^ n +65 h n F*%% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *J' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*4 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*o  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *>': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*' n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *X'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*7 n +150 h n ."MFBPCR"\AS.seek 0ml F*V'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*i % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*=% h % ."MFBPCR"\AS.seek 0.96ml F*v'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*u% h % ."MFBPCR"\AS.seek 0.97ml F*L'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*0 I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *g'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *['R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b */'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *\\' +9988 h ' ."MFBPCR"\ATV.dial AIV F*] I +43 h I ."MFBPCR"\AS.seek 0.02ml F*\ ' +9880 h ' ."MFBPCR"\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.seek 0ml F*s'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*$% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +21 h F*n% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*x  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*t % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *!'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*} % +108 h % ."MFBPCR"\AS.seek 0ml F*A'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539230600.77 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )m h  C' 'H h  P% % h  F* *5 h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *#  h  .t\SPE.configure SPEconfig F*\ 2 *9% h %V .t\AS.configure ASconfig S)l% F*#,,'T(^20( b h % .t\SS.configure SSconfig C')& S)#.0X(d +47 h (d +59 / A(7& h &,? .t\SE.reconfigure SEconfig .\SE.home.seek :down A(#K-0-\ b (X'X +148 h 'X .t\SC.reconfigure SCsloppy .\SC.home.seek :initial A($-.(\ 1@~b (( +346 h ' +13 (  .t\Forearm.seek :retract M&i'\ +6 h '\  .t\Elbow.seek :hammer M&(_ + h '4 +92 (_ .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &V' +91 h ' M&@% h % .t\Forearm.seek :retract M&='\ +90 h '\  M&.% h %\  P%~& h &2 C'e& ."openPC"\PC.reconfigure PCconfig h &3 .t\CC.reconfigure CCconfig ."openPC"\PC.home.seek :open P%$x2 ^22b .t\CC.home.seek :open P%v( C'$x2 ^22b '( +223 h ( +3 ( +160 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 06:03:36 began in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:49:00CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #15) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'u& h & .tHand.open M&1 +150 h 1 M&0& h &_ M&L% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &2(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &['η +91 h 'η .tHand.close M&3/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &G' +91 h ' .t\CC.seek :closed C'$(W +416 h (W C'\ & h &W .tHand.open M&s1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'S  +151 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'E'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '?% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ';'[ +463 h '[ .t\CSV.dial :waste C'k +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '6'4 +2010 h '4 #t\Purging external Intake C'j& h &W C'R% h %4 .t\CSV.dial :flush C'x  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'I  h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'q  +75 h #t\Flushing upper manifold C'(& h &W C'% h %4 .t\CSV.dial :flush C'`  +76 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'w +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'T'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C' % h %0 .t\CS.seek 5ml C'd'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C':'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'& +75 h .t\CS.seek 0ml C'{'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&h& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&C' +91 h ' .tHand.close M&c/ +150 h / .t\CC.seek :open C'P( +416 h ( M&r& h &ӷ M&}% h % .t\Forearm.seek :retract M&!'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +90 h 'η .tHand.open M&y1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:49:04CEST30-Aug-18\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:49:00CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'1& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&m% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(H% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (5(~ +7 F*#% h %&K ."readyMFB"\ATV.dial :waste2 F*9 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *l' +194 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b ()& h &~ M&"& h &- M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Y( +166 h ( M&& h & A(|& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &M'н +89 h 'н A(-& h &~ .t\SE.seek 0.71 Pucks below Top Plate A(Q'K +253 h 'K A( & h &~ M&F& h & .t\Elbow.seek 3 M&( +7 h ( M&Z& h & A(b& h &~ .t\Forearm.seek 3 + 300 counts M&O'- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +150 h / ."downElevator"\SE.seek :down A(%'X M&8& h & M&% h %- .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.seek CC M&7(ӷ +143 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' .t\CC.seek :closed C'~(W +416 h (W .tHand.open M&e1 +150 h 1 M&z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +90 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)i+ +7 h + S)V% h % C'u& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'9 h 9Td +100 C'9 h 9kd +100 C'`9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'D9 h 9d +100 C'9 h 9d +100 C'+9 h 9d +100 C' 9 h 9 d +100 C'69 h 9 d +100 C'(9 h 96d +100 C'<9 h 9Ld +100 C'9 h 9bd +100 C'9 h 9xd +100 C't9 h 9d +100 C'9 h 9d +100 C'N9 h 9d +100 C' 9 h 9d +100 C' 9 h 9d +100 C'T9 h 9d +100 C':9 h 9d +100 C'&9 h 9(d +100 C'h9 h 9>d +100 C'd9 h 9Td +100 C'9 h 9id +100 C'w9 h 9d +100 C'K9 h 9d +100 C'a9 h 9d +100 C'c9 h 9d +100 C'9 h 9d +100 C'$9 h 9d +100 C'}9 h 9  d +100 C'C9 h 9 d +100 C'9 h 9 *d +100 C'f9 h 9 ?d +100 C'A9 h 9 Td +100 C'y9 h 9 j d +100 C'r9 h 9 $d +100 C'*9 h 9 (d +100 C'!9 h 9 ,d +100 C'9 h 9 0d +100 C'm9 h 9 4d +100 C'H9 h 9 8d +100 C'9 h 9 '- +22 h '- .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down M& & A(j'X h & M&S% h %- .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'{(W +416 h (W .tHand.open M&D1 +150 h 1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'6  +151 h  .t\CSV.dial CTV C'~  +19 h  .t\CS.seek 5ml C'N'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C' ' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%T  +38 h  .t\PS.seek 2.5ml P%h' +2609 h ' .t\PSV.dial :mixloop P%d +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%( +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%K  +151 h  .t\PS.seek :empty P%a' +2610 h ' .t\CBV.dial :waste1 C'c @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'$  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'} @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C': ` +19 h ` .t\CSV.dial :air C'& @ +38 h @ .t\CS.seek 10ml C'A' +2647 h ' .t\CSV.dial CTV C'y  +38 h  .t\CS.seek 0ml C'*'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'm  +150 h C'H& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&% h %\  A(#% h %X A(9. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &ӷ M&f% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &C( +143 h ( M&"& h & A(U& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &G' +89 h ' A(\ & h &  .t\SE.seek 19.71 Pucks below Top Plate A(w' +66 h ' M&1& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 2 M&$ ,(#b &#Q@2^d^2b &|' +26 h ' .t\Elbow.hold M&r, +6 h , .t\Elbow.reconfigure TightElbow A(B- M&$&.,(\ #b h -+ .t\SE.seek 20.04 Pucks below Top Plate A(' +27 h ' M& % h % A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&-'; +20 h '; M&& h & A(_& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &^' +22 h ' .t\SE.seek 21.04 Pucks below Top Plate A()' +37 h ' M&Z& h & M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +89 h '\  .t\Elbow.seek CC M&M(ӷ +143 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'O( +416 h ( A(& h &  A(%& h &  M&o& h &ӷ M&e% h % .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &V( +143 h ( M&P& h & A(z& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &u' +92 h ' .t\Elbow.hold M&L, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &'1 +150 h 1 M&5% h % A(g& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &W'; +21 h '; M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +22 h ' A(b% h % ."downElevator"\SE.seek :down A('X +54 h 'X ."downElevator"\SE.coast A(5 +46 (=% h %X M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +90 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%2  +113 h  P%.% h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%0 +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%s  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%/  +19 h  P%p% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %?' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %]' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C';& h & .tHand.open M&R1 +2 h 1 M&E& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&3(- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&+& h &- M& % h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +264 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'I(W +416 h (W C'>& h &W .tHand.open M&1 +150 h 1 M&x% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  C'4& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%D @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%q  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%6  +75 h  .tdelay 2 seconds +200 P%~% h % .t\PS.seek 1.499ml P%`' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%n' +1094 h ' .t\PSV.dial :air P%!  +38 h  .t\PS.seek 0ml P%N' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%T  +38 h  .tdelay 2 seconds +200 P%i% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %d'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%(% h %r .t\PS.seek 0.475ml P%K' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%h  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%c'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%$  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%}  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%: +75 h .t\PTV.dial :PRV,avoiding: :puck P%& @ +76 h @ .t\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial :air P%y  +76 h  .t\PS.seek 2.5ml P%A' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%m' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%# +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%9  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'f & +50 h & .t\AWV.select :waste C' +7 P%"% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %U'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'\ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %1' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%B +38 h .t\PS.seek 0ml P% ' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery F*_ @@T h &W F*Z  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%8 +21 h  ."MFBPCR"delay 20 seconds +92 h P%t% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%G' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*C I +129 h I F*w% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *|'6 +66 .t\PSV.dial :waste1 P%Y  +38 h  .t\PS.seek 0ml P%@' +1597 h ' .t\PSV.dial PSR P%- +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%r' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*Q  +22 h ' .t\PSV.dial PSR P%F +21 h  F*{% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%o' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*e % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *P'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%z  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*M  +22 h  F*'% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *5' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%g +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F* I +42 h I F*b% h % ."MFBPCR"\AS.seek 0.469ml F*J' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%V +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%=' +2610 h ' .t\PSV.dial PSR P%l +113 h P%u% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%W' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%)  +38 h  .t\PS.seek 0ml P%2' +739 h ' ."MFBPCR"delay 5 seconds +500 F*.9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%a +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%0' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +2610 h ' .t\PSV.dial PSR P% +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%/' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%p  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%?' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%R +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %E'k +2679 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %,' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *L'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%+' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %\\' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %7' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*<  +43 h  F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *S'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*O I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%x +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %4' +619 h ' ."MFBPCR"\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.seek :empty F*'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*q  +86 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *~' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*]  +43 h F*`% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *!'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'n  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'T'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'i' +1626 h ' ."connect"\CSV.dial CTV C'N  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'( @ +38 h @ .t\CS.seek 0ml C'd'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*h  +22 h ."MFBPCR"delay 3 seconds +300 F*K% h %_ ."MFBPCR"\AS.seek 0.434ml F*$'ݏ +110 h '4 .t\CSV.dial CSR C'}  +38 h  C'c% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C':'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*&  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*y'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*A'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'H' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'm  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'#'4 +2647 h '4 C'9& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'U( +416 h ( M&& h &ӷ M&1% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Z'η +91 h 'η .tHand.open M&81 +150 h 1 M&t% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'C& h & M&w% h %\  A(Y% h %X A([. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (G(,% +357 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (-& h &,% M&@& h &- M&^% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +166 h (k M&Q& h &k A({& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &F'Ю +89 h 'Ю A(%& h &,% ."WCR"\SE.seek 20.71 Pucks below Top Plate A(|' +56 h ' A(r& h &,% M&e& h &k ."WCR"\Elbow.seek 4 M&(k +7 h (k M&z& h &k A(M& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&'' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &o/ +42 h '&0 ."MFBPCR"delay 3 seconds +108 h / ."downElevator"\SE.seek :down A(P'X M&g& h &k M&% h % ."WCR"\Forearm.seek :retract M&b'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."WCR"\Elbow.seek CC M&(ӷ +101 ."MFBPCR"\ATV.dial :waste2 F*5 % +42 h (ӷ M&V& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' + h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +90 h ' ."WCR"\CC.seek :closed C'=(W +416 h (W ."WCR"Hand.open M&)1 +53 h 'R ."MFBPCR"\ATV.dial :air2 F*W  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *.' +75 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)J+ +7 h + S)a% h % C'2& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +65 h  F*x% h % ."MFBPCR"\AS.seek 0.52ml F*S' +678 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'7% h %4 S)D% h % S)% h % ."WCR"Intake.select :close S)0 +6 )q% h % ."WCR"\SSV.dial :exhaust S)6  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X  +64 h  ."MFBPCR"\ARV.dial :carrier F*x  +11 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +21 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*n' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*T  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F**' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +107 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*c'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +22 h n ."MFBPCR"delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.6ml F*!'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +43 h  ."MFBPCR"\ASV.dial :waste1 F*y  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*m% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254678 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'f  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'9 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\ ' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'k @ +75 h @ +50 S)"% h % ."WCR"\CBV.dial between CSV and waste2b C'I  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'j ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '1  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'8  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'B  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C't  +38 h  C'w% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'[' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'C @ +75 h @ +50 S)Y% h % ."WCR"\CBV.dial between CSV and waste2b C'@  +94 h  C'^% h % ."WCR"\CSV.dial between air and CTV C'- ` +57 h ` ."WCR"\CS.seek 7.288ml C'{' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'%  ."WCR"\CSV.dial CTV C'Q  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'r'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'e  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'M  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'z% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'o' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)F% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'5 ` +56 h ` ."WCR"\CS.seek 7.288ml C'V' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C''  'P  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'|  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'A' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'U  +151 h C'b& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&)% h %\  A(% h %X A(W. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (2(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &~ M&a& ."raiseStack"\SE.seek :upperLimit h &ӷ A(l'Z ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&J/ +150 h / ."WCR"\CC.seek :open C'p( +23 h 'Z +392 ( A(s& h &~ A(/& h &~ M&& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&R'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A(.& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &;' +92 h ' ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &,1 +150 h 1 M&% h % A(E& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M& '- +20 h '- M&<& h & A(\\& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &L'н +22 h 'н A(3% h %Z ."downElevator"\SE.seek :down A('X +100 (>% h % M&x% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&71 +2 A(6 h 1 M&& h & M&D% h %\  .t\Elbow.seek FlushPuck::Garage M&q(- +166 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.seek :garage M&`'η +91 h 'η .tHand.close M&6/ +150 h / M&~& h &- M& % h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +264 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &(' +91 h ' .t\CC.seek :closed C'i(W +416 h (W C'*& h &W .tHand.open M&K1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'h& h &W C'd% h % .t\CSV.dial CSR C'c  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'&'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '!% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '$'[ +462 h '[ .t\CSV.dial :waste C'y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'm& h &W C' % h %4 .t\CSV.dial :flush C':  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'9% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  'k  +38 h  +113  .t\CBV.dial :waste1 C'I @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'j& h &W C'\ % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'1'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'B  +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'[% h %4 .t\CSV.dial :flush C'C  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'^% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z 'Q  +38 h +75 .t\CTV.dial :air C'%  +151 h .t\CS.seek 0ml C'{'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'e'0 +506 h '0 .t\CSV.dial :waste C'r +38 h .t\CS.seek 0ml C'M'4 +506 h '4 C' & h &W C'z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'o'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'F% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +506 h '0 .t\CSV.dial :waste C'g +75 h .t\CS.seek 0ml C'''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'P& h &W C'V% h %4 .t\CSV.dial CSR C'|  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'b' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C') +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'W' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'u  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  '=  +38 h  +113  .t\CBV.dial :waste1 C's @ +37 h @ .t\CS.seek 0ml C'/'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'G  +75 h  .t\CS.seek 0ml C','4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'E% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +710 h ' ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV C'R  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'>'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +506 h '0 .t\CSV.dial :waste C'; +38 h .t\CS.seek 0ml C'+'4 +507 h '4 #t\Flushing upper manifold C'7& h &W C'% h %4 .t\CSV.dial :flush C'D  +37 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C'L% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'2'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'6'4 +507 h '4 C'~& h &W C' % h %4 .t\CSV.dial :flush C'?  +37 h  .t\CS.seek 3ml C'`'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C'n% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'S'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +507 h '0 .t\CSV.dial :waste C'* +75 h .t\CS.seek 0ml C'K'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'}& h &W C'h% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'(% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CTV C'!  .t\CTV.dial CSV C'&  +38 h  +112  .t\CBV.dial :waste1 C'y @ +38 h @ .t\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'm& h &W C' % h %4 .t\CSV.dial :flush C':  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'9% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'k  +75 h  .t\CS.seek 0ml C'I'4 +766 h '4 #tDisinfecting external Intake with kill C'#& h &W C'j% h %4 .t\CSV.dial CSR C'\  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 '1  +38 h +75 .t\CTV.dial :intake C'B  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C't' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 .t\CSV.dial CSR C'[  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'w'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +1372 h ' .t\CSV.dial CBV C'^ +113 h .t\CS.seek 0ml C'Y'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&Q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +7 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C'e( +416 h ( M& & h &ӷ M&z% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &o'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&P1 +2 h 1 M&V& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &|(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.seek :garage M&U'η +91 h 'η .tHand.close M&)/ +150 h / M&& h &- M&b% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &a' +91 h ' .t\CC.seek :closed C'J(W +416 h (W C'X& h &W .tHand.open M&=1 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'/  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'G% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '.'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'E'4 +2009 h '4 #t\Purging external Intake C',& h &W C'<% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'\\  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'>'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C';  +75 h #t\Flushing upper manifold C'x& h &W C'7% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'D'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +37 h @ C'l% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C']'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'2& h &W C'~% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'?'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'N% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'`'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'S +75 h .t\CS.seek 0ml C'*'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&p& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&K' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'(( +416 h ( M&c& h &ӷ M&!% h % .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +264 h (- M&A& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&:1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Completed [mbaPhase] begun 06:03:36 #\Preparing to sleep until Sat Oct 13 06:03:36 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:03CEST13-Oct-18 #\Continuing at 06:03CEST13-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:50:29CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:03CEST13-Oct-18" +235818 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1109h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*"  +22 h ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +21 h F*\ % h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +65 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *C'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*F'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*|'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b */'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*G  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*.  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*<'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*R  +108 h  ."MFBPCR"delay 7 seconds +700 F*E% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +22 h n ."MFBPCR"delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*L % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1111h25ml.pcr F*]% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*O  +21 h ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*N'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*`'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*q% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*:% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*C  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*r  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.53ml F*Z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*5% h %< ."MFBPCR"\AS.seek 0.594ml F*P'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*V n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1113h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*u  +22 h ."MFBPCR"\ATV.dial :air3 F*X % +86 h % F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*=% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*G'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Y% h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*R% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *E'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*L'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*2% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*}  +22 h ."MFBPCR"delay 2 seconds +200 F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *A'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*& n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*$' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*9 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*_' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*k % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*"'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*# n +21 h n ."MFBPCR"delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.65ml F*B' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1116h25ml.pcr F*[% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F*^ % +86 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *r'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*z% h %n ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*F  +64 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*)  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*e  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct1118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ARV.dial :carrier F*.  +11 h  F*E% h %V ."MFBPCR"\AS.seek 0.5ml F*<'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*;  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +21 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *7'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*0  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*L' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*O  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*?'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*n  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*h% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct1118h25ml.pcr F*d% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*c  +21 h ."MFBPCR"\ATV.dial :air3 F*J % +87 h % F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*:% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*$'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*8  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +21 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *T'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*5'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*)  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +22 h ."MFBPCR"delay 2 seconds +200 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*U I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*M' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*g % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*u  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*e  +21 h  ."MFBPCR"delay 7 seconds +700 F*s% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*= n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*.  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*E % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*<  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*\\  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *;' +424 h ' ."MFBPCR"\ATV.dial :ASV F*> n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*+  +43 h  F*x% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *7'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *0'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*]'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*l % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *2' +424 h ' ."MFBPCR"\ATV.dial :air3 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*D% h % ."MFBPCR"\AS.seek 0.71ml F*6' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*n' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*?% h % Ű ."MFBPCR"\AS.seek 0.72ml F*q' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*` n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*h% h % zG ."MFBPCR"\AS.seek 0.365ml F*S' +7162 h ' ."MFBPCR"\ATV.dial :waste F*p n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*d % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *c' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +21 h F*!% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %  ."MFBPCR"\AS.seek 0.604ml F*m'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*K' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*9 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*H  +21 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*_ % +107 h % F*$% h %V ."MFBPCR"\AS.seek 0.01ml F*j' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*"% h % ."MFBPCR"\AS.seek 0.58ml F*#' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h % q ."MFBPCR"\AS.seek 0.605ml F*8'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*1% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*B  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *['V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*C  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *@' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*w n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +201 h 'R ."MFBPCR"\SPE.coast F*T  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*%'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*r n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *-' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *{': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*F% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *5'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*V n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*) I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*P  +43 h  F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*o  +65 h  F*g% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *|'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*M n +64 h n F*u% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *X' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*s  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*= n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*/'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*a % +43 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F* % h % ."MFBPCR"\AS.seek 0.96ml F*.'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*<% h %0 ."MFBPCR"\AS.seek 0.985ml F*R' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +21 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*3'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*> I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *x'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *7'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*0  +43 h  F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *]' +9988 h ' ."MFBPCR"\ATV.dial AIV F*L I +43 h I ."MFBPCR"\AS.seek 0.02ml F*2' +9880 h ' ."MFBPCR"\ATV.dial :waste F*~ n +128 h n ."MFBPCR"\AS.seek 0ml F*D'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*J  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +65 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*j'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*\ % h %m ."MFBPCR"\AS.seek 0.82ml F*#'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' @CEST1539295219.54 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *@'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*Q % +107 h % ."MFBPCR"\AS.seek 0ml F*r'V +2548 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539403416.22 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )  h  C' ' h  P% %% h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *-  h  .t\SPE.configure SPEconfig F*\ 2 *z% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'Z& S)#.0X 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:51:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #16) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&R1 +150 h 1 M&>& h &_ M&3% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &+(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&/ +150 h / M&0& h &- M&l% h %η .t\Forearm.seek :retract M&7'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +265 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'2& h &W .tHand.open M&D1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'N& h &W C' % h %4 .t\CSV.dial CSR C'n  +150 h  C'`% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '?'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'p'[ +463 h '[ .t\CSV.dial :waste C'h +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'd'4 +2009 h '4 #t\Purging external Intake C'& h &W C'S% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c 'W  h +75 .t\CTV.dial :intake C'}  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C':'4 +910 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'&& h &W C' % h %4 .t\CSV.dial :flush C'm  +75 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'A% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'_ +113 h .t\CTV.dial :air .\CS.seek 0ml C'$'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'k'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'"'4 +507 h '4 C'& h &W C'I% h %4 .t\CSV.dial :flush C'\  +37 h  .t\CS.seek 3ml C'j'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'#% h %0 .t\CS.seek 5ml C'1'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'8'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'B +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'T( +416 h ( M&w& h &ӷ M&Q% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +264 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &('η +91 h 'η .tHand.open M&1 +150 h 1 M&v% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:51:31CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:51:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F**% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M& % ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(%. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +6 F*-% h %&K ."readyMFB"\ATV.dial :waste2 F*z % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +350 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (Z& h &,% M&i& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &F(k +166 h (k M&{& h &k A(V& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +90 h 'Ю A(5& h &,% .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(& h &,% M&'& h &k .t\Elbow.seek 4 M&P(k +6 h (k M&U& h &k A(g& h &,% .t\Forearm.seek 4 + 300 counts M&b' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &M/ +150 h / ."downElevator"\SE.seek :down M&u& A(o'X h &k M&|% h % .t\Forearm.seek :retract M&X'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &G' +91 h ' .t\CC.seek :closed C'E(W +416 h (W .tHand.open M&<1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)a+ +6 h + S)/% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  C'3 @ +113 h @ +38  .tdelay 3.8 seconds +205 h ' ."readyMFB"\AFV.select :carrierSolution F* +6 ."readyMFB"delay 2 seconds +169 C'% h %4 S)x% h %5J .t\SSV.dial :spit S); +31 ."readyMFB"\AS.seek 1ml F*Y' +119 h .t\SS.seek 0ml S)' +216 h ' .t\SSV.dial CBV S)0  +151 h  .t\CTV.dial :intake C'l  +75 h  #tWaiting for external sampler to finish priming... .delayUntil "06:12:15.14" +4724 h ' ."readyMFB"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."readyMFB"delay 1 second +100 .\ATV.dial :waste F* n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*L  h  ."readyMFB"\ASV.dial :waste1 F*]  h +18553 ."connect"\SSV.dial CBV .t\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'+% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X   '9 h 9d +100 C'x9 h 9d +100 C';9 h 91d +100 C'9 h 9J d +100 C'R9 h 9bd +100 C'09 h 9zd +100 C'l9 h 9d +100 C' 9 h 9d +100 C'Y9 h 9 d +100 C'L9 h 9$d +100 C']9 h 9(d +100 C'+9 h 9 ,d +100 C'9 h 9$0d +100 C'\\9 h 9<4d +100 C'29 h 9S8d +100 C'D9 h 9kd +100 C' 9 h 9Td +100 C'}9 h 9kd +100 C'm9 h 9d +100 C':9 h 9d +100 C'A9 h 9d +100 C'H9 h 9d +100 C'_9 h 9d +100 C'99 h 9d +100 C'$9 h 9 d +100 C'd9 h 9 d +100 C'&9 h 96d +100 C'k9 h 9Ld +100 C'9 h 9bd +100 C'I9 h 9xd +100 C'K9 h 9d +100 C'"9 h 9d +100 C'9 h 9d +100 C'#9 h 9d +100 C'\ 9 h 9d +100 C'j9 h 9d +100 C'89 h 9d +100 C'B9 h 9(d +100 C'9 h 9>d +100 C'19 h 9Td +100 C'[9 h 9id +100 C'9 h 9d +100 C'C9 h 9d +100 C't9 h 9d +100 C'@9 h 9d +100 C'69 h 9d +100 C'9 h 9d +100 C'Q9 h 9  d +100 C'49 h 9 d +100 C'O9 h 9 *d +100 C'^9 h 9 ?d +100 C'f9 h 9 Td +100 C'9 h 9 j d +100 C'w9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'*9 h 9 0d +100 C' 9 h 9 4d +100 C'9 h 9 8d +100 C'%9 h 9 % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'D& h & M&% h %\  A(N% h %X A(7. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (`(,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (e& h &,% M&q& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &h(k +166 h (k M&p& h &k A(S& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +89 h 'Ю A(& h &,% .t\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(W& h &,% M&!& h &k .t\Elbow.seek 4 M& (k +7 h (k M&}& h &k A(:& h &,% .t\Forearm.seek 4 + 300 counts M&A' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &9/ +150 h / ."downElevator"\SE.seek :down M&$& A('X h &k M&n% h % .t\Forearm.seek :retract M&J'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&c(ӷ +142 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&H1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'd  +151 h  .t\CSV.dial CTV C'E  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'#' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'\ '4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%j  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%1 +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%I  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%& +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%C  +150 h  .t\PS.seek :empty P%t' +2610 h ' .t\CBV.dial :waste1 C'@ @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'Q @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'O  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'8 ` +19 h ` .t\CSV.dial :air C'B @ +38 h @ .t\CS.seek 10ml C'f' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'['4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'6  +150 h C'*& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M& % h %\  A(% h %X A(%. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (-(~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (z& h &~ M&_& h &ӷ M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&Z& h & A(^& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~'н +89 h 'н A(2& h &~ .t\SE.seek 0.71 Pucks below Top Plate A('K +254 h 'K M&& h & A({& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &V' +25 h ' .t\Elbow.hold M&w, +7 h , .t\Elbow.reconfigure TightElbow A(i- M&$&.,(\ #b h -+K .t\SE.seek 1.04 Pucks below Top Plate A(y' +27 h ' M&'% h % A(v& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &5'- +21 h '- M&"& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &u'н +22 h 'н .t\SE.seek 2.04 Pucks below Top Plate A((' +36 h ' M&g& h & M&|% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&)/ +150 h / .t\CC.seek :open C'b( +416 h ( A(o& h &~ A(X& h &~ M&s& h &ӷ M&<% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &/( +142 h ( M&T& h & A(& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&3, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &=1 +150 h 1 M&% h % A(x& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&;'- +21 h '- M&R& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &M'н +22 h 'н A(r% h % ."downElevator"\SE.seek :down A(l'X +100 ( % h %B M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%+  + A(6 +112 h  P%G% h % .t\PS.seek 0.499ml P%\\' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%> +38 h .t\PRV.dial :air P%Y  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%D  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%N  +19 h  .t\PRV.dial :air P%7  +19 h  P%e% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %q' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %L' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'p& h & .tHand.open M&S1 +2 h 1 M&& h & M&0% h %\  .t\Elbow.seek FlushPuck::Garage M&W(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&/ +150 h / M&9& h &- M&$% h %η .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'A(W +416 h (W C'?& h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%H @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%d  +75 h  .tdelay 2 seconds +200 P%E% h % .t\PS.seek 1.499ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%`' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%# +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%j  +38 h  .tdelay 2 seconds +200 P%a% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %1'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%I  +75 h .tdelay 2 seconds +200 P%&% h %r .t\PS.seek 0.475ml P%C' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%@'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%Q  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %O' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%8 +75 h .t\PTV.dial :PRV,avoiding: :puck P%B @ +76 h @ .t\PS.seek 0ml P%\ ' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%f' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%6' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%*  +76 h  .t\PS.seek 2.5ml P% ' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%%  +75 h P%z% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %_' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'4 & +50 h & .t\AWV.select :waste C' +7 P%Z% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %^'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'2 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %c' +1313 h ' .t\PSV.dial :air P%K  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%{ +38 h .t\PS.seek 0ml P%i' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 ''& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'v& F*" @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%g +22 h  ."MFBPCR"delay 20 seconds +91 h P%|% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%~' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +128 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *V'6 +66 .t\PSV.dial :waste1 P%F  +38 h  .t\PS.seek 0ml P%,' +1597 h ' .t\PSV.dial PSR P%5 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%u +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%w' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*)  +22 h ' .t\PSV.dial PSR P%U +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*o'ͯ +92 h P%X% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%s' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*< % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%x +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%R' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*r% h % ."MFBPCR"\AS.seek 0.469ml F*P' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%;' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  .t\PS.seek 0ml P%+' +740 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*M n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*l% h % ."MFBPCR"\AS.seek 0.041ml F*-'% +336 h ' .t\PSV.dial PSR P%t +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%Y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial PSR P% +113 h P%N% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%7' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%\\' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%q' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%S +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%0  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %!' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*} I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *3'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%9' +2609 h ' .t\PSV.dial :mixloop P%$ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %]' +5107 h ' .t\PSV.dial :air P%:  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*k  +43 h  F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *n'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*b I +43 h I F*?% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*H n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +85 h  F*d% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *E' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*L  +43 h F*m% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'`  +38 h  C'#% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'j'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'a' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'I  +37 h  +113  .t\CBV.dial :waste1 C'& @ +38 h @ .t\CS.seek 0ml C'1'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*C% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'Q  +38 h  C'@% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'8'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*B  +21 h F*O% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*\ n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*f'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'*' +1626 h ' ."connect"\CSV.dial CTV C'6  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2648 h '4 C'%& h &W #tGaraging FlushPuck in Collection Clamp M&z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C'^( +416 h ( M&K& h &ӷ M&c% h % .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&g1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'& h & M&% h %\  A(F% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (~(,% +201 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (5& h &,% M&,& h &- M&u% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &y(k +165 h (k M&)& h &k A(& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &U'Ю +90 h 'Ю A(X& h &,% ."WCR"\SE.seek 17.71 Pucks below Top Plate A(V'* +86 h '* A(w& h &,% M&<& h &k ."WCR"\Elbow.seek 4 M&o(k +7 h (k M&& h &k A(& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&=' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &s/ +150 h / ."downElevator"\SE.seek :down A(T'X M&x& h &k M&% h % ."WCR"\Forearm.seek :retract M&r'\ +18 h '&0 ."MFBPCR"delay 3 seconds +68 h 'X ."downElevator"\SE.coast A(5 +5 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &R' +85 ."MFBPCR"\ATV.dial :waste2 F* % +6 h ' ."WCR"\CC.seek :closed C'(W +37 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +379 h (W ."WCR"Hand.open M&(1 +150 h 1 M&;% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +31 h 'R ."MFBPCR"\ATV.dial :air2 F*M  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *l' +39 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)P+ +7 h + S)t% h % C'+& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'Y  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'5J +216 h '5J ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV C'N @ S)  +151 h  @ ."WCR"delay 3.8 seconds +380 C'D% h %4 S)e% h %5J ."WCR"\SSV.dial :spit S)7 +150 h ."WCR"\SS.seek 0ml S) ' +216 h ' ."WCR"\SSV.dial CBV S)\\  +151 h  ."WCR"\CTV.dial :intake C'-  +75 h  #"WCR"Waiting for external sampler to finish priming... .delayUntil "08:38:37.94" +18613 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*S n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*q' +9327 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'0% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*- I +86 h I ."MFBPCR"\AS.seek 0ml F*S'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*l'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*0  +43 h  #"MFBPCR"\Swish #1 of 2 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *}'\  +1031 h '\  F*p% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*!% h %(A ."MFBPCR"\AS.seek 0.6ml F*$'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*]'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*k% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *W' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*3 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +3472 h 'V ."MFBPCR"\SPE.coast F*:  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*?  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*q% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*b  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *n'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*E  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*`' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*j  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*[' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*& % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*I'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +107 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*@'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*\ ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*6% h % \\ ."MFBPCR"\AS.seek 0.326ml F**'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254552 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'4  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'% @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'h @ +76 h @ +50 S)Z% h % ."WCR"\CBV.dial between CSV and waste2b C'A  +94 h  C'K% h % ."WCR"\CSV.dial between air and CTV C'J ` +18 h ` ."WCR"\CS.seek 7.288ml C'_' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'c  ''  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'g  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'v' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'{  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'|  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C' ' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)F% h % ."WCR"\CBV.dial between CSV and waste2b C'u  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C', ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'X  ')  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'w'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'<  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'i  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C's' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'y @ +75 h @ +50 S)U% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'/% h % ."WCR"\CSV.dial between air and CTV C'V ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'T  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'o'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'=  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '^' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'r& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&;% h %\  A(M% h %X A((. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (+(~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Y& h &~ M&t& h &ӷ M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &~( +142 h ( M&R& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &N'н +90 h 'н A(P& h &~ ."WCR"\SE.seek 1.71 Pucks below Top Plate A(e': +244 h ': M&D& h & A(7& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &-' +25 h ' ."WCR"\Elbow.hold M&\\, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(S- M&$&.,(\ #b h -+: ."WCR"\SE.seek 2.04 Pucks below Top Plate A(0' +27 h ' M& % h % A(l& h &~ ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &p'- +21 h '- M&}& h & A(!& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н ."WCR"\SE.seek 3.04 Pucks below Top Plate A(' +36 h ' M&$& h & M&k% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +89 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +143 h (ӷ M&W& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ ."WCR"\Forearm.seek CC M&?' +91 h ' ."WCR"Hand.close M& / +150 h / ."WCR"\CC.seek :open C'q( +416 h ( A(9& h &~ A(& h &~ M&& h &ӷ M&H% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &b( +142 h ( M&L& h & A(m& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &d' +93 h ' ."WCR"\Elbow.hold M&E, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b  +150 h 1 M&n% h % A(j& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'- +20 h '- M&`& h & A(&& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &a'н +23 h 'н A([% h % ."downElevator"\SE.seek :down A(C'X +100 (Q% h %1 M&I% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&@1 +2 A(6 h 1 M&O& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&6/ +150 h / M&\ & h &- M&8% h %η .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +264 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C'A& h &W .tHand.open M&K1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'c& h &W C''% h % .t\CSV.dial CSR C'_  +38 h  C'g% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '{% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '|'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'v'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'% h %4 .t\CSV.dial :flush C'F  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C',% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  ')  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'<'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'w& h &W C'% h %4 .t\CSV.dial :flush C'i  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C's% h %p .t\CS.seek 7ml C'y'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'/'4 +766 h '4 #t\Flushing upper manifold C'V& h &W C'x% h %4 .t\CSV.dial :flush C'T  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'=% h %p .t\CS.seek 7ml C'o'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f 'r  +38 h +75 .t\CTV.dial :air C';  +151 h .t\CS.seek 0ml C'M'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +506 h '0 .t\CSV.dial :waste C'Y +38 h .t\CS.seek 0ml C't'4 +507 h '4 C' & h &W C'R% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'P'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'G% h %0 .t\CS.seek 5ml C'D'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'7'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'l'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'}& h &W C'!% h %4 .t\CSV.dial CSR C'$  +151 h  C'k% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'N'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'~' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'e +113 h .t\CTV.dial :intake C'-  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'W' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C':  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'0  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  ']  +38 h  +113  .t\CBV.dial :waste1 C'9 @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'H% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'L'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  +38 h  .t\CBV.dial :waste2b C'3  +75 h  .t\CS.seek 0ml C'#'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'n  +37 h  C'j% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '`'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'[ '  +37 h +76 .t\CTV.dial :air C'b  +150 h .t\CS.seek 0ml C'Q'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +507 h '0 .t\CSV.dial :waste C'1 +37 h .t\CS.seek 0ml C'@'4 +507 h '4 #t\Flushing upper manifold C'O& h &W C'B% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'a .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'E +37 h .t\CS.seek 0ml C'6'4 +507 h '4 C'\ & h &W C'8% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'A +75 h .t\CS.seek 0ml C'K'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'J& h &W C'c% h %4 .t\CSV.dial :flush C''  +37 h  .t\CS.seek 5ml C'_'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'g @ +38 h @ C'h% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'|'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'% h %4 .t\CSV.dial :flush C'F  +76 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C',% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV C')  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'X  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'5& h &W C'w% h %4 .t\CSV.dial CSR C'  +38 h  C'i% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'<'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 's  +37 h +75 .t\CTV.dial :intake C'U  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'y  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'V'4 +606 h '4 .t\CSV.dial CSR C'x  +113 h  C'T% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'/'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'= +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&r& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &f(ӷ +7 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&Y/ +150 h / .t\CC.seek :open C'(( +416 h ( M& & h &ӷ M&R% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &P'η +91 h 'η .tHand.open M&D1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&}1 +2 h 1 M&!& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &$(- +7 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&e/ +150 h / M&-& h &- M&~% h %η .t\Forearm.seek :retract M&:'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +265 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'& h &W .tHand.open M&]1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'H% h %4 .t\CSV.dial CSR C'  +113 h  C'L% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'?'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '3% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'm'[ +463 h '[ .t\CSV.dial :waste C'n +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +2010 h '4 #t\Purging external Intake C'#& h &W C'`% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'b .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'&  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'Q'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'1  +75 h #t\Flushing upper manifold C'I& h &W C'O% h %4 .t\CSV.dial :flush C'B  +75 h  .t\CS.seek 5ml C'd'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'a'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'C +37 h .t\CS.seek 0ml C'E'4 +507 h '4 C'+& h &W C'\ % h %4 .t\CSV.dial :flush C'8  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'%% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'z +75 h .t\CS.seek 0ml C'A'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +6 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.seek CC M&K' +91 h ' .tHand.close M&g/ +150 h / .t\CC.seek :open C'h( +416 h ( M&_& h &ӷ M&{% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&F1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 06:03:51 #\Preparing to sleep until Mon Oct 15 06:03:51 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:03CEST15-Oct-18 #\Continuing at 06:03CEST15-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:52:58CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:03CEST15-Oct-18" +235143 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1309h25ml.pcr F*,% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*)  +22 h ."MFBPCR"\ATV.dial :air3 F*X % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *5'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*s'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*x% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *T'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +31 h  ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*R'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*G'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*D% h %m ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*0  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*9  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*L  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*p% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*3  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*m  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.536ml F*`'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*& n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*B' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*d'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1311h25ml.pcr F*a% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*+ % +86 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*%'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +65 h  F*g% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*J % +86 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*u% h %m ."MFBPCR"\AS.seek 0.82ml F*v'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*w  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*T  +10 h  F*V% h %V ."MFBPCR"\AS.seek 0.5ml F*/'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +22 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Z'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*M' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*f' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*D  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*7  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*}'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*P  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.65ml F*-' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*N% h % \\ ."MFBPCR"\AS.seek 0.326ml F*0'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1313h25ml.pcr F*$% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*:  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +21 h F*]% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*Q % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*d'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*_ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*J  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *^'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*, I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.53ml F*"' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*X % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*w  +107 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*<'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*s  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*V % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1316h25ml.pcr F*x% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Y'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*R% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*D  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +65 h  F*f% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *7'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*l % +87 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*-% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*0'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*]' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct1318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*`'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Q  +22 h ."MFBPCR"delay 2 seconds +200 F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*B n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*>  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*d'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +22 h  ."MFBPCR"delay 7 seconds +700 F*\ % h %< ."MFBPCR"\AS.seek 0.594ml F*E'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +21 h n ."MFBPCR"delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*g'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct1318h25ml.pcr F*'% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*_  +21 h ."MFBPCR"\ATV.dial :air3 F*\\ % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*^'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F**% h %n ."MFBPCR"\AS.seek 0.82ml F*|'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*T'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ARV.dial :carrier F*M  +10 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*l  +22 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*N I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*-% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*:  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +21 h  ."MFBPCR"delay 7 seconds +700 F*9% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*] n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*p'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*3  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*#% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*n @@T ."MFBPCR"\ASV.dial :SPEdelivery F*`  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*&  +150 h F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *1' +424 h ' ."MFBPCR"\ATV.dial :ASV F*Q n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*@  +43 h  F*I% h % ."MFBPCR"\AS.seek 0.62ml F*B'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *>'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*a'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +42 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*d% h % ."MFBPCR"\AS.seek 0.71ml F*6' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*c% h % zG ."MFBPCR"\AS.seek 0.365ml F*z' +7161 h ' ."MFBPCR"\ATV.dial :waste F*q n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*' % +43 h % F*g% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *_' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*{% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*K' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*, n +86 h n ."MFBPCR"\ASV.dial :wasteR F**  +21 h  ."MFBPCR"\AS.seek 0ml F*^'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *u'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*" % +108 h % F*|% h %V ."MFBPCR"\AS.seek 0.01ml F*w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*)% h % ."MFBPCR"\AS.seek 0.58ml F*5' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*s% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*i' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AS.seek 0ml F*y'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *x'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*T  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*= n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*/ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *r'R +202 h 'R ."MFBPCR"\SPE.coast F*4  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*Y n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *o' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*D  +43 h  F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*7  +65 h  F*G% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *S'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*! n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *k' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*e I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*}  +43 h  F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *-': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*P  +65 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *$'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*t n +64 h n F*:% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*9  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *0': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*] n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*L  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*p % +42 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +21 h F*?% h % ."MFBPCR"\AS.seek 0.96ml F*m'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +21 h % ."MFBPCR"delay 3 seconds +300 F*`% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*b% h % ."MFBPCR"\AS.seek 0.97ml F*['c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*Q I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *I'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*>  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *a' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*+' +9880 h ' ."MFBPCR"\ATV.dial :waste F*\ n +129 h n ."MFBPCR"\AS.seek 0ml F*d'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*C% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F**  +32 h  ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*^% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*u'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*<  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' @CEST1539468037.67 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*r % +107 h % ."MFBPCR"\AS.seek 0ml F*Y'V +2548 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast S)5 P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539576231.66 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )  h  C' '2 h  P% %; h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *o  h  .t\SPE.configure SPEconfig F*\ 2 *R% h %V .t\AS.configure ASconfig S)D% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'f& S)#.0X 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:53:55CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #17) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'b& h & .tHand.open M&1 +150 h 1 M&Q& h &_ M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &@(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&B/ +150 h / M&>& h &- M&% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\ ' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'+& h &W .tHand.open M&d1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'%& h &W C'8% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'q'[ +462 h '[ .t\CSV.dial :waste C'c +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +2010 h '4 #t\Purging external Intake C'g& h &W C'z% h %4 .t\CSV.dial :flush C'\\  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'_ 'W  h +75 .t\CTV.dial :intake C'J  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'F'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'*'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +37 h @ C'^% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'" +113 h .t\CTV.dial :air .\CS.seek 0ml C'|'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +507 h '0 .t\CSV.dial :waste C'v +38 h .t\CS.seek 0ml C')'4 +506 h '4 C'u& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'w'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'5% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'U +75 h .t\CS.seek 0ml C'i'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +6 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&=' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'4( +416 h ( M&/& h &ӷ M&r% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &.'η +91 h 'η .tHand.open M&h1 +150 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:54:01CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:53:55CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Homing MFB Syringe C'n& ."readyMFB"\AFV.select :holdingCoil h & F* M& % h %\  A(2% h %X A(;. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +7 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F*R % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *D' +350 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (f& h &,% M&Z& h &- M&7% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(k +166 h (k M&M& h &k A(!& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +89 h 'Ю A(S& h &,% .t\SE.seek 16.71 Pucks below Top Plate A(e'; +96 h '; A(k& h &,% M&l& h &k .t\Elbow.seek 4 M&}(k +6 h (k M&N& h &k A( & h &,% .t\Forearm.seek 4 + 300 counts M&P' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down M&~& A(:'X h &k M&$% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&H(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' +91 h ' .t\CC.seek :closed C'j(W +416 h (W .tHand.open M&`1 +150 h 1 M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)p+ +6 h + S)% h % C'b& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +151 h  .t\CTV.dial :intake C'  +75 h  #tWaiting for external sampler to finish priming... .delayUntil "06:12:30.88" +4694 h ' ."readyMFB"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."readyMFB"delay 1 second +100 .\ATV.dial :waste F*? n +107 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5065 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*  h  ."readyMFB"\ASV.dial :waste1 F*a  h +18582 ."connect"\SSV.dial CBV .t\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'@% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X9 h 9zd +100 C'9 h 9d +100 C'?9 h 9d +100 C'9 h 9 d +100 C'9 h 9$d +100 C'a9 h 9(d +100 C'@9 h 9 ,d +100 C'B9 h 9$0d +100 C'&9 h 9<4d +100 C'+9 h 9S8d +100 C'd9 h 9kd +100 C'9 h 9Td +100 C'_9 h 9kd +100 C' 9 h 9d +100 C'F9 h 9d +100 C'^9 h 9d +100 C'*9 h 9d +100 C'"9 h 9d +100 C',9 h 9d +100 C'|9 h 9 d +100 C''9 h 9 d +100 C' 9 h 96d +100 C'X9 h 9Ld +100 C'u9 h 9bd +100 C'9 h 9xd +100 C'K9 h 9d +100 C')9 h 9d +100 C'<9 h 9d +100 C'59 h 9d +100 C'9 h 9d +100 C'w9 h 9d +100 C'9 h 9d +100 C'U9 h 9(d +100 C'y9 h 9>d +100 C's9 h 9Td +100 C'x9 h 9id +100 C'v9 h 9d +100 C'T9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'69 h 9d +100 C'C9 h 9d +100 C'r9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'=9 h 9 ?d +100 C'Y9 h 9 Td +100 C'h9 h 9 j d +100 C'/9 h 9 $d +100 C'V9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C' 9 h 9 4d +100 C'29 h 9 8d +100 C';9 h 9 '4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'?  +19 h C'% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'a  +38 h C'@% h %p .t\CS.seek 7ml C'B'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'& @ +38 h @ .t\CS.seek 10ml C'+' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'd ` +132 h ` #tLysing Complete C'E& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&%% h %\  A(O% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (\ (~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b ((& h &~ M&& h &ӷ M&8% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&c& h & A(q& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &g'н +89 h 'н A(z& h &~ .t\SE.seek 2.71 Pucks below Top Plate A(\\') +235 h ') M&& h & A(W& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &J' +25 h ' .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow A({- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(i' +27 h ' M&% h % A(_& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M& '- +21 h '- M&F& h & A(^& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &*'н +22 h 'н .t\SE.seek 4.04 Pucks below Top Plate A("' +36 h ' M&,& h & M&|% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +90 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C')( +416 h ( A(<& h &~ A(5& h &~ M&& h &ӷ M&w% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &U( +143 h ( M&y& h & A(s& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &x' +92 h ' .t\Elbow.hold M&v, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &T1 +150 h 1 M&% h % A(& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &6'- +21 h '- M&C& h & A(r& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н A(% h % ."downElevator"\SE.seek :down A(='X +100 (Y% h %  M&h% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +89 h '\  +32 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'V @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'2& h & .tHand.open M&;1 +2 h 1 M&o& h & M&R% h %\  .t\Elbow.seek FlushPuck::Garage M&A(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.seek :garage M&Z'η +91 h 'η .tHand.close M&n/ +150 h / M&M& h &- M&!% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &N' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'k& h &W .tHand.open M&~1 +150 h 1 M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%G' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%# +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%9' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%P  C'e  h +75 .t\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%:' +2610 h ' .t\PSV.dial :air P%`  +37 h  .t\PS.seek 2.5ml P%4' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%b' +2610 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :mixloop P%[ +37 h .t\PS.seek 0ml P%p' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +6 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&D' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&?% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +265 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&&1 +150 h 1 M&Q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +90 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'd& h & M&E% h %\  A(%% h %X A(O. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +201 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b ((& h &,% M&& h &- M&8% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &c(k +166 h (k M&q& h &k A(z& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &H'Ю +89 h 'Ю A(0& h &,% .t\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A(W& h &,% M&{& h &k .t\Elbow.seek 4 M&(k +6 h (k M&_& h &k A(F& h &,% .t\Forearm.seek 4 + 300 counts M&^' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &,/ +150 h / ."downElevator"\SE.seek :down A(|'X M&g& h &k M&% h % .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.seek CC M&J(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'i(W +416 h (W .tHand.open M&*1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C''  +150 h  .t\CSV.dial CTV C'j  +19 h  .t\CS.seek 5ml C'<'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'5' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%w  +37 h  .t\PS.seek 2.5ml P%y' +2610 h ' .t\PSV.dial :mixloop P%s +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%T  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'C  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'r @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'U @ +37 h @ .t\CS.seek 10ml C'Y' +2647 h ' .t\CSV.dial CTV C'h  +38 h  .t\CS.seek 0ml C'V'4 +2647 h '4 .t\CBV.dial :waste1 C'x @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&2% h %\  A(;% h %X A(o. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (R(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (6& h &~ M&"& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &L( +142 h ( M&f& h & A(\ & h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &='н +90 h 'н A(+& h &~ .t\SE.seek 3.71 Pucks below Top Plate A(v' +224 h ' M&n& h & A(M& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &!' +25 h ' .t\Elbow.hold M&/, +6 h , .t\Elbow.reconfigure TightElbow A(Z- M&$&.,(\ #b h -+ .t\SE.seek 4.04 Pucks below Top Plate A(' +27 h ' M&l% h % A(A& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&S'- +21 h '- M&)& h & A(k& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~'н +22 h 'н .t\SE.seek 5.04 Pucks below Top Plate A(.' +36 h ' M& & h & M&$% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&P/ +150 h / .t\CC.seek :open C'e( +416 h ( A(}& h &~ A(& h &~ M&9& h &ӷ M&`% h % .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&4& h & A(-& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &b' +93 h ' .t\Elbow.hold M&[, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &]1 +150 h 1 M&% h % A(I& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &1'- +20 h '- M&& h & A(7& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &t'н +23 h 'н A(% h % ."downElevator"\SE.seek :down A('X +100 (?% h % M&D% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%@  + A(6 +112 h  P%3% h % .t\PS.seek 0.499ml P%&' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%Q +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%d  +37 h .t\CBV.dial AIV C'E  +38 h .t\PRV.dial between CBV and air P%%  +19 h  .t\PRV.dial :air P%O  +19 h  P%(% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%8 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&z1 +2 h 1 M&0& h & M&>% h %\  .t\Elbow.seek FlushPuck::Garage M&W(- +166 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&F'η +91 h 'η .tHand.close M&B/ +150 h / M&,& h &- M&g% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +264 h (ӷ M&H& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'^& h &W .tHand.open M&1 +150 h 1 M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%* @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%'  +75 h  .tdelay 2 seconds +200 P%j% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%<' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%5 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%w  +38 h  .tdelay 2 seconds +200 P%p% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %s'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P% % h %r .t\PS.seek 0.475ml P%T' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%y  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%C  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%r  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%U @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%h  +76 h  .t\PS.seek 2.5ml P%Y' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%x +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%2' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%; +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%o  +75 h P%6% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %"' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%f% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %\ 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'+ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %J' +1313 h ' .t\PSV.dial :air P%K  +37 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :PML P%M +38 h .t\PS.seek 0ml P%Z' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'l& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'A& F*) @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*k  P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%$% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%=' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +128 h I F*v% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *!'6 +66 .t\PSV.dial :waste1 P%G  +38 h  .t\PS.seek 0ml P%#' +1597 h ' .t\PSV.dial PSR P%S +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%~ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*P  +22 h ' .t\PSV.dial PSR P%N +21 h  F*i% h %6 ."MFBPCR"\AS.seek 0.329ml F*}'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%9' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*` % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%-  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%I +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*7 I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*:' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial PSR P%D +113 h P%b% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%.  +37 h  .t\PS.seek 0ml P%@' +740 h ' ."MFBPCR"delay 5 seconds +500 F*39 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*t n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*R'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Q' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial PSR P%E +113 h P%%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +38 h  .t\PS.seek 0ml P%&' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%8 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %0'k +2678 h 'k .t\PSV.dial :waste1 P%>  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %{' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*_ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *['V +850 h ' .t\PSV.dial :air P%B  +113 h  .t\PS.seek 2.5ml P%,' +2609 h ' .t\PSV.dial :mixloop P%g +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %a' +5107 h ' .t\PSV.dial :air P%F  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %H' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*X  +43 h  F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*e I +43 h I F*^% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%| +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F** n +129 h n ."MFBPCR"\AS.seek :empty F*u'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +85 h  F*'% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *j' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'5% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'p' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'<  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C's'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*y  +21 h ."MFBPCR"delay 3 seconds +300 F*T% h %_ ."MFBPCR"\AS.seek 0.434ml F*C'ݏ +110 h '4 .t\CSV.dial CSR C'r  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*U  +21 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*h'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*x% h %& ."MFBPCR"\AS.seek 0.092ml F*Y'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C';'4 +2648 h '4 C'o& h &W #tGaraging FlushPuck in Collection Clamp M&6& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&+/ +150 h / .t\CC.seek :open C'\ ( +416 h ( M&K& h &ӷ M&J% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &n(- +264 h (- M&l& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M& 1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'L& h & M&v% h %\  A(G% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (=(,% +201 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (S& h &,% M&#& h &- M&~% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +165 h (k M&P& h &k A(i& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &N'Ю +90 h 'Ю A(& h &,% ."WCR"\SE.seek 14.71 Pucks below Top Plate A(!'] +116 h '] A(/& h &,% M&`& h &k ."WCR"\Elbow.seek 4 M&}(k +6 h (k M&-& h &k A(& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&]' +23 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &9/ +138 h '&0 ."MFBPCR"delay 3 seconds +12 h / ."downElevator"\SE.seek :down A(4'X M&I& h &k M&7% h % ."WCR"\Forearm.seek :retract M&'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +55 ."MFBPCR"\ATV.dial :waste2 F*D % +36 h ' ."WCR"\CC.seek :closed C'b(W +7 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *?'R +409 h (W ."WCR"Hand.open M&.1 +150 h 1 M&1% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ + h 'R ."MFBPCR"\ATV.dial :air2 F*t  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +69 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S):+ +6 h + S)% h % C'@& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X  +43 h  #"MFBPCR"\Swish #1 of 2 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *_'\  +1031 h '\  F*q% h %\  ."MFBPCR"\AS.seek 0.5ml F*B'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*{% h %(A ."MFBPCR"\AS.seek 0.6ml F*g'\  +1031 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*a'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*X% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *W' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*[ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +3472 h 'V ."MFBPCR"\SPE.coast F*F  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1509h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*^  +64 h  ."MFBPCR"\ARV.dial :carrier F*|  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*j  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*w  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.53ml F*V' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +107 h  ."MFBPCR"delay 7 seconds +700 F*r% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 F*h  +64 h ."MFBPCR"\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254581 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'o @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '+' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'c @ +75 h @ +50 S)f% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'K% h % ."WCR"\CSV.dial between air and CTV C'\\ ` +19 h ` ."WCR"\CS.seek 7.288ml C'"' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'J  'l  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '2'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'A' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'M  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'$  +38 h  C'v% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'L @ +76 h @ +50 S)G% h % ."WCR"\CBV.dial between CSV and waste2b C'~  +94 h  C'k% h % ."WCR"\CSV.dial between air and CTV C'# ` +56 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'P  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '/'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'`  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'n' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Z  +37 h  C'-% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'9' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)N% h % ."WCR"\CBV.dial between CSV and waste2b C'7  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'] ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'D  '}  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'!  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '\ ' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'Y  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&1% h %\  A(t% h %X A(.. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (@(~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&& h &ӷ M&?% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &=( +143 h ( M&& h & A(%& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &E'н +89 h 'н A(:& h &~ ."WCR"\SE.seek 4.71 Pucks below Top Plate A((' +215 h ' M&d& h & A(O& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &R' +25 h ' ."WCR"\Elbow.hold M&&, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(z- M&$&.,(\ #b h -+ ."WCR"\SE.seek 5.04 Pucks below Top Plate A(>' +27 h ' M&8% h % A(& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&q'- +21 h '- M&_& h & A({& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &B'н +22 h 'н ."WCR"\SE.seek 6.04 Pucks below Top Plate A(0' +36 h ' M&g& h & M&X% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  ."WCR"\Elbow.seek CC M&[(ӷ +143 h (ӷ M&W& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ ."WCR"\Forearm.seek CC M&^' +91 h ' ."WCR"Hand.close M&|/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(,& h &~ A(H& h &~ M&& h &ӷ M&*% h % ."WCR"\Forearm.seek :retract M& '\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &e( +142 h ( M&& h & A( & h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &'' +93 h ' ."WCR"\Elbow.hold M&j, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &51 +150 h 1 M&% h % A(w& h &~ ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &<'- +20 h '- M&& h & A( & h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &p'н +23 h 'н A(V% h % ."downElevator"\SE.seek :down A(T'X +100 (r% h % M&% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +89 h '\  +12 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C's& h & .tHand.open M&1 +2 A(6 h 1 M&& h & M&U% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.seek :garage M&x'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'f(W +416 h (W C'& h &W .tHand.open M&K1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'l& h &W C'J% h % .t\CSV.dial CSR C'"  +38 h  C' % h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'2'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'M% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '$'[ +463 h '[ .t\CSV.dial :waste C'v +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'A'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'L% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '~'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'#% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'P  '  +37 h  +113  .t\CBV.dial :waste1 C'i @ +76 h @ .t\CS.seek 0ml C'`'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'/& h &W C'% h %4 .t\CSV.dial :flush C'Z  +76 h  .t\CS.seek 5ml C'-'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +38 h @ C'9% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'N  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'7  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C']& h &W C'I% h %4 .t\CSV.dial :flush C'}  +75 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'!% h %p .t\CS.seek 7ml C'4'i +416 h 'i ."connect"\CSV.dial CBV C'Y .t\CBV.dial CSV C'  +37 h +76 .t\CTV.dial :air C'1  +150 h .t\CS.seek 0ml C't'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'.'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'?& h &W C'% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.seek 3ml C':'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'3% h %0 .t\CS.seek 5ml C'd'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'O'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +507 h '0 .t\CSV.dial :waste C'8 +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'_& h &W C'{% h %4 .t\CSV.dial CSR C'g  +150 h  C'X% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'E'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'=' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +113 h .t\CTV.dial :intake C'R  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'W' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'F  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'q'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'>  +113 h  C'B% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'&'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  'b  +37 h  +113  .t\CBV.dial :waste1 C', @ +38 h @ .t\CS.seek 0ml C'H'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'*% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV C'^  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'[  +76 h  .t\CS.seek 0ml C'5'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CBV C'V .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'e  +151 h .t\CS.seek 0ml C'r'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C's +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'& h &W C'U% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.seek 5ml C'<'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'C% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'p +113 h .t\CTV.dial :air .\CS.seek 0ml C'T'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +506 h '0 .t\CSV.dial :waste C'j +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'x'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C';% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'K'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'\\& h &W C'l% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 5ml C'"'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'c% h %p .t\CS.seek 7ml C'\ 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  '2  +38 h  +113  .t\CBV.dial :waste1 C'v @ +38 h @ .t\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'L% h %4 .t\CSV.dial :flush C'G  +75 h  .t\CS.seek 5ml C'A'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'#% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'P  +76 h  .t\CS.seek 0ml C'i'4 +766 h '4 #tDisinfecting external Intake with kill C'S& h &W C'/% h %4 .t\CSV.dial CSR C'  +37 h  C'Z% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'`'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'9 '-  +38 h +75 .t\CTV.dial :intake C'N  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'7' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ']'4 +607 h '4 .t\CSV.dial CSR C'I  +113 h  C'}% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1373 h ' .t\CSV.dial CBV C'! +113 h .t\CS.seek 0ml C'D'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +6 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'.( +416 h ( M&?& h &ӷ M&% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &:'η +90 h 'η .tHand.open M&d1 +150 h 1 M&O% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'z& h & .tHand.open M&_1 +2 h 1 M&{& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &g(- +7 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&E'η +91 h 'η .tHand.close M&(/ +150 h / M&R& h &- M&=% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +264 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C'&(W +416 h (W C'a& h &W .tHand.open M&b1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'*% h %4 .t\CSV.dial CSR C'H  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'^'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '[% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'w'4 +2010 h '4 #t\Purging external Intake C'5& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'e +76 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'r'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C's  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'U  +75 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'0% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV C'< .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'p'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'h'0 +506 h '0 .t\CSV.dial :waste C'T +38 h .t\CS.seek 0ml C'j'4 +506 h '4 C'@& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'y'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'o% h %0 .t\CS.seek 5ml C';'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'x'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'6 +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +6 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&K' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'c( +416 h ( M&"& h &ӷ M&M% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &v(- +265 h (- M&\ & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&G1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Completed [mbaPhase] begun 06:04:07 #\Preparing to sleep until Wed Oct 17 06:04:07 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:04CEST17-Oct-18 #\Continuing at 06:04CEST17-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:55:30CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:04CEST17-Oct-18" +235172 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1509h25ml.pcr F*#% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*P % +87 h % F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*Z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +21 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*-'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +21 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +65 h  F*I% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*]' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*4  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*?% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1511h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*E  +42 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*g'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*,  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *H'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*D' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*[  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*s'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*0% h % \\ ."MFBPCR"\AS.seek 0.326ml F*''{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1511h25ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*h  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*l  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*)'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*~% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1513h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ARV.dial :carrier F*}  +10 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *f'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*? I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*+% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*d  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +107 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*{ n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*:  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*E% h % \\ ."MFBPCR"\AS.seek 0.326ml F*>'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1513h25ml.pcr F*g% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*F  +22 h ."MFBPCR"\ATV.dial :air3 F*a % +87 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +21 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +21 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +31 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*<% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*''m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*T  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1516h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*|'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 F*#,,'T0 b ."MFBPCR"\AS.seek 0.506ml F*\ 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*2 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*L% h %Fj ."MFBPCR"\AS.seek 0.524ml F*$' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*# I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*)' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +107 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*S n +21 h n ."MFBPCR"delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.6ml F*`'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*-  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*7% h % \\ ."MFBPCR"\AS.seek 0.326ml F*n'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1516h25ml.pcr F*I% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*4'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*d  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +65 h  F*Y% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*(  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*R% h % ."MFBPCR"\AS.seek 0.81ml F*B'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*.  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct1518h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *e'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*U n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*0' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*Q  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*C' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*h  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.536ml F*''< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*j'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*;  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*6' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct1518h25ml.pcr F*J% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*"  +21 h ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*$'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*~  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b */'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*9  +32 h  ."MFBPCR"\ATV.dial :air3 F*` % +86 h % F*-% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*N% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*n'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*!% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *3'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1520h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*Y  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +10 h  F*O% h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *:'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*E I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*8 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.53ml F*B' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*>'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*.  +21 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F**'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*b n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*H  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*^' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1520h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *s' +424 h ' ."MFBPCR"\ATV.dial :ASV F*r n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*U'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*< n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *Q'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*p'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*0 % +43 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *@' +423 h ' ."MFBPCR"\ATV.dial :air3 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*'% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h % H ."MFBPCR"\AS.seek 0.735ml F*;' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*y% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*l% h % zG ."MFBPCR"\AS.seek 0.365ml F*6' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*J % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*M% h % ."MFBPCR"\AS.seek 0.579ml F*|' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*L% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*K' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*# n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*\ 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *~'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*) % +108 h % F*$% h %V ."MFBPCR"\AS.seek 0.01ml F*/' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*S' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*9'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*-% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*Z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*7 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*}  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*! n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *n'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*1'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*?  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *4' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*d  +43 h  F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*O  +65 h  F*3% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *z'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*{ n +65 h n F*%% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *X' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*( I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*_  +43 h  F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*:  +65 h  F*8% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *g'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*F% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *a' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*B I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *.'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*,  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *>': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*b n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F** n +151 h n ."MFBPCR"\AS.seek 0ml F*H'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*q % +43 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +21 h F*^% h % ."MFBPCR"\AS.seek 0.96ml F* '0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F* ' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*e% h % ."MFBPCR"\AS.seek 0.97ml F*V'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*r I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *<'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*Q  +43 h  F*0% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *p' +9988 h ' ."MFBPCR"\ATV.dial AIV F*C I +43 h I ."MFBPCR"\AS.seek 0.02ml F*@' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*''V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*T% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*j  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*6'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*&  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*\ % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*/'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' @CEST1539640852.52 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *n'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast S)5 F*5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast .\Forearm.coast .\Elbow.coast P%6 M&5 + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539749047.11 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )? h  C' '+ h  P% %1 h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *4  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)d% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'Y& S)#.0X(d +47 h (d +59 / A(*& h &,? .t\SE.reconfigure SEconfig .\SE.home.seek :down A(#K-0-\ b ('X +149 h 'X .t\SC.reconfigure SCsloppy .\SC.home.seek :initial A($-.(\ 1@~b (( +345 h ' +13 (  .t\Forearm.seek :retract M&q'\ +6 h '\  .t\Elbow.seek :hammer M&D(_ + h '4 +92 (_ .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &H' +91 h ' M&5% h % .t\Forearm.seek :retract M&^'\ +91 h '\  M&[% h %\  P%w& h &2 C'& ."openPC"\PC.reconfigure PCconfig .\PC.home.seek :open h &3 P%$x2 ^22b .t\CC.reconfigure CCconfig P% ( .t\CC.home.seek :open C'$x2 ^22b ' ( +222 h ( +3 ( +160 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 06:04:22 began in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:56:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #18) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M& 1 +150 h 1 M&r& h &_ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &<'η +91 h 'η .tHand.close M&U/ +150 h / M&Q& h &- M&0% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +265 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'@& h &W .tHand.open M&'1 +150 h 1 M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'o& h &W C'% h %4 .t\CSV.dial CSR C';  +151 h  C'x% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'y'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'l +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +2010 h '4 #t\Purging external Intake C' & h &W C'6% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W  '" h +76 .t\CTV.dial :intake C'\\  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'G'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'|  +76 h #t\Flushing upper manifold C'2& h &W C'L% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'\ % h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C') +113 h .t\CTV.dial :air .\CS.seek 0ml C'$'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +506 h '0 .t\CSV.dial :waste C'A +38 h .t\CS.seek 0ml C''4 +506 h '4 C'~& h &W C'i% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'/'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'S% h %0 .t\CS.seek 5ml C'-'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'9'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'N +75 h .t\CS.seek 0ml C'Z'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +7 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&!' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:56:32CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:56:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*c% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&?% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(+% h %X A(1. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +6 F*4% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *d' +350 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (Y& h &,% M&f& h &- M&O% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &3(k +166 h (k M&t& h &k A({& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &%'Ю +89 h 'Ю A(z& h &,% .t\SE.seek 13.71 Pucks below Top Plate A(('n +126 h 'n A(X& h &,% M&& h &k .t\Elbow.seek 4 M&_(k +6 h (k M&E& h &k A(:& h &,% .t\Forearm.seek 4 + 300 counts M&8' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(='X M&F& h &k M&g% h % .t\Forearm.seek :retract M&a'\ +90 h '\  .t\Elbow.seek CC M&*(ӷ +35 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &[' +91 h ' .t\CC.seek :closed C'w(W +416 h (W .tHand.open M&1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)q+ +6 h + S)H% h % C'e& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'L9 h 9Td +100 C'\\9 h 9kd +100 C'9 h 9d +100 C'G9 h 9d +100 C'\ 9 h 9d +100 C' 9 h 9d +100 C')9 h 9d +100 C'#9 h 9d +100 C'$9 h 9 d +100 C'J9 h 9 d +100 C'29 h 96d +100 C'P9 h 9Ld +100 C'~9 h 9bd +100 C'i9 h 9xd +100 C'K9 h 9d +100 C'9 h 9d +100 C'`9 h 9d +100 C'S9 h 9d +100 C'9 h 9d +100 C'/9 h 9d +100 C'99 h 9d +100 C'N9 h 9(d +100 C'9 h 9>d +100 C'-9 h 9Td +100 C'I9 h 9id +100 C'A9 h 9d +100 C'}9 h 9d +100 C'79 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'T9 h 9d +100 C'9 h 9  d +100 C'v9 h 9 d +100 C'h9 h 9 *d +100 C'!9 h 9 ?d +100 C'k9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C']9 h 9 (d +100 C'n9 h 9 ,d +100 C'c9 h 9 0d +100 C'?9 h 9 4d +100 C'+9 h 9 8d +100 C'19 h 9 & h &,% M&M& h &k .t\Elbow.seek 4 M&L(k +7 h (k M&\\& h &k A(G& h &,% .t\Forearm.seek 4 + 300 counts M&\ ' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &#/ +150 h / ."downElevator"\SE.seek :down M& & A($'X h &k M&;% h % .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.seek CC M&"(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W .tHand.open M& 1 +150 h 1 M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'J  +151 h  .t\CSV.dial CTV C'w  +18 h  .t\CS.seek 5ml C'`'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'S' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%/  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%- +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%i  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%2 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%}  +151 h  .t\PS.seek :empty P%7' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'T  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'h  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'9 ` +19 h ` .t\CSV.dial :air C'N @ +38 h @ .t\CS.seek 10ml C'k' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C']'4 +2648 h '4 .t\CBV.dial :waste1 C'c @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'?  +150 h C'+& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&1% h %\  A(4% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (I(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&)& h &ӷ M&v% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&Y& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &!'н +89 h 'н A(@& h &~ .t\SE.seek 6.71 Pucks below Top Plate A(A' +196 h ' M&& h & A(t& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &{' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(f- h -+ M&$&.,(\ #b .t\SE.seek 7.04 Pucks below Top Plate A(' +27 h ' M&% h % A(& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &z'- +21 h '- M&& h & A(X& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н .t\SE.seek 8.04 Pucks below Top Plate A(m'w +36 h 'w M&:& h & M&F% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +90 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C'(( +416 h ( A(_& h &~ A(a& h &~ M&,& h &ӷ M&% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &H( +142 h ( M&& h & A(R& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &e' +93 h ' .t\Elbow.hold M&V, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &b1 +150 h 1 M&% h % A(s& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &D'- +21 h '- M& & h & A(O& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н A(u% h %w ."downElevator"\SE.seek :down A(0'X +100 (^% h % M&d% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +81 h 'X ."downElevator"\SE.coast A(5 +8 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +113 h  P%[% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%r +38 h .t\PRV.dial :air P%B  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%'  +38 h .t\CBV.dial AIV C'j  +37 h .t\PRV.dial between CBV and air P%o  +19 h  .t\PRV.dial :air P%  +19 h  P%.% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&61 +2 h 1 M&y& h & M&Q% h %\  .t\Elbow.seek FlushPuck::Garage M&>(- +165 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&G'η +91 h 'η .tHand.close M&U/ +150 h / M&#& h &- M& % h %η .t\Forearm.seek :retract M&;'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +265 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C'\ (W +416 h (W C'W& h &W .tHand.open M&L1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%|  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%J  +75 h  .tdelay 2 seconds +200 P%w% h % .t\PS.seek 1.499ml P%' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%x' +1094 h ' .t\PSV.dial :air P%n  +37 h  .t\PS.seek 0ml P%`' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%S +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%/  +37 h  .tdelay 2 seconds +200 P%q% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %-'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%i  +75 h .tdelay 2 seconds +200 P%2% h %r .t\PS.seek 0.475ml P%}' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%T  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %h' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%9 +75 h .t\PTV.dial :PRV,avoiding: :puck P%N @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%k' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%c +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%?' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%+  +75 h  .t\PS.seek 2.5ml P%1' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%4 +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %)' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'v & +50 h & .t\AWV.select :waste C' +6 P%Y% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %"' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%t' +2610 h ' .t\PSV.dial :PML P%K +37 h .t\PS.seek 0ml P%f' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *X  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%: +22 h  ."MFBPCR"delay 20 seconds +91 h P%F% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%!' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*A% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *{'6 +66 .t\PSV.dial :waste1 P%3  +37 h  .t\PS.seek 0ml P%5' +1598 h ' .t\PSV.dial PSR P%z +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*8  +22 h ' .t\PSV.dial PSR P%E +21 h  F*Z% h %6 ."MFBPCR"\AS.seek 0.329ml F*_'ͯ +92 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%,' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%R  +38 h  .t\PS.seek 0ml P%g' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*%  +22 h  F*b% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%s +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +217 ."MFBPCR"\ATV.dial :AIV F*O I +43 h I F*u% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%^' +2610 h ' .t\PSV.dial PSR P%d +112 h P%e% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*[9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*0% h % ."MFBPCR"\AS.seek 0.041ml F*I'% +336 h ' .t\PSV.dial PSR P%7 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%r' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%B +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%'' +2610 h ' .t\PSV.dial PSR P%j +113 h P%o% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%.  +37 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%6 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %y'k +2679 h 'k .t\PSV.dial :waste1 P%Q  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %M' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*\\ I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *V'V +849 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%#' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %p' +5107 h ' .t\PSV.dial :air P%G  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %*' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*P  +43 h  F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *;'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*( I +42 h I F*W% h %Q ."MFBPCR"\AS.seek 0.01ml F*L' +171 h ' .t\PSV.dial :mixloop P%$ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*~'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*|  +86 h  F*J% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *w' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *n'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'x  +37 h  C'S% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'/'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  'i  +38 h  +113  .t\CBV.dial :waste1 C'2 @ +37 h @ .t\CS.seek 0ml C'-'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*}% h %_ ."MFBPCR"\AS.seek 0.434ml F*T'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'9'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*N  +22 h F*h% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*c% h %& ."MFBPCR"\AS.seek 0.092ml F*k'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'+' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'4'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +7 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +415 h ( M&& h &ӷ M&"% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &X'η +91 h 'η .tHand.open M&:1 +150 h 1 M&F% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'& h & M&A% h %\  A(3% h %X A(<. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (!(,% +200 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (z& h &,% M&5& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +166 h (k M&8& h &k A(Z& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &E'Ю +89 h 'Ю A(a& h &,% ."WCR"\SE.seek 11.71 Pucks below Top Plate A({'Ɛ +146 h 'Ɛ A(& h &,% M&& h &k ."WCR"\Elbow.seek 4 M&_(k +6 h (k M&R& h &k A(%& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&b' +23 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &,/ +108 h '&0 ."MFBPCR"delay 3 seconds +42 h / ."downElevator"\SE.seek :down M&& A(s'X h &k M&O% h % ."WCR"\Forearm.seek :retract M&u'\ +91 h '\  ."WCR"\Elbow.seek CC M&g(ӷ +54 h 'X ."downElevator"\SE.coast A(5 +88 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +25 ."MFBPCR"\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +23 h ' ."WCR"\CC.seek :closed C'^(W +416 h (W ."WCR"Hand.open M&m1 +121 h 'R ."MFBPCR"\ATV.dial :air2 F*D  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *[' +7 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +6 h + S)7% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'B  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +76 h  #"WCR"\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'V% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*V % +42 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +3473 h 'V ."MFBPCR"\SPE.coast F*G  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1709h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*W  +64 h  ."MFBPCR"\ARV.dial :carrier F*$  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *;'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*w  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*S% h %Fj ."MFBPCR"\AS.seek 0.524ml F*x' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*/  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*2 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.536ml F*i'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*}  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*T  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*c  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*+'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254610 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'v  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'l @ +75 h @ +50 S)Y% h % ."WCR"\CBV.dial between CSV and waste2b C'\  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'& ` +19 h ` ."WCR"\CS.seek 7.288ml C')' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'"  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '1'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C':  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'F  +37 h  C'A% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'<' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)3% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'X% h % ."WCR"\CSV.dial between air and CTV C'5 ` +56 h ` ."WCR"\CS.seek 7.288ml C'Z' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'a  '8  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 't' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'%  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +38 h  C'R% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C',' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)E% h % ."WCR"\CBV.dial between CSV and waste2b C'O  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C'{ ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'd  '_  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 's'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'b  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'u& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&m% h %\  A(% h %X A(D. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (B& h &~ M&7& h &ӷ M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &!( +143 h ( M&e& h & A(o& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &j'н +89 h 'н A(=& h &~ ."WCR"\SE.seek 7.71 Pucks below Top Plate A(.' +185 h ' M&'& h & A(& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &I' +25 h ' ."WCR"\Elbow.hold M& , +7 h , ."WCR"\Elbow.reconfigure TightElbow A(6- h -+ M&$&.,(\ #b ."WCR"\SE.seek 8.04 Pucks below Top Plate A(Q'w +27 h 'w M&% h % A([& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&'- +21 h '- M&\\& h & A(M& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &U'н +22 h 'н ."WCR"\SE.seek 9.04 Pucks below Top Plate A(y'f +36 h 'f M& & h & M&P% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +90 h '\  ."WCR"\Elbow.seek CC M&V(ӷ +142 h (ӷ M&>& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ ."WCR"\Forearm.seek CC M&W' +91 h ' ."WCR"Hand.close M&$/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(#& h &~ A(*& h &~ M&L& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&|'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &(( +143 h ( M&C& h & A(& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &J' +92 h ' ."WCR"\Elbow.hold M&w, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &S1 +150 h 1 M&;% h % A(/& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&`'- +21 h '- M&x& h & A(2& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &q'н +22 h 'н A(]% h %f ."downElevator"\SE.seek :down A(}'X +100 (% h % M&i% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +72 h 'X ."downElevator"\SE.coast A(5 +17 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'-& h & .tHand.open M&1 +2 h 1 M&h& h & M&N% h %\  .t\Elbow.seek FlushPuck::Garage M&n(- +166 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&?/ +150 h / M&& h &- M&9% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'Y(W +415 h (W C'\ & h &W .tHand.open M&1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'"% h % .t\CSV.dial CSR C')  +38 h  C':% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'1'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'K% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'F'[ +463 h '[ .t\CSV.dial :waste C'A +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'<& h &W C'% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'5% h %p .t\CS.seek 7ml C'z'i +416 h 'i ."connect"\CSV.dial CTV C'a  .t\CTV.dial CSV C'8  +37 h  +113  .t\CBV.dial :waste1 C'Z @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'%% h %4 .t\CSV.dial :flush C'f  +76 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C',% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'O  +76 h  .t\CS.seek 0ml C'H'4 +765 h '4 #t\Flushing upper manifold C'{& h &W C'% h %4 .t\CSV.dial :flush C'_  +76 h  .t\CS.seek 5ml C'd'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C's'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'u  +37 h +76 .t\CTV.dial :air C'm  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'B +37 h .t\CS.seek 0ml C'7'4 +507 h '4 C' & h &W C'e% h %4 .t\CSV.dial :flush C'o  +38 h  .t\CS.seek 3ml C'='0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'g @ +38 h @ C''% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'0'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'6'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'['4 +506 h '4 #tDisinfecting external Intake with mfbkill C'\\& h &W C'M% h %4 .t\CSV.dial CSR C'  +151 h  C'P% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'j'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'. +113 h .t\CTV.dial :intake C'I  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'>' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'G  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'Q  +113 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '$' +710 h ' ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV C'^  +37 h  +113  .t\CBV.dial :waste1 C'# @ +38 h @ .t\CS.seek 0ml C'*'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'L  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'C'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  +38 h  .t\CBV.dial :waste2b C'V  +75 h  .t\CS.seek 0ml C'S'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C';  +38 h  C'/% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'x'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C']  '| +38 h +75 .t\CTV.dial :air C'(  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'i'0 +507 h '0 .t\CSV.dial :waste C'- +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'h& h &W C'N% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 5ml C'`'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +38 h @ C'T% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'q +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'w +38 h .t\CS.seek 0ml C'?'4 +506 h '4 C'& h &W C'9% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'c'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'\ +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'&& h &W C'% h %4 .t\CSV.dial :flush C'"  +37 h  .t\CS.seek 5ml C')'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C': @ +37 h @ C'l% h %p .t\CS.seek 7ml C'k'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'1  'K  +38 h  +113  .t\CBV.dial :waste1 C'A @ +38 h @ .t\CS.seek 0ml C'F'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'<& h &W C'% h %4 .t\CSV.dial :flush C'3  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'5% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  +37 h  .t\CBV.dial :waste2b C'8  +76 h  .t\CS.seek 0ml C'Z'4 +766 h '4 #tDisinfecting external Intake with kill C'z& h &W C'% h %4 .t\CSV.dial CSR C'%  +37 h  C'f% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C't' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R ',  +37 h +76 .t\CTV.dial :intake C'E  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'O' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '{'4 +607 h '4 .t\CSV.dial CSR C'  +112 h  C'_% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1373 h ' .t\CSV.dial CBV C'b +112 h .t\CS.seek 0ml C'd'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&u& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&B/ +150 h / .t\CC.seek :open C'D( +416 h ( M& & h &ӷ M&e% h % .t\Forearm.seek :retract M&o'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &7(- +264 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'6& h & .tHand.open M&\\1 +2 h 1 M&M& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&./ +150 h / M&I& h &- M&!% h %η .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &>(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'p& h &W .tHand.open M&^1 +150 h 1 M&#% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'L& h &W C' % h %4 .t\CSV.dial CSR C'*  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'V% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'; +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '/'4 +2010 h '4 #t\Purging external Intake C'S& h &W C'x% h %4 .t\CSV.dial :flush C']  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +75 h .t\CTV.dial :intake C'2  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'-  +75 h #t\Flushing upper manifold C'i& h &W C'h% h %4 .t\CSV.dial :flush C'N  +76 h  .t\CS.seek 5ml C'J'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'y% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CBV C'` .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'} +38 h .t\CS.seek 0ml C'w'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +38 h @ C'% h %0 .t\CS.seek 5ml C'4'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'\ '4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &&(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&:/ +150 h / .t\CC.seek :open C'l( +416 h ( M&)& h &ӷ M&K% h % .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +265 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&31 +150 h 1 M&F% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Completed [mbaPhase] begun 06:04:22 #\Preparing to sleep until Fri Oct 19 06:04:22 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:04CEST19-Oct-18 #\Continuing at 06:04CEST19-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:57:59CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:04CEST19-Oct-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1709h25ml.pcr F*5% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*a  +22 h ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*f'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*,'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*O  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*B % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*e'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*''m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*Q  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*#  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*C  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*V  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*W% h % ."MFBPCR"\AS.seek 0.53ml F*;' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*x'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +107 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*('\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F*-'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1711h25ml.pcr F*q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *9'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +21 h F*?% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +65 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*<  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *%'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F*{% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*v  +22 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*g n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*o'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*0  +108 h  ."MFBPCR"delay 7 seconds +700 F*6% h %< ."MFBPCR"\AS.seek 0.594ml F*\\'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*M n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.65ml F*I' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*7  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*j% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1713h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*G  +21 h ."MFBPCR"\ATV.dial :air3 F*p % +87 h % F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *L'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*V'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*d% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*S  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +65 h  F*]% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b */'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*T'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*}  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*>% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*) n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *k'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*1 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*5 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*8 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*z n +22 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.65ml F*E' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*,  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*O% h % \\ ."MFBPCR"\AS.seek 0.326ml F*t'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*{ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1716h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*b % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*m% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*e% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*.  +32 h  ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*!% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*U'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*#'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct1718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*/% h %V ."MFBPCR"\AS.seek 0.5ml F*x'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*-  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*N n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*r  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*T' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*}  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*? n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*c  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*> % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct1718h25ml.pcr F*"% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*)  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*3% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*8  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*H  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*0% h %V ."MFBPCR"\AS.seek 0.5ml F*6'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *P'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*.  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*[  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *='Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*j I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*7' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*U' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +22 h  ."MFBPCR"delay 7 seconds +700 F*#% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*d  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*S% h % \\ ."MFBPCR"\AS.seek 0.326ml F*]'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*/ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*; @@T ."MFBPCR"\ASV.dial :SPEdelivery F*x  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*2  +151 h F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *-' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*i% h % ."MFBPCR"\AS.seek 0.62ml F*N'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *r'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*y % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*T% h % ."MFBPCR"\AS.seek 0.53ml F*}' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*J% h % ."MFBPCR"\AS.seek 0.71ml F*?' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*w% h % H ."MFBPCR"\AS.seek 0.735ml F*4' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*n' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*c n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*" % +43 h % F*:% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*$' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h %  ."MFBPCR"\AS.seek 0.604ml F*<'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*5 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*+  +22 h  ."MFBPCR"\AS.seek 0ml F*k'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*F% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*a% h % ."MFBPCR"\AS.seek 0.58ml F*z' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h % q ."MFBPCR"\AS.seek 0.605ml F*R'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*,% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*f' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*O n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*E  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*_  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*b n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *t'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*H n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +202 h 'R ."MFBPCR"\SPE.coast F*v  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*m'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*B n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *s' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*g I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*0  +65 h  F*'% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *6'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*M n +65 h n F*o% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *P' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*. I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\\  +42 h  F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *I': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*=  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*7 n +65 h n F*G% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *p' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*U I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*#  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*^ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *L'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F**'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*W% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %0 ."MFBPCR"\AS.seek 0.985ml F*]' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*(% h % ."MFBPCR"\AS.seek 0.97ml F*|'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *i'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *`'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*r  +43 h  F*y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *q' +9988 h ' ."MFBPCR"\ATV.dial AIV F*T I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*J'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*}% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +21 h ."MFBPCR"\ATV.dial :air3 F*? % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*c% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *&'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*%% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V @CEST1539813604.69 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *t'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*u % +108 h % ."MFBPCR"\AS.seek 0ml F*B'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast F*5 C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 &6 C'5 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539921862.55 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )  h  C' '@ h  P% %m h  F* *g h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *s  h  .t\SPE.configure SPEconfig F*\ 2 *e% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:58:56CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #19) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'(& h & .tHand.open M&]1 +150 h 1 M&& h &_ M&|% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &`'η +91 h 'η .tHand.close M&N/ +150 h / M&r& h &- M&y% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'}(W +416 h (W C'& h &W .tHand.open M&J1 +150 h 1 M&w% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'9% h %4 .t\CSV.dial CSR C'4  +150 h  C'c% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '"'4 +2009 h '4 #t\Purging external Intake C':& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C') .t\CBV.dial CSV C'>  h +75 .t\CTV.dial :intake C'&  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'3'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'$  +75 h #t\Flushing upper manifold C'1& h &W C'% h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C'+'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'k% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'F'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'& h &W C'Z% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'z% h %0 .t\CS.seek 5ml C','p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'R'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'E +75 h .t\CS.seek 0ml C'f'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&b' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&H& h &ӷ M&u% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&A& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &l'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:59:00CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:58:56CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C';& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M& % h %\  A(@% h %X A(m. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (g(,% +6 F*s% h %&K ."readyMFB"\ATV.dial :waste2 F*e % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +351 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M&Y& h &- M&0% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &'(k +166 h (k M&& h &k A(M& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &o'Ю +89 h 'Ю A(6& h &,% .t\SE.seek 10.71 Pucks below Top Plate A(.' +155 h ' A(P& h &,% M&[& h &k .t\Elbow.seek 4 M&\\(k +6 h (k M&j& h &k A(=& h &,% .t\Forearm.seek 4 + 300 counts M&!' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &7/ +150 h / ."downElevator"\SE.seek :down A(G'X M&& h &k M& % h % .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +64 h 'X ."downElevator"\SE.coast A(5 +78 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' .t\CC.seek :closed C'/(W +416 h (W .tHand.open M&x1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)*% h % C'(& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W ."airStroke"\CSV.dial :waste S))% h % ."airStroke"\CS.seek 0ml ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV C'&'4 S)K  +7 h '4 ."airStroke"\CSV.dial :air C'$ @ +75 h @ ."airStroke"\CS.seek 10ml C'1' +144 h  .t\CTV.dial :air C'  +76 h .tdelay 15 seconds +690 h ' +810 .t\SSV.dial :spit,avoiding: :exhaust S)< +150 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'3  .t\SS.seek 0ml S)' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)k  +144 h  +6  S)+% h % .t\CBV.dial between SSV and waste2 C'  +19 h  C'5% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'F  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +2610 h '4 C'8% h %4 S)% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'Z @ +18 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X9 h 9|d +100 C')9 h 9d +100 C'$9 h 9d +100 C'K9 h 9'd +100 C'f9 h 9>d +100 C'9 h 9Td +100 C'&9 h 9kd +100 C'<9 h 9d +100 C'39 h 9d +100 C'k9 h 9d +100 C'+9 h 9d +100 C'9 h 9d +100 C'59 h 9d +100 C'F9 h 9 d +100 C'"9 h 9 d +100 C'19 h 96d +100 C'89 h 9Ld +100 C'9 h 9bd +100 C'Z9 h 9xd +100 C'9 h 9d +100 C'a9 h 9d +100 C'9 h 9d +100 C'z9 h 9d +100 C'%9 h 9d +100 C'9 h 9d +100 C'R9 h 9d +100 C'E9 h 9(d +100 C'9 h 9>d +100 C',9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'_9 h 9d +100 C'O9 h 9d +100 C'9 h 9d +100 C'?9 h 9d +100 C'}9 h 9d +100 C'u9 h 9  d +100 C'A9 h 9 d +100 C'9 h 9 *d +100 C'b9 h 9 ?d +100 C'X9 h 9 Td +100 C' 9 h 9 j d +100 C'H9 h 9 $d +100 C'{9 h 9 (d +100 C't9 h 9 ,d +100 C'~9 h 9 0d +100 C' 9 h 9 4d +100 C'@9 h 9 8d +100 C'm9 h 9 & h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &)' +25 h ' .t\Elbow.hold M&$, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(K- h -+ .t\SE.seek 9.04 Pucks below Top Plate A(f'f +27 h 'f M&% h % A(&& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &<'- +21 h '- M&3& h & A(k& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &+'н +22 h 'н .t\SE.seek 10.04 Pucks below Top Plate A('U +36 h 'U M&5& h & M&F% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +90 h '\  .t\Elbow.seek CC M&1(ӷ +142 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&Z' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'a( +416 h ( A(& h &~ A(z& h &~ M&%& h &ӷ M&% h % .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &E( +142 h ( M&& h & A(,& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &_1 +150 h 1 M&O% h % A(& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &?'- +21 h '- M&}& h & A(u& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &A'н +22 h 'н A(% h %U ."downElevator"\SE.seek :down A(b'X +100 (X% h % M& % h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +61 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'{ @ +19 h @ .t\CS.seek 10ml C't' +7 h ' .t\CSV.dial between air and CTV C'~ ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'@& h & .tHand.open M&m1 +2 h 1 M&s& h & M&e% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +165 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&Y'η +91 h 'η .tHand.close M&;/ +150 h / M&& h &- M&M% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +265 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &j' +91 h ' .t\CC.seek :closed C'\ (W +416 h (W C'P& h &W .tHand.open M&71 +150 h 1 M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%= +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%'' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%o  +37 h  .t\PS.seek 0ml P% ' +1597 h ' .t\PSV.dial PSR P%S +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%#' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%!  C'.  h +75 .t\PSV.dial :mixloop P%\\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  h .t\PS.seek 0ml P%G' +2610 h ' .t\PSV.dial :air P%x  +38 h  .t\PS.seek 2.5ml P%v' +2609 h ' .t\PSV.dial :mixloop P%* +38 h .t\PS.seek 0ml P%(' +2610 h ' .t\PSV.dial :air P%I  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P%| +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&g& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +7 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'i( +416 h ( M&y& h &ӷ M&W% h % .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &T'η +91 h 'η .tHand.open M&21 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'J& h & M&w% h %\  A(% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (c(,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (^& h &,% M&n& h &- M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +166 h (k M&& h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & 'Ю +90 h 'Ю A(& h &,% .t\SE.seek 9.71 Pucks below Top Plate A(>' +166 h ' A(K& h &,% M&Q& h &k .t\Elbow.seek 4 M&(k +6 h (k M&&& h &k A(3& h &,% .t\Forearm.seek 4 + 300 counts M&k' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &5/ +150 h / ."downElevator"\SE.seek :down A(:'X M&F& h &k M&4% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M&)(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'f(W +416 h (W .tHand.open M&+1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'"  +150 h  .t\CSV.dial CTV C'/  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'z' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'%'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%, +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%Z  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%1 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%_  +150 h  .t\PS.seek :empty P%O' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'}  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'u @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'R ` +19 h ` .t\CSV.dial :air C'E @ +37 h @ .t\CS.seek 10ml C'X' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'{'4 +2647 h '4 .t\CBV.dial :waste1 C'~ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'@& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&m% h %\  A(s% h %X A(e. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (?& h &~ M&& h &ӷ M&A% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &C( +142 h ( M&& h & A(b& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A(& h &~ .t\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' M&;& h & A(& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &M' +25 h ' .t\Elbow.hold M&H, +7 h , .t\Elbow.reconfigure TightElbow A(Y- h -+ M&$&.,(\ #b .t\SE.seek 10.04 Pucks below Top Plate A(B'U +26 h 'U M&[% h % A(& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&6'- +21 h '- M&a& h & A(P& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &7'н +22 h 'н .t\SE.seek 11.04 Pucks below Top Plate A(l'D +36 h 'D M&=& h & M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +89 h '\  .t\Elbow.seek CC M&o(ӷ +143 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'.( +416 h ( A(\\& h &~ A(p& h &~ M&#& h &ӷ M&x% h % .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &*( +142 h ( M&v& h & A(I& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &(' +93 h ' .t\Elbow.hold M&|, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &D1 +150 h 1 M&g% h % A(-& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &d'- +21 h '- M&]& h & A(0& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &L'н +22 h 'н A(\ % h %D ."downElevator"\SE.seek :down A(y'X +100 (W% h % M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +52 h 'X ."downElevator"\SE.coast A(5 +37 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%V% h % .t\PS.seek 0.499ml P%2' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%U  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%J  +38 h .t\CBV.dial AIV C'w  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%h  +19 h  P%^% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %n' +250 h ' .t\PRV.dial :waste2 P%9 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %T' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&r% h %\  .t\Elbow.seek FlushPuck::Garage M&K(- +166 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &- .t\Forearm.seek :garage M&3'η +91 h 'η .tHand.close M&N/ +150 h / M&5& h &- M&F% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'k(W +415 h (W C'>& h &W .tHand.open M&1 +150 h 1 M&:% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'i& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%+ @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%$  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%"  +75 h  .tdelay 2 seconds +200 P%/% h % .t\PS.seek 1.499ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%c' +1095 h ' .t\PSV.dial :air P%t  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%z +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %,'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%Z  +75 h .tdelay 2 seconds +200 P%1% h %r .t\PS.seek 0.475ml P%_' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%}  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%u  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%R +76 h .t\PTV.dial :PRV,avoiding: :puck P%E @ +75 h @ .t\PS.seek 0ml P%%' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%X' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%~ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%@  +75 h  .t\PS.seek 2.5ml P%m' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%s +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%e  +76 h P%?% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'A & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %)' +1313 h ' .t\PSV.dial :air P%;  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P%Y +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '[& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*a @@T .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*P  P%= +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*C I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *M'6 +66 .t\PSV.dial :waste1 P%'  +37 h  .t\PS.seek 0ml P%S' +1597 h ' .t\PSV.dial PSR P%6 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%B' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%H' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*!  +22 h ' .t\PSV.dial PSR P%j +21 h  F*f% h %6 ."MFBPCR"\AS.seek 0.329ml F*\\'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%#' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*x % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P% ' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*o  +22 h  F*D% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *g' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%- +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%]' +217 ."MFBPCR"\ATV.dial :AIV F*0 I +43 h I F*\ % h % ."MFBPCR"\AS.seek 0.469ml F*G' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%* +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%W' +2609 h ' .t\PSV.dial PSR P% +113 h P%(% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%d' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%l  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*V9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*L n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%O +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +2610 h ' .t\PSV.dial PSR P%w +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%h' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%^  +38 h  .t\PS.seek 0ml P%2' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%9 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%n' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%r  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %Q' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*& I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *|'V +849 h ' .t\PSV.dial :air P%N  +113 h  .t\PS.seek 2.5ml P%5' +2610 h ' .t\PSV.dial :mixloop P%F +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %q' +5107 h ' .t\PSV.dial :air P%3  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*8  +43 h  F*K% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *4'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*. I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%: +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %i' +618 h ' ."MFBPCR"\ATV.dial :waste F*+ n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*$  +86 h  F*"% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b */' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*T  +43 h F*<% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'c  +38 h  C'z% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'Z  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C','4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*_% h %_ ."MFBPCR"\AS.seek 0.434ml F*}'ݏ +109 h '4 .t\CSV.dial CSR C'u  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'R'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*E  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F* '& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*% n +108 h n F*~% h %& ."MFBPCR"\AS.seek 0.092ml F*X'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'@' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C's'4 +2647 h '4 C'e& h &W #tGaraging FlushPuck in Collection Clamp M&?& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'b( +416 h ( M&;& h &ӷ M&)% h % .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&[& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &P'η +91 h 'η .tHand.open M&=1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'C& h & M&% h %\  A('% h %X A(`. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +201 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (6& h &,% M&S& h &- M&7% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &B(k +166 h (k M&!& h &k A(f& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &j'Ю +89 h 'Ю A(p& h &,% ."WCR"\SE.seek 8.71 Pucks below Top Plate A(M' +175 h ' A(H& h &,% M&x& h &k ."WCR"\Elbow.seek 4 M&\\(k +7 h (k M&I& h &k A(o& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&D' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &#/ +79 h '&0 ."MFBPCR"delay 3 seconds +71 h / ."downElevator"\SE.seek :down A(-'X M&v& h &k M&0% h % ."WCR"\Forearm.seek :retract M&\ '\ +91 h '\  ."WCR"\Elbow.seek CC M& (ӷ +84 h 'X ."downElevator"\SE.coast A(5 +54 ."MFBPCR"\ATV.dial :waste2 F*g % +5 h (ӷ M&*& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +38 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +53 h ' ."WCR"\CC.seek :closed C'W(W +415 h (W ."WCR"Hand.open M&l1 +92 h 'R ."MFBPCR"\ATV.dial :air2 F*d  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *V' +36 h 1 M&L% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)G+ +7 h + S)O% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'U  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +64 h  F*:% h % ."MFBPCR"\AS.seek 0.52ml F*4' +641 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C' % h %4 S)+% h % S)% h % ."WCR"Intake.select :close S)0 +6 )$% h % ."WCR"\SSV.dial :exhaust S)"  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X  +65 h  ."MFBPCR"\ARV.dial :carrier F*:  +10 h  F*n% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*.  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*/  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*1 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +107 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.6ml F*t'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*~  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*@'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254640 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'A  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'e @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'k  +94 h  C';% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  ')  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'm'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'=  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'Y  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'`' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'C @ +75 h @ +50 S)'% h % ."WCR"\CBV.dial between CSV and waste2b C'7  +94 h  C'P% h % ."WCR"\CSV.dial between air and CTV C'S ` +57 h ` ."WCR"\CS.seek 7.288ml C'f' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'p  ."WCR"\CSV.dial CTV C'!  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'H'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'x  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'o  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'I% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'#' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'B @ +76 h @ +50 S)j% h % ."WCR"\CBV.dial between CSV and waste2b C'0  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C'g ` +56 h ` ."WCR"\CS.seek 7.288ml C'*' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'D  '\\  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '-'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'M  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'X' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'b  +151 h C'\ & h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&l% h %\  A(L% h %X A(d. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ((~ +200 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h &~ M&O& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&(& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &w'н +89 h 'н A(G& h &~ ."WCR"\SE.seek 10.71 Pucks below Top Plate A(^' +155 h ' M&J& h & A(h& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M&2, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 11.04 Pucks below Top Plate A(r'D +27 h 'D M&9% h % A(V& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&'- +21 h '- M&&& h & A(Q& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &N'н +22 h 'н ."WCR"\SE.seek 12.04 Pucks below Top Plate A('3 +36 h '3 M&F& h & M&8% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +90 h '\  ."WCR"\Elbow.seek CC M&|(ӷ +142 h (ӷ M&K& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ ."WCR"\Forearm.seek CC M&>' +91 h ' ."WCR"Hand.close M&:/ +150 h / ."WCR"\CC.seek :open C'n( +416 h ( A(5& h &~ A( & h &~ M&& h &ӷ M&+% h % ."WCR"\Forearm.seek :retract M&$'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &.( +142 h ( M&T& h & A(<& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &"' +93 h ' ."WCR"\Elbow.hold M&/, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &z1 +150 h 1 M&4% h % A(& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&'- +21 h '- M&c& h & A(1& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н A({% h %3 ."downElevator"\SE.seek :down A(_'X +100 (u% h % M&Z% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C',& h & .tHand.open M&1 +2 h 1 M&& h & M&E% h %\  .t\Elbow.seek FlushPuck::Garage M&t(- +166 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M& / +150 h / M&%& h &- M&R% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &A(ӷ +264 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'k& h &W .tHand.open M&;1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C')& h &W C'[% h % .t\CSV.dial CSR C'  +38 h  C'=% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'm'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'Y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'`& h &W C'C% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'P @ +37 h @ C'S% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CTV C'!  .t\CTV.dial CSV C'p  +38 h  +113  .t\CBV.dial :waste1 C'f @ +75 h @ .t\CS.seek 0ml C'x'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'H& h &W C'o% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'I'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'#% h %p .t\CS.seek 7ml C'B'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +38 h  .t\CBV.dial :waste2b C'0  +75 h  .t\CS.seek 0ml C']'4 +766 h '4 #t\Flushing upper manifold C'g& h &W C'v% h %4 .t\CSV.dial :flush C'\\  +76 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'M% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CBV C'b .t\CBV.dial CSV C'\  +37 h +75 .t\CTV.dial :air C'l  +151 h .t\CS.seek 0ml C'L'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'd'0 +507 h '0 .t\CSV.dial :waste C'U +37 h .t\CS.seek 0ml C'O'4 +507 h '4 C'& h &W C'(% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'G'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'J% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +506 h '0 .t\CSV.dial :waste C'9 +76 h .t\CS.seek 0ml C'V'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'&& h &W C'Q% h %4 .t\CSV.dial CSR C'F  +151 h  C'8% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'w'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'^ .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'K' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'3  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'r  +113 h  C'N% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'2'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ':' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'q  'W  +38 h  +113  .t\CBV.dial :waste1 C'5 @ +38 h @ .t\CS.seek 0ml C' '4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'+% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'T'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'>  +38 h  .t\CBV.dial :waste2b C'|  +75 h  .t\CS.seek 0ml C'z'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'4  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'c'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ '$  +38 h +75 .t\CTV.dial :air C'.  +150 h .t\CS.seek 0ml C'u'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +507 h '0 .t\CSV.dial :waste C', +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'& h &W C'E% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C' 'i +415 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'_'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'/ +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'%& h &W C'R% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +38 h @ C's% h %0 .t\CS.seek 5ml C't'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'?'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C';'4 +507 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C')% h %4 .t\CSV.dial :flush C'[  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +37 h @ C'% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV C'Y  .t\CTV.dial CSV C'm  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'`& h &W C'C% h %4 .t\CSV.dial :flush C''  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'P @ +38 h @ C'S% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CTV C'!  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'p  +75 h  .t\CS.seek 0ml C'f'4 +766 h '4 #tDisinfecting external Intake with kill C'6& h &W C'H% h %4 .t\CSV.dial CSR C'o  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'x'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'# .t\CBV.dial CSV C'I  +37 h +76 .t\CTV.dial :intake C'j  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'0' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'B  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'g'4 +606 h '4 .t\CSV.dial CSR C'v  +113 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'*' +1372 h ' .t\CSV.dial CBV C'M +113 h .t\CS.seek 0ml C'D'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&\ & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +6 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&U/ +150 h / .t\CC.seek :open C'd( +416 h ( M&& h &ӷ M&(% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&h1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'y& h & .tHand.open M&&1 +2 h 1 M&Q& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &F(- +7 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.seek :garage M&w'η +91 h 'η .tHand.close M&^/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +264 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'2(W +416 h (W C'q& h &W .tHand.open M&W1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'+% h %4 .t\CSV.dial CSR C'  +113 h  C'T% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'>'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '|% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '<'[ +462 h '[ .t\CSV.dial :waste C'4 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'z& h &W C'c% h %4 .t\CSV.dial :flush C'$  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'. +75 h .t\CTV.dial :intake C'1  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'u'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C',  +75 h #t\Flushing upper manifold C'Z& h &W C'% h %4 .t\CSV.dial :flush C'E  +76 h  .t\CS.seek 5ml C'"'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'}'i +415 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'_ +38 h .t\CS.seek 0ml C'/'4 +506 h '4 C'& h &W C'%% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'e% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'? +75 h .t\CS.seek 0ml C'k'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&n& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&Y% h % .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&'1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [mbaPhase] begun 06:04:37 #\Preparing to sleep until Sun Oct 21 06:04:37 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:04CEST21-Oct-18 #\Continuing at 06:04CEST21-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:00:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:04CEST21-Oct-18" +235186 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1909h25ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*!  +21 h ."MFBPCR"\ATV.dial :air3 F*p % +87 h % F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*o% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*I'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*B  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +65 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*g' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1911h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ARV.dial :carrier F*r  +10 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*d n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*5  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*T  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*D' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*|  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*4' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*<  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*c'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*.'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*1 n +21 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F*,'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*E' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*u  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1911h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*)  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +64 h  F*=% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +21 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*!'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*H  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1913h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*g% h %V ."MFBPCR"\AS.seek 0.5ml F*]'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F** n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +21 h ."MFBPCR"delay 2 seconds +200 F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*h  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Q n +22 h n ."MFBPCR"delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.6ml F*V'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*G  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*O  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1913h25ml.pcr F*F% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*3  +21 h ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*W% h %Z ."MFBPCR"\AS.seek 0.81ml F*T'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*|'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +65 h  F*$% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*_  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1916h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +10 h  F*K% h %V ."MFBPCR"\AS.seek 0.5ml F*:'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*m n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*C% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*S I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.53ml F*a' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*p % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +22 h n ."MFBPCR"delay 2 seconds +200 F*f% h %\  ."MFBPCR"\AS.seek 0.6ml F*x'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.65ml F*j' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*I  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*0% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1916h25ml.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F**  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +87 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*(% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*b% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +31 h  ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*N'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct1918h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ARV.dial :carrier F*<  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*c'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*,  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*u  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*E n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*}' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*"'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +22 h  ."MFBPCR"delay 7 seconds +700 F*%% h %< ."MFBPCR"\AS.seek 0.594ml F*/'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*~  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*)% h % \\ ."MFBPCR"\AS.seek 0.326ml F*='{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*K % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct1918h25ml.pcr F*[% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *`'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*'% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*X'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*@% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*p  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +64 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*#  +32 h  ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*M% h %m ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1920h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*L  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*y'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*w I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*9 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*N' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*3  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.536ml F*r'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +22 h  ."MFBPCR"delay 7 seconds +700 F*5% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5848 h '\  ."MFBPCR"delay 5 seconds +499 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*>' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1920h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*4 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*c  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*1  +151 h F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *,' +423 h ' ."MFBPCR"\ATV.dial :ASV F*u n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*Z% h % ."MFBPCR"\AS.seek 0.62ml F*E'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*}% h % ."MFBPCR"\AS.seek 0.53ml F*_' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*"% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h % H ."MFBPCR"\AS.seek 0.735ml F*s' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*t' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*~ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*)% h % zG ."MFBPCR"\AS.seek 0.365ml F*?' +7161 h ' ."MFBPCR"\ATV.dial :waste F*n n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*[ % +43 h % F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*Y% h % ."MFBPCR"\AS.seek 0.579ml F*:' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*'% h %  ."MFBPCR"\AS.seek 0.604ml F*`'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*C% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*;' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +86 h n ."MFBPCR"\ASV.dial :wasteR F*@  +22 h  ."MFBPCR"\AS.seek 0ml F*X'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *7'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*a % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*!% h % ."MFBPCR"\AS.seek 0.58ml F*6' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +21 h % ."MFBPCR"delay 3 seconds +300 F*o% h % q ."MFBPCR"\AS.seek 0.605ml F*#'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +21 h F*I% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*0 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*j  +21 h  ."MFBPCR"\AS.seek 0ml F*B'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\\  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b **' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*M n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*] n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +202 h 'R ."MFBPCR"\SPE.coast F*A  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*U n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *-' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*h  +42 h  F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *L': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*J% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *y'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Q n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *8' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*^ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *V'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*G  +64 h  F*9% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *F'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*O n +65 h n F*3% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *q' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*N I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*5  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *r': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*W n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*+ n +150 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*T  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*>% h % ."MFBPCR"\AS.seek 0.96ml F*<'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %0 ."MFBPCR"\AS.seek 0.985ml F*$' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*.% h % ."MFBPCR"\AS.seek 0.97ml F*{'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*u I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Z'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*} I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.seek 0ml F*"'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*_% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*/  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*~% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*=% h %n ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Y  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +32 h  ."MFBPCR"\ATV.dial :air3 F*; % +86 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *p' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*o% h %m ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*x  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V @CEST1539986420.12 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b **'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*\ % +108 h % ."MFBPCR"\AS.seek 0ml F*U'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540094677.99 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' ' h  P% %l h  F* *  h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *-  h  .t\SPE.configure SPEconfig F*\ 2 *(% h %V .t\AS.configure ASconfig S)h% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'b& S)#.0X'\ +91 h '\  M&|% h %\  P%& h &2 C'c& ."openPC"\PC.reconfigure PCconfig h &3 ."openPC"\PC.home.seek :open .t\CC.reconfigure CCconfig P%$x2 ^22b %<( .t\CC.home.seek :open C'$x2 ^22b '1( +222 h ( +3 ( +159 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 06:04:53 began in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:01:25CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #20) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'.& h & .tHand.open M&$1 +150 h 1 M&u& h &_ M&{% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&E/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'& h &W .tHand.open M&"1 +150 h 1 M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'e& h &W C'R% h %4 .t\CSV.dial CSR C's  +151 h  C'~% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 't% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C') +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '['4 +2010 h '4 #t\Purging external Intake C'=& h &W C'?% h %4 .t\CSV.dial :flush C'2  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'K  h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C':  +75 h #t\Flushing upper manifold C'm& h &W C'C% h %4 .t\CSV.dial :flush C'`  +76 h  .t\CS.seek 5ml C'@'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'X% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'!'4 +506 h '4 C'7& h &W C'f% h %4 .t\CSV.dial :flush C'o  +38 h  .t\CS.seek 3ml C'H'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'x @ +37 h @ C'6% h %0 .t\CS.seek 5ml C'I'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'#'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&B& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &g(ӷ +6 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&*/ +150 h / .t\CC.seek :open C'A( +416 h ( M&]& h &ӷ M&\ % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +90 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 18:01:30CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:01:25CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*k% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'4& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(l. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ( (,% +7 F*-% h %&K ."readyMFB"\ATV.dial :waste2 F*( % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *h' +350 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (b& h &,% M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &J(k +166 h (k M&L& h &k A(Q& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +89 h 'Ю A(y& h &,% .t\SE.seek 7.71 Pucks below Top Plate A(^' +185 h ' A(8& h &,% M&V& h &k .t\Elbow.seek 4 M&&(k +7 h (k M&w& h &k A(9& h &,% .t\Forearm.seek 4 + 300 counts M&G' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &O/ +150 h / ."downElevator"\SE.seek :down A('X M&3& h &k M&F% h % .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.seek CC M&+(ӷ +93 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &|' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&c1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S) % h % C'.& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*}  h  ."readyMFB"\ASV.dial :waste1 F*  h +18671 ."connect"\SSV.dial CBV .t\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X9 h 9d +100 C'N9 h 9 d +100 C'}9 h 9$d +100 C'9 h 9(d +100 C'9 h 9 ,d +100 C'E9 h 9$0d +100 C'19 h 9<4d +100 C'9 h 9S8d +100 C'"9 h 9kd +100 C'C9 h 9Td +100 C' 9 h 9kd +100 C'`9 h 9d +100 C''9 h 9d +100 C'X9 h 9d +100 C'@9 h 9d +100 C'a9 h 9d +100 C'S9 h 9d +100 C'9 h 9 d +100 C'[9 h 9 d +100 C'm9 h 96d +100 C'p9 h 9Ld +100 C'79 h 9bd +100 C'f9 h 9xd +100 C';9 h 9d +100 C'!9 h 9d +100 C'x9 h 9d +100 C'69 h 9d +100 C'o9 h 9d +100 C'H9 h 9d +100 C'#9 h 9d +100 C'j9 h 9(d +100 C'B9 h 9>d +100 C'I9 h 9Td +100 C'v9 h 9id +100 C'9 h 9d +100 C'\\9 h 9d +100 C'09 h 9d +100 C'*9 h 9d +100 C' 9 h 9d +100 C'_9 h 9d +100 C'\ 9 h 9  d +100 C'i9 h 9 d +100 C' 9 h 9 *d +100 C'M9 h 9 ?d +100 C'P9 h 9 Td +100 C'9 h 9 j d +100 C']9 h 9 $d +100 C'g9 h 9 (d +100 C'9 h 9 ,d +100 C'k9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'l9 h 9   +19 h C'N% h %4 .t\CS.seek 5ml C'}'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C'% h %p .t\CS.seek 7ml C'E'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'1 @ +38 h @ .t\CS.seek 10ml C'' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'" ` +132 h ` #tLysing Complete C'/& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&e% h %\  A(% h %X A(~. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (%(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (d& h &~ M&t& h &ӷ M&R% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &s( +142 h ( M&)& h & A(n& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &='н +90 h 'н A(?& h &~ .t\SE.seek 11.71 Pucks below Top Plate A(2'Ɛ +145 h 'Ɛ M&& h & A(K& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&:, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(Y- h -+Ɛ .t\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M&C% h % A( & h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&`'- +20 h '- M&'& h & A(X& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &@'н +22 h 'н .t\SE.seek 13.04 Pucks below Top Plate A(a'" +37 h '" M&S& h & M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +89 h '\  .t\Elbow.seek CC M&m(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&;/ +150 h / .t\CC.seek :open C'!( +415 h ( A(x& h &~ A(6& h &~ M&o& h &ӷ M&H% h % .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &j( +143 h ( M&B& h & A(I& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &v' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\\1 +150 h 1 M&0% h % A(*& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 & '- +20 h '- M&_& h & A(\ & h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i'н +22 h 'н A( % h %" ."downElevator"\SE.seek :down A(M'X +100 (P% h % M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +33 h 'X ."downElevator"\SE.coast A(5 +57 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'g @ +19 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'k ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&l1 +2 h 1 M&-& h & M&(% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&4/ +150 h / M&L& h &- M&Q% h %η .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +91 h ' .t\CC.seek :closed C'U(W +416 h (W C'8& h &W .tHand.open M&O1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%9 +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%F' +1597 h ' .t\PSV.dial PSR P%z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%q' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%G  C'^  h +75 .t\PSV.dial :mixloop P%& +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%5  h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.seek 2.5ml P%A' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2609 h ' .t\PSV.dial :mixloop P%u +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +6 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&& h &ӷ M&>% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &}'η +91 h 'η .tHand.open M&11 +150 h 1 M&{% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +90 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'"& h & M&/% h %\  A(e% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (~(,% +201 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (d& h &,% M&t& h &- M&R% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &)(k +166 h (k M&n& h &k A(?& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &+'Ю +89 h 'Ю A(& h &,% .t\SE.seek 6.71 Pucks below Top Plate A(K' +196 h ' A(Y& h &,% M&C& h &k .t\Elbow.seek 4 M& (k +6 h (k M&r& h &k A('& h &,% .t\Forearm.seek 4 + 300 counts M&X' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ +150 h / ."downElevator"\SE.seek :down M&& A(='X h &k M&s% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&@1 +150 h 1 M&:% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'[  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'x'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'6' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'o'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%H  +37 h  .t\PS.seek 2.5ml P%B' +2610 h ' .t\PSV.dial :mixloop P%I +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%f  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%m +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%\\  +151 h  .t\PS.seek :empty P%0' +2609 h ' .t\CBV.dial :waste1 C'* @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'\ @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'# ` +19 h ` .t\CSV.dial :air C'j @ +37 h @ .t\CS.seek 10ml C'P' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'g'4 +2647 h '4 .t\CBV.dial :waste1 C'k @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&l% h %\  A(-% h %X A((. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (v(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &~ M&a& h &ӷ M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &T( +143 h ( M&b& h & A(M& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &%'н +89 h 'н A(& h &~ .t\SE.seek 12.71 Pucks below Top Plate A(' +136 h ' M&4& h & A(L& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &Q' +25 h ' .t\Elbow.hold M&], +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ .t\SE.seek 13.04 Pucks below Top Plate A('" +27 h '" M&V% h % A(& h &~ .t\Forearm.force -20 .\Forearm.seek 3 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &y'- +21 h '- M&!& h & A(8& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &O'н +22 h 'н .t\SE.seek 14.04 Pucks below Top Plate A(' +37 h ' M&9& h & M&3% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.seek CC M&F' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'^( +416 h ( A(&& h &~ A(5& h &~ M&q& h &ӷ M&c% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&A& h & A(& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &.' +92 h ' .t\Elbow.hold M&u, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&W% h % A(,& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &D'- +21 h '- M&$& h & A(& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н A(U% h % ."downElevator"\SE.seek :down A('X +100 (>% h %v M&h% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +22 h 'X ."downElevator"\SE.coast A(5 +68 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +112 h  P%|% h % .t\PS.seek 0.499ml P%1' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%{ +38 h .t\PRV.dial :air P%N  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%"  +38 h .t\CBV.dial AIV C'/  +38 h .t\PRV.dial between CBV and air P%e  +19 h  .t\PRV.dial :air P%  +18 h  P%d% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %t' +251 h ' .t\PRV.dial :waste2 P%R @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %}' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&?1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&Y(- +166 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &- .t\Forearm.seek :garage M&''η +91 h 'η .tHand.close M&E/ +150 h / M&S& h &- M&% h %η .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +264 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' .t\CC.seek :closed C'X(W +416 h (W C'K& h &W .tHand.open M& 1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  C'Z& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%@ @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%:  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%[  +76 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%`' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%~' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%x' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%6 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%H  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %I'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%f  +76 h .tdelay 2 seconds +200 P%m% h %r .t\PS.seek 0.475ml P%\\' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%B  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%*'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%_  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%\  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%# +75 h .t\PTV.dial :PRV,avoiding: :puck P%j @ +75 h @ .t\PS.seek 0ml P%o' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%P' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%k +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%l' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%- +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%(  +75 h P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %a' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'i & +50 h & .t\AWV.select :waste C' +7 P%b% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %M'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%4  +37 h  .t\PS.seek 2.5ml P%L' +2610 h ' .t\PSV.dial :PML P% +37 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C';& F*! @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*8  P%9 +21 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*T I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Q'6 +66 .t\PSV.dial :waste1 P%J  +38 h  .t\PS.seek 0ml P%z' +1597 h ' .t\PSV.dial PSR P%y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%O +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%]' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*G  +22 h ' .t\PSV.dial PSR P%w +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*&'ͯ +92 h P%5% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%q' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*c % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%F' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *W' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%, +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%$' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*U% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%>' +2610 h ' .t\PSV.dial PSR P%h +113 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*|9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*v'% +336 h ' .t\PSV.dial PSR P%0 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%{' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%N +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%"' +2609 h ' .t\PSV.dial PSR P%/ +113 h P%e% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%d  +38 h  .t\PS.seek 0ml P%1' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%R +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%t' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %C' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*r I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *u'V +849 h ' .t\PSV.dial :air P%E  +113 h  .t\PS.seek 2.5ml P%S' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%'  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %+' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*p  +43 h  F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *s'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*^ I +43 h I F*K% h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +171 h ' .t\PSV.dial :mixloop P%= +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %Z' +619 h ' ."MFBPCR"\ATV.dial :waste F*@ n +128 h n ."MFBPCR"\AS.seek :empty F*7'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*:  +86 h  F*[% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*}  +43 h F*`% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'~  +37 h  C'6% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'H'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'x  'f  +38 h  +113  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C'I'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*B  +22 h ."MFBPCR"delay 3 seconds +300 F*\\% h %_ ."MFBPCR"\AS.seek 0.434ml F*_'ݏ +110 h '4 .t\CSV.dial CSR C'\  +37 h  C'*% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'#'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*j  +22 h F* % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*o n +107 h n F*k% h %& ."MFBPCR"\AS.seek 0.092ml F*P'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'-'4 +2647 h '4 C'(& h &W #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +7 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.seek CC M&a' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'M( +416 h ( M&4& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &L(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&91 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'T& h & M&% h %\  A(J% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (%(,% +200 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (y& h &,% M&z& h &- M&O% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +166 h (k M&G& h &k A(& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &w'Ю +90 h 'Ю A(5& h &,% ."WCR"\SE.seek 5.71 Pucks below Top Plate A(Q' +205 h ' A(]& h &,% M&c& h &k ."WCR"\Elbow.seek 4 M&&(k +6 h (k M&& h &k A(& h &,% ."WCR"\Forearm.seek 4 + 300 counts M& ' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &q/ +50 h '&0 ."MFBPCR"delay 3 seconds +100 h / ."downElevator"\SE.seek :down A(A'X M&,& h &k M&% h % ."WCR"\Forearm.seek :retract M&U'\ +91 h '\  ."WCR"\Elbow.seek CC M&F(ӷ +109 ."MFBPCR"\ATV.dial :waste2 F*W % +5 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +9 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +82 h ' ."WCR"\CC.seek :closed C'>(W +416 h (W ."WCR"Hand.open M&1 +61 h 'R ."MFBPCR"\ATV.dial :air2 F*D  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *|' +67 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)0% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'N  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +37 h  +113  .t\CBV.dial :waste1 C'S @ +38 h @ .t\CS.seek 0ml C'+'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'@% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '`' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  +37 h  .t\CBV.dial :waste2b C'u  +76 h  .t\CS.seek 0ml C'6'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C's  +38 h  C'H% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '~'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +709 h ' ."connect"\CSV.dial CBV C'g .t\CBV.dial CSV C':  +38 h +75 .t\CTV.dial :air C'^  +151 h .t\CS.seek 0ml C'\ '4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +506 h '0 .t\CSV.dial :waste C'I +38 h .t\CS.seek 0ml C'*'4 +506 h '4 #t\Flushing upper manifold C' & h &W C'j% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.seek 5ml C'x'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'_% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +507 h '4 C'o& h &W C'#% h %4 .t\CSV.dial :flush C'B  +37 h  .t\CS.seek 3ml C'k'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +37 h @ C'-% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'X +75 h .t\CS.seek 0ml C'4'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'2& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'a'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C')% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'l  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'3'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'T% h %4 .t\CSV.dial :flush C'J  +75 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'z% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'5  +38 h  .t\CBV.dial :waste2b C'G  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'y& h &W C']% h %4 .t\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'c'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'L' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'V  'q +38 h +75 .t\CTV.dial :intake C'w  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +607 h '4 .t\CSV.dial CSR C',  +113 h  C'Q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'$'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C' ' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'&'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C'D( +416 h ( M&h& h &ӷ M&N% h % .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &0(- +265 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&?% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&C1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &- .t\Forearm.seek :garage M&/'η +91 h 'η .tHand.close M&d/ +150 h / M&v& h &- M&%% h %η .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' .t\CC.seek :closed C'1(W +416 h (W C'& h &W .tHand.open M&>1 +150 h 1 M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'@% h %4 .t\CSV.dial CSR C'+  +113 h  C'}% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'K'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'u% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '`'[ +463 h '[ .t\CSV.dial :waste C's +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +2010 h '4 #t\Purging external Intake C'6& h &W C'~% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'^ .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'm  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'\ '4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'I  +76 h #t\Flushing upper manifold C'f& h &W C' % h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'x +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'\\ +38 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'o% h %4 .t\CSV.dial :flush C'#  +37 h  .t\CS.seek 3ml C'B'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'(% h %0 .t\CS.seek 5ml C'-'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'k'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'X'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&t& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&4' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C')( +415 h ( M&a& h &ӷ M&% h % .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &T'η +91 h 'η .tHand.open M&J1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  #t\Completed [mbaPhase] begun 06:04:53 #\Preparing to sleep until Tue Oct 23 06:04:53 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:04CEST23-Oct-18 #\Continuing at 06:04CEST23-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:56CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:04CEST23-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2109h25ml.pcr F*z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*5  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*V'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*w% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*,% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Q'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +32 h  ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +21 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*N'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*?'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*e  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *R'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2111h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*E% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*}  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*u  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*s' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*`  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*~'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*:  +108 h  ."MFBPCR"delay 7 seconds +700 F*H% h %< ."MFBPCR"\AS.seek 0.594ml F*^'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*m n +22 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*j' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F**  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*['{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2111h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*('> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +199 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*J% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*T% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +21 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ARV.dial :carrier F*Q  +10 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*.  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*i  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*h I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*F n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*?  +108 h  ."MFBPCR"delay 7 seconds +700 F*R% h %< ."MFBPCR"\AS.seek 0.594ml F*C'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*r'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.65ml F*v' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*0  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*/% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2113h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*u'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*6  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*s  +64 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*I  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +21 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\\  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2116h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*Y% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*a n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*2  +21 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*l n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*T% h %Fj ."MFBPCR"\AS.seek 0.524ml F*3' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*z I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*!' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*G % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.536ml F*5'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +107 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*c'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.65ml F*w' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*V  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*L'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2116h25ml.pcr F*,% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *.'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*A'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +64 h  F*M% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*d  +32 h  ."MFBPCR"\ATV.dial :air3 F*r % +86 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct2118h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*`  +11 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*g n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *^'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*I  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*j n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*{  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*_' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\\  +22 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Y % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct2118h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*a  +22 h ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*J% h %Z ."MFBPCR"\AS.seek 0.81ml F*4'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*P'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*O  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *]'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +31 h  ."MFBPCR"\ATV.dial :air3 F*c % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h % ."MFBPCR"\AS.seek 0.81ml F*Q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h %> ."MFBPCR"\AS.seek 0.835ml F*L'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*U'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2120h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*R'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*d  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*v% h %Fj ."MFBPCR"\AS.seek 0.524ml F*0' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*| % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*'  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +22 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*@'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*> n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*+  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*6% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*H % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2120h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*s @@T ."MFBPCR"\ASV.dial :SPEdelivery F*~  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*m  +150 h F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *I' +424 h ' ."MFBPCR"\ATV.dial :ASV F*\ n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F**  +43 h  F*f% h % ."MFBPCR"\AS.seek 0.62ml F*j'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*x n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *{'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*[% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*-' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*B% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*k n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F* ' +7162 h ' ."MFBPCR"\ATV.dial :waste F*t n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*9% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*T% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*4' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*z n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*P'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *O'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*! % +107 h % F*3% h %V ."MFBPCR"\AS.seek 0.01ml F*]' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*5% h % ."MFBPCR"\AS.seek 0.58ml F*y' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*q'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*V% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*w  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*Q  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *L'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*$ n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +202 h 'R ."MFBPCR"\SPE.coast F*i  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*. n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*h  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *A' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*F I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*?  +65 h  F*"% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *R'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*e% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *p' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*d I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*C  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *v': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*|% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*0 n +65 h n F*'% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*E I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*S  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*> n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b * 'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*@ n +151 h n ."MFBPCR"\AS.seek 0ml F*+'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*}  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*n % +43 h % F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*K% h % ."MFBPCR"\AS.seek 0.96ml F*`'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %0 ."MFBPCR"\AS.seek 0.985ml F*:' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*^% h % ."MFBPCR"\AS.seek 0.97ml F*g'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*\ I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *f'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *x'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*{  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*_ I +42 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*o n +129 h n ."MFBPCR"\AS.seek 0ml F*['V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*\\% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*9% h %n ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*1  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*4 % +87 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +21 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*]'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' @CEST1540159206.28 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *L'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*U % +107 h % ."MFBPCR"\AS.seek 0ml F*.'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540267493.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )h h  C' ' h  P% % h  F* *F h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *A  h  .t\SPE.configure SPEconfig F*\ 2 *N% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X' +27 h *k .t\Elbow.reconfigure LooseElbow .\Elbow.home.seek :clear M&$ &.,(#b &(d +47 h (d +59 / A(@& h &,? .t\SE.reconfigure SEconfig .\SE.home.seek :down A(#K-0-\ b ( 'X +149 h 'X .t\SC.reconfigure SCsloppy .\SC.home.seek :initial A($-.(\ 1@~b (}( +345 h ' +13 (  .t\Forearm.seek :retract M&n'\ +7 h '\  .t\Elbow.seek :hammer M&&(_ h '4 +92 (_ .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &+' +91 h ' M&6% h % .t\Forearm.seek :retract M&K'\ +91 h '\  M&u% h %\  P%H& C'~& h &2 &3 ."openPC"\PC.reconfigure PCconfig .t\CC.reconfigure CCconfig ."openPC"\PC.home.seek :open P%$x2 ^22b .t\CC.home.seek :open P%`( C'$x2 ^22b 'm( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 06:05:08 began in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:03:55CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #21) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'^& h & .tHand.open M&:1 +150 h 1 M&\ & h &_ M&g% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &*(- +166 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &x'η +91 h 'η .tHand.close M&j/ +150 h / M&{& h &- M&% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'& h &W .tHand.open M&[1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'#% h %4 .t\CSV.dial CSR C'-  +150 h  C'k% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'B'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'9& h &W C' % h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a  'Y h +75 .t\CTV.dial :intake C'2  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'J'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'=  +75 h #t\Flushing upper manifold C'l& h &W C'T% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'P% h %p .t\CS.seek 7ml C'z'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :air .\CS.seek 0ml C'3'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +506 h '0 .t\CSV.dial :waste C'; +38 h .t\CS.seek 0ml C'5'4 +507 h '4 C'O& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C']'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +38 h @ C'y% h %0 .t\CS.seek 5ml C'V'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'q'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'w +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'i( +416 h ( M&$& h &ӷ M&U% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Z(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &7'η +91 h 'η .tHand.open M&X1 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 18:04:00CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:03:55CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*.% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C's& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&h% F* h %\  A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (F(,% +6 F*A% h %&K ."readyMFB"\ATV.dial :waste2 F*N % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +350 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (M& h &,% M&b& h &- M&?% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &"(k +166 h (k M&& h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &e'Ю +90 h 'Ю A(R& h &,% .t\SE.seek 4.71 Pucks below Top Plate A(d' +214 h ' A(p& h &,% M&C& h &k .t\Elbow.seek 4 M&r(k +7 h (k M&/& h &k A(%& h &,% .t\Forearm.seek 4 + 300 counts M&|' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &0/ +150 h / ."downElevator"\SE.seek :down M&& h &k A(''X M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&@(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +19 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +91 h ' .t\CC.seek :closed C'~(W +416 h (W .tHand.open M&H1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)n+ +7 h + S)+% h % C'^& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'n  +38 h  .t\CS.seek 1.275ml C'g' +919 h ' #tHeating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'\   'f9 h 9d +100 C'I9 h 9d +100 C'&9 h 91d +100 C'9 h 9J d +100 C':9 h 9bd +100 C'{9 h 9zd +100 C'9 h 9d +100 C'K9 h 9d +100 C'E9 h 9 d +100 C'_9 h 9$d +100 C'9 h 9(d +100 C'*9 h 9 ,d +100 C'j9 h 9$0d +100 C'm9 h 9<4d +100 C'9 h 9S8d +100 C'[9 h 9kd +100 C'T9 h 9Td +100 C'29 h 9kd +100 C'9 h 9d +100 C'J9 h 9d +100 C'P9 h 9d +100 C'9 h 9d +100 C'!9 h 9d +100 C'z9 h 9d +100 C'39 h 9 d +100 C'9 h 9 d +100 C'l9 h 96d +100 C'G9 h 9Ld +100 C'O9 h 9bd +100 C'9 h 9xd +100 C'49 h 9d +100 C'59 h 9d +100 C'c9 h 9d +100 C'y9 h 9d +100 C'9 h 9d +100 C']9 h 9d +100 C'q9 h 9d +100 C'w9 h 9(d +100 C'9 h 9>d +100 C'V9 h 9Td +100 C',9 h 9id +100 C';9 h 9d +100 C'Q9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'\\9 h 9d +100 C'U9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C' 9 h 9 ?d +100 C'89 h 9 Td +100 C'X9 h 9 j d +100 C'$9 h 9 $d +100 C'W9 h 9 (d +100 C'L9 h 9 ,d +100 C'.9 h 9 0d +100 C'h9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9  @ +37 h @ .t\CS.seek 5ml C'f'p +1373 h 'p .t\CSV.dial CTV C'I  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'& @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C': @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'{'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'K  +19 h C'E% h %4 .t\CS.seek 5ml C'_'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C'*% h %p .t\CS.seek 7ml C'j'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'm @ +37 h @ .t\CS.seek 10ml C'' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'[ ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&(% h %\  A( % h %X A(k. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (o(~ +200 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &~ M&& h &ӷ M&#% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &-( +142 h ( M&& h & A(t& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &9'н +90 h 'н A( & h &~ .t\SE.seek 14.71 Pucks below Top Plate A(1'] +116 h '] M&B& h & A(a& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &Y' +25 h ' .t\Elbow.hold M&=, +7 h , .t\Elbow.reconfigure TightElbow A(- h -+] M&$&.,(\ #b .t\SE.seek 15.04 Pucks below Top Plate A(' +26 h ' M&T% h % A(2& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'- +21 h '- M&J& h & A(P& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н .t\SE.seek 16.04 Pucks below Top Plate A(!' +36 h ' M&z& h & M&3% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&l(ӷ +143 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'5( +416 h ( A(c& h &~ A(y& h &~ M&& h &ӷ M&]% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &w( +142 h ( M&& h & A(V& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &,' +93 h ' .t\Elbow.hold M&;, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Q1 +150 h 1 M&% h % A( & h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'- +21 h '- M&\\& h & A(U& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +22 h 'н A(% h % ."downElevator"\SE.seek :down A( 'X +100 (8% h %X M&X% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +3 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'W @ +19 h @ .t\CS.seek 10ml C'L' +7 h ' .t\CSV.dial between air and CTV C'. ` +18 h ` .t\AIV.dial :bypass C'h { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&A& h & M&N% h %\  .t\Elbow.seek FlushPuck::Garage M&<(- +166 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &- .t\Forearm.seek :garage M&b'η +91 h 'η .tHand.close M&s/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &C' +90 h ' .t\CC.seek :closed C'%(W +416 h (W C')& h &W .tHand.open M&01 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%/ +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%"' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%6 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%S' +2610 h ' ."connect"\PRV.dial CBV P%|  .t\CBV.dial PRV C'd  h +75 .t\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%'' +2610 h ' .t\PSV.dial :air P%H  +38 h  .t\PS.seek 2.5ml P%i' +2609 h ' .t\PSV.dial :mixloop P%+ +38 h .t\PS.seek 0ml P%^' +2610 h ' .t\PSV.dial :air P%v  +37 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P%g +38 h .t\PS.seek 0ml P%n' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&>& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +7 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&:/ +150 h / .t\CC.seek :open C'f( +416 h ( M&& h &ӷ M&K% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &{(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &_'η +91 h 'η .tHand.open M&m1 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'[& h & M&% h %\  A((% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (k(,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &,% M&& h &- M&#% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +166 h (k M&t& h &k A( & h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &@'Ю +89 h 'Ю A(B& h &,% .t\SE.seek 3.71 Pucks below Top Plate A(a' +224 h ' A(& h &,% M&T& h &k .t\Elbow.seek 4 M&2(k +7 h (k M&J& h &k A(P& h &,% .t\Forearm.seek 4 + 300 counts M&' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &z/ +150 h / ."downElevator"\SE.seek :down M&9& A(3'X h &k M&-% h % .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.seek CC M&Y(ӷ +133 h 'X ."downElevator"\SE.coast A(5 +9 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'~  +19 h  .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%]  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%V +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%Q  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'\\  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'U @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'q ` +19 h ` .t\CSV.dial :air C'w @ +38 h @ .t\CS.seek 10ml C'8' +2647 h ' .t\CSV.dial CTV C'X  +38 h  .t\CS.seek 0ml C'W'4 +2647 h '4 .t\CBV.dial :waste1 C'. @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'h  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&% h %\  A(A% h %X A(N. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (,(~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (& h &~ M&!& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &}( +142 h ( M&M& h & A( & h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &o'н +89 h 'н A(& h &~ .t\SE.seek 15.71 Pucks below Top Plate A(;'L +107 h 'L M&s& h & A(& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&$, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(b- h -+L .t\SE.seek 16.04 Pucks below Top Plate A(Z' +27 h ' M&p% h % A(<& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&R'- +21 h '- M&5& h & A()& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &0'н +22 h 'н .t\SE.seek 17.04 Pucks below Top Plate A(7' +36 h ' M&/& h & M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +90 h '\  .t\Elbow.seek CC M&e(ӷ +142 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'd( +416 h ( A(r& h &~ A(& h &~ M&S& h &ӷ M&H% h % .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &+( +143 h ( M&i& h & A(v& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &^' +92 h ' .t\Elbow.hold M&g, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &F1 +150 h 1 M&>% h % A(I& h &~ .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &&'- +21 h '- M&:& h & A(?& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & 'н +22 h 'н A(%% h % ."downElevator"\SE.seek :down A('X +93 h 'X ."downElevator"\SE.coast A(5 +7 (K% h %X M&% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%*  +113 h  P%u% h % .t\PS.seek 0.499ml P%m' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%\ +38 h .t\PRV.dial :air P%E  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%[  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%(  +18 h  .t\PRV.dial :air P%  +19 h  P%D% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%# @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %_' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C't& h & .tHand.open M& 1 +2 h 1 M&B& h & M&{% h %\  .t\Elbow.seek FlushPuck::Garage M&j(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.seek :garage M&J'η +91 h 'η .tHand.close M&P/ +150 h / M&z& h &- M&9% h %η .t\Forearm.seek :retract M&-'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'a& h &W .tHand.open M&21 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  C'f& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%=  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%~% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%k' +1094 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 0ml P%c' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%]  +37 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %V'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%Q' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%\\  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%U  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%q +75 h .t\PTV.dial :PRV,avoiding: :puck P%w @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%X  +75 h  .t\PS.seek 2.5ml P%8' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%. +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%h' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%A +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %!' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%M% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %Y' +1312 h ' .t\PSV.dial :air P%s  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%b +37 h .t\PS.seek 0ml P%p' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '<& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'5& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*) @@T .t\AIV.dial :bypass F*4  .t\PSV.dial PSR P%/ +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%o' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*} I +129 h I F*;% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%"  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%R +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%Z' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%$' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*|  +22 h ' .t\PSV.dial PSR P%C +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*r'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%S' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*H % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*F% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *>' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%I +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%:' +217 ."MFBPCR"\ATV.dial :AIV F*? I +43 h I F*%% h % ."MFBPCR"\AS.seek 0.469ml F*'' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +2610 h ' .t\PSV.dial PSR P% +113 h P%^% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%&' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%7  +38 h  .t\PS.seek 0ml P%*' +739 h ' ."MFBPCR"delay 5 seconds +500 F*u9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F* % h % ."MFBPCR"\AS.seek 0.041ml F*,'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%\ ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%E +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%[' +2609 h ' .t\PSV.dial PSR P% +113 h P%(% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%D  +37 h  .t\PS.seek 0ml P%m' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%# +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %B'k +2679 h 'k .t\PSV.dial :waste1 P%{  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*T I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *g'V +849 h ' .t\PSV.dial :air P%P  +113 h  .t\PS.seek 2.5ml P%z' +2610 h ' .t\PSV.dial :mixloop P%9 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %@' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *-'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*d I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*2' +170 h ' .t\PSV.dial :mixloop P%3 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %f' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*O'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*=  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *~' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*_  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *L'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'k  +37 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C']'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  '  +38 h  +113  .t\CBV.dial :waste1 C'l @ +38 h @ .t\CS.seek 0ml C'V'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*Q% h %_ ."MFBPCR"\AS.seek 0.434ml F*\\'ݏ +110 h '4 .t\CSV.dial CSR C'U  +37 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'q'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*w  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*X'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*.% h %& ."MFBPCR"\AS.seek 0.092ml F*8'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'A'4 +2647 h '4 C'N& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.seek CC M&!' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&s& h &ӷ M&Y% h % .t\Forearm.seek :retract M&b'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &4'η +91 h 'η .tHand.open M&/1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'}& h & M&;% h %\  A("% h %X A(x. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (o(,% +200 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (R& h &,% M&6& h &- M&0% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &Z(k +166 h (k M&|& h &k A(& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &C'Ю +89 h 'Ю A(& h &,% ."WCR"\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') A($& h &,% M&H& h &k ."WCR"\Elbow.seek 4 M&r(k +7 h (k M&v& h &k A(e& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&F' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ +19 h '&0 ."MFBPCR"delay 3 seconds +131 h / ."downElevator"\SE.seek :down M&I& A(i'X h &k M&?% h % ."WCR"\Forearm.seek :retract M&%'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +78 ."MFBPCR"\ATV.dial :waste2 F*> % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +21 h (ӷ M&:& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' + h 'X ."downElevator"\SE.coast A(5 +90 h ' ."WCR"\CC.seek :closed C'K(W +416 h (W ."WCR"Hand.open M&71 +32 h 'R ."MFBPCR"\ATV.dial :air2 F*&  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *u' +96 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)'+ +7 h + S)% h % C'*& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'E  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*: n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*r I +85 h I F*8% h % ."MFBPCR"\AS.seek 0.12ml F*%' +626 h ' ."MFBPCR"\ATV.dial :air3 F*i % +21 h % F* % h % ."MFBPCR"\AS.seek 0.13ml F*&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % ."MFBPCR"\AS.seek 0.255ml F*E'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*o% h %X ."MFBPCR"\AS.seek 0.24ml F*'' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*( n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b * '5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*\ @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*, I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*u'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*{  +43 h  #"MFBPCR"\Swish #1 of 2 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *T'\  +1032 h '\  F*t% h %\  ."MFBPCR"\AS.seek 0.5ml F*P'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*9'\  +1032 h '\  F*B% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *j' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*g % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +3472 h 'V ."MFBPCR"\SPE.coast F*J  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2309h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*a  +64 h  ."MFBPCR"\ARV.dial :carrier F*3  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*@'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*d  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*_  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*k' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*]  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*c% h % ."MFBPCR"\AS.seek 0.53ml F*W' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*l % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Q  +107 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 F*X  +65 h ."MFBPCR"\ARV.dial :air1 F*.  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*h% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254700 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'N @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)M% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C's% h % ."WCR"\CSV.dial between air and CTV C'1 ` +18 h ` ."WCR"\CS.seek 7.288ml C'!' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'<  'Y  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'/  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '5' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'b  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C';% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'x' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'R' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'} @ +75 h @ +50 S)"% h % ."WCR"\CBV.dial between CSV and waste2b C'0  +94 h  C'4% h % ."WCR"\CSV.dial between air and CTV C'6 ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'|  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'p'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'$  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'v  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'e  +38 h  C'H% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'S' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'Z @ +75 h @ +50 S)C% h % ."WCR"\CBV.dial between CSV and waste2b C'?  +94 h  C':% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'>' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'F  'r  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '8'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'i' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'%& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&7% h %\  A(% h %X A(&. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (*(~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (+& h &~ M&& h &ӷ M&^% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &o( +142 h ( M&E& h & A((& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A('& h &~ ."WCR"\SE.seek 16.71 Pucks below Top Plate A(D'; +95 h '; M&[& h & A( & h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &,' +25 h ' ."WCR"\Elbow.hold M&m, +7 h , ."WCR"\Elbow.reconfigure TightElbow A( - M&$&.,(\ #b h -+; ."WCR"\SE.seek 17.04 Pucks below Top Plate A({' +27 h ' M&#% h % A(u& h &~ ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &t'- +21 h '- M&T& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &P'н +22 h 'н ."WCR"\SE.seek 18.04 Pucks below Top Plate A(B' +37 h ' M&9& h & M&G% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&g(ӷ +142 h (ӷ M&j& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.seek CC M&a' +91 h ' ."WCR"Hand.close M&3/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(z& h &~ A(@& h &~ M&2& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&='\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &d( +143 h ( M&_& h & A(& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' ."WCR"\Elbow.hold M&~, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &y1 +150 h 1 M&-% h % A(]& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&c'- +21 h '- M&k& h & A(l& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &n'н +22 h 'н A(W% h % ."downElevator"\SE.seek :down A(Q'X +83 h 'X ."downElevator"\SE.coast A(5 +17 (U% h %X M&% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'V& h & .tHand.open M& 1 +2 h 1 M&& h & M&w% h %\  .t\Elbow.seek FlushPuck::Garage M&L(- +165 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&.'η +91 h 'η .tHand.close M&h/ +150 h / M&& h &- M&q% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'<% h % .t\CSV.dial CSR C'!  +37 h  C'/% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'b% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'; +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '5'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'x& h &W C'}% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '0'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'6% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '|  +37 h  +113  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'$'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'p& h &W C'v% h %4 .t\CSV.dial :flush C'e  +76 h  .t\CS.seek 5ml C'H'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'S% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  +37 h  .t\CBV.dial :waste2b C'?  +76 h  .t\CS.seek 0ml C':'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'I% h %4 .t\CSV.dial :flush C'F  +75 h  .t\CS.seek 5ml C'r'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '%  +37 h +76 .t\CTV.dial :air C'7  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C''4 +507 h '4 C'^& h &W C'E% h %4 .t\CSV.dial :flush C'(  +38 h  .t\CS.seek 3ml C'''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'[% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'#'0 +507 h '0 .t\CSV.dial :waste C'u +75 h .t\CS.seek 0ml C'T'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C' % h %4 .t\CSV.dial CSR C'9  +150 h  C'G% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'o' +416 h ' ."connect"\CSV.dial CBV C'D .t\CBV.dial CSV +113 h .t\CTV.dial :intake C',  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'j' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'J  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 't'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'{  +113 h  C'P% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'm'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  '  +37 h  +113  .t\CBV.dial :waste1 C'z @ +38 h @ .t\CS.seek 0ml C'@'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'2  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  +37 h  .t\CBV.dial :waste2b C'g  +76 h  .t\CS.seek 0ml C'y'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'-  +38 h  C']% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'k'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W  '= +38 h +75 .t\CTV.dial :air C'd  +151 h .t\CS.seek 0ml C'U'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'V +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C'& h &W C'w% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +38 h @ C'\\% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CBV C'Q .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'~ +38 h .t\CS.seek 0ml C'h'4 +506 h '4 C'& h &W C'q% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'.'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +37 h @ C'A% h %0 .t\CS.seek 5ml C'L'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C's'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'1& h &W C'Y% h %4 .t\CSV.dial :flush C'<  +38 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'% h %p .t\CS.seek 7ml C'i'i +416 h 'i ."connect"\CSV.dial CTV C'b  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'; @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'x& h &W C'}% h %4 .t\CSV.dial :flush C'"  +75 h  .t\CS.seek 5ml C'5'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'6% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'|  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'R& h &W C'p% h %4 .t\CSV.dial CSR C'v  +37 h  C'e% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'$'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'S .t\CBV.dial CSV C'H  +38 h +75 .t\CTV.dial :intake C'C  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'?' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Z  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'I  +113 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C':'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +1373 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'r'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&%& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&+/ +150 h / .t\CC.seek :open C'&( +416 h ( M&^& h &ӷ M&E% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &''η +90 h 'η .tHand.open M& 1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'#& h & .tHand.open M&1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &9(- +7 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&D/ +150 h / M&,& h &- M&o% h %η .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C'K& h &W .tHand.open M&1 +150 h 1 M&z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'2& h &W C'% h %4 .t\CSV.dial CSR C'@  +113 h  C'_% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'a'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'- +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ']'4 +2010 h '4 #t\Purging external Intake C'y& h &W C'k% h %4 .t\CSV.dial :flush C'W  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'd +76 h .t\CTV.dial :intake C'l  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'U'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'V  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'w  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'B% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c +113 h .t\CTV.dial :air .\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +506 h '0 .t\CSV.dial :waste C'n +38 h .t\CS.seek 0ml C'~'4 +506 h '4 C'*& h &W C'% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'N% h %0 .t\CS.seek 5ml C'A'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'( +416 h ( M&!& h &ӷ M&b% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &;(- +265 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &}'η +91 h 'η .tHand.open M&"1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Completed [mbaPhase] begun 06:05:08 #\Preparing to sleep until Thu Oct 25 06:05:08 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:05CEST25-Oct-18 #\Continuing at 06:05CEST25-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:05:30CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:05CEST25-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2309h25ml.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*v% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*H'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +64 h  F*I% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *F'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*8  +32 h  ."MFBPCR"\ATV.dial :air3 F*+ % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +21 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *#'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2311h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*{  +11 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*2  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *@'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*_  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*g  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*-' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*k'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*W  +107 h  ."MFBPCR"delay 7 seconds +700 F*]% h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*l n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*V'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*w' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*B% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2311h25ml.pcr F*Q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*N'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*L% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*/% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*x  +31 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*)'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*0% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2313h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*F  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*:'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*> n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *M'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*^ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*('< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*#% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*T'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*u % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2313h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +64 h  F*W% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *]'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F**% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*n  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *<'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2316h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*x  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*}% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*6 I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*)' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*| % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*p  +107 h  ."MFBPCR"delay 7 seconds +700 F*5% h %< ."MFBPCR"\AS.seek 0.594ml F*v'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*$'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.65ml F*C' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*H  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Z  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2316h25ml.pcr F*I% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*>  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *+'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*7% h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*M'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*o% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*K% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *-'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct2318h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*= n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*V  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 F*#,,'T0 b ."MFBPCR"\AS.seek 0.506ml F*'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*w n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*c% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*\  +22 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*X  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*n  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*~'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +21 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*A  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*.  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct2318h25ml.pcr F*<% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*!  +22 h ."MFBPCR"\ATV.dial :air3 F*m % +86 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *x'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +21 h F*"% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*$ % +87 h % F*H% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*F'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*:  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2320h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*#'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *''Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*u % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*P' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*J  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +22 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.6ml F*t'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*@  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*g  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2320h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*- @@T ."MFBPCR"\ASV.dial :SPEdelivery F*k  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*l  +151 h F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *V' +424 h ' ."MFBPCR"\ATV.dial :ASV F*U n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*w'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*c n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *\ 'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*B % +43 h % F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b **' +424 h ' ."MFBPCR"\ATV.dial :air3 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*h' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +21 h % ."MFBPCR"delay 3 seconds +300 F*~% h % H ."MFBPCR"\AS.seek 0.735ml F*A' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*L' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*. n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*< % +43 h % F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*b% h % ."MFBPCR"\AS.seek 0.579ml F*3' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %  ."MFBPCR"\AS.seek 0.604ml F*x'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*}% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*s' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*6 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*i'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *0'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*) % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*R' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h % q ."MFBPCR"\AS.seek 0.605ml F*S'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*H% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*e' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*? n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*C  +21 h  ."MFBPCR"\AS.seek 0ml F*Z'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*F  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *>' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*: n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *%'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*7'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*+ n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*^  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *8' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*[% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *#'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*(% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*D I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *,': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*'  +65 h  F*u% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *9'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *K' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*P I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*z  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *{': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *2'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*@'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*_  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*t % +43 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*a% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %0 ."MFBPCR"\AS.seek 0.985ml F*W' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*d% h % ."MFBPCR"\AS.seek 0.97ml F*='c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*U I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *c'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*\  +43 h  F*B% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *X' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Q' +9987 h ' ."MFBPCR"\ATV.dial AIV F*\\ I +43 h I ."MFBPCR"\AS.seek 0.02ml F**' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*n% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*~  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +43 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +21 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*m  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*b  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*s % +87 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' @CEST1540332021.71 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*% % +108 h % ."MFBPCR"\AS.seek 0ml F*+'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 &6 P%5 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540440308.88 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )^ h  C' ' h  P% %7 h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *8  h  .t\SPE.configure SPEconfig F*\ 2 *E% h %V .t\AS.configure ASconfig S) % F*#,,'T(^20( b h % .t\SS.configure SSconfig C' & S)#.0X 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:06:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #22) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'd& h & .tHand.open M&W1 +150 h 1 M&U& h &_ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &c'η +91 h 'η .tHand.close M&w/ +150 h / M&\ & h &- M&B% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'n(W +416 h (W C'*& h &W .tHand.open M&1 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'N& h &W C'q% h %4 .t\CSV.dial CSR C'A  +151 h  C'.% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'L% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C'Y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '<'4 +2010 h '4 #t\Purging external Intake C'/& h &W C'% h %4 .t\CSV.dial :flush C'm  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'b' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! 'j  h +75 .t\CTV.dial :intake C'1  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'"'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'3  +75 h #t\Flushing upper manifold C'& h &W C'}% h %4 .t\CSV.dial :flush C'x  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C's @ +38 h @ C'i% h %p .t\CS.seek 7ml C'6'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C') +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'|'0 +507 h '0 .t\CSV.dial :waste C'5 +38 h .t\CS.seek 0ml C''4 +506 h '4 C'0& h &W C'% h %4 .t\CSV.dial :flush C'v  +38 h  .t\CS.seek 3ml C'p'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'R% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'S'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C'C +75 h .t\CS.seek 0ml C'e'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&Z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'( +416 h ( M&:& h &ӷ M&%% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &;(- +264 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&O1 +150 h 1 M&`% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 18:06:31CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:06:27CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'-& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&^% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +7 F*8% h %&K ."readyMFB"\ATV.dial :waste2 F*E % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +350 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &,% M&M& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &[(k +166 h (k M&& h &k A( & h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &('Ю +89 h 'Ю A(#& h &,% .t\SE.seek 1.71 Pucks below Top Plate A(D': +244 h ': A(G& h &,% M&T& h &k .t\Elbow.seek 4 M&(k +7 h (k M&& h &k A(u& h &,% .t\Forearm.seek 4 + 300 counts M&o' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&'& A(J'X h &k M&9% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'](W +416 h (W .tHand.open M&k1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)t+ +7 h + S)@% h % C'd& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +387 h ' +50 .t\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'}9 h 9Td +100 C'!9 h 9kd +100 C'x9 h 9d +100 C'"9 h 9d +100 C'i9 h 9d +100 C'9 h 9d +100 C')9 h 9d +100 C'69 h 9d +100 C'9 h 9 d +100 C'<9 h 9 d +100 C'9 h 96d +100 C'|9 h 9Ld +100 C'09 h 9bd +100 C'9 h 9xd +100 C's9 h 9d +100 C'9 h 9d +100 C'$9 h 9d +100 C'R9 h 9d +100 C'v9 h 9d +100 C'p9 h 9d +100 C'S9 h 9d +100 C'C9 h 9(d +100 C'Z9 h 9>d +100 C'H9 h 9Td +100 C'I9 h 9id +100 C'59 h 9d +100 C'F9 h 9d +100 C'?9 h 9d +100 C'>9 h 9d +100 C'h9 h 9d +100 C'n9 h 9d +100 C'%9 h 9  d +100 C'f9 h 9 d +100 C'X9 h 9 *d +100 C'9 h 9 ?d +100 C'49 h 9 Td +100 C'O9 h 9 j d +100 C':9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C'^9 h 9 4d +100 C'9 h 9 8d +100 C'79 h 9 & h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&h'- +21 h '- M&n& h & A(%& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &f'н +22 h 'н A(X% h % ."downElevator"\SE.seek :down A('X +74 h 'X ."downElevator"\SE.coast A(5 +26 (4% h %X M&O% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +90 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C' @ +18 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'^ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&71 +2 h 1 M&8& h & M&E% h %\  .t\Elbow.seek FlushPuck::Garage M&`(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&M'η +91 h 'η .tHand.close M&-/ +150 h / M&& h &- M& % h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &T' +91 h ' .t\CC.seek :closed C'u(W +416 h (W C'+& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%'% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +38 h  .t\PS.seek 0ml P%9' +1597 h ' .t\PSV.dial PSR P%y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%K' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%o  C'D  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%J  h .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%k  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%@ +37 h .t\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial :air P%,  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.seek 0ml P%t' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &g(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'( +416 h ( M&B& h &ӷ M&a% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &\ (- +264 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\\'η +91 h 'η .tHand.open M&l1 +150 h 1 M&U% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'& h & M&~% h %\  A(N% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (.(,% +201 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (&& h &,% M&L& h &- M&q% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &Y(k +166 h (k M&& h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +89 h 'Ю A(& h &,% .t\SE.seek 0.71 Pucks below Top Plate A(j'K +253 h 'K A(b& h &,% M&}& h &k .t\Elbow.seek 4 M&!(k +7 h (k M&"& h &k A(i& h &,% .t\Forearm.seek 4 + 300 counts M&{' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &6/ +150 h / ."downElevator"\SE.seek :down A('X M&/& h &k M&A% h % .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.seek CC M&1(ӷ +143 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &x' +20 h 'X ."downElevator"\SE.coast A(5 +70 h ' .t\CC.seek :closed C'e(W +416 h (W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'<  +151 h  .t\CSV.dial CTV C']  +19 h  .t\CS.seek 5ml C'$'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'v'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%p  +38 h  .t\PS.seek 2.5ml P%Z' +2610 h ' .t\PSV.dial :mixloop P%H +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%F  +150 h  .t\PS.seek :empty P%?' +2610 h ' .t\CBV.dial :waste1 C'> @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'n  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'% @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'X  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'S ` +19 h ` .t\CSV.dial :air C'C @ +38 h @ .t\CS.seek 10ml C'4' +2647 h ' .t\CSV.dial CTV C'O  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'^  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&7% h %\  A(8% h %X A(E. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (I(~ +201 h (~ .t\SC.reconfigure SCloose A($.(\ 1~b (h& h &~ M&)& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &f( +142 h ( M&_& h & A( & h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +90 h 'н A(*& h &~ .t\SE.seek 18.71 Pucks below Top Plate A(5' +76 h ' M&-& h & A(& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b & ' +26 h ' .t\Elbow.hold M&:, +6 h , .t\Elbow.reconfigure TightElbow A(M- M&$&.,(\ #b h -+ .t\SE.seek 19.04 Pucks below Top Plate A(;' +27 h ' M&G% h % A(`& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&#'- +20 h '- M&& h & A(+& h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'н +23 h 'н .t\SE.seek 20.04 Pucks below Top Plate A(' +36 h ' M&& h & M&'% h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +89 h '\  .t\Elbow.seek CC M&((ӷ +143 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&9' +91 h ' .tHand.close M&o/ +150 h / .t\CC.seek :open C'D( +416 h ( A(& h &~ A(J& h &~ M&K& h &ӷ M&k% h % .t\Forearm.seek :retract M&z'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &@( +143 h ( M&& h & A(,& h &~ .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &d' +92 h ' .t\Elbow.hold M&=, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(V& h &~ .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&r'- +20 h '- M&W& h & A( & h &~ .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &2'н +22 h 'н A(u% h % ."downElevator"\SE.seek :down A(B'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (a% h %X M& % h %н .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +90 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%g% h % .t\PS.seek 0.499ml P%l' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%U +38 h .t\PRV.dial :air P%P  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'~  +38 h .t\PRV.dial between CBV and air P%N  +19 h  .t\PRV.dial :air P%  +19 h  P%&% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %L' +250 h ' .t\PRV.dial :waste2 P%q @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %\\' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&\ % h %\  .t\Elbow.seek FlushPuck::Garage M&w(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&"'η +91 h 'η .tHand.close M&i/ +150 h / M&6& h &- M&/% h %η .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C'j(W +416 h (W C'{& h &W .tHand.open M&!1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%3  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%<  +75 h  .tdelay 2 seconds +200 P%]% h % .t\PS.seek 1.499ml P%x' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%.' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%$' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%p  +38 h  .tdelay 2 seconds +200 P%t% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %H'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%F' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%Z  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%>'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%n  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %X' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%S +75 h .t\PTV.dial :PRV,avoiding: :puck P%C @ +76 h @ .t\PS.seek 0ml P%v' +2609 h ' .t\PSV.dial :air P%O  +76 h  .t\PS.seek 2.5ml P%4' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%^' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%7' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%8 +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%E  +75 h P%h% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %)' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%_% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'* { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %1' +1313 h ' .t\PSV.dial :air P%-  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%M +38 h .t\PS.seek 0ml P%G' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '`& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& F*+ @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*s  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%'% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*f I +128 h I F*5% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b * '6 +66 .t\PSV.dial :waste1 P%[  +38 h  .t\PS.seek 0ml P%y' +1597 h ' .t\PSV.dial PSR P%# +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%:' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*o  +22 h ' .t\PSV.dial PSR P%T +21 h  F*e% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%J% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%K' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*k % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%,  +38 h  .t\PS.seek 0ml P%9' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*(  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%V +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%W' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*u% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%@ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +2610 h ' .t\PSV.dial PSR P% +113 h P%d% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%r' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P% ' +740 h ' ."MFBPCR"delay 5 seconds +500 F*g9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*B n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2% h % ."MFBPCR"\AS.seek 0.041ml F*I'% +336 h ' .t\PSV.dial PSR P%? +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%U' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%~ +113 h P%N% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%l' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%L' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%\  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %b' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*} I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *='V +850 h ' .t\PSV.dial :air P%i  +113 h  .t\PS.seek 2.5ml P%6' +2609 h ' .t\PSV.dial :mixloop P%/ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %Q' +5107 h ' .t\PSV.dial :air P%"  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*|  +43 h  F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *A'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*D I +43 h I F*{% h %Q ."MFBPCR"\AS.seek 0.01ml F*!' +171 h ' .t\PSV.dial :mixloop P%m +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*0'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*3  +85 h  F*<% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *]' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*\\  +43 h F*x% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'.  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C't' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'H'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*Z  +22 h ."MFBPCR"delay 3 seconds +300 F*F% h %_ ."MFBPCR"\AS.seek 0.434ml F*n'ݏ +110 h '4 .t\CSV.dial CSR C'%  +38 h  C'>% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'S'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*C  +21 h F*X% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*O'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*v n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*4'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'^  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'8'4 +2648 h '4 C'E& h &W #tGaraging FlushPuck in Collection Clamp M&h& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&*/ +150 h / .t\CC.seek :open C' ( +416 h ( M&-& h &ӷ M&1% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &s'η +91 h 'η .tHand.open M&1 +150 h 1 M&'% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'f& h & M&5% h %\  A([% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +200 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (#& h &,% M&y& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(k +166 h (k M&o& h &k A(e& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &T'Ю +90 h 'Ю A(J& h &,% ."WCR"\SE.seek -0.29 Pucks below Top Plate A( '\\ +264 h '\\ A(:& h &,% M&k& h &k ."WCR"\Elbow.seek 4 M&(k +7 h (k M&,& h &k A((& h &,% ."WCR"\Forearm.seek 4 + 300 counts M&' +12 h '&0 ."MFBPCR"delay 3 seconds +10 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &K/ +150 h / ."downElevator"\SE.seek :down M&V& A('X h &k M& % h % ."WCR"\Forearm.seek :retract M&u'\ +91 h '\  ."WCR"\Elbow.seek CC M&9(ӷ +49 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R +50 h (ӷ M&W& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &d' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' ."WCR"\CC.seek :closed C'a(W +416 h (W ."WCR"Hand.open M&1 +3 h 'R ."MFBPCR"\ATV.dial :air2 F*r  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *g' +126 h 1 M&B% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)?% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'P  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *H% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *C' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*X% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *n' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*v% h % ."MFBPCR"\AS.seek 0.279ml F*'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*O n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*^ % +42 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *E'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*h I +21 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F*_'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F** % +21 h % F*Y% h %H ."MFBPCR"\AS.seek 0.069ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +21 h F*-% h % ."MFBPCR"\AS.seek 0.769ml F*1' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*''  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*M I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *f' +4416 h ' ."MFBPCR"\ATV.dial :waste F*5 n +129 h n ."MFBPCR"\AS.seek :empty F*['R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*c % +43 h % F*#% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*s% h % ."MFBPCR"\AS.seek 0.579ml F*o' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +21 h % ."MFBPCR"delay 3 seconds +300 F*J% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +21 h F*:% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*, n +86 h n ."MFBPCR"\ASV.dial :wasteR F*(  +43 h  ."MFBPCR"\AS.seek 0ml F*K'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*; % +107 h % F*T% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*W n +108 h n ."MFBPCR"\ASV.dial :wasteR F* % h %  ."MFBPCR"\AS.seek 0.09ml F* ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*4% h % ."MFBPCR"\AS.seek 0.12ml F*u' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F* % h % ."MFBPCR"\AS.seek 0.13ml F*r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*B% h % ."MFBPCR"\AS.seek 0.23ml F*?' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h % ."MFBPCR"\AS.seek 0.255ml F*P'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*N n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*~  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*U @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*I I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*g'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\  +43 h  #"MFBPCR"\Swish #1 of 2 F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *}'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*i'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*b% h %(A ."MFBPCR"\AS.seek 0.6ml F*/'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*Q'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*|% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *w' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*= % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *6'V +3472 h 'V ."MFBPCR"\SPE.coast F*"  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2509h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*{  +64 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*L% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *A'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*p  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*F  +107 h  ."MFBPCR"delay 7 seconds +700 F*%% h %< ."MFBPCR"\AS.seek 0.594ml F*>'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F*H% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*v' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +42 h  ."MFBPCR"\ASV.dial :waste1 F*O  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*h % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254730 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'E @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'Y @ +76 h @ +50 S)_% h % ."WCR"\CBV.dial between CSV and waste2b C'j  +94 h  C'-% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C')' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'1  '`  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '7'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'M  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C''  +37 h  C'5% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'c' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '#' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'f @ +75 h @ +50 S)[% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C's% h % ."WCR"\CSV.dial between air and CTV C'y ` +57 h ` ."WCR"\CS.seek 7.288ml C'e' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'J  'o  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'G'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C':  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C',  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'(  +38 h  C'k% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'K' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)T% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'W% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'u& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(B% h %X A(r. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( (~ +201 h (~ ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (@& h &~ M&?& h &ӷ M&d% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&P& h & A(N& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~'н +90 h 'н A(z& h &~ ."WCR"\SE.seek 19.71 Pucks below Top Plate A(&' +66 h ' M&& h & A(& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &I' +25 h ' ."WCR"\Elbow.hold M&l, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 20.04 Pucks below Top Plate A(\ ' +27 h ' M&q% h % A(g& h &~ ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'- +20 h '- M&}& h & A(b& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i'н +23 h 'н ."WCR"\SE.seek 21.04 Pucks below Top Plate A(' +36 h ' M&/& h & M&|% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +89 h '\  ."WCR"\Elbow.seek CC M&=(ӷ +143 h (ӷ M&w& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ ."WCR"\Forearm.seek CC M&{' +91 h ' ."WCR"Hand.close M&m/ +150 h / ."WCR"\CC.seek :open C'L( +416 h ( A(6& h &~ A(& h &~ M&!& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&3'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &D( +142 h ( M&\\& h & A(x& h &~ ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &<' +93 h ' ."WCR"\Elbow.hold M&], +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&A% h % A(p& h &~ ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&$'- +20 h '- M&.& h & A(& h &~ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &t'н +23 h 'н A(% h % ."downElevator"\SE.seek :down A(F'X +53 h 'X ."downElevator"\SE.coast A(5 +47 (%% h %X M&% h %н ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&>1 +2 h 1 M&X& h & M&C% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&^/ +150 h / M&v& h &- M&S% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'j& h &W .tHand.open M&-1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'`& h &W C'1% h % .t\CSV.dial CSR C')  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'M% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '''[ +463 h '[ .t\CSV.dial :waste C'5 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'c& h &W C'f% h %4 .t\CSV.dial :flush C'[  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C's @ +37 h @ C'y% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  'J  +38 h  +113  .t\CBV.dial :waste1 C'e @ +75 h @ .t\CS.seek 0ml C':'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'G& h &W C',% h %4 .t\CSV.dial :flush C'(  +75 h  .t\CS.seek 5ml C'k'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'K% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'T  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing upper manifold C' & h &W C'V% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C' % h %p .t\CS.seek 7ml C'4'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'u  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'B'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +506 h '0 .t\CSV.dial :waste C'@ +38 h .t\CS.seek 0ml C'?'4 +506 h '4 C'd& h &W C'P% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'9'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +506 h '0 .t\CSV.dial :waste C'g +75 h .t\CS.seek 0ml C'}'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'b& h &W C'/% h %4 .t\CSV.dial CSR C'|  +151 h  C'2% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'~'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& +113 h .t\CTV.dial :intake C'I  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'w' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'"  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'\  +113 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'l'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Q  'a  +38 h  +113  .t\CBV.dial :waste1 C'6 @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'!  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +38 h  .t\CBV.dial :waste2b C'=  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'A  +37 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '.'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '3  +37 h +76 .t\CTV.dial :air C'D  +150 h .t\CS.seek 0ml C'%'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'H +38 h .t\CS.seek 0ml C'>'4 +507 h '4 #t\Flushing upper manifold C'X& h &W C'C% h %4 .t\CSV.dial :flush C'<  +37 h  .t\CS.seek 5ml C'$'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'F% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV C'O .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C't'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'] +37 h .t\CS.seek 0ml C'^'4 +507 h '4 C'v& h &W C'S% h %4 .t\CSV.dial :flush C'Z  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C'8% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'h'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +506 h '0 .t\CSV.dial :waste C'j +76 h .t\CS.seek 0ml C'-'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'`% h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.seek 5ml C')'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'Y% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  '7  +37 h  +113  .t\CBV.dial :waste1 C'5 @ +38 h @ .t\CS.seek 0ml C'''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'c& h &W C'f% h %4 .t\CSV.dial :flush C'[  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C's @ +37 h @ C'y% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  +38 h  .t\CBV.dial :waste2b C'J  +75 h  .t\CS.seek 0ml C'e'4 +766 h '4 #tDisinfecting external Intake with kill C'#& h &W C'G% h %4 .t\CSV.dial CSR C',  +38 h  C'(% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C':'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'K .t\CBV.dial CSV C'k  +38 h +75 .t\CTV.dial :intake C'T  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C' ' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'V  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'W'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&u& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'r( +416 h ( M&d& h &ӷ M&P% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &?(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&91 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&b1 +2 h 1 M&/& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &|(- +6 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&&/ +150 h / M&I& h &- M&% h %η .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +265 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'l(W +415 h (W C'Q& h &W .tHand.open M&a1 +150 h 1 M&6% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'!& h &W C'% h %4 .t\CSV.dial CSR C'  +113 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'{'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '=% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'x'[ +463 h '[ .t\CSV.dial :waste C'A +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Purging external Intake C'R& h &W C'.% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'%'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'H  +75 h #t\Flushing upper manifold C'& h &W C'X% h %4 .t\CSV.dial :flush C'C  +75 h  .t\CS.seek 5ml C'<'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +37 h @ C'% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CBV C'$ .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'O'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C't +37 h .t\CS.seek 0ml C']'4 +507 h '4 C' & h &W C'v% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 3ml C'Z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +38 h @ C'E% h %0 .t\CS.seek 5ml C'8'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'h +76 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&L& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'Y( +416 h ( M&)& h &ӷ M&M% h % .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &f'η +91 h 'η .tHand.open M&[1 +150 h 1 M&'% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Completed [mbaPhase] begun 06:05:24 #\Preparing to sleep until Sat Oct 27 06:05:24 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 06:05CEST27-Oct-18 #\Continuing at 06:05CEST27-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:07:57CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 06:05CEST27-Oct-18" +235185 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2509h25ml.pcr F*y% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F*J % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.51ml F*#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +21 h F*,% h %Z ."MFBPCR"\AS.seek 0.81ml F*('> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*T% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*V% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*4  +31 h  ."MFBPCR"\ATV.dial :air3 F*@ % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*d% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*9% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2511h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*|'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*r n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*!  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*\\  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*=  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*A' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*x  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*D'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*H'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.65ml F*C' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*>  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*<'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2511h25ml.pcr F*O% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*n  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*E'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*c  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*f% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*G  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *V'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2513h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*@  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*d I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F**% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*9  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*N'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*q% h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*/ n +21 h n ."MFBPCR"delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F*I' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*?  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*~% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2513h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*"  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*\\'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*='n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*A  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*F'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*<'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *]'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2516h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*L  +11 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*m'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*) n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*c  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*7 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*'' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*y I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*+' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*J % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*o'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*G  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*# n +21 h n ."MFBPCR"delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.65ml F*T' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*k  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2516h25ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *@'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*4'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*_'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*9  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F*} % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*r  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *A'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct2518h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*x  +11 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*.'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*%  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*C n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*U  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*F' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*n  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*<'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*t  +22 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*Z'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*8  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*h' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct2518h25ml.pcr F*1% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*)  +21 h ."MFBPCR"\ATV.dial :air3 F*l % +87 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*J  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*+% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *G'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +32 h  ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *;' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*T% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2520h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*B  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *2'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*}  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *z'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*~ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*g % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*i' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*\ '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*r  +21 h  ."MFBPCR"delay 7 seconds +700 F*6% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*a n +21 h n ."MFBPCR"delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*x  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2520h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*A @@T ."MFBPCR"\ASV.dial :SPEdelivery F*.  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *H' +424 h ' ."MFBPCR"\ATV.dial :ASV F*% n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*>  +42 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*C'\ +2034 h '\  ."MFBPCR"\ATV.dial :waste F*$ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *U'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +42 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :air3 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*<% h % ."MFBPCR"\AS.seek 0.71ml F*^' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h % H ."MFBPCR"\AS.seek 0.735ml F*8' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*Z% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*`% h % zG ."MFBPCR"\AS.seek 0.365ml F*h' +7161 h ' ."MFBPCR"\ATV.dial :waste F*L n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*1 % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*M% h % ."MFBPCR"\AS.seek 0.579ml F*m' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %  ."MFBPCR"\AS.seek 0.604ml F*c'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*f% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*-' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*y n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*+ % +108 h % F*'% h %V ."MFBPCR"\AS.seek 0.01ml F*G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*o% h % ."MFBPCR"\AS.seek 0.58ml F*#' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h % q ."MFBPCR"\AS.seek 0.605ml F*K'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*k% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*(' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*T  +21 h  ."MFBPCR"\AS.seek 0ml F*;'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *V'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*W n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*@ n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*d  +43 h  F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *4' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *P'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*9  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *B': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *q'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*/ n +65 h n F*N% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *2' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*& I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*b  +43 h  F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *I': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*z  +65 h  F*g% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *|'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*? n +64 h n F*"% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Q' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*i I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*6  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*a n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *!'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*\\  +85 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +21 h F*{% h % ."MFBPCR"\AS.seek 0.96ml F*x'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +21 h % ."MFBPCR"delay 3 seconds +300 F*.% h %0 ."MFBPCR"\AS.seek 0.985ml F*3' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*D% h % ."MFBPCR"\AS.seek 0.97ml F*'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*% I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *$'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *X'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *O' +9988 h ' ."MFBPCR"\ATV.dial AIV F*F I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.seek 0ml F*<'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*t% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*]  +22 h ."MFBPCR"\ATV.dial :air3 F*^ % +43 h % F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*h'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*l  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*- % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*G'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F*#'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*:  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' @CEST1540504837.15 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*u % +107 h % ."MFBPCR"\AS.seek 0ml F*@'V +2548 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540613124.33 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )d h  C' '* h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *4  h  .t\SPE.configure SPEconfig F*\ 2 *P% h %V .t\AS.configure ASconfig S)9% F*#,,'T(^20( b h % .t\SS.configure SSconfig C' & S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:08:54CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #23) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&31 +150 h 1 M&%& h &_ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &>(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &$'η +91 h 'η .tHand.close M&C/ +150 h / M&U& h &- M&% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +265 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C't(W +416 h (W C' & h &W .tHand.open M&<1 +150 h 1 M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'S% h %4 .t\CSV.dial CSR C'8  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'Z'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'L'[ +463 h '[ .t\CSV.dial :waste C'` +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +2010 h '4 #t\Purging external Intake C'& h &W C'h% h %4 .t\CSV.dial :flush C'l  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C')  'w h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'['4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'm  +76 h #t\Flushing upper manifold C'7& h &W C'f% h %4 .t\CSV.dial :flush C'c  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'% h %p .t\CS.seek 7ml C'y'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'+ +113 h .t\CTV.dial :air .\CS.seek 0ml C'''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'o'4 +507 h '4 C'& h &W C'e% h %4 .t\CSV.dial :flush C',  +37 h  .t\CS.seek 3ml C'G'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C': @ +37 h @ C'#% h %0 .t\CS.seek 5ml C'k'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C'('4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +415 h ( M&W& h &ӷ M&u% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M&j1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 18:08:58CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:08:54CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*@% h %&K #tLoading sh1 puck from tube 5 into Collection Clamp #"readyMFB"Readying the MFB C'A& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&d% h %\  A(*% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +6 F*4% h %&K ."readyMFB"\ATV.dial :waste2 F*P % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *9' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &; M&_& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &(_ +166 h (_ M&B& h &_ A(/& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &N'e +89 h 'e A(q& h &; .t\SE.seek 20.71 Pucks below Top Plate A(&' +57 h ' A(2& h &; M&}& h &_ .t\Elbow.seek 5 M&b(_ +6 h (_ M&~& h &_ A(g& h &; .t\Forearm.seek 5 + 300 counts M&' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &?/ +150 h / ."downElevator"\SE.seek :down M&z& A("'X h &_ M&|% h % .t\Forearm.seek :retract M&Q'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +91 h ' .t\CC.seek :closed C'p(W +416 h (W .tHand.open M&.1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)% h % C'D& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X9 h 9 ,d +100 C'C9 h 9$0d +100 C'9 h 9<4d +100 C' 9 h 9S8d +100 C'<9 h 9kd +100 C'f9 h 9Td +100 C'9 h 9kd +100 C'c9 h 9d +100 C'[9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'+9 h 9d +100 C'y9 h 9d +100 C''9 h 9 d +100 C'19 h 9 d +100 C'79 h 96d +100 C'J9 h 9Ld +100 C'9 h 9bd +100 C'e9 h 9xd +100 C'-9 h 9d +100 C'o9 h 9d +100 C':9 h 9d +100 C'#9 h 9d +100 C',9 h 9d +100 C'G9 h 9d +100 C'K9 h 9d +100 C'T9 h 9(d +100 C';9 h 9>d +100 C'k9 h 9Td +100 C'V9 h 9id +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'^9 h 9d +100 C't9 h 9d +100 C'u9 h 9  d +100 C' 9 h 9 d +100 C'n9 h 9 *d +100 C'59 h 9 ?d +100 C's9 h 9 Td +100 C'j9 h 9 j d +100 C'W9 h 9 $d +100 C' 9 h 9 (d +100 C'9 h 9 ,d +100 C'@9 h 9 0d +100 C'd9 h 9 4d +100 C'*9 h 9 8d +100 C'9 h 9 % h %p .t\CS.seek 7ml C'C'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +38 h @ .t\CS.seek 10ml C' ' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'< ` +131 h ` #tLysing Complete C']& h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M&E% h %\  A(X% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (v(,% +201 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (r& M&& h &,% ."raiseStack"\SE.seek :upperLimit h &ӷ A(S'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +6 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'l( +23 h 'Z +393 ( A(Z& h &,% A()& h &,% M&w& h &ӷ M&m% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &((k +143 h (k M&f& h &k A(& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &c' +92 h ' .t\Elbow.hold M&[, +7 h ,k .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(+& h &,% .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&y' +21 h ' M&'& h &k A(1& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &7'Ю +22 h 'Ю A(J% h %Z ."downElevator"\SE.seek :down A('X +100 (e% h % M&-% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C': @ + A(6 +18 h @ .t\CS.seek 10ml C'#' +6 h ' .t\CSV.dial between air and CTV C', ` +19 h ` .t\AIV.dial :bypass C'G { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'K& h & .tHand.open M&T1 +2 h 1 M&;& h &k M&k% h %\  .t\Elbow.seek FlushPuck::Garage M&V(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&/ +150 h / M&^& h &- M&t% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'j(W +416 h (W C'W& h &W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%d +150 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%4  +38 h  .t\PS.seek 0ml P%P' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%\\' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'_  h +75 .t\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%B  h .t\PS.seek 0ml P%/' +2609 h ' .t\PSV.dial :air P%q  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%}' +2610 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%Y' +2609 h ' .t\PSV.dial :mixloop P%2 +38 h .t\PS.seek 0ml P%z' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&0& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +6 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'!( +416 h ( M&R& h &ӷ M&6% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +265 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +90 h 'η .tHand.open M&.1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'D& h & M&I% h %\  A(a% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +201 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (& h &; M&=& h &- M&H% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &(_ +166 h (_ M&9& h &_ A(3& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &'e +89 h 'e A(& h &; .t\SE.seek 19.71 Pucks below Top Plate A({' +66 h ' A(i& h &; M&U& h &_ .t\Elbow.seek 5 M&O(_ +7 h (_ M&>& h &_ A(F& h &; .t\Forearm.seek 5 + 300 counts M&' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &%/ +150 h / ."downElevator"\SE.seek :down A(<'X M&C& h &_ M&]% h % .t\Forearm.seek :retract M&E'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&X(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'`(W +416 h (W .tHand.open M&L1 +150 h 1 M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'\  +151 h  .t\CSV.dial CTV C'p  +18 h  .t\CS.seek 5ml C'Z'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C')' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'w'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%m  +38 h  .t\PS.seek 2.5ml P%f' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%8 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'+ @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C''  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'1 @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'J  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'M ` +19 h ` .t\CSV.dial :air C'( @ +38 h @ .t\CS.seek 10ml C'e' +2647 h ' .t\CSV.dial CTV C'-  +37 h  .t\CS.seek 0ml C'c'4 +2648 h '4 .t\CBV.dial :waste1 C'y @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C':  +151 h C'S& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&,% h %\  A(G% h %X A(K. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (T(,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (;& h &,% M&k& h &ӷ M&7% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +143 h (k M&& h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &v'Ю +89 h 'Ю A( & h &,% .t\SE.seek -0.29 Pucks below Top Plate A(['\\ +265 h '\\ M&& h &k A(^& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &t' +25 h ' .t\Elbow.hold M&o, +6 h ,k .t\Elbow.reconfigure TightElbow A( - M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(n' +27 h ' M&5% h % A(V& h &,% .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&u' +21 h ' M&l& h &k A(W& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & 'Ю +22 h 'Ю .t\SE.seek 1.04 Pucks below Top Plate A(' +36 h ' M&d& h &k M&*% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +89 h '\  .t\Elbow.seek CC M&4(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&P' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'_( +416 h ( A(A& h &,% A(B& h &,% M&\\& h &ӷ M&q% h % .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +142 h (k M&& h &k A(~& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &}' +93 h ' .t\Elbow.hold M&2, +6 h ,k .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Y1 +150 h 1 M&0% h % A(g& h &,% .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&|' +20 h ' M&N& h &k A( & h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &@'Ю +23 h 'Ю A(j% h % ."downElevator"\SE.seek :down A(R'X +100 (6% h %S M&% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%Q  + A(6 +112 h  P%?% h % .t\PS.seek 0.499ml P%.' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%D  +37 h .t\CBV.dial AIV C'I  +38 h .t\PRV.dial between CBV and air P%a  +19 h  .t\PRV.dial :air P%  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +250 h ' .t\PRV.dial :waste2 P%H @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %b' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'9& h & .tHand.open M&31 +2 h 1 M&& h &k M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&i(- +166 h (- M&U& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.seek :garage M&F'η +91 h 'η .tHand.close M&/ +150 h / M&%& h &- M&C% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'{& h &W .tHand.open M&<1 +150 h 1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  C'!& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%L @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%h  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%\  +75 h  .tdelay 2 seconds +200 P%p% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%#  +38 h  .t\PS.seek 0ml P%Z' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%) +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%m  +38 h  .tdelay 2 seconds +200 P%z% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%8% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%f  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%+'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%'  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%1  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%M +75 h .t\PTV.dial :PRV,avoiding: :puck P%( @ +76 h @ .t\PS.seek 0ml P%w' +2609 h ' .t\PSV.dial :air P%-  +76 h  .t\PS.seek 2.5ml P%e' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%y +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%:' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%S  +76 h  .t\PS.seek 2.5ml P%,' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%G +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%K  +75 h P%;% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %k' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'7 & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %X' +1313 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P%^ +38 h .t\PS.seek 0ml P% ' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '5& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'V& h &W F*l @@T .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass .\PSV.dial PSR F*W  P%d +22 h  ."MFBPCR"delay 20 seconds +91 h P%*% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%v' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +128 h I F*[% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *t'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%n' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%o' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +23 h ' .t\PSV.dial PSR P%s +20 h  F*`% h %6 ."MFBPCR"\AS.seek 0.329ml F*A'ͯ +92 h P%B% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%\\' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*q % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%~  +38 h  .t\PS.seek 0ml P%P' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*4  +22 h  F*Y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *0' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%g +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%N' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*j% h % ."MFBPCR"\AS.seek 0.469ml F*/' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%6' +2610 h ' .t\PSV.dial PSR P% +113 h P%}% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%|' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%Q' +740 h ' ."MFBPCR"delay 5 seconds +500 F*?9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*@ n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*R% h % ."MFBPCR"\AS.seek 0.041ml F*T'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial PSR P%I +113 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%.' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%H +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%&  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %U' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*> I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *2'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%%' +2609 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %"' +5107 h ' .t\PSV.dial :air P%F  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *]'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*_ I +43 h I F*{% h %Q ."MFBPCR"\AS.seek 0.01ml F*<' +171 h ' .t\PSV.dial :mixloop P%O +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %!' +618 h ' ."MFBPCR"\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.seek :empty F*r'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*h  +85 h  F*\ % h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *p' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*b  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *#'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C')% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'm'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'z' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'Z  +37 h  +113  .t\CBV.dial :waste1 C'8 @ +38 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*f  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*''ݏ +110 h '4 .t\CSV.dial CSR C'1  +38 h  C'+% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'M'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*(  +21 h F*J% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*-'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*w n +108 h n F*y% h %& ."MFBPCR"\AS.seek 0.092ml F*e'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'S' +1626 h ' ."connect"\CSV.dial CTV C':  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'G'4 +2648 h '4 C'K& h &W #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +6 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&k' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&X% h % .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &W'η +91 h 'η .tHand.open M&d1 +150 h 1 M&*% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'& h & M&[% h %\  A(% h %X A($. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (v(; +201 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &; M&& h &- M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &n(_ +166 h (_ M& & h &_ A(`& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &s'e +89 h 'e A(B& h &; ."WCR"\SE.seek 18.71 Pucks below Top Plate A(t' +77 h ' A(o& h &; M&q& h &_ ."WCR"\Elbow.seek 5 M&A(_ +6 h (_ M&~& h &_ A(4& h &; ."WCR"\Forearm.seek 5 + 300 counts M&Y' +23 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &\\/ +150 h / ."downElevator"\SE.seek :down A(g'X M&& h &_ M& % h % ."WCR"\Forearm.seek :retract M&j'\ +27 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&P(ӷ +143 h (ӷ M&0& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &N' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F*} % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +370 h (W ."WCR"Hand.open M&1 +150 h 1 M&|% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*@  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *R' +30 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)/+ +6 h + S)% h % C'Q& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 S)9% h %W S)% h %W ."WCR"Intake.select :close S)0 +6 )U% h %W ."WCR"\SSV.dial :exhaust S)C  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X'\  +1031 h '\  F*9% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*U% h %(A ."MFBPCR"\AS.seek 0.6ml F*C'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*"'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *i' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*2 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +3472 h 'V ."MFBPCR"\SPE.coast F*F  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2709h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*{  +64 h  ."MFBPCR"\ARV.dial :carrier F*O  +11 h  F*=% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*_  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*p  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*)% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*m  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*8 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*1% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*w' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*'  +43 h  ."MFBPCR"\ASV.dial :waste1 F*-  +64 h ."MFBPCR"\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*; % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'7  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'K @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'E ` +19 h ` ."WCR"\CS.seek 7.288ml C'k' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'5  'X  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ','4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'd  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'V' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'^  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'*  +38 h  C'[% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'$' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'W% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'`' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'B  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'o'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'q  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'4  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'~% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'\\' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'n @ +75 h @ +50 S)s% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'Y ` +56 h ` ."WCR"\CS.seek 7.288ml C'0' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'}  'g  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'A'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C't  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'e  +151 h C'j& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&|% h %\  A(@% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (Q(,% +200 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M&& h &ӷ M&6% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &v(k +143 h (k M&N& h &k A(a& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &I'Ю +89 h 'Ю A(/& h &,% ."WCR"\SE.seek 0.71 Pucks below Top Plate A('K +254 h 'K M&D& h &k A(& h &,% ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &T' +25 h ' ."WCR"\Elbow.hold M&., +7 h ,k ."WCR"\Elbow.reconfigure TightElbow A(3- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(&' +26 h ' M&H% h % A(R& h &,% ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&9' +21 h ' M&>& h &k A(U& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +22 h 'Ю ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M&C& h &k M&% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +89 h '\  ."WCR"\Elbow.seek CC M&2(ӷ +143 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ ."WCR"\Forearm.seek CC M&{' +91 h ' ."WCR"Hand.close M&O/ +150 h / ."WCR"\CC.seek :open C'=( +416 h ( A(%& h &,% A(& h &,% M&<& h &ӷ M&L% h % ."WCR"\Forearm.seek :retract M&h'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &_(k +142 h (k M&b& h &k A(& h &,% ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &\ ' +93 h ' ."WCR"\Elbow.hold M&p, +6 h ,k ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&]% h % A(m& h &,% ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&' +20 h ' M&& h &k A(8& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &z'Ю +23 h 'Ю A(c% h % ."downElevator"\SE.seek :down A('X +100 (1% h %B M&Z% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&+1 + A(6 + h 1 M&J& h &k M&(% h %\  .t\Elbow.seek FlushPuck::Garage M&#(- +166 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.seek :garage M&y'η +90 h 'η .tHand.close M&:/ +150 h / M&w& h &- M&M% h %η .t\Forearm.seek :retract M&;'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +265 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'5& h &W C'X% h % .t\CSV.dial CSR C'k  +37 h  C'd% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C','p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '^% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C'[ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'V'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'$& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'B  '  +37 h  +113  .t\CBV.dial :waste1 C'` @ +76 h @ .t\CS.seek 0ml C'q'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'o& h &W C'4% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'~'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'\\% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C's  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'Y& h &W C'% h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C't% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'e 'j  +37 h +76 .t\CTV.dial :air C'|  +150 h .t\CS.seek 0ml C'@'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'6& h &W C'N% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C'/'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'P @ +38 h @ C'?% h %0 .t\CS.seek 5ml C'D'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'3'0 +507 h '0 .t\CSV.dial :waste C'H +75 h .t\CS.seek 0ml C'R'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'>& h &W C'U% h %4 .t\CSV.dial CSR C'C  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'I'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'v' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'T  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'i' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'F  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '9'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'&  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'.'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'O' +710 h ' ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'% @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'<  +37 h  C'L% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +37 h  .t\CBV.dial :waste2b C'2  +76 h  .t\CS.seek 0ml C')'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C']  +38 h  C'm% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +709 h ' ."connect"\CSV.dial CBV C'c .t\CBV.dial CSV C'h  +38 h +75 .t\CTV.dial :air C'_  +151 h .t\CS.seek 0ml C'1'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'+'4 +506 h '4 #t\Flushing upper manifold C'J& h &W C'(% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'z% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CBV C'- .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +506 h '0 .t\CSV.dial :waste C'p +38 h .t\CS.seek 0ml C':'4 +506 h '4 C'w& h &W C'M% h %4 .t\CSV.dial :flush C'f  +38 h  .t\CS.seek 3ml C'y'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +37 h @ C'G% h %0 .t\CS.seek 5ml C'#'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C';'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'5% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.seek 5ml C'k'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'^  ',  +38 h  +113  .t\CBV.dial :waste1 C'[ @ +38 h @ .t\CS.seek 0ml C'*'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'$& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'B  +75 h  .t\CS.seek 0ml C'`'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'o% h %4 .t\CSV.dial CSR C'4  +37 h  C' % h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'q'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ '~  +38 h +75 .t\CTV.dial :intake C's  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C' ' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'n  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Y'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'g% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'0' +1372 h ' .t\CSV.dial CBV C't +113 h .t\CS.seek 0ml C'}'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &e(ӷ +6 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&6& h &ӷ M&N% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &/'η +91 h 'η .tHand.open M&D1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +90 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'3& h & .tHand.open M&>1 +2 h 1 M&U& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &C(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.seek :garage M&I'η +91 h 'η .tHand.close M&/ +150 h / M&T& h &- M&v% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &9' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C'"& h &W .tHand.open M&1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'<& h &W C'L% h %4 .t\CSV.dial CSR C'  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'{'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'] +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'm'4 +2010 h '4 #t\Purging external Intake C')& h &W C'% h %4 .t\CSV.dial :flush C'h  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'_ +76 h .t\CTV.dial :intake C'8  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'1'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'Z& h &W C'J% h %4 .t\CSV.dial :flush C'(  +75 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +38 h @ C'% h %p .t\CS.seek 7ml C'z'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'-'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'p'4 +506 h '4 C'Q& h &W C'w% h %4 .t\CSV.dial :flush C'M  +38 h  .t\CS.seek 3ml C'f'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C': @ +37 h @ C'K% h %0 .t\CS.seek 5ml C'G'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'y'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'#'0 +506 h '0 .t\CSV.dial :waste C'; +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +7 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&d/ +150 h / .t\CC.seek :open C'( +416 h ( M&k& h &ӷ M&^% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &[(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&*% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  #t\Completed [mbaPhase] begun 06:05:39 #\Preparing to sleep until Mon Oct 29 05:05:39 +0100 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 05:05CET29-Oct-18 #\Continuing at 05:05CET29-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:10:23CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 05:05CET29-Oct-18" +235133 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2709h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*B % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +21 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +21 h F*s% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *g'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*7% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*6% h % ."MFBPCR"\AS.seek 0.81ml F*N'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*?'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +21 h F*D% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2711h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*I  +42 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*%  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +22 h ."MFBPCR"delay 2 seconds +200 F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*b  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*2  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*h  +108 h  ."MFBPCR"delay 7 seconds +700 F*m% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +21 h n ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*(' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2711h25ml.pcr F*-% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *M'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*:% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*#% h %n ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*X  +64 h  F*d% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*$  +32 h  ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*l'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*V'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2713h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*g  +10 h  F*Y% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*7  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*6 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*P n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*e' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*D  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*3% h %< ."MFBPCR"\AS.seek 0.594ml F*>'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*T' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*v  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*I% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*H % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2713h25ml.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*F  +22 h ."MFBPCR"\ATV.dial :air3 F*" % +87 h % F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +21 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*)  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +65 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2716h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*O'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *^'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*$  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*, n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.53ml F*l' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*B % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*o  +107 h  ."MFBPCR"delay 7 seconds +700 F*V% h %< ."MFBPCR"\AS.seek 0.594ml F*4'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +21 h n ."MFBPCR"delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*s' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*n  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2716h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*0  +22 h ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +21 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*A'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*D  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +65 h  F*e% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F*T% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct2718h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*m% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.53ml F*z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*'  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*\ '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*: n +21 h n ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*G  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*d'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*i % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct2718h25ml.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*k  +21 h ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *$'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*B  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*l% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *o'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F*q % +86 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*g'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*j'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2720h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ARV.dial :carrier F*@  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*U n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b */'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*I I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*T% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*H % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*%% h %< ."MFBPCR"\AS.seek 0.594ml F*L'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.6ml F*9'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*)% h % \\ ."MFBPCR"\AS.seek 0.326ml F*h'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2720h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*] @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*8  +150 h F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*1 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*+  +43 h  F*Z% h % ."MFBPCR"\AS.seek 0.62ml F*('\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*-'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +423 h ' ."MFBPCR"\ATV.dial :air3 F*z% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.71ml F*:' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h % H ."MFBPCR"\AS.seek 0.735ml F*G' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*f% h % Ű ."MFBPCR"\AS.seek 0.72ml F*#' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*5% h % zG ."MFBPCR"\AS.seek 0.365ml F*;' +7161 h ' ."MFBPCR"\ATV.dial :waste F*= n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*d% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*^% h % ."MFBPCR"\AS.seek 0.579ml F*O' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*$'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*S  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*l % +108 h % F**% h %V ."MFBPCR"\AS.seek 0.01ml F*o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*u' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h % q ."MFBPCR"\AS.seek 0.605ml F*\\'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*~% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*s  +21 h  ."MFBPCR"\AS.seek 0ml F*n'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*g  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *0' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +202 h 'R ."MFBPCR"\SPE.coast F*7  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*|'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*6  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *A' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*P I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*D  +43 h  F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *@': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*?% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *3'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*U n +65 h n F*a% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*>  +43 h  F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *T': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*/  +65 h  F*H% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *C'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*F% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*%  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *<'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*L n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*b  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*9 % +43 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +21 h F*{% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*h' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*_% h % ."MFBPCR"\AS.seek 0.97ml F*c'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*1 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Z'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *J'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *-' +9988 h ' ."MFBPCR"\ATV.dial AIV F*z I +43 h I ."MFBPCR"\AS.seek 0.02ml F*Q' +9880 h ' ."MFBPCR"\ATV.dial :waste F*w n +129 h n ."MFBPCR"\AS.seek 0ml F*\ 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*;'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*d% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*.  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*^  +64 h  F*k% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *E'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F**% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*4% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' @CEST1540677646.89 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*j % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CET1540785939.77 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )6 h  C' '  h  P% %| h  F* *P h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*A  h  .t\SPE.configure SPEconfig F*\ 2 *N% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)D% h % .t\SS.configure SSconfig C'e& S)#.0X% ."rdySampler"\Exhaust.select :close M&I% ."rdySampler"\CTV.dial :air h %q C'v  ."rdyProcessing"\PSV.dial :air h %3 P%/  M&H% h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &T'\ h ."rdySampler"\CSV.dial :waste1 C' h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)F h  S)C  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M&"/ .t\Elbow.jog 1500 M&%* +18 h ."readyCS"\CS.reconfigure CSfast .\CS.home.seek 0ml ."rdySampler"\SS.reconfigure SSconfig C'#d @b ''4 ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:11:19CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #24) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'_& h & .tHand.open M&h1 +150 h 1 M&1& h &_ M&c% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &+(- +166 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&(/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'Q& h &W .tHand.open M&\ 1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'K& h &W C'M% h %4 .t\CSV.dial CSR C'G  +151 h  C'y% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'f'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '#% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '='[ +463 h '[ .t\CSV.dial :waste C'5 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +2009 h '4 #t\Purging external Intake C'd& h &W C';% h %4 .t\CSV.dial :flush C'.  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k 'i  h +75 .t\CTV.dial :intake C'E  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'O  +75 h #t\Flushing upper manifold C',& h &W C'% h %4 .t\CSV.dial :flush C'$  +75 h  .t\CS.seek 5ml C'S'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l +113 h .t\CTV.dial :air .\CS.seek 0ml C'*'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +507 h '0 .t\CSV.dial :waste C'V +37 h .t\CS.seek 0ml C' '4 +507 h '4 C' & h &W C'`% h %4 .t\CSV.dial :flush C'4  +38 h  .t\CS.seek 3ml C'o'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'~'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C's +75 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&n& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'7( +416 h ( M&& h &ӷ M&j% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &[(- +264 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 18:11:24CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:11:19CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 5 into Collection Clamp h %&K C']& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&6% ."readyMFB"\AFV.select :holdingCoil h %\  F* A( % h %X A(|. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (P(; +6 F*A% h %&K ."readyMFB"\ATV.dial :waste2 F*N % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *D' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (e& h &; M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &?(_ +166 h (_ M&@& h &_ A(U& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &a'e +90 h 'e A(3& h &; .t\SE.seek 17.71 Pucks below Top Plate A('* +86 h '* A(& h &; M&R& h &_ .t\Elbow.seek 5 M&>(_ +7 h (_ M&I& h &_ A(H& h &; .t\Forearm.seek 5 + 300 counts M&v' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(/'X M&F& h &_ M&C% h % .t\Forearm.seek :retract M&"'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&L(ӷ +142 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'm(W +416 h (W .tHand.open M&1 +150 h 1 M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)9+ +7 h + S)% h % C'_& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +351 h ' .tdelay 5 seconds +500 .\CSR.select :air C' +6 '"% h % .t\CS.seek 6ml C'7' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C' +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b 'T' +709 h ' .t\CSV.dial CSR C'  +151 h  C'_% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '2' +479 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 'P% h %  .t\CS.seek 4.5ml C''` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'9  +37 h  .t\CS.seek 1.275ml C'c' +920 h ' #tHeating sh1 puck from tube 5 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'1   'Z9 h 9d +100 C'9 h 9d +100 C'}9 h 91d +100 C'D9 h 9J d +100 C'h9 h 9bd +100 C'9 h 9zd +100 C'9 h 9d +100 C'{9 h 9d +100 C'9 h 9 d +100 C'z9 h 9$d +100 C'-9 h 9(d +100 C'+9 h 9 ,d +100 C'(9 h 9$0d +100 C'89 h 9<4d +100 C'Q9 h 9S8d +100 C'\ 9 h 9kd +100 C'9 h 9Td +100 C'E9 h 9kd +100 C'$9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'S9 h 9d +100 C'l9 h 9d +100 C'9 h 9d +100 C'*9 h 9 d +100 C'X9 h 9 d +100 C',9 h 96d +100 C'B9 h 9Ld +100 C' 9 h 9bd +100 C'`9 h 9xd +100 C'9 h 9d +100 C' 9 h 9d +100 C'q9 h 9d +100 C'u9 h 9d +100 C'49 h 9d +100 C'o9 h 9d +100 C'\\9 h 9d +100 C's9 h 9(d +100 C'n9 h 9>d +100 C'~9 h 9Td +100 C'9 h 9id +100 C'V9 h 9d +100 C'g9 h 9d +100 C' 9 h 9d +100 C'09 h 9d +100 C':9 h 9d +100 C'9 h 9d +100 C'j9 h 9  d +100 C't9 h 9 d +100 C''9 h 9 *d +100 C'!9 h 9 ?d +100 C'W9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'Y9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C'69 h 9 4d +100 C' 9 h 9 8d +100 C'|9 h 9 9 h 9 kd +100 C'9 h 9 ~d +100 C'79 h 9 d +100 C'9 h 9 d +100 C'_9 h 9 d .t\CH.hold 5:00,85C C'T   P h  +30000  C'P9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'2'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'9 @ +113 h @ .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 5ml C'Z'p +1373 h 'p .t\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'} @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'D  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'h @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'{  +19 h C'% h %4 .t\CS.seek 5ml C'z'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'-  +38 h C'+% h %p .t\CS.seek 7ml C'('i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'8 @ +38 h @ .t\CS.seek 10ml C'Q' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'\ ` +132 h ` #tLysing Complete C'p& h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M&K% h %\  A(J% h %X A(y. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (w(,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M&#& h &ӷ M&M% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(k +142 h (k M&5& h &k A(=& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &d'Ю +90 h 'Ю A(;& h &,% .t\SE.seek 1.71 Pucks below Top Plate A(.': +244 h ': M&f& h &k A(i& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &k' +25 h ' .t\Elbow.hold M&O, +7 h ,k .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(^- h -+: .t\SE.seek 2.04 Pucks below Top Plate A( ' +27 h ' M&% h % A(E& h &,% .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&$' +21 h ' M&& h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &S'Ю +22 h 'Ю .t\SE.seek 3.04 Pucks below Top Plate A(l' +36 h ' M&& h &k M&*% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  .t\Elbow.seek CC M&,(ӷ +142 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&`' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( A(q& h &,% A(u& h &,% M&4& h &ӷ M&o% h % .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &s(k +142 h (k M&n& h &k A(~& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&V, +6 h ,k .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &g1 +150 h 1 M& % h % A(0& h &,% .t\Forearm.force -20 M&5 .t\Forearm.seek 4 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &:' +21 h ' M&& h &k A(j& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &t'Ю +22 h 'Ю A('% h % ."downElevator"\SE.seek :down A(!'X +100 (W% h %1 M&% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'Y @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'6 { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&|1 +2 h 1 M&A& h &k M&N% h %\  .t\Elbow.seek FlushPuck::Garage M&x(- +165 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&]/ +150 h / M&@& h &- M&U% h %η .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'r(W +416 h (W C'& h &W .tHand.open M&F1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%H +150 h P%C% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%?' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%a  +37 h  .t\PS.seek 0ml P%<' +1598 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'v  h +75 .t\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%"  h .t\PS.seek 0ml P%>' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%7' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%_' +2610 h ' .t\PSV.dial :air P%T  +37 h  .t\PS.seek 2.5ml P%P' +2610 h ' .t\PSV.dial :mixloop P%c +38 h .t\PS.seek 0ml P%9' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&D' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&& h &ӷ M&{% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&81 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'\ & h & M&p% h %\  A(K% h %X A(J. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (y(; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (& h &; M&#& h &- M&M% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &5(_ +166 h (_ M&=& h &_ A(;& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &L'e +90 h 'e A(&& h &; .t\SE.seek 16.71 Pucks below Top Plate A(f'; +95 h '; A(i& h &; M&^& h &_ .t\Elbow.seek 5 M&(_ +7 h (_ M&E& h &_ A(& h &; .t\Forearm.seek 5 + 300 counts M&' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(*'X M&d& h &_ M&G% h % .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.seek CC M&k(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +90 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&S1 +150 h 1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'X  +151 h  .t\CSV.dial CTV C'm  +19 h  .t\CS.seek 5ml C'q'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'u' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'4'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%o  +38 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P%~ +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%`  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%, +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%g  +150 h  .t\PS.seek :empty P% ' +2610 h ' .t\CBV.dial :waste1 C'0 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'j @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C''  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'\\ ` +19 h ` .t\CSV.dial :air C's @ +38 h @ .t\CS.seek 10ml C'W' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'Y'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'6& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M& % h %\  A(|% h %X A(A. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (N(,% +201 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (:& h &,% M&l& h &ӷ M&t% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &b(k +142 h (k M&e& h &k A(w& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &!'Ю +90 h 'Ю A(Q& h &,% .t\SE.seek 2.71 Pucks below Top Plate A(V') +234 h ') M&]& h &k A(@& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &U' +26 h ' .t\Elbow.hold M&, +6 h ,k .t\Elbow.reconfigure TightElbow A(- h -+) M&$&.,(\ #b .t\SE.seek 3.04 Pucks below Top Plate A([' +27 h ' M&R% h % A(x& h &,% .t\Forearm.force -20 .\Forearm.seek 4 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &3' +20 h ' M& & h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &F'Ю +22 h 'Ю .t\SE.seek 4.04 Pucks below Top Plate A(' +37 h ' M&H& h &k M&C% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  .t\Elbow.seek CC M&a(ӷ +142 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( A(/& h &,% A("& h &,% M&%& h &ӷ M&% h % .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +143 h (k M&7& h &k A(T& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &_' +92 h ' .t\Elbow.hold M&c, +7 h ,k .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &P1 +150 h 1 M&% h % A(& h &,% .t\Forearm.force -20 M&5 .t\Forearm.seek 4 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &}' +21 h ' M&h& h &k A(& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'Ю +22 h 'Ю A(r% h % ."downElevator"\SE.seek :down A('X +100 ({% h %  M&D% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +90 h '\  +31 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%+  + A(6 +112 h  P%2% h % .t\PS.seek 0.499ml P%8' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%1 +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%\  +37 h .t\CBV.dial AIV C'p  +38 h .t\PRV.dial between CBV and air P%K  +19 h  .t\PRV.dial :air P%J  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %#' +250 h ' .t\PRV.dial :waste2 P%M @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %z' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'=& h & .tHand.open M&;1 +2 h 1 M&&& h &k M&% h %\  .t\Elbow.seek FlushPuck::Garage M&i(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&(/ +150 h / M&& h &- M&d% h %η .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'f(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  C'Z& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%S @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%O  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%X  +75 h  .tdelay 2 seconds +200 P%m% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%y' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%q' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%u +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%o  +38 h  .tdelay 2 seconds +200 P%9% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %~'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%`  +75 h .tdelay 2 seconds +200 P%,% h %r .t\PS.seek 0.475ml P%g' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%n  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%0'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%j  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %'' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck P%s @ +76 h @ .t\PS.seek 0ml P%4' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%W' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%6  +76 h  .t\PS.seek 2.5ml P% ' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%| +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%A  +75 h P%:% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %l' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C't & +50 h & .t\AWV.select :waste C' +7 P%e% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %w'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'Q { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %k' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :PML P%@ +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'R& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'x& h &W F* @@T ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%H +22 h  ."MFBPCR"delay 20 seconds +91 h P%C% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%!' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*b I +128 h I F*V% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *U'6 +66 .t\PSV.dial :waste1 P%?  +38 h  .t\PS.seek 0ml P%)' +1597 h ' .t\PSV.dial PSR P%3 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%[' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%I +21 h  F* % h %6 ."MFBPCR"\AS.seek 0.329ml F*/'ͯ +92 h P%"% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *7'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%T  +38 h  .t\PS.seek 0ml P%<' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*a  +22 h  F*P% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%h' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*r% h % ."MFBPCR"\AS.seek 0.469ml F*>' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%{' +2610 h ' .t\PSV.dial PSR P%D +113 h P%_% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%}' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%+' +740 h ' ."MFBPCR"delay 5 seconds +500 F*29 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*N'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%1' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%\ ' +2610 h ' .t\PSV.dial PSR P%p +113 h P%K% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%8' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%M +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%#' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%; +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %&'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %^' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*E I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *c'V +850 h ' .t\PSV.dial :air P%(  +113 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%d +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %-' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %L' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*B  +43 h  F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *G'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*v I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%* +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %Z' +618 h ' ."MFBPCR"\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*O  +85 h  F*X% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *m' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*z  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'y  +38 h  C'u% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'o'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'9' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'q  '`  +37 h  +113  .t\CBV.dial :waste1 C', @ +38 h @ .t\CS.seek 0ml C'~'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*n  +21 h ."MFBPCR"delay 3 seconds +300 F*g% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'j  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'\\'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*s  +21 h F*'% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*4 n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*W'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'6' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'|'4 +2648 h '4 C'A& h &W #tGaraging FlushPuck in Collection Clamp M&:& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'w( +416 h ( M&& h &ӷ M&k% h % .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &](- +264 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&H1 +150 h 1 M&C% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'b& h & M&V% h %\  A(?% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (!(; +201 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (3& h &; M&)& h &- M&F% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &[(_ +165 h (_ M&& h &_ A( & h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &I'e +90 h 'e A("& h &; ."WCR"\SE.seek 15.71 Pucks below Top Plate A(U'L +106 h 'L A(& h &; M&& h &_ ."WCR"\Elbow.seek 5 M&/(_ +7 h (_ M&T& h &_ A(a& h &; ."WCR"\Forearm.seek 5 + 300 counts M&P' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &%/ +148 h '&0 ."MFBPCR"delay 3 seconds +2 h / ."downElevator"\SE.seek :down M&& A(7'X h &_ M&% h % ."WCR"\Forearm.seek :retract M&r'\ +91 h '\  ."WCR"\Elbow.seek CC M&<(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +64 ."MFBPCR"\ATV.dial :waste2 F*D % +27 h ' ."WCR"\CC.seek :closed C'_(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +399 h (W ."WCR"Hand.open M&1 +150 h 1 M&}% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +11 h 'R ."MFBPCR"\ATV.dial :air2 F*  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)>+ +7 h + S) % h % C'+& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*p  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *J'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*1 @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*N I +86 h I ."MFBPCR"\AS.seek 0ml F*;'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *E'\  +1031 h '\  F*=% h %\  ."MFBPCR"\AS.seek 0.5ml F*('(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*^% h %(A ."MFBPCR"\AS.seek 0.6ml F*d'\  +1031 h '\  F*&% h %\  ."MFBPCR"\AS.seek 0.5ml F*-'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*B% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *i' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*c % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2908h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*L'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*v  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +22 h ."MFBPCR"delay 2 seconds +200 F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*m  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*u% h %Fj ."MFBPCR"\AS.seek 0.524ml F*y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*o  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*, % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F*`'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*g  +107 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +21 h n ."MFBPCR"delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*4' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254572 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C't  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'A @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +910 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)e% h % ."WCR"\CBV.dial between CSV and waste2b C'f  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'. ` +19 h ` ."WCR"\CS.seek 7.288ml C'l' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'R  ."WCR"\CSV.dial CTV C'k  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'H  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'x' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'@  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'C  +38 h  C'V% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'b @ +75 h @ +50 S)?% h % ."WCR"\CBV.dial between CSV and waste2b C'F  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C') ` +57 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'"  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ']' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'a  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'%' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'[ @ +76 h @ +50 S)I% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'P ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'/  'D  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '7'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'U  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'w' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +151 h C'r& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&}% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (+(,% +200 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M& & h &ӷ M&{% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &!(k +142 h (k M&h& h &k A(K& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &p'Ю +90 h 'Ю A(>& h &,% ."WCR"\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' M&\ & h &k A(J& h &,% ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &N' +25 h ' ."WCR"\Elbow.hold M&8, +6 h ,k ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(;- h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(' +27 h ' M&M% h % A(& h &,% ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 4 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &=' +21 h ' M&E& h &k A(^& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &('Ю +22 h 'Ю ."WCR"\SE.seek 5.04 Pucks below Top Plate A(&' +36 h ' M&d& h &k M&B% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +89 h '\  ."WCR"\Elbow.seek CC M&c(ӷ +143 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&*/ +150 h / ."WCR"\CC.seek :open C'#( +416 h ( A(& h &,% A(L& h &,% M&& h &ӷ M&S% h % ."WCR"\Forearm.seek :retract M&O'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &v(k +142 h (k M&z& h &k A($& h &,% ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &X' +93 h ' ."WCR"\Elbow.hold M&m, +6 h ,k ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &u1 +150 h 1 M&G% h % A(o& h &,% ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&q' +20 h ' M&y& h &k A(,& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &9'Ю +23 h 'Ю A(Y% h % ."downElevator"\SE.seek :down A(g'X +100 (j% h % M&`% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +89 h '\  +22 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'~& h & .tHand.open M&01 + A(6 + h 1 M&'& h &k M&s% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&4& h &- M&\\% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'e(W +416 h (W C'f& h &W .tHand.open M&1 +150 h 1 M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'k& h &W C'R% h % .t\CSV.dial CSR C'l  +38 h  C'H% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '@% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'C'[ +463 h '[ .t\CSV.dial :waste C'V +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'x'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'b% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C')% h %p .t\CS.seek 7ml C'3'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '"  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'a% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'T'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'%% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'I  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'P& h &W C'% h %4 .t\CSV.dial :flush C'D  +75 h  .t\CS.seek 5ml C'/'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'U% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV C'W .t\CBV.dial CSV C'r  +38 h +75 .t\CTV.dial :air C'}  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'{& h &W C'h% h %4 .t\CSV.dial :flush C'K  +38 h  .t\CS.seek 3ml C'>'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'2% h %0 .t\CS.seek 5ml C'\ 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'J'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C';'0 +507 h '0 .t\CSV.dial :waste C'M +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'E& h &W C'^% h %4 .t\CSV.dial CSR C'd  +150 h  C'B% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'p'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'N  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'i' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '='4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'8'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +710 h ' ."connect"\CSV.dial CTV C'-  .t\CTV.dial CSV C'_  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'L'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '$' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'c  +75 h  .t\CS.seek 0ml C'u'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'G  +37 h  C'o% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'y'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Y 'O  +37 h +75 .t\CTV.dial :air C'v  +151 h .t\CS.seek 0ml C'j'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'`'0 +506 h '0 .t\CSV.dial :waste C'~ +38 h .t\CS.seek 0ml C'0'4 +507 h '4 #t\Flushing upper manifold C''& h &W C's% h %4 .t\CSV.dial :flush C'X  +37 h  .t\CS.seek 5ml C'q'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'9 +113 h .t\CTV.dial :air .\CS.seek 0ml C'g'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'm +37 h .t\CS.seek 0ml C''4 +507 h '4 C'4& h &W C'\\% h %4 .t\CSV.dial :flush C'n  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +38 h @ C'|% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C':'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'#'0 +506 h '0 .t\CSV.dial :waste C'f +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'.& h &W C'k% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C'l'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C'5% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV C'@  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'V @ +38 h @ .t\CS.seek 0ml C'C'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'b% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'x'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C')% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'"  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #tDisinfecting external Intake with kill C'3& h &W C'% h %4 .t\CSV.dial CSR C'a  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C']' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'%  'T +38 h +75 .t\CTV.dial :intake C'I  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'[  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'P'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'D% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'U +113 h .t\CS.seek 0ml C'/'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&r& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +7 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&{& h &ӷ M&h% h % .t\Forearm.seek :retract M&K'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &>'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M&J% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C';& h & .tHand.open M&E1 +2 h 1 M&^& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &d(- +6 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&p'η +91 h 'η .tHand.close M&/ +150 h / M&N& h &- M&!% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +90 h ' .t\CC.seek :closed C'8(W +416 h (W C'-& h &W .tHand.open M&_1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'S% h %4 .t\CSV.dial CSR C'L  +113 h  C'z% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'c% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '$'[ +463 h '[ .t\CSV.dial :waste C'G +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'o'4 +2009 h '4 #t\Purging external Intake C'u& h &W C'y% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'v +75 h .t\CTV.dial :intake C',  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'j'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'~  +75 h #t\Flushing upper manifold C'`& h &W C''% h %4 .t\CSV.dial :flush C's  +75 h  .t\CS.seek 5ml C'X'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'&% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'q +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'g +37 h .t\CS.seek 0ml C'm'4 +507 h '4 C'+& h &W C'4% h %4 .t\CSV.dial :flush C'\\  +37 h  .t\CS.seek 3ml C'n'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'A% h %0 .t\CS.seek 5ml C'|'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C': +76 h .t\CS.seek 0ml C'f'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&#& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +7 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&H/ +150 h / .t\CC.seek :open C'5( +416 h ( M&l& h &ӷ M&@% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +91 h 'η .tHand.open M&?1 +150 h 1 M&C% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 05:05:55 #\Preparing to sleep until Wed Oct 31 05:05:55 +0100 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 05:05CET31-Oct-18 #\Continuing at 05:05CET31-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeploy.rb:125\\n{1 => 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:12:50CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 05:05CET31-Oct-18" +235163 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2908h25ml.pcr F*)% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*" % +87 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*%'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*[  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*7  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*t% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*{% h % ."MFBPCR"\AS.seek 0.81ml F*h'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +21 h F*\ % h %m ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2910h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*p  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*d'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *-'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*z  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*=% h %Fj ."MFBPCR"\AS.seek 0.524ml F*/' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*c  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*G' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*$  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +108 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*v'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*, n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*~'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*`  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*s' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*j  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*0  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2910h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*+ % +86 h % F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*A'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*#'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +65 h  F*H% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*b% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*x'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2912h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ARV.dial :carrier F*D  +10 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +22 h ."MFBPCR"delay 2 seconds +200 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *e'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*{ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*Q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*< n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.53ml F*W' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*K'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +107 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*E'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*p% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*M % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2912h25ml.pcr F*d% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*- % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +21 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*$'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +65 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *o'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +31 h  ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*q% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*g  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2915h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *w'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*b% h %Fj ."MFBPCR"\AS.seek 0.524ml F*C' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*) I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.53ml F* ' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*" % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*I' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*]'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*P % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2915h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*}% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*e'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*h% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*2  +65 h  F*W% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*!% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*N% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*-% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct2917h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*O  +43 h  ."MFBPCR"\ARV.dial :carrier F*$  +11 h  F*o% h %V ."MFBPCR"\AS.seek 0.5ml F*y'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*~  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*j  +22 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *''Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*s n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*q% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*1  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*g  +22 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*m'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*i % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct2917h25ml.pcr F*R% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*l  +21 h ."MFBPCR"\ATV.dial :air3 F*8 % +87 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*?% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*6% h %n ."MFBPCR"\AS.seek 0.82ml F*C'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*F  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*T  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*]'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*U% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2919h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*;'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*^ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*p I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*M % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*(' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*='\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*L  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*c  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*u% h % \\ ."MFBPCR"\AS.seek 0.326ml F*O'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*o % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2919h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*G @@T ."MFBPCR"\ASV.dial :SPEdelivery F*y  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*,  +150 h F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *~' +424 h ' ."MFBPCR"\ATV.dial :ASV F*j n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*0  +43 h  F*`% h % ."MFBPCR"\AS.seek 0.62ml F*s'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*q n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *1'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*9'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*& % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*X% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h % H ."MFBPCR"\AS.seek 0.735ml F*|' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*n% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*k% h % zG ."MFBPCR"\AS.seek 0.365ml F*:' +7161 h ' ."MFBPCR"\ATV.dial :waste F*# n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*@% h % ."MFBPCR"\AS.seek 0.579ml F**' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*b% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*) n +86 h n ."MFBPCR"\ASV.dial :wasteR F*6  +21 h  ."MFBPCR"\AS.seek 0ml F*w'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *F'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*C% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*3' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h % q ."MFBPCR"\AS.seek 0.605ml F*T'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*%% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*I  +21 h  ."MFBPCR"\AS.seek 0ml F*['V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*D  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*U n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *]'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *r'R +202 h 'R ."MFBPCR"\SPE.coast F*t  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*{  +43 h  F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *7' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*< I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\  +43 h  F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*J  +65 h  F*2% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *;'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*^ n +65 h n F*K% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *B' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*E  +43 h  F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *N': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*>  +65 h  F*M% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *d'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *-' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*( I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*_ n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*S n +151 h n ."MFBPCR"\AS.seek 0ml F*L'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*z  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*= % +42 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *c' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*$'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %0 ."MFBPCR"\AS.seek 0.985ml F*O' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*v% h % ."MFBPCR"\AS.seek 0.97ml F*Y'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*j I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *`'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *q'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *''R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*1  +43 h  F*&% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *9' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*+' +9880 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek 0ml F*X'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*g% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *n'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*:'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*8  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*@  +65 h  F*l% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *.'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*F'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *%'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*t n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *]'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*r % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CET1540958755.21 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ){ h  C' 'Q h  P% %} h  F* *< h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *7  h  .t\SPE.configure SPEconfig F*\ 2 *h% h %V .t\AS.configure ASconfig S)\ % F*#,,'T(^20( b h % .t\SS.configure SSconfig C'W& h & .t\CC.configure CCconfig S)#.0X  h %3 .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &N'\ h ."rdySampler"\CSV.dial :waste1 C' h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S) h  S)d  +37 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %(' +21 h '\  ."closeHand"Hand.close .t\Elbow.jog 1500 M&-* + &/ +16 h ."readyCS"\CS.reconfigure CSfast .\CS.home.seek 0ml ."rdySampler"\SS.reconfigure SSconfig C'#d @b ''4 ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:13:49CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeploy phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #25) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'v& h & .tHand.open M&O1 +150 h 1 M&j& h &_ M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &0(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &q'η +91 h 'η .tHand.close M&s/ +150 h / M&1& h &- M&&% h %η .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &4' +90 h ' .t\CC.seek :closed C'g(W +416 h (W C'+& h &W .tHand.open M&X1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'\\% h %4 .t\CSV.dial CSR C'|  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'n'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '#'[ +463 h '[ .t\CSV.dial :waste C'k +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +2010 h '4 #t\Purging external Intake C'H& h &W C':% h %4 .t\CSV.dial :flush C'8  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l 'i  h +76 .t\CTV.dial :intake C'.  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'?'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'*  +75 h #t\Flushing upper manifold C' & h &W C'b% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'6'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'w% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'C'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'"'0 +506 h '0 .t\CSV.dial :waste C'x +38 h .t\CS.seek 0ml C''4 +506 h '4 C'F& h &W C' % h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'3% h %0 .t\CS.seek 5ml C'%'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'T'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'I +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +6 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&U' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C't( +416 h ( M&& h &ӷ M&r% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&1 +150 h 1 M&Z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +90 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 18:13:54CEST30-Aug-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:13:49CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 5 into Collection Clamp h %&K C'G& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&{% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(Q% h %X A(}. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (<(; +7 F*7% h %&K ."readyMFB"\ATV.dial :waste2 F*h % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *\ ' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (W& h &; M&e& h &- M&J% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &2(_ +166 h (_ M&& h &_ A(^& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &K'e +89 h 'e A(;& h &; .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(B& h &; M&& h &_ .t\Elbow.seek 5 M&E(_ +7 h (_ M&p& h &_ A(!& h &; .t\Forearm.seek 5 + 300 counts M&M' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&>& A('X h &_ M&d% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&S(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'o(W +416 h (W .tHand.open M&y1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +7 h + S)L% h % C'v& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'b9 h 9Td +100 C'.9 h 9kd +100 C'9 h 9d +100 C'?9 h 9d +100 C'w9 h 9d +100 C'69 h 9d +100 C' 9 h 9d +100 C')9 h 9d +100 C'C9 h 9 d +100 C'R9 h 9 d +100 C' 9 h 96d +100 C'"9 h 9Ld +100 C'F9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'39 h 9d +100 C'a9 h 9d +100 C'9 h 9d +100 C'T9 h 9d +100 C'I9 h 9(d +100 C'[9 h 9>d +100 C'%9 h 9Td +100 C'9 h 9id +100 C'x9 h 9d +100 C'D9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'g9 h 9d +100 C'r9 h 9  d +100 C'V9 h 9 d +100 C'9 h 9 *d +100 C'U9 h 9 ?d +100 C'f9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'P9 h 9 (d +100 C']9 h 9 ,d +100 C'9 h 9 0d +100 C'{9 h 9 4d +100 C'Q9 h 9 8d +100 C'}9 h 9 9 h 9 Wd +100 C'59 h 9 kd +100 C' 9 h 9 d +100 C'!9 h 9 d +100 C'd9 h 9 d +100 C'29 h 9 d +100 C'K9 h 9 d +100 C'9 h 9 d +100 C'u9 h 9 d +100 C'-9 h 9 d +100 C'M9 h 9 d +100 C'9 h 9 1d +100 C'E9 h 9 Dd +100 C'9 h 9 Xd +100 C'9 h 9 kd +100 C'y9 h 9 ~d +100 C't9 h 9 d +100 C'L9 h 9 d +100 C'v9 h 9 d .t\CH.hold 5:00,85C C'N   P h  +30000  C'_9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'c'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'= @ +113 h @ .t\CSV.dial :air C'< @ +37 h @ .t\CS.seek 5ml C'`'p +1373 h 'p .t\CSV.dial CTV C'~  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'/  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'O @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'1'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'& +113 h .t\CBV.dial CSV C'  +19 h C'(% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'9  +38 h C'0% h %p .t\CS.seek 7ml C's'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C', @ +37 h @ .t\CS.seek 10ml C'+' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'X ` +132 h ` #tLysing Complete C'm& h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M&A% h %\  A('% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (4(,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M&& h &ӷ M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &|(k +142 h (k M&k& h &k A(#& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &H'Ю +90 h 'Ю A(:& h &,% .t\SE.seek 4.71 Pucks below Top Plate A(8' +214 h ' M&n& h &k A(i& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &l' +26 h ' .t\Elbow.hold M&*, +6 h ,k .t\Elbow.reconfigure TightElbow A(@- M&$&.,(\ #b h -+ .t\SE.seek 5.04 Pucks below Top Plate A(' +27 h ' M&b% h % A(.& h &,% .t\Forearm.force -20 M&5 .t\Forearm.seek 4 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &' +20 h ' M&?& h &k A(w& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &6'Ю +22 h 'Ю .t\SE.seek 6.04 Pucks below Top Plate A( ' +37 h ' M&)& h &k M&C% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +89 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &,% A(3& h &,% M&a& h &ӷ M&% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &I(k +143 h (k M&[& h &k A(%& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&x, +7 h ,k .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &D1 +150 h 1 M&% h % A(& h &,% .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&' +21 h ' M&g& h &k A(r& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &V'Ю +22 h 'Ю A(% h % ."downElevator"\SE.seek :down A(U'X +100 (f% h % M&% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +12 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'P @ +2 A(6 +17 h @ .t\CS.seek 10ml C']' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'{ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Q& h & .tHand.open M&}1 +2 h 1 M&7& h &k M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&Z(- +166 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &- .t\Forearm.seek :garage M&e'η +91 h 'η .tHand.close M&G/ +150 h / M&& h &- M&^% h %η .t\Forearm.seek :retract M&;'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'B& h &W .tHand.open M&>1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%! +151 h P%d% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%K  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%-' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%M  C'  h +75 .t\PSV.dial :mixloop P%E +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%y  +37 h  .t\PS.seek 2.5ml P%t' +2610 h ' .t\PSV.dial :mixloop P%L +38 h .t\PS.seek 0ml P%v' +2610 h ' .t\PSV.dial :air P%N  +37 h  .t\PS.seek 2.5ml P%_' +2610 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.seek 0ml P%=' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&/' +91 h ' .tHand.close M&O/ +150 h / .t\CC.seek :open C'`( +416 h ( M&&& h &ӷ M&% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&Y% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'X& h & M&m% h %\  A(A% h %X A('. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (& h &; M&& h &- M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &k(_ +166 h (_ M&#& h &_ A(:& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &S'e +89 h 'e A(& h &; .t\SE.seek 13.71 Pucks below Top Plate A(n'n +126 h 'n A(i& h &; M&@& h &_ .t\Elbow.seek 5 M&b(_ +6 h (_ M&.& h &_ A(?& h &; .t\Forearm.seek 5 + 300 counts M&w' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &)/ +150 h / ."downElevator"\SE.seek :down M&C& A(H'X h &_ M&|% h % .t\Forearm.seek :retract M&8'\ +90 h '\  .t\Elbow.seek CC M&l(ӷ +35 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&61 +150 h 1 M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'R  +150 h  .t\CSV.dial CTV C'o  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'3' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'a'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%[' +2610 h ' .t\PSV.dial :mixloop P%% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%D  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'g  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'r @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'T ` +19 h ` .t\CSV.dial :air C'I @ +37 h @ .t\CS.seek 10ml C'f' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'P'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'{  +151 h C'Q& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&}% h %\  A(7% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,% +200 h (,% .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,% M& & h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &z(k +143 h (k M&W& h &k A(4& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &U'Ю +89 h 'Ю A(+& h &,% .t\SE.seek 5.71 Pucks below Top Plate A(x' +205 h ' M&G& h &k A(& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 4 M&$ ,(#b &#Q@2^d^2b &^' +25 h ' .t\Elbow.hold M&, +7 h ,k .t\Elbow.reconfigure TightElbow A(e- M&$&.,(\ #b h -+ .t\SE.seek 6.04 Pucks below Top Plate A(' +27 h ' M&% h % A(Z& h &,% .t\Forearm.force -20 M&5 .t\Forearm.seek 4 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &;' +21 h ' M&& h &k A(B& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &>'Ю +22 h 'Ю .t\SE.seek 7.04 Pucks below Top Plate A(5' +36 h ' M&!& h &k M&d% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +90 h '\  .t\Elbow.seek CC M&K(ӷ +142 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&M/ +150 h / .t\CC.seek :open C'( +416 h ( A(E& h &,% A(& h &,% M&-& h &ӷ M&y% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &L(k +143 h (k M&t& h &k A(N& h &,% .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &v' +92 h ' .t\Elbow.hold M&j, +7 h ,k .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &_1 +150 h 1 M&<% h % A(~& h &,% .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&\ ' +21 h ' M&O& h &k A(J& h &,% .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & 'Ю +22 h 'Ю A( % h % ."downElevator"\SE.seek :down A(&'X +100 (% h % M&/% h %Ю .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%0  + A(6 +112 h  P%c% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%Y +37 h .t\PRV.dial :air P%(  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%X  +37 h .t\CBV.dial AIV C'm  +38 h .t\PRV.dial between CBV and air P%A  +19 h  .t\PRV.dial :air P%'  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%\\ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'#& h & .tHand.open M&:1 +2 h 1 M&& h &k M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&i(- +166 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.seek :garage M&?'η +91 h 'η .tHand.close M&s/ +150 h / M&)& h &- M&C% h %η .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +264 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'w(W +416 h (W C'n& h &W .tHand.open M&b1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  C'`& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%6 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%*  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%R  +75 h  .tdelay 2 seconds +200 P%o% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%]  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%3 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%=% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %%'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P% % h %r .t\PS.seek 0.475ml P%D' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%[  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%g  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%r  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%T +75 h .t\PTV.dial :PRV,avoiding: :puck P%I @ +76 h @ .t\PS.seek 0ml P%a' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%f' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%{' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%Q  +76 h  .t\PS.seek 2.5ml P%}' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%7 +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%h  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb % ' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%W% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %4'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'+ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %l' +1313 h ' .t\PSV.dial :air P%G  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%e' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Z& F* @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*B  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%! +22 h  ."MFBPCR"delay 20 seconds +91 h P%d% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%U' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*z I +128 h I F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *^'6 +66 .t\PSV.dial :waste1 P%2  +38 h  .t\PS.seek 0ml P%u' +1597 h ' .t\PSV.dial PSR P%; +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%> +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*M  +23 h ' .t\PSV.dial PSR P%p +20 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*E'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%-' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*y % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%N  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*K  +22 h  F*_% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *<' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%~ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%O' +217 ."MFBPCR"\ATV.dial :AIV F*J I +43 h I F* % h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%L +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%/ +113 h P%v% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%\ ' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +37 h  .t\PS.seek 0ml P%0' +740 h ' ."MFBPCR"delay 5 seconds +500 F*c9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*&% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%( +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial PSR P%m +113 h P%A% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%'' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%\\ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%1  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %@' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*. I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *j'V +850 h ' .t\PSV.dial :air P%s  +113 h  .t\PS.seek 2.5ml P%)' +2609 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %9' +5107 h ' .t\PSV.dial :air P%?  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %S' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*"  +43 h  F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *|'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*n% h %Q ."MFBPCR"\AS.seek 0.01ml F*b' +171 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %`' +618 h ' ."MFBPCR"\ATV.dial :waste F*6 n +129 h n ."MFBPCR"\AS.seek :empty F*F'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F**  +85 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *o' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *]'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'3% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'=' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'%'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*[  +21 h ."MFBPCR"delay 3 seconds +300 F*D% h %_ ."MFBPCR"\AS.seek 0.434ml F*g'ݏ +110 h '4 .t\CSV.dial CSR C'r  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'T'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*I  +21 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*a n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*f'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'Q' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'7'4 +2648 h '4 C'h& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&+/ +150 h / .t\CC.seek :open C'4( +416 h ( M&G& h &ӷ M&l% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&!1 +150 h 1 M&d% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'z& h & M&x% h %\  A(2% h %X A(q. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (U(; +201 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (;& h &; M&u& h &- M&>% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &(_ +166 h (_ M&M& h &_ A(& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &p'e +89 h 'e A(& h &; ."WCR"\SE.seek 12.71 Pucks below Top Plate A(^' +136 h ' A(& h &; M&y& h &_ ."WCR"\Elbow.seek 5 M&E(_ +7 h (_ M&N& h &_ A(K& h &; ."WCR"\Forearm.seek 5 + 300 counts M&_' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &-/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down A(~'X M&t& h &_ M&J% h % ."WCR"\Forearm.seek :retract M& '\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&<& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +35 ."MFBPCR"\ATV.dial :waste2 F*/ % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +13 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&51 +131 h 'R ."MFBPCR"\ATV.dial :air2 F*\  +19 h 1 M&c% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *&' +89 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'0& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'(  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +22 h F*B% h % ."MFBPCR"\AS.seek 0.579ml F*M' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*N' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +86 h n ."MFBPCR"\ASV.dial :wasteR F*e  +43 h  ."MFBPCR"\AS.seek 0ml F*-'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *J'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*p% h %V ."MFBPCR"\AS.seek 0.59ml F*<' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*L n +107 h n ."MFBPCR"\ASV.dial :wasteR F*^% h %  ."MFBPCR"\AS.seek 0.09ml F*_' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*E I +86 h I F*~% h % ."MFBPCR"\AS.seek 0.12ml F* ' +625 h ' ."MFBPCR"\ATV.dial :air3 F*f % +21 h % F*4% h % ."MFBPCR"\AS.seek 0.13ml F*5' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*\ % h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h % ."MFBPCR"\AS.seek 0.255ml F*O'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +21 h F*U% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*A n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*m  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *''5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*Y @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*:'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*&'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*1  +43 h  #"MFBPCR"\Swish #1 of 2 F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *.'\  +1031 h '\  F*#% h %\  ."MFBPCR"\AS.seek 0.5ml F*s'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*@% h %(A ."MFBPCR"\AS.seek 0.6ml F*C'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*9'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*"% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *i' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*j % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +3472 h 'V ."MFBPCR"\SPE.coast F*?  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct3108h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*n  +64 h  ."MFBPCR"\ARV.dial :carrier F*H  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *|'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*o  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*3% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*P' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +107 h  ."MFBPCR"delay 7 seconds +700 F*r% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*[ n +21 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*]'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254602 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'h @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '+' +909 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'k @ +76 h @ +50 S)W% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C'G% h % ."WCR"\CSV.dial between air and CTV C'8 ` +19 h ` ."WCR"\CS.seek 7.288ml C' ' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'l  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '}'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'!  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Z' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'd  +37 h  C'x% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'q' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ';' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'z @ +75 h @ +50 S)2% h % ."WCR"\CBV.dial between CSV and waste2b C'>  +94 h  C'B% h % ."WCR"\CSV.dial between air and CTV C'u ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'M  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'y  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'e  +38 h  C'N% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'-' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)p% h % ."WCR"\CBV.dial between CSV and waste2b C'J  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'^ ` +57 h ` ."WCR"\CS.seek 7.288ml C'<' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'E  '/  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'_  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'f' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'4  +150 h C' & h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&c% h %\  A(\ % h %X A(5. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (0(,% +201 h (,% ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &,% M&& h &ӷ M&v% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &U(k +142 h (k M&O& h &k A(A& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &m'Ю +90 h 'Ю A(& h &,% ."WCR"\SE.seek 6.71 Pucks below Top Plate A(' +195 h ' M&X& h &k A('& h &,% ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &' +26 h ' ."WCR"\Elbow.hold M&,, +6 h ,k ."WCR"\Elbow.reconfigure TightElbow A(:- M&$&.,(\ #b h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(1' +27 h ' M&\\% h % A(&& h &,% ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&#' +20 h ' M&.& h &k A(@& h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &s'Ю +22 h 'Ю ."WCR"\SE.seek 8.04 Pucks below Top Plate A('w +37 h 'w M&C& h &k M&"% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +89 h '\  ."WCR"\Elbow.seek CC M&j(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ ."WCR"\Forearm.seek CC M&n' +91 h ' ."WCR"Hand.close M&H/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A()& h &,% A(S& h &,% M&b& h &ӷ M&6% h % ."WCR"\Forearm.seek :retract M&*'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(k +143 h (k M&& h &k A(& h &,% ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &R' +92 h ' ."WCR"\Elbow.hold M&o, +7 h ,k ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &31 +150 h 1 M&|% h % A(& h &,% ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts + M& ' +21 h ' M&& h &k A( & h &,% ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &='Ю +22 h 'Ю A(P% h %w ."downElevator"\SE.seek :down A(D'X +100 (r% h % M&% h %Ю ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'%& h & .tHand.open M&1 +2 h 1 M&& h &k M&I% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +90 h 'η .tHand.close M&{/ +150 h / M&a& h &- M&T% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +265 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +91 h ' .t\CC.seek :closed C'W(W +416 h (W C'w& h &W .tHand.open M&G1 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'l& h &W C'% h % .t\CSV.dial CSR C'  +37 h  C'!% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'}'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'd'[ +463 h '[ .t\CSV.dial :waste C'x +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'q& h &W C'z% h %4 .t\CSV.dial :flush C'2  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +38 h @ C'u% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'M  +37 h  +113  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'y'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'K% h %4 .t\CSV.dial :flush C'e  +75 h  .t\CS.seek 5ml C'N'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'-% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'J  +76 h  .t\CS.seek 0ml C'L'4 +766 h '4 #t\Flushing upper manifold C'^& h &W C't% h %4 .t\CSV.dial :flush C'/  +75 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'_% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV C'4 .t\CBV.dial CSV C'  +37 h +76 .t\CTV.dial :air C'c  +150 h .t\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +507 h '0 .t\CSV.dial :waste C'( +38 h .t\CS.seek 0ml C''4 +506 h '4 C'v& h &W C'O% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +507 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'&'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'.& h &W C'@% h %4 .t\CSV.dial CSR C'C  +150 h  C'"% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'm'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'U' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'i' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'?  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'1  +113 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CTV C'9  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C') @ +38 h @ .t\CS.seek 0ml C'S'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'b  +37 h  C'6% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  +37 h  .t\CBV.dial :waste2b C'j  +76 h  .t\CS.seek 0ml C'3'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'|  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P '*  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'r'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'% +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'& h &W C'I% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'g% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'= .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'D'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +506 h '0 .t\CSV.dial :waste C'o +38 h .t\CS.seek 0ml C'{'4 +506 h '4 C'a& h &W C'T% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'7% h %0 .t\CS.seek 5ml C']'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'w +75 h .t\CS.seek 0ml C'G'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'8& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'k% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '}  +37 h  +113  .t\CBV.dial :waste1 C'x @ +38 h @ .t\CS.seek 0ml C'd'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'q& h &W C'z% h %4 .t\CSV.dial :flush C'2  +75 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +38 h @ C'u% h %p .t\CS.seek 7ml C'>'i +416 h 'i ."connect"\CSV.dial CTV C'M  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C';& h &W C'% h %4 .t\CSV.dial CSR C'K  +37 h  C'e% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'y'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'-  'N +38 h +75 .t\CTV.dial :intake C'p  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'J' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '^'4 +607 h '4 .t\CSV.dial CSR C't  +113 h  C'/% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'L'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'<' +1372 h ' .t\CSV.dial CBV C'_ +113 h .t\CS.seek 0ml C'E'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +6 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.seek CC M&\ ' +91 h ' .tHand.close M&(/ +150 h / .t\CC.seek :open C'5( +416 h ( M&v& h &ӷ M&O% h % .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&'1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +90 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&.1 +2 h 1 M&@& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &C(- +7 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &- .t\Forearm.seek :garage M&m'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&U% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &#' +91 h ' .t\CC.seek :closed C',(W +416 h (W C'9& h &W .tHand.open M&1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'b& h &W C'6% h %4 .t\CSV.dial CSR C'S  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'n'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'| +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'3& h &W C'% h %4 .t\CSV.dial :flush C'*  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'r'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'%  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'I  +75 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'g'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'='4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'D +38 h .t\CS.seek 0ml C'o'4 +506 h '4 C'0& h &W C'a% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.seek 3ml C'['0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +37 h @ C'h% h %0 .t\CS.seek 5ml C'7'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'w'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +7 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&G' +90 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'k( +416 h ( M& & h &ӷ M&% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +265 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&21 +150 h 1 M&d% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #t\Completed [mbaPhase] begun 05:06:10 #\Extending 18tundeploy until completion of MFBPCR +235183 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct3108h25ml.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*M  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*K% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*-'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*t% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b */'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +31 h  ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*'% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct3110h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*m  +42 h  ."MFBPCR"\ARV.dial :carrier F*1  +11 h  F*s% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*)  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*j  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*|' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*%'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*I' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct3110h25ml.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *T'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*l  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +32 h  ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*z% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*M'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *K'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct3112h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*/  +10 h  F*^% h %V ."MFBPCR"\AS.seek 0.5ml F*L'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*v I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*+% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*'  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*:% h %< ."MFBPCR"\AS.seek 0.594ml F*.'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*@ n +21 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*&'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*m% h % \\ ."MFBPCR"\AS.seek 0.326ml F*1'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct3112h25ml.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*?  +22 h ."MFBPCR"\ATV.dial :air3 F*9 % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *b'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +21 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +21 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*|  +65 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*%  +31 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*g'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*0% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct3115h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*!  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *f'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*} n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*u I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*Z% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*; n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*p' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*-  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*J% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*^ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct3115h25ml.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*<  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +21 h F*c% h %Z ."MFBPCR"\AS.seek 0.81ml F*~'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*'  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*X  +65 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*s'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*1'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*9% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*5  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt218oct3117h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*%  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*I n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*Y  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*g' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +22 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*{ n +21 h n ."MFBPCR"delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F*!'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*i % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt218oct3117h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*, % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*>  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*N  +32 h  ."MFBPCR"\ATV.dial :air3 F*y % +86 h % F*-% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*L  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *V'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct3119h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*:'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*\\ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*s' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*?  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F*1'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +22 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*6'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*S  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*E  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*3% h % \\ ."MFBPCR"\AS.seek 0.326ml F**'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct3119h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*| @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *%' +424 h ' ."MFBPCR"\ATV.dial :ASV F*r n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*I'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *Y'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*='(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *0' +423 h ' ."MFBPCR"\ATV.dial :air3 F*g% h % ."MFBPCR"\AS.seek 0.53ml F*D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*R% h % ."MFBPCR"\AS.seek 0.71ml F*{' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h % H ."MFBPCR"\AS.seek 0.735ml F*7' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*[% h % Ű ."MFBPCR"\AS.seek 0.72ml F*]' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*l% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*!% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*H' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %  ."MFBPCR"\AS.seek 0.604ml F*q'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*z% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*G' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*u n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Q  +22 h  ."MFBPCR"\AS.seek 0ml F*f'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *>'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*d% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*M% h % ."MFBPCR"\AS.seek 0.58ml F*;' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h % q ."MFBPCR"\AS.seek 0.605ml F*N'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*-% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*e' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*J n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*p  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*/  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *<' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*L n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +202 h 'R ."MFBPCR"\SPE.coast F*V  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*c'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*( n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*v  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *~' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*'  +43 h  F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*X% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *:'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*@ n +65 h n F*A% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*.  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*\\% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *C'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*?% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *9' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*)  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *1': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *b'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*6 n +151 h n ."MFBPCR"\AS.seek 0ml F*S'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*# % +43 h % F*E% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *j' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +21 h F*n% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F**' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*P'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*r I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*Y  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *=' +9988 h ' ."MFBPCR"\ATV.dial AIV F*g I +43 h I ."MFBPCR"\AS.seek 0.02ml F*0' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +129 h n ."MFBPCR"\AS.seek 0ml F*R'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*D% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +32 h  ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*d% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*K% h %m ."MFBPCR"\AS.seek 0.82ml F*;'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *<'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*('V +2547 h 'V #t\Asleep after completing 18tundeploy assays .GoodNews.suppressMail "{1 => 22, 2 => 22, 3 => 22, 4 => 9, 5 => 13, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:14:44CEST30-Aug-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tundeploy assays" #\Mission runtime was 1:01:52.400114 !!o:SignalException :\ signoi:bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" SIGHUP:@timestampu: Time >L) !o:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error #m\EXIT @1535656517.83CEST @1536242123.37CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:55:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:55:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:55:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 4 at 15:55:55CEST06-Sep-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:55:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F*~% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +222 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M& & h &- M&\ % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b & (w +166 h (w M& & h &w A( & h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(' +56 h ' A(& h &,9 M&& h &w .t\Elbow.seek 4 M&(w +7 h (w M&& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h &w M&% h %7 .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&"(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % C'/'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +1246 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C'99 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A(9'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ +2 A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +18 h ` .t\AIV.dial :bypass C'O { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&[% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +150 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +37 h  .t\PS.seek 0ml P%h' +1598 h ' .t\PSV.dial PSR P%^ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%k' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'm  P%j  h +76 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2609 h ' .t\PSV.dial :air P%;  +38 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +37 h .t\PS.seek 0ml P%t' +2610 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2609 h ' .t\PSV.dial :mixloop P%? +38 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +6 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C' & h & M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &,9 M& & h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A( & h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &,9 M&#& h &w .t\Elbow.seek 4 M&(w +6 h (w M&& h &w A((& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&s& h &w M&,% h %7 .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +143 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%)  +37 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2609 h ' .t\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +37 h @ .t\CS.seek 10ml C'I' +2648 h ' .t\CSV.dial CTV C'H  +37 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +151 h C'l& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &L( +143 h ( M&P& h & A(9& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +264 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &[' +26 h ' .t\Elbow.hold M&K, +6 h , .t\Elbow.reconfigure TightElbow A(V- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(Y' +27 h ' M&\\% h % A(Q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &U'B +20 h 'B M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +37 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +90 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &C( +143 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &t' +92 h ' .t\Elbow.hold M&?, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h % A(q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&{'B +21 h 'B M&~& h & A(Z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%z  + A(6 +112 h  P%y% h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%$ +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +18 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +251 h ' .t\PRV.dial :waste2 P%} @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M&\ / +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%)  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +76 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%A' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +75 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%l  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +75 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +7 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %9'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& F*& @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +21 h  ."MFBPCR"delay 20 seconds +92 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *['6 +66 .t\PSV.dial :waste1 P%g  +38 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%k' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +37 h  .t\PS.seek 0ml P%h' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +21 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%q +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%~' +216 ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +38 h  .t\PS.seek 0ml P%z' +739 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%$' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P% +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +850 h ' .t\PSV.dial :air P%\  +112 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%(  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +619 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F**% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C')'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +37 h  +113  .t\CBV.dial :waste1 C'! @ +38 h @ .t\CS.seek 0ml C':'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +22 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +1626 h ' ."connect"\CSV.dial CTV C'G  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'9( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +264 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&@1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&^'η +90 h 'η .tHand.close M&_/ +150 h / M&Y& h &- M&j% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +265 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C';(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +30 h (W C'e& h &W .tHand.open M&x1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'Z% h %4 .t\CSV.dial CSR C']  +37 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'i'p +244 h 'R ."MFBPCR"\ATV.dial :air2 F*C  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *~' +1106 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'y% h %4 .t\CSV.dial :flush C'c  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'8% h %p .t\CS.seek 7ml C'z'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +37 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\  +38 h  .t\CBV.dial :waste1 C'# @ +75 h @ .t\CS.seek 0ml C's'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'm'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +37 h +76 .t\CTV.dial :air C'5  +150 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +562 h ' ."MFBPCR"delay 5 seconds +138 .t\CS.seek 3ml C''0 +362 ."MFBPCR"\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F**' +79 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'.% h %4 .t\CSV.dial :flush C')  +37 h  .t\CS.seek 5ml C'7'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'!% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'2 +37 h .t\CS.seek 0ml C'J'4 +507 h '4 C':& h &W C'<% h %4 .t\CSV.dial :flush C'>  +37 h  .t\CS.seek 3ml C'E'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'D% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +506 h '0 .t\CSV.dial :waste C'l +76 h .t\CS.seek 0ml C'R'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'S& h &W C'=% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  '%  +38 h  +112  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C'\\'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'Q& h &W C'N% h %4 .t\CSV.dial :flush C'a  +75 h  .t\CS.seek 5ml C'f'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +37 h @ C'L% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'g  +38 h  .t\CBV.dial :waste2b C'1  +75 h  .t\CS.seek 0ml C'U'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +7 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&p& h &ӷ M&[% h % .t\Forearm.seek :retract M& '\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +265 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&x1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&I1 +2 h 1 M&]& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +6 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&u/ +150 h / M&v& h &- M&i% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +265 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +90 h ' .t\CC.seek :closed C'b(W +416 h (W C'r& h &W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'z& h &W C' % h %4 .t\CSV.dial CSR C'  +38 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '$'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '}'[ +463 h '[ .t\CSV.dial :waste C'w +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +506 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F*% h % ."MFBPCR"\AS.seek 0.52ml F*' +939 h '4 #t\Purging external Intake C' & h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\ ' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'# .t\CBV.dial CSV C'  +38 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'('4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C's  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'5 +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C'~'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'0% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C')'4 +651 h '4 .tdelay 7 seconds +85 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteR F*/  +21 h  ."MFBPCR"\AS.seek 0.3ml F*!': +30 .t\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'n'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&<' +90 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'J( +416 h ( M&& h &ӷ M&D% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +265 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&S1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [coreNEG] begun 01:00:31 #\Extending 18tun until completion of MFBPCR +1977 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*R'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *%'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*Q'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*a  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *\\% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *T' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *L% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *f' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*g% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *1' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*@% h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Y n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*V % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *o'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*; I +21 h I F*p% h %v ."MFBPCR"\AS.seek 0.059ml F*['H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*W % +21 h % F*9% h %H ."MFBPCR"\AS.seek 0.069ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h % ."MFBPCR"\AS.seek 0.769ml F*k' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h % q ."MFBPCR"\AS.seek 0.794ml F*I' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*C'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*e I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*y% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*$% h %V ."MFBPCR"\AS.seek 0.59ml F*w' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F* % h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*M % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.23ml F*s' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *5'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F* @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*+'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1032 h '\  F*.% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*/% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*)% h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*7% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *A' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3472 h 'V ."MFBPCR"\SPE.coast F*>  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +21 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*R  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*"' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +22 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 F*1  +65 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F*W % +86 h % F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +21 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +65 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0104h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*m  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*0  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +108 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0104h.pcr F*<% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*6  +21 h ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*!'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*%  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +21 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +21 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0107h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +10 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*^  +22 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*r% h %Fj ."MFBPCR"\AS.seek 0.524ml F*8' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*U  +107 h  ."MFBPCR"delay 7 seconds +700 F*$% h %< ."MFBPCR"\AS.seek 0.594ml F*}'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.6ml F**'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*M  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0107h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*(  +22 h ."MFBPCR"\ATV.dial :air3 F*s % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *''Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*<'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*S  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0109h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*N  +11 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*g  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +21 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*L n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*X' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*`  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*;' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*j  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.536ml F*W'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +22 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*9'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +22 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*K  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0109h.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*q  +21 h ."MFBPCR"\ATV.dial :air3 F*i % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*8  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*c  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*3 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*t I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*: % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +22 h n ."MFBPCR"delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.6ml F*G'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +87 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*`% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +65 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*Z % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +21 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *C'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0114h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*$ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*w  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*} I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*M % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*('< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*5' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0114h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*0  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*.  +150 h F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *)' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*F  +43 h  F*7% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*3 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *>'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +424 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*S% h % ."MFBPCR"\AS.seek 0.71ml F*6' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h % H ."MFBPCR"\AS.seek 0.735ml F*2' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*G' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*! n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7162 h ' ."MFBPCR"\ATV.dial :waste F*R n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*B% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*Q% h % ."MFBPCR"\AS.seek 0.579ml F*\\' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %  ."MFBPCR"\AS.seek 0.604ml F*@'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*T% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*L' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*1 n +85 h n ."MFBPCR"\ASV.dial :wasteR F*Y  +22 h  ."MFBPCR"\AS.seek 0ml F*`'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *X'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*j % +107 h % F*o% h %V ."MFBPCR"\AS.seek 0.01ml F*&' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*V% h % ."MFBPCR"\AS.seek 0.58ml F*p' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h % q ."MFBPCR"\AS.seek 0.605ml F*x'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Z% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*[' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*9 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*K  +21 h  ."MFBPCR"\AS.seek 0ml F*k'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*I  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *d' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *q'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*4 n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +202 h 'R ."MFBPCR"\SPE.coast F*i  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*^  +43 h  F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *y' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *c': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*z% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *8'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*$% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *U' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *}': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F**% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *M'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*# n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*\ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*( n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*, n +151 h n ."MFBPCR"\AS.seek 0ml F*s'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*'  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*m % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*+% h % ."MFBPCR"\AS.seek 0.96ml F*5'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*0' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*.% h % ."MFBPCR"\AS.seek 0.97ml F*/'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*~ I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *F'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *)'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*>% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *A' +9988 h ' ."MFBPCR"\ATV.dial AIV F*t I +43 h I ."MFBPCR"\AS.seek 0.02ml F*b' +9880 h ' ."MFBPCR"\ATV.dial :waste F*: n +128 h n ."MFBPCR"\AS.seek 0ml F*<'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*S% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*D  +22 h ."MFBPCR"\ATV.dial :air3 F*l % +43 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*!'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +21 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +21 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *i'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*q % +107 h % ."MFBPCR"\AS.seek 0ml F*u'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 2, 4 => 20, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:55:59, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 2:34.25472 #-> exit #m\EXIT @1536242687.49CEST @1536341786.85CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:36:36, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:36:55, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& h & S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:37:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 19:37:06CEST07-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:37:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'& ."readyMFB"\AFV.select :holdingCoil F* h & M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&% h %8 .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'I% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C' 9 h 9Td +100 C'I9 h 9Xd +100 C'E9 h 9(\\d +100 C'F9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'H9 h 9xd +100 C'R9 h 9|d +100 C'S9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'Q9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 (( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b M&I& A( & h &ӷ &( .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(E'Z .t\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(H& h &( A(R& h &( M&S& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&W& h & A(Q& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'C +92 h 'C .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %C A(]& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&\\'o +21 h 'o M&^& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ + A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C'e { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'  P%\  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A()' +66 h ' A(9& h &  M&8& h & .t\Elbow.seek 2 M&=( +7 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down A(&'X M&A& h & M&B% h %8 .t\Forearm.seek :retract M&D'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&:(ӷ +142 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'S'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%Q +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +150 h  .t\PS.seek :empty P%Y' +2610 h ' .t\CBV.dial :waste1 C'] @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +38 h @ .t\CS.seek 10ml C'c' +2647 h ' .t\CSV.dial CTV C'Z  +38 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +150 h C'E& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h &( M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +142 h ( M&o& h & A(b& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(@& h &( .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +265 h '\\ M&r& h & A(C& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'C +25 h 'C .t\Elbow.hold M&a, +7 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %C A(p& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&u'o +21 h 'o M&O& h & A(t& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C' ( +416 h ( A(& h &( A( & h &( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +142 h ( M& & h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +21 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%,' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%2  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&9(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&A% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'.(W +415 h (W C')& h &W .tHand.open M&&1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%H' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Q'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%[' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +76 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +75 h @ .t\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%E  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +76 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +6 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*O @@T .t\AWV.select :air F*t  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%2' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%'  +113 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*)% h %Q ."MFBPCR"\AS.seek 0.01ml F*&' +171 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*I'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  'M  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'Q'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +109 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*S n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'E' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&:% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M&\ & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A( 'X M&& h & M&x% h %8 ."WCR"\Forearm.seek :retract M&{'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*$  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *2'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*('(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*8% h %(A ."MFBPCR"\AS.seek 0.6ml F*A'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*G% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *9' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +3473 h 'V ."MFBPCR"\SPE.coast F*<  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*)  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +95 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'D ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'q  ':  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'6' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '\  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +75 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &$' +89 h ' A(z& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +254 h 'K M&/& h & A(2& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'C +25 h 'C ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&4% h %C A((& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&0'o +21 h 'o M&3& h & A(8& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A( ' +36 h ' M&A& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ ."WCR"\Forearm.seek CC M&)' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'*( +416 h ( A(1& h &( A(& h &( M&&& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A( & h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&B% h %C A(U& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&H'o +21 h 'o M&%& h & A(F& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Q& h & .tHand.open M&]1 + A(6 + h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&S& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'q% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'6& h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'}  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'? @ +75 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'c  +38 h  C'{% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +709 h ' ."connect"\CSV.dial CTV C'v  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'Y'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'/  +151 h .t\CS.seek 0ml C'2'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'4 +37 h .t\CS.seek 0ml C'('4 +507 h '4 #t\Flushing upper manifold C'3& h &W C'8% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'>% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'0 +38 h .t\CS.seek 0ml C''4 +506 h '4 C''& h &W C',% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'1% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'&'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C' '4 +507 h '4 #t\Flushing lower manifold's waste1 port C')& h &W C'% h %4 .t\CSV.dial :flush C'R  +37 h  .t\CS.seek 5ml C'B'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +37 h @ C'%% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  'X  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'L'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'M& h &W C'Q% h %4 .t\CSV.dial :flush C']  +76 h  .t\CS.seek 5ml C'_'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'% h %p .t\CS.seek 7ml C'H'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'^'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&Z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&S/ +150 h / .t\CC.seek :open C'P( +416 h ( M&W& h &ӷ M&\\% h % .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &e(- +264 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&.1 +150 h 1 M&"% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&q1 +2 h 1 M&h& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&C'η +91 h 'η .tHand.close M&d/ +150 h / M&T& h &- M&% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'|(W +416 h (W C'n& h &W .tHand.open M&\ 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +37 h  C'j% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'y'4 +2009 h '4 #t\Purging external Intake C'x& h &W C';% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w '  +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'?'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'c& h &W C'{% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +37 h @ C'% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'/% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'2'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'7% h %0 .t\CS.seek 5ml C'3'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'8'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +507 h '0 .t\CSV.dial :waste C'( +75 h .t\CS.seek 0ml C'$'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&>& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&9' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'<( +416 h ( M&'& h &ӷ M&,% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +256961 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*J% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*B% h %Z ."MFBPCR"\AS.seek 0.81ml F*N'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*F'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*l  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*f'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*:  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*6'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*a  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*t  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*r' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*x  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*}'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*y  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*c  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Y  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*8'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*A% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +64 h  F*0% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *`'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*G % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*V  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +21 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*\\ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*m n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.53ml F*.' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*"  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*:'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +108 h  ."MFBPCR"delay 7 seconds +700 F*q% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +22 h n ."MFBPCR"delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.6ml F**'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*e  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*D  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*P  +21 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*x'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*y% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*0  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +10 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *,'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*G  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*= n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*&' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*J I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*R % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +22 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*N  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*F  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*L  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F*]'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*+% h %Z ."MFBPCR"\AS.seek 0.81ml F*S'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*f% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*.% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*T  +31 h  ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +21 h F*\ % h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*n  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ARV.dial :carrier F*x  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *{'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*s % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*~' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*4 n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.65ml F*(' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*0'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*` % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*! @@T *K  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*|  +150 h F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *1' +424 h ' ."MFBPCR"\ATV.dial :ASV F*G n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*9% h % ."MFBPCR"\AS.seek 0.62ml F*='\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *J'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*E'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*& % +43 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*U' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h % H ."MFBPCR"\AS.seek 0.735ml F*N' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F* % h % Ű ."MFBPCR"\AS.seek 0.72ml F*F' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*L n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*l% h % zG ."MFBPCR"\AS.seek 0.365ml F*Q' +7162 h ' ."MFBPCR"\ATV.dial :waste F*_ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +21 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %  ."MFBPCR"\AS.seek 0.604ml F*V'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F*o% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*S' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*m n +85 h n ."MFBPCR"\ASV.dial :wasteR F*f  +22 h  ."MFBPCR"\AS.seek 0ml F*W'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *['V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*. % +107 h % F*g% h %V ."MFBPCR"\AS.seek 0.01ml F*i' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*:% h % ."MFBPCR"\AS.seek 0.58ml F*5' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h % q ."MFBPCR"\AS.seek 0.605ml F*T'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*e% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*d' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*D'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *6'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *P' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *p'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*n n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*a n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*^  +43 h  F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *t' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*;  +43 h  F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *x': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *}'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *w' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*c  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*{  +65 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*Y% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*~ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*/  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*4 n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *z'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*3'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*7  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*2 % +43 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*(% h % ."MFBPCR"\AS.seek 0.96ml F*>'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +21 h % ."MFBPCR"delay 3 seconds +300 F*K% h %0 ."MFBPCR"\AS.seek 0.985ml F*0' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*,% h % ."MFBPCR"\AS.seek 0.97ml F*''c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*G I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *9'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*J  +43 h  F*&% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *E' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F*) n +128 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*%% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*B  +22 h ."MFBPCR"\ATV.dial :air3 F*U % +43 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*L% h %Z ."MFBPCR"\AS.seek 0.81ml F*l'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +21 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*H  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *@'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*f  +32 h  ."MFBPCR"\ATV.dial :air3 F*S % +86 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*['> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +21 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F**  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *P'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *p'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*u % +107 h % ."MFBPCR"\AS.seek 0ml F*a'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@19:38:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:39.4684 #-> exit #m\EXIT @1536342261.62CEST @1536342270.83CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:46:29CET01-Jan-70 .delayUntil "16:46:29.00" +5678899 #-> exit #m\EXIT @1536342283.22CEST @1536506090.55CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 17:15:18CEST09-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml" F*/% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'0& ."readyMFB"\AFV.select :holdingCoil F* h & .tHand.open M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h & M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'+9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'$9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'4 +113 h .t\CBV.dial CSV C'a  +18 h C'`% h %4 .t\CS.seek 5ml C'c'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +37 h C'e% h %p .t\CS.seek 7ml C']'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +37 h @ .t\CS.seek 10ml C'h' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'i ` +132 h ` #tLysing Complete C'j& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(f% h %X A(n. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (o(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (p& M&q& h &( &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(l'Z .t\Elbow.seek CC M&$&.,(\ #b &m(ӷ +7 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(r& h &( A(y& h &( M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +23 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +150 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%8' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C':  P%9  h +76 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%=  +113 h .t\PS.seek 0ml P%?' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2609 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +6 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&R% h %\  A($% h %X A(X. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (T( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +165 h ( M&W& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(4& h &  .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(`& h &  M&_& h & .t\Elbow.seek 2 M&d( +6 h ( M&e& h & A(c& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down M&]& A(i'X h & M&j% h %8 .t\Forearm.seek :retract M&k'\ +65 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&f(ӷ +143 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'r'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%m +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C' ' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'l& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &o' +89 h ' A(h& h &( .t\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +26 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&*% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&('o +20 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +37 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C':( +415 h ( A(0& h &( A(=& h &( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&<& h & A(E& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &D'C +92 h 'C .t\Elbow.hold M&B, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h %C A(&& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&J'o +20 h 'o M&>& h & A(+& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  + A(6 +112 h  P%G% h % .t\PS.seek 0.499ml P%Q' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +38 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +37 h .t\CBV.dial AIV C'R  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%X  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&^1 +2 h 1 M&4& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&`(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&Y& h &- M&]% h %η .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&i1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%q' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%r' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +76 h .tdelay 2 seconds +200 P%m% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P% ' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%l  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'h { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %f' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '*& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*w @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +21 h  ."MFBPCR"delay 20 seconds +92 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%o' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +38 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%8' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +37 h  .t\PS.seek 0ml P%6' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +42 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*?' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%K' +739 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%.' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +112 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %4'k +2679 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%Y' +2609 h ' .t\PSV.dial :mixloop P%] +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5106 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*n  +43 h  F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *j'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*i' +171 h ' .t\PSV.dial :mixloop P%d +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*p'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*q% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'H' +1626 h ' ."connect"\CSV.dial CTV C'r  .t\CTV.dial CSV C'u  +37 h  +113  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C'{'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&f% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&1& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &b(- +7 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&9% h %η .t\Forearm.seek :retract M&s'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +265 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*0 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +30 h (W C'5& h &W .tHand.open M&)1 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'&& h &W C'+% h %4 .t\CSV.dial CSR C'  +37 h  C'6% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'7'p +244 h 'R ."MFBPCR"\ATV.dial :air2 F*A  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *>' +1106 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ';% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'D'[ +463 h '[ .t\CSV.dial :waste C'M +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'J& h &W C'G% h %4 .t\CSV.dial :flush C'/  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'N'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +38 h @ C'% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'F  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'.'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'R& h &W C'$% h %4 .t\CSV.dial :flush C'V  +76 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'[% h %p .t\CS.seek 7ml C'Q'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'^  +76 h  .t\CS.seek 0ml C'O'4 +765 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'@  +38 h  C'4% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  +38 h  .t\CBV.dial :waste1 C'_ @ +75 h @ .t\CS.seek 0ml C']'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'Y  +38 h  C'c% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'I'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  +38 h  .t\CBV.dial :waste2b C'B  +75 h  .t\CS.seek 0ml C':'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'a  +37 h  C'd% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'j'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C't 'i  +37 h +76 .t\CTV.dial :air C'v  +150 h .t\CS.seek 0ml C'S'4 +910 h '4 .tdelay 7 seconds +562 h ' ."MFBPCR"delay 5 seconds +138 .t\CS.seek 3ml C'p'0 +362 ."MFBPCR"\ATV.dial ASV F*C n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*q' +79 h '0 .t\CSV.dial :waste C'P +38 h .t\CS.seek 0ml C'T'4 +507 h '4 #t\Flushing upper manifold C'y& h &W C'% h %4 .t\CSV.dial :flush C'}  +37 h  .t\CS.seek 5ml C'r'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +37 h @ C'm% h %p .t\CS.seek 7ml C'H'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'{& h &W C' % h %4 .t\CSV.dial :flush C'\  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'|'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'l +75 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'~% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\\ @ +38 h @ C'k% h %p .t\CS.seek 7ml C'g'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'L  'f  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'*'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C' % h %4 .t\CSV.dial :flush C'2  +75 h  .t\CS.seek 5ml C'3'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'1  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'o  +75 h  .t\CS.seek 0ml C'('4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'%( +416 h ( M&=& h &ӷ M&"% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &s(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&)1 +150 h 1 M&8% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M& 1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &6(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&E/ +150 h / M&;& h &- M&7% h %η .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +265 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' .t\CC.seek :closed C''(W +415 h (W C'?& h &W .tHand.open M&1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'K& h &W C'R% h %4 .t\CSV.dial CSR C'$  +38 h  C'V% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '.'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'X% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '['[ +463 h '[ .t\CSV.dial :waste C'! +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +506 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*^  +65 h  F*Q% h % ."MFBPCR"\AS.seek 0.52ml F*@' +939 h '4 #t\Flushing lower manifold's waste1 port C'4& h &W C'O% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'W% h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CTV C'c  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 C'] @ +76 h @ .t\CS.seek 0ml C'I'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'`& h &W C'B% h %4 .t\CSV.dial :flush C'n  +76 h  .t\CS.seek 5ml C'a'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +38 h @ C':% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CTV C'i  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C't  +76 h  .t\CS.seek 0ml C'v'4 +766 h '4 #tDisinfecting external Intake with kill C'Z& h &W C'S% h %4 .t\CSV.dial CSR C'C  +37 h  C'P% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'>'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'p' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'y '  +37 h +76 .t\CTV.dial :intake C'}  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'T' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'u  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'm'4 +606 h '4 .t\CSV.dial CSR C'r  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'H'p +459 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0.3ml F*x': +327 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'{' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'\ '4 +2127 h ': ."MFBPCR"delay 20 seconds +521 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &z(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'l( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&h1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +129 ."MFBPCR"\AS.seek 0.07ml F*'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*g % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b **'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +43 h I F* % h %N ."MFBPCR"\AS.seek 0.109ml F*3'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*1 n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*o  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *9' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *,' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*<% h % ."MFBPCR"\AS.seek 0.279ml F*'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*= n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *0'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F*)% h %v ."MFBPCR"\AS.seek 0.059ml F*8'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*b % +21 h % F*+% h %H ."MFBPCR"\AS.seek 0.069ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F*A' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h % q ."MFBPCR"\AS.seek 0.794ml F*;' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*s% h % k ."MFBPCR"\AS.seek 0.779ml F*&'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *J' +4416 h ' ."MFBPCR"\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.seek :empty F*6'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*M % +43 h % F*%% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*N% h % ."MFBPCR"\AS.seek 0.579ml F*K' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F*$% h %  ."MFBPCR"\AS.seek 0.604ml F*V'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +21 h F*(% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :wasteR F*!  +43 h  ."MFBPCR"\AS.seek 0ml F*['V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*q % +108 h % F*4% h %V ."MFBPCR"\AS.seek 0.59ml F*O' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*_' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*W  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*U I +86 h I F*c% h % ."MFBPCR"\AS.seek 0.12ml F*]' +625 h ' ."MFBPCR"\ATV.dial :air3 F*Y % +21 h % F*`% h % ."MFBPCR"\AS.seek 0.13ml F*B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*I% h % ."MFBPCR"\AS.seek 0.23ml F*d' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h % ."MFBPCR"\AS.seek 0.255ml F*i'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*j% h %X ."MFBPCR"\AS.seek 0.24ml F*Z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*C  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *>'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*} I +85 h I ."MFBPCR"\AS.seek 0ml F*p'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*u'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*T  +42 h  #"MFBPCR"\Swish #1 of 2 F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*m% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*@'\  +1031 h '\  F*H% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*{% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*|  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*D  +64 h  ."MFBPCR"\ARV.dial :carrier F*F  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +21 h ."MFBPCR"delay 2 seconds +200 F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *~'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*k  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*2' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +107 h  ."MFBPCR"delay 7 seconds +700 F*1% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*9' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*<  +43 h  ."MFBPCR"\ASV.dial :waste1 F*,  +65 h ."MFBPCR"\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*0  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*s% h %n ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*&  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *M'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*$ % +87 h % F*K% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*.'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ARV.dial :carrier F*B  +11 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*a'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*t n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*P  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*>% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*y  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*T' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*r  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*m'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +22 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*D  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*''{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *L'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*x'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F**  +65 h  F*1% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*<  +32 h  ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*+'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*)  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*;  +10 h  F*M% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*R  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +22 h ."MFBPCR"delay 2 seconds +200 F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*( I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*/' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*! n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*.' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*^  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Q  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*U  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*_' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*t  +22 h ."MFBPCR"\ATV.dial :air3 F*d % +86 h % F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*Z% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*r  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*{ % +87 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ARV.dial :carrier F*1  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +22 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *='Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*" % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.536ml F*b'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*8'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*5  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*7  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*s  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*A% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*; % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*%  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +86 h % F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*$% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*V'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*X'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*^  +64 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *.'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*U  +32 h  ."MFBPCR"\ATV.dial :air3 F*c % +86 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +21 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*n'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ARV.dial :carrier F*T  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *|'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*F% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*D % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*L  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*x  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*g  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *2  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*3  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *o' +423 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*<  +43 h  F*,% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *='V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*w % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *"' +424 h ' ."MFBPCR"\ATV.dial :air3 F*0% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*)% h % ."MFBPCR"\AS.seek 0.71ml F*b' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*+' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +21 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*8' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*7 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*s% h % zG ."MFBPCR"\AS.seek 0.365ml F*A' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*; % +43 h % F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*6% h % ."MFBPCR"\AS.seek 0.579ml F*J' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %  ."MFBPCR"\AS.seek 0.604ml F*$'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*N% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*(' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +86 h n ."MFBPCR"\ASV.dial :wasteR F*?  +22 h  ."MFBPCR"\AS.seek 0ml F*!'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*^ % +108 h % F*q% h %V ."MFBPCR"\AS.seek 0.01ml F*X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.58ml F*4' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*O'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +21 h F*c% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*W' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*e n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*`  +21 h  ."MFBPCR"\AS.seek 0ml F*]'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *B'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*:  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *_' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*I n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *t'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*n n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +202 h 'R ."MFBPCR"\SPE.coast F*d  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*a'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*C n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*Z  +43 h  F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *P' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*> I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*p  +42 h  F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *u': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*T  +64 h  F*}% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *r'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*m% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*H I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*{  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*F  +64 h  F*|% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *D'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *h': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*\\ n +150 h n ."MFBPCR"\AS.seek 0ml F*z'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*L  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*~ % +43 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %0 ."MFBPCR"\AS.seek 0.985ml F*2' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*3% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *<'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*9  +43 h  F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *w' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b * ' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*0' +9880 h ' ."MFBPCR"\ATV.dial :waste F*" n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*)% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *5'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*+% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*%  +64 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*(% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +21 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*[% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*4  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *d'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*t % +108 h % ."MFBPCR"\AS.seek 0ml F*j'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 2, 2 => 20, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:21.77928 #-> exit #m\EXIT @1536507631.89CEST @1536751444.39CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ P% 9\ % 2\ C' 0\ P% *\ S)&\ F*\ 9\ C' -\ P% 0\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 13:24:53CEST12-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:24:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*/% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Homing MFB Syringe C'0& ."readyMFB"\AFV.select :holdingCoil F* h & .tHand.open M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down A(I'X M&H& h & M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C',9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C'+9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'-9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'$9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'4 +113 h .t\CBV.dial CSV C'a  +18 h C'`% h %4 .t\CS.seek 5ml C'c'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +37 h C'e% h %p .t\CS.seek 7ml C']'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +37 h @ .t\CS.seek 10ml C'h' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'i ` +132 h ` #tLysing Complete C'j& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(f% h %X A(n. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (o(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (p& M&q& h &ӷ &( .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(l'Z .t\Elbow.seek CC M&$&.,(\ #b &m(ӷ +7 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(r& h &( A(y& h &( M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +23 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +150 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%8' +2609 h ' ."connect"\PRV.dial CBV P%9  .t\CBV.dial PRV C':  h +76 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%=  +113 h .t\PS.seek 0ml P%?' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2609 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +6 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&R% h %\  A($% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (T( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +165 h ( M&W& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(4& h &  .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(`& h &  M&_& h & .t\Elbow.seek 2 M&d( +6 h ( M&e& h & A(c& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &X/ +150 h / ."downElevator"\SE.seek :down M&i& A(]'X h & M&j% h %8 .t\Forearm.seek :retract M&k'\ +65 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&f(ӷ +143 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'r'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%m +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C' ' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'l& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &o' +89 h ' A(h& h &( .t\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +26 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&*% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&('o +20 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +37 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C':( +415 h ( A(0& h &( A(=& h &( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&<& h & A(E& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &D'C +92 h 'C .t\Elbow.hold M&B, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h %C A(&& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&J'o +20 h 'o M&>& h & A(,& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  + A(6 +112 h  P%G% h % .t\PS.seek 0.499ml P%Q' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%- +38 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +37 h .t\CBV.dial AIV C'R  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%Y  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&^1 +2 h 1 M&4& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&`(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&X& h &- M&i% h %η .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&]1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%r' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +76 h .tdelay 2 seconds +200 P%m% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P% ' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%l  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'h { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %f' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '*& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*w @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +21 h  ."MFBPCR"delay 20 seconds +92 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%o' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +38 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%+ +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%8' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +37 h  .t\PS.seek 0ml P%6' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F*, I +42 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*?' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%K' +739 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%-' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +112 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %4'k +2679 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%X' +2609 h ' .t\PSV.dial :mixloop P%i +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5106 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*n  +43 h  F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *j'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +171 h ' .t\PSV.dial :mixloop P%d +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*q'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*p% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'H' +1626 h ' ."connect"\CSV.dial CTV C'r  .t\CTV.dial CSV C'u  +37 h  +113  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C'{'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&f% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(b. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (o( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &  M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +90 h ' A(=& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&0( +6 h ( M&E& h & A(5& h &  ."WCR"\Forearm.seek 2 + 300 counts M&)'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &8/ +150 h / ."downElevator"\SE.seek :down A(<'X M&&& h & M&,% h %8 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +143 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)?+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*o% h %X ."MFBPCR"\AS.seek 0.24ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*R  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *Y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*- @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*^'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*@  +42 h  #"MFBPCR"\Swish #1 of 2 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *e'\  +1032 h '\  F*W% h %\  ."MFBPCR"\AS.seek 0.5ml F*U'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*_% h %(A ."MFBPCR"\AS.seek 0.6ml F*i'\  +1031 h '\  F*4% h %\  ."MFBPCR"\AS.seek 0.5ml F*I'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*n% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *`' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*B % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +3472 h 'V ."MFBPCR"\SPE.coast F*c  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*a  +64 h  ."MFBPCR"\ARV.dial :carrier F*d  +11 h  F*O% h %V ."MFBPCR"\AS.seek 0.5ml F*Z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +21 h ."MFBPCR"delay 2 seconds +200 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*P  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*}  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*m % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +254542 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'~  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'\\ @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'g  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'k ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'f  '*  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'2  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'3  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'b' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)1% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'% ` +57 h ` ."WCR"\CS.seek 7.288ml C's' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'=  '9  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'5  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'E% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'8' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)+% h % ."WCR"\CBV.dial between CSV and waste2b C',  +94 h  C'A% h % ."WCR"\CSV.dial between air and CTV C') ` +56 h ` ."WCR"\CS.seek 7.288ml C'7' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'D  '<  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'"  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&J% h %\  A(/% h %X A('. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (K(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h &( M&& h &ӷ M&M% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &o( +143 h ( M&>& h & A($& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +89 h ' A(?& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +253 h 'K M&V& h & A(Y& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +26 h 'C ."WCR"\Elbow.hold M&Q, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(^- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(@' +27 h ' M&[% h %C A(N& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&W'o +20 h 'o M&e& h & A(_& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &U' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(4' +36 h ' M&i& h & M&n% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  ."WCR"\Elbow.seek CC M&B(ӷ +143 h (ӷ M&`& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ ."WCR"\Forearm.seek CC M&a' +91 h ' ."WCR"Hand.close M&d/ +150 h / ."WCR"\CC.seek :open C'O( +416 h ( A(X& h &( A(Z& h &( M&]& h &ӷ M&t% h % ."WCR"\Forearm.seek :retract M&v'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &:( +143 h ( M&C& h & A(p& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &S'C +92 h 'C ."WCR"\Elbow.hold M&P, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &y1 +150 h 1 M&j% h %C A(}& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&r'o +20 h 'o M&T& h & A(m& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &H' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (x% h %B M&u% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&1 +2 A(6 h 1 M&\ & h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&z& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'g& h &W .tHand.open M&L1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'*& h &W C'f% h % .t\CSV.dial CSR C'  +38 h  C'2% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'b& h &W C' % h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'%% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  '9  +37 h  +113  .t\CBV.dial :waste1 C's @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'5% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'+  +37 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C'A'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C')  +38 h  C'&% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'D'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +709 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'" @ +75 h @ .t\CS.seek 0ml C'0'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV C''  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'F  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'M  +37 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '$'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6  'G +37 h +76 .t\CTV.dial :air C'V  +150 h .t\CS.seek 0ml C'Y'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +506 h '0 .t\CSV.dial :waste C'[ +38 h .t\CS.seek 0ml C'N'4 +507 h '4 #t\Flushing upper manifold C'e& h &W C'_% h %4 .t\CSV.dial :flush C'i  +37 h  .t\CS.seek 5ml C'n'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +37 h @ C'o% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'`'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +507 h '0 .t\CSV.dial :waste C'W +37 h .t\CS.seek 0ml C'@'4 +507 h '4 C'U& h &W C'Q% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.seek 3ml C'I'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'Z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C']'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'C +76 h .t\CS.seek 0ml C'p'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'B% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 5ml C'j'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'T% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  '  +37 h  +113  .t\CBV.dial :waste1 C': @ +38 h @ .t\CS.seek 0ml C'x'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'u& h &W C'{% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'\ 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'S% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  +38 h  .t\CBV.dial :waste2b C'H  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&g1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&f1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &2(- +6 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&3% h %η .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +265 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'%(W +416 h (W C'& h &W .tHand.open M&=1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'% h %4 .t\CSV.dial CSR C'5  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '8'[ +463 h '[ .t\CSV.dial :waste C'E +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '+'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C',& h &W C'% h %4 .t\CSV.dial :flush C')  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +38 h @ C'D% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 C'< @ +75 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'0% h %4 .t\CSV.dial :flush C'J  +76 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C'/% h %p .t\CS.seek 7ml C'M'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'>  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'$'4 +765 h '4 #tDisinfecting external Intake with kill C'6& h &W C'G% h %4 .t\CSV.dial CSR C'V  +38 h  C'?% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'Y'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'[' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'^ 'e  +37 h +76 .t\CTV.dial :intake C'_  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'i' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'N  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +606 h '4 .t\CSV.dial CSR C'o  +113 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +1372 h ' .t\CSV.dial CBV C'` +113 h .t\CS.seek 0ml C'W'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +6 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&;/ +150 h / .t\CC.seek :open C'X( +416 h ( M&I& h &ӷ M&Z% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &C(- +264 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&y1 +150 h 1 M&p% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +253112 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*T% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*4% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*r  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *P'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*9  +11 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*,  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*)' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*+  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*D' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*&  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.536ml F*<'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*7  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*J n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*F'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*>' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*6% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*$% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*e  +21 h ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +65 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*v'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*j'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *K'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +10 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*L I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*f n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*1  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*2' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*5  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*+'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*)% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*D  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*- % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*G'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *N'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ARV.dial :carrier F*c  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*a  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*] n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*T I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*v'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*u  +107 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*{'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*m n +21 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.65ml F*4' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*K% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*\\% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +21 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*<'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *''Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*> I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*6' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*- % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*?' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*e  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*G'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +22 h  ."MFBPCR"delay 7 seconds +700 F*_% h %< ."MFBPCR"\AS.seek 0.594ml F*N'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*R  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*`' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*;'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*d @@T ."MFBPCR"\ASV.dial :SPEdelivery F*s  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*%  +151 h F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *a' +423 h ' ."MFBPCR"\ATV.dial :ASV F*U n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*y  +43 h  F*@% h % ."MFBPCR"\AS.seek 0.62ml F*]'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*B n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *T'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*l'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*p % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*v% h % ."MFBPCR"\AS.seek 0.71ml F*m' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F* % h % Ű ."MFBPCR"\AS.seek 0.72ml F*S' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*r n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*4' +7161 h ' ."MFBPCR"\ATV.dial :waste F*P n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*~' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*f n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*g'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*\\ % +108 h % F**% h %V ."MFBPCR"\AS.seek 0.01ml F*O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*b% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*1' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*= n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2  +21 h  ."MFBPCR"\AS.seek 0ml F*|'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*(  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *5' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *9'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*E  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *,' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*"  +42 h  F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*D% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *<'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *0' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*F I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*'  +64 h  F*-% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *$'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*6 n +65 h n F*e% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *^' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*? I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*_  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *G': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*i n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *['Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*N n +150 h n ."MFBPCR"\AS.seek 0ml F*R'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*o  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*n % +43 h % F*!% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*`% h % ."MFBPCR"\AS.seek 0.96ml F*c'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %0 ."MFBPCR"\AS.seek 0.985ml F*;' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.97ml F*I'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*U I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *B'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *t'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*T  +43 h  F*p% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *l' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +43 h I ."MFBPCR"\AS.seek 0.02ml F*:' +9880 h ' ."MFBPCR"\ATV.dial :waste F*j n +129 h n ."MFBPCR"\AS.seek 0ml F*v'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*u% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*{  +21 h ."MFBPCR"\ATV.dial :air3 F*m % +43 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F**% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *9'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:26:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:44.321313 #-> exit #m\EXIT @1536754554.58CEST @1536754633.97CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi$; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:17:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:17:57, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:18:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1200ml .BACfans.suppressMail "Started mfb in tube 2 at 14:18:02CEST12-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:18:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1200ml" F*/% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'0& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil .tHand.open F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h & M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'*9 h 9 d +100 C'9 h 9 *d +100 C'+9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'$9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'4 +113 h .t\CBV.dial CSV C'a  +18 h C'`% h %4 .t\CS.seek 5ml C'c'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +37 h C'e% h %p .t\CS.seek 7ml C']'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +37 h @ .t\CS.seek 10ml C'h' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'i ` +132 h ` #tLysing Complete C'j& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&k% h %\  A(f% h %X A(n. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (o(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (p& h &( M&q& ."raiseStack"\SE.seek :upperLimit h &ӷ A(l'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +7 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(r& h &( A(y& h &( M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +23 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +150 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%8' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%9  C':  h +76 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%=  +113 h .t\PS.seek 0ml P%?' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2609 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +6 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&R% h %\  A($% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (T( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +165 h ( M&W& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(4& h &  .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(`& h &  M&_& h & .t\Elbow.seek 2 M&d( +6 h ( M&e& h & A(c& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &X/ +150 h / ."downElevator"\SE.seek :down A(]'X M&i& h & M&j% h %8 .t\Forearm.seek :retract M&k'\ +65 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&f(ӷ +143 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'r'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%m +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C' ' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'l& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &o' +89 h ' A(h& h &( .t\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +26 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&+% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&('o +20 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +37 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C':( +415 h ( A(0& h &( A(=& h &( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&<& h & A(E& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &D'C +92 h 'C .t\Elbow.hold M&B, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h %C A(&& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&J'o +20 h 'o M&>& h & A(*& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  + A(6 +112 h  P%G% h % .t\PS.seek 0.499ml P%Q' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +38 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +37 h .t\CBV.dial AIV C'R  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%Y  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&^1 +2 h 1 M&4& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&`(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&X& h &- M&i% h %η .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&]1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%q' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%r' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +76 h .tdelay 2 seconds +200 P%m% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P% ' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%l  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'h { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %f' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '+& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*w @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%2 +21 h  ."MFBPCR"delay 20 seconds +92 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%o' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +38 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%8' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +37 h  .t\PS.seek 0ml P%6' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F** I +42 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*?' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%K' +739 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%.' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +112 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %4'k +2679 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%X' +2609 h ' .t\PSV.dial :mixloop P%i +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5106 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*n  +43 h  F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *j'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*]' +171 h ' .t\PSV.dial :mixloop P%d +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*p'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*q% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'H' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'r  'u  +37 h  +113  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C'{'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&f% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1200ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(b. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (o( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &  M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +90 h ' A(=& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&0( +6 h ( M&E& h & A(5& h &  ."WCR"\Forearm.seek 2 + 300 counts M&)'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &8/ +150 h / ."downElevator"\SE.seek :down M&&& A(<'X h & M&*% h %8 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +143 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +30 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1200ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)?+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*o% h %X ."MFBPCR"\AS.seek 0.24ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*R  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *Y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*. @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*^'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*@  +42 h  #"MFBPCR"\Swish #1 of 2 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *e'\  +1032 h '\  F*W% h %\  ."MFBPCR"\AS.seek 0.5ml F*U'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*_% h %(A ."MFBPCR"\AS.seek 0.6ml F*i'\  +1031 h '\  F*4% h %\  ."MFBPCR"\AS.seek 0.5ml F*I'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*n% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *`' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*B % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +3472 h 'V ."MFBPCR"\SPE.coast F*c  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*a  +64 h  ."MFBPCR"\ARV.dial :carrier F*d  +11 h  F*O% h %V ."MFBPCR"\AS.seek 0.5ml F*Z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +21 h ."MFBPCR"delay 2 seconds +200 F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*P  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*}  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*m % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +140744 #"WCR"\Sampled 1200.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'~  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'\\ @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'g  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'k ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'+  'f  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'2  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'3  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'b' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)1% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'% ` +57 h ` ."WCR"\CS.seek 7.288ml C's' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'=  '9  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'5  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'E% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'8' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'*  +94 h  C'A% h % ."WCR"\CSV.dial between air and CTV C') ` +56 h ` ."WCR"\CS.seek 7.288ml C'7' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'<  'D  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'"  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&J% h %\  A(/% h %X A('. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (K(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h &( M&& h &ӷ M&M% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &o( +143 h ( M&>& h & A($& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +89 h ' A(?& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +253 h 'K M&V& h & A(Y& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +26 h 'C ."WCR"\Elbow.hold M&Q, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(^- h -+K M&$&.,(\ #b ."WCR"\SE.seek 1.04 Pucks below Top Plate A(@' +27 h ' M&[% h %C A(N& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&W'o +20 h 'o M&e& h & A(_& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &U' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(4' +37 h ' M&i& h & M&n% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  ."WCR"\Elbow.seek CC M&B(ӷ +143 h (ӷ M&`& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ ."WCR"\Forearm.seek CC M&a' +91 h ' ."WCR"Hand.close M&d/ +150 h / ."WCR"\CC.seek :open C'O( +415 h ( A(X& h &( A(Z& h &( M&]& h &ӷ M&t% h % ."WCR"\Forearm.seek :retract M&v'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &:( +143 h ( M&C& h & A(q& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &S'C +92 h 'C ."WCR"\Elbow.hold M&P, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &y1 +150 h 1 M&j% h %C A(}& h &( ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &r'o +20 h 'o M&T& h & A(m& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &H' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (x% h %B M&u% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&1 +2 A(6 h 1 M&\ & h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&z& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'g& h &W .tHand.open M&L1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'+& h &W C'f% h % .t\CSV.dial CSR C'  +38 h  C'2% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'b& h &W C' % h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'%% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'9  '=  +38 h  +113  .t\CBV.dial :waste1 C's @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'5% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C',  +38 h  .t\CBV.dial :waste2b C'*  +75 h  .t\CS.seek 0ml C'A'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C')  +38 h  C'&% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +709 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'" @ +75 h @ .t\CS.seek 0ml C'0'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  +38 h  .t\CBV.dial :waste2b C'F  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'M  +37 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '$'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6 'G  +38 h +75 .t\CTV.dial :air C'V  +151 h .t\CS.seek 0ml C'Y'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +506 h '0 .t\CSV.dial :waste C'[ +38 h .t\CS.seek 0ml C'N'4 +506 h '4 #t\Flushing upper manifold C'e& h &W C'_% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'o% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'`'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'@'4 +507 h '4 C'U& h &W C'Q% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.seek 3ml C'I'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'Z'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C']'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'C +76 h .t\CS.seek 0ml C'q'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'B% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 5ml C'j'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'T% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  '  +37 h  +113  .t\CBV.dial :waste1 C': @ +38 h @ .t\CS.seek 0ml C'x'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'u& h &W C'{% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'\ 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'S% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  +38 h  .t\CBV.dial :waste2b C'H  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&g1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&f1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &2(- +6 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&3% h %η .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'%(W +416 h (W C'& h &W .tHand.open M&=1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'% h %4 .t\CSV.dial CSR C'5  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '8'[ +463 h '[ .t\CSV.dial :waste C'E +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'*& h &W C'% h %4 .t\CSV.dial :flush C')  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +37 h @ C'<% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  +38 h  .t\CBV.dial :waste1 C'D @ +75 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'0% h %4 .t\CSV.dial :flush C'J  +76 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C'/% h %p .t\CS.seek 7ml C'M'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'>  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'$'4 +766 h '4 #tDisinfecting external Intake with kill C'G& h &W C'6% h %4 .t\CSV.dial CSR C'V  +38 h  C'?% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'Y'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'[' +416 h ' ."connect"\CSV.dial CBV C'^ .t\CBV.dial CSV C'e  +37 h +76 .t\CTV.dial :intake C'_  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'i' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'N  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +606 h '4 .t\CSV.dial CSR C'o  +113 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +1372 h ' .t\CSV.dial CBV C'` +113 h .t\CS.seek 0ml C'W'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&;/ +150 h / .t\CC.seek :open C'X( +416 h ( M&I& h &ӷ M&Z% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &C(- +264 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&y1 +150 h 1 M&q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +366910 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi$; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*T% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*4% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*r  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *P'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*9  +11 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F**  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*)' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*,  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*&  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*7  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*J n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*F'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*>' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*$% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*e  +21 h ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +65 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*v'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*j'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *K'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +10 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*L I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*+' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*f n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*1  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*2' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*5  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F**'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*,'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*)% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *N'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ARV.dial :carrier F*c  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*a  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*] n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*T I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*v'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*u  +107 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*{'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*m n +21 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.65ml F*4' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*K% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*+'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*\\% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +21 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *<'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*D'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *''Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*> I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*V% h %Fj ."MFBPCR"\AS.seek 0.524ml F*G' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*. % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*?' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*e  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +22 h  ."MFBPCR"delay 7 seconds +700 F*_% h %< ."MFBPCR"\AS.seek 0.594ml F*N'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*R  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*`' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*;'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*d @@T *s  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*%  +151 h F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *a' +423 h ' ."MFBPCR"\ATV.dial :ASV F*U n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*y  +43 h  F*@% h % ."MFBPCR"\AS.seek 0.62ml F*]'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*B n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *T'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*l'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*q % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*v% h % ."MFBPCR"\AS.seek 0.71ml F*m' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F* % h % Ű ."MFBPCR"\AS.seek 0.72ml F*S' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*r n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*4' +7161 h ' ."MFBPCR"\ATV.dial :waste F*P n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*~' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*f n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*g'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*\\ % +108 h % F*+% h %V ."MFBPCR"\AS.seek 0.01ml F*O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*b% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*1' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*= n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2  +21 h  ."MFBPCR"\AS.seek 0ml F*|'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*(  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *5' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *9'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*E  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b **' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*"  +42 h  F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*<% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *D'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *0' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*F I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*'  +64 h  F*.% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *$'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*G n +65 h n F*e% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *^' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*? I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*_  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*i n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *['Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*N n +150 h n ."MFBPCR"\AS.seek 0ml F*R'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*o  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*n % +43 h % F*!% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*`% h % ."MFBPCR"\AS.seek 0.96ml F*c'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %0 ."MFBPCR"\AS.seek 0.985ml F*;' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.97ml F*I'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*U I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *B'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *t'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*T  +43 h  F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *l' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +43 h I ."MFBPCR"\AS.seek 0.02ml F*:' +9880 h ' ."MFBPCR"\ATV.dial :waste F*j n +129 h n ."MFBPCR"\AS.seek 0ml F*v'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*u% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*{  +21 h ."MFBPCR"\ATV.dial :air3 F*m % +43 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*+% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *9'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:19:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:35.513058 #-> exit #m\EXIT @1536754844.59CEST @1536755347.86CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiP!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:43:24CET01-Jan-70 .delayUntil "16:43:24.00" +5660399 #-> CC !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Wcollection microcontroller never signed on while reading Collection Clamp's status:@checkpoint: omitted #-> ESP.configure .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ % *\ % 0\ C' -\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X exit #m\EXIT @1536755408.72CEST @1536755866.11CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:37:55, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:38:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ % *\ S)&\ C' -\ P% 0\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:38:30, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1200ml .BACfans.suppressMail "Started mfb in tube 2 at 14:38:35CEST12-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:38:30, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1200ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %8 .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*I  +11 h  ."readyMFB"\ASV.dial :waste1 F*H  h +912 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'K% h %4 S)J% h % S)M% h % .tIntake.select :close S)0 +6 )N% h % .t\SSV.dial :exhaust S)O  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9Td +100 C'99 h 9Xd +100 C'I9 h 9(\\d +100 C'H9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'G9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'x9 h 9 d +100 C';9 h 9 *d +100 C'q9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 & M&9& h &( &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(I'Z .t\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(G& h &( A(R& h &( M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +142 h ( M&W& h & A(S& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'C +93 h 'C .t\Elbow.hold M&T, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %C A(]& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&\\'o +20 h 'o M&^& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +23 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast #Tranferring lysate to PRV A(5 .t\CSV.dial :air C'f @ +2 A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +18 h ` .t\AIV.dial :bypass C'e { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P%| +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'\  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A(!% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ($& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A()& h &  .t\SE.seek 19.71 Pucks below Top Plate A( ' +66 h ' A(&& h &  M&8& h & .t\Elbow.seek 2 M&=( +6 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down M&B& A(:'X h & M&D% h %8 .t\Forearm.seek :retract M&E'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&F(ӷ +143 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &9' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +151 h  .t\PS.seek :empty P%Y' +2609 h ' .t\CBV.dial :waste1 C'] @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +37 h @ .t\CS.seek 10ml C'c' +2648 h ' .t\CSV.dial CTV C'Z  +37 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +151 h C'I& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h &( M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +143 h ( M&o& h & A(b& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &A' +89 h ' A(6& h &( .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +264 h '\\ M&r& h & A(C& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'C +26 h 'C .t\Elbow.hold M&a, +6 h , .t\Elbow.reconfigure TightElbow A(j- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&q% h %C A(p& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&u'o +20 h 'o M&O& h & A(t& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +415 h ( A(& h &( A( & h &( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +143 h ( M& & h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%,' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%!  +19 h  .t\PRV.dial :air P%2  +19 h  P%$% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M&)& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&B% h %η .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C' & h &W .tHand.open M&:1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%9' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1094 h ' .t\PSV.dial :air P%g  +38 h  .t\PS.seek 0ml P%G' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +76 h .tdelay 2 seconds +200 P%H% h %r .t\PS.seek 0.475ml P%[' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +75 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +76 h @ .t\PS.seek 0ml P%Q' +2609 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +75 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +7 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'6 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %F' +1313 h ' .t\PSV.dial :air P%"  +37 h  .t\PS.seek 2.5ml P%r' +2610 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'q& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*O @@T .t\AWV.select :air F*t  .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*x I +42 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%s' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +112 h P%!% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +38 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %)'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%'  +112 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*@  +43 h  F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *D'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F* % h %Q ."MFBPCR"\AS.seek 0.01ml F*:' +171 h ' .t\PSV.dial :mixloop P%= +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*>'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*9% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  'M  +37 h  +113  .t\CBV.dial :waste1 C'H @ +38 h @ .t\CS.seek 0ml C'S'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'I' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +7 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&6/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&F% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1200ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (A( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +90 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&x% h %8 ."WCR"\Forearm.seek :retract M&{'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +30 h '\  ."WCR"\SC.coast #collectSample 1200ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +6 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteR F*9  +21 h  ."MFBPCR"\AS.seek 0.3ml F*+': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*%'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*R % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*U  +87 h F*G% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *M'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*H I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*W'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*[ n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*L  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *]% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *g' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *S% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *l' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*_% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *^' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*Q% h % ."MFBPCR"\AS.seek 0.279ml F*\\'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Z n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*f % +43 h % F*I% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *i'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*m I +21 h I F*V% h %v ."MFBPCR"\AS.seek 0.059ml F*o'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*6 % +21 h % F*5% h %H ."MFBPCR"\AS.seek 0.069ml F*E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*"% h % ."MFBPCR"\AS.seek 0.769ml F*F' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h % q ."MFBPCR"\AS.seek 0.794ml F*p' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*C I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *`' +4416 h ' ."MFBPCR"\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.seek :empty F*'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*t% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*j  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *x'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*; % +108 h % F*y% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*w n +108 h n ."MFBPCR"\ASV.dial :wasteR F*}% h %  ."MFBPCR"\AS.seek 0.09ml F*?' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*{' +625 h ' ."MFBPCR"\ATV.dial :air3 F*b % +21 h % F*c% h % ."MFBPCR"\AS.seek 0.13ml F*v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*~% h % ."MFBPCR"\AS.seek 0.23ml F*Y' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*A% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*! n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *2'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +85 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*4'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F**% h %(A ."MFBPCR"\AS.seek 0.6ml F*1'\  +1031 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*@% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *8' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*B % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*\  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*X' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*H % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +22 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +112240 #"WCR"\Sampled 1200.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +94 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'E ` +19 h ` ."WCR"\CS.seek 7.288ml C'h' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'q  'F  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +57 h ` ."WCR"\CS.seek 7.288ml C'K' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +76 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'x  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +151 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&& h & A(!& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A($'K +253 h 'K M&/& h & A(2& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'C +26 h 'C ."WCR"\Elbow.hold M&,, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&)% h %C A(4& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&0'o +20 h 'o M&3& h & A(*& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(<' +37 h ' M&1& h & M&@% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&B(ӷ +143 h (ӷ M&8& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'D( +415 h ( A(& h &( A(&& h &( M&(& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &:( +143 h ( M&& h & A(9& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&>% h %C A(U& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&G'o +20 h 'o M&%& h & A(H& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 +2 A(6 h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'F& h &W C'q% h % .t\CSV.dial CSR C'h  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'}  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C'? @ +75 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'c  +38 h  C'{% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +710 h ' ."connect"\CSV.dial CTV C'v  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'Y'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +37 h +75 .t\CTV.dial :air C'/  +151 h .t\CS.seek 0ml C'2'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C') +38 h .t\CS.seek 0ml C'4'4 +507 h '4 #t\Flushing upper manifold C'3& h &W C'*% h %4 .t\CSV.dial :flush C'1  +37 h  .t\CS.seek 5ml C'@'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'A% h %p .t\CS.seek 7ml C'$'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k +113 h .t\CTV.dial :air .\CS.seek 0ml C'8'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C'0 +37 h .t\CS.seek 0ml C''4 +507 h '4 C''& h &W C',% h %4 .t\CSV.dial :flush C'=  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'&'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'('4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'9'4 +506 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'B% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'%% h %p .t\CS.seek 7ml C'H'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  'N  +37 h  +113  .t\CBV.dial :waste1 C': @ +38 h @ .t\CS.seek 0ml C'L'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'M& h &W C'S% h %4 .t\CSV.dial :flush C']  +75 h  .t\CS.seek 5ml C'_'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C'% h %p .t\CS.seek 7ml C'G'i +416 h 'i ."connect"\CSV.dial CTV C'<  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'^'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&Z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'P( +415 h ( M&W& h &ӷ M&\\% h % .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &e(- +265 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &D'η +91 h 'η .tHand.open M&.1 +150 h 1 M&"% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'F& h & .tHand.open M&q1 +2 h 1 M&h& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&C'η +91 h 'η .tHand.close M&d/ +150 h / M&T& h &- M&% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'|(W +416 h (W C'n& h &W .tHand.open M& 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +38 h  C'j% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'y'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'x& h &W C';% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  +38 h  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'c'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'~% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'Y  +75 h  .t\CS.seek 0ml C'!'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'% h %4 .t\CSV.dial CSR C'/  +38 h  C'z% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'2'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C')' +416 h ' ."connect"\CSV.dial CBV C'7 .t\CBV.dial CSV C'3  +37 h +76 .t\CTV.dial :intake C'*  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'1' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'4  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 .t\CSV.dial CSR C'A  +113 h  C'@% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'k'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'$' +1372 h ' .t\CSV.dial CBV C'8 +113 h .t\CS.seek 0ml C'0'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&\ & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +7 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&&% h % .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&R1 +150 h 1 M&9% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +395414 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*%% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *H'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +21 h F*S% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*<% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Z  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*f  +32 h  ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*h'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *T'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*u% h %V ."MFBPCR"\AS.seek 0.5ml F*a'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*r  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*x  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*;% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*y  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*{% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*~  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*!% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*3  +21 h ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *)'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*A'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*k'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*$% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*=  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *`'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*M  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*+  +10 h  F*Q% h %V ."MFBPCR"\AS.seek 0.5ml F*V'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*o n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*g  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *.'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*" I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*q n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*5' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*b  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.536ml F*d'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +107 h  ."MFBPCR"delay 7 seconds +700 F*T% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*P  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*n % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h25ml.pcr F*a% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +199 .\ATV.dial :bleach F*  +21 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*x'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *{'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*2  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0111h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +21 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*% I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*O' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*N % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +107 h  ."MFBPCR"delay 7 seconds +700 F*>% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*]  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0111h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +86 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*\\% h %Z ."MFBPCR"\AS.seek 0.81ml F*f'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*i% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*b  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*p  +32 h  ."MFBPCR"\ATV.dial :air3 F*C % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*u'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*{% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*/% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*s % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*3  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*2  +22 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*4'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*1 n +22 h n ."MFBPCR"delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*8' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*` % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*= @@T *K  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*|  +151 h F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :ASV F*' n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*R  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*('\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*B n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *%'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*I'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*9 % +43 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +424 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +21 h F*X% h % ."MFBPCR"\AS.seek 0.71ml F*H' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h % H ."MFBPCR"\AS.seek 0.735ml F*]' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F*l% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*G n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Z% h % zG ."MFBPCR"\AS.seek 0.365ml F*<' +7161 h ' ."MFBPCR"\ATV.dial :waste F*+ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*W% h % ."MFBPCR"\AS.seek 0.579ml F*V' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +21 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %  ."MFBPCR"\AS.seek 0.604ml F*g'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +21 h F*[% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*f' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*q n +86 h n ."MFBPCR"\ASV.dial :wasteR F*i  +22 h  ."MFBPCR"\AS.seek 0ml F*.'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *h'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*5 % +108 h % F*F% h %V ."MFBPCR"\AS.seek 0.01ml F*D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*d% h % ."MFBPCR"\AS.seek 0.58ml F*e' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*p'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +21 h F* % h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*P'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *a'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*u  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b * '^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*j n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *r'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*^'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*;  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *x' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*w I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*?  +42 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*{  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*c n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*~  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b */': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*v  +64 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *!'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*3% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *7' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*z I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F**  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*1 n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *)'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*4 n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*A  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*@ % +43 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*8% h % ."MFBPCR"\AS.seek 0.96ml F*\ '0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*&% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*' I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *B'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *J'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*%  +43 h  F*9% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *I' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F*:' +9880 h ' ."MFBPCR"\ATV.dial :waste F*> n +129 h n ."MFBPCR"\AS.seek 0ml F*X'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*M% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*S  +21 h ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *l'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*Z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*6  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*W  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*i  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +86 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +21 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*h'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*r % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@14:39:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:39.361481 #-> exit #m\EXIT @1536756077.33CEST @1536848641.03CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:24:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:24:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ F*\ 9\ .t\SPE.coast P% 0\ C' 2\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:24:27, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 400ml, wcr at most 400ml .BACfans.suppressMail "Started mfb in tube 2 at 16:24:27CEST13-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:24:27, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 400ml, wcr at most 400ml" F*/% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&I& A(H'X h & M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 400ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'*9 h 9 d +100 C'9 h 9 *d +100 C'+9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'H9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'!9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'$ @ +37 h @ .t\CS.seek 5ml C'Z'p +1373 h 'p .t\CSV.dial CTV C'[  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'a +113 h .t\CBV.dial CSV C'4  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +37 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +132 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (i(( +200 h (( .t\SC.reconfigure SCloose M&`& A($.(\ 1~b (o& h &ӷ &( .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(q'Z .t\Elbow.seek CC M&$&.,(\ #b &r(ӷ +7 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +392 ( A(n& h &( A(y& h &( M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A({& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +165 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +150 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +37 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%8' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C':  P%9  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%=  +113 h .t\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%<' +2609 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%E  +37 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.seek 0ml P%I' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&R% h %\  A(!% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (T( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ($& h &  M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +166 h ( M&W& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(a& h &  .t\SE.seek 19.71 Pucks below Top Plate A(4' +66 h ' A(]& h &  M&_& h & .t\Elbow.seek 2 M&d( +6 h ( M&e& h & A(c& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &X/ +150 h / ."downElevator"\SE.seek :down A(f'X M&j& h & M&k% h %8 .t\Forearm.seek :retract M&l'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +143 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%{ +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +18 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &i' +90 h ' A(b& h &( .t\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +25 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&+% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&('o +21 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +36 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +142 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C':( +416 h ( A(0& h &( A(=& h &( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&<& h & A(E& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &D'C +92 h 'C .t\Elbow.hold M&B, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h %C A(&& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&J'o +21 h 'o M&>& h & A(*& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  +60 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%K  +2 A(6 +111 h  P%G% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +38 h .t\PRV.dial :air P%F  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'R  +38 h .t\PRV.dial between CBV and air P%!  +18 h  .t\PRV.dial :air P%Y  +19 h  P%$% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +251 h ' .t\PRV.dial :waste2 P%[ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&^1 +2 h 1 M&a& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&X& h &- M&j% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'4& h &W .tHand.open M&f1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%o' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%n' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +37 h  .tdelay 2 seconds +200 P%I% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +75 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1312 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '+& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass F*w @@T *  .t\PSV.dial PSR P%2 +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +38 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%8' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +37 h  .t\PS.seek 0ml P%6' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +21 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F** I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*?' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%K' +739 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%.' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2609 h ' .t\PSV.dial PSR P%R +113 h P%!% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +37 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%[ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%O' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %a'k +2679 h 'k .t\PSV.dial :waste1 P%@  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +849 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%X' +2610 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %H' +5106 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*4% h %Q ."MFBPCR"\AS.seek 0.01ml F*f' +171 h ' .t\PSV.dial :mixloop P%d +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*t n +128 h n ."MFBPCR"\AS.seek :empty F*`'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +37 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'I' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  'u  +38 h  +113  .t\CBV.dial :waste1 C'r @ +38 h @ .t\CS.seek 0ml C'{'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'|'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +107 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 400ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (i( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &  M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(=& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&0( +7 h ( M&E& h & A(5& h &  ."WCR"\Forearm.seek 2 + 300 counts M&)'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &8/ +150 h / ."downElevator"\SE.seek :down A(<'X M&&& h & M&*% h %8 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +142 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +4 ."MFBPCR"\ATV.dial :waste2 F*D % +42 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +29 h '\  ."WCR"\SC.coast #collectSample 400ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)?+ +7 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'M @ +75 h @ +50 S)i% h % ."WCR"\CBV.dial between CSV and waste2b C'?  +95 h  C'R% h % ."WCR"\CSV.dial between air and CTV C'! ` +56 h ` ."WCR"\CS.seek 7.288ml C'V' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'$  'Y  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '.'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'Q  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +805 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*^ % +108 h % F*[% h %V ."MFBPCR"\AS.seek 0.59ml F*@' +3087 ."WCR"\CTV.dial :air,avoiding: :intake C'a  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'e  +37 h  C'W% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'U' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'O' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'X @ +76 h @ +50 S)c% h % ."WCR"\CBV.dial between CSV and waste2b C'H  +94 h  C'h% h % ."WCR"\CSV.dial between air and CTV C'_ ` +56 h ` ."WCR"\CS.seek 7.288ml C'j' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'B  ':  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"delay 2:00 +3848 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*d n +108 h n ."MFBPCR"\ASV.dial :wasteR F*k% h %  ."MFBPCR"\AS.seek 0.09ml F*]' +7744 ."WCR"\CBV.dial :waste2b C't  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'N' +41 h ' ."WCR"delay 1:00 +2274 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*S I +85 h I F*f% h % ."MFBPCR"\AS.seek 0.12ml F*C' +298 ."WCR"\CTV.dial :air,avoiding: :intake C'o  +151 h C'`& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&P% h %\  A(y% h %X A(T. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (}(( +177 h ' ."MFBPCR"\ATV.dial :air3 F*n % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*r' +2 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (I& h &( M&u& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &x( +143 h ( M&& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &{' +77 h ' ."MFBPCR"delay 2 seconds +12 h ' A(& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(\ 'K +188 ."MFBPCR"\ATV.dial :bleach F*  +22 h F*z% h % ."MFBPCR"\AS.seek 0.23ml F*' +44 h 'K M&& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C ."WCR"\Elbow.hold M&q, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(|- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&~% h %C A(& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&b'o +20 h 'o M&\\& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &g' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(L' +36 h ' M&l& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +89 h '\  ."WCR"\Elbow.seek CC M&m(ӷ +143 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'3( +416 h ( A(& h &( A(p& h &( M&(& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&1'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(%& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &s'C +93 h 'C ."WCR"\Elbow.hold M&=, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &91 +150 h 1 M&% h %C A(& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&5& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &E' +23 h ' A(&% h % ."downElevator"\SE.seek :down A(*'X +100 (% h %B M&,% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +89 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&)1 +2 A(6 h 1 M&"& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&0(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&D'η +26 h ' ."MFBPCR"delay 3 seconds +65 h 'η .tHand.close M& / +150 h / M& & h &- M&<% h %η .t\Forearm.seek :retract M&/'\ +85 ."MFBPCR"\ATV.dial :air3 F* % +6 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +16 h % ."MFBPCR"delay 3 seconds +249 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +51 F*i% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +40 h ' .t\CC.seek :closed C'R(W +416 h (W C'!& h &W .tHand.open M&>1 +69 h 'X ."MFBPCR"delay 3 seconds +81 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'V& h &W C'Q% h % .t\CSV.dial CSR C'.  +38 h  C'^% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'['p +85 ."MFBPCR"\ATV.dial :bleach F*J  +22 h F*a% h %X ."MFBPCR"\AS.seek 0.24ml F*e' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*W n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +329 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'X% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'c'[ +463 h '[ .t\CSV.dial :waste C'H +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'h'4 +71 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *O'5 +1833 h '4 #t\Flushing lower manifold's waste1 port C':& h &W C'B% h %4 .t\CSV.dial :flush C'j  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'd'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'@% h %p .t\CS.seek 7ml C't'i +416 h 'i ."connect"\CSV.dial CTV C'4  .t\CTV.dial CSV C'v  +37 h  +113  .t\CBV.dial :waste1 C'S @ +76 h @ .t\CS.seek 0ml C'f'4 +765 h '4 #t\Flushing lower manifold's waste2b port C']& h &W C'o% h %4 .t\CSV.dial :flush C'`  +76 h  .t\CS.seek 5ml C'P'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +38 h @ C'T% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'I  +76 h  .t\CS.seek 0ml C'u'4 +400 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F* @F@ h . +366 '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +710 h ' ."connect"\CSV.dial CTV C'6  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'z'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'G  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'~  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'\\'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'l% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CBV C'{ .t\CBV.dial CSV C'x  +37 h +76 .t\CTV.dial :air C'\  +150 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'b'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'g% h %4 .t\CSV.dial :flush C'q  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +37 h @ C';% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'p .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'('4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'%'4 +507 h '4 C'& h &W C'm% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %0 .t\CS.seek 5ml C''p +184 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +26 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C','4 +506 h '4 #t\Flushing lower manifold's waste1 port C'A& h &W C')% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C's @ +38 h @ C'% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  '8  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'}'4 +500 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F** I +60 h '4 #t\Flushing lower manifold's waste2b port C'=& h &W C' % h %4 .t\CSV.dial :flush C'  +26 h I ."MFBPCR"\AS.seek 0ml F*<'V +49 h  .t\CS.seek 5ml C'7'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'D% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'F  +38 h  .t\CBV.dial :waste2b C'0  +75 h  .t\CS.seek 0ml C'i'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&/& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&Y/ +150 h / .t\CC.seek :open C'V( +416 h ( M&Q& h &ӷ M&.% h % .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &J(- +264 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &N'η +91 h 'η .tHand.open M&?1 +150 h 1 M&W% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&X1 +2 h 1 M&[& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &H(- +6 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&U'η +91 h 'η .tHand.close M&:/ +150 h / M&B& h &- M&j% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +265 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &4' +91 h ' .t\CC.seek :closed C'v(W +416 h (W C'S& h &W .tHand.open M&t1 +150 h 1 M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'`& h &W C'f% h %4 .t\CSV.dial CSR C'y  +38 h  C'T% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'P'p +492 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +675 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +1105 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*u  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *6'\  +756 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'C% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +238 h '\  F*z% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +412 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +38 h @ C'% h %p .t\CS.seek 7ml C'|'i +81 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*l'\  +335 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\\  +37 h  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'x'4 +584 h '\  F*{% h %\  ."MFBPCR"\AS.seek 0.5ml F*\ '(B +182 h '4 #t\Flushing lower manifold's waste2b port C'r& h &W C'% h %4 .t\CSV.dial :flush C'2  +75 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +124 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *g' M +76 .t\CSV.dial :air C'q @ +38 h @ C'b% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C';  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'p'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'(% h %4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'm' +416 h ' ."connect"\CSV.dial CBV C'9 .t\CBV.dial CSV C'%  +37 h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'5' +338 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +135 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +607 h '4 .t\CSV.dial CSR C'  +112 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'A'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C')' +651 h 'V ."MFBPCR"\SPE.coast F*"  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*,  +64 h  ."MFBPCR"\ARV.dial :carrier F*s  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +647 h ' .t\CSV.dial CBV C'E +112 h .t\CS.seek 0ml C'8'4 +1788 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +654 h '4 #tGaraging FlushPuck in Collection Clamp M&L& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +6 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'}( +416 h ( M&& h &ӷ M&D% h % .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &F(- +264 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &/'η +91 h 'η .tHand.open M&31 +150 h 1 M&!% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +895 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*K n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *a'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*M  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*e' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*X % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*_'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*:'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*B n +21 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*@' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*d  +43 h  ."MFBPCR"\ASV.dial :waste1 F*H  +65 h ."MFBPCR"\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h25ml.pcr F*`% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *i'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*I% h %Z ."MFBPCR"\AS.seek 0.81ml F*P'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*u'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*C  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +64 h  F*z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *{' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*2'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0105h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +21 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *,'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*s  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*E  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*L  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F**'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +108 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.65ml F*'' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*3  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*!% h % \\ ."MFBPCR"\AS.seek 0.326ml F*7'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*K % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0105h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +21 h F*[% h %n ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*N  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *B'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +31 h  ."MFBPCR"\ATV.dial :air3 F*h % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*y'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*4% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*<  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0107h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*6% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*|  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*{% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*x n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.53ml F*q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*b  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +108 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*+ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*('\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*p' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*%  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*m'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0107h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*g  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*,% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *k'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +21 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*K'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*7'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0109h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*j'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +21 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*t n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*]% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*` I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*T % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.536ml F*y'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*P  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*u  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*C  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0109h25ml.pcr F*z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*\\'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*b  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*( % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*m'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*5  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0112h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ARV.dial :carrier F*L  +10 h  F*k% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *0'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*' I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*/% h %Fj ."MFBPCR"\AS.seek 0.524ml F*!' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*K' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.536ml F*7'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +22 h  ."MFBPCR"delay 7 seconds +700 F*>% h %< ."MFBPCR"\AS.seek 0.594ml F*M'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*^  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*X' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*?  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*_'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*B % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0112h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*N @@T *j  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*:  +151 h F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *H' +423 h ' ."MFBPCR"\ATV.dial :ASV F*h n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*V  +43 h  F*J% h % ."MFBPCR"\AS.seek 0.62ml F*t'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*] n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *`'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*S'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*@ % +43 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *4' +423 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*<' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +21 h F*y% h % ."MFBPCR"\AS.seek 0.71ml F*i' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h % H ."MFBPCR"\AS.seek 0.735ml F*P' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h % Ű ."MFBPCR"\AS.seek 0.72ml F*u' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*C n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*G% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*z % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*~' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*{% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*\\' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*x n +86 h n ."MFBPCR"\ASV.dial :wasteR F*2  +22 h  ."MFBPCR"\AS.seek 0ml F*r'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*q % +108 h % F*l% h %V ."MFBPCR"\AS.seek 0.01ml F*\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*+' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F* '\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*p  +21 h  ."MFBPCR"\AS.seek 0ml F*%'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *g' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *m'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*5 n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +202 h 'R ."MFBPCR"\SPE.coast F*"  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*A n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *s' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*) I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *L': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*k  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b **'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*v% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *o' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*D I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b */': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*0  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*! n +65 h n F*Q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *.' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*K I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*>  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *7': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *R'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*M n +151 h n ."MFBPCR"\AS.seek 0ml F*^'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*a  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*W % +43 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*X% h % ."MFBPCR"\AS.seek 0.96ml F*e'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %0 ."MFBPCR"\AS.seek 0.985ml F*_' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*U% h % ."MFBPCR"\AS.seek 0.97ml F*'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*h I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *J'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *]'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*`  +43 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *S' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +43 h I ."MFBPCR"\AS.seek 0.02ml F*4' +9880 h ' ."MFBPCR"\ATV.dial :waste F*f n +129 h n ."MFBPCR"\AS.seek 0ml F*y'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*<% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*I  +22 h ."MFBPCR"\ATV.dial :air3 F*i % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *n'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*C% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *|'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*2  +32 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*l% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*+'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *m'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*1 % +108 h % ."MFBPCR"\AS.seek 0ml F*A'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:25:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:33.854309 #-> exit #m\EXIT @1536848821.74CEST @1536940849.19CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:00:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:01:17, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:01:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 200ml, wcr at most 200ml .BACfans.suppressMail "Started mfb in tube 2 at 18:01:22CEST14-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:01:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" F*/% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&I& A(H'X h & M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 200ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'+9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'H9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'$9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'a +113 h .t\CBV.dial CSV C'4  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +38 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +131 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (i(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b M&`& A(o& h &ӷ &( .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit .t\Elbow.seek CC M&$&.,(\ #b A(q'Z M&r(ӷ +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(n& h &( A(y& h &( M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%8' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C':  P%9  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%=  +113 h .t\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%B +37 h .t\PS.seek 0ml P%I' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +90 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +265 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&R% h %\  A($% h %X A(X. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (T( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +166 h ( M&W& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +89 h ' A(a& h &  .t\SE.seek 19.71 Pucks below Top Plate A(4' +66 h ' A(]& h &  M&_& h & .t\Elbow.seek 2 M&d( +7 h ( M&e& h & A(c& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down M&f& A(j'X h & M&k% h %8 .t\Forearm.seek :retract M&l'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +142 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +151 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h &( .t\SE.seek -0.29 Pucks below Top Plate A('\\ +265 h '\\ M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +25 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +26 h ' M&*% h %C A(& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &('o +21 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +36 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C':( +416 h ( A(0& h &( A(=& h &( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +142 h ( M&<& h & A(E& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &D'C +93 h 'C .t\Elbow.hold M&B, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h %C A(&& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&J'o +21 h 'o M&>& h & A(+& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%K  +2 A(6 +111 h  P%G% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +37 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'R  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%X  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&^1 +2 h 1 M&a& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&Y& h &- M&f% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +90 h ' .t\CC.seek :closed C'g(W +416 h (W C'4& h &W .tHand.open M&j1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%o' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%n' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%I% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +75 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1313 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '*& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*w @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%2 +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%i' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%8' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%6' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*?' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2609 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +37 h  .t\PS.seek 0ml P%K' +740 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%.' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %a'k +2678 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .t\PSV.dial :mixloop P%f +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %H' +5107 h ' .t\PSV.dial :air P%c  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*4% h %Q ."MFBPCR"\AS.seek 0.01ml F*j' +171 h ' .t\PSV.dial :mixloop P%d +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*`'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'I' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'u  'n  +38 h  +113  .t\CBV.dial :waste1 C'r @ +37 h @ .t\CS.seek 0ml C'{'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 200ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (i( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &  M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(=& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&0( +6 h ( M&E& h & A(5& h &  ."WCR"\Forearm.seek 2 + 300 counts M&)'8 +23 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &8/ +150 h / ."downElevator"\SE.seek :down A(<'X M&&& h & M&+% h %8 ."WCR"\Forearm.seek :retract M&'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +142 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +29 h '\  ."WCR"\SC.coast #collectSample 200ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)?+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % q ."MFBPCR"\AS.seek 0.794ml F*M' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*?% h % k ."MFBPCR"\AS.seek 0.779ml F*$'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*R I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *V' +4416 h ' ."MFBPCR"\ATV.dial :waste F*! n +128 h n ."MFBPCR"\AS.seek :empty F*X'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*[% h % ."MFBPCR"\AS.seek 0.579ml F*@' +4172 ."WCR"\CBV.dial :waste2b C'a  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'e' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h C'U& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&O% h %\  A(Y% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (H(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (h& h &( M&_& h &ӷ M&f% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &B( +143 h ( M&:& h & A(4& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +89 h ' A(k& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(]'K +254 h 'K M&t& h & A(N& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &v'C +25 h 'C ."WCR"\Elbow.hold M&S, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(j- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(C' +26 h ' M&o% h %C A(`& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&P'o +21 h 'o M&y& h & A(T& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(n' +36 h ' M&& h & M&r% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  ."WCR"\Elbow.seek CC M&u(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ ."WCR"\Forearm.seek CC M&' +46 h '  ."MFBPCR"delay 3 seconds +45 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'{( +105 ."MFBPCR"\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +289 h ( A(\ & h &( A( & h &( M&z& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&'\ +11 F*|% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +80 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &q( +142 h ( M&& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\\1 +150 h 1 M&% h %C A(l& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&~'o +21 h 'o M&L& h & A(g& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(*% h % ."downElevator"\SE.seek :down A('X +9 h '\ 48 ."MFBPCR"delay 3 seconds +91 A(m% h %B M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 + A(6 + h 1 M&& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +66 ."MFBPCR"\ATV.dial :bleach F*%  +22 h F*(% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*p' +78 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&s'η +91 h 'η .tHand.close M&=/ +150 h / M&9& h &- M&% h %η .t\Forearm.seek :retract M&'\ +4 h '  ."MFBPCR"delay 3 seconds +86 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +214 ."MFBPCR"\ATV.dial :ASV F*5 n +51 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +35 h n ."MFBPCR"\ASV.dial :wasteR F*+  +43 h  ."MFBPCR"\AS.seek 0ml F*8'V +13 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&A1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'7& h &W C'D% h % .t\CSV.dial CSR C'0  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '''[ +463 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'/& h &W C'J% h %4 .t\CSV.dial :flush C'F  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'i% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CTV C'M  .t\CTV.dial CSV C'R  +37 h  +113  .t\CBV.dial :waste1 C'$ @ +76 h @ .t\CS.seek 0ml C'!'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'.& h &W C'Q% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'X'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +38 h @ C'[% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'a  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'K  +76 h  .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'U  +37 h  C'O% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Y'P +1116 h 'P .tdelay 5 seconds +304 h 'V ."MFBPCR"delay 1:00 +196 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +37 h  .t\CBV.dial :waste1 C'_ @ +76 h @ .t\CS.seek 0ml C'f'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C':  +38 h  C'4% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'k'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +709 h ' ."connect"\CSV.dial CTV C'G  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'N'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'j  +38 h  C'o% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '`'P +623 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +287 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r '  +38 h +75 .t\CTV.dial :air C'd  +150 h .t\CS.seek 0ml C'B'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C'v +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'x& h &W C'P% h %4 .t\CSV.dial :flush C'C  +38 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +37 h @ C'% h %p .t\CS.seek 7ml C'I'i +119 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +91 h 'i ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'z +38 h .t\CS.seek 0ml C''4 +506 h '4 C'|& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'\\'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C'g'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'*& h &W C'% h %4 .t\CSV.dial :flush C'q  +37 h  .t\CS.seek 5ml C'm'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'1  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'~'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'n& h &W C'%% h %4 .t\CSV.dial :flush C'(  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'H  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'E( +416 h ( M&5& h &ӷ M&2% h % .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&A1 +150 h 1 M&)% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&01 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +7 h (- M&e& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&(W +416 h (W C'M& h &W .tHand.open M&R1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'.& h &W C'Q% h %4 .t\CSV.dial CSR C'V  +37 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '^'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '[% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'X'[ +463 h '[ .t\CSV.dial :waste C'a +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'K'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'U% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'W'p +650 h 'p .tdelay 5 seconds +384 h 'V ."MFBPCR"delay 20 seconds +116 .t\CSV.dial :air C'Y @ +38 h @ C'h% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  +37 h  .t\CBV.dial :waste1 C': @ +76 h @ .t\CS.seek 0ml C'4'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'f& h &W C'k% h %4 .t\CSV.dial :flush C'G  +75 h  .t\CS.seek 5ml C't'p +476 ."MFBPCR"\ATV.dial :air3 F*6 % +107 h % F*j% h %V ."MFBPCR"\AS.seek 0.59ml F*o' +67 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C'8% h %p .t\CS.seek 7ml C'`'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'r  +75 h  .t\CS.seek 0ml C'd'4 +766 h '4 #tDisinfecting external Intake with kill C'T& h &W C'B% h %4 .t\CSV.dial CSR C'v  +37 h  C']% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'x'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'P' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'C  +38 h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'}  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ';'4 +607 h '4 .t\CSV.dial CSR C'y  +113 h  C'I% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'\ 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'z' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'|'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'\\( +129 h '  ."MFBPCR"delay 3 seconds +287 h ( M&& h &ӷ M&L% h % .t\Forearm.seek :retract M&l'\ +13 ."MFBPCR"\ATV.dial :ASV F** n +78 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +29 h n ."MFBPCR"\ASV.dial :wasteR F*q% h %  ."MFBPCR"\AS.seek 0.09ml F*g' +236 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&11 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +9510 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*n I +86 h I F*%% h % ."MFBPCR"\AS.seek 0.12ml F*(' +625 h ' ."MFBPCR"\ATV.dial :air3 F*~ % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*H% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*=% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*" n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *s'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*p @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*9 I +86 h I ."MFBPCR"\AS.seek 0ml F*A'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*)'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*+  +43 h  #"MFBPCR"\Swish #1 of 2 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *0'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*e'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*/% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*7 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +65 h  ."MFBPCR"\ARV.dial :carrier F*&  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*E'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*^' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*a  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*U % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.536ml F*K'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +107 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*c n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*4  +43 h  ."MFBPCR"\ASV.dial :waste1 F*6  +64 h ."MFBPCR"\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*`  +21 h ."MFBPCR"\ATV.dial :air3 F*T % +87 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*x'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F**% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*o% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0105h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*=% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*,  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *5'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*s  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*A  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*D' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*)  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*&  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*J% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*R % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0105h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *.'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*^'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *c'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*j % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*`'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*B% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0107h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*?  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F** I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*u' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*o  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*l% h %< ."MFBPCR"\AS.seek 0.594ml F*m'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*n% h %\  ."MFBPCR"\AS.seek 0.6ml F*%'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*(  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*H % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0107h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*3  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*"% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +21 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*D  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*R'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +21 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +21 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*!'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0109h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*:'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*f n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*j  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*8 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*G' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*T % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*`'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +108 h  ."MFBPCR"delay 7 seconds +700 F*r% h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +21 h n ."MFBPCR"delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*I% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0109h25ml.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*q% h %n ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*o  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +31 h  ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F*(% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*='> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0112h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*)  +11 h  F*e% h %V ."MFBPCR"\AS.seek 0.5ml F*0'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*J' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*p % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*R' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +22 h  ."MFBPCR"delay 7 seconds +700 F*!% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*[ n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*^  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*K'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*c % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0112h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*h @@T ."MFBPCR"\ASV.dial :SPEdelivery F*:  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*W  +150 h F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *6' +424 h ' ."MFBPCR"\ATV.dial :ASV F*j n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*_% h % ."MFBPCR"\AS.seek 0.62ml F*8'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*t n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*G % +43 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *B' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*`% h % ."MFBPCR"\AS.seek 0.71ml F*v' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h % H ."MFBPCR"\AS.seek 0.735ml F*x' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*S' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*I% h % zG ."MFBPCR"\AS.seek 0.365ml F*}' +7162 h ' ."MFBPCR"\ATV.dial :waste F*\ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*; % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*@% h % ."MFBPCR"\AS.seek 0.579ml F*z' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*?'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*F' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*q  +22 h  ."MFBPCR"\AS.seek 0ml F*L'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*u% h %V ."MFBPCR"\AS.seek 0.01ml F*1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F* ' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h % q ."MFBPCR"\AS.seek 0.605ml F*~'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*(% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*g' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*b n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*=  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *3' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +202 h 'R ."MFBPCR"\SPE.coast F*2  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*9  +43 h  F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *s' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *)': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*e  +65 h  F*D% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *0'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*/ n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*7  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *E': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*p% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *M'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*J n +65 h n F*Q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *V' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*R I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*!  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*[ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *.'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*X'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*i  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*a % +43 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*U% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %0 ."MFBPCR"\AS.seek 0.985ml F*K' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*k% h % ."MFBPCR"\AS.seek 0.97ml F*f'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*j I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *t'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *4'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*G% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *N' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +42 h I ."MFBPCR"\AS.seek 0.02ml F*B' +9880 h ' ."MFBPCR"\ATV.dial :waste F*r n +129 h n ."MFBPCR"\AS.seek 0ml F*`'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*]% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*v % +43 h % F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*I'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +21 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*@  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +31 h  ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*u% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*1'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +21 h F*m% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *3'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*2 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*, % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:34.198447 #-> exit #m\EXIT @1536941056.22CEST @1537039061.48CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refit!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun1.rb" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@21:17:50, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@21:18:59, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@21:19:04, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun1 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 200ml, wcr at most 200ml .BACfans.suppressMail "Started mfb in tube 2 at 21:19:04CEST15-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@21:19:04, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" F*/% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil M&11 F* +6 *2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h & M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 200ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C',9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C'+9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'-9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'$9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'a +113 h .t\CBV.dial CSV C'4  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +38 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +131 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (i(( +201 h (( .t\SC.reconfigure SCloose M&`& A($.(\ 1~b h &ӷ A(o& .t\Elbow.reconfigure TightElbow h &( .t\Elbow.seek CC ."raiseStack"\SE.seek :upperLimit M&$&.,(\ #b &q(ӷ A(r'Z +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(n& h &( A(y& h &( M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&& h & A(x& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%8' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%9  C':  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%=  +113 h .t\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%B +37 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +90 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +265 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'V& h & M&R% h %\  A($% h %X A(X. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (T( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\\( +166 h ( M&W& h & A(^& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +89 h ' A(a& h &  .t\SE.seek 19.71 Pucks below Top Plate A(4' +66 h ' A(]& h &  M&_& h & .t\Elbow.seek 2 M&d( +7 h ( M&e& h & A(c& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down M&f& A(j'X h & M&k% h %8 .t\Forearm.seek :retract M&l'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +142 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +151 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%q +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'r& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h &( .t\SE.seek -0.29 Pucks below Top Plate A('\\ +265 h '\\ M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +25 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +26 h ' M&*% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&('o +21 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +36 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C':( +416 h ( A(0& h &( A(=& h &( M&8& h &ӷ M&% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +142 h ( M&<& h & A(E& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &D'C +93 h 'C .t\Elbow.hold M&B, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h %C A(&& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&J'o +21 h 'o M&>& h & A(,& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  +2 A(6 +111 h  P%G% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%- +37 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'R  +37 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%X  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&^1 +2 h 1 M&a& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&Y& h &- M&f% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +90 h ' .t\CC.seek :closed C'g(W +416 h (W C'4& h &W .tHand.open M&j1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%o' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%n' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +75 h .tdelay 2 seconds +200 P%q% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%r  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1313 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '*& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& F*w @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%i' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +22 h ' .t\PSV.dial PSR P%+ +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%8' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%6' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F*, I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*?' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2609 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +37 h  .t\PS.seek 0ml P%K' +740 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%-' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +113 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%^ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %a'k +2678 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .t\PSV.dial :mixloop P%f +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5107 h ' .t\PSV.dial :air P%c  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*4% h %Q ."MFBPCR"\AS.seek 0.01ml F*j' +171 h ' .t\PSV.dial :mixloop P%d +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*`'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +1625 h ' ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV C'u  +38 h  +113  .t\CBV.dial :waste1 C'q @ +37 h @ .t\CS.seek 0ml C'{'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'r' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 200ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (i( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &  M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&9& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(=& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&0( +6 h ( M&E& h & A(5& h &  ."WCR"\Forearm.seek 2 + 300 counts M&)'8 +23 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &8/ +150 h / ."downElevator"\SE.seek :down A(<'X M&&& h & M&,% h %8 ."WCR"\Forearm.seek :retract M&'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +142 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +29 h '\  ."WCR"\SC.coast #collectSample 200ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)?+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % q ."MFBPCR"\AS.seek 0.794ml F*M' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*?% h % k ."MFBPCR"\AS.seek 0.779ml F*$'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*R I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *V' +4416 h ' ."MFBPCR"\ATV.dial :waste F*! n +128 h n ."MFBPCR"\AS.seek :empty F*X'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*- % +43 h % F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*[% h % ."MFBPCR"\AS.seek 0.579ml F*@' +4172 ."WCR"\CBV.dial :waste2b C'a  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'e' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h C'U& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&O% h %\  A(Y% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (I(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (h& h &( M&_& h &ӷ M&f% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &B( +143 h ( M&:& h & A(4& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +89 h ' A(k& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(]'K +254 h 'K M&t& h & A(N& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &v'C +25 h 'C ."WCR"\Elbow.hold M&S, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(j- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(C' +26 h ' M&o% h %C A(`& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&P'o +21 h 'o M&y& h & A(T& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(n' +36 h ' M&& h & M&q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  ."WCR"\Elbow.seek CC M&u(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ ."WCR"\Forearm.seek CC M&' +46 h '  ."MFBPCR"delay 3 seconds +45 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'{( +105 ."MFBPCR"\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +289 h ( A(\ & h &( A( & h &( M&z& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&'\ +11 F*|% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +80 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &r( +142 h ( M&& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &l1 +150 h 1 M&% h %C A(\\& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&~'o +21 h 'o M&L& h & A(g& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(*% h % ."downElevator"\SE.seek :down A('X +9 h '\ 48 ."MFBPCR"delay 3 seconds +91 A(m% h %B M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 + A(6 + h 1 M&& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +66 ."MFBPCR"\ATV.dial :bleach F*%  +22 h F*(% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*p' +78 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&s'η +91 h 'η .tHand.close M&=/ +150 h / M&9& h &- M&% h %η .t\Forearm.seek :retract M&'\ +4 h '  ."MFBPCR"delay 3 seconds +86 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +214 ."MFBPCR"\ATV.dial :ASV F*5 n +51 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +35 h n ."MFBPCR"\ASV.dial :wasteR F*,  +43 h  ."MFBPCR"\AS.seek 0ml F*8'V +13 h ' .t\CC.seek :closed C'+(W +416 h (W C'& h &W .tHand.open M&A1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'7& h &W C'D% h % .t\CSV.dial CSR C'0  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '''[ +463 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'/& h &W C'J% h %4 .t\CSV.dial :flush C'F  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'i% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  'R  +37 h  +113  .t\CBV.dial :waste1 C'$ @ +76 h @ .t\CS.seek 0ml C'!'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'-& h &W C'Q% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'X'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +38 h @ C'[% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'a  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'K  +76 h  .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'U  +37 h  C'O% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Y'P +1116 h 'P .tdelay 5 seconds +304 h 'V ."MFBPCR"delay 1:00 +196 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +37 h  .t\CBV.dial :waste1 C'_ @ +76 h @ .t\CS.seek 0ml C'f'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C':  +38 h  C'4% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'k'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +709 h ' ."connect"\CSV.dial CTV C'G  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'N'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'j  +38 h  C'o% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '`'P +623 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +287 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'q  +38 h +75 .t\CTV.dial :air C'd  +150 h .t\CS.seek 0ml C'B'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C'v +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'x& h &W C'P% h %4 .t\CSV.dial :flush C'C  +38 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +37 h @ C'% h %p .t\CS.seek 7ml C'H'i +119 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +91 h 'i ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'z +38 h .t\CS.seek 0ml C''4 +506 h '4 C'|& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\\'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C'g'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'*& h &W C'% h %4 .t\CSV.dial :flush C'r  +37 h  .t\CS.seek 5ml C'm'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'1  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'~'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'n& h &W C'%% h %4 .t\CSV.dial :flush C'(  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'I  +37 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'E( +416 h ( M&5& h &ӷ M&2% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&A1 +150 h 1 M&)% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +91 h '\  #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&01 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +7 h (- M&e& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&(W +416 h (W C'R& h &W .tHand.open M&M1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'-& h &W C'Q% h %4 .t\CSV.dial CSR C'V  +37 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '^'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '[% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'X'[ +463 h '[ .t\CSV.dial :waste C'a +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'K'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'U% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'W'p +650 h 'p .tdelay 5 seconds +384 h 'V ."MFBPCR"delay 20 seconds +116 .t\CSV.dial :air C'Y @ +38 h @ C'h% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  +37 h  .t\CBV.dial :waste1 C': @ +76 h @ .t\CS.seek 0ml C'4'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'f& h &W C'k% h %4 .t\CSV.dial :flush C'G  +75 h  .t\CS.seek 5ml C't'p +476 ."MFBPCR"\ATV.dial :air3 F*6 % +107 h % F*j% h %V ."MFBPCR"\AS.seek 0.59ml F*o' +67 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C'8% h %p .t\CS.seek 7ml C'`'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'q  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'd'4 +766 h '4 #tDisinfecting external Intake with kill C'T& h &W C'B% h %4 .t\CSV.dial CSR C'v  +37 h  C']% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'x'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'P' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'C '  +38 h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'}  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ';'4 +607 h '4 .t\CSV.dial CSR C'y  +113 h  C'H% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'\ 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'z' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'|'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'l( +129 h '  ."MFBPCR"delay 3 seconds +287 h ( M&& h &ӷ M&L% h % .t\Forearm.seek :retract M&\\'\ +13 ."MFBPCR"\ATV.dial :ASV F** n +78 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +29 h n ."MFBPCR"\ASV.dial :wasteR F*r% h %  ."MFBPCR"\AS.seek 0.09ml F*g' +236 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&11 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun1 until completion of MFBPCR +9510 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*n I +86 h I F*%% h % ."MFBPCR"\AS.seek 0.12ml F*(' +625 h ' ."MFBPCR"\ATV.dial :air3 F*~ % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*=% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*" n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*+  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *s'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*p @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*9 I +86 h I ."MFBPCR"\AS.seek 0ml F*A'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*)'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*,  +43 h  #"MFBPCR"\Swish #1 of 2 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *0'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*e'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*/% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*7 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0102h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +65 h  ."MFBPCR"\ARV.dial :carrier F*&  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*E'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*^' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*a  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*U  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.536ml F*K'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +108 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*c n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*4  +43 h  ."MFBPCR"\ASV.dial :waste1 F*6  +64 h ."MFBPCR"\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refit!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0102h25ml.pcr F*q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*`  +21 h ."MFBPCR"\ATV.dial :air3 F*T % +87 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*x'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F**% h % ."MFBPCR"\AS.seek 0.81ml F*r'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*o% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0105h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*=% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *5'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*s I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*A n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.53ml F*D' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*)  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*&  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*J% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*M % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0105h25ml.pcr F*R% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*^'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *c'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*j % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*`'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*B% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*?  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F** n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*u' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*o % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +107 h  ."MFBPCR"delay 7 seconds +700 F*\\% h %< ."MFBPCR"\AS.seek 0.594ml F*m'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*n% h %\  ."MFBPCR"\AS.seek 0.6ml F*%'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*(  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*3  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*"% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*D  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*M'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*!'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0109h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*:'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*f n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*j  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*8  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*G' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*q  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*T % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*`'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0109h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*; @@T *z  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *?' +424 h ' ."MFBPCR"\ATV.dial :ASV F*> n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*F% h % ."MFBPCR"\AS.seek 0.62ml F**'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b * 'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*r'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*u % +43 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h % H ."MFBPCR"\AS.seek 0.735ml F*g' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*%% h % Ű ."MFBPCR"\AS.seek 0.72ml F*(' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*b% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*= n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*+% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*5% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*s' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*, n +86 h n ."MFBPCR"\ASV.dial :wasteR F*{  +21 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *D'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*)% h %V ."MFBPCR"\AS.seek 0.01ml F*e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*/' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h % q ."MFBPCR"\AS.seek 0.605ml F*''\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*&% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*E  +21 h  ."MFBPCR"\AS.seek 0ml F*J'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *R'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*M  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *Q' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*V n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b * '^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*$ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*['(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*- n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*a  +43 h  F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *i' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*U I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Y  +43 h  F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*c  +65 h  F*h% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *:'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*f n +65 h n F*W% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *k' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*6 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*_  +43 h  F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*4  +65 h  F*q% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *N'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*G n +64 h n F*T% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *B' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*w I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *`': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*v n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*d n +151 h n ."MFBPCR"\AS.seek 0ml F*x'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*P  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*C % +43 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +21 h F*}% h % ."MFBPCR"\AS.seek 0.96ml F*\ '0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*@% h % ."MFBPCR"\AS.seek 0.97ml F*'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*> I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *F'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *r' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*\\ n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*1% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *%'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*s % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*)% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*! % +108 h % ."MFBPCR"\AS.seek 0ml F*-'V +2547 h 'V #t\Asleep after completing 18tun1 assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@21:20:02, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun1 assays" #\Mission runtime was 2:25.606306 #-> exit #m\EXIT @1537039626.73CEST @1537114803.64CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage !o:Puck::Warning:bt[ "7/home/esp/esp2/utils/puckmoves.rb:223:in `giveup!'"2/home/esp/esp2/utils/puckmoves.rb:726:in `to'"6/home/esp/esp2/utils/puckmoves.rb:777:in `loadAt'"=/home/esp/esp2/utils/puckmoves.rb:898:in `loadFlushPuck'">/home/esp/esp2/protocol/utilities.rb:75:in `initialPurge'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"BRetaining claim on Arm because Hand is likely holding a puck! !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Wcollection microcontroller never signed on while reading Collection Clamp's status:@checkpoint: omitted #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ C' 2\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&E1 +150 h 1 M&F& h &M M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &H(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &K'η +91 h 'η .tHand.close M&L/ +150 h / M&M& h &- M&N% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'U& h &W .tHand.open M&V1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'Z% h %4 .t\CSV.dial CSR C'[  +151 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '^% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +462 h '[ .t\CSV.dial :waste C'` +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +2010 h '4 #t\Purging external Intake C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'g 'f  h +75 .t\CTV.dial :intake C'h  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'i'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'j  +75 h #t\Flushing upper manifold C'k& h &W C'l% h %4 .t\CSV.dial :flush C'm  +76 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'p% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'w& h &W C'x% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&\ & h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t-> exit #m\EXIT @1537114868.36CEST @1537115325.09CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> Puck.count A(D% h %X .t\SE.seek :down A(E'X +7 h 'X .t\SE.coast .Hand.open A(5 M&F1 +150 h 1 M&G% h %\  .t\SC.coast A(6 .t\SC.reconfigure SCtight .\SC.seek 1 + A($\ .(\ 1~b (H(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (I& h &( M&J& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &K( +22 h ( M&L& h & A(M& h &( .t\Forearm.seek 1 + 700 counts M&N' +89 h ' A(O& h &( .t\SE.seek :upperLimit A(P'Z +271 h 'Z A(Q% h %Z ."downElevator"\SE.seek :down A(R'X +100 (S% h % M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\SC.reconfigure SCtight .\SC.seek 2 +2 A(6 + ($\ .(\ 1~b (V( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (W& h &  M&X& h & .t\Elbow.seek 2 M&Y( +20 h ( M&Z& h & A([& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\\' +90 h ' A(]& h &  .t\SE.seek :upperLimit A(^'Z +270 h 'Z A(_% h %Z ."downElevator"\SE.seek :down A(`'X +100 (a% h % M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\SC.reconfigure SCtight .\SC.seek 3 +2 A(6 + ($\ .(\ 1~b (d( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (e& h & M&f& h & .t\Elbow.seek 3 M&g( +20 h ( M&h& h & A(i& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &j' +89 h ' A(k& h & .t\SE.seek :upperLimit A(l'Z +271 h 'Z A(m% h %Z ."downElevator"\SE.seek :down A(n'X +100 (o% h % M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +89 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\SC.reconfigure SCtight .\SC.seek 4 +2 A(6 + ($\ .(\ 1~b (r(,9 +201 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &,9 M&t& h & .t\Elbow.seek 4 M&u(w +20 h (w M&v& h &w A(w& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &x' +90 h ' A(y& h &,9 .t\SE.seek :upperLimit A(z'Z +270 h 'Z A({% h %Z ."downElevator"\SE.seek :down A(|'X +100 (}% h % M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\SC.reconfigure SCtight .\SC.seek 5 + A(6 +2 ($\ .(\ 1~b ((; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (& h &; M&& h &w .t\Elbow.seek 5 M&(M +19 h (M M&& h &M A(& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &'x +90 h 'x A(& h &; .t\SE.seek :upperLimit A( 'Z +270 h 'Z A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M& % h %x .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\SC.reconfigure SCtight .\SC.seek 6 +2 A(6 + ($\ .(\ 1~b ((K +200 h (K .t\SC.reconfigure SCloose A($.(\ 1~b (& h &K M&& h &M .t\Elbow.seek 6 M&(_ +19 h (_ M&& h &_ A(& h &K .t\Forearm.reconfigure TightForearm .\Forearm.seek 6 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &K .t\SE.seek :upperLimit A('Z +270 h 'Z A(% h %Z ."downElevator"\SE.seek :down A('X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\SC.reconfigure SCtight .\SC.seek 7 +2 A(6 + ($\ .(\ 1~b (([n +201 h ([n .t\SC.reconfigure SCloose A($.(\ 1~b ((& h &[n M&*& h &_ .t\Elbow.seek 7 M&+(C +20 h (C M&,& h &C A(& h &[n .t\Forearm.reconfigure TightForearm .\Forearm.seek 7 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(%& h &[n .t\SE.seek :upperLimit A(''Z +270 h 'Z A(/% h %Z ."downElevator"\SE.seek :down A(0'X +100 (1% h % M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #-> exit #m\EXIT @1537115371.57CEST @1537966262.48CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ C' 2\ S)&\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> finalPurge #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&N1 +150 h 1 M&O& h &M M&P% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &Q(- +165 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &T'η +91 h 'η .tHand.close M&U/ +150 h / M&V& h &- M&W% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'^& h &W .tHand.open M&_1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'b& h &W C'c% h %4 .t\CSV.dial CSR C'd  +150 h  C'e% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'f'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'g% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'h'[ +463 h '[ .t\CSV.dial :waste C'i +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'k& h &W C'l% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +38 h @ C'p% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'r  's  +37 h  +113  .t\CBV.dial :waste1 C't @ +38 h @ .t\CS.seek 0ml C'u'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'v& h &W C'w% h %4 .t\CSV.dial :flush C'x  +75 h  .t\CS.seek 5ml C'y'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +38 h @ C'{% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'}  +38 h  .t\CBV.dial :waste2b C'~  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'% h %4 .t\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'\ ' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &+'η +91 h 'η .tHand.open M&,1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t-> InitialPurge !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"(uninitialized constant InitialPurge: name:InitialPurge #-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'%& h & .tHand.open M&'1 +2 h 1 M&/& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &0(- +6 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.seek :garage M&3'η +91 h 'η .tHand.close M&4/ +150 h / M&5& h &- M&6% h %η .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +264 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C'<(W +416 h (W C'=& h &W .tHand.open M&>1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'B% h %4 .t\CSV.dial CSR C'C  +113 h  C'D% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'E'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'F% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'G +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'I'4 +2010 h '4 #t\Purging external Intake C'H& h &W C'K% h %4 .t\CSV.dial :flush C'L  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N +76 h .t\CTV.dial :intake C'O  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'P'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'R  +76 h #t\Flushing upper manifold C'S& h &W C'&% h %4 .t\CSV.dial :flush C'U  +75 h  .t\CS.seek 5ml C'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C')% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +506 h '0 .t\CSV.dial :waste C'X +38 h .t\CS.seek 0ml C'.'4 +507 h '4 C'^& h &W C'_% h %4 .t\CSV.dial :flush C'`  +37 h  .t\CS.seek 3ml C'b'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +37 h @ C'd% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'g +76 h .t\CS.seek 0ml C'f'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +7 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.seek CC M&m' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'o( +416 h ( M&p& h &ӷ M&n% h % .t\Forearm.seek :retract M&r'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &s(- +265 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &v'η +91 h 'η .tHand.open M&w1 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t-> exit #m\EXIT @1537966659.36CEST @1537970643.63CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiP!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> execute 18tundeployWCR.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"}compile error\ (ESP):3: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeployWCR.rb\ ^ #-> execute "18tundeployWCR.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"/home/esp/esp2/mission/18tundeployWCR.rb:55: syntax error, unexpected ')', expecting ']'\ standAloneWCR([nil, $wcrVol, $bac) {hotBleach}\ ^ #-> execute 18tundeployWCR !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):5: syntax error, unexpected tIDENTIFIER, expecting $end #-> exit #m\EXIT @1537970754.31CEST @1537970822.13CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 01:02:30CET01-Jan-70 .delayUntil "01:02:30.00" +14999 #-> execute 18tundeployWCR !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):1: syntax error, unexpected tIDENTIFIER, expecting $end #-> execute 18tundeployWCR.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"}compile error\ (ESP):2: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeployWCR.rb\ ^ #-> exit #m\EXIT @1537970903.89CEST @1537970991.92CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 01:02:30CET01-Jan-70 .delayUntil "01:02:30.00" +14999 #-> execute 18tundeployWCR !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):1: syntax error, unexpected tIDENTIFIER, expecting $end #-> execute 18tundeploy.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"|compile error\ (ESP):2: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeploy.rb\ ^ #-> execute 18tun1.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"rcompile error\ (ESP):3: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tun1.rb\ ^ #-> execute "18tun1" .GoodNews.suppressMail "Starting 18tun1 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:10:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun1 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:02 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tun1.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:11:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:02" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& h & S)#.0X exit #m\EXIT @1537971084.46CEST @1537971105.38CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeployWCR" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"/home/esp/esp2/mission/18tundeployWCR.rb:55: syntax error, unexpected ')', expecting ']'\ standAloneWCR([nil, $wcrVol, $bac) {hotBleach}\ ^ #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:11:54, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM #"shutdown"\Shutdown #t\Asleep til 6AM #\Continuing at 06:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: WCRPhase\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:13:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" +1799999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % .t\SS.configure SSconfig F*#,,'T(^20( b C'& S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:13:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase WCRPhase" #\Phase [WCRPhase] !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"$uninitialized constant WCRPhase: name: WCRPhase #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:13:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM ."shutdown"\SS.coast #t\Asleep til 6AM S)5 ."shutdown"\CC.coast #t\Continuing at 06:00 C'6 ."shutdown"\CS.coast E ;3\\0\\0 ."shutdown"\PC.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast .tGoodNews.suppressMail "Then, in tube 5: WCRPhase\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:15:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" ."shutdown"\SC.coast .\SE.coast A(6 !to:Delay::Late:bt["-/home/esp/esp2/lib/delay.rb:327:in `til'"2/home/esp/esp2/lib/delay.rb:450:in `delayTil'"3/home/esp/esp2/utils/sleep.rb:97:in `__send__'"0/home/esp/esp2/utils/sleep.rb:97:in `until'"//home/esp/esp2/utils/sleep.rb:106:in `til'"5/home/esp/esp2/utils/commskel.rb:159:in `napTil'"//home/esp/esp2/utils/mission.rb:46:in `at'"1/home/esp/esp2/mission/18tundeployWCR.rb:113"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"1/home/esp/esp2/mission/18tundeployWCR.rb:110"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):3:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"running 17 seconds late E ;3\\0\\0 + C'5 A(5 P%6 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )/ h  C' '0 h  P% %1 h  F* *2 h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *3  h  .t\SPE.configure SPEconfig F*\ 2 *4% h %&K .t\AS.configure ASconfig S)5% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'6& S)#.0X  A(#QK-0-\ b h  .t\CH.configure CHconfig .\PH.coast C'\ 2 P%?  h  .t\PH.configure PHconfig P%\ 2 +30 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +7 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'@% P%A% M&B% h %p %q ."rdySampler"Intake.select :close ."rdyProcessing"\PSV.dial :air h %= ."rdySampler"\Exhaust.select :close P%C  ."rdySampler"\CTV.dial :air M&D% C'E  h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &F'\ h ."rdySampler"\CSV.dial :waste1 C'G h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)H h  S)I  h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b 'J'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:15:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase WCRPhase" #\Phase [WCRPhase] !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"$uninitialized constant WCRPhase: name: WCRPhase #-> exit #m\EXIT @1537971340.36CEST @1537971459.29CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 06:00:31CET01-Jan-70 .delayUntil "06:00:31.00" +1803099 #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:17:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM #"shutdown"\Shutdown #t\Asleep til 6AM #\Continuing at 06:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: WCRPhase2\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:18:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" !o:Delay::Late:bt["-/home/esp/esp2/lib/delay.rb:327:in `til'"2/home/esp/esp2/lib/delay.rb:450:in `delayTil'"3/home/esp/esp2/utils/sleep.rb:97:in `__send__'"0/home/esp/esp2/utils/sleep.rb:97:in `until'"//home/esp/esp2/utils/sleep.rb:106:in `til'"5/home/esp/esp2/utils/commskel.rb:159:in `napTil'"//home/esp/esp2/utils/mission.rb:46:in `at'"1/home/esp/esp2/mission/18tundeployWCR.rb:113"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"1/home/esp/esp2/mission/18tundeployWCR.rb:110"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"running 31 seconds late E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ C' 2\ P% 0\ S)&\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:18:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase WCRPhase2" #\Phase [WCRPhase2] !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"%uninitialized constant WCRPhase2: name:WCRPhase2 #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:18:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM ."shutdown"\SS.coast #t\Asleep til 6AM ."shutdown"\CC.coast S)5 C'6 ."shutdown"\CS.coast #t\Continuing at 06:00 ."shutdown"\PC.coast E ;3\\0\\0 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast .\SC.coast .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:19:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" A(6 ."shutdown"\SE.coast !to:Delay::Late:bt["-/home/esp/esp2/lib/delay.rb:327:in `til'"2/home/esp/esp2/lib/delay.rb:450:in `delayTil'"3/home/esp/esp2/utils/sleep.rb:97:in `__send__'"0/home/esp/esp2/utils/sleep.rb:97:in `until'"//home/esp/esp2/utils/sleep.rb:106:in `til'"5/home/esp/esp2/utils/commskel.rb:159:in `napTil'"//home/esp/esp2/utils/mission.rb:46:in `at'"1/home/esp/esp2/mission/18tundeployWCR.rb:113"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"1/home/esp/esp2/mission/18tundeployWCR.rb:110"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):2:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"running 48 seconds late E ;3\\0\\0 + P%6 C'5 A(5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )/ h  C' '0 h  P% %1 h  F* *2 h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *3  h  .t\SPE.configure SPEconfig F*\ 2 *4% h %&K .t\AS.configure ASconfig S)5% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'6& h & .t\CC.configure CCconfig S)#.0X  A(#QK-0-\ b h  .t\CH.configure CHconfig .\PH.coast C'\ 2 P%?  h  .t\PH.configure PHconfig P%\ 2 +30 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +7 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'@% h %p P%A% ."rdySampler"Intake.select :close h %q M&B% ."rdySampler"\Exhaust.select :close ."rdyProcessing"\PSV.dial :air ."rdySampler"\CTV.dial :air h %= C'D  P%C  M&E% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &F'\ h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %G' h ."rdySampler"\CSV.dial :waste1 C'H h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)I h  S)J  h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'K'4 S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:19:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C']& h & .tHand.open M&^1 +150 h 1 M&_& h &M M&`% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &a(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &d'η +90 h 'η .tHand.close M&e/ +150 h / M&f& h &- M&g% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +265 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C'n& h &W .tHand.open M&o1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'r& h &W C's% h %4 .t\CSV.dial CSR C't  +150 h  C'u% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'v'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'w% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'x'[ +463 h '[ .t\CSV.dial :waste C'y +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'z'4 +2010 h '4 #t\Purging external Intake C'{& h &W C'|% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'\ % h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&"& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&,/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&%% h % .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &2'η +91 h 'η .tHand.open M&31 +150 h 1 M&4% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 16:19:16CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:19:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*6% !to:Storage::Error:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Out of Pucks h %&K #"readyMFB"Readying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* #t-> exit +6 #m\EXIT @1537971572.56CEST @1537971724.03CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi$; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute 18tundeployWCR !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):1: syntax error, unexpected tIDENTIFIER, expecting $end #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:22:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM #"shutdown"\Shutdown #t\Asleep til 6AM #\Continuing at 06:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:22:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" +1799999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ S)&\ P% *\ F*\ 9\ C' 2\ .t\SPE.coast P% 0\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& h & S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:22:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R 'Q  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 16:22:51CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:22:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% h %&K !to:Storage::Error:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Out of Pucks #"readyMFB"Readying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* #t-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:22:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM ."shutdown"\SS.coast S)5 #t\Asleep til 6AM ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast #t\Continuing at 06:00 ."shutdown"\Forearm.coast P%6 E ;3\\0\\0 M&5 ."shutdown"\Elbow.coast .tGoodNews.suppressMail "Then, in tube 5: WCRPhase\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:23:37, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" ."shutdown"\SC.coast A(6 ."shutdown"\SE.coast !to:Delay::Late:bt["-/home/esp/esp2/lib/delay.rb:327:in `til'"2/home/esp/esp2/lib/delay.rb:450:in `delayTil'"3/home/esp/esp2/utils/sleep.rb:97:in `__send__'"0/home/esp/esp2/utils/sleep.rb:97:in `until'"//home/esp/esp2/utils/sleep.rb:106:in `til'"5/home/esp/esp2/utils/commskel.rb:159:in `napTil'"//home/esp/esp2/utils/mission.rb:46:in `at'"1/home/esp/esp2/mission/18tundeployWCR.rb:113"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"1/home/esp/esp2/mission/18tundeployWCR.rb:110"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):3:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"running 3:22 late E ;3\\0\\0 +2 C'5 A(5 M&6 +4 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % +30 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .tGate.power :core,:ON .Gate.power :raw,:ON +99 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.reconfigure ASconfig F*#,,'T(^20( b ."readyMFB"\AS.home.seek :home F*#,,'T(^20( b *' +21 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.coast F*5 .t\AS.configure ASconfig h ' !"readyMFB"o:Slide::Error :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:@axisName"Analytical Syringe: mesg"*Analytical Syringe aborted at 0.497ml: @replyo:I2C::Servo::AbsMove3Reply : @channeli: @statusIS:I2C::Servo::Status: enabledF: pastRLSF: pastFLSF:pastThresholdF: lostF: homeF: positioni: velocityi: currenti:pwmi:erri: inPressi: outPressi:deltaPressi: voltagef12.162036327933235yo: @type2F: @error: aborted: @tagi: @sourcei/:@destinationi%:@timestampu: Time %s :@checkpoint: omitted S) % F*#,,'T(^20( b ."readyMFB"BadNews.suppressMail "Analytical Syringe aborted at 0.497ml",Subject: "Slide::Error in readyMFB" #Waiting to Resume from Checkpoint "Analytical Syringe aborted at 0.497ml" h % .t\SS.configure SSconfig C'\ & S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:23:43, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase WCRPhase" #\Phase [WCRPhase] !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"$uninitialized constant WCRPhase: name: WCRPhase #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:23:43, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast #t\Asleep til 6AM C'6 ."shutdown"\CS.coast .\PC.coast #t\Continuing at 06:00 ."shutdown"\Forearm.coast E ;3\\0\\0 M&5 ."shutdown"\Elbow.coast .\SC.coast A(6 ."shutdown"\SE.coast .tGoodNews.suppressMail "Then, in tube 5: initialPurge\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:26:42, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" !o:Delay::Late:bt["-/home/esp/esp2/lib/delay.rb:327:in `til'"2/home/esp/esp2/lib/delay.rb:450:in `delayTil'"3/home/esp/esp2/utils/sleep.rb:97:in `__send__'"0/home/esp/esp2/utils/sleep.rb:97:in `until'"//home/esp/esp2/utils/sleep.rb:106:in `til'"5/home/esp/esp2/utils/commskel.rb:159:in `napTil'"//home/esp/esp2/utils/mission.rb:46:in `at'"1/home/esp/esp2/mission/18tundeployWCR.rb:113"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"1/home/esp/esp2/mission/18tundeployWCR.rb:110"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):4:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"running 3:37 late E ;3\\0\\0 +2 A(5 C'5 P%6 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )) h  C' '6 h  P% %< h  F* *= h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ F*\ 9\ C' 2\ .t\SPE.coast P% 0\ F*\ 9\ *\ 9\ *3  h  .t\SPE.configure SPEconfig F*\ 2 *:% h %u .t\AS.configure ASconfig F*#,,'T(^20( b S).% h % .t\SS.configure SSconfig C'@& S)#.0X% h %X .t\SE.configure SEconfig .\CH.coast A(#QK-0-\ b C'  h  .t\CH.configure CHconfig C'\ 2 .t\PH.coast P%I  h  .t\PH.configure PHconfig P%\ 2 +30 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +7 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'H% h %p P%K% ."rdySampler"Intake.select :close .\Exhaust.select :close M&J% ."rdySampler"\CTV.dial :air h %q ."rdyProcessing"\PSV.dial :air h %= P%N  C'M  M&O% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &L'\ h ."rdySampler"\CSV.dial :waste1 C'Q h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)R h  S)S  h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b 'P'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:26:47, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase initialPurge" #\Phase [initialPurge] #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&e1 +150 h 1 M&h& h &M M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &k(- +166 h (- M&j& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &o'η +91 h 'η .tHand.close M&p/ +150 h / M&r& h &- M&;% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +264 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'y& h &W .tHand.open M&n1 +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'v& h &W C'~% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\ ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'" +37 h .t\CS.seek 0ml C''4 +507 h '4 C'(& h &W C'z% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C'$'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'}% h %0 .t\CS.seek 5ml C'#'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'!'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +507 h '0 .t\CSV.dial :waste C'- +75 h .t\CS.seek 0ml C'+'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&%& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&&' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&0% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &)(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&31 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  !to:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message@undefined local variable or method `wcrVol' for main:Object: name: wcrVol #-> execute "18tundeployWCR" .GoodNews.suppressMail "Starting 18tundeployWCR w/pucks in tube 5\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:26:47, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployWCR begun" #\Preparing to sleep until 6AM ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast #t\Asleep til 6AM C'6 ."shutdown"\CS.coast #t\Continuing at 06:00 ."shutdown"\PC.coast P%6 E ;3\\0\\0 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast .\SC.coast .tGoodNews.suppressMail "Then, in tube 5: initialPurge\\n/home/esp/esp2/mission/18tundeployWCR.rb:113\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:27:40, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 06:00" ."shutdown"\SE.coast A(6 !to:Delay::Late:bt["-/home/esp/esp2/lib/delay.rb:327:in `til'"2/home/esp/esp2/lib/delay.rb:450:in `delayTil'"3/home/esp/esp2/utils/sleep.rb:97:in `__send__'"0/home/esp/esp2/utils/sleep.rb:97:in `until'"//home/esp/esp2/utils/sleep.rb:106:in `til'"5/home/esp/esp2/utils/commskel.rb:159:in `napTil'"//home/esp/esp2/utils/mission.rb:46:in `at'"1/home/esp/esp2/mission/18tundeployWCR.rb:113"//home/esp/esp2/utils/mission.rb:19:in `[]'"4/home/esp/esp2/utils/mission.rb:19:in `mission'"1/home/esp/esp2/mission/18tundeployWCR.rb:110"1/home/esp/esp2/lib/framework.rb:56:in `load'"1/home/esp/esp2/lib/framework.rb:56:in `load'"5/home/esp/esp2/lib/framework.rb:107:in `execute'"(ESP):5:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"running 6:58 late E ;3\\0\\0 +2 M&6 A(5 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )@ h  C' 'A h  P% %B h  F* *D h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ F*\ 9\ .t\SPE.coast C' 2\ P% 0\ F*\ 9\ *\ 9\ *E  h  .t\SPE.configure SPEconfig F*\ 2 *F% h %&K .t\AS.configure ASconfig F*#,,'T(^20( b S)G% h % .t\SS.configure SSconfig C'>& h & S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\\nCTD data unavailable\\nCan@16:27:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployWCR phase initialPurge" #\Phase [initialPurge] #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\\& h & .tHand.open M&p1 +150 h 1 M&r& h &M M&;% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &_(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &u'η +91 h 'η .tHand.close M&k/ +150 h / M&C& h &- M&a% h %η .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&q1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2009 h '4 #t\Purging external Intake C' & h &W C'\ % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'w +37 h .t\CS.seek 0ml C'}'4 +507 h '4 C'$& h &W C'#% h %4 .t\CSV.dial :flush C'!  +37 h  .t\CS.seek 3ml C'-'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'%% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'5'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'? +76 h .t\CS.seek 0ml C'|'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M& exit #m\EXIT @1537972172.27CEST @1537972356.78CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi\\!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeployv2" .GoodNews.suppressMail "Starting 18tundeployv2 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:32:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployv2 begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:32:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ C' 2\ P% 0\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:33:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:33:09CEST26-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:33:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %8 .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*I  +11 h  ."readyMFB"\ASV.dial :waste1 F*H  h +912 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'K% h %4 S)J% h % S)M% h % .tIntake.select :close S)0 +6 )N% h % .t\SSV.dial :exhaust S)O  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9Td +100 C'99 h 9Xd +100 C'I9 h 9(\\d +100 C'H9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'G9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 & h &ӷ &( .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit .t\Elbow.seek CC M&$&.,(\ #b A(I'Z M&H(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(G& h &( A(R& h &( M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +142 h ( M&W& h & A(S& h &( .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 1 M&X'C +93 h 'C .t\Elbow.hold M&T, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %C A(]& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&\\'o +20 h 'o M&^& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +23 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ +2 A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +18 h ` .t\AIV.dial :bypass C'e { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P%| +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'\  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A()& h &  .t\SE.seek 19.71 Pucks below Top Plate A( ' +66 h ' A(&& h &  M&8& h & .t\Elbow.seek 2 M&=( +6 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&:& A(B'X h & M&D% h %8 .t\Forearm.seek :retract M&E'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&F(ӷ +143 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +151 h  .t\PS.seek :empty P%Y' +2609 h ' .t\CBV.dial :waste1 C'] @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +37 h @ .t\CS.seek 10ml C'c' +2648 h ' .t\CSV.dial CTV C'Z  +37 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +151 h C'I& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h &( M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +143 h ( M&o& h & A(b& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &A' +89 h ' A(6& h &( .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +264 h '\\ M&r& h & A(C& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'C +26 h 'C .t\Elbow.hold M&a, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(j- h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %C A(p& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&u'o +20 h 'o M&O& h & A(t& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +415 h ( A(& h &( A( & h &( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +143 h ( M& & h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(q& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%,' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M&)& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&:% h %η .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &9' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C' & h &W .tHand.open M&B1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1094 h ' .t\PSV.dial :air P%g  +38 h  .t\PS.seek 0ml P%G' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +76 h .tdelay 2 seconds +200 P%H% h %r .t\PS.seek 0.475ml P%[' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +75 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +76 h @ .t\PS.seek 0ml P%Q' +2609 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +75 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +7 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'6 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %F' +1313 h ' .t\PSV.dial :air P%"  +37 h  .t\PS.seek 2.5ml P%r' +2610 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& ."MFBPCR"\ASV.dial :SPEdelivery F*O @@T h &W F*t  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +42 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%s' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +112 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%,' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %)'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%'  +112 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%: +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*@  +43 h  F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *D'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F* % h %Q ."MFBPCR"\AS.seek 0.01ml F*B' +171 h ' .t\PSV.dial :mixloop P%= +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*9'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*>% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  'M  +37 h  +113  .t\CBV.dial :waste1 C'H @ +38 h @ .t\CS.seek 0ml C'S'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'I' +1626 h ' ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +7 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&6/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&F% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (A( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +90 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A( 'X M&& h & M&q% h %8 ."WCR"\Forearm.seek :retract M&{'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +6 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +21 h  ."MFBPCR"\AS.seek 0.3ml F*+': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*%'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*R % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*U  +87 h F*G% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *M'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*H I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*W'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*[ n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*L  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *]% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *g' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *S% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *l' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*_% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *^' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*Q% h % ."MFBPCR"\AS.seek 0.279ml F*\\'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Z n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*f % +43 h % F*I% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *i'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*m I +21 h I F*V% h %v ."MFBPCR"\AS.seek 0.059ml F*o'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*6 % +21 h % F*5% h %H ."MFBPCR"\AS.seek 0.069ml F*E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*"% h % ."MFBPCR"\AS.seek 0.769ml F*F' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h % q ."MFBPCR"\AS.seek 0.794ml F*p' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*C I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *`' +4416 h ' ."MFBPCR"\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.seek :empty F*'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*t% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*j  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*; % +108 h % F*y% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*w n +108 h n ."MFBPCR"\ASV.dial :wasteR F*}% h %  ."MFBPCR"\AS.seek 0.09ml F*?' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*{' +625 h ' ."MFBPCR"\ATV.dial :air3 F*b % +21 h % F*c% h % ."MFBPCR"\AS.seek 0.13ml F*v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*~% h % ."MFBPCR"\AS.seek 0.23ml F*Y' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*A% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *1'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +85 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*4'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F**% h %(A ."MFBPCR"\AS.seek 0.6ml F*2'\  +1031 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*@% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *8' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*: % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*\  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *9'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*X' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*H % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +22 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226038 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +94 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'E ` +19 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'x  'F  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +95 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +76 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +151 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&& h & A($& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +253 h 'K M&/& h & A(1& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'C +26 h 'C ."WCR"\Elbow.hold M&,, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(7- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&)% h %C A(4& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&0'o +20 h 'o M&3& h & A(*& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(<' +36 h ' M&2& h & M&@% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&:(ӷ +143 h (ӷ M&8& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'D( +416 h ( A(& h &( A(&& h &( M&(& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &B( +142 h ( M&& h & A(>& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C ."WCR"\Elbow.hold M&+, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&9% h %C A(U& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&G'o +20 h 'o M&%& h & A(H& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 +2 A(6 h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +265 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +415 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'F& h &W C'x% h % .t\CSV.dial CSR C'h  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +37 h  .t\CBV.dial :waste2b C'q  +76 h  .t\CS.seek 0ml C'w'4 +765 h '4 #t\Flushing upper manifold C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ 'c  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'Y'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C') +75 h .t\CS.seek 0ml C'4'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'*% h %4 .t\CSV.dial CSR C'2  +151 h  C'@% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'A' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'8' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'\  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'&'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'(  +37 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '>' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C':  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'9  +38 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N 'X  +38 h +75 .t\CTV.dial :air C'B  +151 h .t\CS.seek 0ml C'L'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +507 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'f'4 +506 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +38 h  .t\CS.seek 3ml C'\\'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'F% h %4 .t\CSV.dial :flush C'x  +37 h  .t\CS.seek 5ml C'h'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'd  +38 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +37 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  ' +37 h +76 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +112 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1373 h ' .t\CSV.dial CBV C'? +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&*& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &2(- +7 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&A% h %η .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C',(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'J% h %4 .t\CSV.dial CSR C'&  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 ':% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '>'[ +463 h '[ .t\CSV.dial :waste C'9 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2010 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'X  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B +75 h .t\CTV.dial :intake C'H  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'L'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'<% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +506 h '0 .t\CSV.dial :waste C'[ +38 h .t\CS.seek 0ml C'+'4 +506 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'm +75 h .t\CS.seek 0ml C'.'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&D& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +6 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +265 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +90 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Preparing to sleep until 14:00 29-sep-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00 29-sep-18 #\Continuing at 14:00CEST29-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:34:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST29-Sep-18" +263638 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi\\!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *&'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*:  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*9' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*>  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*X  +108 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +22 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*^  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*D'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0108h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*6% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*/ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +107 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +22 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0108h25ml.pcr F*2% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*\  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*:'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*9  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +31 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0111h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*D  +10 h  F*8% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*d I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*| n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +21 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0111h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*{% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +32 h  ."MFBPCR"\ATV.dial :air3 F*4 % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*l I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*s % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +22 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +22 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*x @@T ."MFBPCR"\ASV.dial :SPEdelivery F*=  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*,  +151 h F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :ASV F*E n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*"% h % ."MFBPCR"\AS.seek 0.62ml F*d'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *|'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*I'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *n' +424 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +21 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*u' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*j' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*; n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*q% h % zG ."MFBPCR"\AS.seek 0.365ml F*y' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*c% h % ."MFBPCR"\AS.seek 0.579ml F*w' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*6% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*o'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*{ % +108 h % F*~% h %V ."MFBPCR"\AS.seek 0.01ml F*1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*$% h % ."MFBPCR"\AS.seek 0.58ml F**' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h % q ."MFBPCR"\AS.seek 0.605ml F*!'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*A% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*k  +21 h  ."MFBPCR"\AS.seek 0ml F*Y'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *2'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*'  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *\ ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*v n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*J  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*( n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*0  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*R  +42 h  F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *>': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*U  +64 h  F*9% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *%'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*N n +65 h n F*H% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *B' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*S I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*M  +43 h  F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *G': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*_  +64 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *Z'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*< n +65 h n F*^% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*Q  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*f n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *P'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*+ n +150 h n ."MFBPCR"\AS.seek 0ml F*e'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*i  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*W % +43 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*m% h % ."MFBPCR"\AS.seek 0.96ml F*D'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*C% h % ."MFBPCR"\AS.seek 0.97ml F*h'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*E I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *"'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *`'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *b'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*|  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *I' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F*n' +9880 h ' ."MFBPCR"\ATV.dial :waste F*p n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*a% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*u % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*c  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *V'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*o% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*~% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*1'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*4  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*J n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*('V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538222400.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )0 h  C' '& h  P% % h  F* *  h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)R% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'X& S)#.0X% P%#A d`xb h %\  .t\Forearm.configure LooseForearm M&#Q@2^d^2b &N& h &- .t\Elbow.configure LooseElbow A(H& M&$ &.,(#b h &( .t\SC.configure SCloose A($.(\ 1~b (%% h %X .t\SE.configure SEconfig .\CH.coast A(#QK-0-\ b C'S  h  .t\CH.configure CHconfig C'\ 2 .t\PH.coast P%B  h  .t\PH.configure PHconfig P%\ 2 +29 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +6 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'L% h %p P%M% ."rdySampler"Intake.select :close M&l% ."rdySampler"\Exhaust.select :close h %q ."rdySampler"\CTV.dial :air ."rdyProcessing"\PSV.dial :air h %= P%]  C'_  M&s% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &G'\ h ."rdySampler"\CSV.dial :waste1 C'< h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)^ h  S)Z  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M&/ .t\Elbow.jog 1500 M&Q* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b '['4 S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:35:50CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'C& h & .tHand.open M&1 +150 h 1 M&E& h &M M&h% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &`'η +91 h 'η .tHand.close M&d/ +150 h / M&|& h &- M&% h %η .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'a(W +416 h (W C'n& h &W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'K% h %4 .t\CSV.dial CSR C'j  +150 h  C';% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'q +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'r& h &W C'y% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '}  h +75 .t\CTV.dial :intake C'V  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'w  +75 h #t\Flushing upper manifold C'& h &W C'6% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'o% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ +113 h .t\CTV.dial :air .\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C'7 +37 h .t\CS.seek 0ml C'$'4 +507 h '4 C'& h &W C'@% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C'1'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'*% h %0 .t\CS.seek 5ml C'A'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'!'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'z'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'J( +416 h ( M&v& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&-1 +150 h 1 M&(% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:35:56CEST26-Sep-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:35:50CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*t% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'x& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&0% h %\  A(&% h %X A(. h ., A( & h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&R% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &X( +6 F*:% h %&K ."readyMFB"\ATV.dial :waste2 F*U % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *9' +160 h ( M&>& h & A(N& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +89 h ' A(%& h &  .t\SE.seek 17.71 Pucks below Top Plate A(S'* +87 h '* A(B& h &  M&L& h & .t\Elbow.seek 2 M&M( +6 h ( M&l& h & A(s& h &  .t\Forearm.seek 2 + 300 counts M&_'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &  +151 h  C'N% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '5' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +7 '%% h %  .t\CS.seek 4.5ml C'T'` +859 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'B  +38 h  .t\CS.seek 1.275ml C'L' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'l   's9 h 9d +100 C'9 h 9d +100 C'<9 h 91d +100 C']9 h 9J d +100 C'Z9 h 9bd +100 C'P9 h 9zd +100 C'X9 h 9d +100 C'H9 h 9d +100 C'Q9 h 9 d +100 C'F9 h 9$d +100 C'_9 h 9(d +100 C'S9 h 9 ,d +100 C'^9 h 9$0d +100 C'M9 h 9<4d +100 C'9 h 9S8d +100 C'J9 h 9kd +100 C'9 h 9Td +100 C'b9 h 9kd +100 C',9 h 9d +100 C'n9 h 9d +100 C' 9 h 9d +100 C'|9 h 9d +100 C'h9 h 9d +100 C'9 h 9d +100 C'K9 h 9 d +100 C'j9 h 9 d +100 C'I9 h 96d +100 C'p9 h 9Ld +100 C'm9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'q9 h 9d +100 C';9 h 9d +100 C' 9 h 9d +100 C'y9 h 9d +100 C'?9 h 9d +100 C'}9 h 9d +100 C'r9 h 9(d +100 C'V9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'c9 h 9d +100 C'9 h 9d +100 C'69 h 9d +100 C'9 h 9d +100 C'o9 h 9d +100 C'w9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'{9 h 9 j d +100 C'79 h 9 $d +100 C'/9 h 9 (d +100 C'9 h 9 ,d +100 C'@9 h 9 0d +100 C'$9 h 9 4d +100 C'49 h 9 8d +100 C'*9 h 9 9 h 9 d +100 C'N9 h 9 d .t\CH.hold 5:00,85C C'U   P h  +30000  C'%9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'5'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'B @ +113 h @ .t\CSV.dial :air C'T @ +38 h @ .t\CS.seek 5ml C's'p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'< @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C']  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'Z @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'P'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'X +113 h .t\CBV.dial CSV C'H  +19 h C'Q% h %4 .t\CS.seek 5ml C'F'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'_  +37 h C'S% h %p .t\CS.seek 7ml C'^'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'M @ +38 h @ .t\CS.seek 10ml C'' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'J ` +132 h ` #tLysing Complete C'=& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&G% h %\  A(W% h %X A(e. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (E(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (\\& h &( M&z& h &ӷ M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &f( +143 h ( M&"& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &C' +89 h ' A( & h &( .t\SE.seek 1.71 Pucks below Top Plate A(g': +244 h ': M&d& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'C +26 h 'C .t\Elbow.hold M&9, +6 h , .t\Elbow.reconfigure TightElbow A(`- h -+: M&$&.,(\ #b .t\SE.seek 2.04 Pucks below Top Plate A(O' +27 h ' M&% h %C A(b& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &,'o +20 h 'o M&n& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &|' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(h' +37 h ' M&& h & M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +89 h '\  .t\Elbow.seek CC M&I(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'q( +415 h ( A(;& h &( A( & h &( M&y& h &ӷ M&?% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &r( +143 h ( M&V& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &c1 +150 h 1 M&% h %C A(6& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&o& h & A(w& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (% h %1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'/ @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'@ ` +19 h ` .t\AIV.dial :bypass C'$ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'4& h & .tHand.open M&*1 +2 h 1 M&1& h & M&A% h %\  .t\Elbow.seek FlushPuck::Garage M&~(- +166 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&Y'η +91 h 'η .tHand.close M&3/ +150 h / M&2& h &- M&\ % h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'#(W +416 h (W C'a& h &W .tHand.open M&)1 +150 h 1 M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%-% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%t  +38 h  .t\PS.seek 0ml P%(' +1597 h ' .t\PSV.dial PSR P%0 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%x' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%&  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%:  +37 h  .t\PS.seek 2.5ml P%R' +2610 h ' .t\PSV.dial :mixloop P%> +38 h .t\PS.seek 0ml P%N' +2609 h ' .t\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%%' +2610 h ' .t\PSV.dial :mixloop P%L +37 h .t\PS.seek 0ml P%B' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.seek CC M&]' +90 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C's( +416 h ( M&X& h &ӷ M&H% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &P(- +265 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &F'η +91 h 'η .tHand.open M&M1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'J& h & M&=% h %\  A(G% h %X A(W. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (e( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (\\& h &  M&z& h &- M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &"( +166 h ( M&& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(& h &  .t\SE.seek 16.71 Pucks below Top Plate A(d'; +96 h '; A(& h &  M&`& h & .t\Elbow.seek 2 M&( +6 h ( M&b& h & A(n& h &  .t\Forearm.seek 2 + 300 counts M& '8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&C& A(K'X h & M&f% h %8 .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'O(W +416 h (W .tHand.open M&|1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'j  +150 h  .t\CSV.dial CTV C'8  +19 h  .t\CS.seek 5ml C';'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C' ' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'y'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%?  +37 h  .t\PS.seek 2.5ml P%V' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%I +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%c  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C'6 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'o  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'w @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'} ` +19 h ` .t\CSV.dial :air C'r @ +37 h @ .t\CS.seek 10ml C'' +2648 h ' .t\CSV.dial CTV C'{  +37 h  .t\CS.seek 0ml C'/'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'@  +151 h C'$& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&4% h %\  A(*% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (A(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&h& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +143 h ( M&& h & A(E& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &( .t\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') M&3& h & A(2& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &\ 'C +25 h 'C .t\Elbow.hold M&7, +7 h , .t\Elbow.reconfigure TightElbow A(Y- h -+) M&$&.,(\ #b .t\SE.seek 3.04 Pucks below Top Plate A(u' +27 h ' M&v% h %C A(~& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M& 'o +21 h 'o M&q& h & A(a& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &)' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(!' +36 h ' M&& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&t(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&(' +91 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h &( A(& h &( M&x& h &ӷ M&:% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&R& h & A(U& h &( .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 1 M&N'C +93 h 'C .t\Elbow.hold M&L, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &%1 +150 h 1 M&T% h %C A(& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &<'o +21 h 'o M&Z& h & A('& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &.' +22 h ' A(#% h % ."downElevator"\SE.seek :down A(X'X +100 (H% h %  M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%S  +2 A(6 +111 h  P%5% h % .t\PS.seek 0.499ml P%M' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%l +38 h .t\PRV.dial :air P%Q  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%J  +38 h .t\CBV.dial AIV C'=  +38 h .t\PRV.dial between CBV and air P%G  +18 h  .t\PRV.dial :air P%W  +19 h  P%\\% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +251 h ' .t\PRV.dial :waste2 P%i @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %F' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&& h & M&P% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&^/ +150 h / M&& h &- M&C% h %η .t\Forearm.seek :retract M&f'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +265 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +91 h ' .t\CC.seek :closed C'd(W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  C's& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%| @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%9  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%j  +76 h  .tdelay 2 seconds +200 P%8% h % .t\PS.seek 1.499ml P%,' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%e' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%;' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%?  +37 h  .tdelay 2 seconds +200 P%B% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%I% h %r .t\PS.seek 0.475ml P%c' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%V  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%6'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%o  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%w  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%} +75 h .t\PTV.dial :PRV,avoiding: :puck P%r @ +75 h @ .t\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial :air P%{  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%@' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%$  +75 h  .t\PS.seek 2.5ml P%4' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%* +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%1  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %E'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1312 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :PML P%2 +37 h .t\PS.seek 0ml P%Y' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'v& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'~& h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*q @@T .t\AIV.dial :bypass F*a  .t\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%-% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*k I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *\ '6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%0' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%u' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%) +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%7' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*&  +22 h ' .t\PSV.dial PSR P% +21 h  F*O% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%x' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*: % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +38 h  .t\PS.seek 0ml P%(' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*t  +22 h  F*%% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *T' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Z' +217 ."MFBPCR"\ATV.dial :AIV F*' I +43 h I F*#% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%> +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%H' +2610 h ' .t\PSV.dial PSR P%] +113 h P%N% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%S' +739 h ' ."MFBPCR"delay 5 seconds +500 F*59 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*. n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X% h % ."MFBPCR"\AS.seek 0.041ml F*A'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%l' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%Q +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +2609 h ' .t\PSV.dial PSR P%= +113 h P%G% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%W' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\\  +37 h  .t\PS.seek 0ml P%M' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%i +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%z' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%P  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %`' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*b I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *L'V +849 h ' .t\PSV.dial :air P%^  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %_' +5106 h ' .t\PSV.dial :air P%n  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %+' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*p  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *f'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F* % h %Q ."MFBPCR"\AS.seek 0.01ml F*' +170 h ' .t\PSV.dial :mixloop P%K +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %s' +618 h ' ."MFBPCR"\ATV.dial :waste F*| n +129 h n ."MFBPCR"\AS.seek :empty F*m'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*9  +86 h  F*j% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *8' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*F  +43 h F*,% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'e  +37 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'?'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'B' +1625 h ' ."connect"\CSV.dial CTV C';  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'I @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*V  +22 h ."MFBPCR"delay 3 seconds +300 F*c% h %_ ."MFBPCR"\AS.seek 0.434ml F*o'ݏ +110 h '4 .t\CSV.dial CSR C'w  +37 h  C'6% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'}'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*r  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*{'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*y n +107 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'$' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'*'4 +2647 h '4 C'1& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'E( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &3(- +264 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &a'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'k& h & M&% h %\  A(% h %X A([. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&0& h &- M&)% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &u( +166 h ( M&&& h & A(O& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A(\ 'L +107 h 'L A(7& h &  M&:& h & ."WCR"\Elbow.seek 2 M& ( +7 h ( M&U& h & A(t& h &  ."WCR"\Forearm.seek 2 + 300 counts M&%'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &x/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down M&& A(R'X h & M&'% h %8 ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.seek CC M&((ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&T& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +64 ."MFBPCR"\ATV.dial :waste2 F*] % +27 h ' ."WCR"\CC.seek :closed C'N(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +400 h (W ."WCR"Hand.open M&!1 +150 h 1 M&<% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*.  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *X' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'S& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'Q  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."MFBPCR"\ASV.dial :wasteR F*%% h %  ."MFBPCR"\AS.seek 0.09ml F*\ ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*]  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +85 h I F*R% h % ."MFBPCR"\AS.seek 0.12ml F*#' +626 h ' ."MFBPCR"\ATV.dial :air3 F*E % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*.% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h % ."MFBPCR"\AS.seek 0.255ml F*Z'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*= n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*G  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *W'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*l @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*A I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*i'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*P  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *b'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*^'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*z% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*n% h %\  ."MFBPCR"\AS.seek 0.5ml F*_'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*p% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *`' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*C % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep2915h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*K  +10 h  F*f% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*8  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*e' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*?  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*I % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*6'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*V n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 F*{  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226068 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'1 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'" @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'd  +95 h  C'% h % ."WCR"\CSV.dial between air and CTV C'g ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'v  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '~' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'2  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'-  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'[' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'k @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C')  +94 h  C'a% h % ."WCR"\CSV.dial between air and CTV C'0 ` +56 h ` ."WCR"\CS.seek 7.288ml C'O' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '&  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '7'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C':  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '3' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C't  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Y  +38 h  C'U% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'x' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'u @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C''  +94 h  C'>% h % ."WCR"\CSV.dial between air and CTV C'% ` +57 h ` ."WCR"\CS.seek 7.288ml C'T' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C']  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'R'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'E' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'#& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&<% h %\  A(.% h %X A(!. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (S(( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Q& h &( M&& h &ӷ M&H% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&Z& h & A(=& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &G' +89 h ' A(& h &( ."WCR"\SE.seek 3.71 Pucks below Top Plate A(\\' +224 h ' M&J& h & A(W& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &A'C +26 h 'C ."WCR"\Elbow.hold M&M, +6 h , ."WCR"\Elbow.reconfigure TightElbow A( - h -+ M&$&.,(\ #b ."WCR"\SE.seek 4.04 Pucks below Top Plate A(P' +27 h ' M&% h %C A(i& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&b& h & A(z& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &^' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(n' +37 h ' M&& h & M&p% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  ."WCR"\Elbow.seek CC M&C(ӷ +142 h (ӷ M&`& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&K/ +150 h / ."WCR"\CC.seek :open C'f( +416 h ( A(L& h &( A(& h &( M&X& h &ӷ M&|% h % ."WCR"\Forearm.seek :retract M&9'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&F& h & A(,& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &j'C +92 h 'C ."WCR"\Elbow.hold M&8, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&m% h %C A(?& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&;'o +21 h 'o M&e& h & A(I& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &B' +22 h ' A(/% h % ."downElevator"\SE.seek :down A(c'X +100 (w% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +90 h '\  +21 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&61 +2 A(6 h 1 M&& h & M&r% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&@/ +150 h / M&y& h &- M&}% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'd& h &W .tHand.open M&1 +150 h 1 M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'v& h &W C'% h % .t\CSV.dial CSR C'h  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'4'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '-'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'[& h &W C'k% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ')'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'0% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'&  +37 h  +113  .t\CBV.dial :waste1 C'O @ +76 h @ .t\CS.seek 0ml C':'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'7& h &W C't% h %4 .t\CSV.dial :flush C'Y  +76 h  .t\CS.seek 5ml C'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +38 h @ C'x% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C''  +76 h  .t\CS.seek 0ml C'>'4 +766 h '4 #t\Flushing upper manifold C'%& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C']'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'\ % h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'#  +37 h +76 .t\CTV.dial :air C'<  +150 h .t\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C'Q +37 h .t\CS.seek 0ml C''4 +507 h '4 C'H& h &W C'Z% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +38 h @ C'5% h %0 .t\CS.seek 5ml C'J'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'W'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'i'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'b& h &W C'z% h %4 .t\CSV.dial CSR C'  +151 h  C'p% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'G'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ +113 h .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'`' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'P  +113 h  C'^% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'M'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +710 h ' ."connect"\CSV.dial CTV C'_  .t\CTV.dial CSV C'N  +37 h  +113  .t\CBV.dial :waste1 C'L @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'X  +37 h  C'|% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'C  +75 h  .t\CS.seek 0ml C' '4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'm  +38 h  C'?% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'/ '9  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'w'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'6'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'r% h %4 .t\CSV.dial :flush C'j  +38 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +38 h @ C'o% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B +113 h .t\CTV.dial :air .\CS.seek 0ml C'c'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +506 h '0 .t\CSV.dial :waste C'8 +38 h .t\CS.seek 0ml C'@'4 +506 h '4 C'y& h &W C'}% h %4 .t\CSV.dial :flush C'V  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'1 @ +37 h @ C'*% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C'd +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'g& h &W C'v% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'h'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'"% h %p .t\CS.seek 7ml C'E'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  '2  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'-'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'[& h &W C'k% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'~'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'0% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'&  +76 h  .t\CS.seek 0ml C'O'4 +766 h '4 #tDisinfecting external Intake with kill C' & h &W C'7% h %4 .t\CSV.dial CSR C't  +37 h  C'Y% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C':'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'3' +416 h ' ."connect"\CSV.dial CBV C'x .t\CBV.dial CSV C'U  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C''' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'u  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '%'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'>'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'T' +1373 h ' .t\CSV.dial CBV C'\ +113 h .t\CS.seek 0ml C']'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&#& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'!( +416 h ( M&H& h &ӷ M&Z% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&J1 +150 h 1 M&W% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&b1 +2 h 1 M&z& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.seek :garage M&G'η +91 h 'η .tHand.close M&\\/ +150 h / M&A& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +264 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'_& h &W .tHand.open M&N1 +150 h 1 M&L% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'X& h &W C'|% h %4 .t\CSV.dial CSR C'  +113 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ','[ +463 h '[ .t\CSV.dial :waste C'm +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '?'4 +2010 h '4 #t\Purging external Intake C' & h &W C'e% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'I  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'w'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'r  +75 h  .t\CS.seek 5ml C'j'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'n% h %p .t\CS.seek 7ml C'o'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +113 h .t\CTV.dial :air .\CS.seek 0ml C'B'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'{'0 +506 h '0 .t\CSV.dial :waste C'c +38 h .t\CS.seek 0ml C'8'4 +506 h '4 C'S& h &W C'y% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.seek 3ml C'V'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'1% h %0 .t\CS.seek 5ml C'*'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'd'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &g(ӷ +6 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'"( +416 h ( M&h& h &ӷ M&4% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +90 h '\  #t\Completed [coreNEG] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST30-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:37:38CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST30-Sep-18" +263667 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep2915h.pcr F*0% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*& % +86 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*U'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*=  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep2917h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +11 h  F*^% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*L  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*X  +21 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*F  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*C  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*m' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*,  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +107 h  ."MFBPCR"delay 7 seconds +700 F*?% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*I n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*r' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*n% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*o % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep2917h.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*{  +22 h ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *}'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *`'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*[  +31 h  ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*)% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep2920h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*%% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*H n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*( I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*J % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*='< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*W  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*z n +21 h n ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*i'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*P'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep2920h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *X'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +21 h F*N% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*C'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*m  +65 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*j'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*S% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18sep2922h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*f  +11 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*2 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*-' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*0 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*q' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*&  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*7  +108 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*'% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CEST1538260192.11 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18sep2922h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*T  +21 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*R'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*J  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*^'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*P'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*L'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18sep3000h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*,  +10 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*w  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*r I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*;% h %Fj ."MFBPCR"\AS.seek 0.524ml F*n' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*l % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.53ml F*o' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*j'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +21 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*@ n +21 h n ."MFBPCR"delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.6ml F*V'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*v% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*` % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18sep3000h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*K  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +150 h F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *[' +424 h ' ."MFBPCR"\ATV.dial :ASV F*g n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*2'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*k n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *0'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*- % +43 h % F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *)' +423 h ' ."MFBPCR"\ATV.dial :air3 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h % H ."MFBPCR"\AS.seek 0.735ml F*Y' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*:% h % Ű ."MFBPCR"\AS.seek 0.72ml F*U' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*u n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*'% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*3% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*#% h % ."MFBPCR"\AS.seek 0.579ml F*>' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %  ."MFBPCR"\AS.seek 0.604ml F*Q'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*R' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*( n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Z  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *5'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*.% h %V ."MFBPCR"\AS.seek 0.01ml F*W' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*=% h % ."MFBPCR"\AS.seek 0.58ml F*z' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h % q ."MFBPCR"\AS.seek 0.605ml F*\\'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*G n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*A  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*^  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *P'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*! n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +202 h 'R ."MFBPCR"\SPE.coast F*|  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*X n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *F' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *,': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*?  +65 h  F*m% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *e'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*/ n +65 h n F*I% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *;': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*l% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *B'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*n n +64 h n F*{% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *S' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*o I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*y  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *j': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*@ n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *}'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*8 n +151 h n ."MFBPCR"\AS.seek 0ml F**'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*1  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*V % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +21 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*f'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*4% h % ."MFBPCR"\AS.seek 0.97ml F*h'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*g I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *k'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*0  +43 h  F*-% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *$' +9988 h ' ."MFBPCR"\ATV.dial AIV F*q I +43 h I ."MFBPCR"\AS.seek 0.02ml F*)' +9880 h ' ."MFBPCR"\ATV.dial :waste F*~ n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*7% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*t  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *:'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*#  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*5'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *P'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*L % +108 h % ."MFBPCR"\AS.seek 0ml F*X'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +3563006 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% %N h  F* *  h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ P% *\ F*\ 9\ P% 0\ F*\ 9\ *F  h  .t\SPE.configure SPEconfig F*\ 2 *]% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S) % h % .t\SS.configure SSconfig C'9& S)#.0X 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:38:38CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'4& h & .tHand.open M&1 +150 h 1 M&g& h &M M&h% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &k'η +91 h 'η .tHand.close M&2/ +150 h / M&0& h &- M&-% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &&(ӷ +264 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C'7(W +416 h (W C')& h &W .tHand.open M&1 +150 h 1 M&t% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'x& h &W C'O% h %4 .t\CSV.dial CSR C'Y  +150 h  C'u% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ':'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'U% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'3& h &W C'% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '#' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T '%  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'<'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'>  +75 h #t\Flushing upper manifold C'H& h &W C'% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C'Z'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +37 h @ C'% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'='4 +507 h '4 C'5& h &W C'p% h %4 .t\CSV.dial :flush C'b  +38 h  .t\CS.seek 3ml C'W'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'z% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'A +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'|( +416 h ( M&!& h &ӷ M&L% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &"'η +91 h 'η .tHand.open M&D1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:38:43CEST26-Sep-18\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:38:38CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*d% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(N. h ., A( & h &  .t\SC.reconfigure SCloose A($.(\ 1~b (F& h &  M&]& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &9( +6 F*C% h %&K ."readyMFB"\ATV.dial :waste2 F*? % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *m' +160 h ( M&,& h & A(/& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &I' +89 h ' A(e& h &  .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(& h &  M&w& h & .t\Elbow.seek 2 M&( +7 h ( M&r& h & A(l& h &  .t\Forearm.seek 2 + 300 counts M&6'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &n/ +150 h / ."downElevator"\SE.seek :down M&{& A('X h & M&B% h %8 .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.seek CC M&8(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'`(W +416 h (W .tHand.open M&K1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)V+ +7 h + S)*% h % C'4& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +75 h @ ."airStroke"\CS.seek 10ml C'H' +144 h  .t\CTV.dial :air C'  +76 h .tdelay 15 seconds +690 h ' +810 .t\SSV.dial :spit,avoiding: :exhaust S)Q +150 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'<  .t\SS.seek 0ml S)R' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)  +144 h  +6  S)Z% h % .t\CBV.dial between SSV and waste2 C'  +19 h  C'(% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'.  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +2609 h '4 C'J% h %4 S)5% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'p @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X9 h 9d +100 C'#9 h 9'd +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9kd +100 C'Q9 h 9d +100 C'<9 h 9d +100 C'9 h 9d +100 C'Z9 h 9d +100 C'9 h 9d +100 C'(9 h 9d +100 C'.9 h 9 d +100 C'9 h 9 d +100 C'H9 h 96d +100 C'J9 h 9Ld +100 C'59 h 9bd +100 C'p9 h 9xd +100 C'R9 h 9d +100 C'=9 h 9d +100 C'i9 h 9d +100 C'z9 h 9d +100 C'b9 h 9d +100 C'W9 h 9d +100 C'\\9 h 9d +100 C'A9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'^9 h 9d +100 C'G9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'79 h 9d +100 C'L9 h 9  d +100 C'_9 h 9 d +100 C'&9 h 9 *d +100 C'X9 h 9 ?d +100 C'a9 h 9 Td +100 C'D9 h 9 j d +100 C'!9 h 9 $d +100 C'9 h 9 (d +100 C'P9 h 9 ,d +100 C'd9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'N9 h 9 , +7 h , .t\Elbow.reconfigure TightElbow A(#- h -+ M&$&.,(\ #b .t\SE.seek 5.04 Pucks below Top Plate A(' +27 h ' M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&Q'o +21 h 'o M&<& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &Z' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(' +36 h ' M&(& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&H(ӷ +142 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&p' +91 h ' .tHand.close M&R/ +150 h / .t\CC.seek :open C'=( +416 h ( A(i& h &( A(z& h &( M&b& h &ӷ M&W% h % .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +142 h ( M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 1 M&'C +93 h 'C .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &^1 +150 h 1 M&G% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M& 'o +21 h 'o M&7& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' A(&% h % ."downElevator"\SE.seek :down A(X'X +100 (a% h % M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'P' +6 h ' .t\CSV.dial between air and CTV C'd ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&N1 +2 h 1 M& & h & M&F% h %\  .t\Elbow.seek FlushPuck::Garage M&s(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&?'η +91 h 'η .tHand.close M&/ +150 h / M&/& h &- M&"% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' .t\CC.seek :closed C'+(W +416 h (W C'& h &W .tHand.open M&{1 +150 h 1 M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%l +151 h P%B% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%9' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P%}' +1597 h ' .t\PSV.dial PSR P%v +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%6  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%S  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%K  +37 h  .t\PS.seek 2.5ml P%|' +2610 h ' .t\PSV.dial :mixloop P%* +38 h .t\PS.seek 0ml P%4' +2609 h ' .t\PSV.dial :air P%;  +38 h  .t\PS.seek 2.5ml P%@' +2610 h ' .t\PSV.dial :mixloop P%h +37 h .t\PS.seek 0ml P%V' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&1& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'o( +416 h ( M&m& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &-(- +265 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &q'η +91 h 'η .tHand.open M&M1 +150 h 1 M&g% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C't& h & M&x% h %\  A(O% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ()( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (~& h &  M&2& h &- M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &'( +166 h ( M& & h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +89 h ' A(j& h &  .t\SE.seek 13.71 Pucks below Top Plate A(u'n +125 h 'n A(%& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &(/ +150 h / ."downElevator"\SE.seek :down M&.& A(3'X h & M&:% h %8 .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.seek CC M&T(ӷ +35 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&Z1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +90 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'`  +19 h  .t\CS.seek 5ml C'i'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'z' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'b'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%p  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%^  +150 h  .t\PS.seek :empty P%G' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'7  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'&  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'\\ ` +18 h ` .t\CSV.dial :air C'A @ +38 h @ .t\CS.seek 10ml C'a' +2647 h ' .t\CSV.dial CTV C'D  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'd @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A( % h %X A(F. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &( M&& h &ӷ M&_% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&C& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &X' +90 h ' A(k& h &( .t\SE.seek 5.71 Pucks below Top Plate A( ' +205 h ' M&& h & A(/& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &"'C +25 h 'C .t\Elbow.hold M&!, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(?- h -+ .t\SE.seek 6.04 Pucks below Top Plate A(' +26 h ' M&w% h %C A(s& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&e'o +21 h 'o M&=& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &{' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(]' +36 h ' M&l& h & M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +89 h '\  .t\Elbow.seek CC M&I(ӷ +143 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.seek CC M&}' +91 h ' .tHand.close M&6/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &( A(S& h &( M&y& h &ӷ M&K% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &*( +142 h ( M&|& h & A(;& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &4'C +93 h 'C .t\Elbow.hold M&h, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &@1 +150 h 1 M&1% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&['o +20 h 'o M&0& h & A(,& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +23 h ' A(+% h % ."downElevator"\SE.seek :down A(m'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%M' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%g +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%t  +37 h .t\CBV.dial AIV C'x  +38 h .t\PRV.dial between CBV and air P%O  +19 h  .t\PRV.dial :air P%Y  +18 h  P%~% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %2' +251 h ' .t\PRV.dial :waste2 P%U @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %q' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&j& h & M&-% h %\  .t\Elbow.seek FlushPuck::Garage M&%(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&E/ +150 h / M&(& h &- M&.% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'u& h &W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  C'o& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Z @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%>  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%`% h % .t\PS.seek 1.499ml P%Q' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%)' +1094 h ' .t\PSV.dial :air P%P  +38 h  .t\PS.seek 0ml P%i' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%z +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%W  +38 h  .tdelay 2 seconds +200 P%V% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%p  +76 h .tdelay 2 seconds +200 P%H% h %r .t\PS.seek 0.475ml P%^' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%7  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %&' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck P%A @ +75 h @ .t\PS.seek 0ml P%b' +2610 h ' .t\PSV.dial :air P%D  +75 h  .t\PS.seek 2.5ml P%a' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%d +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%F  +75 h P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'_ & +50 h & .t\AWV.select :waste C' +7 P%C% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'k { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :PML P%R +38 h .t\PS.seek 0ml P%?' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'w& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C's& F*= @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass F*  .t\PSV.dial PSR P%l +21 h  ."MFBPCR"delay 20 seconds +92 h P%B% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%9  +38 h  .t\PS.seek 0ml P%v' +1597 h ' .t\PSV.dial PSR P%e +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%{ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%!' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*6  +22 h ' .t\PSV.dial PSR P%r +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%S% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%y' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*K % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +37 h  .t\PS.seek 0ml P%}' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*I  +21 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *1' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%0' +216 ."MFBPCR"\ATV.dial :AIV F*, I +43 h I F*+% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%* +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%4% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%]  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*n n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*m% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%G +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%g' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial PSR P%x +113 h P%O% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%~  +38 h  .t\PS.seek 0ml P%M' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%U +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%2' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %j'k +2679 h 'k .t\PSV.dial :waste1 P%-  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *h'V +849 h ' .t\PSV.dial :air P%E  +113 h  .t\PS.seek 2.5ml P%(' +2610 h ' .t\PSV.dial :mixloop P%. +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %$' +5106 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %8' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*J  +43 h  F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *:'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*u% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%3 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %o' +619 h ' ."MFBPCR"\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.seek :empty F*5'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*>  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *`' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*q  +43 h F*Q% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *P'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C')  +38 h  C'z% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'V' +1625 h ' ."connect"\CSV.dial CTV C'i  .t\CTV.dial CSV C'p  +38 h  +113  .t\CBV.dial :waste1 C'H @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*^% h %_ ."MFBPCR"\AS.seek 0.434ml F*7'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'\\'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*A  +22 h F*&% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*D'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*b n +108 h n F*d% h %& ."MFBPCR"\AS.seek 0.092ml F*a'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C' '4 +2647 h '4 C'F& h &W #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&T% h % .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&l1 +150 h 1 M&B% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M& % h %\  A(9% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (X( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (e& h &  M&v& h &- M&{% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&6& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &r' +90 h ' A(S& h &  ."WCR"\SE.seek 12.71 Pucks below Top Plate A("' +136 h ' A(!& h &  M&K& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&;& h & A(I& h &  ."WCR"\Forearm.seek 2 + 300 counts M&@'8 +23 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down A('X M&|& h & M&,% h %8 ."WCR"\Forearm.seek :retract M&+'\ +91 h '\  ."WCR"\Elbow.seek CC M&}(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&1& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +35 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +13 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&]1 +130 h 'R ."MFBPCR"\ATV.dial :air2 F*[  +20 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *m' +89 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)G% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +65 h  F*% h % ."MFBPCR"\AS.seek 0.52ml F*5' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*q n +64 h n ."MFBPCR"\ASV.dial :wasteR F*Q  +21 h  ."MFBPCR"\AS.seek 0.3ml F*`': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*)'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*z % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*W  +87 h F*i% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *p'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*H I +43 h I F*V% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*^ n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*L  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *P' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *A' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*&% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *7' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*b% h % ."MFBPCR"\AS.seek 0.279ml F*d'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*D n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *F'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F*_% h %v ."MFBPCR"\AS.seek 0.059ml F*C'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*k % +21 h % F*'% h %H ."MFBPCR"\AS.seek 0.069ml F*\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F*T' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h % q ."MFBPCR"\AS.seek 0.794ml F*s' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*l% h % k ."MFBPCR"\AS.seek 0.779ml F*B'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*R I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b * ' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*9'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*c % +43 h % F*e% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*6' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %  ."MFBPCR"\AS.seek 0.604ml F*/'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +21 h F*K% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*;' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*I n +86 h n ."MFBPCR"\ASV.dial :wasteR F*?  +43 h  ."MFBPCR"\AS.seek 0ml F*y'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*r% h %V ."MFBPCR"\AS.seek 0.59ml F*1' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +108 h n ."MFBPCR"\ASV.dial :wasteR F*"% h %  ."MFBPCR"\AS.seek 0.09ml F*@' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*+' +625 h ' ."MFBPCR"\ATV.dial :air3 F*a % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h % ."MFBPCR"\AS.seek 0.23ml F*G' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*0'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*X% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*O n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*x  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *Y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*g @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*-  +43 h  #"MFBPCR"\Swish #1 of 2 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*E'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*2% h %(A ."MFBPCR"\AS.seek 0.6ml F*('\  +1031 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*$'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*J% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *#' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*. % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep3017h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*u  +64 h  ."MFBPCR"\ARV.dial :carrier F*3  +11 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +21 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *5'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*`  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*)' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*H % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*P'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*&  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*b' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*7  +43 h  ."MFBPCR"\ASV.dial :waste1 F*D  +65 h ."MFBPCR"\ARV.dial :air1 F*d  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226097 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'_  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'F @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'' @ +75 h @ +50 S)C% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C'% h % ."WCR"\CSV.dial between air and CTV C'\ ` +18 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'w  'T  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'N'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'l  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 's' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'R  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'B  +37 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'c' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)9% h % ."WCR"\CBV.dial between CSV and waste2b C'{  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'v ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'S  '6  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '!'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'K  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '/' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'?  +38 h  C';% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'y' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)r% h % ."WCR"\CBV.dial between CSV and waste2b C',  +94 h  C'*% h % ."WCR"\CSV.dial between air and CTV C'" ` +57 h ` ."WCR"\CS.seek 7.288ml C'1' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'@  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'a' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'+& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A([% h %X A(]. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&G& h &ӷ M&4% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &X( +143 h ( M&0& h & A(O& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(& h &( ."WCR"\SE.seek 6.71 Pucks below Top Plate A(~' +196 h ' M&t& h & A(Y& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C ."WCR"\Elbow.hold M&M, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(-' +27 h ' M&j% h %C A(U& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M& 'o +21 h 'o M&& h & A(2& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &E' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A(<'w +36 h 'w M&(& h & M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +90 h '\  ."WCR"\Elbow.seek CC M&.(ӷ +142 h (ӷ M&#& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&u' +91 h ' ."WCR"Hand.close M&3/ +150 h / ."WCR"\CC.seek :open C':( +416 h ( A(h& h &( A(%& h &( M&m& h &ӷ M&Z% h % ."WCR"\Forearm.seek :retract M&>'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&q& h & A(Q& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C ."WCR"\Elbow.hold M&`, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &z1 +150 h 1 M&5% h %C A(W& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&i'o +21 h 'o M&)& h & A(H& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(% h %w ."downElevator"\SE.seek :down A(^'X +100 (L% h % M&p% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +82 h 'X ."downElevator"\SE.coast A(5 +7 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&&& h & M&A% h %\  .t\Elbow.seek FlushPuck::Garage M&P(- +166 h (- M&7& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.seek :garage M&d'η +91 h 'η .tHand.close M&/ +150 h / M&b& h &- M&\\% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +265 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'T& h &W C'w% h % .t\CSV.dial CSR C'  +38 h  C'l% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'R% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'B'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 's'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'c& h &W C' % h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '{'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'v% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'S  '6  +37 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'K'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'!& h &W C'I% h %4 .t\CSV.dial :flush C'?  +76 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'y% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'r  +37 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C'*'4 +766 h '4 #t\Flushing upper manifold C'"& h &W C'|% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'1 @ +38 h @ C'@% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '+  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C'4& h &W C'0% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C't% h %0 .t\CS.seek 5ml C'Y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'n'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C'U'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'2% h %4 .t\CSV.dial CSR C'(  +151 h  C'J% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'x'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'X' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'~ +112 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'#' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'-  +113 h  C'E% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'M'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '  +37 h  +113  .t\CBV.dial :waste1 C'h @ +38 h @ .t\CS.seek 0ml C'%'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'm  +38 h  C'Z% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'q'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'u  +38 h  .t\CBV.dial :waste2b C'.  +75 h  .t\CS.seek 0ml C'z'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'5  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ')'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'>  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'L'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'&& h &W C'A% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'7% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'V +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'` +38 h .t\CS.seek 0ml C''4 +506 h '4 C'b& h &W C'\\% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'd'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C' % h %0 .t\CS.seek 5ml C'P'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'\ & h &W C'T% h %4 .t\CSV.dial :flush C'w  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C''% h %p .t\CS.seek 7ml C'a'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'R  'N  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'B'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'c& h &W C' % h %4 .t\CSV.dial :flush C'9  +76 h  .t\CS.seek 5ml C's'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'v% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'S  +37 h  .t\CBV.dial :waste2b C'6  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'e& h &W C'!% h %4 .t\CSV.dial CSR C'I  +37 h  C'?% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'K'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'/' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'y ';  +37 h +76 .t\CTV.dial :intake C'r  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C',' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '"'4 +606 h '4 .t\CSV.dial CSR C'|  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'*'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'1' +1373 h ' .t\CSV.dial CBV C'@ +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&+& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&[' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C']( +416 h ( M&4& h &ӷ M&0% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &G(- +264 h (- M&}& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&Y1 +150 h 1 M&n% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&2& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &((- +7 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&x'η +91 h 'η .tHand.close M&~/ +150 h / M&& h &- M&X% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +264 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'$& h &W .tHand.open M&1 +150 h 1 M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'm& h &W C'Z% h %4 .t\CSV.dial CSR C'%  +113 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'u'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '.% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Q'[ +463 h '[ .t\CSV.dial :waste C'5 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +2010 h '4 #t\Purging external Intake C'z& h &W C')% h %4 .t\CSV.dial :flush C'>  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'H  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'L'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'p& h &W C'&% h %4 .t\CSV.dial :flush C'A  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'<% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV C'i .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'V'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'^ +38 h .t\CS.seek 0ml C'`'4 +506 h '4 C'& h &W C'b% h %4 .t\CSV.dial :flush C'\\  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'F% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'd'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&:& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\ (ӷ +6 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C''( +416 h ( M&& h &ӷ M&R% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +90 h 'η .tHand.open M&91 +150 h 1 M&B% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST01-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:40:28CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST01-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118sep3017h25ml.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +86 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +21 h F*I% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %> ."MFBPCR"\AS.seek 0.835ml F*;'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +65 h  F*|% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*4% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Y% h %m ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18sep3019h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ARV.dial :carrier F*-  +11 h  F*E% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *%'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*q  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*.  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.53ml F*5' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Q  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +108 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*p  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18sep3019h25ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*D  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*d'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*:'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*T  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*l% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*c  +31 h  ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*='> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +21 h F*{% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18sep3021h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*"% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *+'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*_  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *C'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*4 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*[' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*} I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Y % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*n  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F*-'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18sep3021h25ml.pcr F*(% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +87 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*i% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V @CEST1538344816.16 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0100h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*l  +42 h  ."MFBPCR"\ARV.dial :carrier F*:  +11 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*c  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\  +22 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *a'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*N I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*v n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*=' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*6  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*S'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*!  +108 h  ."MFBPCR"delay 7 seconds +700 F*s% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*K'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*r' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*;  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0100h25ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*1  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*C'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*0% h %n ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*-'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0102h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*Q  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *&'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*A I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*g % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*D  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +22 h  ."MFBPCR"delay 7 seconds +700 F*b% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*P  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*d  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*T% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0102h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*w @@T *3  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +151 h F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *c' +423 h ' ."MFBPCR"\ATV.dial :ASV F*\ n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*9  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*N'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *v'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*B % +43 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *{' +423 h ' ."MFBPCR"\ATV.dial :air3 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +21 h F*S% h % ."MFBPCR"\AS.seek 0.71ml F*e' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h % H ."MFBPCR"\AS.seek 0.735ml F*?' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*K% h % Ű ."MFBPCR"\AS.seek 0.72ml F*;' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*,% h % zG ."MFBPCR"\AS.seek 0.365ml F*r' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*| % +43 h % F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*+% h % ."MFBPCR"\AS.seek 0.579ml F**' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*k% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +86 h n ."MFBPCR"\ASV.dial :wasteR F*0  +22 h  ."MFBPCR"\AS.seek 0ml F*C'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*[% h %V ."MFBPCR"\AS.seek 0.01ml F*n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h % ."MFBPCR"\AS.seek 0.58ml F*2' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*~'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +21 h F*X% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*G'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *('V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*E  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *-'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*] n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +202 h 'R ."MFBPCR"\SPE.coast F*Z  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*m n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *q' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*u I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*z  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*W  +64 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *)'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*H% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*p  +43 h  F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *i': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*&  +64 h  F*g% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *V'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*< n +65 h n F*D% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*b  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *\\'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*F  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F* % h % ."MFBPCR"\AS.seek 0.96ml F*:'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %0 ."MFBPCR"\AS.seek 0.985ml F*l' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*R% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*\ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *a'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*v  +43 h  F*B% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*= I +43 h I ."MFBPCR"\AS.seek 0.02ml F*{' +9880 h ' ."MFBPCR"\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.seek 0ml F*S'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*!% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*I  +21 h ."MFBPCR"\ATV.dial :air3 F*e % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *K'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +64 h  F*1% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*n'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *-'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*h % +108 h % ."MFBPCR"\AS.seek 0ml F*m'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990711 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )  h  C' '% h  P% % h  F* *u h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *q  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)z% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'>& S)#.0X 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:41:28CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&l1 +150 h 1 M&\ & h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &9(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&N/ +150 h / M&v& h &- M&B% h %η .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +264 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'!(W +416 h (W C'{& h &W .tHand.open M&S1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'y& h &W C'% h %4 .t\CSV.dial CSR C'?  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'K'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ';% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C', +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '|'4 +2009 h '4 #t\Purging external Intake C'/& h &W C'r% h %4 .t\CSV.dial :flush C'@  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '+' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'1 '"  h +75 .t\CTV.dial :intake C'_  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'*  +75 h #t\Flushing upper manifold C'4& h &W C'k% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'0'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'C% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'O'4 +507 h '4 C't& h &W C'J% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'n'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'2% h %0 .t\CS.seek 5ml C'X'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +7 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.seek CC M&$' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&]& h &ӷ M&h% h % .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &''η +91 h 'η .tHand.open M&f1 +150 h 1 M&o% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:41:34CEST26-Sep-18\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:41:28CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'w& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M& % h %\  A(%% h %X A(. h ., A(u& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (q& h &  M&& h &- M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &>( +6 F*.% h %&K ."readyMFB"\ATV.dial :waste2 F*W % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *5' +160 h ( M&Q& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +89 h ' A()& h &  .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ A(& h &  M&L& h & .t\Elbow.seek 2 M&p( +7 h ( M&A& h & A(&& h &  .t\Forearm.seek 2 + 300 counts M&g'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &  .t\CBV.dial SSV +151 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S)H +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'k9 h 9Td +100 C'_9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'C9 h 9d +100 C'09 h 9d +100 C'9 h 9d +100 C'}9 h 9d +100 C'[9 h 9 d +100 C'|9 h 9 d +100 C'49 h 96d +100 C'Y9 h 9Ld +100 C't9 h 9bd +100 C'J9 h 9xd +100 C'9 h 9d +100 C'O9 h 9d +100 C'U9 h 9d +100 C'29 h 9d +100 C'9 h 9d +100 C'n9 h 9d +100 C'~9 h 9d +100 C'9 h 9(d +100 C'G9 h 9>d +100 C'X9 h 9Td +100 C'(9 h 9id +100 C'9 h 9d +100 C'E9 h 9d +100 C'x9 h 9d +100 C'9 h 9d +100 C'e9 h 9d +100 C'!9 h 9d +100 C'h9 h 9  d +100 C'$9 h 9 d +100 C'69 h 9 *d +100 C'89 h 9 ?d +100 C'9 h 9 Td +100 C'f9 h 9 j d +100 C']9 h 9 $d +100 C'j9 h 9 (d +100 C'-9 h 9 ,d +100 C'9 h 9 0d +100 C' 9 h 9 4d +100 C'%9 h 9 8d +100 C'9 h 9 9 h 9 d +100 C'H9 h 9 d +100 C'V9 h 9 d +100 C'T9 h 9 d +100 C'b9 h 9 d +100 C'g9 h 9 d +100 C'9 h 9 1d +100 C'p9 h 9 Dd +100 C'9 h 9 Xd +100 C'D9 h 9 kd +100 C'39 h 9 ~d +100 C'Z9 h 9 d +100 C' 9 h 9 d +100 C'R9 h 9 d .t\CH.hold 5:00,85C C'i   P h  +30000  C'F9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'd'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 5ml C'7'p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'c @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'l  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'v @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'B'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'5 +113 h .t\CBV.dial CSV C'  +19 h C'P% h %4 .t\CS.seek 5ml C'='p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +37 h C'9% h %p .t\CS.seek 7ml C'a'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'M @ +38 h @ .t\CS.seek 10ml C' ' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'I ` +132 h ` #tLysing Complete C'y& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&% h %\  A(?% h %X A({. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (S(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &( M&N& h &ӷ M&;% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &K( +143 h ( M&,& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +89 h ' A(r& h &( .t\SE.seek 7.71 Pucks below Top Plate A(@' +185 h ' M&& h & A("& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &1'C +25 h 'C .t\Elbow.hold M&*, +7 h , .t\Elbow.reconfigure TightElbow A(+- M&$&.,(\ #b h -+ .t\SE.seek 8.04 Pucks below Top Plate A('w +27 h 'w M&k% h %C A(_& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&& h & A(C& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &0' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A('f +36 h 'f M&}& h & M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +90 h '\  .t\Elbow.seek CC M&4(ӷ +142 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&J' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'O( +416 h ( A(U& h &( A(2& h &( M&& h &ӷ M&n% h % .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&G& h & A(X& h &( .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 1 M&('C +93 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &E1 +150 h 1 M&x% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&e'o +21 h 'o M&!& h & A(h& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &$' +22 h ' A(6% h %f ."downElevator"\SE.seek :down A(8'X +100 (% h % M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +72 h 'X ."downElevator"\SE.coast A(5 +17 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'j @ +19 h @ .t\CS.seek 10ml C'-' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'%& h & .tHand.open M&1 +2 h 1 M&u& h & M&q% h %\  .t\Elbow.seek FlushPuck::Garage M&o(- +166 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.seek :garage M&W'η +91 h 'η .tHand.close M&w/ +150 h / M&& h &- M&'% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &A' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C'& h &W .tHand.open M&<1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%& +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%H  +37 h  .t\PS.seek 0ml P%V' +1597 h ' .t\PSV.dial PSR P%T +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%b' +2610 h ' ."connect"\PRV.dial CBV P%g  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P%p +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%3  +38 h  .t\PS.seek 2.5ml P%Z' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%R' +2610 h ' .t\PSV.dial :air P%i  +37 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'7( +416 h ( M&5& h &ӷ M& % h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&M1 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'I& h & M&y% h %\  A(% h %X A(?. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ({( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &  M&N& h &- M&;% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &,( +166 h ( M&& h & A(r& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &`' +90 h ' A(& h &  .t\SE.seek 10.71 Pucks below Top Plate A("' +155 h ' A(& h &  M&+& h & .t\Elbow.seek 2 M&k( +6 h ( M&_& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&C'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &}/ +150 h / ."downElevator"\SE.seek :down A(['X M&/& h & M&K% h %8 .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.seek CC M&1(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&01 +150 h 1 M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'|  +150 h  .t\CSV.dial CTV C'#  +19 h  .t\CS.seek 5ml C'U'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'2' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%n  +37 h  .t\PS.seek 2.5ml P%G' +2610 h ' .t\PSV.dial :mixloop P%X +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%J  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%4 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%E  +150 h  .t\PS.seek :empty P%x' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'!  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'h @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'6  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'~ ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'f  +38 h  .t\CS.seek 0ml C'j'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'%& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&% h %\  A(u% h %X A(q. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (e& h &( M&& h &ӷ M&$% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &z( +142 h ( M&.& h & A(8& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &S' +90 h ' A( & h &( .t\SE.seek 8.71 Pucks below Top Plate A(' +175 h ' M&w& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &''C +25 h 'C .t\Elbow.hold M&], +7 h , .t\Elbow.reconfigure TightElbow A(W- M&$&.,(\ #b h -+ .t\SE.seek 9.04 Pucks below Top Plate A( 'f +27 h 'f M&L% h %C A(o& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &)'o +21 h 'o M&O& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +22 h ' .t\SE.seek 10.04 Pucks below Top Plate A('U +37 h 'U M&&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +89 h '\  .t\Elbow.seek CC M&H(ӷ +142 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&g/ +150 h / .t\CC.seek :open C'( +416 h ( A(p& h &( A(& h &( M&b& h &ӷ M&3% h % .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&Z& h & A(i& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &R'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &F1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&c'o +21 h 'o M&v& h & A(Q& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\\' +22 h ' A(m% h %U ."downElevator"\SE.seek :down A(5'X +100 ( % h % M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +61 h 'X ."downElevator"\SE.coast A(5 +29 h '\  .t\SC.coast .\PSV.dial :air A(6 P%9  +112 h  P%d% h % .t\PS.seek 0.499ml P%M' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%\ +38 h .t\PRV.dial :air P%P  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%I  +38 h .t\CBV.dial AIV C'y  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%?  +18 h  P%s% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %N' +251 h ' .t\PRV.dial :waste2 P%; @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %=' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&r1 +2 h 1 M&& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&a/ +150 h / M&}& h &- M&/% h %η .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'"& h &W .tHand.open M&k1 +150 h 1 M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  C'7& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%0 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%*  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%|  +76 h  .tdelay 2 seconds +200 P%#% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%{' +1094 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 0ml P%U' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%2 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%n  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %X'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%J  +76 h .tdelay 2 seconds +200 P%4% h %r .t\PS.seek 0.475ml P%E' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%G  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%!  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%h  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %6' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%~ +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%f  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%u +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%q  +75 h P%e% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'$ & +50 h & .t\AWV.select :waste C' +7 P%.% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %8'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %1' +1313 h ' .t\PSV.dial :air P%w  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%W +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'L& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'o& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*O @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%& +21 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%S' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*z I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *''6 +66 .t\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%T' +1597 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%< +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%]' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*g  +22 h ' .t\PSV.dial PSR P%A +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*p'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%b' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*3 % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%i  +37 h  .t\PS.seek 0ml P%V' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*H  +21 h  F*F% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%v' +217 ."MFBPCR"\ATV.dial :AIV F*Q I +43 h I F*m% h % ."MFBPCR"\AS.seek 0.469ml F*D' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%l +113 h P%R% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%c' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%9' +739 h ' ."MFBPCR"delay 5 seconds +500 F*d9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*\\ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*5% h % ."MFBPCR"\AS.seek 0.041ml F*('% +336 h ' .t\PSV.dial PSR P%x +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%\ ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%I' +2609 h ' .t\PSV.dial PSR P%y +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%?' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%s  +38 h  .t\PS.seek 0ml P%M' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%; +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%N' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%B  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %+' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*_ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%a  +113 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P%/ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %`' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*Y  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *K'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*"% h %Q ."MFBPCR"\AS.seek 0.01ml F*k' +171 h ' .t\PSV.dial :mixloop P%[ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %7' +619 h ' ."MFBPCR"\ATV.dial :waste F*0 n +128 h n ."MFBPCR"\AS.seek :empty F*t'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F**  +86 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *#' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*=  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *-'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'{  +37 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'n'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  'J  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +38 h @ .t\CS.seek 0ml C'X'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*G  +22 h ."MFBPCR"delay 3 seconds +300 F*E% h %_ ."MFBPCR"\AS.seek 0.434ml F*!'ݏ +110 h '4 .t\CSV.dial CSR C'h  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'~'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*6% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*f'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'%' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'u'4 +2647 h '4 C'q& h &W #tGaraging FlushPuck in Collection Clamp M&e& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'8( +416 h ( M&w& h &ӷ M&1% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'z& h & M&% h %\  A(>% h %X A(^. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (S( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ()& h &  M&T& h &- M&<% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +166 h ( M&g& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &A' +90 h ' A(& h &  ."WCR"\SE.seek 9.71 Pucks below Top Plate A('' +165 h ' A(]& h &  M&3& h & ."WCR"\Elbow.seek 2 M&p( +7 h ( M&i& h & A(H& h &  ."WCR"\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &b/ +89 h '&0 ."MFBPCR"delay 3 seconds +61 h / ."downElevator"\SE.seek :down M&Z& A('X h & M&Q% h %8 ."WCR"\Forearm.seek :retract M&m'\ +91 h '\  ."WCR"\Elbow.seek CC M&V(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +5 ."MFBPCR"\ATV.dial :waste2 F*l % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *R'R +43 h ' ."WCR"\CC.seek :closed C' (W +416 h (W ."WCR"Hand.open M&1 +100 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *d' +28 h 1 M&\\% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)D+ +7 h + S)x% h % C'9& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'P  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*^ % +43 h % F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*g' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*3% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*i' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*H n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*b'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*A% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*l% h %  ."MFBPCR"\AS.seek 0.09ml F*'' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*p I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*m' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*8% h % ."MFBPCR"\AS.seek 0.13ml F*c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.23ml F*x' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h % ."MFBPCR"\AS.seek 0.255ml F*R'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*S% h %X ."MFBPCR"\AS.seek 0.24ml F*D' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *?'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*\ @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*( I +86 h I ."MFBPCR"\AS.seek 0ml F*r'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*;'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*B  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *_'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*a'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*N% h %(A ."MFBPCR"\AS.seek 0.6ml F*}'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*Y% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *+' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*/ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0117h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*"  +64 h  ."MFBPCR"\ARV.dial :carrier F*[  +11 h  F*K% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*d n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*#  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*n  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*j' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*4 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*E  +107 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*G n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*-'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 F*f  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*%'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*e % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226127 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'$  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'q @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C', @ +75 h @ +50 S).% h % ."WCR"\CBV.dial between CSV and waste2b C'C  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'@ ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'L  ."WCR"\CSV.dial CTV C'1  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'&  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'o' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'^' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'z @ +75 h @ +50 S)>% h % ."WCR"\CBV.dial between CSV and waste2b C'<  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'T ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'g  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ']'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'3  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'H  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'i% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'b' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)A% h % ."WCR"\CBV.dial between CSV and waste2b C'Q  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C'l ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'p  'F  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C''  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'8  +151 h C'm& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(\\% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (9(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (P& h &( M&x& h &ӷ M&v% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &S( +143 h ( M&R& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A(D& h &( ."WCR"\SE.seek 9.71 Pucks below Top Plate A(s' +166 h ' M&I& h & A(?& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &('C +25 h 'C ."WCR"\Elbow.hold M&M, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(r- h -+ ."WCR"\SE.seek 10.04 Pucks below Top Plate A(B'U +27 h 'U M&% h %C A(;& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&_& h & A(N& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &a' +22 h ' ."WCR"\SE.seek 11.04 Pucks below Top Plate A('D +36 h 'D M&}& h & M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&/(ӷ +142 h (ӷ M&+& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&"' +91 h ' ."WCR"Hand.close M&[/ +150 h / ."WCR"\CC.seek :open C'K( +416 h ( A(& h &( A(& h &( M&d& h &ӷ M&0% h % ."WCR"\Forearm.seek :retract M&*'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +142 h ( M&=& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &|'C +93 h 'C ."WCR"\Elbow.hold M&#, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &21 +150 h 1 M&t% h %C A(n& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&U'o +21 h 'o M&{& h & A(4& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(j% h %D ."downElevator"\SE.seek :down A(E'X +100 (h% h % M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +52 h 'X ."downElevator"\SE.coast A(5 +37 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'X& h & .tHand.open M&1 +2 h 1 M&6& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&-(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&~% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +264 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C'C& h &W .tHand.open M&w1 +150 h 1 M&@% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'1& h &W C'L% h % .t\CSV.dial CSR C'  +38 h  C'&% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'o'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'^& h &W C'z% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '<'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'T% h %p .t\CS.seek 7ml C')'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'g  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'3'4 +766 h '4 #t\Flushing lower manifold's waste2b port C']& h &W C'H% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C' 'i +415 h 'i ."connect"\CSV.dial CTV C'A  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'Q  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing upper manifold C'l& h &W C'Z% h %4 .t\CSV.dial :flush C'F  +76 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C''% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 'm  +37 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +507 h '0 .t\CSV.dial :waste C'P +37 h .t\CS.seek 0ml C'x'4 +507 h '4 C'v& h &W C'R% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'D'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'I% h %0 .t\CS.seek 5ml C'?'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'r'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C';'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'_& h &W C'N% h %4 .t\CSV.dial CSR C'}  +151 h  C'Y% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'y'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'S' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C's +113 h .t\CTV.dial :intake C'(  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'+' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'B  +113 h  C'a% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'M'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'd  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '='P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  +38 h  .t\CBV.dial :waste2b C'/  +75 h  .t\CS.seek 0ml C'2'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C't  +38 h  C'n% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '{'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '4' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'j '*  +38 h +75 .t\CTV.dial :air C'k  +151 h .t\CS.seek 0ml C'h'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'X +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'6& h &W C'% h %4 .t\CSV.dial :flush C'|  +38 h  .t\CS.seek 5ml C'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'!% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +507 h '0 .t\CSV.dial :waste C'# +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'& h &W C'~% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'-'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'e'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C'C +75 h .t\CS.seek 0ml C'w'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'@& h &W C'1% h %4 .t\CSV.dial :flush C'L  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C',% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'W  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'^& h &W C'z% h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C'o'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'T% h %p .t\CS.seek 7ml C'<'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'g  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tDisinfecting external Intake with kill C')& h &W C']% h %4 .t\CSV.dial CSR C'H  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'3'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'b  'i +37 h +76 .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'Q' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +606 h '4 .t\CSV.dial CSR C'Z  +113 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'' +113 h .t\CS.seek 0ml C'p'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&\\' +91 h ' .tHand.close M&P/ +150 h / .t\CC.seek :open C'c( +416 h ( M&v& h &ӷ M&R% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +264 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &D'η +91 h 'η .tHand.open M&?1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&_1 +2 h 1 M&N& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &}(- +7 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.seek :garage M&y'η +91 h 'η .tHand.close M&s/ +150 h / M&(& h &- M&S% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'& h &W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'd& h &W C'0% h %4 .t\CSV.dial CSR C'  +113 h  C'=% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'"'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '/% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C't +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +2010 h '4 #t\Purging external Intake C'2& h &W C'{% h %4 .t\CSV.dial :flush C'*  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'k .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'4  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'h'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'X  +75 h #t\Flushing upper manifold C'J& h &W C'6% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C'E +38 h .t\CS.seek 0ml C'#'4 +506 h '4 C'9& h &W C'% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.seek 3ml C'G'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'q% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +507 h '0 .t\CSV.dial :waste C'e +75 h .t\CS.seek 0ml C'C'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +6 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.seek CC M&w' +91 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C',( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&>1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST02-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:43:19CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST02-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0117h25ml.pcr F*T% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*g  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *)'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*b'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*A% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +64 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *F'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*P % +86 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +21 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*R'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0119h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ARV.dial :carrier F*B  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*d  +21 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*=  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*/  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.53ml F*t' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +108 h  ."MFBPCR"delay 7 seconds +700 F*n% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*4 n +22 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*X'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*h  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*|'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0119h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*9 % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +21 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +21 h F*-% h %n ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*1  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*L  +65 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +31 h  ."MFBPCR"\ATV.dial :air3 F*@ % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*z% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*g'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Z'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0121h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*F  +10 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *.'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*v n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*V I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*8' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*? % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*r  +107 h  ."MFBPCR"delay 7 seconds +700 F*5% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*N n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*(' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0121h25ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *d'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +21 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +65 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*X  +31 h  ."MFBPCR"\ATV.dial :air3 F*h % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*|'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*9% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *#'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V @CEST1538431216.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0200h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ARV.dial :carrier F*K  +11 h  F*+% h %V ."MFBPCR"\AS.seek 0.5ml F*['(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*W I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*T n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*g'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +108 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0200h25ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*R% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +64 h  F*8% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*; % +86 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*(% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*B'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0202h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*n% h %V ."MFBPCR"\AS.seek 0.5ml F*{'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*X  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*\ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*!' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F*|'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*E  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*#'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.6ml F*G'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*u  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.65ml F*e' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*-  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*1% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*+ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0202h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*L @@T ."MFBPCR"\ASV.dial :SPEdelivery F*[  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *^' +423 h ' ."MFBPCR"\ATV.dial :ASV F*@ n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*>  +43 h  F*w% h % ."MFBPCR"\AS.seek 0.62ml F*W'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*z n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *T'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*%'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *<' +423 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*]' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +21 h F*g% h % ."MFBPCR"\AS.seek 0.71ml F*)' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*3% h % Ű ."MFBPCR"\AS.seek 0.72ml F*b' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Q% h % zG ."MFBPCR"\AS.seek 0.365ml F*A' +7161 h ' ."MFBPCR"\ATV.dial :waste F*F n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*Z % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*m% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*P'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*V n +86 h n ."MFBPCR"\ASV.dial :wasteR F*R  +22 h  ."MFBPCR"\AS.seek 0ml F*.'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*8 % +108 h % F*\\% h %V ."MFBPCR"\AS.seek 0.01ml F*r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*N' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h % q ."MFBPCR"\AS.seek 0.605ml F*s'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +21 h F*S% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*D' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(  +21 h  ."MFBPCR"\AS.seek 0ml F*x'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *B'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*c n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*0  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*d n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *=' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*" I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*2  +43 h  F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*n  +64 h  F*t% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *{'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*j n +65 h n F*4% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *k' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*X I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*J  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*\ % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*f% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *9' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*! I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *|': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *~'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*# n +151 h n ."MFBPCR"\AS.seek 0ml F*u'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*q  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*G % +43 h % F*-% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*e% h % ."MFBPCR"\AS.seek 0.96ml F*K'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %0 ."MFBPCR"\AS.seek 0.985ml F*&' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*@ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *w'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *z'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*T  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *%' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F*<' +9880 h ' ."MFBPCR"\ATV.dial :waste F*o n +129 h n ."MFBPCR"\AS.seek 0ml F*g'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*]% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*H  +22 h ."MFBPCR"\ATV.dial :air3 F*) % +43 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*Q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*'  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*m  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *$'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *B'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*d'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + C'5 M&6 P%5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% %  h  F* *" h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ S)&\ P% *\ C' 2\ P% 0\ .t\SPE.coast F*\ 9\ *\ 9\ *\ 9\ *=  h  .t\SPE.configure SPEconfig F*\ 2 *p% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)2% h % .t\SS.configure SSconfig C'*& S)#.0X 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:44:19CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #5) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&&1 +150 h 1 M&@& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &>(- +166 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &z'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'<& h &W .tHand.open M&g1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'% h %4 .t\CSV.dial CSR C'  +150 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '3'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'b% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'F'[ +463 h '[ .t\CSV.dial :waste C'Q +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +2009 h '4 #t\Purging external Intake C'& h &W C'A% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'l  h +75 .t\CTV.dial :intake C'$  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'v& h &W C' % h %4 .t\CSV.dial :flush C'P  +75 h  .t\CS.seek 5ml C'R'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'.% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 +113 h .t\CTV.dial :air .\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C''4 +507 h '4 C'I& h &W C'Y% h %4 .t\CSV.dial :flush C'_  +38 h  .t\CS.seek 3ml C'r'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'N% h %0 .t\CS.seek 5ml C'S'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C's'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +507 h '0 .t\CSV.dial :waste C'( +75 h .t\CS.seek 0ml C'D'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'0( +416 h ( M&c& h &ӷ M&% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&C1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:44:25CEST26-Sep-18\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:44:19CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'L& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A( . h ., A("& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (=& h &  M&p& h &- M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &*( +6 F*/% h %&K ."readyMFB"\ATV.dial :waste2 F*n % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *t' +160 h ( M&& h & A(j& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &4' +89 h ' A({& h &  .t\SE.seek 8.71 Pucks below Top Plate A(X' +176 h ' A(k& h &  M&& h & .t\Elbow.seek 2 M&h( +6 h ( M&J& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&\ '8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&6& A(f'X h & M&% h %8 .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.seek CC M&#(ӷ +85 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&[1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)G+ +6 h + S)u% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %W .tIntake.select :close S)0 +7 )% h %W .t\SSV.dial :exhaust S)M  +75 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9 ,d +100 C'9 h 9$0d +100 C'M9 h 9<4d +100 C'z9 h 9S8d +100 C'H9 h 9kd +100 C' 9 h 9Td +100 C'$9 h 9kd +100 C'P9 h 9d +100 C'9 h 9d +100 C'.9 h 9d +100 C'R9 h 9d +100 C'89 h 9d +100 C'V9 h 9d +100 C'\\9 h 9 d +100 C'Z9 h 9 d +100 C'v9 h 96d +100 C'?9 h 9Ld +100 C'I9 h 9bd +100 C'Y9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C';9 h 9d +100 C'N9 h 9d +100 C'_9 h 9d +100 C'r9 h 9d +100 C's9 h 9d +100 C'(9 h 9(d +100 C'x9 h 9>d +100 C'S9 h 9Td +100 C'}9 h 9id +100 C'59 h 9d +100 C'a9 h 9d +100 C'y9 h 9d +100 C'9 h 9d +100 C')9 h 9d +100 C']9 h 9d +100 C'9 h 9  d +100 C':9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'd9 h 9 Td +100 C'C9 h 9 j d +100 C'c9 h 9 $d +100 C'9 h 9 (d +100 C'B9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C' 9 h 9 % h %p .t\CS.seek 7ml C''i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'M @ +37 h @ .t\CS.seek 10ml C'z' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'H ` +132 h ` #tLysing Complete C'i& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&% h %\  A(% h %X A(<. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (g(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (o& h &( M&W& h &ӷ M&b% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &3( +143 h ( M&Q& h & A(F& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(A& h &( .t\SE.seek 10.71 Pucks below Top Plate A('' +155 h ' M& & h & A(l& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow A(m- M&$&.,(\ #b h -+ .t\SE.seek 11.04 Pucks below Top Plate A(D'D +27 h 'D M& % h %C A($& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&P'o +21 h 'o M&& h & A(.& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(8'3 +36 h '3 M&V& h & M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +90 h '\  .t\Elbow.seek CC M&v(ӷ +142 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&Y' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(;& h &( A(N& h &( M&_& h &ӷ M&r% h % .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &(( +142 h ( M&x& h & A(S& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &}'C +93 h 'C .t\Elbow.hold M&5, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &a1 +150 h 1 M&y% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&)'o +21 h 'o M&]& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &:' +22 h ' A(% h %3 ."downElevator"\SE.seek :down A('X +100 (d% h % M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C'B' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&"& h & M&=% h %\  .t\Elbow.seek FlushPuck::Garage M&7(- +166 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&L/ +150 h / M&j& h &- M&`% h %η .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &J' +91 h ' .t\CC.seek :closed C',(W +416 h (W C'k& h &W .tHand.open M&1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%6% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%*' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%4  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%1 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%\  C'X  h +75 .t\PSV.dial :mixloop P%h +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%9  h .t\PS.seek 0ml P%f' +2610 h ' .t\PSV.dial :air P%[  +38 h  .t\PS.seek 2.5ml P%0' +2609 h ' .t\PSV.dial :mixloop P%u +38 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%G' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&&' +91 h ' .tHand.close M&T/ +150 h / .t\CC.seek :open C'!( +416 h ( M&& h &ӷ M&e% h % .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +264 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&M1 +150 h 1 M&@% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'H& h & M&i% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (<( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (o& h &  M&W& h &- M&b% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Q( +166 h ( M&F& h & A(A& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &#' +90 h ' A( & h &  .t\SE.seek 7.71 Pucks below Top Plate A(l' +184 h ' A(m& h &  M&|& h & .t\Elbow.seek 2 M& ( +7 h ( M&$& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&.'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down M&& A(\\'X h & M&3% h %8 .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.seek CC M&(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +90 h ' .t\CC.seek :closed C'D(W +416 h (W .tHand.open M&R1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'Z  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C';'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'N' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'_'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%r  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%Y  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%v +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%a  +150 h  .t\PS.seek :empty P%y' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C']  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C's ` +19 h ` .t\CSV.dial :air C'( @ +38 h @ .t\CS.seek 10ml C'd' +2647 h ' .t\CSV.dial CTV C'C  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A("% h %X A(=. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (}(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b ()& h &( M&8& h &ӷ M&:% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &2( +142 h ( M&/& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &g' +90 h ' A(z& h &( .t\SE.seek 11.71 Pucks below Top Plate A(5'Ɛ +145 h 'Ɛ M&L& h & A(j& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &`'C +25 h 'C .t\Elbow.hold M&c, +7 h , .t\Elbow.reconfigure TightElbow A(n- M&$&.,(\ #b h -+Ɛ .t\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M&% h %C A(7& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&{'o +21 h 'o M&& h & A(k& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 13.04 Pucks below Top Plate A(p'" +37 h '" M&& h & M&6% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +89 h '\  .t\Elbow.seek CC M&4(ӷ +142 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'X( +416 h ( A(h& h &( A(9& h &( M&& h &ӷ M&[% h % .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &u( +143 h ( M&0& h & A(U& h &( .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 1 M&'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&q% h %C A(w& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&^'o +21 h 'o M&T& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(,% h %" ."downElevator"\SE.seek :down A('X +100 (e% h % M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +33 h 'X ."downElevator"\SE.coast A(5 +57 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%>  +112 h  P%% h % .t\PS.seek 0.499ml P%M' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%@ +38 h .t\PRV.dial :air P%-  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%H  +38 h .t\CBV.dial AIV C'i  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +18 h  P%o% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %W' +251 h ' .t\PRV.dial :waste2 P%b @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %O' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'F& h & .tHand.open M&A1 +2 h 1 M& & h & M&t% h %\  .t\Elbow.seek FlushPuck::Garage M&m(- +166 h (- M&|& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&V& h &- M&% h %η .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +264 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C'l& h &W .tHand.open M& 1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  C'!& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%R @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%Z  +76 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%P' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%<' +1094 h ' .t\PSV.dial :air P%B  +38 h  .t\PS.seek 0ml P%;' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%N +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%r  +38 h  .tdelay 2 seconds +200 P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%Y  +76 h .tdelay 2 seconds +200 P%v% h %r .t\PS.seek 0.475ml P%a' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%x  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%]  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%s +75 h .t\PTV.dial :PRV,avoiding: :puck P%( @ +75 h @ .t\PS.seek 0ml P%_' +2610 h ' .t\PSV.dial :air P%C  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%" +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%=  +75 h P%)% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %8' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C': & +50 h & .t\AWV.select :waste C' +7 P%/% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'z { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%j' +2610 h ' .t\PSV.dial :PML P%n +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '7& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *k  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +91 h P%6% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*2 I +129 h I F*5% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *`'6 +66 .t\PSV.dial :waste1 P%*  +38 h  .t\PS.seek 0ml P%1' +1597 h ' .t\PSV.dial PSR P%{ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%c' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +22 h ' .t\PSV.dial PSR P%J +21 h  F*D% h %6 ."MFBPCR"\AS.seek 0.329ml F*h'ͯ +92 h P%9% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*[ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*4  +21 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *q' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%w +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%T' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*,% h % ."MFBPCR"\AS.seek 0.469ml F*f' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%u +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%e' +2610 h ' .t\PSV.dial PSR P%& +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%p  +38 h  .t\PS.seek 0ml P%>' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*}'% +336 h ' .t\PSV.dial PSR P%y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%@' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%- +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%H' +2609 h ' .t\PSV.dial PSR P%i +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%o  +38 h  .t\PS.seek 0ml P%M' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%b +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%W' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2679 h 'k .t\PSV.dial :waste1 P%t  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %|' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*$ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%V' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %#' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*?  +43 h  F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *3'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*X I +43 h I F*l% h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +171 h ' .t\PSV.dial :mixloop P%\\ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %!' +619 h ' ."MFBPCR"\ATV.dial :waste F*R n +128 h n ."MFBPCR"\AS.seek :empty F*I'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*Z% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*O  +43 h F*P% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *B'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'<  +37 h  C'N% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'r'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C';  'Y  +38 h  +113  .t\CBV.dial :waste1 C'v @ +38 h @ .t\CS.seek 0ml C'S'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*x  +22 h ."MFBPCR"delay 3 seconds +300 F*a% h %_ ."MFBPCR"\AS.seek 0.434ml F*]'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C's'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*(  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*C'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*_ n +107 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*d'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'"'4 +2647 h '4 C'=& h &W #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&8' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'( +416 h ( M&L& h &ӷ M&% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &j(- +264 h (- M&7& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&1 +150 h 1 M&6% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'2& h & M&5% h %\  A(*% h %X A(E. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (g( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ({& h &  M&1& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&\ & h & A(D& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +90 h ' A(9& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(`' +195 h ' A(c& h &  M&[& h & ."WCR"\Elbow.seek 2 M&h( +7 h ( M&U& h & A(4& h &  ."WCR"\Forearm.seek 2 + 300 counts M& '8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +59 h '&0 ."MFBPCR"delay 3 seconds +91 h / ."downElevator"\SE.seek :down M&0& A(w'X h & M&% h %8 ."WCR"\Forearm.seek :retract M&,'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +13 ."MFBPCR"\ATV.dial :waste2 F*q % +24 h (ӷ M&u& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +19 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +72 h ' ."WCR"\CC.seek :closed C'e(W +416 h (W ."WCR"Hand.open M&p1 +71 h 'R ."MFBPCR"\ATV.dial :air2 F*^  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +57 h 1 M&~% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)f+ +7 h + S)y% h % C'>& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'-  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*-'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*g% h %X ."MFBPCR"\AS.seek 0.24ml F*f' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*i  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*@ @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*} I +86 h I ."MFBPCR"\AS.seek 0ml F*A'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*b'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*t  +43 h  #"MFBPCR"\Swish #1 of 2 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *$'\  +1032 h '\  F*F% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*W% h %(A ."MFBPCR"\AS.seek 0.6ml F*V'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*%'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*?% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *|' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*X  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0217h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*l  +64 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*3% h %V ."MFBPCR"\AS.seek 0.5ml F*m'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +21 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*+  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*r  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*v % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*Y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*x n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*_' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 F*C  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226157 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C':  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'= @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'Q @ +76 h @ +50 S)/% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'' ` +19 h ` ."WCR"\CS.seek 7.288ml C'8' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'7  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'n  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'6  +38 h  C'5% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'E' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'2 @ +75 h @ +50 S)*% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'k% h % ."WCR"\CSV.dial between air and CTV C'1 ` +57 h ` ."WCR"\CS.seek 7.288ml C'D' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\  '9  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'c'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'[  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'j' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'4  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'U% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)J% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'T% h % ."WCR"\CSV.dial between air and CTV C'q ` +57 h ` ."WCR"\CS.seek 7.288ml C'u' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'h  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'w'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'`  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'd' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C',& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&p% h %\  A(~% h %X A(^. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (>(( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&y& h &ӷ M&&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &g( +142 h ( M&-& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &i' +90 h ' A(f& h &( ."WCR"\SE.seek 12.71 Pucks below Top Plate A(o' +136 h ' M&H& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &}'C +25 h 'C ."WCR"\Elbow.hold M&M, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(A- h -+ M&$&.,(\ #b ."WCR"\SE.seek 13.04 Pucks below Top Plate A(t'" +27 h '" M& % h %C A(b& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&F'o +21 h 'o M&$& h & A(W& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 14.04 Pucks below Top Plate A(' +36 h ' M&V& h & M&?% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&|& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ ."WCR"\Forearm.seek CC M&l' +91 h ' ."WCR"Hand.close M&\\/ +150 h / ."WCR"\CC.seek :open C'3( +416 h ( A(& h &( A(m& h &( M&& h &ӷ M&R% h % ."WCR"\Forearm.seek :retract M& '\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&O& h & A(P& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &Z'C +93 h 'C ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &N1 +150 h 1 M&I% h %C A(r& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&;'o +21 h 'o M&<& h & A(v& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &G' +22 h ' A(% h % ."downElevator"\SE.seek :down A(a'X +100 (% h %v M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&1 +2 h 1 M&& h & M&(% h %\  .t\Elbow.seek FlushPuck::Garage M&B(- +166 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&_& h &- M&s% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +264 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'/(W +416 h (W C'.& h &W .tHand.open M&L1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'7& h &W C'% h % .t\CSV.dial CSR C'8  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '6'[ +463 h '[ .t\CSV.dial :waste C'5 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'2% h %4 .t\CSV.dial :flush C'*  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'1% h %p .t\CS.seek 7ml C'{'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'9  '\  +38 h  +113  .t\CBV.dial :waste1 C'D @ +75 h @ .t\CS.seek 0ml C'['4 +766 h '4 #t\Flushing lower manifold's waste2b port C'c& h &W C'4% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'J  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'T'4 +766 h '4 #t\Flushing upper manifold C'q& h &W C'0% h %4 .t\CSV.dial :flush C'h  +76 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'`% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' ',  +37 h +75 .t\CTV.dial :air C'p  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'y'4 +507 h '4 C'&& h &W C'-% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'f'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'H% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'A'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'b +76 h .t\CS.seek 0ml C''4 +506 h '4 #tDisinfecting external Intake with mfbkill C'$& h &W C'W% h %4 .t\CSV.dial CSR C'V  +151 h  C'?% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'i'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'g' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'o +113 h .t\CTV.dial :intake C'}  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'|' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'X  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'F'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C't  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'M'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +709 h ' ."connect"\CSV.dial CTV C'%  .t\CTV.dial CSV C'e  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'm'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'O'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'N'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'I  +38 h  C'r% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C'S +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'& h &W C'(% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C']% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV C'G .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'a'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'>'0 +507 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C''4 +506 h '4 C'_& h &W C's% h %4 .t\CSV.dial :flush C'x  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'"% h %0 .t\CS.seek 5ml C'B'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C')'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'3'0 +507 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C'L'4 +507 h '4 #t\Flushing lower manifold's waste1 port C''& h &W C'7% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'8'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'Q% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  '  +38 h  +113  .t\CBV.dial :waste1 C'5 @ +37 h @ .t\CS.seek 0ml C'6'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'E& h &W C'2% h %4 .t\CSV.dial :flush C'*  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'1% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'9  +37 h  .t\CBV.dial :waste2b C'\  +76 h  .t\CS.seek 0ml C'D'4 +765 h '4 #tDisinfecting external Intake with kill C'{& h &W C'c% h %4 .t\CSV.dial CSR C'4  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'['L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'j' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U '  +37 h +76 .t\CTV.dial :intake C'J  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'q'4 +606 h '4 .t\CSV.dial CSR C'0  +113 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'T'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'u' +1372 h ' .t\CSV.dial CBV C'` +113 h .t\CS.seek 0ml C' '4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'^( +416 h ( M&&& h &ӷ M&-% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &f'η +91 h 'η .tHand.open M&1 +150 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&$1 +2 h 1 M&W& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &V(- +7 h (- M&?& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&i'η +91 h 'η .tHand.close M&o/ +150 h / M&}& h &- M&g% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +264 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'%& h &W .tHand.open M&e1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'R% h %4 .t\CSV.dial CSR C'm  +113 h  C'O% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'P'[ +462 h '[ .t\CSV.dial :waste C'I +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'r'4 +2010 h '4 #t\Purging external Intake C'N& h &W C'<% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'v  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'Y& h &W C'% h %4 .t\CSV.dial :flush C'(  +76 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'G'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'a +38 h .t\CS.seek 0ml C'+'4 +506 h '4 C'>& h &W C'_% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C'x'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'=% h %0 .t\CS.seek 5ml C'"'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +507 h '0 .t\CSV.dial :waste C') +75 h .t\CS.seek 0ml C'.'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&3& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +6 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'Q( +416 h ( M&8& h &ӷ M&n% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +264 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &2'η +91 h 'η .tHand.open M&*1 +150 h 1 M&6% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST03-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:46:12CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST03-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0217h25ml.pcr F*1% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*9  +22 h ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*0% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *h'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*w  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*:% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +21 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*-'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *b'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0219h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*V'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*^ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*O  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*F% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.53ml F*I' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*P  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.536ml F*<'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*r% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*(' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Z'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0219h25ml.pcr F*G% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*C  +22 h ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *s'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +21 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*7  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *|'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +31 h  ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0221h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*h  +10 h  F*q% h %V ."MFBPCR"\AS.seek 0.5ml F*T'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*u n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *,'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*:  +22 h ."MFBPCR"delay 2 seconds +200 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b */'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*& n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*i% h % \\ ."MFBPCR"\AS.seek 0.326ml F*t'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0221h25ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +21 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +21 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*N  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V @CEST1538517616.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0300h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*3  +11 h  F*|% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*6' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*1 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*9'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*4'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*{ n +21 h n ."MFBPCR"delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*J' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0300h25ml.pcr F*0% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*u  +22 h ."MFBPCR"\ATV.dial :air3 F*` % +86 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*p% h %Z ."MFBPCR"\AS.seek 0.81ml F*w'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*-% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*%% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *R'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0302h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +11 h  F*r% h %V ."MFBPCR"\AS.seek 0.5ml F*<'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*( I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*;% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*@ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*]' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +22 h  ."MFBPCR"delay 7 seconds +700 F*_% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*x'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*"  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*B  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*7% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0302h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *\\  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +151 h F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *E' +423 h ' ."MFBPCR"\ATV.dial :ASV F*' n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F**  +43 h  F*L% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*2 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 0ml F*1'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*9% h % ."MFBPCR"\AS.seek 0.71ml F*{' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*[% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*J' +7161 h ' ."MFBPCR"\ATV.dial :waste F*h n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*0 % +43 h % F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*,% h % ."MFBPCR"\AS.seek 0.579ml F*T' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*z% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*w' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*-  +22 h  ."MFBPCR"\AS.seek 0ml F*/'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *H'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*~% h %V ."MFBPCR"\AS.seek 0.01ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*W' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h % q ."MFBPCR"\AS.seek 0.605ml F*o'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*g% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*f' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*i n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*}  +21 h  ."MFBPCR"\AS.seek 0ml F*y'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *V'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *X' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*% n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *t'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*^ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*R  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*F  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *O' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*l I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*N  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *P': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*r  +64 h  F*I% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *<'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*v% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*S I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Y  +43 h  F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *;': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*@% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *G'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*C% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *>' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*_  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Z': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *s'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*+ n +151 h n ."MFBPCR"\AS.seek 0ml F*"'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*=  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*x % +43 h % F*B% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*)% h % ."MFBPCR"\AS.seek 0.96ml F*3'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*n% h % ."MFBPCR"\AS.seek 0.97ml F*8'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*' I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *2'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*1  +43 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*9'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*c% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*4  +22 h ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +64 h  F*u% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *:'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*w % +86 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*~% h % ."MFBPCR"\AS.seek 0.81ml F*H'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *t'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )F h  C' 'm h  P% %e h  F* *l h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *O  h  .t\SPE.configure SPEconfig F*\ 2 * % h %V .t\AS.configure ASconfig S)N% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X/ .t\Elbow.jog 1500 M&_* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b 'a'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:47:13CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #6) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&1 +150 h 1 M&'& h &M M&8% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &*(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &2'η +91 h 'η .tHand.close M& / +150 h / M&1& h &- M&6% h %η .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\ (ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'c(W +416 h (W C'& h &W .tHand.open M&91 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'U& h &W C'D% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '['p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'h'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +2009 h '4 #t\Purging external Intake C'j& h &W C'J% h %4 .t\CSV.dial :flush C'`  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u 'q  h +75 .t\CTV.dial :intake C':  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'p'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'T  +75 h #t\Flushing upper manifold C'&& h &W C'z% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'-'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +37 h @ C'/% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'H& h &W C'?% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'A'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'W% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'o'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'i'0 +507 h '0 .t\CSV.dial :waste C'} +75 h .t\CS.seek 0ml C'f'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&X/ +150 h / .t\CC.seek :open C'R( +416 h ( M&^& h &ӷ M&% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Q'η +91 h 'η .tHand.open M&K1 +150 h 1 M&!% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:47:19CEST26-Sep-18\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:47:13CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*.% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&F% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(m% h %X A(e. h ., A(l& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (O& h &  M& & h &- M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*r % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *I' +160 h ( M&P& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &v' +89 h ' A(<& h &  .t\SE.seek 5.71 Pucks below Top Plate A(S' +205 h ' A( & h &  M&& h & .t\Elbow.seek 2 M&Y( +7 h ( M&(& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&@'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(C'X M&& h & M&G% h %8 .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.seek CC M&+(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'|(W +415 h (W .tHand.open M&\\1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)x+ +7 h + S)"% h % C'n& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % .t\CS.seek 6ml C'R' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'\\ +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b ';' +709 h ' .t\CSV.dial CSR C'"  +151 h  C'n% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '' +479 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '=% h %  .t\CS.seek 4.5ml C''` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'x  +37 h  .t\CS.seek 1.275ml C'8' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C''   ']9 h 9d +100 C'L9 h 9d +100 C'E9 h 91d +100 C'9 h 9J d +100 C'19 h 9bd +100 C'I9 h 9zd +100 C'69 h 9d +100 C')9 h 9d +100 C'B9 h 9 d +100 C'9 h 9$d +100 C'9 h 9(d +100 C'*9 h 9 ,d +100 C'd9 h 9$0d +100 C'M9 h 9<4d +100 C'29 h 9S8d +100 C'49 h 9kd +100 C'z9 h 9Td +100 C'u9 h 9kd +100 C'9 h 9d +100 C'p9 h 9d +100 C'/9 h 9d +100 C'-9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'~9 h 9 d +100 C'09 h 9 d +100 C'&9 h 96d +100 C'9 h 9Ld +100 C'H9 h 9bd +100 C'?9 h 9xd +100 C'w9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'W9 h 9d +100 C'$9 h 9d +100 C'A9 h 9d +100 C'o9 h 9d +100 C'}9 h 9(d +100 C'y9 h 9>d +100 C'g9 h 9Td +100 C'V9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'i9 h 9d +100 C'X9 h 9d +100 C'{9 h 9d +100 C'c9 h 9d +100 C'9 h 9  d +100 C'#9 h 9 d +100 C'\ 9 h 9 *d +100 C'%9 h 9 ?d +100 C'k9 h 9 Td +100 C'K9 h 9 j d +100 C'^9 h 9 $d +100 C'b9 h 9 (d +100 C't9 h 9 ,d +100 C'.9 h 9 0d +100 C'F9 h 9 4d +100 C'm9 h 9 8d +100 C'e9 h 9 9 h 9 Xd +100 C'C9 h 9 kd +100 C'\\9 h 9 ~d +100 C'R9 h 9 d +100 C'"9 h 9 d +100 C'n9 h 9 d .t\CH.hold 5:00,85C C';   P h  +30000  C'=9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +423 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'x @ +113 h @ .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 5ml C']'p +1372 h 'p .t\CSV.dial CTV C'L  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'E @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'1 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'I'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'6 +113 h .t\CBV.dial CSV C')  +19 h C'B% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C'*% h %p .t\CS.seek 7ml C'd'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'M @ +38 h @ .t\CS.seek 10ml C'2' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'4 ` +131 h ` #tLysing Complete C'U& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&D% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (9(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M& & h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &[( +142 h ( M&& h & A(h& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &j' +90 h ' A(J& h &( .t\SE.seek 13.71 Pucks below Top Plate A(`'n +125 h 'n M&& h & A(q& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &:'C +25 h 'C .t\Elbow.hold M&T, +7 h , .t\Elbow.reconfigure TightElbow A(,- M&$&.,(\ #b h -+n .t\SE.seek 14.04 Pucks below Top Plate A(f' +27 h ' M&z% h %C A(u& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&p& h & A(/& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &-' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(' +36 h ' M&& h & M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +90 h '\  .t\Elbow.seek CC M&&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&w/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &( A(W& h &( M&$& h &ӷ M&A% h % .t\Forearm.seek :retract M&o'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &}( +142 h ( M&y& h & A(g& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &V'C +93 h 'C .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&i% h %C A(X& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&{'o +21 h 'o M&c& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &#' +22 h ' A(\ % h % ."downElevator"\SE.seek :down A(%'X +100 (k% h %- M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'b @ +19 h @ .t\CS.seek 10ml C't' +7 h ' .t\CSV.dial between air and CTV C'. ` +18 h ` .t\AIV.dial :bypass C'F { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'm& h & .tHand.open M&e1 +2 h 1 M&l& h & M&O% h %\  .t\Elbow.seek FlushPuck::Garage M&!(- +166 h (- M&N& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&Q% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +265 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%( +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +37 h  .t\PS.seek 0ml P%G' +1597 h ' .t\PSV.dial PSR P%7 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%_' +2610 h ' ."connect"\PRV.dial CBV P%@  .t\CBV.dial PRV C'S  h +75 .t\PSV.dial :mixloop P%Y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%>  h .t\PS.seek 0ml P%C' +2610 h ' .t\PSV.dial :air P%\\  +38 h  .t\PS.seek 2.5ml P%R' +2609 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.seek 0ml P%n' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%=' +2610 h ' .t\PSV.dial :mixloop P%8 +38 h .t\PS.seek 0ml P%x' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&1/ +150 h / .t\CC.seek :open C']( +416 h ( M&6& h &ӷ M&)% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &I(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&M1 +150 h 1 M&'% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'4& h & M&U% h %\  A(D% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M& & h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&h& h & A(J& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +90 h ' A(& h &  .t\SE.seek 4.71 Pucks below Top Plate A(q' +214 h ' A(,& h &  M&z& h & .t\Elbow.seek 2 M&u( +7 h ( M&p& h & A(/& h &  .t\Forearm.seek 2 + 300 counts M&Z'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(~'X M&j& h & M&[% h %8 .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.seek CC M&:(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +19 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'f(W +416 h (W .tHand.open M&-1 +150 h 1 M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'0  +151 h  .t\CSV.dial CTV C'|  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'W' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'$'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%A  +38 h  .t\PS.seek 2.5ml P%y' +2609 h ' .t\PSV.dial :mixloop P%g +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%?  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%& +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%i' +2610 h ' .t\CBV.dial :waste1 C'X @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'c  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'o ` +19 h ` .t\CSV.dial :air C'} @ +38 h @ .t\CS.seek 10ml C'k' +2647 h ' .t\CSV.dial CTV C'K  +37 h  .t\CS.seek 0ml C'b'4 +2648 h '4 .t\CBV.dial :waste1 C'. @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'F  +151 h C'm& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&e% h %\  A(l% h %X A(O. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (V(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b ({& h &( M&& h &ӷ M&#% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &N( +143 h ( M&& h & A(%& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &9' +89 h ' A(2& h &( .t\SE.seek 14.71 Pucks below Top Plate A( '] +116 h '] M&& h & A( & h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &Q'C +25 h 'C .t\Elbow.hold M&^, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(r- h -+] .t\SE.seek 15.04 Pucks below Top Plate A(5' +27 h ' M&% h %C A(!& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&<'o +21 h 'o M&& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 16.04 Pucks below Top Plate A( ' +37 h ' M&(& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&v(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&G' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'S( +416 h ( A(Y& h &( A(>& h &( M&_& h &ӷ M&\\% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &"( +143 h ( M&R& h & A(;& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &n'C +92 h 'C .t\Elbow.hold M&8, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &=1 +150 h 1 M&% h %C A(L& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &E'o +21 h 'o M&1& h & A(P& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' A(% h % ."downElevator"\SE.seek :down A(6'X +100 ()% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%*  +112 h  P%% h % .t\PS.seek 0.499ml P%M' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%' +38 h .t\PRV.dial :air P%B  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%4  +38 h .t\CBV.dial AIV C'U  +38 h .t\PRV.dial between CBV and air P%D  +19 h  .t\PRV.dial :air P%  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'h& h & .tHand.open M&J1 +2 h 1 M&& h & M&I% h %\  .t\Elbow.seek FlushPuck::Garage M&d(- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.seek :garage M&p'η +91 h 'η .tHand.close M&// +150 h / M&& h &- M&j% h %η .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C'q& h &W .tHand.open M&u1 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  C']& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%- @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%T  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%0  +76 h  .tdelay 2 seconds +200 P%|% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 0ml P%' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%W +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%A  +38 h  .tdelay 2 seconds +200 P%x% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %g'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%?  +76 h .tdelay 2 seconds +200 P%&% h %r .t\PS.seek 0.475ml P%' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%y  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%X'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%c  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%o +75 h .t\PTV.dial :PRV,avoiding: :puck P%} @ +75 h @ .t\PS.seek 0ml P%$' +2610 h ' .t\PSV.dial :air P%K  +75 h  .t\PS.seek 2.5ml P%k' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%. +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%F' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%m  +75 h  .t\PS.seek 2.5ml P%e' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%l +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%O  +75 h P%{% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'# & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %%'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'2 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :PML P%r +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '!& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *w  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%( +21 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%9' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*N I +129 h I F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Q'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%7' +1597 h ' .t\PSV.dial PSR P%< +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%5' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%^' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +22 h ' .t\PSV.dial PSR P% +21 h  F*f% h %6 ."MFBPCR"\AS.seek 0.329ml F*Y'ͯ +92 h P%>% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%_' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*\\ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +37 h  .t\PS.seek 0ml P%G' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*v  +21 h  F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%L +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%1' +217 ."MFBPCR"\ATV.dial :AIV F*P I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*C' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%" +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%)' +2610 h ' .t\PSV.dial PSR P% +113 h P%n% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%E' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%*' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*s n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*6% h % ."MFBPCR"\AS.seek 0.041ml F*V'% +336 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%'' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%B +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%4' +2609 h ' .t\PSV.dial PSR P%U +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%M' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%I  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %,' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*z I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *8'V +849 h ' .t\PSV.dial :air P%/  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%p  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %+' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *['Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*S I +43 h I F*q% h %Q ."MFBPCR"\AS.seek 0.01ml F*u' +171 h ' .t\PSV.dial :mixloop P%~ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %]' +619 h ' ."MFBPCR"\ATV.dial :waste F*- n +128 h n ."MFBPCR"\AS.seek :empty F*H'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*T  +86 h  F*0% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *|' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'W% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'A'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'x' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'?  +38 h  +113  .t\CBV.dial :waste1 C'& @ +38 h @ .t\CS.seek 0ml C'g'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*y  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*c'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'X% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'o'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*}  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*K'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*$ n +107 h n F*.% h %& ."MFBPCR"\AS.seek 0.092ml F*k'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'm' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'F  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'l'4 +2647 h '4 C'O& h &W #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &e(ӷ +7 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C'%( +416 h ( M&& h &ӷ M&:% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &w'η +91 h 'η .tHand.open M&(1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'N& h & M& % h %\  A(% h %X A(a. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (9( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (<& h &  M&7& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&@& h & A(f& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(>& h &  ."WCR"\SE.seek 3.71 Pucks below Top Plate A(Q' +223 h ' A(^& h &  M&\\& h & ."WCR"\Elbow.seek 2 M&Y( +7 h ( M&;& h & A(v& h &  ."WCR"\Forearm.seek 2 + 300 counts M&='8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &_/ +31 h '&0 ."MFBPCR"delay 3 seconds +119 h / ."downElevator"\SE.seek :down A(R'X M&L& h & M&P% h %8 ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&G(ӷ +90 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R h 'X ."downElevator"\SE.coast A(5 +9 h (ӷ M&1& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' ."WCR"\CC.seek :closed C')(W +416 h (W ."WCR"Hand.open M& 1 +43 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +85 h 1 M&s% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)C+ +7 h + S)i% h % C'*& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'B  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*^% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*\\' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*; n +86 h n ."MFBPCR"\ASV.dial :wasteR F*v  +43 h  ."MFBPCR"\AS.seek 0ml F*_'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *P'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*5 % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*1 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*=' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*Q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*Y I +86 h I F*k% h % ."MFBPCR"\AS.seek 0.12ml F*R' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*%% h % ."MFBPCR"\AS.seek 0.13ml F*E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*s% h % ."MFBPCR"\AS.seek 0.23ml F*i' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h % ."MFBPCR"\AS.seek 0.255ml F*B'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*9% h %X ."MFBPCR"\AS.seek 0.24ml F*C' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*D n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*' @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*V I +86 h I ."MFBPCR"\AS.seek 0ml F*J'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*I  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *z'\  +1032 h '\  F*h% h %\  ."MFBPCR"\AS.seek 0.5ml F*/'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*p% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *,' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*j % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +3472 h 'V ."MFBPCR"\SPE.coast F*S  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0317h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*q  +64 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*[% h %V ."MFBPCR"\AS.seek 0.5ml F*d'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *H'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*|  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*W% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*A  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*& % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*x% h % ."MFBPCR"\AS.seek 0.536ml F*?'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*X'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*t'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*$' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*c  +43 h  ."MFBPCR"\ASV.dial :waste1 F*K  +65 h ."MFBPCR"\ARV.dial :air1 F*.  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*m'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*{ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226185 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'#  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'O @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'Z  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'` ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'!  ':  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'e'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'(  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'r  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'a' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'N @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'7 ` +57 h ` ."WCR"\CS.seek 7.288ml C'f' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'>  '@  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'^  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C';  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'v  +38 h  C'\\% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'_' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'P  +95 h  C'1% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Q  'Y  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'k'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'=  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'%  +151 h C'R& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M& % h %\  A(s% h %X A(E. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (*(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ("& h &( M&i& h &ӷ M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &9( +142 h ( M&B& h & A(D& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &U' +90 h ' A(C& h &( ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +106 h 'L M&4& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &V'C +26 h 'C ."WCR"\Elbow.hold M&M, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(J- M&$&.,(\ #b h -+L ."WCR"\SE.seek 16.04 Pucks below Top Plate A(I' +27 h ' M&% h %C A(& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&h'o +20 h 'o M&z& h & A( & h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' ."WCR"\SE.seek 17.04 Pucks below Top Plate A(p' +37 h ' M&& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&j(ӷ +143 h (ӷ M&,& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ ."WCR"\Forearm.seek CC M&q' +91 h ' ."WCR"Hand.close M&~/ +150 h / ."WCR"\CC.seek :open C'[( +415 h ( A(8& h &( A(d& h &( M&& h &ӷ M&-% h % ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &u( +143 h ( M&& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &0'C +92 h 'C ."WCR"\Elbow.hold M&|, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &W1 +150 h 1 M&H% h %C A(A& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(&& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &x' +22 h ' A(b% h % ."downElevator"\SE.seek :down A('X +93 h 'X ."downElevator"\SE.coast A(5 +7 (% h %X M&?% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&X1 +2 h 1 M&\ & h & M&}% h %\  .t\Elbow.seek FlushPuck::Garage M&t(- +166 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&.'η +91 h 'η .tHand.close M&F/ +150 h / M&$& h &- M&o% h %η .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'Z& h &W .tHand.open M&1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'!% h % .t\CSV.dial CSR C'  +37 h  C'(% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'e'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'N% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'7% h %p .t\CS.seek 7ml C'<'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  '>  +38 h  +112  .t\CBV.dial :waste1 C'f @ +75 h @ .t\CS.seek 0ml C'^'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C';% h %4 .t\CSV.dial :flush C'v  +75 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'_% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'P  +75 h  .t\CS.seek 0ml C'1'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'L% h %4 .t\CSV.dial :flush C'Y  +75 h  .t\CS.seek 5ml C'Q'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'=% h %p .t\CS.seek 7ml C'k'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'% 'R  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C's'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +507 h '0 .t\CSV.dial :waste C'" +38 h .t\CS.seek 0ml C'i'4 +506 h '4 C'n& h &W C'B% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 3ml C'C'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'4% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'J'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'z'4 +507 h '4 #tDisinfecting external Intake with mfbkill C' & h &W C'6% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'9' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'V  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C',' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'h'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'I  +113 h  C'/% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'M'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C')  +38 h  +113  .t\CBV.dial :waste1 C'8 @ +37 h @ .t\CS.seek 0ml C'd'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'-% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'q  +37 h  .t\CBV.dial :waste2b C'j  +76 h  .t\CS.seek 0ml C'W'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'H  +38 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'b 'T  +38 h +75 .t\CTV.dial :air C'u  +151 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +506 h '0 .t\CSV.dial :waste C'g +38 h .t\CS.seek 0ml C'X'4 +506 h '4 #t\Flushing upper manifold C'\ & h &W C'}% h %4 .t\CSV.dial :flush C'0  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +38 h @ C'c% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'x'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'| +38 h .t\CS.seek 0ml C'F'4 +507 h '4 C'$& h &W C'o% h %4 .t\CSV.dial :flush C'y  +37 h  .t\CS.seek 3ml C'.'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +37 h @ C'l% h %0 .t\CS.seek 5ml C't'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'{'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +506 h '0 .t\CSV.dial :waste C'Z +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'`& h &W C':% h %4 .t\CSV.dial :flush C'!  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'r  'e  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'N% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'7% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +38 h  .t\CBV.dial :waste2b C'>  +75 h  .t\CS.seek 0ml C'f'4 +766 h '4 #tDisinfecting external Intake with kill C'<& h &W C'% h %4 .t\CSV.dial CSR C';  +38 h  C'v% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'^'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'_ '\\  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'P' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'5  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'L  +113 h  C'Y% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'1'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'= +113 h .t\CS.seek 0ml C'Q'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&"/ +150 h / .t\CC.seek :open C'E( +415 h ( M&n& h &ӷ M&B% h % .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &i(- +265 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&1 +150 h 1 M&J% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&6& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.seek :garage M&U'η +91 h 'η .tHand.close M&/ +150 h / M&V& h &- M&9% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'& h &W .tHand.open M&)1 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'-% h %4 .t\CSV.dial CSR C'd  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'q'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'H +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'A'4 +2010 h '4 #t\Purging external Intake C'W& h &W C'% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'b' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u +75 h .t\CTV.dial :intake C'&  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'g  +76 h #t\Flushing upper manifold C'?& h &W C'\ % h %4 .t\CSV.dial :flush C'}  +75 h  .t\CS.seek 5ml C'0'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'p% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +506 h '0 .t\CSV.dial :waste C'x +38 h .t\CS.seek 0ml C'|'4 +507 h '4 C'*& h &W C'$% h %4 .t\CSV.dial :flush C'o  +37 h  .t\CS.seek 3ml C'y'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +37 h @ C'O% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'{ +76 h .t\CS.seek 0ml C'Z'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&(/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&r% h % .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &N'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST04-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:49:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST04-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0317h25ml.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*v'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*P  +64 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*n% h % ."MFBPCR"\AS.seek 0.81ml F*B'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0319h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +11 h  F*/% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*8  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*j  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*H' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*W% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +108 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*& n +22 h n ."MFBPCR"delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*X  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*p% h % \\ ."MFBPCR"\AS.seek 0.326ml F*0'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*c % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0319h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*K  +22 h ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +21 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +21 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +65 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +31 h  ."MFBPCR"\ATV.dial :air3 F*` % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*N% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +21 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0321h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*Y  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*1'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*"  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*#  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*n n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*s' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*G I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.53ml F*%' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*z'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*C  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*V' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*9  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*i  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0321h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +21 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +21 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*W  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*g  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*c'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*0'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F**% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*x  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V @CEST1538604016.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0400h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ARV.dial :carrier F*[  +11 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*a  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*`  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*e I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*7 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*m% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*>  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*< n +21 h n ."MFBPCR"delay 2 seconds +200 F*f% h %\  ."MFBPCR"\AS.seek 0.6ml F*^'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*5  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*P% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Y  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0400h25ml.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *"'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +64 h  F*%% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0402h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*g  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*} I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*' % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*K  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*x  +22 h  ."MFBPCR"delay 7 seconds +700 F*$% h %< ."MFBPCR"\AS.seek 0.594ml F*|'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +22 h n ."MFBPCR"delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*.  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*('{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0402h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*! @@T ."MFBPCR"\ASV.dial :SPEdelivery F*~  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +151 h F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *a' +423 h ' ."MFBPCR"\ATV.dial :ASV F*` n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*e'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*N n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *7'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*m'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*@% h % ."MFBPCR"\AS.seek 0.71ml F*<' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h % H ."MFBPCR"\AS.seek 0.735ml F*v' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*^% h % Ű ."MFBPCR"\AS.seek 0.72ml F*\\' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*5 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*P% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*Y n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*L % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*R% h % ."MFBPCR"\AS.seek 0.579ml F*1' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*"'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*2% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*G n +86 h n ."MFBPCR"\ASV.dial :wasteR F*B  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *4'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*% % +108 h % F*s% h %V ."MFBPCR"\AS.seek 0.01ml F*J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*D% h % ."MFBPCR"\AS.seek 0.58ml F*6' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +21 h F*9% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*C' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*U n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*V  +21 h  ."MFBPCR"\AS.seek 0ml F*i'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*/  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *S' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *I'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*E n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +202 h 'R ."MFBPCR"\SPE.coast F*-  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*h  +43 h  F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*W  +43 h  F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*A  +64 h  F*H% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*b n +65 h n F*&% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *u' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*g I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*?  +43 h  F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*\  +65 h  F*'% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*p n +65 h n F*K% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b **' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*c I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*$  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *0': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*F n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *o'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*| n +151 h n ."MFBPCR"\AS.seek 0ml F*l'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*O  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*y % +43 h % F*t% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*{% h % ."MFBPCR"\AS.seek 0.96ml F*['0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %0 ."MFBPCR"\AS.seek 0.985ml F*(' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*r% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*` I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *N'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*7  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *>' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *m' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*@'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +43 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*5% h %Z ."MFBPCR"\AS.seek 0.81ml F*P'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*- n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *I'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*8 % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )h h  C' 'd h  P% %) h  F* *q h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *Q% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)W% h % .t\SS.configure SSconfig C'T& S)#.0X 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:50:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #7) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'r& h & .tHand.open M&(1 +150 h 1 M&`& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &N'η +91 h 'η .tHand.close M&e/ +150 h / M&7& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &>(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&@1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'_& h &W C'f% h %4 .t\CSV.dial CSR C'v  +150 h  C'5% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '^'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '\\% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Y'[ +463 h '[ .t\CSV.dial :waste C'P +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C' & h &W C'% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'R' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +75 .t\CTV.dial :intake C'#  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'1  +75 h #t\Flushing upper manifold C'n& h &W C'2% h %4 .t\CSV.dial :flush C'"  +75 h  .t\CS.seek 5ml C'B'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +37 h @ C'% h %p .t\CS.seek 7ml C'G'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'% +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'D'4 +507 h '4 C'4& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'J'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +38 h @ C'6% h %0 .t\CS.seek 5ml C'9'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +507 h '0 .t\CSV.dial :waste C'V +75 h .t\CS.seek 0ml C'C'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&S/ +150 h / .t\CC.seek :open C'-( +416 h ( M&E& h &ӷ M&8% h % .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&31 +150 h 1 M&+% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:50:09CEST26-Sep-18\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:50:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*w% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'!& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&h% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(d% h %X A(). h ., A(q& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&Q& h &- M&W% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &T( +6 F*j% h %&K ."readyMFB"\ATV.dial :waste2 F*A % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *H' +160 h ( M&& h & A(b& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &&' +89 h ' A(& h &  .t\SE.seek 2.71 Pucks below Top Plate A(g') +235 h ') A(u& h &  M&& h & .t\Elbow.seek 2 M&?( +7 h ( M&}& h & A('& h &  .t\Forearm.seek 2 + 300 counts M&\ '8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &p/ +150 h / ."downElevator"\SE.seek :down M&X& A(K'X h & M&% h %8 .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.seek CC M&|(ӷ +142 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +2 h 'X ."downElevator"\SE.coast A(5 +89 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&~1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)y+ +7 h + S)l% h % C'r& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'5J +216 h '5J ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)<  +151 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S) +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'29 h 9Td +100 C'#9 h 9kd +100 C'"9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'B9 h 9d +100 C'%9 h 9d +100 C'G9 h 9d +100 C's9 h 9 d +100 C'L9 h 9 d +100 C'n9 h 96d +100 C'9 h 9Ld +100 C'49 h 9bd +100 C'9 h 9xd +100 C'k9 h 9d +100 C'D9 h 9d +100 C'z9 h 9d +100 C'69 h 9d +100 C' 9 h 9d +100 C'J9 h 9d +100 C'9 h 9d +100 C'V9 h 9(d +100 C'i9 h 9>d +100 C'99 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'/9 h 9d +100 C'U9 h 9d +100 C'S9 h 9d +100 C'<9 h 9d +100 C'9 h 9d +100 C'89 h 9  d +100 C']9 h 9 d +100 C'>9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'39 h 9 j d +100 C'E9 h 9 $d +100 C'9 h 9 (d +100 C'I9 h 9 ,d +100 C'w9 h 9 0d +100 C'h9 h 9 4d +100 C'd9 h 9 8d +100 C')9 h 9 % h % ."downElevator"\SE.seek :down A('X +83 h 'X ."downElevator"\SE.coast A(5 +17 (% h %X M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +89 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C'I' +7 h ' .t\CSV.dial between air and CTV C'w ` +18 h ` .t\AIV.dial :bypass C'h { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'd& h & .tHand.open M&)1 +2 h 1 M&q& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&+(- +166 h (- M&W& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.seek :garage M&A'η +91 h 'η .tHand.close M&!/ +150 h / M&b& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C''(W +416 h (W C'Z& h &W .tHand.open M&p1 +150 h 1 M&Q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%} +151 h P%X% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%T' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%: +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%$' +2610 h ' ."connect"\PRV.dial CBV P%\  .t\CBV.dial PRV C'g  h +75 .t\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%K  h .t\PS.seek 0ml P%*' +2610 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%-' +2609 h ' .t\PSV.dial :mixloop P%l +38 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%O' +2610 h ' .t\PSV.dial :mixloop P%` +38 h .t\PS.seek 0ml P%y' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&(' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'c( +416 h ( M&& h &ӷ M&{% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&M1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C';& h & M&_% h %\  A(f% h %X A(v. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&e& h &- M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &P( +166 h ( M&Y& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &|' +90 h ' A(5& h &  .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': A(R& h &  M&2& h & .t\Elbow.seek 2 M&#( +6 h ( M& & h & A(& h &  .t\Forearm.seek 2 + 300 counts M&0'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M& & A(s'X h & M&^% h %8 .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'C(W +416 h (W .tHand.open M&B1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'L  +150 h  .t\CSV.dial CTV C',  +19 h  .t\CS.seek 5ml C'z'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'6' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%J  +37 h  .t\PS.seek 2.5ml P%i' +2610 h ' .t\PSV.dial :mixloop P%9 +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%n +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%/  +151 h  .t\PS.seek :empty P%U' +2609 h ' .t\CBV.dial :waste1 C'S @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'8 @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'V @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'3  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'w @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'h  +151 h C'd& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&)% h %\  A(q% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (<& h &( M&%& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &]( +143 h ( M&W& h & A(j& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &@' +89 h ' A(N& h &( .t\SE.seek 17.71 Pucks below Top Plate A('* +86 h '* M&!& h & A(b& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C .t\Elbow.hold M&E, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(A- h -+* .t\SE.seek 18.04 Pucks below Top Plate A( ' +27 h ' M&u% h %C A(+& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&D& h & A(Z& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &p' +22 h ' .t\SE.seek 19.04 Pucks below Top Plate A(Q' +37 h ' M&}& h & M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&&(ӷ +142 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'g( +416 h ( A(?& h &( A(K& h &( M&$& h &ӷ M&~% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&-& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &r'C +92 h 'C .t\Elbow.hold M&`, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &O1 +150 h 1 M&F% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&a'o +21 h 'o M&7& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &o' +22 h ' A('% h % ."downElevator"\SE.seek :down A('X +74 h 'X ."downElevator"\SE.coast A(5 +26 ({% h %X M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +112 h  P%.% h % .t\PS.seek 0.499ml P%M' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%t  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%;  +38 h .t\CBV.dial AIV C'_  +38 h .t\PRV.dial between CBV and air P%f  +19 h  .t\PRV.dial :air P%v  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %e' +251 h ' .t\PRV.dial :waste2 P%\\ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb % ' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&1 +2 h 1 M&5& h & M&H% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&/ +150 h / M&G& h &- M& % h %η .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &4' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'0& h &W .tHand.open M +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  C'c& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%B @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%1  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%L  +76 h  .tdelay 2 seconds +200 P%,% h % .t\PS.seek 1.499ml P%"' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%I  +38 h  .t\PS.seek 0ml P%z' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%6 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%J  +38 h  .tdelay 2 seconds +200 P%y% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %9'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%n% h %r .t\PS.seek 0.475ml P%/' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%i  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%S'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%8  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%V @ +75 h @ .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%3  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%w +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%h' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%d  +75 h  .t\PS.seek 2.5ml P%)' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%q +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%<% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %%' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%W% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %j'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'N { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%!  +37 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :PML P%A +37 h .t\PS.seek 0ml P%u' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '+& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'D& ."MFBPCR"\ASV.dial :SPEdelivery F*Z @@T h &W F*k  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%} +21 h  ."MFBPCR"delay 20 seconds +91 h P%X% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%@' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*] I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%T  +38 h  .t\PS.seek 0ml P%:' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%p +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%E' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +22 h ' .t\PSV.dial PSR P% +21 h  F*C% h %6 ."MFBPCR"\AS.seek 0.329ml F*?'ͯ +92 h P%K% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%$' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*~ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *-'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*&  +21 h  F*O% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *F' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*'% h % ."MFBPCR"\AS.seek 0.469ml F**' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%l +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%{' +2610 h ' .t\PSV.dial PSR P%( +113 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%Q  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*.9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*o% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%;' +2609 h ' .t\PSV.dial PSR P%_ +113 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%v' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%M' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%\\ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%e' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %5'k +2679 h 'k .t\PSV.dial :waste1 P%H  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %R' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*2 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *`'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%G' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %m' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %|' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *^'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*g I +43 h I F*0% h %Q ."MFBPCR"\AS.seek 0.01ml F*#' +171 h ' .t\PSV.dial :mixloop P%= +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %c' +619 h ' ."MFBPCR"\ATV.dial :waste F*B n +128 h n ."MFBPCR"\AS.seek :empty F*4'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*1  +86 h  F*L% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *,' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*"% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *I'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'6% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'y' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'z  '  +38 h  +113  .t\CBV.dial :waste1 C'n @ +38 h @ .t\CS.seek 0ml C'9'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*i  +22 h ."MFBPCR"delay 3 seconds +300 F*/% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'8  +37 h  C'S% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*V  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*3'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*w% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'd' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'q'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'j( +416 h ( M&!& h &ӷ M&% h % .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &b(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&}1 +150 h 1 M&X% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C']& h & M&% h %\  A(T% h %X A(x. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (@( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&:& h &- M&p% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +166 h ( M&\ & h & A(C& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(K& h &  ."WCR"\SE.seek 0.71 Pucks below Top Plate A('K +253 h 'K A(E& h &  M&~& h & ."WCR"\Elbow.seek 2 M&?( +7 h ( M&& h & A(&& h &  ."WCR"\Forearm.seek 2 + 300 counts M&O'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &$/ + h '&0 ."MFBPCR"delay 3 seconds +149 h / ."downElevator"\SE.seek :down M&& h & A(-'X M&% h %8 ."WCR"\Forearm.seek :retract M&''\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +60 ."MFBPCR"\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +39 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' ."WCR"\CC.seek :closed C'{(W +416 h (W ."WCR"Hand.open M&Q1 +13 h 'R ."MFBPCR"\ATV.dial :air2 F*a  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *.' +115 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)*+ +7 h + S)U% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C't  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F* % h % ."MFBPCR"\AS.seek 0.279ml F*w'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*3 n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*h % +43 h % F*d% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*< I +21 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F*W'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*N % +21 h % F*P% h %H ."MFBPCR"\AS.seek 0.069ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*!% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % q ."MFBPCR"\AS.seek 0.794ml F*D' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*}% h % k ."MFBPCR"\AS.seek 0.779ml F*X'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*A I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *]' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*T'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*x % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*k% h % ."MFBPCR"\AS.seek 0.579ml F*\ ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %  ."MFBPCR"\AS.seek 0.604ml F*b'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*E% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*~' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*&  +43 h  ."MFBPCR"\AS.seek 0ml F*$'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*F' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*7 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*(% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*? I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*'' +625 h ' ."MFBPCR"\ATV.dial :air3 F*- % +21 h % F*j% h % ."MFBPCR"\AS.seek 0.13ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*U' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h % ."MFBPCR"\AS.seek 0.255ml F*t'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*@% h %X ."MFBPCR"\AS.seek 0.24ml F**' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*f n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*_  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *v'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*\\'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*H  +43 h  #"MFBPCR"\Swish #1 of 2 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *2'\  +1032 h '\  F*Y% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*e% h %(A ."MFBPCR"\AS.seek 0.6ml F*G'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*m'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *R' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3472 h 'V ."MFBPCR"\SPE.coast F*g  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0417h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*0  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*^% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*#  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*,  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*6% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*n % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +107 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*3  +65 h ."MFBPCR"\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*h% h % \\ ."MFBPCR"\AS.seek 0.326ml F*d'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226215 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'P @ +76 h @ +50 S)W% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'!% h % ."WCR"\CSV.dial between air and CTV C's ` +18 h ` ."WCR"\CS.seek 7.288ml C'%' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'+  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ')'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'}  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'D' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'A  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'X  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'x' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'] @ +75 h @ +50 S)T% h % ."WCR"\CBV.dial between CSV and waste2b C'p  +94 h  C'k% h % ."WCR"\CSV.dial between air and CTV C': ` +56 h ` ."WCR"\CS.seek 7.288ml C'C' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'K  '\  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'u'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'E  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'&  +38 h  C'~% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'$' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'7% h % ."WCR"\CSV.dial between air and CTV C'( ` +57 h ` ."WCR"\CS.seek 7.288ml C'F' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'?  'O  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '-' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'j  +150 h C''& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&Q% h %\  A(% h %X A(a. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (l& h &( M&U& h &ӷ M&r% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &@( +142 h ( M&t& h & A(f& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +89 h ' A(*& h &( ."WCR"\SE.seek 18.71 Pucks below Top Plate A(' +77 h ' M&;& h & A(v& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +26 h 'C ."WCR"\Elbow.hold M&M, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 19.04 Pucks below Top Plate A(H' +27 h ' M&5% h %C A(\\& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&Y'o +20 h 'o M&2& h & A(e& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 20.04 Pucks below Top Plate A( ' +37 h ' M&G& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +89 h '\  ."WCR"\Elbow.seek CC M& (ӷ +143 h (ӷ M&R& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ ."WCR"\Forearm.seek CC M&0' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'^( +415 h ( A(`& h &( A(& h &( M&.& h &ӷ M&B% h % ."WCR"\Forearm.seek :retract M&1'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &#( +143 h ( M& & h & A("& h &( ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.seek 1 M&L'C +92 h 'C ."WCR"\Elbow.hold M&,, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &61 +150 h 1 M&4% h %C A(J& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&z'o +20 h 'o M&& h & A(n& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' A(% h % ."downElevator"\SE.seek :down A(/'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (8% h %X M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +90 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'9& h & .tHand.open M&S1 +2 h 1 M&>& h & M&V% h %\  .t\Elbow.seek FlushPuck::Garage M&I(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&w'η +91 h 'η .tHand.close M&h/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'W(W +416 h (W C'& h &W .tHand.open M&!1 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'+% h % .t\CSV.dial CSR C'%  +37 h  C'}% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'A% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'X'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'x& h &W C']% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C':% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'\  .t\CTV.dial CSV C'K  +38 h  +112  .t\CBV.dial :waste1 C'C @ +75 h @ .t\CS.seek 0ml C'E'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'u& h &W C'% h %4 .t\CSV.dial :flush C'&  +75 h  .t\CS.seek 5ml C'~'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +38 h @ C'$% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing upper manifold C'(& h &W C'% h %4 .t\CSV.dial :flush C'O  +75 h  .t\CS.seek 5ml C'?'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' 'j  +38 h +75 .t\CTV.dial :air C'Q  +151 h .t\CS.seek 0ml C''4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'l +38 h .t\CS.seek 0ml C'U'4 +506 h '4 C'r& h &W C't% h %4 .t\CSV.dial :flush C'f  +38 h  .t\CS.seek 3ml C'*'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C';% h %0 .t\CS.seek 5ml C'v'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +507 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'2'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'e& h &W C'o% h %4 .t\CSV.dial CSR C'G  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'_'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'R' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'g  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Y'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'H  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'M'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'm  '{  +38 h  +113  .t\CBV.dial :waste1 C'` @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'.  +37 h  C'B% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'0  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'6'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'4  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +709 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'1  +38 h +75 .t\CTV.dial :air C'#  +151 h .t\CS.seek 0ml C'8'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'9 +38 h .t\CS.seek 0ml C'S'4 +506 h '4 #t\Flushing upper manifold C'>& h &W C'V% h %4 .t\CSV.dial :flush C'L  +38 h  .t\CS.seek 5ml C'z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'3'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'/ +113 h .t\CTV.dial :air .\CS.seek 0ml C'y'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C', +38 h .t\CS.seek 0ml C'h'4 +507 h '4 C' & h &W C'% h %4 .t\CSV.dial :flush C'i  +37 h  .t\CS.seek 3ml C'w'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'q% h %0 .t\CS.seek 5ml C'I'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'<'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'!'4 +506 h '4 #t\Flushing lower manifold's waste1 port C's& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 5ml C'%'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'P% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'A  ')  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'X'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'x& h &W C']% h %4 .t\CSV.dial :flush C'T  +75 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C':% h %p .t\CS.seek 7ml C'p'i +416 h 'i ."connect"\CSV.dial CTV C'\  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'K  +75 h  .t\CS.seek 0ml C'C'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'u% h %4 .t\CSV.dial CSR C'  +38 h  C'&% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'E'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'b' +416 h ' ."connect"\CSV.dial CBV C'$ .t\CBV.dial CSV C'~  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '('4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'O% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'F' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'?'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C'a( +415 h ( M&r& h &ӷ M&t% h % .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &U(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&v1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&e1 +2 h 1 M&o& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &G(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&@% h %η .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +264 h (ӷ M&H& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'm& h &W .tHand.open M&{1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'.& h &W C'B% h %4 .t\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'0'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '"'[ +463 h '[ .t\CSV.dial :waste C'4 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +2010 h '4 #t\Purging external Intake C'6& h &W C'% h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# +75 h .t\CTV.dial :intake C'n  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'8'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'9  +76 h #t\Flushing upper manifold C'& h &W C'>% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z +113 h .t\CTV.dial :air .\CS.seek 0ml C'/'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'3'0 +506 h '0 .t\CSV.dial :waste C'y +38 h .t\CS.seek 0ml C','4 +507 h '4 C'& h &W C' % h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'i'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'w'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +506 h '0 .t\CSV.dial :waste C'< +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&^& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&!' +91 h ' .tHand.close M&}/ +150 h / .t\CC.seek :open C'P( +416 h ( M&%& h &ӷ M&A% h % .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &]'η +91 h 'η .tHand.open M&T1 +150 h 1 M&X% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST05-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:51:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST05-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0417h25ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*\  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*&'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*l % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*;'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*f  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0419h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ARV.dial :carrier F*H  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*a n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +21 h ."MFBPCR"delay 2 seconds +200 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*Y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*"  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*#'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*9'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*V' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*L'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0419h25ml.pcr F*/% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*3  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +21 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*}% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*x  +31 h  ."MFBPCR"\ATV.dial :air3 F*s % +87 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +21 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0421h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*O  +10 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*l  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*r n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*v % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*f'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*5% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*o n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*U  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0421h25ml.pcr F*G% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*g  +22 h ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *.'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +21 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*6  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +65 h  F*1% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*9  +31 h  ."MFBPCR"\ATV.dial :air3 F*8 % +87 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*z% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*L'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V @CEST1538690416.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *+'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0500h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ARV.dial :carrier F*^  +11 h  F*R% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*x  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*s  +22 h ."MFBPCR"delay 2 seconds +200 F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*) I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*]% h %Fj ."MFBPCR"\AS.seek 0.524ml F*X' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*: n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*Z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*K  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*\ '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*u  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*&  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*O  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0500h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*F  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *l'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*Q% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +64 h  F*'% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*m% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0502h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ARV.dial :carrier F*"  +11 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*9  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*V I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*3  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*L'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*y  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*i'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*I  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*}'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*R % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0502h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*+ @@T *=  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +151 h F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *x' +423 h ' ."MFBPCR"\ATV.dial :ASV F*s n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*T  +43 h  F*!% h % ."MFBPCR"\AS.seek 0.62ml F*)'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*] n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *-'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *:'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*d'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *p' +423 h ' ."MFBPCR"\ATV.dial :air3 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*u' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +21 h F*\ % h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*&' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*~' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*O n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*b% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*j% h % ."MFBPCR"\AS.seek 0.579ml F*7' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %  ."MFBPCR"\AS.seek 0.604ml F*l'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*N% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*t  +22 h  ."MFBPCR"\AS.seek 0ml F*W'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*' % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*f% h % ."MFBPCR"\AS.seek 0.58ml F*o' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +21 h F*@% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F**' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*_ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*U'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *G'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *g' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*m n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *H'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*a n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +202 h 'R ."MFBPCR"\SPE.coast F*B  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*{'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*. n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*0 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*6  +43 h  F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *"': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*J  +64 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*n% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *#' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*9 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *z': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*>  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b */'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*3% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *y'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *L': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*h n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*, n +151 h n ."MFBPCR"\AS.seek 0ml F*q'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*i % +43 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h % ."MFBPCR"\AS.seek 0.96ml F*^'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %0 ."MFBPCR"\AS.seek 0.985ml F*}' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*A% h % ."MFBPCR"\AS.seek 0.97ml F*%'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*s I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *!'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *]'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *-'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*:  +43 h  F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *K' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *d' +9988 h ' ."MFBPCR"\ATV.dial AIV F*Z I +43 h I ."MFBPCR"\AS.seek 0.02ml F*p' +9880 h ' ."MFBPCR"\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.seek 0ml F*\ 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*u% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*b% h %n ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +64 h  F*F% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*t  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *v' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*2  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*B n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *H'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*` % +108 h % ."MFBPCR"\AS.seek 0ml F*.'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast + C'5 M&6 P%5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )Y h  C' ' h  P% %{ h  F* *0 h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ P% *\ F*\ 9\ P% 0\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *?% h %V .t\AS.configure ASconfig S)6% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'1& S)#.0X  M&% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &z'\ h ."rdySampler"\CSV.dial :waste1 C' h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)3 h  S)/  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M&/ .t\Elbow.jog 1500 M& * +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'y'4 S)#.0X 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:52:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #8) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&}1 +150 h 1 M&s& h &M M&%% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &T(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &]'η +91 h 'η .tHand.close M&)/ +150 h / M&:& h &- M&X% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +264 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &D' +91 h ' .t\CC.seek :closed C'u(W +416 h (W C'p& h &W .tHand.open M&\ 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'$& h &W C'C% h %4 .t\CSV.dial CSR C'&  +150 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'E'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '~% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'O'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'b& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( 'F  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'Q'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'7  +75 h #t\Flushing upper manifold C'r& h &W C'N% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'W% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C'f'4 +507 h '4 C';& h &W C'% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'o% h %0 .t\CS.seek 5ml C'@'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'*'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.seek CC M&m' +91 h ' .tHand.close M&g/ +150 h / .t\CC.seek :open C'B( +416 h ( M&a& h &ӷ M&`% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &c'η +91 h 'η .tHand.open M&1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 16:53:00CEST26-Sep-18\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:52:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*P% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'+& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&Y% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A({. h ., A(0& h &  .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&?& h &- M&6% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &1( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*J % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +160 h ( M&"& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &n' +89 h ' A(& h &  .t\SE.seek -0.29 Pucks below Top Plate A(9'\\ +265 h '\\ A(#& h &  M&8& h & .t\Elbow.seek 2 M&( +6 h ( M&V& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&>'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&S& A(3'X h & M&/% h %8 .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.seek CC M&,(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'R(W +416 h (W .tHand.open M&=1 +150 h 1 M&z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)i+ +6 h + S)q% h % C'A& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +75 h  C'% h %4 #t\Pulling 1ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1ml C'#P d@b '' +350 h ' .tdelay 5 seconds +500 .\CSR.select :air C' +6 '3% h % .t\CS.seek 6ml C'B' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'= +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b 'z' +710 h ' .t\CSV.dial CSR C'q  +150 h  C'A% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'w' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 'h% h %  .t\CS.seek 4.5ml C''` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'i  +38 h  .t\CS.seek 1.275ml C'%' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C's   '9 h 9d +100 C'!9 h 9d +100 C'x9 h 91d +100 C'}9 h 9J d +100 C'49 h 9bd +100 C':9 h 9zd +100 C'X9 h 9d +100 C'<9 h 9d +100 C'I9 h 9 d +100 C'Z9 h 9$d +100 C'd9 h 9(d +100 C'T9 h 9 ,d +100 C'-9 h 9$0d +100 C'M9 h 9<4d +100 C']9 h 9S8d +100 C'9 h 9kd +100 C'N9 h 9Td +100 C'9 h 9kd +100 C'l9 h 9d +100 C'Q9 h 9d +100 C'W9 h 9d +100 C't9 h 9d +100 C''9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'r9 h 96d +100 C'v9 h 9Ld +100 C';9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'f9 h 9d +100 C'29 h 9d +100 C'o9 h 9d +100 C'e9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'U9 h 9>d +100 C'@9 h 9Td +100 C'G9 h 9id +100 C'59 h 9d +100 C'9 h 9d +100 C'_9 h 9d +100 C'g9 h 9d +100 C'9 h 9d +100 C'u9 h 9d +100 C'`9 h 9  d +100 C'k9 h 9 d +100 C'K9 h 9 *d +100 C'm9 h 9 ?d +100 C'[9 h 9 Td +100 C'9 h 9 j d +100 C'a9 h 9 $d +100 C'\\9 h 9 (d +100 C'H9 h 9 ,d +100 C'P9 h 9 0d +100 C'Y9 h 9 4d +100 C'9 h 9 8d +100 C'{9 h 9 9 h 9 1d +100 C'9 h 9 Dd +100 C'39 h 9 Xd +100 C'9 h 9 kd +100 C'=9 h 9 ~d +100 C'B9 h 9 d +100 C'q9 h 9 d +100 C'A9 h 9 d .t\CH.hold 5:00,85C C'z   P h  +30000  C'h9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'w'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'i @ +113 h @ .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'!  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'x @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'}  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'4 @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C':'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'X +112 h .t\CBV.dial CSV C'<  +19 h C'I% h %4 .t\CS.seek 5ml C'Z'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'T% h %p .t\CS.seek 7ml C'-'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'M @ +37 h @ .t\CS.seek 10ml C']' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C' ` +132 h ` #tLysing Complete C'$& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&C% h %\  A(&% h %X A(p. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (\ (( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &( M&)& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &E( +143 h ( M&& h & A(O& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &b' +89 h ' A(& h &( .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' M& & h & A(F& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &('C +25 h 'C .t\Elbow.hold M&7, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(j- h -+ .t\SE.seek 20.04 Pucks below Top Plate A(*' +27 h ' M&N% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&l'o +21 h 'o M&Q& h & A(W& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' .t\SE.seek 21.04 Pucks below Top Plate A('' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&r(ӷ +142 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'f( +416 h ( A(2& h &( A(o& h &( M&e& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&U& h & A(@& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &G'C +93 h 'C .t\Elbow.hold M&5, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&_% h %C A(g& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&u& h & A(`& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &k' +22 h ' A(K% h % ."downElevator"\SE.seek :down A(m'X +53 h 'X ."downElevator"\SE.coast A(5 +47 ([% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'\\ @ +19 h @ .t\CS.seek 10ml C'H' +7 h ' .t\CSV.dial between air and CTV C'P ` +18 h ` .t\AIV.dial :bypass C'Y { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&{1 +2 h 1 M&0& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&.(- +166 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&J'η +91 h 'η .tHand.close M&+/ +150 h / M&& h &- M&c% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'|& h &W .tHand.open M& 1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%V +151 h P%S% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%n  +37 h  .t\PS.seek 0ml P%/' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%9  C'>  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%3  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%=  +38 h  .t\PS.seek 2.5ml P%B' +2609 h ' .t\PSV.dial :mixloop P%q +38 h .t\PS.seek 0ml P%A' +2610 h ' .t\PSV.dial :air P%z  +37 h  .t\PS.seek 2.5ml P%h' +2610 h ' .t\PSV.dial :mixloop P%% +38 h .t\PS.seek 0ml P%i' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +7 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&}' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'( +416 h ( M&X& h &ӷ M&<% h % .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Z'η +91 h 'η .tHand.open M&M1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&$% h %\  A(C% h %X A(&. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (p( +357 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (D& h & M&)& h &- M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&O& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(F& h & .t\SE.seek 20.71 Pucks below Top Plate A(,' +56 h ' A(j& h & M&N& h & .t\Elbow.seek 3 M&( +7 h ( M&Q& h & A(W& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(L'X M&b& h & M&E% h %B .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&((ӷ +143 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'*(W +416 h (W .tHand.open M&t1 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'R  +19 h  .t\CS.seek 5ml C'2'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'o' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'e'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%U' +2610 h ' .t\PSV.dial :mixloop P%@ +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%_' +2610 h ' .t\CBV.dial :waste1 C'g @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'u  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'` @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'K  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +18 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'[' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'\\'4 +2647 h '4 .t\CBV.dial :waste1 C'P @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'Y  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&{% h %\  A(0% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (G( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b M&'& A(& h &  &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(m'Z .t\Elbow.seek CC M&$&.,(\ #b &k(ӷ +6 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&\ ' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'5( +23 h 'Z +393 ( A(+& h &  A(& h &  M&c& h &ӷ M&a% h % .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&#& h & A(.& h &  .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 2 M&' +92 h '  .t\Elbow.hold M&f, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &|1 +150 h 1 M& % h %  A(?& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&V'8 +21 h '8 M&S& h & A(8& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A(1% h %Z ."downElevator"\SE.seek :down A('X +100 (/% h % M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%3% h % .t\PS.seek 0.499ml P% ' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%= +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%q  +37 h .t\CBV.dial AIV C'B  +38 h .t\PRV.dial between CBV and air P%z  +19 h  .t\PRV.dial :air P%A  +19 h  P%%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %h' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %!' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'x& h & .tHand.open M&41 +2 h 1 M&"& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.seek :garage M&}'η +91 h 'η .tHand.close M&d/ +150 h / M&T& h &- M&w% h %η .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +264 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'&& h &W .tHand.open M&Z1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  C'~& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%O @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%F  +75 h  .tdelay 2 seconds +200 P%:% h % .t\PS.seek 1.499ml P%j' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%N' +1094 h ' .t\PSV.dial :air P%Q  +38 h  .t\PS.seek 0ml P%W' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%- +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%b% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %E'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P% % h %r .t\PS.seek 0.475ml P%v' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%;  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%,  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%7  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%R +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%l' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%p  +76 h  .t\PS.seek 2.5ml P%H' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%2 +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%o  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %i' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'@ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %r'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %U' +1313 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 2.5ml P%u' +2610 h ' .t\PSV.dial :PML P%` +38 h .t\PS.seek 0ml P%K' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*e @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%[ +21 h  ."MFBPCR"delay 20 seconds +92 h P%P% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*{% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *0'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%' +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%6' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%]' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*(  +22 h ' .t\PSV.dial PSR P% +21 h  F**% h %6 ."MFBPCR"\AS.seek 0.329ml F*+'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%c' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*a % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%.  +37 h  .t\PS.seek 0ml P%\ ' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*k  +22 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b * ' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%? +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%S' +217 ."MFBPCR"\ATV.dial :AIV F*8 I +43 h I F*1% h % ."MFBPCR"\AS.seek 0.469ml F*J' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P%9 +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%V' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*39 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*n n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*G'% +336 h ' .t\PSV.dial PSR P%_ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%=' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial PSR P%B +113 h P%z% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%h' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %"'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %X' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*< I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *f'V +850 h ' .t\PSV.dial :air P%d  +113 h  .t\PS.seek 2.5ml P%T' +2609 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %>' +5106 h ' .t\PSV.dial :air P%}  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %I' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *M'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*5 I +43 h I F*&% h %Q ."MFBPCR"\AS.seek 0.01ml F*Z' +171 h ' .t\PSV.dial :mixloop P%D +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %~' +619 h ' ."MFBPCR"\ATV.dial :waste F*O n +129 h n ."MFBPCR"\AS.seek :empty F*$'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*F% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *:' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*!  +42 h F*j% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *Q'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'N  +38 h  C'-% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'b' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'E'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*;  +22 h ."MFBPCR"delay 3 seconds +300 F*v% h %_ ."MFBPCR"\AS.seek 0.434ml F*,'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*7'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*t n +108 h n F*R% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'p' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'2'4 +2647 h '4 C'o& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'r( +416 h ( M&g& h &ӷ M&U% h % .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&[1 +150 h 1 M&P% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'& h & M&{% h %\  A( % h %X A(y. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Y( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ('& h & M&& h &- M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &6( +166 h ( M&(& h & A(*& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h & ."WCR"\SE.seek 19.71 Pucks below Top Plate A(0' +66 h ' A(]& h & M&a& h & ."WCR"\Elbow.seek 3 M&+( +6 h ( M&.& h & A(k& h & ."WCR"\Forearm.seek 3 + 300 counts M&|'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down A(#'X M&?& h & M&8% h %B ."WCR"\Forearm.seek :retract M&1'\ +39 h '&0 ."MFBPCR"delay 3 seconds +27 h 'X ."downElevator"\SE.coast A(5 +25 h '\  ."WCR"\Elbow.seek CC M&\ (ӷ +143 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' ."WCR"\CC.seek :closed C'9(W +14 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +359 h (W ."WCR"Hand.open M&m1 +150 h 1 M&V% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +50 h 'R ."MFBPCR"\ATV.dial :air2 F*n  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +19 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)J+ +6 h + S)_% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  ."WCR"\CBV.dial SSV +76 h  #"WCR"\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *X' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*w % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +3472 h 'V ."MFBPCR"\SPE.coast F*5  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0517h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*&  +64 h  ."MFBPCR"\ARV.dial :carrier F*D  +11 h  F*M% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*!  +22 h ."MFBPCR"delay 2 seconds +200 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*:  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*-% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*W% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*; n +22 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*Q'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*,  +43 h  ."MFBPCR"\ASV.dial :waste1 F*7  +65 h ."MFBPCR"\ARV.dial :air1 F*R  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226027 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'@  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'o @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C's @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'C  +94 h  C'g% h % ."WCR"\CSV.dial between air and CTV C') ` +19 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'U  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'H'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'[  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'`  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'P  +38 h  C'{% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'y' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'*' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '(  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ']'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'a  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'K  +37 h  C'.% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'c' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'6 @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'8  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C' ' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'#  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '+'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'0  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'1& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&V% h %\  A(n% h %X A(m. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&_& h &ӷ M&/% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +143 h ( M&S& h & A(z& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &B' +89 h ' A(J& h &  ."WCR"\SE.seek -0.29 Pucks below Top Plate A(%'\\ +265 h '\\ M&q& h & A(A& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &G' +25 h '  ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow A(4- M&$&.,(\ #b h -+\\ ."WCR"\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&"% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&x'8 +20 h '8 M&<& h & A(h& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &d' +23 h ' ."WCR"\SE.seek 1.04 Pucks below Top Plate A(}' +36 h ' M&T& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +89 h '\  ."WCR"\Elbow.seek CC M&w(ӷ +143 h (ӷ M&X& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ ."WCR"\Forearm.seek CC M&&' +91 h ' ."WCR"Hand.close M&D/ +150 h / ."WCR"\CC.seek :open C'M( +416 h ( A(f& h &  A(& h &  M&& h &ӷ M&O% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Z( +142 h ( M&!& h & A(j& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &F' +93 h '  ."WCR"\Elbow.hold M&:, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &-1 +150 h 1 M&$% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&W'8 +20 h '8 M&N& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &b' +23 h ' A(\\% h % ."downElevator"\SE.seek :down A(v'X +100 (L% h %S M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'E& h & .tHand.open M&1 + A(6 + h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.seek :garage M&R'η +91 h 'η .tHand.close M&l/ +150 h / M&t& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +264 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'C& h &W .tHand.open M&g1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'U& h &W C'% h % .t\CSV.dial CSR C'i  +38 h  C'[% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '`% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'P'[ +463 h '[ .t\CSV.dial :waste C'{ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'y& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '(  +38 h  +113  .t\CBV.dial :waste1 C'* @ +75 h @ .t\CS.seek 0ml C'a'4 +766 h '4 #t\Flushing lower manifold's waste2b port C']& h &W C'k% h %4 .t\CSV.dial :flush C'K  +75 h  .t\CS.seek 5ml C'.'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'c% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'8  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'|& h &W C'?% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'#'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'0% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '1  +38 h +75 .t\CTV.dial :air C'V  +151 h .t\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'm'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'_'4 +506 h '4 C'/& h &W C'S% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'J'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'3% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'A'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +507 h '0 .t\CSV.dial :waste C'" +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'<& h &W C'h% h %4 .t\CSV.dial CSR C'T  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'B'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'Y' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'% +113 h .t\CTV.dial :intake C'G  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'X' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'5  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'x'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'd% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'9  '>  +38 h  +113  .t\CBV.dial :waste1 C'f @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'O% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  +38 h  .t\CBV.dial :waste2b C'w  +75 h  .t\CS.seek 0ml C'-'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'$  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'N'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ '  +37 h +75 .t\CTV.dial :air C'Z  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'E +38 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'F  +37 h  .t\CS.seek 5ml C'W'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'b% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CBV C'7 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'v'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C': +37 h .t\CS.seek 0ml C'l'4 +507 h '4 C't& h &W C'% h %4 .t\CSV.dial :flush C';  +37 h  .t\CS.seek 3ml C'R'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +38 h @ C'2% h %0 .t\CS.seek 5ml C'Q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'C +76 h .t\CS.seek 0ml C'g'4 +506 h '4 #t\Flushing lower manifold's waste1 port C')& h &W C'U% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C's% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  'H  +37 h  +113  .t\CBV.dial :waste1 C'{ @ +38 h @ .t\CS.seek 0ml C'P'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'y& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'(  +75 h  .t\CS.seek 0ml C'*'4 +766 h '4 #tDisinfecting external Intake with kill C''& h &W C']% h %4 .t\CSV.dial CSR C'k  +38 h  C'K% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'a'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'u' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '.  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'8' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'6  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '|'4 +607 h '4 .t\CSV.dial CSR C'?  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C' ' +1372 h ' .t\CSV.dial CBV C'0 +113 h .t\CS.seek 0ml C'#'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&1& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'm( +416 h ( M&/& h &ӷ M&S% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &_(- +264 h (- M&\ & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &J'η +91 h 'η .tHand.open M&q1 +150 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'4& h & .tHand.open M&<1 +2 h 1 M&h& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &T(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&%/ +150 h / M&G& h &- M&Y% h %η .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &x' +91 h ' .t\CC.seek :closed C' (W +415 h (W C'>& h &W .tHand.open M&91 +150 h 1 M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'O% h %4 .t\CSV.dial CSR C'  +113 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'&'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'w% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'j'[ +463 h '[ .t\CSV.dial :waste C'$ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'-& h &W C'N% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'E  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'F'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'}% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W +113 h .t\CTV.dial :air .\CS.seek 0ml C'7'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +507 h '0 .t\CSV.dial :waste C'v +37 h .t\CS.seek 0ml C':'4 +507 h '4 C'& h &W C't% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C';'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'o% h %0 .t\CS.seek 5ml C'2'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'R'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'C'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&M& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C's( +416 h ( M&i& h &ӷ M&`% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &{(- +264 h (- M&r& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&P% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST06-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:54:43CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST06-Oct-18" +263628 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0517h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*( % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *''Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +21 h F*k% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*6  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*8  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*S'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0519h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*T'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*!  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*#' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*w  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*$' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*j  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.536ml F*N'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*Z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*}% h % \\ ."MFBPCR"\AS.seek 0.326ml F*F'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0519h25ml.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*U  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *X'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +32 h  ."MFBPCR"\ATV.dial :air3 F*) % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*e'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0521h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*|% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +22 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*/ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*n' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*\ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*q % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*_  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*B% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0521h25ml.pcr F*T% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*5  +21 h ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*9% h %Z ."MFBPCR"\AS.seek 0.81ml F*!'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*#% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*-  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +31 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *}' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*W% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +21 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*F'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V @CEST1538776809.69 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0600h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ARV.dial :carrier F*M  +10 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*D'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *`'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*)  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*H I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*P' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*e' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*(  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*' n +21 h n ."MFBPCR"delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*K  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*.  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*8% h % \\ ."MFBPCR"\AS.seek 0.326ml F*u'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0600h25ml.pcr F*?% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*+'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*%  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +21 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *O'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0602h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*N'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\\ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*W% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*= % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*,  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*F'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +22 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*:'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*l n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*R  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*['{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*X % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0602h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*D  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *y' +424 h ' ."MFBPCR"\ATV.dial :ASV F*) n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*g% h % ."MFBPCR"\AS.seek 0.62ml F*H'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*p'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*P % +43 h % F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :air3 F*e% h % ."MFBPCR"\AS.seek 0.53ml F*]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*'' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h % H ."MFBPCR"\AS.seek 0.735ml F*K' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*a% h % Ű ."MFBPCR"\AS.seek 0.72ml F*.' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*6 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*8% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*? % +43 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*1% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +21 h % ."MFBPCR"delay 3 seconds +300 F*V% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*+' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*S  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*n% h %V ."MFBPCR"\AS.seek 0.01ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*z% h % ."MFBPCR"\AS.seek 0.58ml F*h' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*<% h % q ."MFBPCR"\AS.seek 0.605ml F*%'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*Y% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*J' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*B n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*G  +21 h  ."MFBPCR"\AS.seek 0ml F*_'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *T'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*d  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *5' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*> n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*m n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +202 h 'R ."MFBPCR"\SPE.coast F*O  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*9'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*x  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *!' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*& I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*-  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *j': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*$% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *N'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*\\ n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Z' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*E I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *W': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*=% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *7'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*} n +65 h n F*,% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*b I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*t  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *F': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*l n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*: n +150 h n ."MFBPCR"\AS.seek 0ml F*2'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*o  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*; % +43 h % F*Q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*M'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %0 ."MFBPCR"\AS.seek 0.985ml F*[' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*`% h % ."MFBPCR"\AS.seek 0.97ml F*i'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*) I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *g'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *r'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*P% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *p' +9988 h ' ."MFBPCR"\ATV.dial AIV F*e I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*]% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*k  +21 h ."MFBPCR"\ATV.dial :air3 F*' % +43 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +21 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*u% h %n ."MFBPCR"\AS.seek 0.82ml F*|'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *@'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*+ % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*n% h % ."MFBPCR"\AS.seek 0.81ml F*3'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*O n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*f % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2991358 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )x h  C' ' h  P% %9 h  F* *& h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *!  h  .t\SPE.configure SPEconfig F*\ 2 *#% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)-% h % .t\SS.configure SSconfig C'& S)#.0X 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:55:43CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #9) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&[1 +150 h 1 M&)& h &M M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&H/ +150 h / M&& h &- M&P% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +264 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'](W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'c& h &W C'*% h %4 .t\CSV.dial CSR C'K  +150 h  C'6% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'a'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '.% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'8 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '?'4 +2009 h '4 #t\Purging external Intake C'u& h &W C'% h %4 .t\CSV.dial :flush C'0  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '|  h +75 .t\CTV.dial :intake C'@  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'V'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'/& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'S'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +37 h @ C'% h %p .t\CS.seek 7ml C'\ 'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'4 +37 h .t\CS.seek 0ml C'z'4 +507 h '4 C'3& h &W C'% h %4 .t\CSV.dial :flush C'<  +38 h  .t\CS.seek 3ml C'A'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'h% h %0 .t\CS.seek 5ml C'Y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'%'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +507 h '0 .t\CSV.dial :waste C'G +75 h .t\CS.seek 0ml C'J'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&_& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +7 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C'O( +416 h ( M&m& h &ӷ M&f% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &{(- +264 h (- M&~& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&^1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:55:49CEST26-Sep-18\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:55:43CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&x% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(9. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (&( +6 F*!% h %&K ."readyMFB"\ATV.dial :waste2 F*# % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *-' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&w& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &$( +166 h ( M&j& h & A(\\& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(N& h & .t\SE.seek 18.71 Pucks below Top Plate A(E' +77 h ' A(Z& h & M&L& h & .t\Elbow.seek 3 M&( +6 h ( M&& h & A(& h & .t\Forearm.seek 3 + 300 counts M&='B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &}/ +150 h / ."downElevator"\SE.seek :down M&,& A('X h & M&7% h %B .t\Forearm.seek :retract M&'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&:(ӷ +143 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &R' +91 h ' .t\CC.seek :closed C'X(W +416 h (W .tHand.open M&D1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S);+ +6 h + S)2% h % C'`& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)I  +73 h ."airStroke"\CS.seek 0ml C'"'4 +7 h '4 ."airStroke"\CSV.dial :air C'^ @ +70 h  .t\CTV.dial :air .delay 15 seconds +5 h @ ."airStroke"\CS.seek 10ml C's' +910 h ' +585 .t\SSV.dial :spit,avoiding: :exhaust S) +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'  .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'@9 h 9kd +100 C'9 h 9d +100 C'V9 h 9d +100 C'9 h 9d +100 C'S9 h 9d +100 C'9 h 9d +100 C'\ 9 h 9d +100 C'n9 h 9 d +100 C'?9 h 9 d +100 C'/9 h 96d +100 C'q9 h 9Ld +100 C'39 h 9bd +100 C'9 h 9xd +100 C'+9 h 9d +100 C'z9 h 9d +100 C'9 h 9d +100 C'h9 h 9d +100 C'<9 h 9d +100 C'A9 h 9d +100 C'%9 h 9d +100 C'G9 h 9(d +100 C'_9 h 9>d +100 C'Y9 h 9Td +100 C'T9 h 9id +100 C'49 h 9d +100 C'd9 h 9d +100 C'B9 h 9d +100 C'59 h 9d +100 C''9 h 9d +100 C']9 h 9d +100 C'f9 h 9  d +100 C'>9 h 9 d +100 C'(9 h 9 *d +100 C'~9 h 9 ?d +100 C'I9 h 9 Td +100 C'^9 h 9 j d +100 C'm9 h 9 $d +100 C'"9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C'x9 h 9 4d +100 C'9 h 9 8d +100 C'99 h 9 ' +22 h ' A((% h % ."downElevator"\SE.seek :down A(~'X +100 (I% h %B M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'" @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C'x { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&91 +2 h 1 M&!& h & M&#% h %\  .t\Elbow.seek FlushPuck::Garage M&s(- +166 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&j& h &- M&\\% h %η .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +265 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'Z& h &W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%7% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%t' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%E  C'=  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%D  +38 h  .t\PS.seek 2.5ml P%O' +2609 h ' .t\PSV.dial :mixloop P%2 +38 h .t\PS.seek 0ml P%`' +2610 h ' .t\PSV.dial :air P%W  +37 h  .t\PS.seek 2.5ml P%l' +2610 h ' .t\PSV.dial :mixloop P%i +38 h .t\PS.seek 0ml P%;' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +7 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&[' +91 h ' .tHand.close M&-/ +150 h / .t\CC.seek :open C'b( +416 h ( M&P& h &ӷ M&% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M& 1 +150 h 1 M&)% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'k& h & M&c% h %\  A(*% h %X A(K. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( ( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&H& h &- M&.% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &8( +166 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &:' +90 h ' A(F& h & .t\SE.seek 17.71 Pucks below Top Plate A(6'* +86 h '* A(|& h & M&1& h & .t\Elbow.seek 3 M&( +6 h ( M&@& h & A(V& h & .t\Forearm.seek 3 + 300 counts M&'B +23 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &\ / +150 h / ."downElevator"\SE.seek :down M&n& A(u'X h & M&a% h %B .t\Forearm.seek :retract M&0'\ +86 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .t\Elbow.seek CC M& (ӷ +143 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'J(W +416 h (W .tHand.open M&S1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'?  +150 h  .t\CSV.dial CTV C'X  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'h' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'<'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%A  +37 h  .t\PS.seek 2.5ml P%_' +2610 h ' .t\PSV.dial :mixloop P%Y +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%/ +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%d  +151 h  .t\PS.seek :empty P%B' +2609 h ' .t\CBV.dial :waste1 C'5 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C']  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'f @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'(  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'% ` +19 h ` .t\CSV.dial :air C'G @ +37 h @ .t\CS.seek 10ml C'I' +2648 h ' .t\CSV.dial CTV C'^  +37 h  .t\CS.seek 0ml C'T'4 +2647 h '4 .t\CBV.dial :waste1 C'" @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C''  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&x% h %\  A(% h %X A(9. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (!( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (#& h &  M&& h &ӷ M&>% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &w( +143 h ( M&& h & A(~& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 1.71 Pucks below Top Plate A(4': +244 h ': M&& h & A(j& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &\\' +25 h '  .t\Elbow.hold M&m, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A({' +27 h ' M&L% h %  A(s& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&N'8 +21 h '8 M&z& h & A(Z& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(' +36 h ' M&& h & M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&E/ +150 h / .t\CC.seek :open C'=( +416 h ( A(& h &  A(& h &  M&t& h &ӷ M&D% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &2( +143 h ( M&O& h & A(W& h &  .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 2 M&`' +92 h '  .t\Elbow.hold M&i, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &l1 +150 h 1 M&&% h %  A(g& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&y'8 +21 h '8 M&-& h & A(o& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +22 h ' A(C% h % ."downElevator"\SE.seek :down A(P'X +100 (% h %1 M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +89 h '\  +43 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%R% h % .t\PS.seek 0.499ml P% ' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%) +38 h .t\PRV.dial :air P%Q  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%k  +37 h .t\CBV.dial AIV C'c  +38 h .t\PRV.dial between CBV and air P%*  +19 h  .t\PRV.dial :air P%K  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %H' +251 h ' .t\PRV.dial :waste2 P%. @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %e' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&F& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&|(- +166 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&r/ +150 h / M&\ & h &- M&n% h %η .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +264 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' +91 h ' .t\CC.seek :closed C'6(W +416 h (W C'& h &W .tHand.open M&u1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  C'b& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%S @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%?  +76 h  .tdelay 2 seconds +200 P%X% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P% ' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%h +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%A  +38 h  .tdelay 2 seconds +200 P%;% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Y'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%/% h %r .t\PS.seek 0.475ml P%d' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%_  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%5'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%]  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%f  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %(' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%% +75 h .t\PTV.dial :PRV,avoiding: :puck P%G @ +75 h @ .t\PS.seek 0ml P%<' +2610 h ' .t\PSV.dial :air P%^  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%" +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%'' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%x' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%9  +75 h P%#% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'> & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %~'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb % ' +1313 h ' .t\PSV.dial :air P%+  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%j +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'L& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C's& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*z @@T .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*Z  P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%7% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*w I +129 h I F*4% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *\\'6 +66 .t\PSV.dial :waste1 P%$  +38 h  .t\PS.seek 0ml P%U' +1597 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%{' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%m' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*E  +22 h ' .t\PSV.dial PSR P% +21 h  F*J% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%t' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*D % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%W  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *&' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%g +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%-' +216 ."MFBPCR"\ATV.dial :AIV F*o I +43 h I F*C% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%[ +113 h P%`% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +739 h ' ."MFBPCR"delay 5 seconds +500 F*R9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*} n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*P% h % ."MFBPCR"\AS.seek 0.041ml F*!'% +336 h ' .t\PSV.dial PSR P%B +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%)' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%Q +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%k' +2609 h ' .t\PSV.dial PSR P%c +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%K' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%. +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%H' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %F'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %1' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *i'V +849 h ' .t\PSV.dial :air P%r  +113 h  .t\PS.seek 2.5ml P%\ ' +2610 h ' .t\PSV.dial :mixloop P%n +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %p' +5106 h ' .t\PSV.dial :air P%V  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %:' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*q  +43 h  F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *a'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*= I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*u' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %b' +619 h ' ."MFBPCR"\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.seek :empty F*3'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*?% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *X' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*e  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'h% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'A'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C';' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'/ @ +38 h @ .t\CS.seek 0ml C'Y'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*_  +22 h ."MFBPCR"delay 3 seconds +300 F*d% h %_ ."MFBPCR"\AS.seek 0.434ml F*]'ݏ +110 h '4 .t\CSV.dial CSR C'f  +38 h  C'5% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'%'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*G  +22 h F*(% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*^'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*< n +108 h n F*"% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'9& h &W #tGaraging FlushPuck in Collection Clamp M&#& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +7 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'~( +416 h ( M&+& h &ӷ M& % h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Z'η +91 h 'η .tHand.open M&1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'w& h & M&4% h %\  A($% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (N& h & M&U& h &- M&,% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &{( +166 h ( M&E& h & A(J& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h & ."WCR"\SE.seek 16.71 Pucks below Top Plate A(\\'; +95 h '; A(m& h & M&D& h & ."WCR"\Elbow.seek 3 M&( +7 h ( M&W& h & A( & h & ."WCR"\Forearm.seek 3 + 300 counts M&l'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down M&g& A(O'X h & M&o% h %B ."WCR"\Forearm.seek :retract M&C'\ +9 h '&0 ."MFBPCR"delay 3 seconds +82 h '\  ."WCR"\Elbow.seek CC M&(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +137 h (ӷ M&&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &-' +76 ."MFBPCR"\ATV.dial :waste2 F*[ % +15 h ' ."WCR"\CC.seek :closed C'`(W +28 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +388 h (W ."WCR"Hand.open M&1 +150 h 1 M&y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +21 h 'R ."MFBPCR"\ATV.dial :air2 F*}  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *P' +48 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)B% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'Q  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*8% h %H ."MFBPCR"\AS.seek 0.069ml F*0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*+% h % ."MFBPCR"\AS.seek 0.769ml F* ' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h % q ."MFBPCR"\AS.seek 0.794ml F*s' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*7'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*j I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *w' +4416 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek :empty F*$'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*v % +43 h % F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.579ml F*E' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*D% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*W' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*t'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *o'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*{ % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*&' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*2 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*l% h %  ."MFBPCR"\AS.seek 0.09ml F*\\' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*[  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*O% h % ."MFBPCR"\AS.seek 0.12ml F*C' +625 h ' ."MFBPCR"\ATV.dial :air3 F*~ % +21 h % F*I% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*}% h % ."MFBPCR"\AS.seek 0.23ml F*B' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h % ."MFBPCR"\AS.seek 0.255ml F*-'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*c  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *K'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*) @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*! I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*.'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *@'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*r'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*H% h %(A ."MFBPCR"\AS.seek 0.6ml F*\ '\  +1031 h '\  F*V% h %\  ."MFBPCR"\AS.seek 0.5ml F*p'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*q% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *1' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*n % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3472 h 'V ."MFBPCR"\SPE.coast F*=  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0617h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*|'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*e  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *3'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*X  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*A  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*T' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*/ % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*5'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 F*^  +65 h ."MFBPCR"\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*'% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226057 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'>  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'9 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'8 @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'6  +94 h  C'+% h % ."WCR"\CSV.dial between air and CTV C'0 ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'L  '  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'x'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 's' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'j  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'7  +38 h  C'4% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'v' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'w @ +75 h @ +50 S)$% h % ."WCR"\CBV.dial between CSV and waste2b C',  +94 h  C'Z% h % ."WCR"\CSV.dial between air and CTV C'U ` +57 h ` ."WCR"\CS.seek 7.288ml C'J' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'E  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'm'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'D  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'W% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C't' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'{ @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'o  +95 h  C'2% h % ."WCR"\CSV.dial between air and CTV C'l ` +56 h ` ."WCR"\CS.seek 7.288ml C'&' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'O'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '~' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +151 h C'C& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&y% h %\  A(}% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ( ( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Q& h &  M&B& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&-& h & A(*& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +90 h ' A(& h &  ."WCR"\SE.seek 2.71 Pucks below Top Plate A(') +234 h ') M&k& h & A(K& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &!' +26 h '  ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+) ."WCR"\SE.seek 3.04 Pucks below Top Plate A(' +27 h ' M&F% h %  A(.& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&@& h & A(H& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &r' +23 h ' ."WCR"\SE.seek 4.04 Pucks below Top Plate A(V' +36 h ' M&\ & h & M&q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +89 h '\  ."WCR"\Elbow.seek CC M&n(ӷ +143 h (ӷ M&1& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'a( +416 h ( A(i& h &  A(|& h &  M&P& h &ӷ M&S% h % ."WCR"\Forearm.seek :retract M&'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &u( +143 h ( M&e& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &?' +92 h '  ."WCR"\Elbow.hold M&X, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &h1 +150 h 1 M&3% h %  A(A& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M& & h & A(/& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &;' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(d'X +100 (f% h %  M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +90 h '\  +31 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&51 +2 A(6 h 1 M&(& h & M&G% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.seek :garage M&"'η +91 h 'η .tHand.close M&'/ +150 h / M&<& h &- M&%% h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &>(ӷ +264 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'6& h &W .tHand.open M&+1 +150 h 1 M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'L& h &W C' % h % .t\CSV.dial CSR C'  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'x'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '7'[ +462 h '[ .t\CSV.dial :waste C'4 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 's'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'v& h &W C'w% h %4 .t\CSV.dial :flush C'$  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ','p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'U% h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'E  +38 h  +113  .t\CBV.dial :waste1 C'J @ +75 h @ .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'm& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'W'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C't% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'o  +75 h  .t\CS.seek 0ml C'2'4 +766 h '4 #t\Flushing upper manifold C'l& h &W C'g% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'['p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'\\% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'C 'I  +38 h +75 .t\CTV.dial :air C'y  +151 h .t\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'Q +38 h .t\CS.seek 0ml C'B'4 +507 h '4 C'& h &W C'-% h %4 .t\CSV.dial :flush C'*  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'R% h %0 .t\CS.seek 5ml C'k'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'F +76 h .t\CS.seek 0ml C'.'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'@& h &W C'H% h %4 .t\CSV.dial CSR C'\  +151 h  C'q% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'c'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'1' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'=  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'r% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'p  '`  +38 h  +113  .t\CBV.dial :waste1 C'i @ +37 h @ .t\CS.seek 0ml C'|'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'P  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'n  +75 h  .t\CS.seek 0ml C'h'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'3  +37 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T '  +37 h +76 .t\CTV.dial :air C'u  +150 h .t\CS.seek 0ml C'f'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'Y +37 h .t\CS.seek 0ml C'5'4 +507 h '4 #t\Flushing upper manifold C'(& h &W C'G% h %4 .t\CSV.dial :flush C'?  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +37 h @ C']% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +113 h .t\CTV.dial :air .\CS.seek 0ml C'd'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'X +37 h .t\CS.seek 0ml C'''4 +507 h '4 C'<& h &W C'%% h %4 .t\CSV.dial :flush C'_  +38 h  .t\CS.seek 3ml C'"'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'#'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'6 +75 h .t\CS.seek 0ml C'+'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'0& h &W C'L% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'8% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'x  'j  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +37 h @ .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'v& h &W C'w% h %4 .t\CSV.dial :flush C'$  +75 h  .t\CS.seek 5ml C's'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'U% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'E  +75 h  .t\CS.seek 0ml C'J'4 +766 h '4 #tDisinfecting external Intake with kill C'N& h &W C'm% h %4 .t\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'D'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C't  'W +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'o' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'{  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +606 h '4 .t\CSV.dial CSR C'g  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'2'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'&' +1372 h ' .t\CSV.dial CBV C'\\ +113 h .t\CS.seek 0ml C'['4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&I& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +7 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.seek CC M&}' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&-% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&k1 +150 h 1 M&K% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&@1 +2 h 1 M&H& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &\ (- +7 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.seek :garage M&c'η +90 h 'η .tHand.close M&/ +150 h / M&!& h &- M&% h %η .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'p& h &W .tHand.open M&`1 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'P& h &W C'S% h %4 .t\CSV.dial CSR C'|  +113 h  C'e% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'3 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'A'4 +2009 h '4 #t\Purging external Intake C'h& h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'u .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'/  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'f'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Y  +75 h #t\Flushing upper manifold C'& h &W C'(% h %4 .t\CSV.dial :flush C'G  +75 h  .t\CS.seek 5ml C'?'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +37 h @ C'V% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C';'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'd +37 h .t\CS.seek 0ml C'X'4 +507 h '4 C' & h &W C'<% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.seek 3ml C'_'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +38 h @ C'9% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'"'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'# +75 h .t\CS.seek 0ml C'6'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&a& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'8( +416 h ( M&& h &ӷ M&x% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +264 h (- M&~& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &w'η +91 h 'η .tHand.open M&$1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST07-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:120\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:57:34CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST07-Oct-18" +263658 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0617h25ml.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *N'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*t'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*{  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +65 h  F*g% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*O  +32 h  ."MFBPCR"\ATV.dial :air3 F*Q % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*-'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*k% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F**  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0619h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*r% h %V ."MFBPCR"\AS.seek 0.5ml F*\ '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*i  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*P  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *|'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*e  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*[' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*n  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*3' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*/ n +22 h n ."MFBPCR"delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*G' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*f  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*5  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*] % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0619h25ml.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*^  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *%'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F*'% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*a'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +31 h  ."MFBPCR"\ATV.dial :air3 F*0 % +87 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*w% h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +21 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0621h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.53ml F*C' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*k % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F**'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*@'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*.'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0621h25ml.pcr F*\ % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*=  +22 h ."MFBPCR"\ATV.dial :air3 F*p % +87 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*n'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*[% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Y  +31 h  ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*5% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*?'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1538863218.96 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0700h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*a  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*v  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*0  +22 h ."MFBPCR"delay 2 seconds +200 F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *~'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*j I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*w% h %Fj ."MFBPCR"\AS.seek 0.524ml F*7' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*U n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*E  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +108 h  ."MFBPCR"delay 7 seconds +700 F*s% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*N n +21 h n ."MFBPCR"delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*o% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0700h25ml.pcr F*g% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*&  +22 h ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*-% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +64 h  F*C% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*r'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *3'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0702h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +21 h ."MFBPCR"delay 2 seconds +200 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *('Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*G I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*) % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*?'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +22 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*X'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*' n +22 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*L% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0702h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *v' +423 h ' ."MFBPCR"\ATV.dial :ASV F*0 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*$  +43 h  F*+% h % ."MFBPCR"\AS.seek 0.62ml F*j'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*w n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *~'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *U'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*7 % +43 h % F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *,' +423 h ' ."MFBPCR"\ATV.dial :air3 F*z% h % ."MFBPCR"\AS.seek 0.53ml F*m' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*N' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*D% h % Ű ."MFBPCR"\AS.seek 0.72ml F*t' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*{ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*o% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*g % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*I% h % ."MFBPCR"\AS.seek 0.579ml F*2' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %  ."MFBPCR"\AS.seek 0.604ml F*Q'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*O' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*-  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *R'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*C % +108 h % F*}% h %V ."MFBPCR"\AS.seek 0.01ml F*K' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F**% h % ."MFBPCR"\AS.seek 0.58ml F*H' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*c n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*!  +21 h  ."MFBPCR"\AS.seek 0ml F*B'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *\ 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*r  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *=' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*p n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +202 h 'R ."MFBPCR"\SPE.coast F*S  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*`'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*P n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *e' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*h  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*A  +64 h  F*3% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*T n +65 h n F*/% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *u' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Y I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*(  +64 h  F*)% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *;'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*V n +65 h n F*^% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*<  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *?': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*' n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *%'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*X n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*9  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*_ % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*#% h % ."MFBPCR"\AS.seek 0.96ml F*a'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*x% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*0 I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *+'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *w'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *~'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*7% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*z I +43 h I ."MFBPCR"\AS.seek 0.02ml F*,' +9880 h ' ."MFBPCR"\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*m% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*O % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*R'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*.  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*i % +108 h % ."MFBPCR"\AS.seek 0ml F*P'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990431 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '| h  P% %` h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *e  h  .t\SPE.configure SPEconfig F*\ 2 *[% h %V .t\AS.configure ASconfig S)h% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & S)#.0X 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:58:34CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #10) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'x& h & .tHand.open M&1 +150 h 1 M&0& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &$(- +166 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &w'η +91 h 'η .tHand.close M&j/ +150 h / M&U& h &- M&7% h %η .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +264 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C',& h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'W& h &W C'J% h %4 .t\CSV.dial CSR C'  +150 h  C'{% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 't% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'o +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'g'4 +2009 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'\\  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& 'l  h +75 .t\CTV.dial :intake C'>  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'y'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'2  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C'-'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'C +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'k'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'*'4 +507 h '4 C'R& h &W C'q% h %4 .t\CSV.dial :flush C'@  +38 h  .t\CS.seek 3ml C'K'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +38 h @ C'H% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&B& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +7 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&p' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'S( +416 h ( M&& h &ӷ M&i% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&M1 +150 h 1 M&b% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 16:58:40CEST26-Sep-18\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:58:34CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*P% h %&K #tLoading sh1 puck from tube 3 into Collection Clamp #"readyMFB"Readying the MFB C' & #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(|% h %X A(`. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*e% h %&K ."readyMFB"\ATV.dial :waste2 F*[ % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *h' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&n& h &- M&A% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &3( +166 h ( M&& h & A(T& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &/' +90 h ' A( & h & .t\SE.seek 15.71 Pucks below Top Plate A(Y'L +106 h 'L A(u& h & M&f& h & .t\Elbow.seek 3 M&( +7 h ( M&G& h & A((& h & .t\Forearm.seek 3 + 300 counts M&)'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down M&5& A(^'X h & M&;% h %B .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M&X(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +126 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +91 h ' .t\CC.seek :closed C'1(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'_& h &W ."airStroke"\CSV.dial :waste S)% ."airStroke"\CS.seek 0ml h % C'x'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'$9 h 9Td +100 C'~9 h 9kd +100 C' 9 h 9d +100 C',9 h 9d +100 C'9 h 9d +100 C'U9 h 9d +100 C'9 h 9d +100 C'W9 h 9d +100 C'J9 h 9 d +100 C'9 h 9 d +100 C'9 h 96d +100 C's9 h 9Ld +100 C'79 h 9bd +100 C't9 h 9xd +100 C'D9 h 9d +100 C'o9 h 9d +100 C'{9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'\\9 h 9d +100 C'l9 h 9d +100 C'9 h 9(d +100 C'>9 h 9>d +100 C' 9 h 9Td +100 C'&9 h 9id +100 C'g9 h 9d +100 C'I9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'y9 h 9d +100 C'9 h 9d +100 C'29 h 9  d +100 C'O9 h 9 d +100 C'-9 h 9 *d +100 C'9 h 9 ?d +100 C'Q9 h 9 Td +100 C'C9 h 9 j d +100 C'9 h 9 $d +100 C'k9 h 9 (d +100 C'R9 h 9 ,d +100 C'q9 h 9 0d +100 C'*9 h 9 4d +100 C'.9 h 9 8d +100 C'H9 h 9 & h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &&' +93 h '  .t\Elbow.hold M&g, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &I1 +150 h 1 M&% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&y'8 +20 h '8 M&& h & A(2& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &O' +23 h ' A(-% h % ."downElevator"\SE.seek :down A('X +100 (Q% h % M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +22 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'k @ + A(6 +18 h @ .t\CS.seek 10ml C'R' +6 h ' .t\CSV.dial between air and CTV C'q ` +19 h ` .t\AIV.dial :bypass C'* { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'.& h & .tHand.open M&H1 +2 h 1 M&K& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&}(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&@/ +150 h / M&\ & h &- M&=% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +264 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &i' +91 h ' .t\CC.seek :closed C'4(W +416 h (W C'm& h &W .tHand.open M&F1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +151 h P%M% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%P  +38 h  .t\PS.seek 0ml P%b' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%|  C'`  h +75 .t\PSV.dial :mixloop P%e +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%[' +2610 h ' .t\PSV.dial :air P%A  +37 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P%9 +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%f +37 h .t\PS.seek 0ml P%u' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&:& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +7 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M&5' +90 h ' .tHand.close M&;/ +150 h / .t\CC.seek :open C'(( +416 h ( M&3& h &ӷ M&/% h % .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &%(- +265 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&^1 +150 h 1 M&G% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'S& h & M&% h %\  A(% h %X A(_. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ("& h & M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &+( +166 h ( M&]& h & A(v& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(?& h & .t\SE.seek 14.71 Pucks below Top Plate A(j'] +116 h '] A(& h & M&w& h & .t\Elbow.seek 3 M&$( +7 h ( M&~& h & A(,& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &W/ +150 h / ."downElevator"\SE.seek :down M&x& A(J'X h & M&'% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&h(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'z(W +416 h (W .tHand.open M&U1 +150 h 1 M&#% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'1  +19 h  .t\CS.seek 5ml C'{'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%\\  +38 h  .t\PS.seek 2.5ml P%>' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%t  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%I  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'-  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'l ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'Q' +2647 h ' .t\CSV.dial CTV C'C  +38 h  .t\CS.seek 0ml C'k'4 +2647 h '4 .t\CBV.dial :waste1 C'q @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'*  +150 h C'.& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&&% h %\  A(H% h %X A(K. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (y& h &  M&& h &ӷ M&O% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &!( +142 h ( M&c& h & A(0& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 4.71 Pucks below Top Plate A(g' +215 h ' M&@& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &=' +25 h '  .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(B- h -+ M&$&.,(\ #b .t\SE.seek 5.04 Pucks below Top Plate A(N' +27 h ' M&% h %  A(}& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&E'8 +21 h '8 M&o& h & A(m& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &F' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(' +36 h ' M&p& h & M&M% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +90 h '\  .t\Elbow.seek CC M&P(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&b' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'`( +416 h ( A(e& h &  A(& h &  M& & h &ӷ M&A% h % .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &9( +142 h ( M&n& h & A(8& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h '  .t\Elbow.hold M&f, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&:% h %  A(6& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&V'8 +21 h '8 M&;& h & A(r& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Z' +22 h ' A(4% h % ."downElevator"\SE.seek :down A(3'X +100 (/% h % M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +89 h '\  +13 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%Y  + A(6 +112 h  P%T% h % .t\PS.seek 0.499ml P%^' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%G +38 h .t\PRV.dial :air P%<  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%S  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%_  +19 h  P%"% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %L' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C']& h & .tHand.open M&v1 +2 h 1 M&?& h & M&%% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &- .t\Forearm.seek :garage M&,'η +91 h 'η .tHand.close M&/ +150 h / M&W& h &- M&x% h %η .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' .t\CC.seek :closed C'j(W +416 h (W C'& h &W .tHand.open M&$1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'(& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%U @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%#  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%1% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%R  +38 h  .t\PS.seek 0ml P%{' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%\\  +38 h  .tdelay 2 seconds +200 P%u% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb % 'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%t  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%I' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%>  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%2  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %-' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%l +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%C  +75 h  .t\PS.seek 2.5ml P%Q' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%*' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%.  +75 h  .t\PS.seek 2.5ml P%&' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%H +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%K  +75 h P%y% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'O & +50 h & .t\AWV.select :waste C' +7 P%c% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %0'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %h' +1313 h ' .t\PSV.dial :air P%D  +37 h  .t\PS.seek 2.5ml P%@' +2610 h ' .t\PSV.dial :PML P%\ +38 h .t\PS.seek 0ml P%B' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'}& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*o @@T .t\AWV.select :air F*m  .t\AIV.dial :bypass .\PSV.dial PSR P%p +21 h  ."MFBPCR"delay 20 seconds +92 h P%M% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*! I +129 h I F*g% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *='6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%E +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%N' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*|  +22 h ' .t\PSV.dial PSR P%i +21 h  F*z% h %6 ."MFBPCR"\AS.seek 0.329ml F*e'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*A % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +37 h  .t\PS.seek 0ml P%b' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*P  +22 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *:' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%6 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%;' +217 ."MFBPCR"\ATV.dial :AIV F*r I +42 h I F*4% h % ."MFBPCR"\AS.seek 0.469ml F*[' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%9 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P%5 +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%V' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%Y' +739 h ' ."MFBPCR"delay 5 seconds +500 F*T9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*Z n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*3% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%G' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%< +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial PSR P% +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%_' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%"  +38 h  .t\PS.seek 0ml P%^' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%v +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %?'k +2679 h 'k .t\PSV.dial :waste1 P%%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %w' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*~ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *f'V +850 h ' .t\PSV.dial :air P%  +112 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%x +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %)' +5106 h ' .t\PSV.dial :air P%,  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %X' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*s  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *''Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*` I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*$' +171 h ' .t\PSV.dial :mixloop P%J +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %(' +619 h ' ."MFBPCR"\ATV.dial :waste F*U n +129 h n ."MFBPCR"\AS.seek :empty F*7'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*#  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *1' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*L  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *R'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'u' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  't  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C' '4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*>  +22 h ."MFBPCR"delay 3 seconds +300 F*I% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'2  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'l'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*-% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*C'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*q% h %& ."MFBPCR"\AS.seek 0.092ml F*Q'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'.' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'*  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'H'4 +2647 h '4 C'K& h &W #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &&(ӷ +7 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'0( +416 h ( M&D& h &ӷ M&h% h % .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&p1 +150 h 1 M&M% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'!& h & M&g% h %\  A(% h %X A(d. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (E& h & M&& h &- M&F% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &N( +166 h ( M&|& h & A(z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +90 h ' A(& h & ."WCR"\SE.seek 13.71 Pucks below Top Plate A(='n +125 h 'n A(& h & M&A& h & ."WCR"\Elbow.seek 3 M&e( +7 h ( M&8& h & A(P& h & ."WCR"\Forearm.seek 3 + 300 counts M& 'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +129 h '&0 ."MFBPCR"delay 3 seconds +21 h / ."downElevator"\SE.seek :down A(n'X M&6& h & M&r% h %B ."WCR"\Forearm.seek :retract M&4'\ +91 h '\  ."WCR"\Elbow.seek CC M&b(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&:& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +46 ."MFBPCR"\ATV.dial :waste2 F*5 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +2 h ' ."WCR"\CC.seek :closed C'/(W +416 h (W ."WCR"Hand.open M&1 +141 h 'R ."MFBPCR"\ATV.dial :air2 F*V  +9 h 1 M&T% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +13 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *3' +78 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)[+ +7 h + S)% h % C'Y& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'<  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*I n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*2  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *R' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 * % h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*-% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*q'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*C n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F** % +43 h % F*.% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *K'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*y I +21 h I F*O% h %v ."MFBPCR"\AS.seek 0.059ml F*c'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*+% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*D% h % ."MFBPCR"\AS.seek 0.769ml F*h' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*}' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*p% h % k ."MFBPCR"\AS.seek 0.779ml F*M'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*\ I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *!' +4416 h ' ."MFBPCR"\ATV.dial :waste F*g n +129 h n ."MFBPCR"\AS.seek :empty F*'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*d % +43 h % F*E% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*m% h % ."MFBPCR"\AS.seek 0.579ml F*|' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*@'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*A% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*8' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*P n +86 h n ."MFBPCR"\ASV.dial :wasteR F*B  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*N % +108 h % F*i% h %V ."MFBPCR"\AS.seek 0.59ml F*:' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*9 n +108 h n ."MFBPCR"\ASV.dial :wasteR F* % h %  ."MFBPCR"\AS.seek 0.09ml F*=' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*e I +86 h I F*n% h % ."MFBPCR"\AS.seek 0.12ml F*4' +625 h ' ."MFBPCR"\ATV.dial :air3 F*Q % +21 h % F*0% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*V% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h % ."MFBPCR"\AS.seek 0.255ml F*;'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*[' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *_'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*G @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*v'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*%  +43 h  #"MFBPCR"\Swish #1 of 2 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1032 h '\  F*]% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*W'\  +1031 h '\  F*,% h %\  ."MFBPCR"\AS.seek 0.5ml F*)'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*s% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *w' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*x % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +3472 h 'V ."MFBPCR"\SPE.coast F*`  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0715h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*$  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *7'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*1  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*\\  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*k' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*t'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +107 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*> n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*C  +65 h ."MFBPCR"\ARV.dial :air1 F*q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*.'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226087 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'O  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'K @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'+ @ +75 h @ +50 S)c% h % ."WCR"\CBV.dial between CSV and waste2b C'j  +95 h  C'D% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'h  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '&'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'p  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '}' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'\  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'M  +37 h  C'g% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'd' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'! @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'F  +94 h  C'm% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'z' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '|  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'A  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '@' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'P  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'B  +38 h  C'8% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'N @ +75 h @ +50 S)i% h % ."WCR"\CBV.dial between CSV and waste2b C'r  +94 h  C'9% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C':' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'5  'e  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'n'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'=  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Q' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'0  +150 h C'4& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&T% h %\  A(V% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (Y( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (<& h &  M&& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&;& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A([& h &  ."WCR"\SE.seek 5.71 Pucks below Top Plate A("' +205 h ' M&S& h & A(_& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &' +26 h '  ."WCR"\Elbow.hold M&^, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(v- h -+ M&$&.,(\ #b ."WCR"\SE.seek 6.04 Pucks below Top Plate A(%' +27 h ' M&?% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&]'8 +20 h '8 M&~& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +23 h ' ."WCR"\SE.seek 7.04 Pucks below Top Plate A(,' +36 h ' M&W& h & M&s% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +89 h '\  ."WCR"\Elbow.seek CC M&x(ӷ +143 h (ӷ M&w& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&J/ +150 h / ."WCR"\CC.seek :open C''( +416 h ( A(f& h &  A(& h &  M&3& h &ӷ M&U% h % ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &$( +142 h ( M&L& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h '  ."WCR"\Elbow.hold M&1, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&7% h %  A(\\& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&{'8 +20 h '8 M&& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &u' +22 h ' A(k% h % ."downElevator"\SE.seek :down A(I'X +100 (2% h % M&t% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +90 h '\  + 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 A(6 h 1 M&-& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&R(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&q'η +91 h 'η .tHand.close M&*/ +150 h / M&& h &- M&l% h %η .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +265 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'c(W +415 h (W C'j& h &W .tHand.open M&D1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'h% h % .t\CSV.dial CSR C'  +38 h  C'p% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'&'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '\ % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'M'[ +463 h '[ .t\CSV.dial :waste C'g +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'd& h &W C'!% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'm @ +38 h @ C'% h %p .t\CS.seek 7ml C'E'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '|  +37 h  +113  .t\CBV.dial :waste1 C'z @ +75 h @ .t\CS.seek 0ml C'A'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'P% h %4 .t\CSV.dial :flush C'B  +76 h  .t\CS.seek 5ml C'8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C' % h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  +37 h  .t\CBV.dial :waste2b C'r  +76 h  .t\CS.seek 0ml C'9'4 +765 h '4 #t\Flushing upper manifold C' & h &W C'6% h %4 .t\CSV.dial :flush C'e  +76 h  .t\CS.seek 5ml C'5'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C': @ +38 h @ C'=% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'0  '4 +37 h +76 .t\CTV.dial :air C'T  +150 h .t\CS.seek 0ml C'V'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'< +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C';% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'['0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +38 h @ C'S% h %0 .t\CS.seek 5ml C'_'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C'? +75 h .t\CS.seek 0ml C''4 +506 h '4 #tDisinfecting external Intake with mfbkill C'~& h &W C'% h %4 .t\CSV.dial CSR C'W  +151 h  C's% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'" .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'w' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'`  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ']'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'%  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'^'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'J' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  '/  +37 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'3  +37 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'L'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'7  +38 h  C'\\% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast C'#d @b .t\CS.seek 10ml C'' +710 h ' ."connect"\CSV.dial CBV C'k .t\CBV.dial CSV C'#  +38 h +75 .t\CTV.dial :air C'$  +151 h .t\CS.seek 0ml C'2'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'-& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'{'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C', @ +38 h @ C'% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u +113 h .t\CTV.dial :air .\CS.seek 0ml C'I'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C'1 +38 h .t\CS.seek 0ml C'*'4 +506 h '4 C'& h &W C'l% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'H% h %0 .t\CS.seek 5ml C'R'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'y'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C'D'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C'h  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +37 h @ C'+% h %p .t\CS.seek 7ml C'Q'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  '\  +38 h  +113  .t\CBV.dial :waste1 C'g @ +38 h @ .t\CS.seek 0ml C'M'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'd& h &W C'!% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'm @ +38 h @ C'% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'|  +76 h  .t\CS.seek 0ml C'z'4 +766 h '4 #tDisinfecting external Intake with kill C'E& h &W C'% h %4 .t\CSV.dial CSR C'P  +37 h  C'B% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'A'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'8  +37 h +76 .t\CTV.dial :intake C'i  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'r' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'N  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'6  +112 h  C'e% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'9'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C':' +1373 h ' .t\CSV.dial CBV C'= +112 h .t\CS.seek 0ml C'5'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&0& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +6 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'K% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'q'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'R'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C'j'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&'& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.seek CC M&D' +91 h ' .tHand.close M&p/ +150 h / .t\CC.seek :open C'+( +416 h ( M&& h &ӷ M&&% h % .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +265 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +90 h 'η .tHand.open M&1 +149 h 1 M&M% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  #t\Completed [coreNEG] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST08-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:00:22CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST08-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0715h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*P% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*8'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*i% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*N  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*r  +65 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*n  +32 h  ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0717h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*%  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*3  +22 h ."MFBPCR"delay 2 seconds +200 F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*L  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*]% h %Fj ."MFBPCR"\AS.seek 0.524ml F*5' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*x  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*#  +108 h  ."MFBPCR"delay 7 seconds +700 F*\\% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*t  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0717h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*C  +21 h ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *l'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*R% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +65 h  F*p% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*d  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *6'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0720h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ARV.dial :carrier F*e  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +22 h ."MFBPCR"delay 2 seconds +200 F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *c'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*b I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*4' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*_ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Z  +107 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*~'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*[  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*%'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0720h.pcr F*W% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*`  +22 h ."MFBPCR"\ATV.dial :air3 F*) % +87 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*5% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +65 h  F*#% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *,' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*{% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +21 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +21 h F*Y% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*I  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0722h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*p  +42 h  ."MFBPCR"\ARV.dial :carrier F*'  +10 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*d  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Q'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*\ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*M' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*o' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*|  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*P'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*A'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*B  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*i' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*N  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*r% h % \\ ."MFBPCR"\AS.seek 0.326ml F*@'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CEST1538951393.72 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0722h.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*:  +22 h ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*T% h %Z ."MFBPCR"\AS.seek 0.81ml F*n'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*_  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +64 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Z'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*"  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*%'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*)% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0800h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*\\% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*2  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*{% h %Fj ."MFBPCR"\AS.seek 0.524ml F*,' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*G % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*C  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +22 h n ."MFBPCR"delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.6ml F*>'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*H  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0800h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*h @@T ."MFBPCR"\ASV.dial :SPEdelivery F*J  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +151 h F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *d' +423 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*D% h % ."MFBPCR"\AS.seek 0.62ml F*\ '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*! n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*.'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*M % +43 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +424 h ' ."MFBPCR"\ATV.dial :air3 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*E' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h % H ."MFBPCR"\AS.seek 0.735ml F*B' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*A% h % Ű ."MFBPCR"\AS.seek 0.72ml F*8' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*N n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*r% h % zG ."MFBPCR"\AS.seek 0.365ml F*i' +7161 h ' ."MFBPCR"\ATV.dial :waste F*e n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*0% h % ."MFBPCR"\AS.seek 0.579ml F*9' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +21 h % ."MFBPCR"delay 3 seconds +300 F*T% h %  ."MFBPCR"\AS.seek 0.604ml F*<'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*n' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*b n +86 h n ."MFBPCR"\ASV.dial :wasteR F*;  +22 h  ."MFBPCR"\AS.seek 0ml F*c'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *S'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*4 % +108 h % F*V% h %V ."MFBPCR"\AS.seek 0.01ml F*Z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*~% h % q ."MFBPCR"\AS.seek 0.605ml F*"'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*[' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *W'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *`' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*) n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *%'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +202 h 'R ."MFBPCR"\SPE.coast F*U  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*/'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*3 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +42 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *L' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +42 h  F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*\\  +64 h  F*7% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*k n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *$' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*t  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *{': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*-  +64 h  F*G% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *u'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*, n +65 h n F*C% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Y' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F** n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *l'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*1 n +150 h n ."MFBPCR"\AS.seek 0ml F*H'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*K  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*> % +43 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*y% h % ."MFBPCR"\AS.seek 0.96ml F*''0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %0 ."MFBPCR"\AS.seek 0.985ml F*p' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*&% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *D'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *!'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Q'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *.' +9988 h ' ."MFBPCR"\ATV.dial AIV F*o I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F*} n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*P  +21 h ."MFBPCR"\ATV.dial :air3 F*E % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*N% h %Z ."MFBPCR"\AS.seek 0.81ml F*r'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*@% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +64 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F*n % +86 h % F*c% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*S'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*~% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*U n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *%'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*f % +108 h % ."MFBPCR"\AS.seek 0ml F*3'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +3562845 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )] h  C' ' h  P% %/ h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *L  h  .t\SPE.configure SPEconfig F*\ 2 *5% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'#& S)#.0X'\ +7 h '\  .t\Elbow.seek :hammer M&R(M + h '4 +91 (M .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &H' +91 h ' M&% h % .t\Forearm.seek :retract M&y'\ +91 h '\  M&q% h %\  P%w& C'J& h &2 ."openPC"\PC.reconfigure PCconfig h &3 .t\CC.reconfigure CCconfig ."openPC"\PC.home.seek :open .t\CC.home.seek :open P%$x2 ^22b %'( C'$x2 ^22b '^( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 14:00:15 began in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:01:22CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #11) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'&& h & .tHand.open M&p1 +150 h 1 M&& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&D& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &!'η +91 h 'η .tHand.close M&\ / +150 h / M&& h &- M&M% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +264 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'F& h &W .tHand.open M&1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'z% h %4 .t\CSV.dial CSR C'B  +150 h  C'N% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'A'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '8% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'e'[ +463 h '[ .t\CSV.dial :waste C'r +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '6'4 +2009 h '4 #t\Purging external Intake C'@& h &W C'i% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C': '  h +75 .t\CTV.dial :intake C'O  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'9  +75 h #t\Flushing upper manifold C'& h &W C' % h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C';'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +37 h @ C'c% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'4 +113 h .t\CTV.dial :air .\CS.seek 0ml C'V'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'v +37 h .t\CS.seek 0ml C''4 +507 h '4 C'S& h &W C's% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.seek 3ml C'Z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'"'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'['4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'U( +416 h ( M&& h &ӷ M&f% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +264 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &('η +91 h 'η .tHand.open M&j1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:01:27CEST26-Sep-18\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:01:22CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*+% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'h& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&]% h %\  A(% h %X A(/. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*L% h %&K ."readyMFB"\ATV.dial :waste2 F*5 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (#& h & M&x& h &- M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &7( +166 h ( M& & h & A(k& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h & .t\SE.seek 12.71 Pucks below Top Plate A( ' +136 h ' A($& h & M&2& h & .t\Elbow.seek 3 M&t( +7 h ( M&& h & A(G& h & .t\Forearm.seek 3 + 300 counts M&-'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &,/ +150 h / ."downElevator"\SE.seek :down M&& A(C'X h & M&u% h %B .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.seek CC M&1(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C'w(W +416 h (W .tHand.open M&J1 +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)>+ +7 h + S)H% h % C'&& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +38 h  .t\CS.seek 1.275ml C'' +919 h ' #tHeating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'   '9 h 9d +100 C'D9 h 9d +100 C'd9 h 91d +100 C'9 h 9J d +100 C'p9 h 9bd +100 C'9 h 9zd +100 C'M9 h 9d +100 C'y9 h 9d +100 C'\ 9 h 9 d +100 C'o9 h 9$d +100 C'.9 h 9(d +100 C'9 h 9 ,d +100 C'Q9 h 9$0d +100 C'^9 h 9<4d +100 C'!9 h 9S8d +100 C'P9 h 9kd +100 C' 9 h 9Td +100 C'O9 h 9kd +100 C'<9 h 9d +100 C'T9 h 9d +100 C'c9 h 9d +100 C';9 h 9d +100 C'49 h 9d +100 C'b9 h 9d +100 C'V9 h 9 d +100 C'69 h 9 d +100 C'9 h 96d +100 C'_9 h 9Ld +100 C'S9 h 9bd +100 C's9 h 9xd +100 C'n9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'~9 h 9d +100 C'Z9 h 9d +100 C'"9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'W9 h 9id +100 C'v9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'`9 h 9d +100 C'E9 h 9d +100 C'9 h 9d +100 C'f9 h 9  d +100 C'm9 h 9 d +100 C'|9 h 9 *d +100 C')9 h 9 ?d +100 C'a9 h 9 Td +100 C'j9 h 9 j d +100 C'9 h 9 $d +100 C'?9 h 9 (d +100 C'%9 h 9 ,d +100 C'+9 h 9 0d +100 C']9 h 9 4d +100 C'9 h 9 8d +100 C'/9 h 9  @ +113 h @ .t\CSV.dial :air C'* @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'D  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'd @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'p @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'M +113 h .t\CBV.dial CSV C'y  +18 h C'\ % h %4 .t\CS.seek 5ml C'o'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'.  +37 h C'% h %p .t\CS.seek 7ml C'Q'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'^ @ +38 h @ .t\CS.seek 10ml C'!' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'P ` +132 h ` #tLysing Complete C' & h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&z% h %\  A(B% h %X A(F. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (}& h &  M&R& h &ӷ M&8% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &A( +143 h ( M&r& h & A(e& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &@' +89 h ' A(i& h &  .t\SE.seek 6.71 Pucks below Top Plate A(=' +196 h ' M&N& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &:' +25 h '  .t\Elbow.hold M&9, +7 h , .t\Elbow.reconfigure TightElbow A(0- h -+ M&$&.,(\ #b .t\SE.seek 7.04 Pucks below Top Plate A([' +27 h ' M& % h %  A(O& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&<'8 +21 h '8 M&T& h & A(c& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &;' +22 h ' .t\SE.seek 8.04 Pucks below Top Plate A(4'w +36 h 'w M&b& h & M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &  A(& h &  M&~& h &ӷ M&Z% h % .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &W' +93 h '  .t\Elbow.hold M&v, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %  A(`& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&E'8 +21 h '8 M&& h & A(f& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &m' +22 h ' A(|% h %w ."downElevator"\SE.seek :down A()'X +100 (a% h % M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +81 h 'X ."downElevator"\SE.coast A(5 +8 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'? @ +19 h @ .t\CS.seek 10ml C'%' +7 h ' .t\CSV.dial between air and CTV C'+ ` +18 h ` .t\AIV.dial :bypass C'] { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&/1 +2 h 1 M&L& h & M&5% h %\  .t\Elbow.seek FlushPuck::Garage M&3(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M&h/ +150 h / M& & h &- M&k% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +265 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'X(W +416 h (W C'$& h &W .tHand.open M&,1 +150 h 1 M&#% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%G +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%7' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%u' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%-  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%Y  h .t\PS.seek 0ml P%C' +2610 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 2.5ml P%U' +2609 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.seek 0ml P%&' +2610 h ' .t\PSV.dial :air P%{  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%>' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +7 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&p/ +150 h / .t\CC.seek :open C'( +416 h ( M&M& h &ӷ M&y% h % .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &o'η +91 h 'η .tHand.open M&^1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'P& h & M& % h %\  A(z% h %X A(B. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (F( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (}& h & M&R& h &- M&8% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &r( +166 h ( M&e& h & A(i& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &1' +90 h ' A(N& h & .t\SE.seek 11.71 Pucks below Top Plate A( 'Ɛ +145 h 'Ɛ A(& h & M&0& h & .t\Elbow.seek 3 M& ( +7 h ( M&O& h & A(T& h & .t\Forearm.seek 3 + 300 counts M&c'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &b/ +150 h / ."downElevator"\SE.seek :down A(V'X M&@& h & M&A% h %B .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.seek CC M&:(ӷ +55 h 'X ."downElevator"\SE.coast A(5 +87 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'[(W +416 h (W .tHand.open M&;1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'6  +151 h  .t\CSV.dial CTV C'w  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'~'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%Z  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%s  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'` @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'f @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'|  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'" ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'a' +2647 h ' .t\CSV.dial CTV C'j  +38 h  .t\CS.seek 0ml C'?'4 +2647 h '4 .t\CBV.dial :waste1 C'+ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C']  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&/% h %\  A(L% h %X A(5. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (W( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (E& h &  M&4& h &ӷ M&m% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &x( +143 h ( M&\\& h & A()& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(!& h &  .t\SE.seek 7.71 Pucks below Top Plate A(v' +185 h ' M&h& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &k' +25 h '  .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A((- h -+ .t\SE.seek 8.04 Pucks below Top Plate A(g'w +27 h 'w M&2% h %  A(3& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A(#'f +36 h 'f M&G& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&-/ +150 h / .t\CC.seek :open C' ( +416 h ( A(t& h &  A(Y& h &  M&& h &ӷ M&J% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &H( +143 h ( M&U& h & A({& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &&' +92 h '  .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&*% h %  A(D& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&d'8 +21 h '8 M&p& h & A(K& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &l' +22 h ' A(X% h %f ."downElevator"\SE.seek :down A(M'X +100 (y% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +72 h 'X ."downElevator"\SE.coast A(5 +17 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +113 h  P%q% h % .t\PS.seek 0.499ml P%^' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%\  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%P  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%z  +18 h  .t\PRV.dial :air P%B  +19 h  P%}% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %R' +251 h ' .t\PRV.dial :waste2 P%8 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %o' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&i1 +2 h 1 M&N& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.seek :garage M&T'η +91 h 'η .tHand.close M&Q/ +150 h / M&b& h &- M&@% h %η .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'c(W +416 h (W C' & h &W .tHand.open M& 1 +150 h 1 M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%; @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%9  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%6  +76 h  .tdelay 2 seconds +200 P%w% h % .t\PS.seek 1.499ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%F' +1094 h ' .t\PSV.dial :air P%%  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%Z  +37 h  .tdelay 2 seconds +200 P%>% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%s  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%`'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%f  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %|' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%" +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%~' +2610 h ' .t\PSV.dial :air P%j  +75 h  .t\PS.seek 2.5ml P%a' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%+ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%]' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%/' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%L +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h P%E% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %4' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'm & +50 h & .t\AWV.select :waste C' +6 P%\\% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'! { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %:' +1312 h ' .t\PSV.dial :air P%h  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :PML P%n +37 h .t\PS.seek 0ml P%(' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '2& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'3& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T .t\AWV.select :air F*$  .t\AIV.dial :bypass .\PSV.dial PSR P%G +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*v% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *k'6 +66 .t\PSV.dial :waste1 P%7  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%g' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*-  +22 h ' .t\PSV.dial PSR P% +21 h  F*[% h %6 ."MFBPCR"\AS.seek 0.329ml F*t'ͯ +92 h P%Y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*J % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%{  +37 h  .t\PS.seek 0ml P%u' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b **' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%D +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%p' +217 ."MFBPCR"\ATV.dial :AIV F*K I +43 h I F*X% h % ."MFBPCR"\AS.seek 0.469ml F*C' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial PSR P% +113 h P%&% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%d' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%#  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*q9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*l n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*M% h % ."MFBPCR"\AS.seek 0.041ml F*W'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%P' +2609 h ' .t\PSV.dial PSR P% +113 h P%z% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%B' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%}  +37 h  .t\PS.seek 0ml P%^' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%R' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%i +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %N'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %0' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*O I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%Q  +113 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :mixloop P%@ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %.' +5106 h ' .t\PSV.dial :air P%T  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %1' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*_  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *A'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F* % h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +170 h ' .t\PSV.dial :mixloop P%V +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.seek :empty F*S'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*9  +86 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *w' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*o  +43 h F*<% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *%'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'F  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'Z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C's  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'f  +37 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'"'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*|% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*j'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*~ n +107 h n F*+% h %& ."MFBPCR"\AS.seek 0.092ml F*a'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'L'4 +2647 h '4 C'5& h &W #tGaraging FlushPuck in Collection Clamp M&E& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &/(ӷ +7 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&4' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C')( +416 h ( M&h& h &ӷ M&:% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &$'η +91 h 'η .tHand.open M&G1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'x& h & M&v% h %\  A(7% h %X A(I. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M&,% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &g( +166 h ( M&-& h & A([& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(Y& h & ."WCR"\SE.seek 10.71 Pucks below Top Plate A(k' +155 h ' A(& h & M&J& h & ."WCR"\Elbow.seek 3 M&t( +7 h ( M&{& h & A(& h & ."WCR"\Forearm.seek 3 + 300 counts M&'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +100 h '&0 ."MFBPCR"delay 3 seconds +50 h / ."downElevator"\SE.seek :down M&U& A(D'X h & M&K% h %B ."WCR"\Forearm.seek :retract M&X'\ +91 h '\  ."WCR"\Elbow.seek CC M&u(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&*& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +16 ."MFBPCR"\ATV.dial :waste2 F* % +42 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +33 h ' ."WCR"\CC.seek :closed C'y(W +415 h (W ."WCR"Hand.open M +112 h 'R ."MFBPCR"\ATV.dial :air2 F*d  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *q' +16 h 1 M&l% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)C+ +7 h + S)% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'\  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F* n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*f  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *`% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *%' ?x +3301 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*|% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*~% h % ."MFBPCR"\AS.seek 0.279ml F*+'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*j n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*] % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *5'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*E I +22 h I F*m% h %v ."MFBPCR"\AS.seek 0.059ml F*\\'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*! % +21 h % F*r% h %H ."MFBPCR"\AS.seek 0.069ml F*=' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*h% h % ."MFBPCR"\AS.seek 0.769ml F*:' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h % q ."MFBPCR"\AS.seek 0.794ml F*3' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*G% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*n I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *x' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +129 h n ."MFBPCR"\AS.seek :empty F*7'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*I % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*$% h % ."MFBPCR"\AS.seek 0.579ml F*-' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*J% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*{' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*(  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *K'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*g % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F**' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*H n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*k' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*t I +85 h I F*D% h % ."MFBPCR"\AS.seek 0.12ml F*X' +626 h ' ."MFBPCR"\ATV.dial :air3 F*a % +21 h % F*)% h % ."MFBPCR"\AS.seek 0.13ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*l% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h % ."MFBPCR"\AS.seek 0.255ml F*&'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*C' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*z n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *B'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*W I +86 h I ."MFBPCR"\AS.seek 0ml F*i'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*8'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *O'\  +1032 h '\  F*e% h %\  ."MFBPCR"\AS.seek 0.5ml F*Q'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*R% h %(A ."MFBPCR"\AS.seek 0.6ml F*b'\  +1032 h '\  F*T% h %\  ."MFBPCR"\AS.seek 0.5ml F*.'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*_% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *0' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*@ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0817h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*V  +10 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*w  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*Z  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*?' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*s'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*%'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*~' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*j  +65 h ."MFBPCR"\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*E % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226116 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'm  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'5 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'r @ +75 h @ +50 S)\\% h % ."WCR"\CBV.dial between CSV and waste2b C'c  +94 h  C'h% h % ."WCR"\CSV.dial between air and CTV C'= ` +19 h ` ."WCR"\CS.seek 7.288ml C'4' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  '2  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '/'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'G  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '3' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'n  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'v% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'I' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'x @ +75 h @ +50 S)7% h % ."WCR"\CBV.dial between CSV and waste2b C',  +94 h  C'$% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'[' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Y  '-  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'J  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'(  +38 h  C'{% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'U' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'g @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'K  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'*' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  't  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'D'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'k  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'a' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C')  +151 h C'X& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&#% h %\  A(l% h %X A(d. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (\ & h &  M&& h &ӷ M&p% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&&& h & A(z& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(C& h &  ."WCR"\SE.seek 8.71 Pucks below Top Plate A(}' +175 h ' M&P& h & A(B& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &W' +26 h '  ."WCR"\Elbow.hold M&^, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(i- h -+ ."WCR"\SE.seek 9.04 Pucks below Top Plate A('f +27 h 'f M&N% h %  A(8& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&e'8 +20 h '8 M&O& h & A(R& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Q' +22 h ' ."WCR"\SE.seek 10.04 Pucks below Top Plate A(T'U +37 h 'U M&b& h & M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +89 h '\  ."WCR"\Elbow.seek CC M&@(ӷ +142 h (ӷ M&0& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&V/ +150 h / ."WCR"\CC.seek :open C'A( +416 h ( A(& h &  A(& h &  M&q& h &ӷ M&;% h % ."WCR"\Forearm.seek :retract M&9'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +143 h ( M&o& h & A(<& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &6' +92 h '  ."WCR"\Elbow.hold M&w, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&S% h %  A(Z& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&F& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &>' +22 h ' A(?% h %U ."downElevator"\SE.seek :down A('X +100 (f% h % M&s% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +61 h 'X ."downElevator"\SE.coast A(5 +29 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&`1 +2 h 1 M&|& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&%(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.seek :garage M&+'η +90 h 'η .tHand.close M&]/ +150 h / M&~& h &- M&"% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +265 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'c& h &W .tHand.open M&h1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'2& h &W C':% h % .t\CSV.dial CSR C'4  +37 h  C'G% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'/'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'v +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '3'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'I& h &W C'x% h %4 .t\CSV.dial :flush C'7  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ','p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Y  '-  +37 h  +113  .t\CBV.dial :waste1 C'[ @ +76 h @ .t\CS.seek 0ml C'J'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'% h %4 .t\CSV.dial :flush C'(  +75 h  .t\CS.seek 5ml C'{'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'g'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'K  +76 h  .t\CS.seek 0ml C'H'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'U% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C't'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'k% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C') 'X  +38 h +75 .t\CTV.dial :air C'#  +150 h .t\CS.seek 0ml C'l'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'd'0 +507 h '0 .t\CSV.dial :waste C'\ +38 h .t\CS.seek 0ml C''4 +506 h '4 C'p& h &W C'&% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'C'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'P% h %0 .t\CS.seek 5ml C'B'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +507 h '0 .t\CSV.dial :waste C'N +75 h .t\CS.seek 0ml C'8'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'O& h &W C'R% h %4 .t\CSV.dial CSR C'b  +150 h  C'_% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C' 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'} .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'W  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'0' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'e'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +112 h  C'Q% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'^'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  'y  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'q  +37 h  C';% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'o'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'@  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'S  +38 h  C'Z% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'? '9  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'f'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'`'4 +506 h '4 #t\Flushing upper manifold C'|& h &W C'% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CBV C'> .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'w +38 h .t\CS.seek 0ml C']'4 +506 h '4 C'~& h &W C'"% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'+'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +37 h @ C'L% h %0 .t\CS.seek 5ml C'%'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'E'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C'c +75 h .t\CS.seek 0ml C'h'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'=& h &W C'2% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'4'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'r% h %p .t\CS.seek 7ml C'a'i +416 h 'i ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV C'/  +37 h  +113  .t\CBV.dial :waste1 C'v @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'I& h &W C'x% h %4 .t\CSV.dial :flush C'7  +75 h  .t\CS.seek 5ml C'3'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Y  +38 h  .t\CBV.dial :waste2b C'-  +75 h  .t\CS.seek 0ml C'['4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'% h %4 .t\CSV.dial CSR C'  +37 h  C'(% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'J'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ '  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'K' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'g  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'U  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'*' +1372 h ' .t\CSV.dial CBV C'k +113 h .t\CS.seek 0ml C't'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&X& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +6 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'd( +416 h ( M&p& h &ӷ M&&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&u& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&B1 +150 h 1 M&i% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&O1 +2 h 1 M&R& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &b(- +6 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&}/ +150 h / M&W& h &- M&% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &e' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'.& h &W .tHand.open M&y1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C';% h %4 .t\CSV.dial CSR C'  +113 h  C'o% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '@% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '<'[ +463 h '[ .t\CSV.dial :waste C'S +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +2010 h '4 #t\Purging external Intake C'& h &W C'F% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'f'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C's& h &W C'|% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'6'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'T% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'>'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'w'4 +506 h '4 C'& h &W C'~% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'5% h %0 .t\CS.seek 5ml C'L'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'+'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'%'0 +506 h '0 .t\CSV.dial :waste C'E +75 h .t\CS.seek 0ml C'c'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +7 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'r( +415 h ( M&4& h &ӷ M&n% h % .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &v(- +265 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &x'η +91 h 'η .tHand.open M&71 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST09-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:03:12CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST09-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0817h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Y  +22 h ."MFBPCR"\ATV.dial :air3 F*- % +86 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*('> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +64 h  F*U% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*P'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*B% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0819h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*Q% h %V ."MFBPCR"\AS.seek 0.5ml F*b'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*d n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +21 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*o  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*e% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*@  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*S' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*<  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*F'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +107 h  ."MFBPCR"delay 7 seconds +700 F*Z% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*f  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*T% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0819h25ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*j  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *"'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*]% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*+'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +21 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*I  +31 h  ."MFBPCR"\ATV.dial :air3 F*= % +87 h % F*7% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0821h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F** n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*p n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*u I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*)' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*B % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*M% h %< ."MFBPCR"\AS.seek 0.594ml F*O'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.6ml F*8'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*C  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0821h25ml.pcr F*b% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*o'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Z'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*]' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V @CEST1539036018.04 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0900h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*A  +11 h  F*0% h %V ."MFBPCR"\AS.seek 0.5ml F*V'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*I  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *a'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*-  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*Y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*3% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*g  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0900h25ml.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F**  +21 h ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\ 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*D'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*B  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*P  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*}  +32 h  ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*W% h % ."MFBPCR"\AS.seek 0.81ml F*Q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0902h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*<  +10 h  F*Z% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*? n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *|'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*j  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*] n +21 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*L  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*E' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*2% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*A  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*0 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0902h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*: @@T *V  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +150 h F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *I' +424 h ' ."MFBPCR"\ATV.dial :ASV F*= n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*7  +43 h  F*h% h % ."MFBPCR"\AS.seek 0.62ml F*/'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*x n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *,' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*Y% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*(' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*J% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*g n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*K% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*U % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*X% h % ."MFBPCR"\AS.seek 0.579ml F*H' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %  ."MFBPCR"\AS.seek 0.604ml F*\ '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*!% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*D' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*u n +86 h n ."MFBPCR"\ASV.dial :wasteR F*&  +22 h  ."MFBPCR"\AS.seek 0ml F*\\'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *P'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*) % +108 h % F*l% h %V ."MFBPCR"\AS.seek 0.01ml F*i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*z% h % ."MFBPCR"\AS.seek 0.58ml F*R' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h % q ."MFBPCR"\AS.seek 0.605ml F*}'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*C' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*W  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *b'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*Q  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*d n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*;  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*y'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*q n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *o' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *<': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*Z  +65 h  F*S% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *F'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*? n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*s  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*|  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *>'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*T n +65 h n F*j% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*~  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*] n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *"'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*w n +151 h n ."MFBPCR"\AS.seek 0ml F*L'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*5  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +21 h F*E% h % ."MFBPCR"\AS.seek 0.96ml F*A'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %0 ."MFBPCR"\AS.seek 0.985ml F*G' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*n% h % ."MFBPCR"\AS.seek 0.97ml F*4'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*= I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *x'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *a'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*,' +9880 h ' ."MFBPCR"\ATV.dial :waste F*3 n +129 h n ."MFBPCR"\AS.seek 0ml F*Y'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *J'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*g% h %Z ."MFBPCR"\AS.seek 0.81ml F*K'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*X  +64 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +86 h % F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*l% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*q'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990523 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )e h  C' ' h  P% %y h  F* *  h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *o  h  .t\SPE.configure SPEconfig F*\ 2 *t% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'9& S)#.0X  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close .t\Elbow.jog 1500 M&/ &~* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:04:12CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #12) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&G1 +150 h 1 M&=& h &M M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &7(- +166 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &x'η +91 h 'η .tHand.close M&// +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &-(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' +91 h ' .t\CC.seek :closed C'(W +416 h (W C',& h &W .tHand.open M&Y1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'{& h &W C'[% h %4 .t\CSV.dial CSR C'(  +150 h  C'g% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'J'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2009 h '4 #t\Purging external Intake C' & h &W C'% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'* .t\CBV.dial CSV C'  h +75 .t\CTV.dial :intake C'm  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'#'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'H  +75 h #t\Flushing upper manifold C'p& h &W C'!% h %4 .t\CSV.dial :flush C'\  +75 h  .t\CS.seek 5ml C'&'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +37 h @ C'\\% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CBV C') .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'l'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +507 h '0 .t\CSV.dial :waste C'M +37 h .t\CS.seek 0ml C'z'4 +507 h '4 C'P& h &W C'_% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C'i'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'R% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'}'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'W +75 h .t\CS.seek 0ml C'C'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C';( +416 h ( M&d& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &v(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &r'η +91 h 'η .tHand.open M&'1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:04:18CEST26-Sep-18\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:04:12CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*$% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C':& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&e% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(y. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( ( +6 F*o% h %&K ."readyMFB"\ATV.dial :waste2 F*t % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (9& h & M&@& h &- M&Z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &S( +166 h ( M&<& h & A(?& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(F& h & .t\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' A( & h & M&f& h & .t\Elbow.seek 3 M&s( +6 h ( M&& h & A(& h & .t\Forearm.seek 3 + 300 counts M&`'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &T/ +150 h / ."downElevator"\SE.seek :down M&|& A(j'X h & M&>% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&w(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'0(W +416 h (W .tHand.open M&V1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)L% h % C'n& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'5J +216 h '5J ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)S  +151 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S) +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'!9 h 9Td +100 C'm9 h 9kd +100 C'\ 9 h 9d +100 C'#9 h 9d +100 C'\\9 h 9d +100 C'&9 h 9d +100 C')9 h 9d +100 C'u9 h 9d +100 C'l9 h 9 d +100 C'U9 h 9 d +100 C'p9 h 96d +100 C'B9 h 9Ld +100 C'P9 h 9bd +100 C'_9 h 9xd +100 C'D9 h 9d +100 C'z9 h 9d +100 C'89 h 9d +100 C'R9 h 9d +100 C'O9 h 9d +100 C'i9 h 9d +100 C'}9 h 9d +100 C'W9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'b9 h 9id +100 C'M9 h 9d +100 C'Q9 h 9d +100 C' 9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9  d +100 C'9 h 9 d +100 C'-9 h 9 *d +100 C'.9 h 9 ?d +100 C'c9 h 9 Td +100 C''9 h 9 j d +100 C'd9 h 9 $d +100 C'N9 h 9 (d +100 C'9 h 9 ,d +100 C'$9 h 9 0d +100 C'e9 h 9 4d +100 C'9 h 9 8d +100 C'y9 h 9 9 h 9 d +100 C'29 h 9 d +100 C'~9 h 9 d +100 C'`9 h 9 d +100 C'9 h 9 1d +100 C's9 h 9 Dd +100 C'9 h 9 Xd +100 C'j9 h 9 kd +100 C'V9 h 9 ~d +100 C';9 h 9 d +100 C'L9 h 9 d +100 C'n9 h 9 d .t\CH.hold 5:00,85C C'   P h  +30000  C']9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'+'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 5ml C''p +1373 h 'p .t\CSV.dial CTV C'h  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'I @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'G @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'E  +19 h C'/% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C'7% h %p .t\CS.seek 7ml C'a'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'^ @ +37 h @ .t\CS.seek 10ml C'x' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C' ` +132 h ` #tLysing Complete C'{& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&[% h %\  A((% h %X A(,. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (Y( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (3& h &  M&%& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &J( +142 h ( M&K& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(& h &  .t\SE.seek 9.71 Pucks below Top Plate A(k' +166 h ' M&g& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &*' +25 h '  .t\Elbow.hold M&H, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(X- h -+ .t\SE.seek 10.04 Pucks below Top Plate A(C'U +27 h 'U M&!% h %  A(m& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&\ '8 +21 h '8 M&#& h & A(\\& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &&' +22 h ' .t\SE.seek 11.04 Pucks below Top Plate A()'D +36 h 'D M&u& h & M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +90 h '\  .t\Elbow.seek CC M&p(ӷ +142 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&D/ +150 h / .t\CC.seek :open C'z( +416 h ( A(8& h &  A(R& h &  M&O& h &ӷ M&i% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &W( +142 h ( M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &b' +93 h '  .t\Elbow.hold M&M, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Q1 +150 h 1 M& % h %  A( & h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'8 +21 h '8 M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(-% h %D ."downElevator"\SE.seek :down A(.'X +100 (c% h % M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +52 h 'X ."downElevator"\SE.coast A(5 +37 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'N @ +19 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C'$ ` +18 h ` .t\AIV.dial :bypass C'e { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&y1 +2 h 1 M&o& h & M&t% h %\  .t\Elbow.seek FlushPuck::Garage M&1(- +166 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&:/ +150 h / M&<& h &- M&?% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +265 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'q(W +416 h (W C' & h &W .tHand.open M&T1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%|% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%S' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%>' +1597 h ' .t\PSV.dial PSR P%2 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'  P%`  h +75 .t\PSV.dial :mixloop P%s +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%j' +2610 h ' .t\PSV.dial :air P%V  +38 h  .t\PS.seek 2.5ml P%;' +2609 h ' .t\PSV.dial :mixloop P%L +38 h .t\PS.seek 0ml P%n' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :mixloop P%4 +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +7 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&E% h % .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&^1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&{% h %\  A([% h %X A((. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (,( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (3& h & M&%& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &K( +166 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &w' +90 h ' A(g& h & .t\SE.seek 8.71 Pucks below Top Plate A(' +175 h ' A(X& h & M&6& h & .t\Elbow.seek 3 M&!( +6 h ( M&m& h & A(#& h & .t\Forearm.seek 3 + 300 counts M&\\'B +23 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &u/ +150 h / ."downElevator"\SE.seek :down A(l'X M& & h & M&J% h %B .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.seek CC M&*(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\ ' +91 h ' .t\CC.seek :closed C'C(W +416 h (W .tHand.open M&&1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'U  +150 h  .t\CSV.dial CTV C'0  +19 h  .t\CS.seek 5ml C'8'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'O'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%i  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%_  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%p +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%Q  +151 h  .t\PS.seek :empty P% ' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'-  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'} ` +19 h ` .t\CSV.dial :air C'W @ +37 h @ .t\CS.seek 10ml C'c' +2648 h ' .t\CSV.dial CTV C''  +37 h  .t\CS.seek 0ml C'N'4 +2647 h '4 .t\CBV.dial :waste1 C'$ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'e  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&y% h %\  A(o% h %X A(t. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (b( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&)& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &@( +143 h ( M&Z& h & A(.& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Y' +89 h ' A(x& h &  .t\SE.seek 10.71 Pucks below Top Plate A(M' +155 h ' M&:& h & A(<& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &?' +25 h '  .t\Elbow.hold M&d, +7 h , .t\Elbow.reconfigure TightElbow A(r- h -+ M&$&.,(\ #b .t\SE.seek 11.04 Pucks below Top Plate A(v'D +27 h 'D M&f% h %  A(1& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&F'8 +21 h '8 M&z& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &T' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(9'3 +36 h '3 M&& h & M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'( +416 h ( A(s& h &  A(& h &  M&~& h &ӷ M&V% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +143 h ( M&;& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &n' +92 h '  .t\Elbow.hold M&4, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &]1 +150 h 1 M& % h %  A(h& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&I'8 +21 h '8 M&G& h & A(5& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &"' +22 h ' A(q% h %3 ."downElevator"\SE.seek :down A('X +100 (E% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +43 h 'X ."downElevator"\SE.coast A(5 +46 h '\  .t\SC.coast .\PSV.dial :air A(6 P%7  +113 h  P%+% h % .t\PS.seek 0.499ml P%^' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%= +38 h .t\PRV.dial :air P%/  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'{  +38 h .t\PRV.dial between CBV and air P%[  +18 h  .t\PRV.dial :air P%(  +19 h  P%3% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %%' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&g& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&X(- +166 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.seek :garage M&#'η +91 h 'η .tHand.close M&a/ +150 h / M&u& h &- M& % h %η .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +264 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'& h &W .tHand.open M&!1 +150 h 1 M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%& @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%H  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%U  +76 h  .tdelay 2 seconds +200 P%0% h % .t\PS.seek 1.499ml P%\ ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%,' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%8' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%i  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%_  +75 h .tdelay 2 seconds +200 P%p% h %r .t\PS.seek 0.475ml P%Q' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %-' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%} +75 h .t\PTV.dial :PRV,avoiding: :puck P%W @ +75 h @ .t\PS.seek 0ml P%O' +2610 h ' .t\PSV.dial :air P%'  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%$ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%e' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%o +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%t  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %)' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%Z% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %.'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'x { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %*' +1312 h ' .t\PSV.dial :air P%:  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :PML P%r +37 h .t\PS.seek 0ml P%D' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'f& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'1& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*z @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%|% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*@ I +129 h I F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%S  +38 h  .t\PS.seek 0ml P%2' +1597 h ' .t\PSV.dial PSR P%F +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%v' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%T +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*`  +22 h ' .t\PSV.dial PSR P% +21 h  F*C% h %6 ."MFBPCR"\AS.seek 0.329ml F*s'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%~' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*V % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *;'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%>' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b * ' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%h +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%G' +217 ."MFBPCR"\ATV.dial :AIV F*5 I +43 h I F*q% h % ."MFBPCR"\AS.seek 0.469ml F*j' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%L +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%E' +2610 h ' .t\PSV.dial PSR P% +113 h P%n% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%9  +38 h  .t\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*+9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*" n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*b'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%=' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%{ +113 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%(' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%3  +37 h  .t\PS.seek 0ml P%^' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %g'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %6' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*m I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *4'V +849 h ' .t\PSV.dial :air P%a  +113 h  .t\PS.seek 2.5ml P%u' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%#  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %w' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*B  +43 h  F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *J'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*!' +170 h ' .t\PSV.dial :mixloop P%l +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.seek :empty F*P'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*H  +86 h  F*U% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *0' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*\ % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C',  +37 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'i'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'8  .t\CTV.dial CSV C'_  +38 h  +113  .t\CBV.dial :waste1 C'p @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*Q% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'}'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*W  +22 h F*-% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*''& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*O n +107 h n F*$% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'e  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'o'4 +2647 h '4 C't& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&x/ +150 h / .t\CC.seek :open C'.( +416 h ( M&:& h &ӷ M&*% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &<(- +264 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'@& h & M&M% h %\  A(S% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Y( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h & M&2& h &- M&T% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &v( +166 h ( M&`& h & A(C& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h & ."WCR"\SE.seek 7.71 Pucks below Top Plate A(?' +185 h ' A(d& h & M&V& h & ."WCR"\Elbow.seek 3 M&s( +7 h ( M&& h & A(& h & ."WCR"\Forearm.seek 3 + 300 counts M&]'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &~/ +70 h '&0 ."MFBPCR"delay 3 seconds +80 h / ."downElevator"\SE.seek :down A(;'X M&h& h & M&5% h %B ."WCR"\Forearm.seek :retract M&q'\ +91 h '\  ."WCR"\Elbow.seek CC M&>(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +35 ."MFBPCR"\ATV.dial :waste2 F* % +13 h (ӷ M&L& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +29 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +62 h ' ."WCR"\CC.seek :closed C'E(W +416 h (W ."WCR"Hand.open M&91 +82 h 'R ."MFBPCR"\ATV.dial :air2 F*I  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *+' +46 h 1 M&"% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)j+ +7 h + S) % h % C'7& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'/  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +38 h @ C'% h %0 .t\CS.seek 5ml C'('p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'm& h &W C'%% h %4 .t\CSV.dial CSR C'u  +150 h  C'B% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'{'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'Y' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :intake C'b  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'6' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +112 h  C'a% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'^'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'E  +37 h  +113  .t\CBV.dial :waste1 C'4 @ +38 h @ .t\CS.seek 0ml C'X'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'+  +37 h  C'&% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\ ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'R'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'P  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'p' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N 'H  +38 h +75 .t\CTV.dial :air C'!  +151 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C'-& h &W C'W% h %4 .t\CSV.dial :flush C'U  +38 h  .t\CS.seek 5ml C'8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +38 h @ C'% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'0 +38 h .t\CS.seek 0ml C'e'4 +506 h '4 C'O& h &W C'}% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'$'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'o% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +506 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C':'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'k& h &W C'*% h %4 .t\CSV.dial :flush C'f  +38 h  .t\CS.seek 5ml C')'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'K% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CTV C'r  .t\CTV.dial CSV C'y  +37 h  +113  .t\CBV.dial :waste1 C'M @ +38 h @ .t\CS.seek 0ml C'|'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'@% h %4 .t\CSV.dial :flush C'S  +75 h  .t\CS.seek 5ml C'1'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'2% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV C'`  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'C'4 +766 h '4 #tDisinfecting external Intake with kill C'F& h &W C'd% h %4 .t\CSV.dial CSR C'  +37 h  C'D% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'V'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'<' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '~  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'5' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'v  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'h  +113 h  C']% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'L' +1372 h ' .t\CSV.dial CBV C'? +113 h .t\CS.seek 0ml C's'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +6 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'I( +416 h ( M&& h &ӷ M&n% h % .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&>& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &j'η +91 h 'η .tHand.open M&(1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&m1 +2 h 1 M&%& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &u(- +6 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&{'η +91 h 'η .tHand.close M&3/ +150 h / M&b& h &- M&Y% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'& h &W .tHand.open M&E1 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'+& h &W C'&% h %4 .t\CSV.dial CSR C'X  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '\ '[ +463 h '[ .t\CSV.dial :waste C'P +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +2010 h '4 #t\Purging external Intake C'R& h &W C',% h %4 .t\CSV.dial :flush C'H  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'! .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'p  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'_& h &W C'-% h %4 .t\CSV.dial :flush C'W  +75 h  .t\CS.seek 5ml C'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'#% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +506 h '0 .t\CSV.dial :waste C'Q +38 h .t\CS.seek 0ml C'0'4 +506 h '4 C'7& h &W C'O% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +37 h @ C't% h %0 .t\CS.seek 5ml C'o'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'\\'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&J& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +7 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.seek CC M&:' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'K( +415 h ( M&)& h &ӷ M&r% h % .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +265 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &@'η +91 h 'η .tHand.open M&S1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST10-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:06:02CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST10-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0917h25ml.pcr F*2% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*`  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*D'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *]'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F*/ % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0919h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*u'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*4  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*+  +21 h ."MFBPCR"delay 2 seconds +200 F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*s' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*P' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*,'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +107 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*!'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*#% h % \\ ."MFBPCR"\AS.seek 0.326ml F*U'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0919h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *}'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*f  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *6'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*z'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*`'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*T% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0921h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ARV.dial :carrier F*]  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*/  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Z'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*> I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*.' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*['< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*m'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*% n +21 h n ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.65ml F*b' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0921h25ml.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *+'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*E% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*s% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*P  +65 h  F*H% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*8% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*U'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V @CEST1539122418.04 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1000h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*6% h %V ."MFBPCR"\AS.seek 0.5ml F*l'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*) n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*k  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *c'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*y I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*2 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*z' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.536ml F*`'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*1% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.6ml F*V'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*<'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1000h25ml.pcr F*h% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*L  +21 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b */'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*9% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*.% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*g' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*3  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*b% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*I  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *P'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1002h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +10 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*,'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*W I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*8% h %Fj ."MFBPCR"\AS.seek 0.524ml F*#' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*= % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*'  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.536ml F*U'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Q  +22 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*$  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1002h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*f @@T *l  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +150 h F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*k n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*S  +43 h  F*:% h % ."MFBPCR"\AS.seek 0.62ml F*y'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*@ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *2'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*| % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *T' +423 h ' ."MFBPCR"\ATV.dial :air3 F*z% h % ."MFBPCR"\AS.seek 0.53ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*`% h % ."MFBPCR"\AS.seek 0.71ml F*F' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*D' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*V% h % Ű ."MFBPCR"\AS.seek 0.72ml F*~' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*v n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*5% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*] n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*h % +43 h % F*<% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*q% h % ."MFBPCR"\AS.seek 0.579ml F*G' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %  ."MFBPCR"\AS.seek 0.604ml F*/'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*x% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*;' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*> n +86 h n ."MFBPCR"\ASV.dial :wasteR F*n  +22 h  ."MFBPCR"\AS.seek 0ml F*Z'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*. % +108 h % F*"% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*[% h % ."MFBPCR"\AS.seek 0.58ml F*%' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h % q ."MFBPCR"\AS.seek 0.605ml F*3'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Y% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*{ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*b  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *u'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*I n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +202 h 'R ."MFBPCR"\SPE.coast F*&  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*E'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*+ n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*R  +43 h  F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*i  +65 h  F*P% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *,'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*N n +65 h n F*p% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *!' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*_  +43 h  F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *8': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*-  +65 h  F*=% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*# n +65 h n F*'% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *7' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*O  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*e n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *}'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*0 n +151 h n ."MFBPCR"\AS.seek 0ml F*o'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*t  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +21 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*J'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*r% h % ."MFBPCR"\AS.seek 0.97ml F*)'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*k I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *:'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *c'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*2  +43 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*z I +43 h I ."MFBPCR"\AS.seek 0.02ml F*T' +9880 h ' ."MFBPCR"\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.seek 0ml F*`'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*d% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*F % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*v% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*<% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +64 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*n  +32 h  ."MFBPCR"\ATV.dial :air3 F*; % +86 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*m% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*4 % +108 h % ."MFBPCR"\AS.seek 0ml F*+'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990523 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' 'X h  P% %E h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *s% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)R% h % .t\SS.configure SSconfig C'H& S)#.0X 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:07:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #13) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'r& h & .tHand.open M&1 +150 h 1 M&k& h &M M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &S(- +166 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &@'η +91 h 'η .tHand.close M&y/ +150 h / M&2& h &- M&|% h %η .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &1' +91 h ' .t\CC.seek :closed C'd(W +416 h (W C'T& h &W .tHand.open M&`1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'C% h %4 .t\CSV.dial CSR C'D  +150 h  C'v% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'V'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '~% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ']'[ +463 h '[ .t\CSV.dial :waste C'5 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'h'4 +2009 h '4 #t\Purging external Intake C'<& h &W C'% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'L  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'9'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'G  +75 h #t\Flushing upper manifold C'& h &W C'x% h %4 .t\CSV.dial :flush C'/  +75 h  .t\CS.seek 5ml C'n'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +37 h @ C'Z% h %p .t\CS.seek 7ml C'>'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'. +113 h .t\CTV.dial :air .\CS.seek 0ml C'"'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +507 h '0 .t\CSV.dial :waste C'g +37 h .t\CS.seek 0ml C'['4 +507 h '4 C'& h &W C'B% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'%% h %0 .t\CS.seek 5ml C'Y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'3'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'{'0 +507 h '0 .t\CSV.dial :waste C'b +75 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'&( +416 h ( M&I& h &ӷ M&4% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&M& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &K'η +91 h 'η .tHand.open M&A1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:07:08CEST26-Sep-18\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:07:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*+% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'f& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(X% h %X A(E. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*s % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *R' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (H& h & M& & h &- M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &P( +166 h ( M&\ & h & A(N& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &p' +90 h ' A(,& h & .t\SE.seek 6.71 Pucks below Top Plate A(' +195 h ' A(!& h & M&& h & .t\Elbow.seek 3 M&_( +7 h ( M&W& h & A(=& h & .t\Forearm.seek 3 + 300 counts M&-'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &#/ +150 h / ."downElevator"\SE.seek :down A(''X M& & h & M&% h %B .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.seek CC M&0(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +90 h ' .t\CC.seek :closed C'6(W +416 h (W .tHand.open M&l1 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)o% h % C'r& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'"  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'g'4 +2609 h '4 C'(% h %4 S)% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'B @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'x9 h 9Td +100 C' 9 h 9kd +100 C'/9 h 9d +100 C'99 h 9d +100 C'Z9 h 9d +100 C'n9 h 9d +100 C'.9 h 9d +100 C'>9 h 9d +100 C'"9 h 9 d +100 C'h9 h 9 d +100 C'9 h 96d +100 C'(9 h 9Ld +100 C'9 h 9bd +100 C'B9 h 9xd +100 C';9 h 9d +100 C'[9 h 9d +100 C'9 h 9d +100 C'%9 h 9d +100 C'm9 h 9d +100 C'9 h 9d +100 C'39 h 9d +100 C'b9 h 9(d +100 C' 9 h 9>d +100 C'Y9 h 9Td +100 C'u9 h 9id +100 C'g9 h 9d +100 C'a9 h 9d +100 C'{9 h 9d +100 C'9 h 9d +100 C'F9 h 9d +100 C'd9 h 9d +100 C'49 h 9  d +100 C'M9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C' 9 h 9 Td +100 C'A9 h 9 j d +100 C'I9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'+9 h 9 0d +100 C'9 h 9 4d +100 C'X9 h 9 8d +100 C'E9 h 9 & h & M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&;/ +150 h / .t\CC.seek :open C'[( +416 h ( A(& h &  A(%& h &  M&m& h &ӷ M&% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &b( +142 h ( M& & h & A(Y& h &  .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 2 M&u' +93 h '  .t\Elbow.hold M&g, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &a1 +150 h 1 M&{% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&F'8 +21 h '8 M&d& h & A(4& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &M' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 ( % h %v M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C'+ ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'X& h & .tHand.open M&E1 +2 h 1 M&& h & M&s% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.seek :garage M&K'η +91 h 'η .tHand.close M&f/ +150 h / M&\ & h &- M&N% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +265 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &W' +90 h ' .t\CC.seek :closed C'\\(W +416 h (W C'!& h &W .tHand.open M +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%= +151 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%P' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%p  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%-  C'  h +75 .t\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%7  h .t\PS.seek 0ml P%'' +2610 h ' .t\PSV.dial :air P%l  +38 h  .t\PS.seek 2.5ml P%&' +2609 h ' .t\PSV.dial :mixloop P%o +38 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%8  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%) +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&e& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&|& h &ӷ M&% h % .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &2(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&^1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&% h %\  A(C% h %X A(D. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (T( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (1& h & M&& h &- M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &5( +166 h ( M&]& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &0' +90 h ' A(v& h & .t\SE.seek 5.71 Pucks below Top Plate A(L' +205 h ' A(q& h & M&x& h & .t\Elbow.seek 3 M& ( +6 h ( M&U& h & A(9& h & .t\Forearm.seek 3 + 300 counts M&Z'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &>/ +150 h / ."downElevator"\SE.seek :down A("'X M&<& h & M&V% h %B .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' .t\CC.seek :closed C'j(W +416 h (W .tHand.open M&n1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'h  +150 h  .t\CSV.dial CTV C'6  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'%' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'm'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%Y +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%B  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%a  +150 h  .t\PS.seek :empty P%{' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'd  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'4 @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'3 ` +18 h ` .t\CSV.dial :air C'b @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'A  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'+ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'X& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&E% h %\  A(% h %X A(s. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (u( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (F& h &  M&.& h &ӷ M&M% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +142 h ( M&i& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &`' +90 h ' A(@& h &  .t\SE.seek 13.71 Pucks below Top Plate A(g'n +125 h 'n M&f& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &N' +25 h '  .t\Elbow.hold M&I, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(K- h -+n .t\SE.seek 14.04 Pucks below Top Plate A(w' +27 h ' M&% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&,'8 +21 h '8 M&[& h & A(!& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &#' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(H' +36 h ' M&=& h & M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +90 h '\  .t\Elbow.seek CC M&p(ӷ +142 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&-/ +150 h / .t\CC.seek :open C'( +416 h ( A(_& h &  A(7& h &  M&O& h &ӷ M&l% h % .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &o( +143 h ( M&&& h & A(8& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &r' +92 h '  .t\Elbow.hold M&), +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&e% h %  A(:& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&& h & A(t& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +22 h ' A(\\% h % ."downElevator"\SE.seek :down A(|'X +100 (% h %- M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast .\PSV.dial :air A(6 P%S  +113 h  P%$% h % .t\PS.seek 0.499ml P%^' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%k +38 h .t\PRV.dial :air P%y  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%C  +18 h  .t\PRV.dial :air P%D  +19 h  P%1% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%~ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %z' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C']& h & .tHand.open M&1 +2 h 1 M&v& h & M&2% h %\  .t\Elbow.seek FlushPuck::Garage M&q(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&U'η +91 h 'η .tHand.close M&9/ +150 h / M&>& h &- M&<% h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C'L& h &W .tHand.open M& 1 +150 h 1 M&"% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%n @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%G  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%h  +76 h  .tdelay 2 seconds +200 P%6% h % .t\PS.seek 1.499ml P%/' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Y'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%B  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%a' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%d  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%4  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%3 +75 h .t\PTV.dial :PRV,avoiding: :puck P%b @ +75 h @ .t\PS.seek 0ml P%m' +2610 h ' .t\PSV.dial :air P%A  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%+ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%X  +75 h  .t\PS.seek 2.5ml P%E' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%s  +75 h P%F% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %.' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'M & +50 h & .t\AWV.select :waste C' +6 P%i% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1312 h ' .t\PSV.dial :air P%f  +38 h  .t\PS.seek 2.5ml P%\ ' +2610 h ' .t\PSV.dial :PML P%K +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'[& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*! @@T .t\AWV.select :air .\AIV.dial :bypass F*;  .t\PSV.dial PSR P%= +22 h  ."MFBPCR"delay 20 seconds +91 h P% % h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%`' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*g% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *N'6 +66 .t\PSV.dial :waste1 P%P  +38 h  .t\PS.seek 0ml P%*' +1597 h ' .t\PSV.dial PSR P%, +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%w' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%# +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%I' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*-  +22 h ' .t\PSV.dial PSR P%W +21 h  F*j% h %6 ."MFBPCR"\AS.seek 0.329ml F*_'ͯ +92 h P%7% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%O' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*l % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*p  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *e' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%: +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*t I +43 h I F*\\% h % ."MFBPCR"\AS.seek 0.469ml F*'' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%o +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%R +113 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%H  +38 h  .t\PS.seek 0ml P%S' +739 h ' ."MFBPCR"delay 5 seconds +500 F*$9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*} n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*|% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%{ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P% +113 h P%C% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%^' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%~ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %v'k +2679 h 'k .t\PSV.dial :waste1 P%2  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %x' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*c I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *)'V +849 h ' .t\PSV.dial :air P%9  +113 h  .t\PS.seek 2.5ml P%>' +2610 h ' .t\PSV.dial :mixloop P%< +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%U  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %0' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*(  +43 h  F*q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *V'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*L% h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +170 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*G  +86 h  F*h% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *6' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*z  +43 h F*/% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +37 h  C'%% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'B  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'Y'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*a% h %_ ."MFBPCR"\AS.seek 0.434ml F*d'ݏ +110 h '4 .t\CSV.dial CSR C'4  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'3'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*b  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*A'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*m n +107 h n F*+% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'X' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C's& h &W #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +7 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +416 h ( M&f& h &ӷ M&% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &\ (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &;'η +91 h 'η .tHand.open M&=1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C' & h & M&g% h %\  A(P% h %X A(Q. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (`( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (,& h & M&*& h &- M&#% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &w( +166 h ( M&-& h & A(j& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &W' +89 h ' A(7& h & ."WCR"\SE.seek 4.71 Pucks below Top Plate A(N' +215 h ' A(I& h & M&l& h & ."WCR"\Elbow.seek 3 M&_( +6 h ( M&8& h & A(p& h & ."WCR"\Forearm.seek 3 + 300 counts M&'B +23 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &O/ +40 h '&0 ."MFBPCR"delay 3 seconds +110 h / ."downElevator"\SE.seek :down M&:& A(&'X h & M&t% h %B ."WCR"\Forearm.seek :retract M&\\'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +100 ."MFBPCR"\ATV.dial :waste2 F*e % +24 h 'X ."downElevator"\SE.coast A(5 +18 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R + h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&H1 +52 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *$' +76 h 1 M&}% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)'+ +6 h + S){% h % C'S& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'y  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % C'U& h &W E ;3\\0\\0 ."WCR"\CTV.dial :intake ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)  +76 h  #"WCR"\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'(% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X'\  +1032 h '\  F*U% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*(% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *x' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*< % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1017h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*L  +64 h  ."MFBPCR"\ARV.dial :carrier F*"  +10 h  F*V% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*$ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +22 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*6  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*%% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*d  +43 h  ."MFBPCR"\ASV.dial :waste1 F*A  +65 h ."MFBPCR"\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226175 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'M  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C's @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)i% h % ."WCR"\CBV.dial between CSV and waste2b C'Z  +94 h  C'f% h % ."WCR"\CSV.dial between air and CTV C'? ` +19 h ` ."WCR"\CS.seek 7.288ml C'.' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'E'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'=  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '[' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'g% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'Q' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)P% h % ."WCR"\CBV.dial between CSV and waste2b C'#  +94 h  C';% h % ."WCR"\CSV.dial between air and CTV C'* ` +56 h ` ."WCR"\CS.seek 7.288ml C'j' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'-  '7  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'I  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '\ ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'8  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'p  +37 h  C'l% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'O' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ':' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'w @ +75 h @ +50 S)W% h % ."WCR"\CBV.dial between CSV and waste2b C't  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'R ` +57 h ` ."WCR"\CS.seek 7.288ml C'e' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'N  '_  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '&'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '\\' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C' & h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&H% h %\  A(}% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (S( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (o& h &  M&{& h &ӷ M&r% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &`( +143 h ( M&y& h & A(C& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A('& h &  ."WCR"\SE.seek 14.71 Pucks below Top Plate A(1'] +116 h '] M&& h & A(D& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &u' +26 h '  ."WCR"\Elbow.hold M&^, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- h -+] M&$&.,(\ #b ."WCR"\SE.seek 15.04 Pucks below Top Plate A(2' +27 h ' M&v% h %  A(~& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&]'8 +20 h '8 M&c& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &9' +22 h ' ."WCR"\SE.seek 16.04 Pucks below Top Plate A(U' +37 h ' M&>& h & M&(% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&<(ӷ +142 h (ӷ M&x& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&L' +91 h ' ."WCR"Hand.close M&"/ +150 h / ."WCR"\CC.seek :open C'V( +416 h ( A()& h &  A(q& h &  M&$& h &ӷ M&n% h % ."WCR"\Forearm.seek :retract M&G'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +143 h ( M&z& h & A(/& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &h' +92 h '  ."WCR"\Elbow.hold M&6, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &%1 +150 h 1 M&% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&B'8 +21 h '8 M&T& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(a'X +100 (4% h %X M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&1 +2 h 1 M&& h & M&b% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.seek :garage M&+'η +90 h 'η .tHand.close M&/ +150 h / M&m& h &- M&3% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +265 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'i(W +416 h (W C'Z& h &W .tHand.open M&f1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h % .t\CSV.dial CSR C'.  +37 h  C'=% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'E'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'K% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'g +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '['4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'Q& h &W C' % h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '#'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'*% h %p .t\CS.seek 7ml C','i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '-  +37 h  +113  .t\CBV.dial :waste1 C'j @ +76 h @ .t\CS.seek 0ml C'I'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'8% h %4 .t\CSV.dial :flush C'p  +75 h  .t\CS.seek 5ml C'l'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'O% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  +37 h  .t\CBV.dial :waste2b C't  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'R& h &W C':% h %4 .t\CSV.dial :flush C'_  +75 h  .t\CS.seek 5ml C'N'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +38 h @ C'% h %p .t\CS.seek 7ml C'&'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'H  +150 h .t\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'o +38 h .t\CS.seek 0ml C'{'4 +506 h '4 C'r& h &W C'y% h %4 .t\CSV.dial :flush C'C  +38 h  .t\CS.seek 3ml C'''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'D'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C'~ +75 h .t\CS.seek 0ml C'c'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'|% h %4 .t\CSV.dial CSR C'>  +150 h  C'(% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'`' +416 h ' ."connect"\CSV.dial CBV C'1 .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'u  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ']'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'2  +112 h  C'9% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'^'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C') @ +38 h @ .t\CS.seek 0ml C'q'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'$  +37 h  C'n% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV C'L  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'<  +76 h  .t\CS.seek 0ml C'%'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'T'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'G  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'4'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'Y +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'& h &W C'b% h %4 .t\CSV.dial :flush C'h  +38 h  .t\CS.seek 5ml C'B'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'd% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'a'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +506 h '0 .t\CSV.dial :waste C'6 +38 h .t\CS.seek 0ml C''4 +506 h '4 C'm& h &W C'3% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'+'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C's @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'F'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +506 h '0 .t\CSV.dial :waste C'Z +75 h .t\CS.seek 0ml C'f'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'?& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'.'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'5% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  'E  +37 h  +113  .t\CBV.dial :waste1 C'g @ +38 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'Q& h &W C' % h %4 .t\CSV.dial :flush C'P  +75 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'*% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'-  +75 h  .t\CS.seek 0ml C'j'4 +766 h '4 #tDisinfecting external Intake with kill C',& h &W C'% h %4 .t\CSV.dial CSR C'8  +37 h  C'p% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'I'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'\ ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O 'l  +38 h +75 .t\CTV.dial :intake C'W  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C't' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'w  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +607 h '4 .t\CSV.dial CSR C':  +113 h  C'_% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'e' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'N'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&H& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.seek CC M&}' +91 h ' .tHand.close M&o/ +150 h / .t\CC.seek :open C'( +416 h ( M&r& h &ӷ M&y% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &{(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &''η +91 h 'η .tHand.open M&D1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'v& h & .tHand.open M&1 +2 h 1 M&|& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &>(- +6 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&1/ +150 h / M&u& h &- M&`% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &]' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'$& h &W C'n% h %4 .t\CSV.dial CSR C'q  +113 h  C'z% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'L'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '<% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '/'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig C'#P d@b .t\CS.seek 0ml C''4 +2010 h '4 #t\Purging external Intake C'%& h &W C'T% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'4'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Y  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'b  +75 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'U% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C'a +38 h .t\CS.seek 0ml C'6'4 +506 h '4 C'S& h &W C'm% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C's% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'+'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'F +75 h .t\CS.seek 0ml C'Z'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&V& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'5( +415 h ( M&.& h &ӷ M&K% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&P1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST11-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:08:53CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST11-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1017h25ml.pcr F**% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*7  +22 h ."MFBPCR"\ATV.dial :air3 F*- % +86 h % F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *,'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*p'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*l'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*W% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*:% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +86 h % F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +21 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*D% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *~'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1019h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*)  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +21 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *q'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*z  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*]% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*<  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*/  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*G  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*b' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*4  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*h'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1019h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*A  +22 h ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*+'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*=% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *x'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +31 h  ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*7'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*#% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1021h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F*R% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*M  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*r n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*D % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*C'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*v% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*| n +21 h n ."MFBPCR"delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.6ml F*c'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*`  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1021h25ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *$'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*N% h %n ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Y  +32 h  ."MFBPCR"\ATV.dial :air3 F*4 % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*h'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*S% h %m ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1539208818.04 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1100h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*V  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*E I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F** n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*!' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*-  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.536ml F*7'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*, n +21 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*p  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*R % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1100h25ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*e  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*&'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*D  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*c % +86 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *{' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*u% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1102h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*/  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*T'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*b I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*U' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*k % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*d' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*A  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*h'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +22 h  ."MFBPCR"delay 7 seconds +700 F*m% h %< ."MFBPCR"\AS.seek 0.594ml F*6'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*F' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*='{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1102h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*"  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +150 h F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' ."MFBPCR"\ATV.dial :ASV F*? n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*P  +43 h  F*f% h % ."MFBPCR"\AS.seek 0.62ml F*E'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\\'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b **'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*X'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *#' +423 h ' ."MFBPCR"\ATV.dial :air3 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*7% h % ."MFBPCR"\AS.seek 0.71ml F*,' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h % H ."MFBPCR"\AS.seek 0.735ml F*p' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*I% h % Ű ."MFBPCR"\AS.seek 0.72ml F*l' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*t% h % zG ."MFBPCR"\AS.seek 0.365ml F*W' +7161 h ' ."MFBPCR"\ATV.dial :waste F*_ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*\ % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %  ."MFBPCR"\AS.seek 0.604ml F*o'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*@% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*&' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*y  +22 h  ."MFBPCR"\AS.seek 0ml F*i'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*}% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*C% h % ."MFBPCR"\AS.seek 0.58ml F*|' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*1'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*`% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*'' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*u  +21 h  ."MFBPCR"\AS.seek 0ml F*{'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *>'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *2'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +202 h 'R ."MFBPCR"\SPE.coast F*n  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*$ n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *z' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*L I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*%  +43 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b */': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *T'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Y I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *B': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*k% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*U n +65 h n F*A% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *S' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*d I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*m  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *h': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *3'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*6 n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*s  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*F% h % ."MFBPCR"\AS.seek 0.96ml F*V'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %0 ."MFBPCR"\AS.seek 0.985ml F*=' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*K% h % ."MFBPCR"\AS.seek 0.97ml F*.'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*? I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *f'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\\'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F**  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *X' +9988 h ' ."MFBPCR"\ATV.dial AIV F*! I +43 h I ."MFBPCR"\AS.seek 0.02ml F*#' +9880 h ' ."MFBPCR"\ATV.dial :waste F*[ n +129 h n ."MFBPCR"\AS.seek 0ml F*7'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*8  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *I'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*\ % h %n ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*e% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *M'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +86 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*|'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *2'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*) % +108 h % ."MFBPCR"\AS.seek 0ml F*$'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990523 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )] h  C' 'q h  P% % h  F* *L h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ F*\ 9\ C' 2\ .t\SPE.coast P% 0\ F*\ 9\ *\ 9\ *z  h  .t\SPE.configure SPEconfig F*\ 2 *N% h %V .t\AS.configure ASconfig S)%% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'G& S)#.0X 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:09:55CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #14) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'K& h & .tHand.open M&=1 +150 h 1 M&?& h &M M&.% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &P(- +166 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&E/ +150 h / M&*& h &- M& % h %η .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &-(ӷ +264 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &[' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'#& h &W .tHand.open M&71 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'O& h &W C'j% h %4 .t\CSV.dial CSR C'p  +150 h  C'w% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'I'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'l% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +463 h '[ .t\CSV.dial :waste C't +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ':'4 +2009 h '4 #t\Purging external Intake C'\ & h &W C'W% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'e 'R  h +75 .t\CTV.dial :intake C'M  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'H'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'r& h &W C'@% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C'y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'i% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'v +37 h .t\CS.seek 0ml C'C'4 +507 h '4 C'& h &W C'(% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'`'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'u +75 h .t\CS.seek 0ml C'''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +7 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'n( +416 h ( M&& h &ӷ M&)% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +264 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&J1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:10:00CEST26-Sep-18\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:09:55CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*Z% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&]% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(q% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (L( +6 F*z% h %&K ."readyMFB"\ATV.dial :waste2 F*N % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *%' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (G& h & M&<& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&/& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(T& h & .t\SE.seek 3.71 Pucks below Top Plate A(Y' +223 h ' A( & h & M&4& h & .t\Elbow.seek 3 M&( +7 h ( M&b& h & A(k& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &U/ +150 h / ."downElevator"\SE.seek :down A(A'X M&& h & M&% h %B .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.seek CC M&6(ӷ +133 h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'x(W +416 h (W .tHand.open M&"1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +90 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +7 h + S)% h % C'K& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'@9 h 9Td +100 C'e9 h 9kd +100 C'o9 h 9d +100 C'H9 h 9d +100 C'i9 h 9d +100 C'y9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9 d +100 C':9 h 9 d +100 C'r9 h 96d +100 C'D9 h 9Ld +100 C'9 h 9bd +100 C'(9 h 9xd +100 C'&9 h 9d +100 C'C9 h 9d +100 C'c9 h 9d +100 C'|9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'19 h 9d +100 C'u9 h 9(d +100 C'{9 h 9>d +100 C'`9 h 9Td +100 C'>9 h 9id +100 C'v9 h 9d +100 C'99 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C',9 h 9d +100 C'9 h 9d +100 C')9 h 9  d +100 C'9 h 9 d +100 C'-9 h 9 *d +100 C'09 h 9 ?d +100 C';9 h 9 Td +100 C'J9 h 9 j d +100 C'9 h 9 $d +100 C'~9 h 9 (d +100 C'29 h 9 ,d +100 C'Z9 h 9 0d +100 C']9 h 9 4d +100 C'q9 h 9 8d +100 C'9 h 9 ' +92 h '  .t\Elbow.hold M&v, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &91 +150 h 1 M&% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&,'8 +21 h '8 M&& h & A()& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(-% h % ."downElevator"\SE.seek :down A(0'X +93 h 'X ."downElevator"\SE.coast A(5 +7 (;% h %X M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'~ @ +18 h @ .t\CS.seek 10ml C'2' +7 h ' .t\CSV.dial between air and CTV C'Z ` +19 h ` .t\AIV.dial :bypass C'] { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&1 +2 h 1 M&z& h & M&N% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&5'η +91 h 'η .tHand.close M&/ +150 h / M&/& h &- M&% h %η .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +264 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &4' +91 h ' .t\CC.seek :closed C'k(W +416 h (W C'$& h &W .tHand.open M&U1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%b +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%m' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'Y  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%S  h .t\PS.seek 0ml P%A' +2609 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%K' +2610 h ' .t\PSV.dial :air P%B  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%. +38 h .t\PS.seek 0ml P% ' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&L& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +6 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'd( +416 h ( M& & h &ӷ M&F% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &*(- +264 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +91 h 'η .tHand.open M&^1 +150 h 1 M&?% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'8& h & M&O% h %\  A(j% h %X A(p. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (#( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ([& h & M&& h &- M&l% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &t( +166 h ( M&_& h & A(W& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &6' +89 h ' A(w& h & .t\SE.seek 2.71 Pucks below Top Plate A(R') +235 h ') A( & h & M&@& h & .t\Elbow.seek 3 M&e( +6 h ( M&H& h & A(i& h & .t\Forearm.seek 3 + 300 counts M&h'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&\ & A(}'X h & M&I% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&M(ӷ +143 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' + h 'X ."downElevator"\SE.coast A(5 +90 h ' .t\CC.seek :closed C''(W +416 h (W .tHand.open M&y1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C':  +150 h  .t\CSV.dial CTV C'x  +19 h  .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'|' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%{' +2610 h ' .t\PSV.dial :mixloop P%` +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%(  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%9  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C') @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'-  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'1 ` +19 h ` .t\CSV.dial :air C'u @ +37 h @ .t\CS.seek 10ml C';' +2647 h ' .t\CSV.dial CTV C'J  +38 h  .t\CS.seek 0ml C'~'4 +2647 h '4 .t\CBV.dial :waste1 C'Z @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C']  +151 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&% h %\  A(z% h %X A(N. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (,& h &  M&& h &ӷ M&0% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&<& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &7' +90 h ' A( & h &  .t\SE.seek 16.71 Pucks below Top Plate A(v'; +95 h '; M&& h & A(/& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +26 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(5- h -+; .t\SE.seek 17.04 Pucks below Top Plate A(g' +27 h ' M& % h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&T'8 +20 h '8 M&C& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +23 h ' .t\SE.seek 18.04 Pucks below Top Plate A(G' +36 h ' M&b& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'Y( +416 h ( A(& h &  A(S& h &  M&m& h &ӷ M&"% h % .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&n& h & A(B& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &K' +93 h '  .t\Elbow.hold M&., +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&L% h %  A(f& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&Q'8 +20 h '8 M&=& h & A(s& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &3' +22 h ' A(k% h % ."downElevator"\SE.seek :down A( 'X +84 h 'X ."downElevator"\SE.coast A(5 +16 (F% h %X M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  .t\SC.coast .\PSV.dial :air A(6 P%P  +113 h  P%+% h % .t\PS.seek 0.499ml P%^' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%? +38 h .t\PRV.dial :air P%E  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%8  +37 h .t\CBV.dial AIV C'O  +38 h .t\PRV.dial between CBV and air P%j  +19 h  .t\PRV.dial :air P%p  +19 h  P%[% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%l @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %!' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'_& h & .tHand.open M&W1 +2 h 1 M&w& h & M&*% h %\  .t\Elbow.seek FlushPuck::Garage M&\\(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&H'η +91 h 'η .tHand.close M&i/ +150 h / M&& h &- M&\ % h %η .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'h(W +416 h (W C'R& h &W .tHand.open M&e1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  C'd& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%y @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%:  +75 h  .tdelay 2 seconds +200 P%x% h % .t\PS.seek 1.499ml P%o' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%#' +1094 h ' .t\PSV.dial :air P%2  +38 h  .t\PS.seek 0ml P%c' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%| +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %`'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%(  +75 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%9' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%{  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%)  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %-' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%1 +75 h .t\PTV.dial :PRV,avoiding: :puck P%u @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%J  +76 h  .t\PS.seek 2.5ml P%;' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Z +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%]' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%z +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h P%,% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'0 & +50 h & .t\AWV.select :waste C' +7 P%<% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %M' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :PML P%5 +38 h .t\PS.seek 0ml P% ' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'C& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*$ @@T .t\AWV.select :air F*&  .t\AIV.dial :bypass .\PSV.dial PSR P%b +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%7' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +128 h I F*v% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%T +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%g' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%4 +21 h  F*'% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%S% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%m' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*" % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%B  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *L' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%f +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +217 ."MFBPCR"\ATV.dial :AIV F*s I +43 h I F*k% h % ."MFBPCR"\AS.seek 0.469ml F*A' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%F' +2610 h ' .t\PSV.dial PSR P%% +113 h P%K% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Q' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%G  +37 h  .t\PS.seek 0ml P%P' +740 h ' ."MFBPCR"delay 5 seconds +500 F*+9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*3% h % ."MFBPCR"\AS.seek 0.041ml F*>'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%?' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%E +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial PSR P%O +113 h P%j% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%[  +38 h  .t\PS.seek 0ml P%^' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%l +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%W +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %w'k +2678 h 'k .t\PSV.dial :waste1 P%*  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb % ' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *.'V +850 h ' .t\PSV.dial :air P%i  +113 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%\ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %X' +5107 h ' .t\PSV.dial :air P%H  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %6' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*D  +43 h  F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *I'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*Y I +43 h I F*R% h %Q ."MFBPCR"\AS.seek 0.01ml F*e' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %d' +618 h ' ."MFBPCR"\ATV.dial :waste F*y n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +85 h  F*:% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *x' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*!  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *2'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'#  +38 h  C'|% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  '(  +37 h  +113  .t\CBV.dial :waste1 C'r @ +38 h @ .t\CS.seek 0ml C'`'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*{  +21 h ."MFBPCR"delay 3 seconds +300 F*9% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C')  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'1'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*u  +21 h F*-% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*J'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*Z% h %& ."MFBPCR"\AS.seek 0.092ml F*;'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'q' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'z'4 +2648 h '4 C'N& h &W #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&M% h % .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +91 h 'η .tHand.open M&b1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'& h & M&v% h %\  A(% h %X A(a. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (7( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (T& h & M&& h &- M&U% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &g( +165 h ( M&& h & A('& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &4' +90 h ' A(S& h & ."WCR"\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': A(& h & M&"& h & ."WCR"\Elbow.seek 3 M&( +6 h ( M&B& h & A(& h & ."WCR"\Forearm.seek 3 + 300 counts M&'B +23 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &m/ +10 h '&0 ."MFBPCR"delay 3 seconds +140 h / ."downElevator"\SE.seek :down M&n& A(f'X h & M&s% h %B ."WCR"\Forearm.seek :retract M&k'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +69 ."MFBPCR"\ATV.dial :waste2 F*L % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +30 h (ӷ M&=& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' ."WCR"\CC.seek :closed C'F(W +416 h (W ."WCR"Hand.open M&G1 +23 h 'R ."MFBPCR"\ATV.dial :air2 F*Q  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *+' +106 h 1 M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)A+ +6 h + S)% h % C'P& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'E  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +75 h  ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +206 C'W% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X I +86 h I ."MFBPCR"\AS.seek 0ml F*W'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*l'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F**  +43 h  #"MFBPCR"\Swish #1 of 2 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *@'\  +1031 h '\  F*_% h %\  ."MFBPCR"\AS.seek 0.5ml F*i'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*H% h %\  ."MFBPCR"\AS.seek 0.5ml F*X'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*D% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b * ' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*\ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *.'V +3472 h 'V ."MFBPCR"\SPE.coast F*Y  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1117h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*R  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*I% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*+ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*!  +22 h ."MFBPCR"delay 2 seconds +200 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*x  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*|% h %Fj ."MFBPCR"\AS.seek 0.524ml F*#' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*c% h % ."MFBPCR"\AS.seek 0.53ml F*~' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*r % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*{ n +21 h n ."MFBPCR"delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*-  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*J  +64 h ."MFBPCR"\ARV.dial :air1 F*Z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226206 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'0  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'N @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C't @ +75 h @ +50 S)<% h % ."WCR"\CBV.dial between CSV and waste2b C'h  +95 h  C'% h % ."WCR"\CSV.dial between air and CTV C'} ` +18 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'M  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'b  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'C' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'5  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'v% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'a' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'U  +94 h  C'&% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C''' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'S  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '/' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'B  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'"% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'm' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'g @ +75 h @ +50 S)4% h % ."WCR"\CBV.dial between CSV and waste2b C's  +94 h  C'=% h % ."WCR"\CSV.dial between air and CTV C'% ` +57 h ` ."WCR"\CS.seek 7.288ml C'L' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ';'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'f' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'k& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&G% h %\  A( % h %X A(Q. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (P( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &ӷ M&K% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &7( +142 h ( M&E& h & A(O& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &j' +89 h ' A(A& h &  ."WCR"\SE.seek 17.71 Pucks below Top Plate A(['* +87 h '* M&8& h & A(p& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &>' +25 h '  ."WCR"\Elbow.hold M&^, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(W- h -+* M&$&.,(\ #b ."WCR"\SE.seek 18.04 Pucks below Top Plate A(*' +26 h ' M&w% h %  A(l& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&_'8 +21 h '8 M&@& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &i' +22 h ' ."WCR"\SE.seek 19.04 Pucks below Top Plate A(H' +36 h ' M&& h & M&D% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  ."WCR"\Elbow.seek CC M&\ (ӷ +143 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ ."WCR"\Forearm.seek CC M&R' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'I( +416 h ( A(.& h &  A(\\& h &  M&+& h &ӷ M&y% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &e( +142 h ( M&!& h & A(o& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &:' +93 h '  ."WCR"\Elbow.hold M&x, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &|1 +150 h 1 M&% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&c'8 +20 h '8 M&#& h & A(r& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +23 h ' A(~% h % ."downElevator"\SE.seek :down A(9'X +73 h 'X ."downElevator"\SE.coast A(5 +27 ()% h %X M&(% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&1 +2 h 1 M&-& h & M&u% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.seek :garage M&Z'η +91 h 'η .tHand.close M&]/ +150 h / M&& h &- M&1% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +264 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'<(W +416 h (W C'h& h &W .tHand.open M&1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'M& h &W C'% h % .t\CSV.dial CSR C'  +38 h  C'b% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '5% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C'v +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'C'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'U'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'S  +38 h  +113  .t\CBV.dial :waste1 C'' @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'B% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'"'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'm% h %p .t\CS.seek 7ml C'g'i +416 h 'i ."connect"\CSV.dial CTV C'4  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C's  +75 h  .t\CS.seek 0ml C'='4 +766 h '4 #t\Flushing upper manifold C'%& h &W C'n% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +37 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'k  +38 h +75 .t\CTV.dial :air C'G  +151 h .t\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +507 h '4 C'K& h &W C'E% h %4 .t\CSV.dial :flush C'O  +37 h  .t\CS.seek 3ml C'A'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'8% h %0 .t\CS.seek 5ml C'p'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'W'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'w'0 +506 h '0 .t\CSV.dial :waste C'l +76 h .t\CS.seek 0ml C'@'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'3% h %4 .t\CSV.dial CSR C'  +151 h  C'D% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'j'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'7' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'[ +113 h .t\CTV.dial :intake C'>  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C' ' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Y  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '_'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'*  +113 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'^'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  'F  +38 h  +113  .t\CBV.dial :waste1 C'. @ +37 h @ .t\CS.seek 0ml C'\\'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'+  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'R  +38 h  .t\CBV.dial :waste2b C'\  +75 h  .t\CS.seek 0ml C'|'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '#'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'~ '  +37 h +76 .t\CTV.dial :air C'e  +150 h .t\CS.seek 0ml C')'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'-& h &W C'u% h %4 .t\CSV.dial :flush C':  +37 h  .t\CS.seek 5ml C'c'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +37 h @ C'% h %p .t\CS.seek 7ml C'J'i +416 h 'i ."connect"\CSV.dial CBV C'9 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C'x +37 h .t\CS.seek 0ml C']'4 +507 h '4 C'& h &W C'1% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.seek 3ml C'Z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C'z% h %0 .t\CS.seek 5ml C'2'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C','4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +507 h '0 .t\CSV.dial :waste C'h +75 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'}& h &W C'M% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +38 h @ C't% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'5  '  +38 h  +113  .t\CBV.dial :waste1 C'v @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'C'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'S  +75 h  .t\CS.seek 0ml C'''4 +766 h '4 #tDisinfecting external Intake with kill C'T& h &W C' % h %4 .t\CSV.dial CSR C'B  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'/' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'm '"  +38 h +75 .t\CTV.dial :intake C'4  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C's' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'g  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '%'4 +606 h '4 .t\CSV.dial CSR C'n  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'='p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'L' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&k& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'Q( +416 h ( M&K& h &ӷ M&E% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &A'η +91 h 'η .tHand.open M&p1 +150 h 1 M&W% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'w& h & .tHand.open M&1 +2 h 1 M&3& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&D& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&j'η +90 h 'η .tHand.close M&[/ +150 h / M&>& h &- M&7% h %η .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +265 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &_' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'X& h &W .tHand.open M&F1 +150 h 1 M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'+& h &W C'y% h %4 .t\CSV.dial CSR C'\\  +113 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'R'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '\ % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'o'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'|& h &W C'#% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'e +75 h .t\CTV.dial :intake C'r  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C')'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'`  +75 h #t\Flushing upper manifold C'(& h &W C'-% h %4 .t\CSV.dial :flush C'u  +75 h  .t\CS.seek 5ml C':'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'H% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'x'4 +507 h '4 C'P& h &W C'% h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.seek 3ml C'{'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'N% h %0 .t\CS.seek 5ml C'z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'2'0 +507 h '0 .t\CSV.dial :waste C', +75 h .t\CS.seek 0ml C'h'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&I& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C't( +416 h ( M&& h &ӷ M&5% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &v(- +264 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST12-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:11:45CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST12-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1117h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *T'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*B% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*4% h %n ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*s  +65 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*K% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*8'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1119h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*.  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*+  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*!  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*_% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*\  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*o  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.536ml F*#'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*r n +21 h n ."MFBPCR"delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.6ml F*`'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*)  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1119h25ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*P % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*]% h %Z ."MFBPCR"\AS.seek 0.81ml F*N'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*b% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +32 h  ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*$'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*U% h %m ."MFBPCR"\AS.seek 0.82ml F*C'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1121h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*%% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*0  +22 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *<'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*K n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*p % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*W  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*A  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.65ml F*>' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*7  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*j% h % \\ ."MFBPCR"\AS.seek 0.326ml F**'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1121h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Y  +21 h ."MFBPCR"\ATV.dial :air3 F*X % +87 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *+'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*!'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*) % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +21 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*P% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*]' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V @CEST1539295218.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1200h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*a  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*}  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *f'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*$' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*S  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*C% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*T n +21 h n ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*4' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*g  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*s% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1200h25ml.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*L  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*p  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*8  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*[  +32 h  ."MFBPCR"\ATV.dial :air3 F*@ % +87 h % F*7% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*>% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F**'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1202h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*o  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*#'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*~ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *e'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*)  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*u I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*c% h %Fj ."MFBPCR"\AS.seek 0.524ml F*H' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*? % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*J  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*:'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*] n +21 h n ."MFBPCR"delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Z  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*M% h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1202h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +151 h F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *a' +424 h ' ."MFBPCR"\ATV.dial :ASV F*} n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *f'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *U' +424 h ' ."MFBPCR"\ATV.dial :air3 F*$% h % ."MFBPCR"\AS.seek 0.53ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*T' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*B% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*"' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*g n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*s% h % zG ."MFBPCR"\AS.seek 0.365ml F*4' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*n % +43 h % F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*k% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*;' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*E  +22 h  ."MFBPCR"\AS.seek 0ml F*<'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *8'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F* % h %V ."MFBPCR"\AS.seek 0.01ml F*W' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*O% h % ."MFBPCR"\AS.seek 0.58ml F*3' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*['\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*7% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*A' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*j n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*>  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*i  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *Y' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*X n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b **'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*Q n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +202 h 'R ."MFBPCR"\SPE.coast F*y  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*F'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*+ n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*_  +43 h  F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *!' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*R I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*|  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *o': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *#'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*~ n +65 h n F*r% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *e' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*` I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*(  +43 h  F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *c': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*-  +64 h  F*?% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *9'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*H n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *P' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *:': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*] n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *1'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*x n +150 h n ."MFBPCR"\AS.seek 0ml F*z'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*N  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*{ % +43 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*,% h % ."MFBPCR"\AS.seek 0.96ml F*I'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*b' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*5% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*} I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *f'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *S' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *q' +9987 h ' ."MFBPCR"\ATV.dial AIV F*$ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*U' +9880 h ' ."MFBPCR"\ATV.dial :waste F*C n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F* % h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*B  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +42 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*g% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*k  +65 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *0'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*; % +87 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*@  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*y n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b **'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*. % +108 h % ."MFBPCR"\AS.seek 0ml F*+'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990525 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )_ h  C' '\\ h  P% %F h  F* *R h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *!  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)|% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:12:46CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #15) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&b1 +150 h 1 M&}& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &S(ӷ +264 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &C' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'U& h &W .tHand.open M&1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'm& h &W C''% h %4 .t\CSV.dial CSR C'  +150 h  C'g% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '"% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C's +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +2009 h '4 #t\Purging external Intake C'/& h &W C'4% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'L '%  h +75 .t\CTV.dial :intake C'0  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'G'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'=  +75 h #t\Flushing upper manifold C'K& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'E'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +37 h @ C'<% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +507 h '0 .t\CSV.dial :waste C'w +37 h .t\CS.seek 0ml C'O'4 +507 h '4 C'8& h &W C'D% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'W'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'3% h %0 .t\CS.seek 5ml C'7'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'['4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'> +75 h .t\CS.seek 0ml C'A'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +7 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&X' +91 h ' .tHand.close M&Y/ +150 h / .t\CC.seek :open C'y( +416 h ( M&Q& h &ӷ M&.% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &v(- +264 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &+'η +91 h 'η .tHand.open M&V1 +150 h 1 M&t% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 17:12:51CEST26-Sep-18\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:12:46CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*&% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&_% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(\\% h %X A(F. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (R( +6 F*!% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *|' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&\ & h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&o& h & A(~& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &r' +90 h ' A(#& h & .t\SE.seek 0.71 Pucks below Top Plate A(`'K +253 h 'K A(e& h & M&)& h & .t\Elbow.seek 3 M&(( +7 h ( M&u& h & A(& h & .t\Forearm.seek 3 + 300 counts M&?'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &H/ +150 h / ."downElevator"\SE.seek :down M&J& A(-'X h & M&9% h %B .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.seek CC M&x(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&1 +150 h 1 M&c% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S){+ +7 h + S)z% h % C'5& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +150 h ."CSpush"\CSV.dial CTV .t\SS.seek 10ml S)j' +100 ."CSpush"\CSV.dial :air C' @ +38 h @ ."CSpush"\CS.reconfigure CSfast .\CS.seek 0ml C'#d @b 'i'4 +6 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S) +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C' 9 h 9Td +100 C'09 h 9kd +100 C'9 h 9d +100 C';9 h 9d +100 C'<9 h 9d +100 C'E9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9 d +100 C'n9 h 9 d +100 C'K9 h 96d +100 C'p9 h 9Ld +100 C'89 h 9bd +100 C'D9 h 9xd +100 C'G9 h 9d +100 C'O9 h 9d +100 C'@9 h 9d +100 C'39 h 9d +100 C'9 h 9d +100 C'W9 h 9d +100 C'[9 h 9d +100 C'>9 h 9(d +100 C'9 h 9>d +100 C'79 h 9Td +100 C'9 h 9id +100 C'w9 h 9d +100 C'i9 h 9d +100 C'j9 h 9d +100 C'Y9 h 9d +100 C'T9 h 9d +100 C' 9 h 9d +100 C'.9 h 9  d +100 C'd9 h 9 d +100 C'S9 h 9 *d +100 C'X9 h 9 ?d +100 C'h9 h 9 Td +100 C'V9 h 9 j d +100 C'Q9 h 9 $d +100 C'l9 h 9 (d +100 C'*9 h 9 ,d +100 C'&9 h 9 0d +100 C'_9 h 9 4d +100 C'\\9 h 9 8d +100 C'F9 h 9 ( +143 h ( M&& h & A(7& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +92 h '  .t\Elbow.hold M&w, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&j% h %  A(Y& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&T'8 +21 h '8 M& & h & A(.& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(S% h % ."downElevator"\SE.seek :down A(X'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (h% h %X M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'l @ +18 h @ .t\CS.seek 10ml C'*' +7 h ' .t\CSV.dial between air and CTV C'& ` +19 h ` .t\AIV.dial :bypass C'_ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\\& h & .tHand.open M&F1 +2 h 1 M&!& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&t(- +166 h (- M&\ & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&+'η +91 h 'η .tHand.close M&/ +150 h / M&o& h &- M&~% h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +264 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &)' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'6& h &W .tHand.open M&H1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%u +150 h P%J% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%r  +38 h  .t\PS.seek 0ml P%9' +1597 h ' .t\PSV.dial PSR P%M +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%`  C'?  h +76 .t\PSV.dial :mixloop P%( +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%-  h .t\PS.seek 0ml P%P' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%y' +2610 h ' .t\PSV.dial :mixloop P%z +37 h .t\PS.seek 0ml P%5' +2610 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.seek 2.5ml P%R' +2609 h ' .t\PSV.dial :mixloop P%} +38 h .t\PS.seek 0ml P%{' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&]& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&|' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&,% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &$'η +91 h 'η .tHand.open M&^1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'B& h & M&m% h %\  A('% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (U( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (C& h & M&2& h &- M&"% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &s( +166 h ( M&& h & A(4& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(g& h & .t\SE.seek -0.29 Pucks below Top Plate A(%'\\ +264 h '\\ A(k& h & M& & h & .t\Elbow.seek 3 M&0( +7 h ( M&;& h & A(<& h & .t\Forearm.seek 3 + 300 counts M&:'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A(/'X h & M&% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +30 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'A(W +416 h (W .tHand.open M&E1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'n  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'@'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'3' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%7 +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%D  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%K +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%i  +150 h  .t\PS.seek :empty P%j' +2610 h ' .t\CBV.dial :waste1 C'Y @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'. @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'S  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'[ ` +19 h ` .t\CSV.dial :air C'> @ +38 h @ .t\CS.seek 10ml C'h' +2647 h ' .t\CSV.dial CTV C'V  +38 h  .t\CS.seek 0ml C'l'4 +2647 h '4 .t\CBV.dial :waste1 C'& @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'_  +150 h C'\\& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&F% h %\  A(!% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (T& h &  M&& h &ӷ M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &d( +142 h ( M&\ & h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(w' +65 h ' M&& h & A(o& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &~' +26 h '  .t\Elbow.hold M&Q, +6 h , .t\Elbow.reconfigure TightElbow A(+- h -+ M&$&.,(\ #b .t\SE.seek 20.04 Pucks below Top Plate A(v' +27 h ' M&e% h %  A(t& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&#'8 +20 h '8 M&O& h & A(6& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +23 h ' .t\SE.seek 21.04 Pucks below Top Plate A(' +36 h ' M&u& h & M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +89 h '\  .t\Elbow.seek CC M&r(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.seek CC M&9' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'?( +416 h ( A((& h &  A(-& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +142 h ( M&y& h & A(c& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &5' +93 h '  .t\Elbow.hold M&}, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&]% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&a'8 +20 h '8 M&b& h & A(N& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &1' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +54 h 'X ."downElevator"\SE.coast A(5 +46 (,% h %X M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +90 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%Z% h % .t\PS.seek 0.499ml P%^' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%B  +37 h .t\CBV.dial AIV C'm  +38 h .t\PRV.dial between CBV and air P%'  +19 h  .t\PRV.dial :air P%  +19 h  P%C% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %2' +250 h ' .t\PRV.dial :waste2 P%" @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %$' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&41 +2 h 1 M&g& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&f(- +166 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&;'η +91 h 'η .tHand.close M& @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%V  +76 h  .t\PS.seek 2.5ml P%h' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%& +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%_' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%\\  +76 h  .t\PS.seek 2.5ml P%F' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%! +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%T% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'X & +50 h & .t\AWV.select :waste C' +7 P%\ % h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%o  +37 h  .t\PS.seek 2.5ml P%L' +2610 h ' .t\PSV.dial :PML P%+ +38 h .t\PS.seek 0ml P%e' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 't& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'O& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*6 @@T .t\AWV.select :air F*u  .t\AIV.dial :bypass .\PSV.dial PSR P%J +22 h  ."MFBPCR"delay 20 seconds +91 h P%G% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*d I +128 h I F*w% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *~'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%M' +1597 h ' .t\PSV.dial PSR P%# +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%v' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Q' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*`  +22 h ' .t\PSV.dial PSR P%) +21 h  F*A% h %6 ."MFBPCR"\AS.seek 0.329ml F*('ͯ +92 h P%-% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *y'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%c  +38 h  .t\PS.seek 0ml P%9' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*r  +22 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *]' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%b' +217 ."MFBPCR"\ATV.dial :AIV F*N I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*P' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%,' +2610 h ' .t\PSV.dial PSR P%| +113 h P%5% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%a' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*Z9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*1% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%j +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%B' +2610 h ' .t\PSV.dial PSR P%m +113 h P%'% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%C  +38 h  .t\PS.seek 0ml P%^' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%" +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%2' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %g'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %k' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *}'V +850 h ' .t\PSV.dial :air P%<  +113 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %q' +5107 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %x' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*p  +43 h  F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*? I +43 h I F*:% h %Q ."MFBPCR"\AS.seek 0.01ml F*0' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.seek :empty F*8'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*=  +85 h  F*n% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*$  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b **'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'U  +38 h  C'3% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'{' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  'D  +37 h  +113  .t\CBV.dial :waste1 C'K @ +38 h @ .t\CS.seek 0ml C'7'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*i% h %_ ."MFBPCR"\AS.seek 0.434ml F* 'ݏ +110 h '4 .t\CSV.dial CSR C'.  +38 h  C'Y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'['P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*>  +21 h F*S% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*V'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*&% h %& ."MFBPCR"\AS.seek 0.092ml F*h'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'\\' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'!'4 +2648 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&o& h &ӷ M&% h % .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &L(- +264 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &u'η +91 h 'η .tHand.open M&J1 +150 h 1 M&G% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'd& h & M&w% h %\  A(% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +357 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (#& h &,9 M&M& h &- M&H% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &v(w +166 h (w M&`& h &w A(A& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &)' +89 h ' A(-& h &,9 ."WCR"\SE.seek 20.71 Pucks below Top Plate A(~' +57 h ' A(Q& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M&((w +6 h (w M&c& h &w A(r& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&R'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +42 h '&0 ."MFBPCR"delay 3 seconds +108 h / ."downElevator"\SE.seek :down M&& A(y'X h &w M&N% h %7 ."WCR"\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."WCR"\Elbow.seek CC M&9(ӷ +101 ."MFBPCR"\ATV.dial :waste2 F*] % +42 h (ӷ M&z& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &|' + h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +90 h ' ."WCR"\CC.seek :closed C',(W +416 h (W ."WCR"Hand.open M&1 +54 h 'R ."MFBPCR"\ATV.dial :air2 F*a  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *Z' +75 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)P+ +6 h + S)j% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*S% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b * ' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*&'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*V n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*_ % +43 h % F*\\% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*T I +21 h I F*X% h %v ."MFBPCR"\AS.seek 0.059ml F*\ 'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*s% h %H ."MFBPCR"\AS.seek 0.069ml F*/' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*o% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h % q ."MFBPCR"\AS.seek 0.794ml F*O' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*J% h % k ."MFBPCR"\AS.seek 0.779ml F*G'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*+ I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *d' +4416 h ' ."MFBPCR"\ATV.dial :waste F*w n +129 h n ."MFBPCR"\AS.seek :empty F*'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*H% h % ."MFBPCR"\AS.seek 0.579ml F*`' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %  ."MFBPCR"\AS.seek 0.604ml F*L'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*c' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*r n +86 h n ."MFBPCR"\ASV.dial :wasteR F*e  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *N'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*v % +107 h % F*)% h %V ."MFBPCR"\AS.seek 0.59ml F*z' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*b n +107 h n ."MFBPCR"\ASV.dial :wasteR F*]% h %  ."MFBPCR"\AS.seek 0.09ml F*~' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*y I +86 h I F*(% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*h % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*a' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*j' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*P' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*m n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*'  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F* @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*4'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*"'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b * '\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*<'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*2% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*;% h %\  ."MFBPCR"\AS.seek 0.5ml F*q'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*p% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *k' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +3472 h 'V ."MFBPCR"\SPE.coast F*?  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1217h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*:  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*f'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*0  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*3% h %Fj ."MFBPCR"\AS.seek 0.524ml F*U' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.53ml F*l' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*K % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*.% h %< ."MFBPCR"\AS.seek 0.594ml F*Y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.6ml F**'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*S  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*V  +64 h ."MFBPCR"\ARV.dial :air1 F*&  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226174 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'X  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C's @ +75 h @ +50 S)\ % h % ."WCR"\CBV.dial between CSV and waste2b C'%  +94 h  C'o% h % ."WCR"\CSV.dial between air and CTV C'/ ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C't  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'F'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'J  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'O' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'+  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'G  +38 h  C'w% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'd @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'M  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C'# ` +56 h ` ."WCR"\CS.seek 7.288ml C'A' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'`  '-  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Q'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'L' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'r  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'e  +37 h  C'c% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'v @ +75 h @ +50 S))% h % ."WCR"\CBV.dial between CSV and waste2b C'N  +94 h  C'b% h % ."WCR"\CSV.dial between air and CTV C'] ` +56 h ` ."WCR"\CS.seek 7.288ml C'z' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'R  'y  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '('4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'~  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'h' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(% h %X A(a. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (5& M&j& h & &ӷ ."raiseStack"\SE.seek :upperLimit A(|'Z ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&'' +91 h ' ."WCR"Hand.close M&P/ +150 h / ."WCR"\CC.seek :open C'C( +23 h 'Z +393 ( A(B& h & A(& h & M&& h &ӷ M&^% h % ."WCR"\Forearm.seek :retract M&4'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&g& h & A("& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &21 +150 h 1 M&<% h % A(;& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&p& h & A(q& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +23 h ' A(k% h %Z ."downElevator"\SE.seek :down A(?'X +100 (:% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'}& h & .tHand.open M&f1 +2 A(6 h 1 M&Z& h & M&E% h %\  .t\Elbow.seek FlushPuck::Garage M&=(- +166 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&n/ +150 h / M& & h &- M&3% h %η .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +264 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C'l& h &W .tHand.open M&i1 +150 h 1 M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'7% h % .t\CSV.dial CSR C'Y  +38 h  C'S% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'>'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '*% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'V +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '&'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'_& h &W C'% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'T'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +37 h @ C'% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CTV C's  .t\CTV.dial CSV C'\  +38 h  +113  .t\CBV.dial :waste1 C'% @ +75 h @ .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'/& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C't'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'J% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'+  +38 h  .t\CBV.dial :waste2b C'G  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'O& h &W C' % h %4 .t\CSV.dial :flush C'd  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +38 h @ C'H% h %p .t\CS.seek 7ml C'#'i +415 h 'i ."connect"\CSV.dial CBV C'u .t\CBV.dial CSV C'-  +38 h +75 .t\CTV.dial :air C'`  +151 h .t\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'Q +37 h .t\CS.seek 0ml C'r'4 +507 h '4 C'e& h &W C'c% h %4 .t\CSV.dial :flush C'L  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +38 h @ C')% h %0 .t\CS.seek 5ml C'N'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'b'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'R'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'y& h &W C'z% h %4 .t\CSV.dial CSR C'~  +151 h  C'(% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'a' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'5  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'm% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'P'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'1  ',  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'^% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'g'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'2'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'<  +38 h  C';% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +710 h ' ."connect"\CSV.dial CBV C'k .t\CBV.dial CSV C'4  +38 h +75 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C':'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'}'4 +507 h '4 #t\Flushing upper manifold C'f& h &W C'Z% h %4 .t\CSV.dial :flush C'E  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C' % h %p .t\CS.seek 7ml C'0'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'$ +113 h .t\CTV.dial :air .\CS.seek 0ml C'?'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'n'4 +506 h '4 C' & h &W C'3% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'U% h %0 .t\CS.seek 5ml C'='p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'8'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'l +75 h .t\CS.seek 0ml C'i'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'.& h &W C'% h %4 .t\CSV.dial :flush C'7  +37 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +37 h @ C'K% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'*  '>  +38 h  +113  .t\CBV.dial :waste1 C'V @ +37 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'_& h &W C'% h %4 .t\CSV.dial :flush C'[  +76 h  .t\CS.seek 5ml C'&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C's  +37 h  .t\CBV.dial :waste2b C'\  +75 h  .t\CS.seek 0ml C'%'4 +766 h '4 #tDisinfecting external Intake with kill C'!& h &W C'/% h %4 .t\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'o'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C't 'J  +37 h +76 .t\CTV.dial :intake C'+  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'G' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'F  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'O'4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'd% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'M' +1372 h ' .t\CSV.dial CBV C'H +113 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&-& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +6 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.seek CC M&A' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'( +416 h ( M&e& h &ӷ M&c% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&N1 +150 h 1 M&b% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C']& h & .tHand.open M&y1 +2 h 1 M&z& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &~(- +7 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &j' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'1& h &W .tHand.open M&,1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'^% h %4 .t\CSV.dial CSR C'  +113 h  C'g% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '"'[ +462 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ';'4 +2010 h '4 #t\Purging external Intake C'2& h &W C'p% h %4 .t\CSV.dial :flush C'4  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'q  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C':'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'f% h %4 .t\CSV.dial :flush C'Z  +76 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'|% h %p .t\CS.seek 7ml C' 'i +415 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'$'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +507 h '0 .t\CSV.dial :waste C'? +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'& h &W C' % h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +38 h @ C'@% h %0 .t\CS.seek 5ml C'U'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +507 h '0 .t\CSV.dial :waste C'8 +75 h .t\CS.seek 0ml C'l'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&C& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&S/ +150 h / .t\CC.seek :open C'K( +416 h ( M&Y& h &ӷ M&*% h % .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +264 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&[1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST13-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:14:34CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST13-Oct-18" +264322 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1217h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*s  +22 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +21 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F*+% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*#  +31 h  ."MFBPCR"\ATV.dial :air3 F*Q % +87 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*e% h % ."MFBPCR"\AS.seek 0.81ml F*c'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*N% h %m ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*L  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *]'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1219h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*m% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*g  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*j% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*"  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*4  +108 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*f% h %\  ."MFBPCR"\AS.seek 0.65ml F*Z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*:  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*}  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*|% h % \\ ."MFBPCR"\AS.seek 0.326ml F*E'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1219h25ml.pcr F*$% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*0  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*n% h %Z ."MFBPCR"\AS.seek 0.81ml F*@'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*=% h %n ."MFBPCR"\AS.seek 0.82ml F*C'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +65 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*_  +32 h  ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*T% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1221h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*d  +10 h  F*O% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *-'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*W  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *{'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*e n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*D% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*v I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*c% h % ."MFBPCR"\AS.seek 0.53ml F*u' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*N % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.536ml F*L'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*b  +107 h  ."MFBPCR"delay 7 seconds +700 F*]% h %< ."MFBPCR"\AS.seek 0.594ml F*y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*z n +21 h n ."MFBPCR"delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1221h25ml.pcr F*~% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*5  +21 h ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*,% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*E'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*?  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*n' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V @CEST1539381618.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1300h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*C  +10 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*_  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*> I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.536ml F*s'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +107 h  ."MFBPCR"delay 7 seconds +700 F*&% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.65ml F*+' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*J  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*O % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1300h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*M  +22 h ."MFBPCR"\ATV.dial :air3 F*H % +86 h % F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*#'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*c% h %n ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*N  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*u% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*]' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*1% h %m ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *<'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1302h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ARV.dial :carrier F*"  +11 h  F*;% h %V ."MFBPCR"\AS.seek 0.5ml F*p'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*:  +22 h ."MFBPCR"delay 2 seconds +200 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *f'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*Z I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*0  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*E'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*?  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +21 h n ."MFBPCR"delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*U  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.65ml F*8' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1302h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*7 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*9  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*P  +151 h F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *_' +424 h ' ."MFBPCR"\ATV.dial :ASV F*. n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*[  +43 h  F*i% h % ."MFBPCR"\AS.seek 0.62ml F*>'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*\\'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *T' +424 h ' ."MFBPCR"\ATV.dial :air3 F*6% h % ."MFBPCR"\AS.seek 0.53ml F*/' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*s% h % ."MFBPCR"\AS.seek 0.71ml F*!' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F*o% h % Ű ."MFBPCR"\AS.seek 0.72ml F*J' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*F n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*G% h % zG ."MFBPCR"\AS.seek 0.365ml F*+' +7161 h ' ."MFBPCR"\ATV.dial :waste F*d n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*-% h % ."MFBPCR"\AS.seek 0.579ml F*w' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % ."MFBPCR"delay 3 seconds +300 F*`% h %  ."MFBPCR"\AS.seek 0.604ml F*Q'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*D% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*#' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*v n +86 h n ."MFBPCR"\ASV.dial :wasteR F*c  +22 h  ."MFBPCR"\AS.seek 0ml F*{'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *)'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*u % +108 h % F*A% h %V ."MFBPCR"\AS.seek 0.01ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*L% h % ."MFBPCR"\AS.seek 0.58ml F*z' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*r'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *~'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *5' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*1 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +202 h 'R ."MFBPCR"\SPE.coast F*^  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*,'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*j  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *g' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*2  +43 h  F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *"': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*;  +65 h  F*<% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *p'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*k n +65 h n F*q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*f  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *$'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*| n +65 h n F*0% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *E': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*n n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *3'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*U'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*@  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*8% h % ."MFBPCR"\AS.seek 0.96ml F*C'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %0 ."MFBPCR"\AS.seek 0.985ml F*S' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F**% h % ."MFBPCR"\AS.seek 0.97ml F*Y'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*. I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *i'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *\ ' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *\\' +9987 h ' ."MFBPCR"\ATV.dial AIV F*6 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*T' +9880 h ' ."MFBPCR"\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.seek 0ml F*s'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*/% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +42 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*+'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*H  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +65 h  F*M% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*c  +32 h  ."MFBPCR"\ATV.dial :air3 F*# % +87 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*)'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*b'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*R  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*^ n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*B % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990525 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )j h  C' ' h  P% %, h  F* *' h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *g  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)2% h % .t\SS.configure SSconfig C'4& S)#.0X 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:34CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #16) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&S1 +150 h 1 M&.& h &M M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &[(- +166 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&>/ +150 h / M&& h &- M& % h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\ (ӷ +264 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +91 h ' .t\CC.seek :closed C'/(W +416 h (W C'T& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C't& h &W C'%% h %4 .t\CSV.dial CSR C'  +150 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'o'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'J% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'd'[ +463 h '[ .t\CSV.dial :waste C'G +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2009 h '4 #t\Purging external Intake C'& h &W C'+% h %4 .t\CSV.dial :flush C'H  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '-' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M 'O  h +75 .t\CTV.dial :intake C'W  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'`'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'w  +75 h #t\Flushing upper manifold C'e& h &W C'D% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C'c'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +37 h @ C'{% h %p .t\CS.seek 7ml C'v'i +416 h 'i ."connect"\CSV.dial CBV C'u .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'N'0 +507 h '0 .t\CSV.dial :waste C'] +37 h .t\CS.seek 0ml C'L'4 +507 h '4 C')& h &W C'(% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'b'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'z% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&r& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.seek CC M&1' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C'^( +416 h ( M&& h &ӷ M&B% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &X(- +264 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:15:40CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:15:34CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*K% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'7& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&j% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(,. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ('(,9 +6 F*g% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *2' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (4& h &,9 M&& h &- M&;% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &<(w +166 h (w M&"& h &w A(k& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &q' +89 h ' A(p& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &,9 M&:& h &w .t\Elbow.seek 4 M&(w +6 h (w M&Z& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&f'7 +23 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &|/ +150 h / ."downElevator"\SE.seek :down M&}& A(0'X h &w M&$% h %7 .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'a(W +416 h (W .tHand.open M&91 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)U% h % C'*& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9 d +100 C'69 h 9$d +100 C'\\9 h 9(d +100 C'[9 h 9 ,d +100 C'h9 h 9$0d +100 C'P9 h 9<4d +100 C'9 h 9S8d +100 C'9 h 9kd +100 C'D9 h 9Td +100 C'W9 h 9kd +100 C'Q9 h 9d +100 C'`9 h 9d +100 C'{9 h 9d +100 C'c9 h 9d +100 C'u9 h 9d +100 C'v9 h 9d +100 C'A9 h 9 d +100 C' 9 h 9 d +100 C'e9 h 96d +100 C'N9 h 9Ld +100 C')9 h 9bd +100 C'(9 h 9xd +100 C'#9 h 9d +100 C'L9 h 9d +100 C'R9 h 9d +100 C'z9 h 9d +100 C'y9 h 9d +100 C'b9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'r9 h 9>d +100 C'9 h 9Td +100 C'~9 h 9id +100 C']9 h 9d +100 C'm9 h 9d +100 C'9 h 9d +100 C'59 h 9d +100 C'!9 h 9d +100 C'/9 h 9d +100 C'B9 h 9  d +100 C'19 h 9 d +100 C'\ 9 h 9 *d +100 C'V9 h 9 ?d +100 C'I9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'K9 h 9 0d +100 C'j9 h 9 4d +100 C'9 h 9 8d +100 C',9 h 9 % h %4 .t\CS.seek 5ml C'6'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'\\  +37 h C'[% h %p .t\CS.seek 7ml C'h'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'P @ +37 h @ .t\CS.seek 10ml C'' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C' ` +132 h ` #tLysing Complete C't& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&%% h %\  A(% h %X A(T. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (s( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (&& h & M&=& h &ӷ M&J% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &o( +143 h ( M&G& h & A(d& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(+& h & .t\SE.seek -0.29 Pucks below Top Plate A(H'\\ +264 h '\\ M&F& h & A(O& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &M' +26 h ' .t\Elbow.hold M&w, +6 h , .t\Elbow.reconfigure TightElbow A(-- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&D% h % A(W& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&Q'B +20 h 'B M&`& h & A({& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &c' +23 h ' .t\SE.seek 1.04 Pucks below Top Plate A(u' +36 h ' M&v& h & M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&(' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'L( +416 h ( A(R& h & A(z& h & M&y& h &ӷ M&b% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&r& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &~' +93 h ' .t\Elbow.hold M&], +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &m1 +150 h 1 M&% h % A(5& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&!'B +20 h 'B M&/& h & A(B& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &1' +22 h ' A(\ % h % ."downElevator"\SE.seek :down A(V'X +100 (I% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'K ` +19 h ` .t\AIV.dial :bypass C'j { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&,1 +2 h 1 M&g& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&l(- +166 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&7/ +150 h / M&"& h &- M&k% h %η .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'& h &W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%q  +38 h  .t\PS.seek 0ml P%3' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'f  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%9  +37 h  .t\PS.seek 2.5ml P%^' +2610 h ' .t\PSV.dial :mixloop P%U +38 h .t\PS.seek 0ml P%*' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%'' +2610 h ' .t\PSV.dial :mixloop P%Y +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&n& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&2' +91 h ' .tHand.close M&S/ +150 h / .t\CC.seek :open C' ( +416 h ( M& & h &ӷ M&8% h % .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&P1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'& h & M&t% h %\  A(%% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (T(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (&& h &,9 M&=& h &- M&J% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&d& h &w A(+& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(E& h &,9 .t\SE.seek 18.71 Pucks below Top Plate A(F' +77 h ' A(O& h &,9 M&-& h &w .t\Elbow.seek 4 M&D(w +7 h (w M&W& h &w A(`& h &,9 .t\Forearm.seek 4 + 300 counts M&{'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &v/ +150 h / ."downElevator"\SE.seek :down M&& A(A'X h &w M&o% h %7 .t\Forearm.seek :retract M&H'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&M(ӷ +142 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&c1 +150 h 1 M&w% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'a  +18 h  .t\CS.seek 5ml C'R'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'z' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'y'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%b  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%(  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%e +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%m  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'5 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'/  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'B @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'~'4 +2648 h '4 .t\CBV.dial :waste1 C' @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'!  +151 h C'K& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&j% h %\  A(% h %X A(,. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (g( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&u& h &ӷ M&1% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &@( +143 h ( M&4& h & A(V& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &s' +89 h ' A(& h & .t\SE.seek 0.71 Pucks below Top Plate A(]'K +254 h 'K M&7& h & A("& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &k' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+K .t\SE.seek 1.04 Pucks below Top Plate A(X' +26 h ' M&:% h % A(l& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&p'B +21 h 'B M&L& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &}' +22 h ' .t\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M&& h & M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +89 h '\  .t\Elbow.seek CC M&q(ӷ +143 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&3' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'f( +416 h ( A(0& h & A(& h & M& & h &ӷ M&9% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &U( +142 h ( M&^& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &*' +93 h ' .t\Elbow.hold M&Y, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &'1 +150 h 1 M&n% h % A(i& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&_'B +20 h 'B M&S& h & A(;& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &|' +23 h ' A(x% h % ."downElevator"\SE.seek :down A( 'X +100 (8% h %B M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%[  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%P' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +38 h .t\PRV.dial :air P%>  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C't  +38 h .t\PRV.dial between CBV and air P%%  +19 h  .t\PRV.dial :air P%  +18 h  P%&% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +251 h ' .t\PRV.dial :waste2 P%J @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'd& h & .tHand.open M&+1 +2 h 1 M&E& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&O(- +166 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &- .t\Forearm.seek :garage M&`'η +91 h 'η .tHand.close M&h/ +150 h / M&v& h &- M&% h %η .t\Forearm.seek :retract M&o'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &)' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C'F& h &W .tHand.open M&A1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  C' & h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%w  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%a% h % .t\PS.seek 1.499ml P%Q' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%R' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%z +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%b  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%(  +76 h .tdelay 2 seconds +200 P%e% h %r .t\PS.seek 0.475ml P%m' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%r  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%5'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%/  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%B  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%!' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%K  +75 h  .t\PS.seek 2.5ml P%j' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%,  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %u' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'1 & +50 h & .t\AWV.select :waste C' +7 P%4% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %V'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %M' +1313 h ' .t\PSV.dial :air P%#  +37 h  .t\PS.seek 2.5ml P%7' +2610 h ' .t\PSV.dial :PML P%" +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 ':& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'l& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*L @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%$% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%s' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*@ I +129 h I F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *k'6 +66 .t\PSV.dial :waste1 P%<  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%p +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%X' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%Z +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*9 % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *^'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%3' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*q  +21 h  F*'% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *n' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%i +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%S' +216 ."MFBPCR"\ATV.dial :AIV F*; I +43 h I F*x% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial PSR P%2 +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%_' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%[' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*| n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F* % h % ."MFBPCR"\AS.seek 0.041ml F*g'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%.' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%> +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%t +113 h P%%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%P' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%J +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %E'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %-' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*W I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *Y'V +849 h ' .t\PSV.dial :air P%h  +113 h  .t\PS.seek 2.5ml P%v' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %\\' +5106 h ' .t\PSV.dial :air P%`  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*N  +43 h  F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *o'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*f I +43 h I F*F% h %Q ."MFBPCR"\AS.seek 0.01ml F*A' +171 h ' .t\PSV.dial :mixloop P%D +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb % ' +619 h ' ."MFBPCR"\ATV.dial :waste F*c n +129 h n ."MFBPCR"\AS.seek :empty F*)'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*w  +86 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *a' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F*Q% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'z% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'R  .t\CTV.dial CSV C'(  +38 h  +113  .t\CBV.dial :waste1 C'e @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*r  +22 h ."MFBPCR"delay 3 seconds +300 F*m% h %_ ."MFBPCR"\AS.seek 0.434ml F*/'ݏ +110 h '4 .t\CSV.dial CSR C'B  +38 h  C'5% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*y n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'K' +1625 h ' ."connect"\CSV.dial CTV C'!  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C',& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +7 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&#& h &ӷ M&M% h % .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &7(- +264 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'@& h & M&]% h %\  A(<% h %X A(?. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (s(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (p& h &,9 M&& h &- M&}% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &X(w +166 h (w M&& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(& h &,9 ."WCR"\SE.seek 17.71 Pucks below Top Plate A(k'* +86 h '* A(& h &,9 M&9& h &w ."WCR"\Elbow.seek 4 M&0(w +6 h (w M&& h &w A(q& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&''7 +23 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&i& A(^'X h &w M&;% h %7 ."WCR"\Forearm.seek :retract M&x'\ +18 h '&0 ."MFBPCR"delay 3 seconds +68 h 'X ."downElevator"\SE.coast A(5 +5 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +142 h (ӷ M&n& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +85 ."MFBPCR"\ATV.dial :waste2 F*2 % +6 h ' ."WCR"\CC.seek :closed C'*(W +37 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +379 h (W ."WCR"Hand.open M&1 +150 h 1 M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +30 h 'R ."MFBPCR"\ATV.dial :air2 F*|  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b * ' +39 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)% h % C'[& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'>  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.seek 0.255ml F*S'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*s% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*% n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*t  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*. @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*g I +86 h I ."MFBPCR"\AS.seek 0ml F*+'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*J'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *W'\  +1032 h '\  F*d% h %\  ."MFBPCR"\AS.seek 0.5ml F*h'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*=% h %(A ."MFBPCR"\AS.seek 0.6ml F*v'\  +1031 h '\  F*`% h %\  ."MFBPCR"\AS.seek 0.5ml F*\\'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*N% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *-' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +3472 h 'V ."MFBPCR"\SPE.coast F*f  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1317h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*F  +64 h  ."MFBPCR"\ARV.dial :carrier F*D  +11 h  F*o% h %V ."MFBPCR"\AS.seek 0.5ml F*O'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*A  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +21 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*a  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*b  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*~' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*e % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +107 h  ."MFBPCR"delay 7 seconds +700 F*B% h %< ."MFBPCR"\AS.seek 0.594ml F*5'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*r n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*!% h % \\ ."MFBPCR"\AS.seek 0.326ml F*K'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226047 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'1  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C', @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'G @ +75 h @ +50 S)4% h % ."WCR"\CBV.dial between CSV and waste2b C'{  +95 h  C'#% h % ."WCR"\CSV.dial between air and CTV C'H ` +18 h ` ."WCR"\CS.seek 7.288ml C'u' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  'M  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'j'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'l' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'"  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'$  +37 h  C']% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'?' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'p' +815 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'@ @ +76 h @ +50 S)<% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'9  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '7' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'q  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'X @ +75 h @ +50 S)Z% h % ."WCR"\CBV.dial between CSV and waste2b C';  +94 h  C'U% h % ."WCR"\CSV.dial between air and CTV C'k ` +57 h ` ."WCR"\CS.seek 7.288ml C'n' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'^  '2  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C''  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'V' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +150 h C'x& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&_% h %\  A(|% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ([( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (>& h & M&& h &ӷ M&8% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &s( +143 h ( M&S& h & A(%& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &t' +89 h ' A(& h & ."WCR"\SE.seek 1.71 Pucks below Top Plate A(&': +244 h ': M&& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &g' +26 h ' ."WCR"\Elbow.hold M&P, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(+- M&$&.,(\ #b h -+: ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +27 h ' M&E% h % A(J& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&d'B +20 h 'B M&W& h & A(=& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &h' +23 h ' ."WCR"\SE.seek 3.04 Pucks below Top Plate A(`' +36 h ' M&v& h & M&N% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&-& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ ."WCR"\Forearm.seek CC M&F' +91 h ' ."WCR"Hand.close M&D/ +150 h / ."WCR"\CC.seek :open C'o( +416 h ( A(Y& h & A(O& h & M& & h &ӷ M&c% h % ."WCR"\Forearm.seek :retract M&w'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &A( +143 h ( M&6& h & A(Q& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b & ' +92 h ' ."WCR"\Elbow.hold M&a, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &z1 +150 h 1 M&)% h % A(b& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&R'B +20 h 'B M&T& h & A(e& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(~% h % ."downElevator"\SE.seek :down A(m'X +100 (B% h %1 M&(% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&51 +2 A(6 h 1 M&\ & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&y& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +264 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &G' +91 h ' .t\CC.seek :closed C'4(W +416 h (W C'{& h &W .tHand.open M +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'M& h &W C':% h % .t\CSV.dial CSR C'u  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'j'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '"% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '$'[ +462 h '[ .t\CSV.dial :waste C'] +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'?& h &W C'@% h %4 .t\CSV.dial :flush C'<  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '}'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'p'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'9'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'q% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +37 h @ C' % h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Z  +38 h  .t\CBV.dial :waste2b C';  +75 h  .t\CS.seek 0ml C'U'4 +766 h '4 #t\Flushing upper manifold C'k& h &W C'i% h %4 .t\CSV.dial :flush C'2  +75 h  .t\CS.seek 5ml C'^'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +37 h @ C''% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I 'x  +38 h +75 .t\CTV.dial :air C'_  +151 h .t\CS.seek 0ml C'|'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'> +38 h .t\CS.seek 0ml C''4 +506 h '4 C'8& h &W C'S% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +506 h '0 .t\CSV.dial :waste C'E +75 h .t\CS.seek 0ml C'J'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'W& h &W C'=% h %4 .t\CSV.dial CSR C'v  +151 h  C'N% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C't'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C's' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& +113 h .t\CTV.dial :intake C'g  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'-' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'f  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'd'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'h% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'P'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\\  '*  +38 h  +113  .t\CBV.dial :waste1 C'Y @ +37 h @ .t\CS.seek 0ml C'O'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'c% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '6'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'F  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C')  +37 h  C'b% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'T'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'~  'w +37 h +76 .t\CTV.dial :air C'A  +150 h .t\CS.seek 0ml C'B'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'5'4 +507 h '4 #t\Flushing upper manifold C'\ & h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'R'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'/% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +507 h '0 .t\CSV.dial :waste C'a +37 h .t\CS.seek 0ml C'!'4 +507 h '4 C'y& h &W C'% h %4 .t\CSV.dial :flush C'r  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C', @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +507 h '0 .t\CSV.dial :waste C'{ +75 h .t\CS.seek 0ml C'#'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'H& h &W C'M% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 5ml C'u'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'G% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV C'j  +38 h  +113  .t\CBV.dial :waste1 C'] @ +37 h @ .t\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'?& h &W C'@% h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C'l'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'p& h &W C'% h %4 .t\CSV.dial CSR C'q  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'9'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'7' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'Z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C';' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'X  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +606 h '4 .t\CSV.dial CSR C'i  +113 h  C'2% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'U'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +1372 h ' .t\CSV.dial CBV C'' +113 h .t\CS.seek 0ml C'^'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&|' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'( +416 h ( M&8& h &ӷ M&S% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&3& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&W1 +2 h 1 M&=& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &v(- +6 h (- M&N& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.seek :garage M&t'η +91 h 'η .tHand.close M&&/ +150 h / M&g& h &- M&s% h %η .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &-(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &d' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'\\& h &W .tHand.open M&*1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'c% h %4 .t\CSV.dial CSR C'O  +112 h  C'6% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'F'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Q'[ +463 h '[ .t\CSV.dial :waste C') +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2009 h '4 #t\Purging external Intake C'z& h &W C'T% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A +75 h .t\CTV.dial :intake C'e  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'B'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'(& h &W C'\ % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +37 h @ C'`% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'm +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'[& h &W C'y% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'r'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C',% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'{'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&o& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'G( +416 h ( M&u& h &ӷ M&"% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &](- +264 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &@'η +91 h 'η .tHand.open M&<1 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST14-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:17:25CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST14-Oct-18" +263647 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1317h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *p'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*q% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +65 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*8% h % ."MFBPCR"\AS.seek 0.81ml F*S'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1319h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*h% h %V ."MFBPCR"\AS.seek 0.5ml F*v'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\\'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*6  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*d% h %Fj ."MFBPCR"\AS.seek 0.524ml F*^' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*)' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Q  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*z% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +108 h  ."MFBPCR"delay 7 seconds +700 F*b% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +22 h n ."MFBPCR"delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*B  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*5  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*/ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1319h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*[ % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *-'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +31 h  ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*L'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*K% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1321h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +10 h  F*k% h %V ."MFBPCR"\AS.seek 0.5ml F*U'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*>  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*8 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*3 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.53ml F*I' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*+% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*= n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*s  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*E % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1321h25ml.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*\\ % +87 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +21 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +21 h F*^% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*~% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*B % +87 h % F*5% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*[% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1539468020.71 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1400h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*o  +11 h  F*-% h %V ."MFBPCR"\AS.seek 0.5ml F*D'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*u n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*?  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*H  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *V'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*j I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*$' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*L' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*l% h %< ."MFBPCR"\AS.seek 0.594ml F*q'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*9'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*Z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*X  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*;% h % \\ ."MFBPCR"\AS.seek 0.326ml F*7'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*2  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*k % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1400h25ml.pcr F*i% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*n  +22 h ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *>'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F*|'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*I% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F*J % +86 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*h'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*\\% h %m ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1402h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*Q  +11 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*T'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*w n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*B  +21 h ."MFBPCR"delay 2 seconds +200 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*`' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*. % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +22 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*r'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*M% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*- % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1402h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*: @@T ."MFBPCR"\ASV.dial :SPEdelivery F*D  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*P  +151 h F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *?' +423 h ' ."MFBPCR"\ATV.dial :ASV F*H n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*<  +43 h  F*#% h % ."MFBPCR"\AS.seek 0.62ml F*j'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*@ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *V'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*K'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*$ % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *}' +423 h ' ."MFBPCR"\ATV.dial :air3 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*p' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*9% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*X n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*;% h % zG ."MFBPCR"\AS.seek 0.365ml F*Z' +7161 h ' ."MFBPCR"\ATV.dial :waste F*2 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*i % +43 h % F*7% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*x% h % ."MFBPCR"\AS.seek 0.579ml F*U' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h %  ."MFBPCR"\AS.seek 0.604ml F*>'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*3 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*S  +22 h  ."MFBPCR"\AS.seek 0ml F*4'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*I % +108 h % F*|% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*%% h % ."MFBPCR"\AS.seek 0.58ml F*=' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h % q ."MFBPCR"\AS.seek 0.605ml F*&'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +21 h F*s% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*h  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *f' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\\ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +202 h 'R ."MFBPCR"\SPE.coast F*c  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F**'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*d  +42 h  F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *6' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*F I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*z  +43 h  F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*b  +64 h  F*)% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *T'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*w n +65 h n F*e% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *A' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*(  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*\  +64 h  F*.% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*` n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *[' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*/ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*y  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*! n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*a n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*,  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*r % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*o'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*- % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*"% h % ."MFBPCR"\AS.seek 0.97ml F*u'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *#'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *V'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*$% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *K' +9988 h ' ."MFBPCR"\ATV.dial AIV F*L I +43 h I ."MFBPCR"\AS.seek 0.02ml F*}' +9880 h ' ."MFBPCR"\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*q  +21 h ."MFBPCR"\ATV.dial :air3 F*p % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *9'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*X% h %Z ."MFBPCR"\AS.seek 0.81ml F*;'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*7% h %n ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*'  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +64 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +21 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*Y % +108 h % ."MFBPCR"\AS.seek 0ml F* 'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990256 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )d h  C' 'O h  P% %* h  F* *F h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *6  h  .t\SPE.configure SPEconfig F*\ 2 *^% h %V .t\AS.configure ASconfig S)z% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'~& S)#.0X 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:18:25CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #17) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'"& h & .tHand.open M&1 +150 h 1 M&H& h &M M&u% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &<(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &@'η +91 h 'η .tHand.close M&j/ +150 h / M&& h &- M&$% h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'}& h &W .tHand.open M&1 +150 h 1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'% h %4 .t\CSV.dial CSR C'  +150 h  C'X% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '9'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '2'[ +463 h '[ .t\CSV.dial :waste C'; +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +2009 h '4 #t\Purging external Intake C'7& h &W C'Z% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'n 'k  h +75 .t\CTV.dial :intake C'1  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'_'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'8& h &W C'% h %4 .t\CSV.dial :flush C'>  +75 h  .t\CS.seek 5ml C'S'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'4% h %p .t\CS.seek 7ml C'3'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I +113 h .t\CTV.dial :air .\CS.seek 0ml C'|'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'%'4 +507 h '4 C'& h &W C'N% h %4 .t\CSV.dial :flush C'W  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'J @ +38 h @ C'=% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'&'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'g +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +7 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.seek CC M&\\' +91 h ' .tHand.close M&f/ +150 h / .t\CC.seek :open C'c( +416 h ( M&& h &ӷ M&Y% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &G(- +264 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&C1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:18:31CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:18:25CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F* % #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C':& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&d% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(O% h %X A(*. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (F(,9 +6 F*6% h %&K ."readyMFB"\ATV.dial :waste2 F*^ % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *z' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (~& h &,9 M&& h &- M&b% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &)(w +166 h (w M&Q& h &w A(w& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &e' +89 h ' A(T& h &,9 .t\SE.seek 16.71 Pucks below Top Plate A('; +96 h '; A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&((w +6 h (w M&& h &w A(\ & h &,9 .t\Forearm.seek 4 + 300 counts M&.'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &`/ +150 h / ."downElevator"\SE.seek :down M&& A(5'X h &w M&% h %7 .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.seek CC M&a(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'-(W +416 h (W .tHand.open M&D1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +6 h + S)% h % C'"& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.seek 0ml C'_  S)0' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)4  +144 h  +7  S)S% h % .t\CBV.dial between SSV and waste2 C'I  +18 h  C'3% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'|  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '+'4 +2610 h '4 C'% h %4 S)% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'N @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'9 h 9Td +100 C'19 h 9kd +100 C'>9 h 9d +100 C'_9 h 9d +100 C'49 h 9d +100 C'S9 h 9d +100 C'I9 h 9d +100 C'39 h 9d +100 C'|9 h 9 d +100 C'i9 h 9 d +100 C'89 h 96d +100 C'9 h 9Ld +100 C'9 h 9bd +100 C'N9 h 9xd +100 C'09 h 9d +100 C'%9 h 9d +100 C'J9 h 9d +100 C'=9 h 9d +100 C'W9 h 9d +100 C'9 h 9d +100 C'&9 h 9d +100 C'g9 h 9(d +100 C'9 h 9>d +100 C's9 h 9Td +100 C'v9 h 9id +100 C'+9 h 9d +100 C'h9 h 9d +100 C't9 h 9d +100 C'f9 h 9d +100 C'p9 h 9d +100 C'9 h 9d +100 C'Y9 h 9  d +100 C']9 h 9 d +100 C'9 h 9 *d +100 C'\\9 h 9 ?d +100 C'9 h 9 Td +100 C'C9 h 9 j d +100 C'9 h 9 $d +100 C'E9 h 9 (d +100 C'9 h 9 ,d +100 C' 9 h 9 0d +100 C'd9 h 9 4d +100 C'O9 h 9 8d +100 C'*9 h 9 'B +20 h 'B M&_& h & A(4& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &S' +23 h ' .t\SE.seek 4.04 Pucks below Top Plate A(I' +36 h ' M&3& h & M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +89 h '\  .t\Elbow.seek CC M&8(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&N' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'%( +416 h ( A(J& h & A(=& h & M&W& h &ӷ M&% h % .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &g( +142 h ( M&& h & A(s& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &v' +93 h ' .t\Elbow.hold M&+, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &h1 +150 h 1 M&t% h % A(f& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&p'B +20 h 'B M&& h & A(Y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &]' +22 h ' A(% h % ."downElevator"\SE.seek :down A(\\'X +100 (% h %  M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'E @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'd { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'O& h & .tHand.open M&*1 +2 h 1 M&6& h & M&^% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&:/ +150 h / M&Q& h &- M&w% h %η .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +264 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C'A& h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%\ +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%)' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%M +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%.  C'  h +75 .t\PSV.dial :mixloop P%( +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%[  h .t\PS.seek 0ml P%5' +2610 h ' .t\PSV.dial :air P%D  +38 h  .t\PS.seek 2.5ml P%c' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%"' +2610 h ' .t\PSV.dial :air P%R  +37 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.seek 0ml P%r' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&!& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.seek CC M&z' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'/( +416 h ( M&$& h &ӷ M&% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&P1 +150 h 1 M&u% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'q& h & M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (}(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (l& h &,9 M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +166 h (w M&2& h &w A(Z& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &a' +89 h ' A( & h &,9 .t\SE.seek 15.71 Pucks below Top Plate A(X'L +107 h 'L A(k& h &,9 M&x& h &w .t\Elbow.seek 4 M&(w +7 h (w M&1& h &w A(_& h &,9 .t\Forearm.seek 4 + 300 counts M&4'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &3/ +150 h / ."downElevator"\SE.seek :down A(|'X M&7& h &w M&9% h %7 .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.seek CC M&n(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&S1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'i  +151 h  .t\CSV.dial CTV C'-  +18 h  .t\CS.seek 5ml C'J'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'=' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'W'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%s +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%N  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%8 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%h  +151 h  .t\PS.seek :empty P%t' +2610 h ' .t\CBV.dial :waste1 C'f @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'Y @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'& ` +19 h ` .t\CSV.dial :air C'g @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'C  +37 h  .t\CS.seek 0ml C'E'4 +2648 h '4 .t\CBV.dial :waste1 C'v @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'd& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&O% h %\  A(*% h %X A(6. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (^( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (p& h & M&I& h &ӷ M&]% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &,( +143 h ( M&~& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\\' +89 h ' A(@& h & .t\SE.seek 3.71 Pucks below Top Plate A(+' +224 h ' M&:& h & A(Q& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &w' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b .t\SE.seek 4.04 Pucks below Top Plate A(G' +27 h ' M&B% h % A( & h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&T'B +21 h 'B M&%& h & A(A& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 5.04 Pucks below Top Plate A( ' +36 h ' M&\ & h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&e(ӷ +142 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C'( +416 h ( A((& h & A([& h & M&y& h &ӷ M&D% h % .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&c& h & A(R& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &"' +92 h ' .t\Elbow.hold M&H, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &F1 +150 h 1 M&!% h % A(#& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&?'B +21 h 'B M&& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &`' +22 h ' A({% h % ."downElevator"\SE.seek :down A($'X +100 (% h % M&z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +22 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%<  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%P' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%u +37 h .t\PRV.dial :air P%j  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%q  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%l% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %L' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'2& h & .tHand.open M&Z1 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&k(- +165 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&V/ +150 h / M&3& h &- M&7% h %η .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +265 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'X(W +416 h (W C'4& h &W .tHand.open M&1 +150 h 1 M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  C'/& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%S @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%U  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%i  +75 h  .tdelay 2 seconds +200 P%-% h % .t\PS.seek 1.499ml P%>' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%}' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%J' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%= +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%r% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %s'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%N  +75 h .tdelay 2 seconds +200 P%8% h %r .t\PS.seek 0.475ml P%h' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%f'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%Y  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%& +76 h .t\PTV.dial :PRV,avoiding: :puck P%g @ +75 h @ .t\PS.seek 0ml P%W' +2610 h ' .t\PSV.dial :air P%C  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%v +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%d  +75 h  .t\PS.seek 2.5ml P%O' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%* +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%6  +76 h P%p% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %I' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'] & +50 h & .t\AWV.select :waste C' +6 P%~% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'@ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %n' +1313 h ' .t\PSV.dial :air P%0  +38 h  .t\PS.seek 2.5ml P%:' +2610 h ' .t\PSV.dial :PML P%Q +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'B& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C' & ."MFBPCR"\ASV.dial :SPEdelivery F*% @@T h &W F*A  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%\ +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%\\' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*, I +129 h I F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *w'6 +66 .t\PSV.dial :waste1 P%)  +37 h  .t\PS.seek 0ml P%M' +1598 h ' .t\PSV.dial PSR P%T +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%G' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*.  +22 h ' .t\PSV.dial PSR P% +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*('ͯ +92 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%y' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*D % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%R  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*F% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *!' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%# +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*b I +43 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*5' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%z +113 h P%"% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%?' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%<' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*` n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*$% h % ."MFBPCR"\AS.seek 0.041ml F*^'% +336 h ' .t\PSV.dial PSR P%t +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%u' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%j +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%l  +37 h  .t\PS.seek 0ml P%P' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%Z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %x' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*1 I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *H'V +849 h ' .t\PSV.dial :air P%V  +113 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :mixloop P%7 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %K' +5107 h ' .t\PSV.dial :air P%_  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %a' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *9'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*4% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%| +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %/' +618 h ' ."MFBPCR"\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*U  +86 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *-' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*L  +43 h F*>% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'}  +37 h  C'=% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'r' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'J  'N  +38 h  +113  .t\CBV.dial :waste1 C'8 @ +37 h @ .t\CS.seek 0ml C's'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*h% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'Y  +37 h  C'f% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'&'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*g  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*C'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*W n +108 h n F*v% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'd' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'*'4 +2647 h '4 C'6& h &W #tGaraging FlushPuck in Collection Clamp M&p& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +7 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.seek CC M&I' +90 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +416 h ( M&0& h &ӷ M&n% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +265 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &A'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C',& h & M&+% h %\  A()% h %X A(m. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (\\(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (T& h &,9 M&M& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&.& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A([& h &,9 ."WCR"\SE.seek 14.71 Pucks below Top Plate A(w'] +116 h '] A(& h &,9 M&D& h &w ."WCR"\Elbow.seek 4 M&((w +7 h (w M&R& h &w A(e& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +138 h '&0 ."MFBPCR"delay 3 seconds +12 h / ."downElevator"\SE.seek :down M&#& A(c'X h &w M&b% h %7 ."WCR"\Forearm.seek :retract M&{'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&!& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +55 ."MFBPCR"\ATV.dial :waste2 F*z % +36 h ' ."WCR"\CC.seek :closed C'"(W +7 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +409 h (W ."WCR"Hand.open M& 1 +150 h 1 M&?% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ + h 'R ."MFBPCR"\ATV.dial :air2 F*`  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *$' +68 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)5+ +7 h + S)t% h % C'<& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'j  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +21 h  ."MFBPCR"\AS.seek 0.3ml F*-': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*}'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*= % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +86 h F*J% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *N'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*8 I +43 h I F*r% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*h n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*Y  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *f% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3301 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *s% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *g' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*W% h % ."MFBPCR"\AS.seek 0.279ml F*v'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*C n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*d% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *6'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*p I +21 h I F*]% h %v ."MFBPCR"\AS.seek 0.059ml F*~'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*@ % +21 h % F*;% h %H ."MFBPCR"\AS.seek 0.069ml F*'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F*0% h % ."MFBPCR"\AS.seek 0.769ml F*n' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +21 h % ."MFBPCR"delay 3 seconds +300 F*B% h % q ."MFBPCR"\AS.seek 0.794ml F* ' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +21 h F*\ % h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*Q I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *,' +4416 h ' ."MFBPCR"\ATV.dial :waste F*+ n +129 h n ."MFBPCR"\AS.seek :empty F*)'R +115 h 'R ."MFBPCR"\ATV.dial :air3 F*m % +43 h % F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *M' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*A% h % ."MFBPCR"\AS.seek 0.579ml F*.' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %  ."MFBPCR"\AS.seek 0.604ml F*:'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*D% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*R' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +42 h  ."MFBPCR"\AS.seek 0ml F*y'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *b'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*G % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*!' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*F% h %  ."MFBPCR"\AS.seek 0.09ml F*w' +10076 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*( I +86 h I F*c% h % ."MFBPCR"\AS.seek 0.12ml F*{' +626 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*`% h % ."MFBPCR"\AS.seek 0.23ml F*t' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\\% h %X ."MFBPCR"\AS.seek 0.24ml F*5' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*u @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*^ I +86 h I ."MFBPCR"\AS.seek 0ml F*Z'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *1'\  +1032 h '\  F*2% h %\  ."MFBPCR"\AS.seek 0.5ml F*V'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*3'\  +1032 h '\  F*_% h %\  ."MFBPCR"\AS.seek 0.5ml F*K'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *x' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*7 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +3473 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1417h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*4  +64 h  ."MFBPCR"\ARV.dial :carrier F*|  +10 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*$ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*-  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*=% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*8 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.536ml F*N'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*h  +107 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*C  +65 h ."MFBPCR"\ARV.dial :air1 F*v  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*d'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226077 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C']  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'6 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'; @ +76 h @ +50 S)~% h % ."WCR"\CBV.dial between CSV and waste2b C'X  +94 h  C'0% h % ."WCR"\CSV.dial between air and CTV C'' ` +19 h ` ."WCR"\CS.seek 7.288ml C'I' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'B  'n  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'O'4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'\  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'Q  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'+% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'm' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C', @ +75 h @ +50 S))% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'A% h % ."WCR"\CSV.dial between air and CTV C'M ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  '.  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'D  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ':' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'e  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'R% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'y' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '#' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'G @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'b  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'F ` +57 h ` ."WCR"\CS.seek 7.288ml C'!' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'z  '(  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'c'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'w  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&?% h %\  A(`% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (<( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (j& h & M&t& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &\\( +142 h ( M&& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(5& h & ."WCR"\SE.seek 4.71 Pucks below Top Plate A(l' +214 h ' M&q& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &^' +25 h ' ."WCR"\Elbow.hold M&P, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(Z- h -+ ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +27 h ' M& % h % A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&2'B +21 h 'B M&1& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &V' +22 h ' ."WCR"\SE.seek 6.04 Pucks below Top Plate A(_' +36 h ' M&3& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +90 h '\  ."WCR"\Elbow.seek CC M&7(ӷ +142 h (ӷ M&x& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&4' +91 h ' ."WCR"Hand.close M&|/ +150 h / ."WCR"\CC.seek :open C'9( +416 h ( A(H& h & A(k& h & M&$& h &ӷ M&S% h % ."WCR"\Forearm.seek :retract M&U'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&L& h & A(>& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &i' +92 h ' ."WCR"\Elbow.hold M&-, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &=1 +150 h 1 M&% h % A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&J'B +21 h 'B M&}& h & A(8& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &r' +22 h ' A(E% h % ."downElevator"\SE.seek :down A(h'X +100 (Y% h % M&N% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C's& h & .tHand.open M&f1 + A(6 + h 1 M&& h & M&g% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&v'η +91 h 'η .tHand.close M& / +150 h / M&W& h &- M&&% h %η .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +264 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C'~(W +416 h (W C'X& h &W .tHand.open M&01 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'n& h &W C'B% h % .t\CSV.dial CSR C'I  +38 h  C'\ % h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'O'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'Q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'+ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'm& h &W C',% h %4 .t\CSV.dial :flush C')  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +37 h @ C'M% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV C'[  .t\CTV.dial CSV C'.  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'e% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'R'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C': @ +37 h @ C'y% h %p .t\CS.seek 7ml C'G'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'b  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'F& h &W C'#% h %4 .t\CSV.dial :flush C'(  +75 h  .t\CS.seek 5ml C'z'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +37 h @ C'w% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '{  +38 h +75 .t\CTV.dial :air C'?  +151 h .t\CS.seek 0ml C'`'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'j +38 h .t\CS.seek 0ml C't'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'5'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'1& h &W C'% h %4 .t\CSV.dial CSR C'3  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C' 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'\\' +416 h ' ."connect"\CSV.dial CBV C'l .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'^  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '2'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'V% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'P'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'K  '"  +38 h  +113  .t\CBV.dial :waste1 C'H @ +37 h @ .t\CS.seek 0ml C'k'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'$  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'L'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '>' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  +38 h  .t\CBV.dial :waste2b C'7  +75 h  .t\CS.seek 0ml C'='4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +710 h ' ."connect"\CSV.dial CBV C'E .t\CBV.dial CSV C'U  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'Y'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'N'0 +506 h '0 .t\CSV.dial :waste C's +38 h .t\CS.seek 0ml C'f'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'g% h %4 .t\CSV.dial :flush C'i  +37 h  .t\CS.seek 5ml C'J'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +37 h @ C'% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r +113 h .t\CTV.dial :air .\CS.seek 0ml C'h'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'- +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'W& h &W C'&% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'v'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'*% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'p'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'X +76 h .t\CS.seek 0ml C'0'4 +506 h '4 #t\Flushing lower manifold's waste1 port C''& h &W C'n% h %4 .t\CSV.dial :flush C'B  +38 h  .t\CS.seek 5ml C'I'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C';% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'O  'Q  +38 h  +112  .t\CBV.dial :waste1 C'+ @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'm& h &W C',% h %4 .t\CSV.dial :flush C')  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'A @ +37 h @ C'M% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'[  +38 h  .t\CBV.dial :waste2b C'.  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'T& h &W C'% h %4 .t\CSV.dial CSR C'e  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'D'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C':' +416 h ' ."connect"\CSV.dial CBV C'y .t\CBV.dial CSV C'R  +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'b' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'G  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'F'4 +607 h '4 .t\CSV.dial CSR C'#  +113 h  C'(% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +1372 h ' .t\CSV.dial CBV C'w +113 h .t\CS.seek 0ml C'z'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.seek CC M&`' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C' ( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&q1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&11 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&l/ +150 h / M&^& h &- M&\\% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'K& h &W .tHand.open M&"1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'$& h &W C'S% h %4 .t\CSV.dial CSR C'k  +113 h  C'L% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'4'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '7% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '>'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'=& h &W C'}% h %4 .t\CSV.dial :flush C'U  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'8  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'Y'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C's  +75 h #t\Flushing upper manifold C'N& h &W C'% h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C'i'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'_% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'J +113 h .t\CTV.dial :air .\CS.seek 0ml C'r'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'h +37 h .t\CS.seek 0ml C'-'4 +507 h '4 C'<& h &W C'W% h %4 .t\CSV.dial :flush C'&  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'6% h %0 .t\CS.seek 5ml C'*'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'v'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'p +76 h .t\CS.seek 0ml C'X'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&9& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +7 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.seek CC M&0' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C';( +416 h ( M&I& h &ӷ M&O% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &+(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &,'η +91 h 'η .tHand.open M&)1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST15-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:130\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:20:15CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST15-Oct-18" +263677 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1417h25ml.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*[  +21 h ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *T'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*:'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*b  +65 h  F*#% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*c  +32 h  ."MFBPCR"\ATV.dial :air3 F*j % +86 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1419h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*V% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *k'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*L  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*z' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*7  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*>  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*}'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*U  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +21 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*s'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*N  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1419h25ml.pcr F*r% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*C  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *&'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*B  +65 h  F*\ % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *x'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F*)% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*%'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1421h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*(  +11 h  F*F% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*j  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +22 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *~'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*@% h %Fj ."MFBPCR"\AS.seek 0.524ml F*`' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*q % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*Z% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*5  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*^' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1421h25ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +86 h % F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *$'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*"% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*7'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*z% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*U% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +21 h F*J% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V @CEST1539554420.12 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1500h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*9  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*|'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +21 h ."MFBPCR"delay 2 seconds +200 F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*Q I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*M n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*%' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*.  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*T n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1500h25ml.pcr F*#% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*!  +21 h ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *j'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*?% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*`'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*l  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F*V'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*K% h %m ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1502h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*g I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*J% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*u % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*i'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*h  +21 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*-'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.65ml F*p' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*n% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1502h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*B @@T ."MFBPCR"\ASV.dial :SPEdelivery F*|  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*P  +150 h F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *m' +424 h ' ."MFBPCR"\ATV.dial :ASV F*' n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*)  +43 h  F*0% h % ."MFBPCR"\AS.seek 0.62ml F*Q'\ +2034 h '\  ."MFBPCR"\ATV.dial :waste F*, n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *M'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*d'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h % ."MFBPCR"\AS.seek 0.71ml F*T' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*D% h % Ű ."MFBPCR"\AS.seek 0.72ml F*R' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*G n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*b% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7162 h ' ."MFBPCR"\ATV.dial :waste F*( n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *F'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*# % +42 h % F*:% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +21 h F*{% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %  ."MFBPCR"\AS.seek 0.604ml F*j'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*@% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*c' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*~'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*`% h %V ."MFBPCR"\AS.seek 0.01ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +21 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h % q ."MFBPCR"\AS.seek 0.605ml F*l'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\\% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*5' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*^  +21 h  ."MFBPCR"\AS.seek 0ml F*t'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*V  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +201 h 'R ."MFBPCR"\SPE.coast F*S  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*$ n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*2  +43 h  F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *L' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*4 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*=  +43 h  F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *>': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *}'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*E n +64 h n F*8% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*N  +43 h  F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*u% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *r'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*_ n +64 h n F*C% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*W  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *i': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *&'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*- n +151 h n ."MFBPCR"\AS.seek 0ml F**'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*6  +85 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *v' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +21 h F*p% h % ."MFBPCR"\AS.seek 0.96ml F*9'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*|% h %0 ."MFBPCR"\AS.seek 0.985ml F*\ ' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +21 h F*O% h % ."MFBPCR"\AS.seek 0.97ml F*I'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*' I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *0'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *,'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*M  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *d' +9988 h ' ."MFBPCR"\ATV.dial AIV F*% I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*['V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*e  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *]'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*c % +86 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*1% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*H % +107 h % ."MFBPCR"\AS.seek 0ml F*$'V +2548 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990315 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )2 h  C' 'k h  P% %" h  F* *4 h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *L  h  .t\SPE.configure SPEconfig F*\ 2 *z% h %V .t\AS.configure ASconfig S)=% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'U& S)#.0X% h %\  .t\Forearm.configure LooseForearm M&#Q@2^d^2b &E& h &- .t\Elbow.configure LooseElbow M&$ &.,(#b A(8& h & .t\SC.configure SCloose A($.(\ 1~b (}% h %X .t\SE.configure SEconfig .\CH.coast C's  A(#QK-0-\ b h  .t\CH.configure CHconfig C'\ 2 .t\PH.coast P%  h  .t\PH.configure PHconfig P%\ 2 +29 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +6 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'Y% P%N% h %p ."rdySampler"Intake.select :close h %q M&g% ."rdySampler"\Exhaust.select :close ."rdyProcessing"\PSV.dial :air ."rdySampler"\CTV.dial :air h %= C'  P%f  M&u% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &J'\ h ."rdySampler"\CSV.dial :waste1 C'_ h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)C h  S)r  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M& 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:21:15CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #18) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'O& h & .tHand.open M&\ 1 +150 h 1 M&'& h &M M&I% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &)(- +166 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &,'η +91 h 'η .tHand.close M&Q/ +150 h / M&M& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +264 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&[1 +150 h 1 M&e% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'y& h &W C'% h %4 .t\CSV.dial CSR C'  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'R% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '('[ +463 h '[ .t\CSV.dial :waste C'b +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +2009 h '4 #t\Purging external Intake C':& h &W C'% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'! .t\CBV.dial CSV C'F  h +75 .t\CTV.dial :intake C']  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'?'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'@% h %4 .t\CSV.dial :flush C'j  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +37 h @ C'~% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'`'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'Z +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'\\'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'l'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'^ +75 h .t\CS.seek 0ml C'5'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&t& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&K' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'S( +416 h ( M& & h &ӷ M&H% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &;(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &$'η +91 h 'η .tHand.open M&o1 +150 h 1 M&/% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:21:21CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:21:15CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*a% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'B& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&2% h %\  A(k% h %X A(". h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (4(,9 +6 F*L% h %&K ."readyMFB"\ATV.dial :waste2 F*z % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *=' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (U& h &,9 M&7& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&>& h &w A(E& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &8' +89 h ' A(}& h &,9 .t\SE.seek 13.71 Pucks below Top Plate A(s'n +125 h 'n A(& h &,9 M&Y& h &w .t\Elbow.seek 4 M&N(w +7 h (w M&g& h &w A(u& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &_/ +150 h / ."downElevator"\SE.seek :down A(C'X M&f& h &w M&r% h %7 .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.seek CC M&-(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&W& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'x(W +416 h (W .tHand.open M&|1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)*% C'O'4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'E' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '}% h %  .t\CS.seek 4.5ml C';'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .t\CS.seek 1.275ml C'Y' +919 h ' #tHeating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'g   'u9 h 9d +100 C'X9 h 9d +100 C'&9 h 91d +100 C'_9 h 9J d +100 C'f9 h 9bd +100 C'r9 h 9zd +100 C'9 h 9d +100 C'89 h 9d +100 C'W9 h 9 d +100 C'n9 h 9$d +100 C'9 h 9(d +100 C's9 h 9 ,d +100 C'N9 h 9$0d +100 C'C9 h 9<4d +100 C'<9 h 9S8d +100 C'S9 h 9kd +100 C')9 h 9Td +100 C'9 h 9kd +100 C'P9 h 9d +100 C'9 h 9d +100 C'[9 h 9d +100 C'9 h 9d +100 C'I9 h 9d +100 C'y9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'd9 h 96d +100 C' 9 h 9Ld +100 C'm9 h 9bd +100 C'R9 h 9xd +100 C'D9 h 9d +100 C'b9 h 9d +100 C'G9 h 9d +100 C'(9 h 9d +100 C'9 h 9d +100 C'w9 h 9d +100 C'F9 h 9d +100 C':9 h 9(d +100 C']9 h 9>d +100 C'e9 h 9Td +100 C'!9 h 9id +100 C'#9 h 9d +100 C'{9 h 9d +100 C'9 h 9d +100 C'@9 h 9d +100 C'?9 h 9d +100 C'~9 h 9d +100 C'9 h 9  d +100 C'c9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'j9 h 9 Td +100 C'9 h 9 j d +100 C'Z9 h 9 $d +100 C'q9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 9 h 9 d .t\CH.hold 5:00,85C C'7   P h  +30000  C'}9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'E'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'; @ +37 h @ .t\CS.seek 5ml C'u'p +1373 h 'p .t\CSV.dial CTV C'X  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'& @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'_  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'f @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'r'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'8  +19 h C'W% h %4 .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C's% h %p .t\CS.seek 7ml C'N'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'C @ +37 h @ .t\CS.seek 10ml C'<' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'S ` +132 h ` #tLysing Complete C'|& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&J% h %\  A(% h %X A(*. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ('( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (v& h & M&5& h &ӷ M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&0& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &O' +89 h ' A(& h & .t\SE.seek 5.71 Pucks below Top Plate A(Q' +205 h ' M&M& h & A(=& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &\ ' +25 h ' .t\Elbow.hold M&p, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(,- h -+ .t\SE.seek 6.04 Pucks below Top Plate A(%' +27 h ' M&)% h % A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &P'B +21 h 'B M&& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(I' +36 h ' M&y& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&d(ӷ +142 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&D/ +150 h / .t\CC.seek :open C'b( +416 h ( A(G& h & A((& h & M&& h &ӷ M&w% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &:( +142 h ( M&]& h & A(e& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &!' +93 h ' .t\Elbow.hold M&#, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &{1 +150 h 1 M&% h % A(@& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&?'B +21 h 'B M&~& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (j% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'q @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&\\% h %\  .t\Elbow.seek FlushPuck::Garage M&`(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&t'η +91 h 'η .tHand.close M&1/ +150 h / M&3& h &- M&% h %η .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C'+(W +416 h (W C'& h &W .tHand.open M& 1 +150 h 1 M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%K +151 h P%o% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%a  +38 h  .t\PS.seek 0ml P%/' +1597 h ' .t\PSV.dial PSR P%2 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%B' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'"  P%k  h +75 .t\PSV.dial :mixloop P%L +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%$  h .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P%6' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%>' +2609 h ' .t\PSV.dial :air P%7  +38 h  .t\PS.seek 2.5ml P%}' +2610 h ' .t\PSV.dial :mixloop P%Y +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&;& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +6 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&f/ +150 h / .t\CC.seek :open C'u( +416 h ( M&& h &ӷ M&8% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &n'η +91 h 'η .tHand.open M&C1 +150 h 1 M&g% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'S& h & M&|% h %\  A(J% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (*(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (v& h &,9 M&5& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &0(w +166 h (w M&& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &-' +89 h ' A(M& h &,9 .t\SE.seek 12.71 Pucks below Top Plate A(=' +136 h ' A(i& h &,9 M&,& h &w .t\Elbow.seek 4 M&)(w +7 h (w M&& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&['7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +150 h / ."downElevator"\SE.seek :down M&O& A('X h &w M& % h %7 .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.seek CC M&\ (ӷ +45 h 'X ."downElevator"\SE.coast A(5 +98 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'%(W +415 h (W .tHand.open M&1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'x  +19 h  .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'(' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%w  +38 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :mixloop P%e +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%R  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%d +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%{  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'@ @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'~  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'F ` +18 h ` .t\CSV.dial :air C': @ +38 h @ .t\CS.seek 10ml C'j' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'q'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&% h %\  A(% h %X A(\\. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (!( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (?& h & M&I& h &ӷ M&c% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &^( +142 h ( M& & h & A('& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(<& h & .t\SE.seek 6.71 Pucks below Top Plate A(#' +195 h ' M&1& h & A(3& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&Z, +6 h , .t\Elbow.reconfigure TightElbow A(t- M&$&.,(\ #b h -+ .t\SE.seek 7.04 Pucks below Top Plate A(T' +27 h ' M& % h % A(`& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&.'B +20 h 'B M&b& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' .t\SE.seek 8.04 Pucks below Top Plate A(l'w +37 h 'w M&K& h & M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  .t\Elbow.seek CC M&a(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.seek CC M&/' +90 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'"( +416 h ( A(L& h & A($& h & M&B& h &ӷ M&U% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&6& h & A(7& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &>' +92 h ' .t\Elbow.hold M&Y, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&;% h % A(X& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&&'B +21 h 'B M&f& h & A(V& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &A' +22 h ' A(+% h %w ."downElevator"\SE.seek :down A('X +100 (8% h % M&_% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%s  +113 h  P%E% h % .t\PS.seek 0.499ml P%C' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%g +38 h .t\PRV.dial :air P%W  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%S  +37 h .t\CBV.dial AIV C'|  +38 h .t\PRV.dial between CBV and air P%J  +19 h  .t\PRV.dial :air P%  +19 h  P%v% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %5' +250 h ' .t\PRV.dial :waste2 P%4 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %n' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&M& h & M&r% h %\  .t\Elbow.seek FlushPuck::Garage M&i(- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&N/ +150 h / M&y& h &- M&O% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +264 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'=& h &W .tHand.open M&)1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  C'u& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%p  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%x% h % .t\PS.seek 1.499ml P%P' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%*' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%G' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%( +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%w  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %e'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%R  +76 h .tdelay 2 seconds +200 P%d% h %r .t\PS.seek 0.475ml P%{' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%]  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%@'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%~  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%F +75 h .t\PTV.dial :PRV,avoiding: :puck P%: @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%j' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h P%?% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %I' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'c & +50 h & .t\AWV.select :waste C' +7 P% % h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %''nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'< { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %\ ' +1313 h ' .t\PSV.dial :air P%1  +37 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :PML P%D +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 ' & h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'`& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*b @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%K +21 h  ."MFBPCR"delay 20 seconds +92 h P%o% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*^ I +129 h I F*#% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%2' +1597 h ' .t\PSV.dial PSR P%. +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%T' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Z' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*k  +22 h ' .t\PSV.dial PSR P%H +21 h  F*%% h %6 ."MFBPCR"\AS.seek 0.329ml F*L'ͯ +92 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%B' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*U % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%7  +37 h  .t\PS.seek 0ml P%/' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*a  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *;' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%X +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%f' +217 ."MFBPCR"\ATV.dial :AIV F*V I +42 h I F*+% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial PSR P%_ +113 h P%>% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%&' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%l  +38 h  .t\PS.seek 0ml P%s' +739 h ' ."MFBPCR"delay 5 seconds +500 F*E9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*A n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*!'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%g' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%W +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial PSR P%| +112 h P%J% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%C' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%5' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %M'k +2679 h 'k .t\PSV.dial :waste1 P%r  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %,' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *Y'V +850 h ' .t\PSV.dial :air P%N  +112 h  .t\PS.seek 2.5ml P%y' +2610 h ' .t\PSV.dial :mixloop P%O +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %-' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b * 'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*" I +43 h I F*=% h %Q ."MFBPCR"\AS.seek 0.01ml F*)' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %u' +619 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*m'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*p  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *x' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*n  +43 h F*P% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'*  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  'R  +37 h  +113  .t\CBV.dial :waste1 C'd @ +38 h @ .t\CS.seek 0ml C'e'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*]  +22 h ."MFBPCR"delay 3 seconds +300 F*{% h %_ ."MFBPCR"\AS.seek 0.434ml F*~'ݏ +110 h '4 .t\CSV.dial CSR C'  +38 h  C'@% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'F'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*:  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*j'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'\\& h &W #tGaraging FlushPuck in Collection Clamp M&?& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&I' +91 h ' .tHand.close M&'R +22 h ' ."WCR"\CC.seek :closed C'8(W +416 h (W ."WCR"Hand.open M&l1 +121 h 'R ."MFBPCR"\ATV.dial :air2 F*&  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *E' +7 h 1 M&A% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +7 h + S)% h % C's& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'W  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*|  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*g @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*! I +85 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*4'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*r  +43 h  #"MFBPCR"\Swish #1 of 2 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*N'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*5% h %(A ."MFBPCR"\AS.seek 0.6ml F*y'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F* % h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *,' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*O % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +3472 h 'V ."MFBPCR"\SPE.coast F*"  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1515h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*=  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*i'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*)  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*n  +21 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*x  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*(% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*w  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*q' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*d % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*@'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*] n +22 h n ."MFBPCR"delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226107 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'c  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'\\ @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'0 @ +75 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'[  +94 h  C'1% h % ."WCR"\CSV.dial between air and CTV C'Q ` +19 h ` ."WCR"\CS.seek 7.288ml C'I' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '\  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'K  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '`' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'D  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'o  +38 h  C'#% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'h' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'^ @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'2 ` +57 h ` ."WCR"\CS.seek 7.288ml C'%' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'$  'k  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Z'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'U  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '3' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'a  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C't  +38 h  C'7% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'T @ +75 h @ +50 S)H% h % ."WCR"\CBV.dial between CSV and waste2b C'V  +95 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C';' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'_  'L  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '6'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'}  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'j' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C''  +151 h C'+& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&l% h %\  A(A% h %X A(&. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (s( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (W& h & M&& h &ӷ M&f% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&>& h & A(J& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &|' +90 h ' A(z& h & ."WCR"\SE.seek 7.71 Pucks below Top Plate A(v' +184 h ' M&S& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &!' +26 h ' ."WCR"\Elbow.hold M&C, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+ ."WCR"\SE.seek 8.04 Pucks below Top Plate A(r'w +27 h 'w M&M% h % A(4& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&& h & A(5& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &N' +23 h ' ."WCR"\SE.seek 9.04 Pucks below Top Plate A('f +36 h 'f M&y& h & M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&O(ӷ +143 h (ӷ M&,& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ ."WCR"\Forearm.seek CC M&=' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C' ( +416 h ( A(Y& h & A(i& h & M&E& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&p'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &)( +142 h ( M&n& h & A(P& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&x, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &(1 +150 h 1 M&m% h % A(w& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&G'B +20 h 'B M&*& h & A(d& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(q% h %f ."downElevator"\SE.seek :down A({'X +100 (% h % M&R% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +73 h 'X ."downElevator"\SE.coast A(5 +17 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&@1 +2 h 1 M&& h & M&:% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&~& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&F% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'[& h &W .tHand.open M&11 +150 h 1 M&Q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'\ & h &W C' % h % .t\CSV.dial CSR C'I  +37 h  C'K% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'D% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'o'[ +463 h '[ .t\CSV.dial :waste C'# +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '`'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'h& h &W C'^% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'2% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'k  '$  +38 h  +113  .t\CBV.dial :waste1 C'% @ +75 h @ .t\CS.seek 0ml C'U'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'Z& h &W C'a% h %4 .t\CSV.dial :flush C't  +75 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +38 h @ C'B% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'H  +38 h  .t\CBV.dial :waste2b C'V  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'& h &W C'X% h %4 .t\CSV.dial :flush C'L  +75 h  .t\CS.seek 5ml C'_'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +37 h @ C'}% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' '+  +38 h +75 .t\CTV.dial :air C'l  +151 h .t\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C''4 +506 h '4 C'f& h &W C'>% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'S% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'M +75 h .t\CS.seek 0ml C'4'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'5% h %4 .t\CSV.dial CSR C'y  +150 h  C' % h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'|'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'v +113 h .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C',' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'"  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'r  +113 h  C'N% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'C'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'8  '  +38 h  +113  .t\CBV.dial :waste1 C'Y @ +37 h @ .t\CS.seek 0ml C'i'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'E  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'n'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  +38 h  .t\CBV.dial :waste2b C'O  +75 h  .t\CS.seek 0ml C'('4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'm  +37 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '*'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'd' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'p  'q +37 h +75 .t\CTV.dial :air C')  +151 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'R'0 +506 h '0 .t\CSV.dial :waste C'e +38 h .t\CS.seek 0ml C'@'4 +507 h '4 #t\Flushing upper manifold C'& h &W C':% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'~% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'{'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +506 h '0 .t\CSV.dial :waste C'x +38 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'F% h %4 .t\CSV.dial :flush C']  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\\ @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'?'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'[ +76 h .t\CS.seek 0ml C'1'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'Q& h &W C'\ % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'I'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'0% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  '  +37 h  +113  .t\CBV.dial :waste1 C'# @ +38 h @ .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'h& h &W C'^% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'`'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'2% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'k  +38 h  .t\CBV.dial :waste2b C'$  +75 h  .t\CS.seek 0ml C'%'4 +766 h '4 #tDisinfecting external Intake with kill C'.& h &W C'Z% h %4 .t\CSV.dial CSR C'a  +38 h  C't% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'3' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B '7  +38 h +75 .t\CTV.dial :intake C'H  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'V' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'T  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'X  +113 h  C'L% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C';' +1372 h ' .t\CSV.dial CBV C'} +113 h .t\CS.seek 0ml C'_'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&+& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +7 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&A' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'&( +416 h ( M&f& h &ӷ M&>% h % .t\Forearm.seek :retract M&J'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&5& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &y(- +6 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.seek :garage M&|'η +91 h 'η .tHand.close M&v/ +150 h / M&!& h &- M&% h %η .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +265 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'& h &W .tHand.open M&81 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'% h %4 .t\CSV.dial CSR C'i  +113 h  C'n% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'='p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'O% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'P'[ +463 h '[ .t\CSV.dial :waste C'm +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'w'4 +2009 h '4 #t\Purging external Intake C'(& h &W C'*% h %4 .t\CSV.dial :flush C'p  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C') +75 h .t\CTV.dial :intake C'd  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'e  +75 h #t\Flushing upper manifold C'R& h &W C'% h %4 .t\CSV.dial :flush C':  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'{ +37 h .t\CS.seek 0ml C'x'4 +507 h '4 C's& h &W C'% h %4 .t\CSV.dial :flush C'F  +37 h  .t\CS.seek 3ml C']'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'\\% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'? +76 h .t\CS.seek 0ml C'['4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +7 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&1' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'0( +416 h ( M&I& h &ӷ M&D% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &#(- +264 h (- M&j& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &^'η +91 h 'η .tHand.open M&1 +150 h 1 M&o% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [coreNEG] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST16-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:23:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST16-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1515h.pcr F*2% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*k  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *.'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +21 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*7'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*T  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *L'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*c% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*f% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1517h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ARV.dial :carrier F*r  +11 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F*y'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*n  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*O  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*m' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*P  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.536ml F**'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*p  +108 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*)'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*d n +22 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*e'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*R  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1517h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*s % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*\\'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +32 h  ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*k'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1520h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*L  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *+'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*W  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*c  +22 h ."MFBPCR"delay 2 seconds +200 F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*f n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*/ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*|% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*M % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1520h.pcr F*y% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*"  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*n'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*O'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +21 h F*_% h %n ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*m  +65 h  F*p% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1522h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*K  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Q  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*o' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*2 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*$  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*k'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Z  +108 h  ."MFBPCR"delay 7 seconds +700 F*`% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*. n +21 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*t  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*7  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*T  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*L  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CEST1539642594.97 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1522h.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*>% h %n ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +64 h  F*'% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*v  +32 h  ."MFBPCR"\ATV.dial :air3 F*4 % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +21 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*N'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1600h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +11 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*: I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*g % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*~' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.6ml F*]'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*K'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1600h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*C  +151 h F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *h' +423 h ' ."MFBPCR"\ATV.dial :ASV F*Q n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*1% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*^ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *2'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*o % +43 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :air3 F*b% h % ."MFBPCR"\AS.seek 0.53ml F*Z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +21 h F*k% h % ."MFBPCR"\AS.seek 0.71ml F*.' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h % H ."MFBPCR"\AS.seek 0.735ml F*t' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +21 h F*U% h % Ű ."MFBPCR"\AS.seek 0.72ml F*7' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*T n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*V% h % zG ."MFBPCR"\AS.seek 0.365ml F*H' +7161 h ' ."MFBPCR"\ATV.dial :waste F*L n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*3% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *;' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*+% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % ."MFBPCR"delay 3 seconds +300 F*l% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*<% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*6' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*/ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*>  +22 h  ."MFBPCR"\AS.seek 0ml F* 'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *S'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*' % +108 h % F*A% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*J% h % ."MFBPCR"\AS.seek 0.58ml F*5' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*v'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*| n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*!  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*N  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *"' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *r'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*& n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*8'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*E n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *n' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*= I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *_'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*(  +42 h  F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *P': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*w  +64 h  F*m% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b **'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*q n +65 h n F*d% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *)' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*e I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*R  +43 h  F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *G': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*g% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *s' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*~ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *F'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*x n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*\\  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*] % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*?% h % ."MFBPCR"\AS.seek 0.96ml F* '0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*K' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*D% h % ."MFBPCR"\AS.seek 0.97ml F*I'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*Q I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *1'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *^'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *j'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*2  +43 h  F*o% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*b I +43 h I ."MFBPCR"\AS.seek 0.02ml F* ' +9880 h ' ."MFBPCR"\ATV.dial :waste F*` n +129 h n ."MFBPCR"\AS.seek 0ml F*k'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*Z% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*a  +21 h ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*T% h %Z ."MFBPCR"\AS.seek 0.81ml F*V'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*H'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*}  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +64 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *c'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*>  +32 h  ."MFBPCR"\ATV.dial :air3 F*6 % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +21 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*S'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*4  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *r'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*Y % +108 h % ."MFBPCR"\AS.seek 0ml F*E'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +3562720 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' 'i h  P% %8 h  F* *= h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *n  h  .t\SPE.configure SPEconfig F*\ 2 *_% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)(% h % .t\SS.configure SSconfig C'p& S)#.0X 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:24:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #19) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&K1 +150 h 1 M&Q& h &M M&I% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &^'η +91 h 'η .tHand.close M&/ +150 h / M&2& h &- M&o% h %η .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +264 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C' & h &W .tHand.open M&k1 +150 h 1 M&a% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'B& h &W C'%% h %4 .t\CSV.dial CSR C't  +150 h  C'T% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'U'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '7% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'L'[ +463 h '[ .t\CSV.dial :waste C'V +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +2009 h '4 #t\Purging external Intake C'3& h &W C'H% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '+' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV C'  h +75 .t\CTV.dial :intake C'c  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'l'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'f& h &W C'<% h %4 .t\CSV.dial :flush C'W  +75 h  .t\CS.seek 5ml C'>'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +37 h @ C' % h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'J'4 +507 h '4 C'S& h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'5% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'v'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'|'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'z'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +7 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&"/ +150 h / .t\CC.seek :open C'( +416 h ( M&&& h &ӷ M&Y% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &0(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &-'η +91 h 'η .tHand.open M&91 +150 h 1 M&u% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:24:08CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:24:03CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*E% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K #"readyMFB"Readying the MFB C' & #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(i% h %X A(8. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (=(,9 +6 F*n% h %&K ."readyMFB"\ATV.dial :waste2 F*_ % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *(' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (p& h &,9 M&O& h &- M&w% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &m(w +166 h (w M&P& h &w A(q& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &d' +89 h ' A(*& h &,9 .t\SE.seek 10.71 Pucks below Top Plate A(e' +155 h ' A()& h &,9 M&& h &w .t\Elbow.seek 4 M&R(w +7 h (w M&:& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&@'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&g& h &w M&% h %7 .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.seek CC M&x(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +78 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)]+ +7 h + S)% h % C'D& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % .t\CBV.dial between SSV and waste2 C''  +19 h  C'/% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'A  +37 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +2610 h '4 C'% h %4 S)S% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C' @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'<9 h 9Td +100 C'c9 h 9kd +100 C'W9 h 9d +100 C'l9 h 9d +100 C' 9 h 9d +100 C'>9 h 9d +100 C''9 h 9d +100 C'/9 h 9d +100 C'A9 h 9 d +100 C'X9 h 9 d +100 C'f9 h 96d +100 C'9 h 9Ld +100 C'S9 h 9bd +100 C' 9 h 9xd +100 C'69 h 9d +100 C'J9 h 9d +100 C'49 h 9d +100 C'59 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'v9 h 9d +100 C'!9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'y9 h 9id +100 C'9 h 9d +100 C'N9 h 9d +100 C'|9 h 9d +100 C'"9 h 9d +100 C'.9 h 9d +100 C'Z9 h 9d +100 C'Y9 h 9  d +100 C'#9 h 9 d +100 C'$9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'99 h 9 j d +100 C'&9 h 9 $d +100 C'M9 h 9 (d +100 C'r9 h 9 ,d +100 C'E9 h 9 0d +100 C'9 h 9 4d +100 C'i9 h 9 8d +100 C'89 h 9 ' +23 h ' .t\SE.seek 10.04 Pucks below Top Plate A(''U +36 h 'U M&/& h & M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&f(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&6/ +150 h / .t\CC.seek :open C'J( +416 h ( A(4& h & A(5& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &!( +142 h ( M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &y' +93 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &N1 +150 h 1 M&|% h % A("& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&.'B +20 h 'B M&Z& h & A(Y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &#' +22 h ' A($% h %U ."downElevator"\SE.seek :down A('X +100 (% h % M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +62 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'M @ +19 h @ .t\CS.seek 10ml C'r' +6 h ' .t\CSV.dial between air and CTV C'E ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&81 +2 h 1 M&n& h & M&_% h %\  .t\Elbow.seek FlushPuck::Garage M&u(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M& / +150 h / M&P& h &- M&q% h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +264 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &:' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C')& h &W .tHand.open M&1 +150 h 1 M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%g% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%m' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%d  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%\ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%@  .t\CBV.dial PRV C'e  h +75 .t\PSV.dial :mixloop P%R +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%s  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial :air P%G  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%I +37 h .t\PS.seek 0ml P%]' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&(' +90 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'1( +416 h ( M&o& h &ӷ M&?% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &2(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &b'η +91 h 'η .tHand.open M&C1 +150 h 1 M&Q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'a& h & M&B% h %\  A(%% h %X A(t. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ( (,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (`& h &,9 M&~& h &- M&7% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &V(w +166 h (w M&L& h &w A(H& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(T& h &,9 .t\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' A(+& h &,9 M&& h &w .t\Elbow.seek 4 M&<(w +7 h (w M&c& h &w A(l& h &,9 .t\Forearm.seek 4 + 300 counts M& '7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &// +150 h / ."downElevator"\SE.seek :down A(A'X M&3& h &w M&U% h %7 .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.seek CC M&;(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +67 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &W' +91 h ' .t\CC.seek :closed C'z(W +416 h (W .tHand.open M&>1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'X  +151 h  .t\CSV.dial CTV C',  +19 h  .t\CS.seek 5ml C'4'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'5' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%f +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%N  +151 h  .t\PS.seek :empty P%|' +2610 h ' .t\CBV.dial :waste1 C'" @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'Z  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'Y @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'$  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'v ` +19 h ` .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'9  +38 h  .t\CS.seek 0ml C'M'4 +2647 h '4 .t\CBV.dial :waste1 C'E @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'i& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&8% h %\  A(n% h %X A(_. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (y( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (.& h & M&'& h &ӷ M&#% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &\\( +143 h ( M&p& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A(^& h & .t\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' M& & h & A(P& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &q' +26 h ' .t\Elbow.hold M&&, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(O- h -+ .t\SE.seek 10.04 Pucks below Top Plate A(0'U +27 h 'U M&% h % A(u& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&*'B +20 h 'B M&J& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 11.04 Pucks below Top Plate A(-'D +37 h 'D M&& h & M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +89 h '\  .t\Elbow.seek CC M&d(ӷ +143 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'e( +416 h ( A(R& h & A(s& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A(G& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &D' +92 h ' .t\Elbow.hold M&I, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&=% h % A(h& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&K& h & A(w& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &F' +22 h ' A([% h %D ."downElevator"\SE.seek :down A(o'X +100 (?% h % M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +52 h 'X ."downElevator"\SE.coast A(5 +38 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +113 h  P%% h % .t\PS.seek 0.499ml P%C' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%Q +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%a  +37 h .t\CBV.dial AIV C'B  +38 h .t\PRV.dial between CBV and air P%%  +19 h  .t\PRV.dial :air P%t  +19 h  P%`% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %~' +250 h ' .t\PRV.dial :waste2 P%7 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %b' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&H1 +2 h 1 M&T& h & M&2% h %\  .t\Elbow.seek FlushPuck::Garage M&+(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&l'η +91 h 'η .tHand.close M&j/ +150 h / M&/& h &- M&3% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'& h &W .tHand.open M&<1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  C'1& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%> @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%X  +75 h  .tdelay 2 seconds +200 P%,% h % .t\PS.seek 1.499ml P%W' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P% ' +1094 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.seek 0ml P%4' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%5 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%]% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%f% h %r .t\PS.seek 0.475ml P%N' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%"'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%Z  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%Y  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %$' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%v +75 h .t\PTV.dial :PRV,avoiding: :puck P%! @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%9  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%E +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%i  +75 h  .t\PS.seek 2.5ml P%8' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%n +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%_  +75 h P%.% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %'' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'# & +50 h & .t\AWV.select :waste C' +7 P%p% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'^ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %;' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%P' +2610 h ' .t\PSV.dial :PML P%O +38 h .t\PS.seek 0ml P%6' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'u& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*J @@T .t\AWV.select :air F*)  .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%g% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%k' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*\\ I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *q'6 +66 .t\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%\ ' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%0' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%&' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +22 h ' .t\PSV.dial PSR P%: +21 h  F*z% h %6 ."MFBPCR"\AS.seek 0.329ml F*R'ͯ +92 h P%s% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%G  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*d  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *=' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%h +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%K' +217 ."MFBPCR"\ATV.dial :AIV F*w I +42 h I F*[% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial PSR P%( +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%-  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*F n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*o% h % ."MFBPCR"\AS.seek 0.041ml F*y'% +336 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Q' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +2610 h ' .t\PSV.dial PSR P%B +112 h P%%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%t' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%`  +38 h  .t\PS.seek 0ml P%C' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%7 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%~' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %T'k +2679 h 'k .t\PSV.dial :waste1 P%2  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*c I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *I'V +850 h ' .t\PSV.dial :air P%j  +113 h  .t\PS.seek 2.5ml P%/' +2609 h ' .t\PSV.dial :mixloop P%3 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%l  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %x' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *U'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*e I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*<' +171 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %1' +619 h ' ."MFBPCR"\ATV.dial :waste F*> n +129 h n ."MFBPCR"\AS.seek :empty F*S'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*X% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *,' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*b  +43 h F*W% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *r'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'5% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C']' +1626 h ' ."connect"\CSV.dial CTV C'4  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*N% h %_ ."MFBPCR"\AS.seek 0.434ml F*Z'ݏ +110 h '4 .t\CSV.dial CSR C'Y  +38 h  C'"% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'v'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*!  +22 h F*$% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*9'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*E% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'i' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'n'4 +2647 h '4 C'_& h &W #tGaraging FlushPuck in Collection Clamp M&.& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +7 h (ӷ M&#& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&^/ +150 h / .t\CC.seek :open C'( +416 h ( M& & h &ӷ M&;% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &P(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &)'η +91 h 'η .tHand.open M&1 +150 h 1 M&g% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'\\& h & M&% h %\  A(m% h %X A({. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (k(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (*& h &,9 M&\ & h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &0(w +166 h (w M&@& h &w A(z& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &:' +90 h ' A(s& h &,9 ."WCR"\SE.seek 8.71 Pucks below Top Plate A(q' +175 h ' A(&& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M&R(w +7 h (w M&G& h &w A(d& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +79 h '&0 ."MFBPCR"delay 3 seconds +71 h / ."downElevator"\SE.seek :down M&& A(h'X h &w M&w% h %7 ."WCR"\Forearm.seek :retract M&['\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +54 ."MFBPCR"\ATV.dial :waste2 F*= % +4 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &(' +39 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +52 h ' ."WCR"\CC.seek :closed C'?(W +416 h (W ."WCR"Hand.open M&-1 +91 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +37 h 1 M&F% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)|% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*<' +19730 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F*X% h % ."MFBPCR"\AS.seek 0.52ml F*S' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*b n +64 h n ."MFBPCR"\ASV.dial :wasteR F*W  +22 h  ."MFBPCR"\AS.seek 0.3ml F*,': +4445 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F* 'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*5 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F*4% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b * 'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*f I +43 h I F*]% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*Y  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *"% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *r' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *!' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*$% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *Z' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*E'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*9 n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*i% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *_'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*. I +21 h I F*#% h %v ."MFBPCR"\AS.seek 0.059ml F*p'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*^ % +21 h % F*V% h %H ."MFBPCR"\AS.seek 0.069ml F*}' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.769ml F*;' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*u' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*g'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*O I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *\\' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*m'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F**% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*)% h % ."MFBPCR"\AS.seek 0.579ml F*@' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %  ."MFBPCR"\AS.seek 0.604ml F*P'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*G' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*d n +86 h n ."MFBPCR"\ASV.dial :wasteR F*6  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *w'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*0 % +108 h % F*:% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*K n +108 h n ."MFBPCR"\ASV.dial :wasteR F*=% h %  ."MFBPCR"\AS.seek 0.09ml F*q' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*R I +86 h I F*h% h % ."MFBPCR"\AS.seek 0.12ml F*[' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*F% h % ."MFBPCR"\AS.seek 0.23ml F*|' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*D'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*k% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*% n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*B  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *t'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*Q @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*y I +85 h I ."MFBPCR"\AS.seek 0ml F*H'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*7'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*2  +42 h  #"MFBPCR"\Swish #1 of 2 F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *c'\  +1032 h '\  F*L% h %\  ."MFBPCR"\AS.seek 0.5ml F*j'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*~% h %(A ."MFBPCR"\AS.seek 0.6ml F*/'\  +1031 h '\  F*l% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*3 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +3472 h 'V ."MFBPCR"\SPE.coast F*e  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1617h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*A  +11 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*+'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +21 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*,  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.53ml F*M' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*f % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*]% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +107 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*"'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*r'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Z  +43 h  ."MFBPCR"\ASV.dial :waste1 F*9  +65 h ."MFBPCR"\ARV.dial :air1 F*E  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226137 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'#  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'_ @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'V @ +76 h @ +50 S)p% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C'} ` +18 h ` ."WCR"\CS.seek 7.288ml C''' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  ';  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '8'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'O  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'g  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'{' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'\\ @ +75 h @ +50 S)m% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C')% h % ."WCR"\CSV.dial between air and CTV C'\ ` +56 h ` ."WCR"\CS.seek 7.288ml C'z' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'@  's  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '&'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'P' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'd  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'6  +38 h  C'G% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'0 @ +75 h @ +50 S):% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C'K% h % ."WCR"\CSV.dial between air and CTV C'= ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'R  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'h'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'q  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'[& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&-% h %\  A(F% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&|& h &ӷ M&(% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &k( +142 h ( M&D& h & A(%& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &B' +89 h ' A(& h & ."WCR"\SE.seek 10.71 Pucks below Top Plate A(`' +155 h ' M&a& h & A(t& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &y' +26 h ' ."WCR"\Elbow.hold M&C, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(H- h -+ M&$&.,(\ #b ."WCR"\SE.seek 11.04 Pucks below Top Plate A(2'D +27 h 'D M&T% h % A(7& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &L'B +20 h 'B M&c& h & A(~& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &j' +23 h ' ."WCR"\SE.seek 12.04 Pucks below Top Plate A(l'3 +36 h '3 M&/& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&A/ +150 h / ."WCR"\CC.seek :open C'U( +416 h ( A(I& h & A(+& h & M&& h &ӷ M&>% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &<( +142 h ( M&b& h & A(W& h & ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.seek 3 M&X' +93 h ' ."WCR"\Elbow.hold M&,, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &51 +150 h 1 M&S% h % A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&4'B +20 h 'B M& & h & A(f& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &]' +22 h ' A(M% h %3 ."downElevator"\SE.seek :down A(N'X +100 (Y% h % M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +43 h 'X ."downElevator"\SE.coast A(5 +47 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&"1 +2 h 1 M&$& h & M&!% h %\  .t\Elbow.seek FlushPuck::Garage M&r(- +166 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&E'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&v% h %η .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +264 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' .t\CC.seek :closed C'p(W +416 h (W C' & h &W .tHand.open M& 1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C';% h % .t\CSV.dial CSR C''  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'8'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'O% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'g'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'u'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'\\% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'\ % h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C's  '@  +38 h  +113  .t\CBV.dial :waste1 C'z @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'&& h &W C'd% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'P @ +38 h @ C'% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CTV C':  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'w  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #t\Flushing upper manifold C'=& h &W C'% h %4 .t\CSV.dial :flush C'R  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'q% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'[ '  +38 h +75 .t\CTV.dial :air C'-  +151 h .t\CS.seek 0ml C'F'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'|'4 +506 h '4 C'(& h &W C'D% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'a% h %0 .t\CS.seek 5ml C't'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'H'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +507 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C'7'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'c& h &W C'~% h %4 .t\CSV.dial CSR C'/  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'B'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'k' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'` +113 h .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'e  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'2  +113 h  C'j% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'C'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'A' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '?  +38 h  +113  .t\CBV.dial :waste1 C'I @ +37 h @ .t\CS.seek 0ml C'+'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'3  +75 h  .t\CS.seek 0ml C'5'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'S  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'M  +37 h +75 .t\CTV.dial :air C'<  +151 h .t\CS.seek 0ml C'Y'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing upper manifold C'$& h &W C'!% h %4 .t\CSV.dial :flush C'X  +37 h  .t\CS.seek 5ml C'4'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'Z% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'N'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C', +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'v% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'E'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'n% h %0 .t\CS.seek 5ml C'r'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing lower manifold's waste1 port C'}& h &W C'% h %4 .t\CSV.dial :flush C';  +38 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'V% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'O  .t\CTV.dial CSV C'8  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'g'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C'\\% h %4 .t\CSV.dial :flush C'm  +75 h  .t\CS.seek 5ml C'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C') @ +37 h @ C'\ % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C's  +38 h  .t\CBV.dial :waste2b C'@  +75 h  .t\CS.seek 0ml C'z'4 +766 h '4 #tDisinfecting external Intake with kill C'*& h &W C'&% h %4 .t\CSV.dial CSR C'd  +38 h  C'6% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'P' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'G  +38 h +75 .t\CTV.dial :intake C':  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'w' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'0  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '='4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'R% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'K'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'q +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +7 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.seek CC M&F' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&(& h &ӷ M&D% h % .t\Forearm.seek :retract M&%'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &|(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&t1 +150 h 1 M&H% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'o& h & .tHand.open M&c1 +2 h 1 M&~& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &/(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&`/ +150 h / M&y& h &- M&k% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C'C(W +415 h (W C'& h &W .tHand.open M&?1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'>% h %4 .t\CSV.dial CSR C'+  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '3% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'W'[ +463 h '[ .t\CSV.dial :waste C'S +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'5& h &W C' % h %4 .t\CSV.dial :flush C'M  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'< .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'f  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'Y'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C' & h &W C'$% h %4 .t\CSV.dial :flush C'!  +75 h  .t\CS.seek 5ml C'X'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'l% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV C'4 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C']'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +507 h '0 .t\CSV.dial :waste C'N +37 h .t\CS.seek 0ml C','4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'v  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'_% h %0 .t\CS.seek 5ml C'n'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'E'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'V( +416 h ( M&'& h &ӷ M&O% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\\'η +91 h 'η .tHand.open M&m1 +150 h 1 M&g% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST17-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:25:53CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST17-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1617h25ml.pcr F*\ % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*s  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*G'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*(% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1619h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*/'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*I  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*b  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*L% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*3  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*S' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*W  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*l% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1619h25ml.pcr F*]% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*9  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*U'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*m% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*J'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1621h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*=% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*#  +22 h ."MFBPCR"delay 2 seconds +200 F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *p'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*( n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*[' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*t % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +107 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*c'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*k  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*B% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1621h25ml.pcr F*/% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*e  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*?% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*M% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*4% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +21 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*X'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V @CEST1539727219.60 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1700h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*U  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*A'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*}  +22 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*8 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*\\% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*\ n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*J' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*@  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*s'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +108 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*G  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*0  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*P'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*= % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1700h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*q % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*-% h %Z ."MFBPCR"\AS.seek 0.81ml F*h'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*p'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*a  +64 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*7 % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *>'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1702h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ARV.dial :carrier F*W  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +21 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*! I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*4% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*Q % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*Z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*9  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*n  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*E  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1702h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*; @@T *A  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*C  +151 h F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *{' +423 h ' ."MFBPCR"\ATV.dial :ASV F*} n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*m  +43 h  F* % h % ."MFBPCR"\AS.seek 0.62ml F*8'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*\\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *\ 'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*i'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*g % +43 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*s% h % ."MFBPCR"\AS.seek 0.71ml F**' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h % H ."MFBPCR"\AS.seek 0.735ml F*6' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*G' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*0 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*w% h % zG ."MFBPCR"\AS.seek 0.365ml F*:' +7161 h ' ."MFBPCR"\ATV.dial :waste F*R n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*P% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*K' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*^% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*h' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*D  +22 h  ."MFBPCR"\AS.seek 0ml F*p'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *a'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*[ % +108 h % F*F% h %V ."MFBPCR"\AS.seek 0.01ml F*H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*%% h % ."MFBPCR"\AS.seek 0.58ml F*~' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*c% h % q ."MFBPCR"\AS.seek 0.605ml F*`'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +21 h F*k% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*B n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*|'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *e' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *2'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +202 h 'R ."MFBPCR"\SPE.coast F*>  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*?'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*L  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *b' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*5  +42 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *W': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*S% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*f% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *4': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*$  +64 h  F*Q% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *]'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*l n +65 h n F*9% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Z I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *X': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *v'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*, n +150 h n ."MFBPCR"\AS.seek 0ml F*n'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*_  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*.% h % ."MFBPCR"\AS.seek 0.96ml F*U'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*A% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*O% h % ."MFBPCR"\AS.seek 0.97ml F*''c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*} I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\\'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*\  +43 h  F*g% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *i' +9988 h ' ."MFBPCR"\ATV.dial AIV F*J I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*u n +129 h n ."MFBPCR"\AS.seek 0ml F*s'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*&% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*d  +21 h ."MFBPCR"\ATV.dial :air3 F** % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*0% h %Z ."MFBPCR"\AS.seek 0.81ml F*w'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*:'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*p% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +21 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*c% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast F*#,,'T(20( b ."MFBPCR"\AS.seek 0ml F*'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 1ml F*|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*> n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *2'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*I % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990367 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )L h  C' '+ h  P% %? h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ F*\ 9\ C' 2\ .t\SPE.coast P% 0\ F*\ 9\ *\ 9\ *b  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)5% h % .t\SS.configure SSconfig C'M& S)#.0X 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:26:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #20) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'O& h & .tHand.open M&1 +150 h 1 M&}& h &M M&'% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &m(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &\\'η +91 h 'η .tHand.close M&8/ +150 h / M&\ & h &- M&g% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +264 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +91 h ' .t\CC.seek :closed C'&(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'z% h %4 .t\CSV.dial CSR C'6  +150 h  C'0% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'G% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'R'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'P& h &W C':% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'=  h +75 .t\CTV.dial :intake C'#  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'-'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'K  +75 h #t\Flushing upper manifold C'(& h &W C'^% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'D'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'p% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'[ +113 h .t\CTV.dial :air .\CS.seek 0ml C'F'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'o +37 h .t\CS.seek 0ml C'%'4 +507 h '4 C'a& h &W C'% h %4 .t\CSV.dial :flush C'c  +38 h  .t\CS.seek 3ml C'H'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'~% h %0 .t\CS.seek 5ml C'k'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'`'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +7 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&e/ +150 h / .t\CC.seek :open C'>( +416 h ( M&& h &ӷ M&I% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &V'η +91 h 'η .tHand.open M& 1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:27:00CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:26:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F* % #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C';& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&L% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(+% h %X A(?. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +6 F*b% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *5' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (M& h &,9 M&3& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &S(w +166 h (w M&W& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &f' +89 h ' A( & h &,9 .t\SE.seek 7.71 Pucks below Top Plate A(' +185 h ' A(<& h &,9 M&Y& h &w .t\Elbow.seek 4 M& (w +6 h (w M&!& h &w A($& h &,9 .t\Forearm.seek 4 + 300 counts M&"'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &l/ +150 h / ."downElevator"\SE.seek :down M&Q& A(9'X h &w M&]% h %7 .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&,(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &E' +91 h ' .t\CC.seek :closed C'A(W +416 h (W .tHand.open M&1 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)n% h % C'O& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C' +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b '4' +710 h ' .t\CSV.dial CSR C'n  +150 h  C'O% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +6 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'E' +479 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %  .t\CS.seek 4.5ml C''` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .t\CS.seek 1.275ml C'}' +919 h ' #tHeating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C''   ' 9 h 9d +100 C'{9 h 9d +100 C'r9 h 91d +100 C'59 h 9J d +100 C'9 h 9bd +100 C'\ 9 h 9zd +100 C'g9 h 9d +100 C'.9 h 9d +100 C'89 h 9 d +100 C'J9 h 9$d +100 C'i9 h 9(d +100 C'm9 h 9 ,d +100 C'9 h 9$0d +100 C'U9 h 9<4d +100 C'\\9 h 9S8d +100 C'd9 h 9kd +100 C'^9 h 9Td +100 C'#9 h 9kd +100 C'9 h 9d +100 C'-9 h 9d +100 C'p9 h 9d +100 C'D9 h 9d +100 C'[9 h 9d +100 C'9 h 9d +100 C'F9 h 9 d +100 C'9 h 9 d +100 C'(9 h 96d +100 C't9 h 9Ld +100 C'a9 h 9bd +100 C'9 h 9xd +100 C'h9 h 9d +100 C'%9 h 9d +100 C'79 h 9d +100 C'~9 h 9d +100 C'c9 h 9d +100 C'H9 h 9d +100 C'`9 h 9d +100 C'y9 h 9(d +100 C'|9 h 9>d +100 C'k9 h 9Td +100 C'/9 h 9id +100 C'o9 h 9d +100 C'j9 h 9d +100 C'B9 h 9d +100 C'e9 h 9d +100 C'*9 h 9d +100 C'&9 h 9d +100 C'I9 h 9  d +100 C'9 h 9 d +100 C'@9 h 9 *d +100 C'x9 h 9 ?d +100 C')9 h 9 Td +100 C' 9 h 9 j d +100 C'9 h 9 $d +100 C'T9 h 9 (d +100 C'29 h 9 ,d +100 C' 9 h 9 0d +100 C'L9 h 9 4d +100 C'+9 h 9 8d +100 C'?9 h 9 9 h 9 d +100 C'n9 h 9 d +100 C'O9 h 9 d .t\CH.hold 5:00,85C C'4   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'E'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 5ml C' 'p +1373 h 'p .t\CSV.dial CTV C'{  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'r @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'5  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'\ '4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'g +113 h .t\CBV.dial CSV C'.  +19 h C'8% h %4 .t\CS.seek 5ml C'J'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'i  +38 h C'm% h %p .t\CS.seek 7ml C''i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'U @ +37 h @ .t\CS.seek 10ml C'\\' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'd ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&z% h %\  A(6% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (s( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (u& h & M&Z& h &ӷ M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&w& h & A(R& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &P' +90 h ' A(:& h & .t\SE.seek 11.71 Pucks below Top Plate A(q'Ɛ +145 h 'Ɛ M&0& h & A(=& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&K, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+Ɛ .t\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M&^% h % A(#& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&-& h & A(p& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &D' +23 h ' .t\SE.seek 13.04 Pucks below Top Plate A(['" +36 h '" M&& h & M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&((ӷ +143 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'%( +416 h ( A(7& h & A(~& h & M&c& h &ӷ M&H% h % .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &y( +142 h ( M&|& h & A(k& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 3 M&/' +93 h ' .t\Elbow.hold M&o, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &j1 +150 h 1 M&B% h % A(e& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&*'B +20 h 'B M&&& h & A(I& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(@% h %" ."downElevator"\SE.seek :down A(x'X +100 ()% h % M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +34 h 'X ."downElevator"\SE.coast A(5 +56 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'T @ +19 h @ .t\CS.seek 10ml C'2' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'L { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&?1 +2 h 1 M&b& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&1(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &- .t\Forearm.seek :garage M&3'η +91 h 'η .tHand.close M&;/ +150 h / M&W& h &- M&% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &!' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'<& h &W .tHand.open M&l1 +150 h 1 M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%$ +151 h P%Q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%S' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%f  +38 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'"  P%  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%>' +2610 h ' .t\PSV.dial :mixloop P%n +38 h .t\PS.seek 0ml P%O' +2609 h ' .t\PSV.dial :air P%4  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%} +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +7 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.seek CC M&5' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&g& h &ӷ M&.% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &\ (- +265 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &J'η +91 h 'η .tHand.open M&U1 +150 h 1 M&'% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'd& h & M&% h %\  A(z% h %X A(6. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (u& h &,9 M&Z& h &- M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &w(w +166 h (w M&R& h &w A(:& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(0& h &,9 .t\SE.seek 6.71 Pucks below Top Plate A(=' +196 h ' A(& h &,9 M&^& h &w .t\Elbow.seek 4 M&#(w +6 h (w M&X& h &w A(-& h &,9 .t\Forearm.seek 4 + 300 counts M&p'7 +23 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&P& A(F'X h &w M&% h %7 .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +104 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&D1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'A  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'~' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'c'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%H  +37 h  .t\PS.seek 2.5ml P%|' +2610 h ' .t\PSV.dial :mixloop P%k +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%( +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%j  +151 h  .t\PS.seek :empty P%B' +2609 h ' .t\CBV.dial :waste1 C'e @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'&  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'I @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'@  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'` ` +19 h ` .t\CSV.dial :air C'y @ +37 h @ .t\CS.seek 10ml C')' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'T'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'L  +151 h C'+& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&?% h %\  A(b% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (/( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (*& h & M&[& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &_( +143 h ( M&M& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &s' +89 h ' A(\\& h & .t\SE.seek 12.71 Pucks below Top Plate A(o' +136 h ' M&;& h & A(W& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(3- M&$&.,(\ #b h -+ .t\SE.seek 13.04 Pucks below Top Plate A('" +27 h '" M&Y% h % A(1& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M& 'B +20 h 'B M&%& h & A(<& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &l' +22 h ' .t\SE.seek 14.04 Pucks below Top Plate A(V' +37 h ' M&$& h & M&Q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +89 h '\  .t\Elbow.seek CC M&f(ӷ +143 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&]' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'"( +416 h ( A( & h & A(& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &n( +143 h ( M&>& h & A(4& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &O' +92 h ' .t\Elbow.hold M&}, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A({& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&r'B +21 h 'B M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &v' +22 h ' A(% h % ."downElevator"\SE.seek :down A(g'X +100 (.% h %v M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +22 h 'X ."downElevator"\SE.coast A(5 +68 h '\  .t\SC.coast .\PSV.dial :air A(6 P%m  +113 h  P%E% h % .t\PS.seek 0.499ml P%U' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%' +38 h .t\PRV.dial :air P%8  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%d  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%z  +19 h  .t\PRV.dial :air P%6  +19 h  P%u% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %Z' +250 h ' .t\PRV.dial :waste2 P%G @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %J' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&:1 +2 h 1 M&0& h & M&\ % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&-'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&P% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +264 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &a' +91 h ' .t\CC.seek :closed C'p(W +416 h (W C'=& h &W .tHand.open M +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  C' & h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%D @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%K  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%A% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%2  +38 h  .t\PS.seek 0ml P%7' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%~ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%H  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %k'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%(% h %r .t\PS.seek 0.475ml P%j' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%|  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%e'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%&  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%I  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %@' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%` +75 h .t\PTV.dial :PRV,avoiding: :puck P%y @ +76 h @ .t\PS.seek 0ml P%c' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%)' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%L' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%+  +75 h  .t\PS.seek 2.5ml P%?' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%b +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%*% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %[' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%M% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %x'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'\\ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :PML P%3 +38 h .t\PS.seek 0ml P%Y' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '1& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'h& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*% @@T *<  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%$ +21 h  ."MFBPCR"delay 20 seconds +92 h P%Q% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%s' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*_ I +129 h I F*o% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%S  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%l +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%! +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%4  +37 h  .t\PS.seek 0ml P%]' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*f  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%{ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F* I +42 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*9' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial PSR P%5 +113 h P%O% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%r' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%V  +38 h  .t\PS.seek 0ml P%m' +739 h ' ."MFBPCR"delay 5 seconds +500 F*E9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*v n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g% h % ."MFBPCR"\AS.seek 0.041ml F*/'% +336 h ' .t\PSV.dial PSR P%B +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%'' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%8 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial PSR P% +112 h P%z% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%U' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%G +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%Z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%: +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %0'k +2679 h 'k .t\PSV.dial :waste1 P%\  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %^' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*X I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *}'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%P +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %i' +5106 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %,' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*t  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*" I +43 h I F*=% h %Q ."MFBPCR"\AS.seek 0.01ml F*#' +171 h ' .t\PSV.dial :mixloop P%F +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb % ' +619 h ' ."MFBPCR"\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.seek :empty F*a'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*K  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *A' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*J  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *2'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'H'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' .t\CTV.dial CSV ."connect"\CSV.dial CTV C'7  '  +37 h  +113  .t\CBV.dial :waste1 C'( @ +38 h @ .t\CS.seek 0ml C'k'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*|  +22 h ."MFBPCR"delay 3 seconds +300 F*j% h %_ ."MFBPCR"\AS.seek 0.434ml F*&'ݏ +110 h '4 .t\CSV.dial CSR C'I  +38 h  C'e% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'`'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*y  +22 h F*@% h %ݏ ."MFBPCR"\AS.seek 0.419ml F* '& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*c n +108 h n F* % h %& ."MFBPCR"\AS.seek 0.092ml F*)'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'+' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'L  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'b'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.seek CC M&[' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C'x( +416 h ( M&;& h &ӷ M&% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +264 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &<'η +91 h 'η .tHand.open M&$1 +150 h 1 M&Q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'_& h & M&o% h %\  A(S% h %X A(N. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (s(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ( & h &,9 M&& h &- M&l% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &!' +90 h ' A(& h &,9 ."WCR"\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' A(& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M& (w +6 h (w M&4& h &w A(f& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +50 h '&0 ."MFBPCR"delay 3 seconds +100 h / ."downElevator"\SE.seek :down A(>'X M&{& h &w M&% h %7 ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&](ӷ +109 ."MFBPCR"\ATV.dial :waste2 F* % +5 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&n& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +9 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *O'R +82 h ' ."WCR"\CC.seek :closed C'.(W +416 h (W ."WCR"Hand.open M&V1 +61 h 'R ."MFBPCR"\ATV.dial :air2 F*r  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *E' +67 h 1 M&v% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)9+ +6 h + S)B% h % C'm& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'8  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*) % +21 h % F*x% h % ."MFBPCR"\AS.seek 0.13ml F*r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*v% h % ."MFBPCR"\AS.seek 0.23ml F*B' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h % ."MFBPCR"\AS.seek 0.255ml F*8'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*s% h %X ."MFBPCR"\AS.seek 0.24ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*z n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *6'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*' @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*/ I +85 h I ."MFBPCR"\AS.seek 0ml F*:'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\  +42 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *X'\  +1032 h '\  F*R% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*Z% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*-% h %\  ."MFBPCR"\AS.seek 0.5ml F*i'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*t% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *^' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*P % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +3472 h 'V ."MFBPCR"\SPE.coast F*"  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1717h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*=  +64 h  ."MFBPCR"\ARV.dial :carrier F*F  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*#  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*J  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *a'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*A  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*~% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*H  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*T' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*7'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +107 h  ."MFBPCR"delay 7 seconds +700 F*I% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*| n +22 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*@  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*c' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*L% h % \\ ."MFBPCR"\AS.seek 0.326ml F*+'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226166 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'w @ +75 h @ +50 S)M% h % ."WCR"\CBV.dial between CSV and waste2b C'p  +94 h  C';% h % ."WCR"\CSV.dial between air and CTV C'q ` +19 h ` ."WCR"\CS.seek 7.288ml C'[' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'1  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '?'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'$  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'h' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'3  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Q  +38 h  C'o% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'N' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'_ @ +76 h @ +50 S)S% h % ."WCR"\CBV.dial between CSV and waste2b C'l  +94 h  C'<% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'4  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +37 h  C'Y% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)!% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'n' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '>'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ')' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'x  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&V% h %\  A(v% h %X A(r. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (m( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (O& h & M&B& h &ӷ M&5% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &s( +143 h ( M&8& h & A(z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(9& h & ."WCR"\SE.seek 13.71 Pucks below Top Plate A(u'n +125 h 'n M&d& h & A(6& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &/' +26 h ' ."WCR"\Elbow.hold M&U, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(:- h -+n M&$&.,(\ #b ."WCR"\SE.seek 14.04 Pucks below Top Plate A(\ ' +27 h ' M&0% h % A(G& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&R'B +20 h 'B M&X& h & A(Z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +23 h ' ."WCR"\SE.seek 15.04 Pucks below Top Plate A(-' +36 h ' M&& h & M&t% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +89 h '\  ."WCR"\Elbow.seek CC M&P(ӷ +143 h (ӷ M&^& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ ."WCR"\Forearm.seek CC M&=' +91 h ' ."WCR"Hand.close M&F/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(}& h & A(& h & M&E& h &ӷ M&D% h % ."WCR"\Forearm.seek :retract M&K'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &#( +142 h ( M&J& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&A, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &~1 +150 h 1 M&a% h % A(H& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&& h & A((& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(j'X +100 (I% h %- M&7% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +13 h 'X ."downElevator"\SE.coast A(5 +77 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'k& h & .tHand.open M&e1 +2 h 1 M&@& h & M&y% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +166 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&L/ +150 h / M&c& h &- M&`% h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'p& h &W .tHand.open M&;1 +150 h 1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'1% h % .t\CSV.dial CSR C'[  +38 h  C'$% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'?'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '3% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Q'[ +463 h '[ .t\CSV.dial :waste C'o +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'h'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'N& h &W C'_% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'l'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'4% h %4 .t\CSV.dial :flush C'f  +75 h  .t\CS.seek 5ml C'Y'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'!  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'& h &W C'{% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +37 h @ C'% h %p .t\CS.seek 7ml C'>'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'x '  +38 h +75 .t\CTV.dial :air C'V  +151 h .t\CS.seek 0ml C'v'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +506 h '0 .t\CSV.dial :waste C'O +38 h .t\CS.seek 0ml C'B'4 +506 h '4 C'5& h &W C'8% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.seek 3ml C'9'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'd% h %0 .t\CS.seek 5ml C'6'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C':'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +507 h '0 .t\CSV.dial :waste C'G +75 h .t\CS.seek 0ml C'X'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'Z& h &W C'g% h %4 .t\CSV.dial CSR C'  +150 h  C't% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C's' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u +113 h .t\CTV.dial :intake C'/  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'^' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'"  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'\  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  '.  +38 h  +113  .t\CBV.dial :waste1 C'} @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'E  +38 h  C'D% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  +38 h  .t\CBV.dial :waste2b C'P  +75 h  .t\CS.seek 0ml C'~'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'a  +37 h  C'H% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'K 'T  +37 h +75 .t\CTV.dial :air C'#  +151 h .t\CS.seek 0ml C'I'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'k +38 h .t\CS.seek 0ml C'e'4 +507 h '4 #t\Flushing upper manifold C'@& h &W C'y% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'&% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'j'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'm'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'L'4 +507 h '4 C'c& h &W C'`% h %4 .t\CSV.dial :flush C'|  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %0 .t\CS.seek 5ml C'2'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'*'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'p +76 h .t\CS.seek 0ml C';'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'q& h &W C'% h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.seek 5ml C'['p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'w% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CTV C'3  .t\CTV.dial CSV C'?  +37 h  +113  .t\CBV.dial :waste1 C'o @ +38 h @ .t\CS.seek 0ml C'Q'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'N& h &W C'_% h %4 .t\CSV.dial :flush C'S  +75 h  .t\CS.seek 5ml C'h'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'% h %p .t\CS.seek 7ml C'l'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C' & h &W C'% h %4 .t\CSV.dial CSR C'4  +38 h  C'f% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'W' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'Y  +38 h +75 .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'{  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +7 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&O/ +150 h / .t\CC.seek :open C'r( +416 h ( M&5& h &ӷ M&8% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &9'η +91 h 'η .tHand.open M&61 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&Z1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&u/ +150 h / M&/& h &- M&s% h %η .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +265 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &R' +91 h ' .t\CC.seek :closed C'U(W +415 h (W C'i& h &W .tHand.open M&.1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'D% h %4 .t\CSV.dial CSR C'  +113 h  C'J% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'='p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'P% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'a +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +2009 h '4 #t\Purging external Intake C'~& h &W C'% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# +75 h .t\CTV.dial :intake C'(  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'I'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'k  +75 h #t\Flushing upper manifold C'7& h &W C'@% h %4 .t\CSV.dial :flush C'y  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +37 h @ C'-% h %p .t\CS.seek 7ml C'&'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'j +37 h .t\CS.seek 0ml C'A'4 +507 h '4 C'm& h &W C'c% h %4 .t\CSV.dial :flush C'`  +37 h  .t\CS.seek 3ml C'|'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'% h %0 .t\CS.seek 5ml C'b'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'2'0 +506 h '0 .t\CSV.dial :waste C'* +76 h .t\CS.seek 0ml C'p'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'w( +416 h ( M&[& h &ӷ M&3% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &o(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &_'η +91 h 'η .tHand.open M&S1 +150 h 1 M&Q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST18-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:28:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST18-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1717h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*f'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*{% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*>  +32 h  ."MFBPCR"\ATV.dial :air3 F*O % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*5% h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*d'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*:'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1719h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*r n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*}  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*J  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*P  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*a' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +108 h  ."MFBPCR"delay 7 seconds +700 F*H% h %< ."MFBPCR"\AS.seek 0.594ml F*#'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*( n +22 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*I  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*e  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*-% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*& % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1719h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *`'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*L% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +65 h  F*$% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *^'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*N  +32 h  ."MFBPCR"\ATV.dial :air3 F*q % +86 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*_% h % ."MFBPCR"\AS.seek 0.81ml F*%'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*l% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1721h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *M'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*5 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*\\% h %Fj ."MFBPCR"\AS.seek 0.524ml F*v' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*] I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*x' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*6 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*:  +107 h  ."MFBPCR"delay 7 seconds +700 F*0% h %< ."MFBPCR"\AS.seek 0.594ml F*Z'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*g n +22 h n ."MFBPCR"delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.6ml F*X'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*s  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*G % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1721h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*"  +22 h ."MFBPCR"\ATV.dial :air3 F*i % +87 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F*J'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*P'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*~  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*a  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +31 h  ."MFBPCR"\ATV.dial :air3 F*I % +87 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*m% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V @CEST1539813619.60 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1800h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*^% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*N  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*? I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*_% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*%' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*4'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1800h25ml.pcr F*{% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*n  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*>'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*M'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*6  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +64 h  F*x% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *:'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*u  +32 h  ."MFBPCR"\ATV.dial :air3 F*X % +86 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*i% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*r  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1802h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*K n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*I  +21 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *@'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*y I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*-' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*' % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*&' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*m% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +22 h  ."MFBPCR"delay 7 seconds +700 F*c% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*L n +21 h n ."MFBPCR"delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.6ml F*|'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*b  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F**' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*^ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1802h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*1 @@T *F  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*U  +151 h F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *N' +423 h ' ."MFBPCR"\ATV.dial :ASV F*q n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*S  +43 h  F*;% h % ."MFBPCR"\AS.seek 0.62ml F*?'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*_ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*+'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *l' +424 h ' ."MFBPCR"\ATV.dial :air3 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h % H ."MFBPCR"\AS.seek 0.735ml F*f' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*Y' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*!' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*W% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*V% h %  ."MFBPCR"\AS.seek 0.604ml F*O'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +21 h F*\\% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*>' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*] n +86 h n ."MFBPCR"\ASV.dial :wasteR F*8  +22 h  ."MFBPCR"\AS.seek 0ml F*M'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *d'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*x % +108 h % F*v% h %V ."MFBPCR"\AS.seek 0.01ml F*:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*z% h % ."MFBPCR"\AS.seek 0.58ml F*g' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h % q ."MFBPCR"\AS.seek 0.605ml F*u'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +21 h F*s% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*/  +21 h  ."MFBPCR"\AS.seek 0ml F*B'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *"' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*i n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *\ '^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*r n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +202 h 'R ."MFBPCR"\SPE.coast F*D  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*.'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*E n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*R  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *J' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*= I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*~  +42 h  F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*H  +64 h  F*a% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*K n +65 h n F*(% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *#' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*k I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*7  +43 h  F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*@  +64 h  F*'% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*- n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *m' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*& I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*c  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*L n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *`'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*A n +150 h n ."MFBPCR"\AS.seek 0ml F*b'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*| % +43 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F**% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %0 ."MFBPCR"\AS.seek 0.985ml F*$' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*3% h % ."MFBPCR"\AS.seek 0.97ml F*['c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*q I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *;'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *)'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *+' +9988 h ' ."MFBPCR"\ATV.dial AIV F*% I +43 h I ."MFBPCR"\AS.seek 0.02ml F*l' +9880 h ' ."MFBPCR"\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*4  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*!'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*W% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*8  +32 h  ."MFBPCR"\ATV.dial :air3 F*> % +86 h % F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*Z% h %m ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*X  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *\ '(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*} % +108 h % ."MFBPCR"\AS.seek 0ml F*E'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990367 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )R h  C' ' h  P% %. h  F* *= h  C' 9\ P% 9\ % 2\ C' 0\ S)&\ P% *\ F*\ 9\ .t\SPE.coast C' -\ P% 0\ F*\ 9\ C' 2\ F*\ 9\ *J  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)~% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'T& S)#.0X 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:30:00CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #21) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'3& h & .tHand.open M&$1 +150 h 1 M&q& h &M M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &S(- +166 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &_'η +91 h 'η .tHand.close M&?/ +150 h / M&& h &- M&Q% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'l& h &W .tHand.open M&1 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'f  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'Y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '{'4 +2009 h '4 #t\Purging external Intake C'W& h &W C'!% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'n .t\CBV.dial CSV C'  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'V'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'5& h &W C'\\% h %4 .t\CSV.dial :flush C'O  +75 h  .t\CS.seek 5ml C'8'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +37 h @ C'M% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'x +113 h .t\CTV.dial :air .\CS.seek 0ml C'v'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'6'0 +507 h '0 .t\CSV.dial :waste C'0 +37 h .t\CS.seek 0ml C'z'4 +507 h '4 C'd& h &W C't% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.seek 3ml C':'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'g% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'u'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'/ +75 h .t\CS.seek 0ml C'9'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&B& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&"/ +150 h / .t\CC.seek :open C'D( +416 h ( M&r& h &ӷ M&}% h % .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &o(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &w'η +91 h 'η .tHand.open M&C1 +150 h 1 M&,% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:30:06CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:30:00CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*<% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C'1& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&R% h %\  A(% h %X A(.. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (=(,9 +6 F*J% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *~' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (T& h &,9 M&P& h &- M&H% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &a(w +166 h (w M&& h &w A(K& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &(' +89 h ' A(& h &,9 .t\SE.seek 4.71 Pucks below Top Plate A(k' +214 h ' A(#& h &,9 M&I& h &w .t\Elbow.seek 4 M&7(w +7 h (w M&y& h &w A('& h &,9 .t\Forearm.seek 4 + 300 counts M&e'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &-/ +150 h / ."downElevator"\SE.seek :down A( 'X M&@& h &w M&% h %7 .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.seek CC M&A(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +20 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +90 h ' .t\CC.seek :closed C'^(W +416 h (W .tHand.open M&F1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)|+ +7 h + S)b% h % C'3& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)M  +144 h  +6  S)8% h % .t\CBV.dial between SSV and waste2 C'x  +19 h  C']% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'v  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +2609 h '4 C'6% h %4 S)d% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C't @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'\\9 h 9Td +100 C'n9 h 9kd +100 C'O9 h 9d +100 C'V9 h 9d +100 C'M9 h 9d +100 C'89 h 9d +100 C'x9 h 9d +100 C']9 h 9d +100 C'v9 h 9 d +100 C'{9 h 9 d +100 C'59 h 96d +100 C'69 h 9Ld +100 C'd9 h 9bd +100 C't9 h 9xd +100 C'>9 h 9d +100 C'z9 h 9d +100 C'X9 h 9d +100 C'g9 h 9d +100 C'Z9 h 9d +100 C':9 h 9d +100 C'u9 h 9d +100 C'/9 h 9(d +100 C'B9 h 9>d +100 C's9 h 9Td +100 C'9 h 9id +100 C'09 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'}9 h 9  d +100 C' 9 h 9 d +100 C'9 h 9 *d +100 C'i9 h 9 ?d +100 C'E9 h 9 Td +100 C'C9 h 9 j d +100 C'r9 h 9 $d +100 C'G9 h 9 (d +100 C'\ 9 h 9 ,d +100 C'<9 h 9 0d +100 C'R9 h 9 4d +100 C'9 h 9 8d +100 C'.9 h 9 / +150 h / .t\CC.seek :open C'z( +416 h ( A(X& h & A(g& h & M&Z& h &ӷ M&:% h % .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &/( +142 h ( M&B& h & A(s& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&0, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A("& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 & 'B +20 h 'B M&& h & A(}& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(i'X +100 (E% h %X M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'G @ +19 h @ .t\CS.seek 10ml C'\ ' +6 h ' .t\CSV.dial between air and CTV C'< ` +19 h ` .t\AIV.dial :bypass C'R { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&.1 +2 h 1 M&J& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&,(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.seek :garage M&P'η +91 h 'η .tHand.close M&1/ +150 h / M&& h &- M&K% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +264 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C''(W +416 h (W C'p& h &W .tHand.open M&-1 +150 h 1 M&w% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%y +151 h P%@% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%c' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%e  C'k  h +75 .t\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%m  h .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%F  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P%b +38 h .t\PS.seek 0ml P%3' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%=' +2610 h ' .t\PSV.dial :mixloop P%[ +37 h .t\PS.seek 0ml P%|' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&L& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.seek CC M&~' +90 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C';( +416 h ( M&Q& h &ӷ M&*% h % .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &%'η +91 h 'η .tHand.open M&U1 +150 h 1 M&q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'4& h & M&% h %\  A(% h %X A(f. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (l(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (h& h &,9 M&&& h &- M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M& & h &w A(!& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &A' +89 h ' A(& h &,9 .t\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' A(& h &,9 M&\\& h &w .t\Elbow.seek 4 M&n(w +7 h (w M&V& h &w A(M& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &]/ +150 h / ."downElevator"\SE.seek :down A(v'X M&W& h &w M&% h %7 .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +132 h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'9(W +416 h (W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'{  +151 h  .t\CSV.dial CTV C'^  +18 h  .t\CS.seek 5ml C'X'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'g' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%:  +38 h  .t\PS.seek 2.5ml P%B' +2609 h ' .t\PSV.dial :mixloop P%s +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%t  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%5 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C'" @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'} @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'u ` +19 h ` .t\CSV.dial :air C'/ @ +38 h @ .t\CS.seek 10ml C'E' +2647 h ' .t\CSV.dial CTV C'C  +37 h  .t\CS.seek 0ml C'G'4 +2648 h '4 .t\CBV.dial :waste1 C'< @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'R  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&.% h %\  A(J% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&x& h &ӷ M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&T& h & A(i& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(_& h & .t\SE.seek 15.71 Pucks below Top Plate A(0'L +107 h 'L M&1& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &K' +25 h ' .t\Elbow.hold M&r, +7 h , .t\Elbow.reconfigure TightElbow A(P- M&$&.,(\ #b h -+L .t\SE.seek 16.04 Pucks below Top Plate A(o' +26 h ' M&#% h % A(,& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'B +21 h 'B M&z& h & A(p& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &-' +22 h ' .t\SE.seek 17.04 Pucks below Top Plate A(w' +36 h ' M&y& h & M&@% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  .t\Elbow.seek CC M&((ӷ +143 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&e/ +150 h / .t\CC.seek :open C'k( +416 h ( A(7& h & A(m& h & M&c& h &ӷ M&F% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &b( +142 h ( M&D& h & A(& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 3 M&3' +93 h ' .t\Elbow.hold M&[, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &=1 +150 h 1 M&L% h % A(N& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&2'B +21 h 'B M&$& h & A(H& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &`' +22 h ' A('% h % ."downElevator"\SE.seek :down A(Q'X +92 h 'X ."downElevator"\SE.coast A(5 +8 (*% h %X M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +89 h '\  .t\SC.coast .\PSV.dial :air A(6 P%S  +113 h  P% % h % .t\PS.seek 0.499ml P%U' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%q +37 h .t\PRV.dial :air P%?  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%4  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%f  +19 h  P%h% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %&' +250 h ' .t\PRV.dial :waste2 P%Y @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %%' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&!1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&)(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&M/ +150 h / M&]& h &- M&W% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &d' +91 h ' .t\CC.seek :closed C'(W +415 h (W C'& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C';& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%8 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%{  +75 h  .tdelay 2 seconds +200 P%^% h % .t\PS.seek 1.499ml P%O' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%l' +1095 h ' .t\PSV.dial :air P%\  +37 h  .t\PS.seek 0ml P%X' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%g +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%:  +38 h  .tdelay 2 seconds +200 P%|% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %s'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%t  +75 h .tdelay 2 seconds +200 P%5% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%B  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%"'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%}  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%u +76 h .t\PTV.dial :PRV,avoiding: :puck P%/ @ +75 h @ .t\PS.seek 0ml P%Z' +2610 h ' .t\PSV.dial :air P%C  +75 h  .t\PS.seek 2.5ml P%E' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%< +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%R' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%.' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%J +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %x' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%T% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %i'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'_ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%1  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P%P +38 h .t\PS.seek 0ml P%#' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 ',& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'z& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*p @@T *>  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%y +22 h  ."MFBPCR"delay 20 seconds +91 h P%@% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*0% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *K'6 +66 .t\PSV.dial :waste1 P%a  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%- +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%r' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*e  +22 h ' .t\PSV.dial PSR P%I +21 h  F*9% h %6 ."MFBPCR"\AS.seek 0.329ml F*7'ͯ +92 h P%m% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%c' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*F % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*(  +22 h  F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *L' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%$' +217 ."MFBPCR"\ATV.dial :AIV F*H I +43 h I F*'% h % ."MFBPCR"\AS.seek 0.469ml F* ' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%b +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%*' +2609 h ' .t\PSV.dial PSR P%~ +113 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +37 h  .t\PS.seek 0ml P%S' +740 h ' ."MFBPCR"delay 5 seconds +500 F* 9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*Q n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*`% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%q' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%4' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%f' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%h  +38 h  .t\PS.seek 0ml P%U' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%Y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%&' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%! +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*\\ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *['V +849 h ' .t\PSV.dial :air P%M  +113 h  .t\PS.seek 2.5ml P%]' +2610 h ' .t\PSV.dial :mixloop P%W +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %+' +5107 h ' .t\PSV.dial :air P%V  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %A' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*6  +42 h  F*)% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*k I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*n' +171 h ' .t\PSV.dial :mixloop P%v +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %;' +618 h ' ."MFBPCR"\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.seek :empty F*d'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*{% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *^' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*%  +43 h F*O% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *\ '_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'l  +38 h  C'g% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C':'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'|' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  't  +38 h  +113  .t\CBV.dial :waste1 C'5 @ +37 h @ .t\CS.seek 0ml C's'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*B  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +109 h '4 .t\CSV.dial CSR C'}  +38 h  C'"% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'u'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*/  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*C'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Z n +108 h n F*<% h %& ."MFBPCR"\AS.seek 0.092ml F*E'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'R  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'J'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +6 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.seek CC M&x' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'i( +416 h ( M&1& h &ӷ M&% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &>'η +91 h 'η .tHand.open M&y1 +150 h 1 M&@% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'& h & M&0% h %\  A(a% h %X A(j. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&& h &- M&-% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &o(w +166 h (w M&e& h &w A(9& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &I' +89 h ' A(m& h &,9 ."WCR"\SE.seek 2.71 Pucks below Top Plate A(K') +235 h ') A(r& h &,9 M&F& h &w ."WCR"\Elbow.seek 4 M&7(w +6 h (w M&& h &w A((& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&='7 +23 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +19 h '&0 ."MFBPCR"delay 3 seconds +131 h / ."downElevator"\SE.seek :down M&D& A(N'X h &w M&H% h %7 ."WCR"\Forearm.seek :retract M&''\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +79 ."MFBPCR"\ATV.dial :waste2 F*L % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +21 h (ӷ M&$& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' + h 'X ."downElevator"\SE.coast A(5 +90 h ' ."WCR"\CC.seek :closed C'*(W +416 h (W ."WCR"Hand.open M&w1 +32 h 'R ."MFBPCR"\ATV.dial :air2 F*2  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b * ' +96 h 1 M&Q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +6 h + S)% h % C'S& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'?  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.seek 0.579ml F*e' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*r% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*F' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*(  +43 h  ."MFBPCR"\AS.seek 0ml F*c'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *H'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*o % +108 h % F*I% h %V ."MFBPCR"\AS.seek 0.59ml F*L' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +107 h n ."MFBPCR"\ASV.dial :wasteR F*~% h %  ."MFBPCR"\AS.seek 0.09ml F*K' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*7 I +86 h I F*E% h % ."MFBPCR"\AS.seek 0.12ml F*'' +625 h ' ."MFBPCR"\ATV.dial :air3 F*N % +22 h % F*i% h % ."MFBPCR"\AS.seek 0.13ml F*2' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F* ' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *f'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*q @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*!'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *\\'\  +1031 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*M'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*&% h %(A ."MFBPCR"\AS.seek 0.6ml F*]'\  +1032 h '\  F*V% h %\  ."MFBPCR"\AS.seek 0.5ml F*+'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*6% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*W % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +3472 h 'V ."MFBPCR"\SPE.coast F*k  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1817h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +65 h  ."MFBPCR"\ARV.dial :carrier F*v  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*n  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*%  +22 h ."MFBPCR"delay 2 seconds +200 F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*^  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*:  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*G' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*5 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.536ml F*t'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*"'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*B n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.65ml F*Z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*C  +64 h ."MFBPCR"\ARV.dial :air1 F*<  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*R% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226195 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '_' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)T% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'1% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'x' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C',  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '.'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'y  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'z' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'P  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'@  +37 h  C'0% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'j' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)a% h % ."WCR"\CBV.dial between CSV and waste2b C'-  +94 h  C'>% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'9' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'm  'e  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '#'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'r  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'(  +37 h  C'F% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'c' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'o @ +75 h @ +50 S)I% h % ."WCR"\CBV.dial between CSV and waste2b C'H  +94 h  C'$% h % ."WCR"\CSV.dial between air and CTV C'~ ` +57 h ` ."WCR"\CS.seek 7.288ml C'L' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'=  '7  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'E'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'K  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'N' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'i  +150 h C''& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&w% h %\  A(Q% h %X A(2. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (S( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (b& h & M&& h &ӷ M&3% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&?& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h & ."WCR"\SE.seek 16.71 Pucks below Top Plate A(h'; +96 h '; M&4& h & A(f& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M&U, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(!- h -+; M&$&.,(\ #b ."WCR"\SE.seek 17.04 Pucks below Top Plate A(' +27 h ' M&% h % A(Y& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M& 'B +21 h 'B M&\\& h & A(&& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &M' +22 h ' ."WCR"\SE.seek 18.04 Pucks below Top Plate A(V' +36 h ' M&]& h & M&6% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +90 h '\  ."WCR"\Elbow.seek CC M&W(ӷ +142 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&v/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A([& h & A()& h & M& & h &ӷ M&8% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &n( +142 h ( M&%& h & A(O& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &{' +93 h ' ."WCR"\Elbow.hold M&^, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &g1 +150 h 1 M&d% h % A(:& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&X'B +21 h 'B M&l& h & A(5& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &|' +22 h ' A(G% h % ."downElevator"\SE.seek :down A('X +83 h 'X ."downElevator"\SE.coast A(5 +17 (}% h %X M&t% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +89 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C's& h & .tHand.open M&"1 +2 h 1 M&& h & M&/% h %\  .t\Elbow.seek FlushPuck::Garage M&\ (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&R/ +150 h / M&Z& h &- M&u% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'& h &W .tHand.open M&11 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C',% h % .t\CSV.dial CSR C'x  +38 h  C'y% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'.'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'P% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '@'[ +463 h '[ .t\CSV.dial :waste C'0 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'z'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'j& h &W C'% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '-'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'e  'm  +38 h  +113  .t\CBV.dial :waste1 C'9 @ +75 h @ .t\CS.seek 0ml C'r'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'#& h &W C'% h %4 .t\CSV.dial :flush C'(  +76 h  .t\CS.seek 5ml C'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'c% h %p .t\CS.seek 7ml C'o'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'I  +38 h  .t\CBV.dial :waste2b C'H  +75 h  .t\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing upper manifold C'~& h &W C'D% h %4 .t\CSV.dial :flush C'7  +76 h  .t\CS.seek 5ml C'='p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'K% h %p .t\CS.seek 7ml C'E'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'i ''  +37 h +75 .t\CTV.dial :air C'w  +151 h .t\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'2'0 +507 h '0 .t\CSV.dial :waste C'b +37 h .t\CS.seek 0ml C''4 +507 h '4 C'3& h &W C'?% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'f'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'!'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'Y +76 h .t\CS.seek 0ml C'\\'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'&& h &W C'`% h %4 .t\CSV.dial CSR C']  +151 h  C'6% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'k  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'M% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'U'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'+  '*  +38 h  +113  .t\CBV.dial :waste1 C'[ @ +38 h @ .t\CS.seek 0ml C')'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'8% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'O' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'W  +75 h  .t\CS.seek 0ml C'g'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'd  +38 h  C':% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'l'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'G  +38 h +75 .t\CTV.dial :air C'n  +151 h .t\CS.seek 0ml C'}'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C's +37 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing upper manifold C'& h &W C'/% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.seek 5ml C'X'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C'|'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +507 h '0 .t\CSV.dial :waste C'^ +38 h .t\CS.seek 0ml C'R'4 +506 h '4 C'Z& h &W C'u% h %4 .t\CSV.dial :flush C'B  +38 h  .t\CS.seek 3ml C'<'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'J% h %0 .t\CS.seek 5ml C'\ 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'1'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C',  +37 h  .t\CS.seek 5ml C'x'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +37 h @ C'% h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'P  '.  +38 h  +113  .t\CBV.dial :waste1 C'0 @ +37 h @ .t\CS.seek 0ml C'@'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'j& h &W C'% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CTV C'e  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'm  +76 h  .t\CS.seek 0ml C'9'4 +765 h '4 #tDisinfecting external Intake with kill C'& h &W C'#% h %4 .t\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'r'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c 'F  +37 h +76 .t\CTV.dial :intake C'I  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'H' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'o  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +606 h '4 .t\CSV.dial CSR C'D  +113 h  C'7% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'$'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'L' +1372 h ' .t\CSV.dial CBV C'K +113 h .t\CS.seek 0ml C'='4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&'& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +6 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.seek CC M&Q' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C'2( +416 h ( M&3& h &ӷ M&?% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&f1 +150 h 1 M&!% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&&1 +2 h 1 M&`& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &](- +7 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&h/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'U(W +416 h (W C'+& h &W .tHand.open M&*1 +150 h 1 M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'8% h %4 .t\CSV.dial CSR C')  +113 h  C'%% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'O'[ +462 h '[ .t\CSV.dial :waste C'd +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ':'4 +2010 h '4 #t\Purging external Intake C'g& h &W C'l% h %4 .t\CSV.dial :flush C'G  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'n +75 h .t\CTV.dial :intake C'5  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'}'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C's  +75 h #t\Flushing upper manifold C't& h &W C'% h %4 .t\CSV.dial :flush C'/  +76 h  .t\CS.seek 5ml C'{'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C'V% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'| +38 h .t\CS.seek 0ml C'^'4 +506 h '4 C'S& h &W C'Z% h %4 .t\CSV.dial :flush C'u  +38 h  .t\CS.seek 3ml C'B'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'% h %0 .t\CS.seek 5ml C'J'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'<'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.seek CC M&1' +91 h ' .tHand.close M&y/ +150 h / .t\CC.seek :open C'( +416 h ( M&x& h &ӷ M&.% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &0(- +264 h (- M&N& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&a1 +150 h 1 M&@% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST19-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:32:14CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST19-Oct-18" +263690 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1817h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*e  +21 h ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*('> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*F'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*o  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*D% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *7'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +32 h  ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*f% h %m ."MFBPCR"\AS.seek 0.82ml F*!'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1819h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*M% h %V ."MFBPCR"\AS.seek 0.5ml F*]'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *+'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*%  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*=' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*W  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*d' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*O  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*l'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*G  +107 h  ."MFBPCR"delay 7 seconds +700 F*:% h %< ."MFBPCR"\AS.seek 0.594ml F*n'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*s'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*t  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1819h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*C  +22 h ."MFBPCR"\ATV.dial :air3 F*S % +86 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*R% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\ % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +64 h  F*y% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*j  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*a% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*p'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*-% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*#  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1821h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*7  +11 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*b  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*3 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*_% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*i' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*f % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*!  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*` n +21 h n ."MFBPCR"delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.6ml F*\\'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1821h25ml.pcr F*]% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*k  +22 h ."MFBPCR"\ATV.dial :air3 F*+ % +86 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*%'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*=% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +64 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *:'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +21 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*S% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*|  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V @CEST1539900019.58 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1900h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*v'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*x n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*j  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *N'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*P I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*p' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*m  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*#  +108 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.6ml F*r'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*I' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*F  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*o  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1900h25ml.pcr F*D% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*L  +22 h ."MFBPCR"\ATV.dial :air3 F*K % +87 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *b'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*E'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +21 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*f  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +65 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *!'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +31 h  ."MFBPCR"\ATV.dial :air3 F*\\ % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*M'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +21 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*2  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1902h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*G  +42 h  ."MFBPCR"\ARV.dial :carrier F*O  +11 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*l'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*}  +22 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*X% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*q % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +22 h  ."MFBPCR"delay 7 seconds +700 F*Z% h %< ."MFBPCR"\AS.seek 0.594ml F*^'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*<  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*y'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1902h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*, @@T *v  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*U  +150 h F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *j' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*a  +43 h  F*1% h % ."MFBPCR"\AS.seek 0.62ml F*P'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*@ % +43 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *-' +424 h ' ."MFBPCR"\ATV.dial :air3 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*e% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*(' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*r% h % Ű ."MFBPCR"\AS.seek 0.72ml F*F' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*o n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*H% h % zG ."MFBPCR"\AS.seek 0.365ml F*I' +7162 h ' ."MFBPCR"\ATV.dial :waste F*7 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *~'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*D % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *L' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +21 h F*'% h % ."MFBPCR"\AS.seek 0.579ml F*$' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h %  ."MFBPCR"\AS.seek 0.604ml F*b'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*_% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*E' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*?  +22 h  ."MFBPCR"\AS.seek 0ml F*T'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *4'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*i % +107 h % F*Q% h %V ."MFBPCR"\AS.seek 0.01ml F*!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*`' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h % q ."MFBPCR"\AS.seek 0.605ml F*h'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *]'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*M  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *k' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*+ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*2 n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +202 h 'R ."MFBPCR"\SPE.coast F*8  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F**'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *%' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*g  +43 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *O': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*:  +65 h  F*d% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *l'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*5% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *n' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*t  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *X': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*q% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*V n +65 h n F*C% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *S' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*Z  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *{': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*R n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *u'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*^ n +151 h n ."MFBPCR"\AS.seek 0ml F*J'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*B % +43 h % F*\ % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h %0 ."MFBPCR"\AS.seek 0.985ml F*y' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +21 h F*.% h % ."MFBPCR"\AS.seek 0.97ml F*x'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *1'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *N'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*p I +43 h I ."MFBPCR"\AS.seek 0.02ml F*-' +9880 h ' ."MFBPCR"\ATV.dial :waste F*z n +128 h n ."MFBPCR"\AS.seek 0ml F*e'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*#% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *r'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*o% h %Z ."MFBPCR"\AS.seek 0.81ml F*H'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +21 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*I'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +65 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +31 h  ."MFBPCR"\ATV.dial :air3 F*E % +87 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*!'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +21 h F*&% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *k'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*[ % +107 h % ."MFBPCR"\AS.seek 0ml F* 'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990369 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )  h  C' ') h  P% %* h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *%  h  .t\SPE.configure SPEconfig F*\ 2 *=% h %V .t\AS.configure ASconfig S)g% h % .t\SS.configure SSconfig F*#,,'T(^20( b C'G& h & .t\CC.configure CCconfig S)#.0X 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:33:15CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #22) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'.& h & .tHand.open M&y1 +150 h 1 M&& h &M M&x% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &a(- +166 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&P/ +150 h / M&& h &- M&@% h %η .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +264 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +91 h ' .t\CC.seek :closed C'#(W +416 h (W C'-& h &W .tHand.open M&e1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'c& h &W C'9% h %4 .t\CSV.dial CSR C'(  +150 h  C'o% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'r'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'F% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '7'[ +463 h '[ .t\CSV.dial :waste C'H +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2009 h '4 #t\Purging external Intake C'& h &W C'I% h %4 .t\CSV.dial :flush C'K  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'~ 'L  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'w'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'$  +75 h #t\Flushing upper manifold C'3& h &W C'_% h %4 .t\CSV.dial :flush C'b  +75 h  .t\CS.seek 5ml C'?'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +37 h @ C'T% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'i +113 h .t\CTV.dial :air .\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'4& h &W C'6% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.seek 3ml C'!'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\\ @ +38 h @ C'`% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'h'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'8( +416 h ( M&2& h &ӷ M&[% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &A'η +91 h 'η .tHand.open M& 1 +150 h 1 M&;% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 17:33:20CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:33:15CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*>% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C',& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M& % h %\  A()% h %X A(*. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +6 F*%% h %&K ."readyMFB"\ATV.dial :waste2 F*= % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *g' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (G& h &,9 M&W& h &- M&:% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &d(w +166 h (w M&O& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &5' +89 h ' A(l& h &,9 .t\SE.seek 1.71 Pucks below Top Plate A(s': +244 h ': A(n& h &,9 M&}& h &w .t\Elbow.seek 4 M&t(w +7 h (w M&/& h &w A(q& h &,9 .t\Forearm.seek 4 + 300 counts M&"'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down A(C'X M&& h &w M&% h %7 .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.seek CC M&^(ӷ +142 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&v1 +150 h 1 M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)B+ +7 h + S)J% h % C'.& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  .t\SS.seek 0ml S)#.0Xd +100 C'_9 h 9Td +100 C' 9 h 9kd +100 C'b9 h 9d +100 C'w9 h 9d +100 C'T9 h 9d +100 C'?9 h 9d +100 C'i9 h 9d +100 C'9 h 9d +100 C'Q9 h 9 d +100 C'D9 h 9 d +100 C'39 h 96d +100 C'f9 h 9Ld +100 C'49 h 9bd +100 C'69 h 9xd +100 C'E9 h 9d +100 C'9 h 9d +100 C'\\9 h 9d +100 C'`9 h 9d +100 C'&9 h 9d +100 C'!9 h 9d +100 C'h9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C']9 h 9id +100 C'9 h 9d +100 C'M9 h 9d +100 C'9 h 9d +100 C'k9 h 9d +100 C'9 h 9d +100 C'#9 h 9d +100 C'[9 h 9  d +100 C'09 h 9 d +100 C'm9 h 9 *d +100 C'+9 h 9 ?d +100 C'9 h 9 Td +100 C' 9 h 9 j d +100 C'29 h 9 $d +100 C'Y9 h 9 (d +100 C'9 h 9 ,d +100 C'>9 h 9 0d +100 C' 9 h 9 4d +100 C')9 h 9 8d +100 C'*9 h 9  ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C')& h & .tHand.open M&*1 +2 h 1 M&%& h & M&=% h %\  .t\Elbow.seek FlushPuck::Garage M&;(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &- .t\Forearm.seek :garage M&W'η +91 h 'η .tHand.close M&,/ +150 h / M&O& h &- M&% h %η .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C'q(W +416 h (W C'& h &W .tHand.open M&V1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%/ +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%d' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Z' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C's  P%"  h +75 .t\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%C  h .t\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial :air P%v  +37 h  .t\PS.seek 2.5ml P%8' +2610 h ' .t\PSV.dial :mixloop P%J +38 h .t\PS.seek 0ml P%.' +2609 h ' .t\PSV.dial :air P%X  +38 h  .t\PS.seek 2.5ml P%R' +2610 h ' .t\PSV.dial :mixloop P%x +37 h .t\PS.seek 0ml P%B' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +7 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&g' +90 h ' .tHand.close M&y/ +150 h / .t\CC.seek :open C'1( +416 h ( M&@& h &ӷ M& % h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &p'η +91 h 'η .tHand.open M&U1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'& h & M&c% h %\  A(9% h %X A((. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (-(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (z& h &,9 M&& h &- M&F% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &H(w +166 h (w M&7& h &w A(I& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &^' +89 h ' A(o& h &,9 .t\SE.seek 0.71 Pucks below Top Plate A(L'K +254 h 'K A('& h &,9 M&_& h &w .t\Elbow.seek 4 M& (w +6 h (w M&w& h &w A(T& h &,9 .t\Forearm.seek 4 + 300 counts M&{'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(Q'X h &w M&r% h %7 .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.seek CC M&~(ӷ +143 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &b' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&?1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'D  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'\\'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'`' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'&'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%!  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%6  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%3 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%M  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'k @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'#  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'[ @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'm  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'h ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'Y'4 +2647 h '4 .t\CBV.dial :waste1 C'> @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C')& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&*% h %\  A(%% h %X A(=. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (]( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&i& h &ӷ M&+% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &0( +142 h ( M&& h & A(G& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &e' +90 h ' A(& h & .t\SE.seek 18.71 Pucks below Top Plate A(' +77 h ' M&,& h & A(O& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&2, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(W- h -+ .t\SE.seek 19.04 Pucks below Top Plate A(' +26 h ' M&n% h % A(;& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&l'B +21 h 'B M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &V' +22 h ' .t\SE.seek 20.04 Pucks below Top Plate A(A' +36 h ' M&/& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&"/ +150 h / .t\CC.seek :open C's( +416 h ( A(t& h & A(C& h & M&Z& h &ӷ M&v% h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &J( +142 h ( M&8& h & A(X& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 3 M&.' +93 h ' .t\Elbow.hold M&x, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&% h % A(j& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&\ 'B +21 h 'B M&y& h & A(:& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &u' +22 h ' A(q% h % ."downElevator"\SE.seek :down A(@'X +63 h 'X ."downElevator"\SE.coast A(5 +37 ( % h %X M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\SC.coast .\PSV.dial :air A(6 P%a  +113 h  P%<% h % .t\PS.seek 0.499ml P%U' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%P  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'c  +37 h .t\PRV.dial between CBV and air P%9  +19 h  .t\PRV.dial :air P%(  +19 h  P%z% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%F @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %p' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&I1 +2 h 1 M&o& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&w'η +91 h 'η .tHand.close M&T/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +265 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &4' +91 h ' .t\CC.seek :closed C'L(W +415 h (W C'{& h &W .tHand.open M& 1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  C'1& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%? @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%$  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%D  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%b' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%-' +1095 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%\\' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%` +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%!  +38 h  .tdelay 2 seconds +200 P%B% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%6  +75 h .tdelay 2 seconds +200 P%3% h %r .t\PS.seek 0.475ml P%M' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%k'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%#  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%[  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %m' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%h +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%&' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%> +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%)  +75 h  .t\PS.seek 2.5ml P%*' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%=  +76 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %i' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'+ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %G'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %~' +1313 h ' .t\PSV.dial :air P%,  +38 h  .t\PS.seek 2.5ml P%O' +2609 h ' .t\PSV.dial :PML P%W +38 h .t\PS.seek 0ml P%n' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 ';& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C F* @@T C'& ."MFBPCR"\ASV.dial :SPEdelivery F*E  h &W .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%/ +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%e' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*0 I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%d  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%l +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%V +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%2' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*"  +22 h ' .t\PSV.dial PSR P%} +21 h  F* % h %6 ."MFBPCR"\AS.seek 0.329ml F*t'ͯ +92 h P%C% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Z' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*v % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%X  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%j +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +217 ."MFBPCR"\ATV.dial :AIV F*: I +43 h I F*q% h % ."MFBPCR"\AS.seek 0.469ml F*S' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P%g +113 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%\ ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +37 h  .t\PS.seek 0ml P%a' +740 h ' ."MFBPCR"delay 5 seconds +500 F*<9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*@ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*u% h % ."MFBPCR"\AS.seek 0.041ml F*]'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%c +113 h P%9% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%(' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%z  +38 h  .t\PS.seek 0ml P%U' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%F +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%I +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %o'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %'' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*_ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *x'V +849 h ' .t\PSV.dial :air P%T  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%w  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %^' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*f  +42 h  F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *r'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*s I +43 h I F*{% h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +171 h ' .t\PSV.dial :mixloop P%K +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %1' +618 h ' ."MFBPCR"\ATV.dial :waste F*? n +129 h n ."MFBPCR"\AS.seek :empty F*4'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*$  +86 h  F*D% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*p  +43 h F*b% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'-  +38 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'B' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\\  '6  +38 h  +113  .t\CBV.dial :waste1 C'3 @ +37 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*M% h %_ ."MFBPCR"\AS.seek 0.434ml F*#'ݏ +109 h '4 .t\CSV.dial CSR C'[  +38 h  C'k% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'h'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*m% h %ݏ ."MFBPCR"\AS.seek 0.419ml F* '& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*& n +108 h n F*>% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C')' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'%'4 +2647 h '4 C'=& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +6 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'G( +416 h ( M&,& h &ӷ M&~% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +265 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &E'η +91 h 'η .tHand.open M&/1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'0& h & M&% h %\  A(d% h %X A(|. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (e(,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (l& h &,9 M&& h &- M&V% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&"& h &w A( & h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &}' +89 h ' A(C& h &,9 ."WCR"\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ A(2& h &,9 M&v& h &w ."WCR"\Elbow.seek 4 M&t(w +7 h (w M&X& h &w A(5& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&R'7 +12 h '&0 ."MFBPCR"delay 3 seconds +10 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Z/ +150 h / ."downElevator"\SE.seek :down M&j& A(8'X h &w M&:% h %7 ."WCR"\Forearm.seek :retract M&q'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +49 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +51 h (ӷ M&y& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' ."WCR"\CC.seek :closed C' (W +416 h (W ."WCR"Hand.open M&A1 +2 h 'R ."MFBPCR"\ATV.dial :air2 F*\  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *<' +126 h 1 M&@% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +90 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)S+ +7 h + S)% h % C'a& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'P  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *='v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F*+% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*H% h %H ."MFBPCR"\AS.seek 0.069ml F*Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*,% h % ."MFBPCR"\AS.seek 0.769ml F*~' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +21 h F*/% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*W I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *0' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek :empty F*d'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*| % +43 h % F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +21 h F*E% h % ."MFBPCR"\AS.seek 0.579ml F*"' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %  ."MFBPCR"\AS.seek 0.604ml F*O'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*2% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*v' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*X n +85 h n ."MFBPCR"\ASV.dial :wasteR F*5  +43 h  ."MFBPCR"\AS.seek 0ml F*Z'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *:'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*}% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*y n +107 h n ."MFBPCR"\ASV.dial :wasteR F*g% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*t I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*q' +625 h ' ."MFBPCR"\ATV.dial :air3 F*8 % +22 h % F*G% h % ."MFBPCR"\AS.seek 0.13ml F*\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*@% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +21 h % ."MFBPCR"delay 3 seconds +300 F*J% h % ."MFBPCR"\AS.seek 0.255ml F*P'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*e% h %X ."MFBPCR"\AS.seek 0.24ml F*S' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*9 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*c  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *('5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*] I +86 h I ."MFBPCR"\AS.seek 0ml F*I'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*F'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 F*#,,'T20 b ."MFBPCR"\AS.seek 0.6ml F*_'\  +1031 h '\  F*7% h %\  ."MFBPCR"\AS.seek 0.5ml F*T'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*w% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*f% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *'' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +3472 h 'V ."MFBPCR"\SPE.coast F*s  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1917h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*{  +65 h  ."MFBPCR"\ARV.dial :carrier F*K  +10 h  F*r% h %V ."MFBPCR"\AS.seek 0.5ml F*N'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +22 h ."MFBPCR"delay 2 seconds +200 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*-' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*!  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*3 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +107 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*m  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*>  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226225 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'+  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'= @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'H @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'L  +94 h  C',% h % ."WCR"\CSV.dial between air and CTV C'Q ` +19 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C';  '~  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '*'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'/  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'|' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'0 @ +75 h @ +50 S)d% h % ."WCR"\CBV.dial between CSV and waste2b C'V  +94 h  C'E% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C' ' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'"  'C  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'n'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'2  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'O' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'X  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'5  +37 h  C'v% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'Z' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)}% h % ."WCR"\CBV.dial between CSV and waste2b C':  +94 h  C'y% h % ."WCR"\CSV.dial between air and CTV C'g ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'R  't  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '8' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'G  +151 h C'q& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&A% h %\  A(@% h %X A(\ . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (a( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (J& h & M&& h &ӷ M&.% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &e( +143 h ( M&P& h & A(9& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &c' +89 h ' A(S& h & ."WCR"\SE.seek 19.71 Pucks below Top Plate A(z' +66 h ' M&& h & A((& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &]' +25 h ' ."WCR"\Elbow.hold M&U, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(I- h -+ ."WCR"\SE.seek 20.04 Pucks below Top Plate A(' +27 h ' M&o% h % A(F& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&7'B +21 h 'B M&_& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &T' +22 h ' ."WCR"\SE.seek 21.04 Pucks below Top Plate A(w' +36 h ' M&& h & M&f% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&'& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ ."WCR"\Forearm.seek CC M&{' +91 h ' ."WCR"Hand.close M&K/ +150 h / ."WCR"\CC.seek :open C'r( +416 h ( A(x& h & A(N& h & M&<& h &ӷ M&?% h % ."WCR"\Forearm.seek :retract M&$'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +142 h ( M&p& h & A(b& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &D' +93 h ' ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &`1 +150 h 1 M&4% h % A(!& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&\\'B +21 h 'B M&-& h & A(3& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &B' +22 h ' A(Y% h % ."downElevator"\SE.seek :down A(M'X +53 h 'X ."downElevator"\SE.coast A(5 +47 ([% h %X M&6% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&k1 +2 h 1 M&m& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&>'η +91 h 'η .tHand.close M& / +150 h / M&&& h &- M&h% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'L& h &W .tHand.open M&,1 +150 h 1 M&Q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C';& h &W C'~% h % .t\CSV.dial CSR C'i  +38 h  C'/% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'*'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'|& h &W C'0% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C'% h %p .t\CS.seek 7ml C'l'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  '"  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'2'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'n& h &W C'X% h %4 .t\CSV.dial :flush C'5  +76 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +38 h @ C'Z% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'}  +38 h  .t\CBV.dial :waste2b C':  +75 h  .t\CS.seek 0ml C'y'4 +766 h '4 #t\Flushing upper manifold C'g& h &W C'j% h %4 .t\CSV.dial :flush C't  +76 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G 'q  +37 h +75 .t\CTV.dial :air C'A  +151 h .t\CS.seek 0ml C'@'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C'J +37 h .t\CS.seek 0ml C''4 +507 h '4 C'.& h &W C'P% h %4 .t\CSV.dial :flush C'9  +37 h  .t\CS.seek 3ml C'S'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'(% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'I'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +506 h '0 .t\CSV.dial :waste C'F +76 h .t\CS.seek 0ml C'_'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'% h %4 .t\CSV.dial CSR C'f  +151 h  C'u% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'c'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'e' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z +113 h .t\CTV.dial :intake C']  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C''' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C's  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '7'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'T% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'U'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'x @ +38 h @ .t\CS.seek 0ml C'N'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'<  +38 h  C'?% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'p'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'b' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'`'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'4  +38 h  C'!% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '-'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Y '$  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'['4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'6'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'k'4 +507 h '4 #t\Flushing upper manifold C'm& h &W C'% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'M% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C'B'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'&& h &W C'h% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'>'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'%% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C','4 +507 h '4 #t\Flushing lower manifold's waste1 port C'Q& h &W C';% h %4 .t\CSV.dial :flush C'~  +37 h  .t\CS.seek 5ml C'i'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'H% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  '*  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'|& h &W C'0% h %4 .t\CSV.dial :flush C'd  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C'% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  +37 h  .t\CBV.dial :waste2b C'"  +76 h  .t\CS.seek 0ml C' '4 +765 h '4 #tDisinfecting external Intake with kill C'l& h &W C'n% h %4 .t\CSV.dial CSR C'X  +38 h  C'5% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'2'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'O' +416 h ' ."connect"\CSV.dial CBV C'Z .t\CBV.dial CSV C'v  +37 h +76 .t\CTV.dial :intake C'}  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C':' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'g'4 +606 h '4 .t\CSV.dial CSR C'j  +113 h  C't% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'R'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +6 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&J/ +150 h / .t\CC.seek :open C'\ ( +416 h ( M&.& h &ӷ M&P% h % .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &S'η +91 h 'η .tHand.open M&1 +150 h 1 M&I% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'o& h & .tHand.open M&1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &f(- +7 h (- M&u& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&z/ +150 h / M&]& h &- M&e% h %η .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' .t\CC.seek :closed C'U(W +416 h (W C'& h &W .tHand.open M& 1 +150 h 1 M&x% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'<& h &W C'?% h %4 .t\CSV.dial CSR C'N  +113 h  C'p% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'{'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'b'[ +462 h '[ .t\CSV.dial :waste C'4 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '!'4 +2010 h '4 #t\Purging external Intake C'`& h &W C'-% h %4 .t\CSV.dial :flush C'$  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Y' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'3  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'['4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'6& h &W C'm% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'w% h %p .t\CS.seek 7ml C'M'i +416 h 'i ."connect"\CSV.dial CBV C'\\ .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'#'0 +507 h '0 .t\CSV.dial :waste C'B +38 h .t\CS.seek 0ml C''4 +506 h '4 C'a& h &W C'&% h %4 .t\CSV.dial :flush C'h  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'=% h %0 .t\CS.seek 5ml C'%'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'>'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'L'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&r& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +6 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.seek CC M&,' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'H( +416 h ( M&i& h &ӷ M&W% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M&d1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST20-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:35:06CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST20-Oct-18" +263690 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1917h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*C  +21 h ."MFBPCR"\ATV.dial :air3 F*" % +87 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *l'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*X% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*g' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*J % +86 h % F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*('m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1919h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F*f'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*x  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +22 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *N'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*p  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*4' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*b  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.536ml F*-'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*$  +107 h  ."MFBPCR"delay 7 seconds +700 F*!% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*M % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1919h25ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*#  +22 h ."MFBPCR"\ATV.dial :air3 F*a % +86 h % F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *h'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*>'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*~  +64 h  F*/% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*d% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +21 h F*0% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*C'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*n  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1921h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*O  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*g% h %V ."MFBPCR"\AS.seek 0.5ml F*y'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*J  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*+  +21 h ."MFBPCR"delay 2 seconds +200 F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*. n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.53ml F*G' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.536ml F*9'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +107 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*S  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*]' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*e  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*T  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1921h25ml.pcr F*f% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*s  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*p'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*R% h %n ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*4  +64 h  F*$% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *!'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *w' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*M'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V @CEST1539986419.58 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *~'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2000h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*r  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*K'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*|  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Q  +21 h ."MFBPCR"delay 2 seconds +200 F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F** I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*"  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F*C'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*n  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*X'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*l n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*5  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*v  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*O'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2000h25ml.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *J'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*(  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *I'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*z  +31 h  ."MFBPCR"\ATV.dial :air3 F*_ % +87 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*T'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *?'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2002h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*$  +42 h  ."MFBPCR"\ARV.dial :carrier F*b  +11 h  F*!% h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*[  +22 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*\\% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*#  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*B  +22 h  ."MFBPCR"delay 7 seconds +700 F*&% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*%  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*>  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*;% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2002h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*~ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*K  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*U  +150 h F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *|' +424 h ' ."MFBPCR"\ATV.dial :ASV F*Q n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*d  +43 h  F*,% h % ."MFBPCR"\AS.seek 0.62ml F**'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*0 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*)'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *V' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*C% h % ."MFBPCR"\AS.seek 0.71ml F*l' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h % H ."MFBPCR"\AS.seek 0.735ml F*5' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*2% h % Ű ."MFBPCR"\AS.seek 0.72ml F*v' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*:% h % zG ."MFBPCR"\AS.seek 0.365ml F*}' +7162 h ' ."MFBPCR"\ATV.dial :waste F*t n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *g'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*j % +43 h % F*O% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*q% h % ."MFBPCR"\AS.seek 0.579ml F*y' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*A% h %  ."MFBPCR"\AS.seek 0.604ml F*J'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*P  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *('V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*G % +107 h % F*@% h %V ."MFBPCR"\AS.seek 0.01ml F*I' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +21 h F*9% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*z'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*e% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*S' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*c n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*]  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *f'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*T  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *s' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*\ n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +202 h 'R ."MFBPCR"\SPE.coast F*?  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*< n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*7  +43 h  F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *p' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*{ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*`  +43 h  F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *b': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*!  +65 h  F*4% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *-'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Y n +64 h n F*3% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*6  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\\': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*m  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*w n +65 h n F*#% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *a' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*M I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*&  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *D': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *h'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*%'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*=  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*r'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %0 ."MFBPCR"\AS.seek 0.985ml F*/' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +21 h F*W% h % ."MFBPCR"\AS.seek 0.97ml F*i'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*Q I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *,'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *0'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *8'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *)' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*V' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*C'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*n% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*l % +43 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +21 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*P  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +21 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*_  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*? n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*x % +107 h % ."MFBPCR"\AS.seek 0ml F*<'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990369 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )7 h  C' 'N h  P% %  h  F* *{ h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *p  h  .t\SPE.configure SPEconfig F*\ 2 *R% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)`% h % .t\SS.configure SSconfig C'$& S)#.0X% h %\  P%'& C'K& h &2 ."openPC"\PC.reconfigure PCconfig h &3 .t\CC.reconfigure CCconfig P%$x2 ^22b ."openPC"\PC.home.seek :open .t\CC.home.seek :open P%r( C'$x2 ^22b 'U( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 14:00:15 began in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:36:06CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #23) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&/1 +150 h 1 M&Q& h &M M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &d(- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &0'η +91 h 'η .tHand.close M&*/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'n(W +416 h (W C'V& h &W .tHand.open M&C1 +150 h 1 M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Z& h &W C' % h %4 .t\CSV.dial CSR C'5  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '2'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +463 h '[ .t\CSV.dial :waste C': +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +2009 h '4 #t\Purging external Intake C'O& h &W C'}% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'g  h +75 .t\CTV.dial :intake C'+  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'A'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'y  +75 h #t\Flushing upper manifold C'.& h &W C'% h %4 .t\CSV.dial :flush C'J  +75 h  .t\CS.seek 5ml C'P'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G +113 h .t\CTV.dial :air .\CS.seek 0ml C'@'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'o +37 h .t\CS.seek 0ml C'9'4 +507 h '4 C'(& h &W C'u% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'I'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +507 h '0 .t\CSV.dial :waste C'] +75 h .t\CS.seek 0ml C'S'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +7 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&s/ +150 h / .t\CC.seek :open C'?( +416 h ( M&\ & h &ӷ M&x% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &^'η +91 h 'η .tHand.open M&<1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 17:36:11CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:36:06CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*E% #tLoading sh1 puck from tube 5 into Collection Clamp h %&K C'~& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&7% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(N% h %X A( . h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ({(; +6 F*p% h %&K ."readyMFB"\ATV.dial :waste2 F*R % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *`' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b ($& h &; M&& h &- M&!% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &4(M +166 h (M M&b& h &M A(Y& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &3'x +90 h 'x A(-& h &; .t\SE.seek 20.71 Pucks below Top Plate A(' +56 h ' A( & h &; M&[& h &M .t\Elbow.seek 5 M&6(M +7 h (M M&& h &M A(& h &; .t\Forearm.seek 5 + 300 counts M&k' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &w/ +150 h / ."downElevator"\SE.seek :down M&m& A(#'X h &M M& % h % .t\Forearm.seek :retract M&a'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +90 h ' .t\CC.seek :closed C''(W +416 h (W .tHand.open M&K1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)%% h % C'W& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +479 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %  .t\CS.seek 4.5ml C'{'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'  +37 h  .t\CS.seek 1.275ml C'i' +919 h ' #tHeating sh1 puck from tube 5 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'Q   ',9 h 9d +100 C'|9 h 9d +100 C'9 h 91d +100 C'`9 h 9J d +100 C'/9 h 9bd +100 C'9 h 9zd +100 C'9 h 9d +100 C'9 h 9d +100 C'*9 h 9 d +100 C'9 h 9$d +100 C')9 h 9(d +100 C'd9 h 9 ,d +100 C'89 h 9$0d +100 C'U9 h 9<4d +100 C'09 h 9S8d +100 C'X9 h 9kd +100 C'9 h 9Td +100 C'+9 h 9kd +100 C'J9 h 9d +100 C'A9 h 9d +100 C'9 h 9d +100 C'P9 h 9d +100 C'G9 h 9d +100 C'9 h 9d +100 C'@9 h 9 d +100 C'j9 h 9 d +100 C'.9 h 96d +100 C'9 h 9Ld +100 C'(9 h 9bd +100 C'u9 h 9xd +100 C'9 h 9d +100 C'99 h 9d +100 C'_9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'I9 h 9d +100 C'z9 h 9d +100 C']9 h 9(d +100 C'9 h 9>d +100 C'e9 h 9Td +100 C'f9 h 9id +100 C'o9 h 9d +100 C'T9 h 9d +100 C'c9 h 9d +100 C's9 h 9d +100 C'l9 h 9d +100 C'n9 h 9d +100 C'x9 h 9  d +100 C'9 h 9 d +100 C'"9 h 9 *d +100 C'9 h 9 ?d +100 C'L9 h 9 Td +100 C'<9 h 9 j d +100 C'\ 9 h 9 $d +100 C'F9 h 9 (d +100 C'9 h 9 ,d +100 C'E9 h 9 0d +100 C'79 h 9 4d +100 C'N9 h 9 8d +100 C' 9 h 9 '4 +423 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +112 h @ .t\CSV.dial :air C'{ @ +38 h @ .t\CS.seek 5ml C','p +1372 h 'p .t\CSV.dial CTV C'|  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'`  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'/ @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'  +19 h C'*% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C')  +38 h C'd% h %p .t\CS.seek 7ml C'8'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'U @ +38 h @ .t\CS.seek 10ml C'0' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'X ` +131 h ` #tLysing Complete C'Z& h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M& % h %\  A(5% h %X A(V. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (C(,9 +201 h (,9 .t\SC.reconfigure SCloose M&M& A($.(\ 1~b (& h &ӷ .t\Elbow.reconfigure TightElbow h &,9 .t\Elbow.seek CC ."raiseStack"\SE.seek :upperLimit M&$&.,(\ #b &v(ӷ A(2'Z +6 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&O' +91 h ' .tHand.close M&}/ +150 h / .t\CC.seek :open C'( +23 h 'Z +393 ( A(& h &,9 A(g& h &,9 M&& h &ӷ M&y% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &S(w +143 h (w M&& h &w A(+& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &J' +92 h '  .t\Elbow.hold M&A, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&P% h %  A(G& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&'7 +21 h '7 M&@& h &w A(j& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &.' +22 h ' A(% h %Z ."downElevator"\SE.seek :down A(('X +100 (u% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'_ @ + A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'I { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'z& h & .tHand.open M&]1 +2 h 1 M&& h &w M&e% h %\  .t\Elbow.seek FlushPuck::Garage M&f(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&s/ +150 h / M&l& h &- M&n% h %η .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C'<(W +416 h (W C'\ & h &W .tHand.open M&F1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%7 +150 h P%N% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%p  +38 h  .t\PS.seek 0ml P%R' +1597 h ' .t\PSV.dial PSR P%1 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +2609 h ' ."connect"\PRV.dial CBV P%$  .t\CBV.dial PRV C'  h +76 .t\PSV.dial :mixloop P%~ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%b  h .t\PS.seek 0ml P%Y' +2609 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 2.5ml P%!' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%[' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%H' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%m' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&^& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&4' +91 h ' .tHand.close M&3/ +150 h / .t\CC.seek :open C'h( +416 h ( M&;& h &ӷ M&&% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&K1 +150 h 1 M&?% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'W& h & M&\\% h %\  A( % h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +201 h (; .t\SC.reconfigure SCloose A($.(\ 1~b ({& h &; M&>& h &- M&|% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &(M +166 h (M M&`& h &M A(/& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &,'x +89 h 'x A(& h &; .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(*& h &; M&& h &M .t\Elbow.seek 5 M&)(M +6 h (M M&d& h &M A(& h &; .t\Forearm.seek 5 + 300 counts M&U' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Q/ +150 h / ."downElevator"\SE.seek :down M&X& A(8'X h &M M&Z% h % .t\Forearm.seek :retract M& '\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&5(ӷ +142 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C':(W +416 h (W .tHand.open M&t1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'D  +150 h  .t\CSV.dial CTV C''  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'g' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%y  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%+ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%O  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%v +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%P' +2609 h ' .t\CBV.dial :waste1 C'G @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'@  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'j @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'q ` +19 h ` .t\CSV.dial :air C'S @ +37 h @ .t\CS.seek 10ml C'u' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'J'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'_  +151 h C'2& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&% h %\  A(I% h %X A(z. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (](,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&e& h &ӷ M&.% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &o(w +143 h (w M&T& h &w A((& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &C' +89 h ' A(0& h &,9 .t\SE.seek -0.29 Pucks below Top Plate A(A'\\ +264 h '\\ M&s& h &w A(l& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &n' +26 h '  .t\Elbow.hold M&9, +6 h ,w .t\Elbow.reconfigure TightElbow A(c- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A("' +27 h ' M&% h %  A(f& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&x'7 +20 h '7 M&& h &w A(\ & h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &F' +23 h ' .t\SE.seek 1.04 Pucks below Top Plate A(' +36 h ' M&7& h &w M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +89 h '\  .t\Elbow.seek CC M&p(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'( +416 h ( A(~& h &,9 A(b& h &,9 M&=& h &ӷ M&-% h % .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +142 h (w M&!& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &[' +93 h '  .t\Elbow.hold M& , +6 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &H1 +150 h 1 M&^% h %  A(& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M& '7 +20 h '7 M&3& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &E' +22 h ' A(<% h % ."downElevator"\SE.seek :down A(;'X +100 (&% h %S M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%a  + A(6 +112 h  P%w% h % .t\PS.seek 0.499ml P%K' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%? +38 h .t\PRV.dial :air P%k  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%W  +37 h .t\CBV.dial AIV C'\\  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%i  +19 h  P%{% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %>' +250 h ' .t\PRV.dial :waste2 P%| @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&/1 +2 h 1 M&& h &w M&% h %\  .t\Elbow.seek FlushPuck::Garage M&*(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&%/ +150 h / M&Q& h &- M&X% h %η .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +91 h ' .t\CC.seek :closed C'U(W +416 h (W C'& h &W .tHand.open M&81 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'h& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%}  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%D  +75 h  .tdelay 2 seconds +200 P%'% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%g +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%y  +38 h  .tdelay 2 seconds +200 P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %+'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%O  +75 h .tdelay 2 seconds +200 P%v% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%G'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%@  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%j  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%q +75 h .t\PTV.dial :PRV,avoiding: :puck P%S @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%u' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%_' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%2  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%I +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%z  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %e' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'. & +50 h & .t\AWV.select :waste C' +7 P%T% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %('nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'0 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %5' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%s' +2610 h ' .t\PSV.dial :PML P%l +38 h .t\PS.seek 0ml P%c' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'f& ."MFBPCR"\ASV.dial :SPEdelivery F* @@T h &W F*\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%7 +22 h  ."MFBPCR"delay 20 seconds +91 h P%N% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%C' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*o I +128 h I F*A% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *n'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%1' +1597 h ' .t\PSV.dial PSR P%x +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%"' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%9' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*$  +22 h ' .t\PSV.dial PSR P%L +21 h  F*:% h %6 ."MFBPCR"\AS.seek 0.329ml F*~'ͯ +92 h P%b% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%=' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*- % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%R' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*p  +22 h  F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *^' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%3' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*<% h % ."MFBPCR"\AS.seek 0.469ml F*Y' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%&' +2610 h ' .t\PSV.dial PSR P%4 +113 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%a' +740 h ' ."MFBPCR"delay 5 seconds +500 F*w9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*E n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*;% h % ."MFBPCR"\AS.seek 0.041ml F*]'% +336 h ' .t\PSV.dial PSR P%P +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%?' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%k +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%W' +2610 h ' .t\PSV.dial PSR P%\\ +113 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%{  +38 h  .t\PS.seek 0ml P%K' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*d I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b * 'V +850 h ' .t\PSV.dial :air P%%  +113 h  .t\PS.seek 2.5ml P%Q' +2609 h ' .t\PSV.dial :mixloop P%X +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %,' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*V  +43 h  F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *Z'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*8' +171 h ' .t\PSV.dial :mixloop P%) +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %h' +618 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*M'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*}  +85 h  F*D% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *'' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'g% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'y'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'm' +1626 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'O  +37 h  +113  .t\CBV.dial :waste1 C'v @ +38 h @ .t\CS.seek 0ml C'+'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*@'ݏ +110 h '4 .t\CSV.dial CSR C'j  +38 h  C'G% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'q'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*S  +21 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*u'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'2' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'I'4 +2648 h '4 C'z& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.seek CC M&e' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'(( +416 h ( M&& h &ӷ M&5% h % .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &s(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\ 'η +91 h 'η .tHand.open M&71 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'o& h & M&A% h %\  A( % h %X A(B. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (C(; +200 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (x& h &; M&1& h &- M&F% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &"(M +166 h (M M&$& h &M A(:& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &L'x +90 h 'x A(b& h &; ."WCR"\SE.seek 18.71 Pucks below Top Plate A(n' +77 h ' A(9& h &; M&-& h &M ."WCR"\Elbow.seek 5 M&~(M +6 h (M M&& h &M A(p& h &; ."WCR"\Forearm.seek 5 + 300 counts M&H' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &=/ +150 h / ."downElevator"\SE.seek :down M&& A(!'X h &M M&% h % ."WCR"\Forearm.seek :retract M&<'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&R(ӷ +143 h (ӷ M&^& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' +91 h ' ."WCR"\CC.seek :closed C'4(W +3 ."MFBPCR"\ATV.dial :waste2 F*[ % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +370 h (W ."WCR"Hand.open M&1 +150 h 1 M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *;' +30 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)Y+ +6 h + S)P% h % C'a& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'k  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'W +2327 h 'W #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'% h %4 S)% h %W S)d% h %W ."WCR"Intake.select :close S)0 +6 )`% h %W ."WCR"\SSV.dial :exhaust S)%  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %(A ."MFBPCR"\AS.seek 0.6ml F*Q'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*#'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*V% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*X % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2017h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*)  +11 h  F*Z% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*8  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *M'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*'  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*y  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*J' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*v % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*m% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*G'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +42 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226038 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'.  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'z @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +910 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)T% h % ."WCR"\CBV.dial between CSV and waste2b C'U  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'e' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '5  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'7  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'f' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'l  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'N  +38 h  C'A% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'o @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'F  +94 h  C'\ % h % ."WCR"\CSV.dial between air and CTV C'1 ` +56 h ` ."WCR"\CS.seek 7.288ml C':' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'b  '$  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '9'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'-  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 's' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'p  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'c  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'=' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'" @ +75 h @ +50 S)L% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'H ` +56 h ` ."WCR"\CS.seek 7.288ml C'^' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  '!  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'n  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '(' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'u  +151 h C'<& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M& % h %\  A(E% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (a(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (k& h &,9 M&P& h &ӷ M&&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &C(w +143 h (w M&3& h &w A( & h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &\\' +89 h ' A(Y& h &,9 ."WCR"\SE.seek 0.71 Pucks below Top Plate A({'K +254 h 'K M&W& h &w A(i& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &]' +25 h '  ."WCR"\Elbow.hold M&K, +6 h ,w ."WCR"\Elbow.reconfigure TightElbow A(/- h -+K M&$&.,(\ #b ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&% h %  A(|& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&`'7 +21 h '7 M&d& h &w A(>& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &%' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M&Q& h &w M&V% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +89 h '\  ."WCR"\Elbow.seek CC M&X(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&)/ +150 h / ."WCR"\CC.seek :open C'Z( +416 h ( A( & h &,9 A(*& h &,9 M&;& h &ӷ M&t% h % ."WCR"\Forearm.seek :retract M&}'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &8(w +142 h (w M&6& h &w A(& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &D' +93 h '  ."WCR"\Elbow.hold M&', +6 h ,w ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &g1 +150 h 1 M&M% h %  A(y& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&'7 +20 h '7 M&& h &w A(v& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &m' +23 h ' A(J% h % ."downElevator"\SE.seek :down A('X +100 (j% h %B M&O% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&G1 + A(6 + h 1 M&& h &w M&S% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +165 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&_/ +150 h / M&& h &- M&q% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +265 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'U& h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'5% h % .t\CSV.dial CSR C'e  +37 h  C'7% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'l% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'N'[ +463 h '[ .t\CSV.dial :waste C'A +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'f'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'B& h &W C'o% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'1% h %p .t\CS.seek 7ml C'x'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  'b  +37 h  +113  .t\CBV.dial :waste1 C': @ +76 h @ .t\CS.seek 0ml C'-'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'9& h &W C'p% h %4 .t\CSV.dial :flush C'c  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C's @ +38 h @ C'=% h %p .t\CS.seek 7ml C'"'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'L  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'H& h &W C'% h %4 .t\CSV.dial :flush C'[  +75 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +38 h @ C'n% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV C'u .t\CBV.dial CSV C'<  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'k +37 h .t\CS.seek 0ml C'P'4 +507 h '4 C'&& h &W C'3% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'Y'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'w% h %0 .t\CS.seek 5ml C'W'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'|'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'd& h &W C'>% h %4 .t\CSV.dial CSR C'Q  +150 h  C'V% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'\\'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'C' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ +113 h .t\CTV.dial :intake C']  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '`'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'%% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'K'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +710 h ' ."connect"\CSV.dial CTV C'#  .t\CTV.dial CSV C'4  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'*'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C';  +37 h  C't% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '6'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'X  +76 h  .t\CS.seek 0ml C'g'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'M  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'J '}  +38 h +75 .t\CTV.dial :air C'8  +151 h .t\CS.seek 0ml C'j'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'+ +38 h .t\CS.seek 0ml C'G'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'S% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'm% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +506 h '0 .t\CSV.dial :waste C'' +38 h .t\CS.seek 0ml C'_'4 +506 h '4 C'& h &W C'q% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +37 h @ C'I% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +506 h '0 .t\CSV.dial :waste C'U +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'% h %4 .t\CSV.dial :flush C'5  +38 h  .t\CS.seek 5ml C'e'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'% h %p .t\CS.seek 7ml C'('i +415 h 'i ."connect"\CSV.dial CTV C'l  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'A @ +38 h @ .t\CS.seek 0ml C'N'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'B& h &W C'o% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'1% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  +37 h  .t\CBV.dial :waste2b C'b  +76 h  .t\CS.seek 0ml C':'4 +766 h '4 #tDisinfecting external Intake with kill C'x& h &W C'9% h %4 .t\CSV.dial CSR C'p  +37 h  C'c% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'-'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C's' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '=  +38 h +75 .t\CTV.dial :intake C'L  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'"  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'[% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'^' +1372 h ' .t\CSV.dial CBV C'n +113 h .t\CS.seek 0ml C'!'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +6 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.seek CC M&E' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'( +416 h ( M&&& h &ӷ M&3% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &P(- +265 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Y'η +91 h 'η .tHand.open M&W1 +150 h 1 M&i% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&d1 +2 h 1 M&>& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &Q(- +7 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&{/ +150 h / M&]& h &- M&C% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' +91 h ' .t\CC.seek :closed C'K(W +416 h (W C'#& h &W .tHand.open M&41 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C';& h &W C't% h %4 .t\CSV.dial CSR C'*  +113 h  C'6% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'X% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'M +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'y'4 +2010 h '4 #t\Purging external Intake C'g& h &W C'% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'J' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 +76 h .t\CTV.dial :intake C'v  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'j'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'+  +76 h #t\Flushing upper manifold C'O& h &W C'% h %4 .t\CSV.dial :flush C'S  +75 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'''4 +506 h '4 C'a& h &W C'% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +37 h @ C'z% h %0 .t\CS.seek 5ml C'I'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'U'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&Z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'( +416 h ( M&e& h &ӷ M&l% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +265 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &o'η +91 h 'η .tHand.open M& 1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST21-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:37:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST21-Oct-18" +263637 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2017h25ml.pcr F*1% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*$  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +87 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *x'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +21 h F*p% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*='n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*L% h %n ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *['n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +31 h  ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*3'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2019h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*%% h %V ."MFBPCR"\AS.seek 0.5ml F*Q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*;  +22 h ."MFBPCR"delay 2 seconds +200 F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*6  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*!' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*X  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*}  +108 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*v n +21 h n ."MFBPCR"delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.6ml F*+'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*j  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2019h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*@  +21 h ."MFBPCR"\ATV.dial :air3 F*a % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*Z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*o% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*$'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2021h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ARV.dial :carrier F*[  +10 h  F*H% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*^ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*& n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*R I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.53ml F*u' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*W % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*> n +22 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*|'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*]' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*P  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\\% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2021h25ml.pcr F*Q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*# % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*X'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*!% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*}% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *y'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F*j % +86 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *''R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V @CEST1540072813.88 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2100h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ARV.dial :carrier F*Z  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *('Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*1 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*b  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*x n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*-'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*c  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*L' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*s'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*H % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2100h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*^  +22 h ."MFBPCR"\ATV.dial :air3 F*n % +86 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*~'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*0% h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*E'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*W  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*u% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*%'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*#% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2102h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*}  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*y% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*J n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*j  +21 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*S I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*? % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*m' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*@  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*''\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +21 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*7'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2102h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*5 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*)  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*K  +151 h F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *B' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*o n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *1'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*$% h % ."MFBPCR"\AS.seek 0.71ml F*x' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +21 h % ."MFBPCR"delay 3 seconds +300 F*p% h % H ."MFBPCR"\AS.seek 0.735ml F*c' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*-% h % Ű ."MFBPCR"\AS.seek 0.72ml F*=' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*" n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*L' +7161 h ' ."MFBPCR"\ATV.dial :waste F*[ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*s% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*<% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*k'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +21 h F*0% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*~' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*R n +86 h n ."MFBPCR"\ASV.dial :wasteR F*3  +22 h  ."MFBPCR"\AS.seek 0ml F*T'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *w'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*u % +108 h % F*E% h %V ."MFBPCR"\AS.seek 0.01ml F*i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F* % h % ."MFBPCR"\AS.seek 0.58ml F*>' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +21 h % ."MFBPCR"delay 3 seconds +300 F*d% h % q ."MFBPCR"\AS.seek 0.605ml F*{'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*C% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*Y' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*]  +21 h  ."MFBPCR"\AS.seek 0ml F*P'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*%  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*# n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +202 h 'R ."MFBPCR"\SPE.coast F*t  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*4'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*; n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *6' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*g  +43 h  F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*y  +65 h  F*M% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*J n +65 h n F*v% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *8' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*O  +42 h  F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*?% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*@% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *a' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*m I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *D': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *q'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*' n +150 h n ."MFBPCR"\AS.seek 0ml F*I'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*z  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*Z'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %0 ."MFBPCR"\AS.seek 0.985ml F*7' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*l% h % ."MFBPCR"\AS.seek 0.97ml F*e'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *('R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*1  +43 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *2' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F*f n +129 h n ."MFBPCR"\AS.seek 0ml F*$'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*9% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*p  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*"% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*s% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*^% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *.'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*3  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +87 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *W' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*|  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *C'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*t n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*;'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990939 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )` h  C' '* h  P% %4 h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ S)&\ P% *\ F*\ 9\ C' 2\ .t\SPE.coast P% 0\ F*\ 9\ *\ 9\ *6  h  .t\SPE.configure SPEconfig F*\ 2 *!% h %V .t\AS.configure ASconfig S)g% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'}& h & .t\CC.configure CCconfig S)#.0X 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:38:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #24) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'l& h & .tHand.open M&71 +150 h 1 M& & h &M M&e% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &o'η +91 h 'η .tHand.close M&/ +150 h / M&1& h &- M&N% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &f' +91 h ' .t\CC.seek :closed C'9(W +416 h (W C'F& h &W .tHand.open M&$1 +150 h 1 M&p% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C':% h %4 .t\CSV.dial CSR C'c  +150 h  C'"% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '-'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '=% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '['[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C's& h &W C'L% h %4 .t\CSV.dial :flush C'n  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'^ 'H  h +75 .t\CTV.dial :intake C'.  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'&& h &W C'0% h %4 .t\CSV.dial :flush C'k  +75 h  .t\CS.seek 5ml C'3'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +37 h @ C'T% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u +113 h .t\CTV.dial :air .\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +507 h '0 .t\CSV.dial :waste C'/ +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'w& h &W C'V% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'i'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'>% h %0 .t\CS.seek 5ml C'C'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'{'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\\'0 +507 h '0 .t\CSV.dial :waste C'] +75 h .t\CS.seek 0ml C'Y'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&P& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C't( +416 h ( M&& h &ӷ M& % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +264 h (- M&r& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&U1 +150 h 1 M&h% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 17:39:00CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:38:54CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*\ % #tLoading sh1 puck from tube 5 into Collection Clamp h %&K C'5& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&`% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(*% h %X A(4. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +6 F*6% h %&K ."readyMFB"\ATV.dial :waste2 F*! % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *g' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (}& h &; M&X& h &- M&y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &M(M +166 h (M M&& h &M A(J& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &v'x +90 h 'x A(& h &; .t\SE.seek 17.71 Pucks below Top Plate A(+'* +86 h '* A(8& h &; M&j& h &M .t\Elbow.seek 5 M&O(M +7 h (M M&S& h &M A(?& h &; .t\Forearm.seek 5 + 300 counts M&G' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&@& A('X h &M M&% h % .t\Forearm.seek :retract M&a'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&'(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&)1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)I% h % C'l& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast S)#.0X +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'09 h 9Td +100 C'.9 h 9kd +100 C'k9 h 9d +100 C' 9 h 9d +100 C'T9 h 9d +100 C'39 h 9d +100 C'u9 h 9d +100 C'R9 h 9d +100 C'E9 h 9 d +100 C'9 h 9 d +100 C'&9 h 96d +100 C'W9 h 9Ld +100 C'w9 h 9bd +100 C'V9 h 9xd +100 C'~9 h 9d +100 C' 9 h 9d +100 C'|9 h 9d +100 C'>9 h 9d +100 C'd9 h 9d +100 C'i9 h 9d +100 C'{9 h 9d +100 C']9 h 9(d +100 C'P9 h 9>d +100 C'C9 h 9Td +100 C'Q9 h 9id +100 C'/9 h 9d +100 C'%9 h 9d +100 C'\\9 h 9d +100 C'9 h 9d +100 C'x9 h 9d +100 C'99 h 9d +100 C' 9 h 9  d +100 C'r9 h 9 d +100 C'b9 h 9 *d +100 C'#9 h 9 ?d +100 C',9 h 9 Td +100 C'U9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'\ 9 h 9 0d +100 C'`9 h 9 4d +100 C'*9 h 9 8d +100 C'49 h 9 & h &,9 M&d& h &ӷ M&i% h % .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &](w +143 h (w M&P& h &w A(C& h &,9 .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 4 M&Q' +92 h '  .t\Elbow.hold M&/, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &%1 +150 h 1 M&\\% h %  A(& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&x'7 +21 h '7 M&9& h &w A( & h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &r' +22 h ' A(b% h % ."downElevator"\SE.seek :down A(#'X +100 (,% h %1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'\ ` +19 h ` .t\AIV.dial :bypass C'` { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&41 +2 h 1 M&6& h &w M&!% h %\  .t\Elbow.seek FlushPuck::Garage M&h(- +166 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&X'η +91 h 'η .tHand.close M&5/ +150 h / M&& h &- M&J% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C';(W +416 h (W C'8& h &W .tHand.open M&@1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%? +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%M' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%q' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%G  .t\CBV.dial PRV C'+  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%a  h .t\PS.seek 0ml P%O' +2610 h ' .t\PSV.dial :air P%)  +37 h  .t\PS.seek 2.5ml P%t' +2610 h ' .t\PSV.dial :mixloop P%I +38 h .t\PS.seek 0ml P%l' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%_' +2610 h ' .t\PSV.dial :mixloop P%e +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&g' +90 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'( +416 h ( M&N& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +265 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&K1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'p& h & M&% h %\  A(:% h %X A(c. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (F(; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (f& h &; M&m& h &- M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &(M +166 h (M M&[& h &M A(L& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &''x +89 h 'x A(D& h &; .t\SE.seek 16.71 Pucks below Top Plate A("'; +96 h '; A(H& h &; M&<& h &M .t\Elbow.seek 5 M&0(M +6 h (M M&.& h &M A( & h &; .t\Forearm.seek 5 + 300 counts M&T' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &R/ +150 h / ."downElevator"\SE.seek :down M&s& A(E'X h &M M&-% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.seek CC M&^(ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&W& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +91 h ' .t\CC.seek :closed C'Y(W +416 h (W .tHand.open M&31 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'|'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'>' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'd'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%i  +37 h  .t\PS.seek 2.5ml P%P' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%V  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%& +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%%  +151 h  .t\PS.seek :empty P%\\' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'9  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'b  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'{ ` +19 h ` .t\CSV.dial :air C'] @ +37 h @ .t\CS.seek 10ml C',' +2648 h ' .t\CSV.dial CTV C'U  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'Q @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'\  +151 h C'`& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&*% h %\  A(4% h %X A(6. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (!(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (x& h &,9 M&u& h &ӷ M&r% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &z(w +143 h (w M&}& h &w A($& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &#' +89 h ' A(o& h &,9 .t\SE.seek 2.71 Pucks below Top Plate A(/') +235 h ') M&5& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &J' +25 h '  .t\Elbow.hold M&, +7 h ,w .t\Elbow.reconfigure TightElbow A(X- h -+) M&$&.,(\ #b .t\SE.seek 3.04 Pucks below Top Plate A(A' +26 h ' M&j% h %  A(h& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&'7 +21 h '7 M& & h &w A(8& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &@' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(' +36 h ' M&?& h &w M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +89 h '\  .t\Elbow.seek CC M&v(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'+( +416 h ( A(& h &,9 A(a& h &,9 M&& h &ӷ M&)% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &I(w +142 h (w M&t& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &l' +93 h '  .t\Elbow.hold M&e, +6 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &_1 +150 h 1 M&% h %  A(B& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&'7 +21 h '7 M&7& h &w A(y& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(;% h % ."downElevator"\SE.seek :down A(N'X +100 (% h %  M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%K' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%p  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%:  +18 h  .t\PRV.dial :air P%c  +19 h  P%f% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %m' +251 h ' .t\PRV.dial :waste2 P%= @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'[& h & .tHand.open M&L1 +2 h 1 M&D& h &w M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&H(- +166 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&(/ +150 h / M&R& h &- M&s% h %η .t\Forearm.seek :retract M&-'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +91 h ' .t\CC.seek :closed C'"(W +416 h (W C'T& h &W .tHand.open M&01 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%3 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%k' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%F' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%|' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%> +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%i  +37 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %C'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%V  +75 h .tdelay 2 seconds +200 P%&% h %r .t\PS.seek 0.475ml P%%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%P  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%9  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %b' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%{ +75 h .t\PTV.dial :PRV,avoiding: :puck P%] @ +75 h @ .t\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial :air P%U  +75 h  .t\PS.seek 2.5ml P%,' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%\ ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%`  +75 h  .t\PS.seek 2.5ml P%*' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%4 +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%6  +76 h P%x% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %u' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'r & +50 h & .t\AWV.select :waste C' +6 P%}% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %$'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'o { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %^' +1312 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%5' +2610 h ' .t\PSV.dial :PML P% +37 h .t\PS.seek 0ml P%X' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'j& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'h& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass F*8  .t\PSV.dial PSR F* @@T P%? +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%#' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*z I +129 h I F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *J'6 +66 .t\PSV.dial :waste1 P%M  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%A' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%@ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*G  +22 h ' .t\PSV.dial PSR P%S +21 h  F*Y% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*) % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%q' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*v  +22 h  F*_% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%B +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +217 ."MFBPCR"\ATV.dial :AIV F*y I +43 h I F*;% h % ."MFBPCR"\AS.seek 0.469ml F*O' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%I +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%g +113 h P%l% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*!'% +336 h ' .t\PSV.dial PSR P%\\ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%p' +2609 h ' .t\PSV.dial PSR P% +113 h P%:% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%c' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%f  +37 h  .t\PS.seek 0ml P%K' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%= +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%m' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%L +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %D'k +2679 h 'k .t\PSV.dial :waste1 P%1  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %<' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*. I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *e'V +849 h ' .t\PSV.dial :air P%(  +113 h  .t\PS.seek 2.5ml P%R' +2610 h ' .t\PSV.dial :mixloop P%s +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %2' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %'' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*W  +43 h  F*H% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *-'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*T% h %Q ."MFBPCR"\AS.seek 0.01ml F*0' +170 h ' .t\PSV.dial :mixloop P%E +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*3 n +129 h n ."MFBPCR"\AS.seek :empty F*w'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*k% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'F  +37 h  C'>% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'i'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'|  'V  +38 h  +113  .t\CBV.dial :waste1 C'& @ +37 h @ .t\CS.seek 0ml C'C'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*P  +22 h ."MFBPCR"delay 3 seconds +300 F*%% h %_ ."MFBPCR"\AS.seek 0.434ml F*9'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'{'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*]  +22 h F*b% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*U'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*d n +107 h n F*Q% h %& ."MFBPCR"\AS.seek 0.092ml F*,'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'`' +1625 h ' ."connect"\CSV.dial CTV C'\  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'4'4 +2647 h '4 C'6& h &W #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +7 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&o/ +150 h / .t\CC.seek :open C'$( +416 h ( M&~& h &ӷ M&^% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +265 h (- M&j& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&?1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'z& h & M&/% h %\  A(M% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (#(; +200 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &; M&& h &- M&@% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &A(M +166 h (M M&G& h &M A(Y& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &S'x +89 h 'x A(a& h &; ."WCR"\SE.seek 15.71 Pucks below Top Plate A(J'L +107 h 'L A(& h &; M&)& h &M ."WCR"\Elbow.seek 5 M&(M +6 h (M M&& h &M A(v& h &; ."WCR"\Forearm.seek 5 + 300 counts M&_' +23 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down M&B& A(t'X h &M M&y% h % ."WCR"\Forearm.seek :retract M&;'\ +91 h '\  ."WCR"\Elbow.seek CC M&q(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +64 ."MFBPCR"\ATV.dial :waste2 F*g % +27 h ' ."WCR"\CC.seek :closed C'l(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +400 h (W ."WCR"Hand.open M&1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)O+ +6 h + S)\\% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*i  +87 h F*|% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *V'N +1026 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*& I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*P'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*% n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3301 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *C% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *]' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*b% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *9' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*d% h % ."MFBPCR"\AS.seek 0.279ml F*Q'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*U n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*\ % +43 h % F*`% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *6'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*x I +21 h I F*r% h %v ."MFBPCR"\AS.seek 0.059ml F*}'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*o % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*~% h % ."MFBPCR"\AS.seek 0.769ml F*^' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +21 h % ."MFBPCR"delay 3 seconds +300 F*j% h % q ."MFBPCR"\AS.seek 0.794ml F*h' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*?% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *z' +4416 h ' ."MFBPCR"\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.seek :empty F*M'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*8% h % ."MFBPCR"\AS.seek 0.579ml F*G' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %  ."MFBPCR"\AS.seek 0.604ml F*5'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*)% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*v n +86 h n ."MFBPCR"\ASV.dial :wasteR F*X  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*A % +108 h % F*S% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*I n +108 h n ."MFBPCR"\ASV.dial :wasteR F*_% h %  ."MFBPCR"\AS.seek 0.09ml F*J' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*g  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*t% h % ."MFBPCR"\AS.seek 0.12ml F*;' +626 h ' ."MFBPCR"\ATV.dial :air3 F*$ % +21 h % F*,% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*\\' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*7'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*#% h %X ."MFBPCR"\AS.seek 0.24ml F*O' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*:  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *c'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*! I +86 h I ."MFBPCR"\AS.seek 0ml F*L'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*='(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*1  +43 h  #"MFBPCR"\Swish #1 of 2 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *.'\  +1032 h '\  F*[% h %\  ."MFBPCR"\AS.seek 0.5ml F*('(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*m% h %(A ."MFBPCR"\AS.seek 0.6ml F*R'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*2'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*W% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *<' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*s % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +3472 h 'V ."MFBPCR"\SPE.coast F*+  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2117h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*T  +64 h  ."MFBPCR"\ARV.dial :carrier F*E  +10 h  F*-% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*0  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *w'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*>% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*i  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*& % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*V'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*P n +21 h n ."MFBPCR"delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*b  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*9  +43 h  ."MFBPCR"\ASV.dial :waste1 F*U  +65 h ."MFBPCR"\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226068 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'r  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'6 @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +910 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)}% h % ."WCR"\CBV.dial between CSV and waste2b C'"  +95 h  C'~% h % ."WCR"\CSV.dial between air and CTV C'n ` +18 h ` ."WCR"\CS.seek 7.288ml C'u' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'j  '^  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '*'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'h' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'/% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'z @ +75 h @ +50 S)M% h % ."WCR"\CBV.dial between CSV and waste2b C'@  +94 h  C'8% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'Y' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'a  'G  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C')  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '5' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'v  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'X  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'B' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'A @ +75 h @ +50 S)S% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'I% h % ."WCR"\CSV.dial between air and CTV C'_ ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'g  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 't'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'J  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '$' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C',  +150 h C';& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ( (,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&\\& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &#(w +142 h (w M&7& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &:' +89 h ' A(O& h &,9 ."WCR"\SE.seek 3.71 Pucks below Top Plate A(f' +224 h ' M&p& h &w A(c& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &!' +25 h '  ."WCR"\Elbow.hold M&K, +7 h ,w ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(L- h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(1' +27 h ' M&D% h %  A(=& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts + M&['7 +21 h '7 M&.& h &w A(m& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &(' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A( ' +37 h ' M&R& h &w M&W% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +89 h '\  ."WCR"\Elbow.seek CC M&s(ӷ +142 h (ӷ M&<& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ ."WCR"\Forearm.seek CC M&T' +91 h ' ."WCR"Hand.close M&E/ +150 h / ."WCR"\CC.seek :open C'-( +416 h ( A(e& h &,9 A(H& h &,9 M&N& h &ӷ M&3% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &0(w +143 h (w M&& h &w A(k& h &,9 ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.seek 4 M&' +92 h '  ."WCR"\Elbow.hold M&, +7 h ,w ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &>1 +150 h 1 M&w% h %  A(i& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts + M&|'7 +21 h '7 M&F& h &w A(&& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(%'X +100 ( % h % M&V% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +90 h '\  +21 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'C& h & .tHand.open M&1 +2 A(6 h 1 M&b& h &w M&]% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&Q'η +91 h 'η .tHand.close M&\ / +150 h / M&d& h &- M&{% h %η .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +265 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'}(W +415 h (W C'"& h &W .tHand.open M&~1 +150 h 1 M&n% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'^& h &W C'j% h % .t\CSV.dial CSR C'u  +38 h  C'?% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'*'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'/ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'h'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'z% h %4 .t\CSV.dial :flush C'M  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '@'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  'G  +37 h  +113  .t\CBV.dial :waste1 C'Y @ +75 h @ .t\CS.seek 0ml C')'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'v% h %4 .t\CSV.dial :flush C'X  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C'% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'S  +37 h  .t\CBV.dial :waste2b C'y  +76 h  .t\CS.seek 0ml C'I'4 +765 h '4 #t\Flushing upper manifold C'_& h &W C'B% h %4 .t\CSV.dial :flush C'g  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'J% h %p .t\CS.seek 7ml C't'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; ',  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'\\'4 +507 h '4 C'& h &W C'7% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'O'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +38 h @ C'% h %0 .t\CS.seek 5ml C'p'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +507 h '0 .t\CSV.dial :waste C'D +75 h .t\CS.seek 0ml C'='4 +506 h '4 #tDisinfecting external Intake with mfbkill C'.& h &W C'm% h %4 .t\CSV.dial CSR C'R  +151 h  C'W% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C':'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'#' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f +113 h .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'<' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'+  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '['4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'1  +113 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'K'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'2  'l  +37 h  +113  .t\CBV.dial :waste1 C'e @ +38 h @ .t\CS.seek 0ml C'H'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'N  +37 h  C'3% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +709 h ' ."connect"\CSV.dial CTV C'T  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C's  +75 h  .t\CS.seek 0ml C'>'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'w  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'0  +151 h .t\CS.seek 0ml C' '4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'C +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'b& h &W C']% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'9% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'%'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'\ '4 +506 h '4 C'd& h &W C'{% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.seek 3ml C'Q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'4% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'x'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +507 h '0 .t\CSV.dial :waste C'" +75 h .t\CS.seek 0ml C'~'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'n& h &W C'^% h %4 .t\CSV.dial :flush C'j  +37 h  .t\CS.seek 5ml C'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C'% h %p .t\CS.seek 7ml C'$'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'*  '  +38 h  +113  .t\CBV.dial :waste1 C'/ @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'z% h %4 .t\CSV.dial :flush C'M  +75 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV C'a  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'G  +76 h  .t\CS.seek 0ml C'Y'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'% h %4 .t\CSV.dial CSR C'v  +37 h  C'X% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C')'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'5' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +37 h +76 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'y' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'A  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '_'4 +607 h '4 .t\CSV.dial CSR C'B  +112 h  C'g% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'I'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1373 h ' .t\CSV.dial CBV C'J +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&7% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &\\(- +264 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&p1 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&.1 +2 h 1 M&m& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &R(- +7 h (- M&W& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&f/ +150 h / M&!& h &- M&#% h %η .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &[' +91 h ' .t\CC.seek :closed C'K(W +416 h (W C'2& h &W .tHand.open M&l1 +150 h 1 M&e% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'N& h &W C'3% h %4 .t\CSV.dial CSR C'H  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'T'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 's% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'k'[ +463 h '[ .t\CSV.dial :waste C'w +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'i'4 +2010 h '4 #t\Purging external Intake C'>& h &W C'F% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'0 +75 h .t\CTV.dial :intake C'&  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C' '4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'C  +75 h #t\Flushing upper manifold C'V& h &W C'b% h %4 .t\CSV.dial :flush C']  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C' % h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'| +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +506 h '0 .t\CSV.dial :waste C'% +38 h .t\CS.seek 0ml C''4 +506 h '4 C' & h &W C'd% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.seek 3ml C'P'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'6% h %0 .t\CS.seek 5ml C'4'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Q'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'x +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&-& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +6 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'( +416 h ( M&u& h &ӷ M&*% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +265 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&M1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +90 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST22-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:40:45CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST22-Oct-18" +263668 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2117h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*a  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*v% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*A  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +64 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *g'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*t  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2119h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*1  +11 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*R'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *2'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*N  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *H'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*s  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*k  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*F'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*& n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*]' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2119h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*U  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *{'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*\ % h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*z% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*a'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2121h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ARV.dial :carrier F*g  +11 h  F*_% h %V ."MFBPCR"\AS.seek 0.5ml F*I'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *,'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *}'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*q I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*;' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*p % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*.'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*m n +21 h n ."MFBPCR"delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.6ml F*='\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*f% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*1'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*D % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2121h25ml.pcr F*R% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*+  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *N'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +21 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*s'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*>  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*C  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V @CEST1540159221.90 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2200h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F*-  +11 h  F*<% h %V ."MFBPCR"\AS.seek 0.5ml F*E'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*u n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*n  +22 h ."MFBPCR"delay 2 seconds +200 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F* ' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*G  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*h% h %< ."MFBPCR"\AS.seek 0.594ml F*v'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*)'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*A  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2200h25ml.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*J % +87 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*7% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*p  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *c'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*f  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*#% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*1'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*2% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2202h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*k  +10 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*C  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*] I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*|% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*9' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*U  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +21 h  ."MFBPCR"delay 7 seconds +700 F*d% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F*P'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.65ml F*x' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2202h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*j @@T ."MFBPCR"\ASV.dial :SPEdelivery F*E  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*K  +150 h F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*n n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*M  +43 h  F*~% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*z n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*`'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +42 h % F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *@' +424 h ' ."MFBPCR"\ATV.dial :air3 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*a% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h % H ."MFBPCR"\AS.seek 0.735ml F*X' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*)% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*A n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*y% h % zG ."MFBPCR"\AS.seek 0.365ml F*S' +7161 h ' ."MFBPCR"\ATV.dial :waste F*g n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*B % +43 h % F*5% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*,% h % ."MFBPCR"\AS.seek 0.579ml F*I' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*o% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*t' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*q n +86 h n ."MFBPCR"\ASV.dial :wasteR F*7  +21 h  ."MFBPCR"\AS.seek 0ml F*}'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*; % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*m' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h % q ."MFBPCR"\AS.seek 0.605ml F*f'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*#% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*O' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*!  +21 h  ."MFBPCR"\AS.seek 0ml F*\\'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *R'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*(  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *+' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*2 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *1'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +202 h 'R ."MFBPCR"\SPE.coast F*3  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*l'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*N n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*[  +43 h  F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*T I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*>  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *k': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*i  +65 h  F*w% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *F'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*&% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *0' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*C I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*V  +43 h  F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *|': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*b  +65 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*U% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*9 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*d  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*\ n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *{'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*4'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*6  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*P % +42 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +21 h F*x% h % ."MFBPCR"\AS.seek 0.96ml F*-'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +21 h % ."MFBPCR"delay 3 seconds +300 F*E% h %0 ."MFBPCR"\AS.seek 0.985ml F*?' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F**% h % ."MFBPCR"\AS.seek 0.97ml F*u'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*n I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *~'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *z'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *$'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *`' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*@' +9880 h ' ."MFBPCR"\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.seek 0ml F*a'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *)'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*5% h %n ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*J  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*7  +32 h  ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*c'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*m'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*=  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *#'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *+'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *1'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*e % +107 h % ."MFBPCR"\AS.seek 0ml F*N'V +2548 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990137 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )[ h  C' 'H h  P% %l h  F* *T h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)>% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:41:45CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #25) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&?1 +150 h 1 M&n& h &M M&u% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &M(- +166 h (- M&~& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &z'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &h' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'@& h &W .tHand.open M&a1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'Y% h %4 .t\CSV.dial CSR C'X  +150 h  C'A% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'g'[ +463 h '[ .t\CSV.dial :waste C'y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'B'4 +2009 h '4 #t\Purging external Intake C'5& h &W C'S% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'_ '  h +75 .t\CTV.dial :intake C'r  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'I  +75 h #t\Flushing upper manifold C'& h &W C'o% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'7'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +37 h @ C'}% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +507 h '0 .t\CSV.dial :waste C'L +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'W% h %4 .t\CSV.dial :flush C'.  +38 h  .t\CS.seek 3ml C'c'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'm% h %0 .t\CS.seek 5ml C'#'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'f'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'O'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&\\& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.seek CC M&2' +91 h ' .tHand.close M&+/ +150 h / .t\CC.seek :open C'3( +416 h ( M&& h &ӷ M&e% h % .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&Z1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 17:41:50CEST26-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:41:45CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*"% h %&K #tLoading sh1 puck from tube 5 into Collection Clamp #"readyMFB"Readying the MFB C'j& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&[% h %\  A(H% h %X A(l. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (T(; +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *>' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (& h &; M&s& h &- M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &w(M +166 h (M M&k& h &M A(& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &&'x +90 h 'x A(F& h &; .t\SE.seek 14.71 Pucks below Top Plate A(C'] +116 h '] A(0& h &; M& & h &M .t\Elbow.seek 5 M&V(M +6 h (M M&]& h &M A( & h &; .t\Forearm.seek 5 + 300 counts M&b' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down A('X M&U& h &M M&% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M&(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'E(W +416 h (W .tHand.open M&<1 +150 h 1 M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)P+ +6 h + S)4% h % C'*& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 91d +100 C'9 h 9J d +100 C'?9 h 9bd +100 C'9 h 9zd +100 C'9 h 9d +100 C'x9 h 9d +100 C'9 h 9 d +100 C' 9 h 9$d +100 C'`9 h 9(d +100 C'M9 h 9 ,d +100 C'$9 h 9$0d +100 C'-9 h 9<4d +100 C'z9 h 9S8d +100 C'v9 h 9kd +100 C'o9 h 9Td +100 C'r9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9d +100 C'79 h 9d +100 C';9 h 9d +100 C'q9 h 9d +100 C'9 h 9 d +100 C'B9 h 9 d +100 C'9 h 96d +100 C'p9 h 9Ld +100 C'9 h 9bd +100 C'W9 h 9xd +100 C't9 h 9d +100 C'9 h 9d +100 C'=9 h 9d +100 C'm9 h 9d +100 C'.9 h 9d +100 C'c9 h 9d +100 C'f9 h 9d +100 C'!9 h 9(d +100 C'\\9 h 9>d +100 C'#9 h 9Td +100 C'R9 h 9id +100 C'L9 h 9d +100 C'(9 h 9d +100 C':9 h 9d +100 C'+9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'e9 h 9  d +100 C''9 h 9 d +100 C'G9 h 9 *d +100 C'29 h 9 ?d +100 C'89 h 9 Td +100 C'Z9 h 9 j d +100 C'9 h 9 $d +100 C'D9 h 9 (d +100 C'19 h 9 ,d +100 C'"9 h 9 0d +100 C'[9 h 9 4d +100 C'H9 h 9 8d +100 C'l9 h 9  @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'? @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'x  +19 h C'% h %4 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'`  +38 h C'M% h %p .t\CS.seek 7ml C'$'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'- @ +37 h @ .t\CS.seek 10ml C'z' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'v ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M&Y% h %\  A(X% h %X A(@. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (a(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (h& h &,9 M&9& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &)(w +143 h (w M&y& h &w A(g& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &5' +89 h ' A(S& h &,9 .t\SE.seek 4.71 Pucks below Top Plate A(J' +214 h ' M&A& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &_' +26 h '  .t\Elbow.hold M&I, +6 h ,w .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(,- h -+ .t\SE.seek 5.04 Pucks below Top Plate A(O' +27 h ' M&o% h %  A(r& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&'7 +20 h '7 M&& h &w A(}& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &7' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(;' +37 h ' M&q& h &w M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&W' +90 h ' .tHand.close M&t/ +150 h / .t\CC.seek :open C'( +416 h ( A(=& h &,9 A(m& h &,9 M&.& h &ӷ M&c% h % .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &!(w +143 h (w M&\\& h &w A(#& h &,9 .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 4 M&R' +92 h '  .t\Elbow.hold M&L, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &(1 +150 h 1 M&:% h %  A(+& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&'7 +21 h '7 M&& h &w A(e& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &'' +22 h ' A(G% h % ."downElevator"\SE.seek :down A(2'X +100 (8% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +12 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'D @ +2 A(6 +17 h @ .t\CS.seek 10ml C'1' +7 h ' .t\CSV.dial between air and CTV C'" ` +18 h ` .t\AIV.dial :bypass C'[ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&l1 +2 h 1 M&& h &w M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&s'η +91 h 'η .tHand.close M&j/ +150 h / M&k& h &- M&% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +265 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &]' +90 h ' .t\CC.seek :closed C'N(W +416 h (W C'0& h &W .tHand.open M&U1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%w' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%{' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%d' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%b  C'C  h +75 .t\PSV.dial :mixloop P%V +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%4 +38 h .t\PS.seek 0ml P%*' +2610 h ' .t\PSV.dial :air P%|  +37 h  .t\PS.seek 2.5ml P%\ ' +2610 h ' .t\PSV.dial :mixloop P%u +38 h .t\PS.seek 0ml P%P' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'~( +416 h ( M&& h &ӷ M&x% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&-1 +150 h 1 M&n% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'v& h & M&% h %\  A(Y% h %X A(X. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (@(; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (h& h &; M&9& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &y(M +166 h (M M&g& h &M A(S& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &'x +90 h 'x A(& h &; .t\SE.seek 13.71 Pucks below Top Plate A(A'n +125 h 'n A(& h &; M&,& h &M .t\Elbow.seek 5 M&o(M +6 h (M M&r& h &M A(& h &; .t\Forearm.seek 5 + 300 counts M&}' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &q/ +150 h / ."downElevator"\SE.seek :down A('X M&5& h &M M&)% h % .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.seek CC M&_(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'O(W +416 h (W .tHand.open M&71 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'B  +150 h  .t\CSV.dial CTV C'E  +19 h  .t\CS.seek 5ml C'='p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'm' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'.'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%c  +37 h  .t\PS.seek 2.5ml P%\\' +2610 h ' .t\PSV.dial :mixloop P%# +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%W  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%(  +151 h  .t\PS.seek :empty P%:' +2609 h ' .t\CBV.dial :waste1 C'+ @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'e @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'G  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'f ` +19 h ` .t\CSV.dial :air C'! @ +37 h @ .t\CS.seek 10ml C'8' +2648 h ' .t\CSV.dial CTV C'Z  +37 h  .t\CS.seek 0ml C'D'4 +2647 h '4 .t\CBV.dial :waste1 C'" @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'[  +151 h C'H& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&l% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (R(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&;& h &ӷ M&'% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &6(w +143 h (w M&& h &w A(a& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &2' +89 h ' A(z& h &,9 .t\SE.seek 5.71 Pucks below Top Plate A(L' +205 h ' M&j& h &w A(k& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &' +25 h '  .t\Elbow.hold M&, +7 h ,w .t\Elbow.reconfigure TightElbow A(s- h -+ M&$&.,(\ #b .t\SE.seek 6.04 Pucks below Top Plate A(/' +27 h ' M& % h %  A(& h &,9 .t\Forearm.force -20 M&5 .t\Forearm.seek 4 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &F'7 +21 h '7 M&& h &w A(0& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &U' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(' +36 h ' M& & h &w M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +90 h '\  .t\Elbow.seek CC M&&(ӷ +142 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C'C( +416 h ( A(V& h &,9 A( & h &,9 M&d& h &ӷ M&<% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &4(w +143 h (w M&3& h &w A(|& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &*' +92 h '  .t\Elbow.hold M&u, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\ 1 +150 h 1 M&T% h %  A(& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&>'7 +21 h '7 M&?& h &w A(i& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(N% h % ."downElevator"\SE.seek :down A('X +100 (x% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%M  +2 A(6 +111 h  P%Q% h % .t\PS.seek 0.499ml P%-' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%n +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%v  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%Y  +19 h  .t\PRV.dial :air P%X  +19 h  P%h% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %9' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb % ' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&S1 +2 h 1 M&& h &w M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&$/ +150 h / M&q& h &- M&5% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'}(W +416 h (W C'A& h &W .tHand.open M&o1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +91 h '\  C'~& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%7 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%I  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%B  +75 h  .tdelay 2 seconds +200 P%E% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%@' +1094 h ' .t\PSV.dial :air P%1  +38 h  .t\PS.seek 0ml P%=' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%m +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%c  +38 h  .tdelay 2 seconds +200 P%P% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %#'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%W  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%(' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%\\  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%+'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%e  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %G' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%f +76 h .t\PTV.dial :PRV,avoiding: :puck P%! @ +75 h @ .t\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%8' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%" +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%[' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%H  +76 h  .t\PS.seek 2.5ml P%l' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %;' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'' & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %a'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'z { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +1313 h ' .t\PSV.dial :air P%j  +38 h  .t\PS.seek 2.5ml P%k' +2609 h ' .t\PSV.dial :PML P%t +38 h .t\PS.seek 0ml P%s' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 ' & h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*0  P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*6 I +129 h I F*L% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +65 .t\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%F +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%/' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*b  +22 h ' .t\PSV.dial PSR P%] +20 h  F*O% h %6 ."MFBPCR"\AS.seek 0.329ml F*V'ͯ +93 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%d' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*< % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%|  +38 h  .t\PS.seek 0ml P%{' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*&  +22 h  F*\ % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *T' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%?' +217 ."MFBPCR"\ATV.dial :AIV F*i I +43 h I F*N% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%x' +2609 h ' .t\PSV.dial PSR P% +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%M' +740 h ' ."MFBPCR"delay 5 seconds +500 F*Q9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*R'% +337 h ' .t\PSV.dial PSR P%: +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%n' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%v' +2610 h ' .t\PSV.dial PSR P% +113 h P%Y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%h  +38 h  .t\PS.seek 0ml P%-' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%9' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%S +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %,' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*r I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *u'V +850 h ' .t\PSV.dial :air P%$  +113 h  .t\PS.seek 2.5ml P%q' +2610 h ' .t\PSV.dial :mixloop P%5 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %`' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*p  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *)'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*C I +43 h I F*A% h %Q ."MFBPCR"\AS.seek 0.01ml F*o' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %~' +618 h ' ."MFBPCR"\ATV.dial :waste F*7 n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*I  +86 h  F*B% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *E' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *1'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'@  +38 h  C'm% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'c'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'P' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  '=  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'#'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*\\  +21 h ."MFBPCR"delay 3 seconds +300 F*(% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'e  +38 h  C'+% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'f'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*!  +21 h F*G% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*. n +108 h n F*"% h %& ."MFBPCR"\AS.seek 0.092ml F*8'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'[  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +6 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'a( +416 h ( M&j& h &ӷ M&_% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &k(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'6& h & M&L% h %\  A(w% h %X A(%. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (2(; +201 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h &; M&^& h &- M&U% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &/(M +166 h (M M&b& h &M A(O& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &]'x +89 h 'x A( & h &; ."WCR"\SE.seek 12.71 Pucks below Top Plate A(' +136 h ' A(& h &; M&<& h &M ."WCR"\Elbow.seek 5 M&V(M +7 h (M M&|& h &M A(&& h &; ."WCR"\Forearm.seek 5 + 300 counts M&\ ' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &d/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down A(3'X M&& h &M M&i% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.seek CC M&{(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&T& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &?' +35 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +13 h ' ."WCR"\CC.seek :closed C'x(W +416 h (W ."WCR"Hand.open M&1 +131 h 'R ."MFBPCR"\ATV.dial :air2 F*>  +19 h 1 M&Q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +89 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S):% h % C'M& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.seek 0.23ml F*:' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +21 h F*2% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Y n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *X'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*n @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*R I +86 h I ."MFBPCR"\AS.seek 0ml F*S'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *r'\  +1031 h '\  F*g% h %\  ."MFBPCR"\AS.seek 0.5ml F*$'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*9% h %(A ."MFBPCR"\AS.seek 0.6ml F*q'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*`'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*p% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *,' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*5 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +3472 h 'V ."MFBPCR"\SPE.coast F*C  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2217h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*A  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*E  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*m% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*c  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*W% h % ."MFBPCR"\AS.seek 0.53ml F*D' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +107 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*G  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +64 h ."MFBPCR"\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*[% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226098 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C''  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +909 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'j% h % ."WCR"\CSV.dial between air and CTV C'J ` +19 h ` ."WCR"\CS.seek 7.288ml C';' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '_  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'l'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C't  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'L% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'%' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'6 @ +75 h @ +50 S)w% h % ."WCR"\CBV.dial between CSV and waste2b C'U  +94 h  C'0% h % ."WCR"\CSV.dial between air and CTV C'^ ` +57 h ` ."WCR"\CS.seek 7.288ml C'O' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'b  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'<  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'&  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C's  +38 h  C'|% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'd' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'/ @ +75 h @ +50 S)]% h % ."WCR"\CBV.dial between CSV and waste2b C'i  +95 h  C'4% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'T' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'V  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '3'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '8' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'a  +151 h C'N& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&Q% h %\  A(>% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (M(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&:& h &ӷ M&*% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &2(w +142 h (w M&?& h &w A(Y& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &,9 ."WCR"\SE.seek 6.71 Pucks below Top Plate A(h' +195 h ' M&v& h &w A(X& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &R' +26 h '  ."WCR"\Elbow.hold M&-, +6 h ,w ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(S- h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(' +27 h ' M&% h %  A(& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&g'7 +20 h '7 M&r& h &w A(9& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &$' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A('w +37 h 'w M&q& h &w M&p% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +89 h '\  ."WCR"\Elbow.seek CC M&5(ӷ +143 h (ӷ M&,& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.seek CC M&A' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C')( +415 h ( A(u& h &,9 A(& h &,9 M&& h &ӷ M&7% h % ."WCR"\Forearm.seek :retract M&I'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &o(w +143 h (w M& & h &w A(& h &,9 ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.seek 4 M&B' +92 h '  ."WCR"\Elbow.hold M&E, +7 h ,w ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &m1 +150 h 1 M&% h %  A(c& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&W'7 +20 h '7 M&@& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &P' +22 h ' A(D% h %w ."downElevator"\SE.seek :down A(('X +100 (e% h % M&=% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'#& h & .tHand.open M&+1 +2 h 1 M&G& h &w M&!% h %\  .t\Elbow.seek FlushPuck::Garage M&1(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&"'η +91 h 'η .tHand.close M&[/ +150 h / M&.& h &- M&f% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'}& h &W .tHand.open M&j1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'_& h &W C' % h % .t\CSV.dial CSR C';  +37 h  C' % h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'l'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 't% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'L +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'%& h &W C'6% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'^% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'b  '  +38 h  +112  .t\CBV.dial :waste1 C'O @ +75 h @ .t\CS.seek 0ml C'<'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'&% h %4 .t\CSV.dial :flush C's  +75 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'd% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  +38 h  .t\CBV.dial :waste2b C'i  +75 h  .t\CS.seek 0ml C'4'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'\ % h %p .t\CS.seek 7ml C'3'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a 'N  +38 h +75 .t\CTV.dial :air C'Q  +151 h .t\CS.seek 0ml C'>'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C':'4 +506 h '4 C'*& h &W C'?% h %4 .t\CSV.dial :flush C'Y  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'v% h %0 .t\CS.seek 5ml C'X'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'r& h &W C'9% h %4 .t\CSV.dial CSR C'q  +150 h  C'p% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'2' +416 h ' ."connect"\CSV.dial CBV C'h .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'R  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C',' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'C  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'g'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'$% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'-'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  'x  +38 h  +113  .t\CBV.dial :waste1 C'u @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'7% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'A  +37 h  .t\CBV.dial :waste2b C'5  +76 h  .t\CS.seek 0ml C'm'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'c% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '@'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I 'D  +38 h +75 .t\CTV.dial :air C'o  +151 h .t\CS.seek 0ml C'e'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +506 h '0 .t\CSV.dial :waste C'# +38 h .t\CS.seek 0ml C'+'4 +506 h '4 #t\Flushing upper manifold C'G& h &W C'!% h %4 .t\CSV.dial :flush C'B  +38 h  .t\CS.seek 5ml C'W'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P +113 h .t\CTV.dial :air .\CS.seek 0ml C'('4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +506 h '0 .t\CSV.dial :waste C'E +38 h .t\CS.seek 0ml C'['4 +507 h '4 C'.& h &W C'f% h %4 .t\CSV.dial :flush C'\\  +37 h  .t\CS.seek 3ml C'"'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'1'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C')'0 +506 h '0 .t\CSV.dial :waste C'} +76 h .t\CS.seek 0ml C'j'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'J& h &W C'_% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'y% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  't  +37 h  +113  .t\CBV.dial :waste1 C'L @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'%& h &W C'6% h %4 .t\CSV.dial :flush C'w  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'^% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'b  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'O'4 +766 h '4 #tDisinfecting external Intake with kill C'F& h &W C'% h %4 .t\CSV.dial CSR C'&  +38 h  C's% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'<'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'k' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'| 'd  +38 h +75 .t\CTV.dial :intake C']  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'i' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'/  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'V% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'4'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'T' +1372 h ' .t\CSV.dial CBV C'\ +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&N& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +415 h ( M&*& h &ӷ M&?% h % .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +265 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&X1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&r1 +2 h 1 M&9& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &q(- +6 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&h/ +150 h / M&R& h &- M&2% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +91 h ' .t\CC.seek :closed C'-(W +416 h (W C'`& h &W .tHand.open M&x1 +150 h 1 M&u% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'7% h %4 .t\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'A'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '5% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig C'#P d@b .t\CS.seek 0ml C'c'4 +2010 h '4 #t\Purging external Intake C'm& h &W C'@% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'o .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'e'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'#  +76 h #t\Flushing upper manifold C'=& h &W C'G% h %4 .t\CSV.dial :flush C'!  +75 h  .t\CS.seek 5ml C'B'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W +113 h .t\CTV.dial :air .\CS.seek 0ml C'P'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +506 h '0 .t\CSV.dial :waste C'( +38 h .t\CS.seek 0ml C'E'4 +507 h '4 C'M& h &W C'.% h %4 .t\CSV.dial :flush C'f  +37 h  .t\CS.seek 3ml C'\\'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'"'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'1'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'}'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'y( +416 h ( M&;& h &ӷ M&t% h % .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &L(- +264 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&w1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Extending 18tundeployv2 until completion of MFBPCR +263687 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2217h25ml.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*b  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*&% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +21 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*d'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*k'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*i  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *V'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*3  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F**% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +21 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F*v'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2219h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*$% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *`'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*u  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*5  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*m% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +108 h  ."MFBPCR"delay 7 seconds +700 F*c% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*e  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2219h25ml.pcr F*P% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*[% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*1% h %n ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*_  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*%  +32 h  ."MFBPCR"\ATV.dial :air3 F*J % +86 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*6% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*b'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*U% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2221h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*k  +43 h  ."MFBPCR"\ARV.dial :carrier F*V  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*4'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +21 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F** n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*>' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*{ I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*a' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*X % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*v% h % ."MFBPCR"\AS.seek 0.536ml F*Y'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*r'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*9 n +21 h n ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*:  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2221h25ml.pcr F*q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*C  +22 h ."MFBPCR"\ATV.dial :air3 F*` % +86 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*5'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*m  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*D% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *c'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*#  +32 h  ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*W% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %> ."MFBPCR"\AS.seek 0.835ml F*B'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*M% h %m ."MFBPCR"\AS.seek 0.82ml F*.'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *E'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1540245621.30 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2300h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*)  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*%  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*J  +22 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*l I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*6% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*^ n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.536ml F*b'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.6ml F*<'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.65ml F*]' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*d  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*/  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*i% h % \\ ."MFBPCR"\AS.seek 0.326ml F*k'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2300h25ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +21 h F*Q% h %Z ."MFBPCR"\AS.seek 0.81ml F*3'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +21 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*v  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.81ml F*$'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2302h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*@'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*#  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*e  +22 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*! I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*W% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*n % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*P% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*Z  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.536ml F*B'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +22 h  ."MFBPCR"delay 7 seconds +700 F*.% h %< ."MFBPCR"\AS.seek 0.594ml F*E'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*[ n +21 h n ."MFBPCR"delay 2 seconds +200 F*f% h %\  ."MFBPCR"\AS.seek 0.6ml F*\\'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*)  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2302h25ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*-  +151 h F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *%' +424 h ' ."MFBPCR"\ATV.dial :ASV F*J n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*w  +43 h  F*j% h % ."MFBPCR"\AS.seek 0.62ml F*l'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*6 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *^'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*H'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *U' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*b% h % ."MFBPCR"\AS.seek 0.71ml F*F' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h % H ."MFBPCR"\AS.seek 0.735ml F*s' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*<% h % Ű ."MFBPCR"\AS.seek 0.72ml F*d' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*/ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*i% h % zG ."MFBPCR"\AS.seek 0.365ml F*]' +7161 h ' ."MFBPCR"\ATV.dial :waste F*V n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*k% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*N% h % ."MFBPCR"\AS.seek 0.579ml F*4' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*z% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*3' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*{ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*?  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*a % +107 h % F*>% h %V ."MFBPCR"\AS.seek 0.01ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*Y% h % ."MFBPCR"\AS.seek 0.58ml F*9' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h % q ."MFBPCR"\AS.seek 0.605ml F*h'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*2% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*R  +22 h  ."MFBPCR"\AS.seek 0ml F*:'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*$  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *C' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*` n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +202 h 'R ."MFBPCR"\SPE.coast F*7  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*x'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*g  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*m  +43 h  F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*c  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *@'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*I n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *o' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*# I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*=  +43 h  F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *W': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*G  +65 h  F*n% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *P'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*Z% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *M' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*.  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *B': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*[ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *f'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*E n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*\\ % +43 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*)'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F* ' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*t% h % ."MFBPCR"\AS.seek 0.97ml F*;'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*J I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *j'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *6'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *8'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*^  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *H' +9987 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*U' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*b'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*&  +22 h ."MFBPCR"\ATV.dial :air3 F*F % +43 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +21 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*]'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*k% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*N  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +31 h  ."MFBPCR"\ATV.dial :air3 F*3 % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*>% h % ."MFBPCR"\AS.seek 0.81ml F*v'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +21 h F*r% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*u % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tundeployv2 assays .GoodNews.suppressMail "{1 => 22, 2 => 22, 3 => 22, 4 => 9, 5 => 13, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:42:51CEST26-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tundeployv2 assays" #\Mission runtime was 1:11:30.962384 #-> exit #m\EXIT @1537977048.08CEST @1537977912.87CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeployv2dil" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"p/home/esp/esp2/mission/18tundeployv2dil.rb:44: syntax error, unexpected tIDENTIFIER, expecting kEND\ logPhaseStart :Superphase with 1:10 dilution\ ^\ /home/esp/esp2/mission/18tundeployv2dil.rb:46: syntax error, unexpected ',', expecting kEND\ mfb([$mfbVol, $wcrVol]), $bacmfb.dup.update(:lysateCoc...\ ^\ /home/esp/esp2/mission/18tundeployv2dil.rb:46: syntax error, unexpected ')', expecting kEND\ ...eMFBlysate; diluteMFBlysate})) {hotBleach}\ ^\ /home/esp/esp2/mission/18tundeployv2dil.rb:148: syntax error, unexpected $end, expecting kEND #-> execute "18tundeployv2dil" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"p/home/esp/esp2/mission/18tundeployv2dil.rb:44: syntax error, unexpected tIDENTIFIER, expecting kEND\ logPhaseStart :Superphase with 1:10 dilution\ ^\ /home/esp/esp2/mission/18tundeployv2dil.rb:46: syntax error, unexpected ',', expecting kEND\ mfb([$mfbVol, $wcrVol]),$bacmfb.dup.update(:lysateCock...\ ^\ /home/esp/esp2/mission/18tundeployv2dil.rb:46: syntax error, unexpected ')', expecting kEND\ ...eMFBlysate; diluteMFBlysate})) {hotBleach}\ ^\ /home/esp/esp2/mission/18tundeployv2dil.rb:148: syntax error, unexpected $end, expecting kEND #-> execute "18tundeployv2dil" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"b/home/esp/esp2/mission/18tundeployv2dil.rb:148: syntax error, unexpected $end, expecting kEND #-> execute "18tundeployv2dil" .GoodNews.suppressMail "Starting 18tundeployv2dil w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:05:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployv2dil begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: phasewithdil\\n/home/esp/esp2/mission/18tundeployv2dil.rb:113\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:14:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ % *\ C' -\ P% 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& h & S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:14:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2dil phase phasewithdil" #\Phase [phasewithdil] #==================> STARTED Superphasewdil (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 18:14:11CEST26-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:14:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %8 .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*I  +11 h  ."readyMFB"\ASV.dial :waste1 F*H  h +912 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'K% h %4 S)J% h % S)M% h % .tIntake.select :close S)0 +6 )N% h % .t\SSV.dial :exhaust S)O  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9Td +100 C'99 h 9Xd +100 C'I9 h 9(\\d +100 C'H9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'G9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 & h &ӷ &( .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A(I'Z .t\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(G& h &( A(R& h &( M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +142 h ( M&W& h & A(S& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'C +93 h 'C .t\Elbow.hold M&T, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %C A(]& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&\\'o +20 h 'o M&^& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +23 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ +2 A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +18 h ` .t\AIV.dial :bypass C'e { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P%| +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'\  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&,1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(1. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A()& h &  .t\SE.seek 19.71 Pucks below Top Plate A( ' +66 h ' A(&& h &  M&8& h & .t\Elbow.seek 2 M&=( +6 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &2/ +150 h / ."downElevator"\SE.seek :down M&B& A(:'X h & M&D% h %8 .t\Forearm.seek :retract M&E'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&F(ӷ +143 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +151 h  .t\PS.seek :empty P%Y' +2609 h ' .t\CBV.dial :waste1 C'] @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +37 h @ .t\CS.seek 10ml C'c' +2648 h ' .t\CSV.dial CTV C'Z  +37 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +151 h C'I& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h &( M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +143 h ( M&o& h & A(b& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &A' +89 h ' A(6& h &( .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +264 h '\\ M&r& h & A(C& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'C +26 h 'C .t\Elbow.hold M&a, +6 h , .t\Elbow.reconfigure TightElbow A(j- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %C A(p& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&u'o +20 h 'o M&O& h & A(t& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +415 h ( A(& h &( A( & h &( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +143 h ( M& & h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(q& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%,' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%1  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M&)& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&2& h &- M&B% h %η .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &9' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C' & h &W .tHand.open M&:1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  C'#& h &W #tRinsing MixLoop with GuSCN .\PSV.dial :mixloop,avoiding: PBV .\PTV.dial :PRVmixing,avoiding: :puck .\PRV.dial :spare1 P%J  +113 h P%N% h % .t\PS.seek 1.099ml P%'^ +1194 h '^ .tdelay 2 seconds +200 .\PRV.dial :air P%+  +113 h  .t\PS.seek 2.5ml P%>' +1497 h ' .t\PSV.dial :waste1,avoiding: PBV P%%  +151 h  .t\PS.seek :empty P%g' +2609 h ' #tDiluting Lysate in Syringe .\PSV.dial :PTV,avoiding: PBV P%G +189 h .t\PTV.dial :PRV,avoiding: :puck P%R @ +75 h @ .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial CBV P%U  +38 h .tdelay 2 seconds +200 .\PS.reconfigure PSslow1 .\PS.seek 0.1ml P%#A \ `xb %' +1053 h ' .tdelay 5 seconds +500 .\PRV.dial :spare1 P%S  +75 h .t\PS.reconfigure PSconfig .\PS.seek 1ml P%#A d`xb %M'Rq +991 h 'Rq .tdelay 2 seconds +200 .\PRV.dial :air P%H  +113 h  .t\PS.seek 2.5ml P%[' +1597 h ' .tdelay 2 seconds +200 #\Parking diluted lysate in Mixing Coil .\PSV.dial :mixloop P%W +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%]  +75 h .t\PRV.dial :CBV P%^  +38 h P%L% h % .t\PS.seek 1.5ml P%_' +1091 h ' .tdelay 2 seconds +200 .\PSV.dial :waste1,avoiding: PBV P%P  +150 h  .t\PS.seek :empty P%l' +1598 h ' #tBeginning samplePassF C'Q& h &W .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR,avoiding: PBV P%Z  +37 h P%c% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%\\' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%f  +37 h  .t\PS.seek 0ml P%I' +1598 h ' .t\PSV.dial PSR P%d +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%e' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :PTV,via: :air P%i +226 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRV,avoiding: :puck P%m @ +75 h @ .t\PS.seek 0ml P%h' +2610 h ' .t\PSV.dial :air P%V  +75 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :PTV P%b +75 h .t\PS.seek 0ml P%6' +2610 h ' C'F& h &W #t\moving diluted lysate into CBV-AIV line .\PSV.dial :air P%"  +75 h  .t\PS.seek 2.5ml P%r' +2610 h ' .tdelay 2 seconds +200 .\PSV.dial :mixloop P%C +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%j  +75 h .t\PRV.dial CBV P%x% h % .t\PS.seek 1.8ml P%p' +788 h ' .tdelay 3 seconds +300 .\PRV.dial :waste2 P%O @ +113 h @ .t\PS.seek :empty P%t' +1900 h ' #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%A  +75 h  .tdelay 2 seconds +200 P%`% h % .t\PS.seek 1.499ml P%T' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%?' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%|' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%u +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%;  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %a'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%y% h %r .t\PS.seek 0.475ml P%K' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%}  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +76 h  .t\PS.seek 2.5ml P%{' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%z +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%w  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %v'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%~  +37 h  .t\PS.seek 2.5ml P%(' +2610 h ' .t\PSV.dial :PML P%k +38 h .t\PS.seek 0ml P%Y' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 's& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*/ @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%$ +22 h  ."MFBPCR"delay 20 seconds +91 h P%1% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%!' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*, I +128 h I F*4% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b **'6 +66 .t\PSV.dial :waste1 P%0  +38 h  .t\PS.seek 0ml P%7' +1597 h ' .t\PSV.dial PSR P%) +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%8 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%3' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%' +21 h  F*2% h %6 ."MFBPCR"\AS.seek 0.329ml F*B'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%<' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%D' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*\  +22 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *:' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%= +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%#' +217 ."MFBPCR"\ATV.dial :AIV F*J I +43 h I F*N% h % ."MFBPCR"\AS.seek 0.469ml F*9' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%+' +2610 h ' .t\PSV.dial PSR P% +113 h P%%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%>' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%G  +37 h  .t\PS.seek 0ml P%R' +740 h ' ."MFBPCR"delay 5 seconds +500 F*U9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*g n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*S% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%H +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%M' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%W +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%]' +2610 h ' .t\PSV.dial PSR P%^ +113 h P%L% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%P  +38 h  .t\PS.seek 0ml P%_' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%Q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%l +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %f'k +2678 h 'k .t\PSV.dial :waste1 P%\\  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %d' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*I I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *i'V +850 h ' .t\PSV.dial :air P%m  +113 h  .t\PS.seek 2.5ml P%e' +2609 h ' .t\PSV.dial :mixloop P%V +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %h' +5107 h ' .t\PSV.dial :air P%b  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %o' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*F  +43 h  F*"% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *6'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*C I +43 h I F*j% h %Q ."MFBPCR"\AS.seek 0.01ml F*x' +171 h ' .t\PSV.dial :mixloop P%r +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %O' +618 h ' ."MFBPCR"\ATV.dial :waste F*p n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +85 h  F*A% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *`' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*t  +43 h F*T% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'?  +38 h  C'u% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C';'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'|  '  +37 h  +113  .t\CBV.dial :waste1 C'y @ +38 h @ .t\CS.seek 0ml C'a'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*K% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F* % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*}'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'q' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'z'4 +2648 h '4 C'w& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&~& h &ӷ M&% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &((- +264 h (- M&s& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&$1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C',& h & M&4% h %\  A(0% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (!( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ()& h &  M&7& h &- M&8% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +165 h ( M&& h & A(2& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &'' +90 h ' A(& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(*' +77 h ' A(3& h &  M&& h & ."WCR"\Elbow.seek 2 M&B( +6 h ( M&&& h & A(\ & h &  ."WCR"\Forearm.seek 2 + 300 counts M& '8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*g  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *S' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)9+ +6 h + S)H% h % C'R& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'W  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +21 h F*g% h % ."MFBPCR"\AS.seek 0.23ml F*H' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h % ."MFBPCR"\AS.seek 0.255ml F*#'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +21 h F*!% h %X ."MFBPCR"\AS.seek 0.24ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*L n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*^  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *['5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*M @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*l'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Q'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\\  +43 h  #"MFBPCR"\Swish #1 of 2 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *I'\  +1031 h '\  F*c% h %\  ."MFBPCR"\AS.seek 0.5ml F*m'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*Z% h %(A ."MFBPCR"\AS.seek 0.6ml F*e'\  +1031 h '\  F*b% h %\  ."MFBPCR"\AS.seek 0.5ml F*h'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*F% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *d' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*V % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3472 h 'V ."MFBPCR"\SPE.coast F*C  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*j  +64 h  ."MFBPCR"\ARV.dial :carrier F*r  +11 h  F*6% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*x  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +22 h ."MFBPCR"delay 2 seconds +200 F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*`  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*u% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*;  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*X' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*y % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*}  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226038 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'w @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +94 h  C'~% h % ."WCR"\CSV.dial between air and CTV C'E ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C's  ."WCR"\CSV.dial CTV C'  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '{'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'$  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'1  +38 h  C'4% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C', @ +76 h @ +50 S)0% h % ."WCR"\CBV.dial between CSV and waste2b C'8  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'7 ` +56 h ` ."WCR"\CS.seek 7.288ml C'2' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '3'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '(' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'\  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Y  +37 h  C'&% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'<' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)'% h % ."WCR"\CBV.dial between CSV and waste2b C'J  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C':' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'%  '@  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'B'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'*  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'v' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'N& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&>% h %\  A(g% h %X A(G. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (R(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (W& h &( M&H& h &ӷ M&+% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &!( +143 h ( M&#& h & A(L& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &^' +89 h ' A(9& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(P'K +254 h 'K M&]& h & A([& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C ."WCR"\Elbow.hold M&_, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(l- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(\\' +26 h ' M&f% h %C A(Q& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&c'o +21 h 'o M&I& h & A(Z& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &m' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(b' +36 h ' M&e& h & M&F% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +89 h '\  ."WCR"\Elbow.seek CC M&V(ӷ +143 h (ӷ M&d& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.seek CC M&j' +91 h ' ."WCR"Hand.close M&r/ +150 h / ."WCR"\CC.seek :open C'6( +416 h ( A(i& h &( A("& h &( M&S& h &ӷ M&p% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &x( +142 h ( M&t& h & A(T& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &A'C +93 h 'C ."WCR"\Elbow.hold M&`, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &u1 +150 h 1 M&% h %C A(;& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&|'o +20 h 'o M&?& h & A(y& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +23 h ' A(X% h % ."downElevator"\SE.seek :down A(K'X +100 (% h %B M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'a& h & .tHand.open M& 1 + A(6 + h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&'η +90 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'.& h &W .tHand.open M&~1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C's% h % .t\CSV.dial CSR C'  +37 h  C'$% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'{'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'k% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '1'[ +463 h '[ .t\CSV.dial :waste C'4 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C',% h %4 .t\CSV.dial :flush C'0  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'7% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C'2 @ +76 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'3& h &W C'\ % h %4 .t\CSV.dial :flush C'Y  +75 h  .t\CS.seek 5ml C'&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +38 h @ C'<% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  +37 h  .t\CBV.dial :waste2b C'J  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C' & h &W C'=% h %4 .t\CSV.dial :flush C'@  +75 h  .t\CS.seek 5ml C'%'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C': @ +38 h @ C'*% h %p .t\CS.seek 7ml C'B'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N '  +38 h +75 .t\CTV.dial :air C'>  +150 h .t\CS.seek 0ml C'g'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'H'4 +506 h '4 C'+& h &W C'#% h %4 .t\CSV.dial :flush C'L  +38 h  .t\CS.seek 3ml C'9'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +38 h @ C'U% h %0 .t\CS.seek 5ml C']'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'['4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +507 h '0 .t\CSV.dial :waste C'f +75 h .t\CS.seek 0ml C'Q'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'I& h &W C'Z% h %4 .t\CSV.dial CSR C'e  +150 h  C'F% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'^'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'P +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'd' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'C  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'c'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'\\  +112 h  C'm% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  '  +37 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C'"'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'S  +37 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 't'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +37 h  .t\CBV.dial :waste2b C'V  +76 h  .t\CS.seek 0ml C'u'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C';% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '?'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'y' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X '  +38 h +75 .t\CTV.dial :air C'x  +151 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'a +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +38 h @ C'n% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} +113 h .t\CTV.dial :air .\CS.seek 0ml C'K'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'R'0 +506 h '0 .t\CSV.dial :waste C'` +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +37 h @ C'z% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'6'0 +506 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C'~'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'5% h %p .t\CS.seek 7ml C'v'i +416 h 'i ."connect"\CSV.dial CTV C'k  .t\CTV.dial CSV C'{  +37 h  +113  .t\CBV.dial :waste1 C'4 @ +38 h @ .t\CS.seek 0ml C'1'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C',% h %4 .t\CSV.dial :flush C'0  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'7% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'2'4 +766 h '4 #tDisinfecting external Intake with kill C')& h &W C'3% h %4 .t\CSV.dial CSR C'\  +37 h  C'Y% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'(' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'< '&  +38 h +75 .t\CTV.dial :intake C''  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'J' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'=  +113 h  C'@% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C':' +1372 h ' .t\CSV.dial CBV C'* +113 h .t\CS.seek 0ml C'%'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'G( +416 h ( M&+& h &ӷ M&#% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +265 h (- M&D& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &9'η +91 h 'η .tHand.open M&]1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'l& h & .tHand.open M&I1 +2 h 1 M&Z& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &e(- +6 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&P/ +150 h / M&& h &- M&!% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'h& h &W .tHand.open M&1 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'S& h &W C'p% h %4 .t\CSV.dial CSR C'"  +113 h  C't% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'j'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'V% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'T'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ';'4 +2010 h '4 #t\Purging external Intake C'u& h &W C'?% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'x +76 h .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'a  +76 h #t\Flushing upper manifold C' & h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'A'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV C'| .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'K +38 h .t\CS.seek 0ml C'`'4 +506 h '4 C'R& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'w% h %0 .t\CS.seek 5ml C'z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'.'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&6& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'5( +415 h ( M&& h &ӷ M&k% h % .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +265 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &,'η +91 h 'η .tHand.open M&01 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [phasewithdil] begun 01:00:26 #\Preparing to sleep until 14:00 29-sep-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00 29-sep-18 #\Continuing at 14:00CEST29-Sep-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeployv2dil.rb:117\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:15:55, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST29-Sep-18" +263637 !"PCR"o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h25ml.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *)'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*\ % h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*=% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *@'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +31 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*+% h % ."MFBPCR"\AS.seek 0.81ml F*#'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*U'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*]% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*L  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*I' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h25ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*m% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*i  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *"'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*t  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*c% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*V  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*T  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*?'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*A'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*n % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h25ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*w'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*s  +65 h  F*$% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*3  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0108h25ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ARV.dial :carrier F*@  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*W  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*+ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*D I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*N' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*] % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.536ml F*L'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*l% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.6ml F*Q'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*H  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*f % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0108h25ml.pcr F*e% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*C  +21 h ."MFBPCR"\ATV.dial :air3 F*h % +87 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*t'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*V'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*T'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*n'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +21 h % ."MFBPCR"delay 3 seconds +300 F*}% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0111h25ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ARV.dial :carrier F*6  +10 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*{ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*1' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*7 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +21 h n ."MFBPCR"delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"User Abort!!:@checkpoint: omitted F*'' \\ #t-> exit .\AS.stop #m\EXIT @1537978586.01CEST @1538296868.31CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tundeployv2" .GoodNews.suppressMail "Starting 18tundeployv2 w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:41:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployv2 begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:41:28, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ S)&\ P% 0\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:41:33, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:41:39CEST30-Sep-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:41:33, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %8 .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C',(W +416 h (W .tHand.open M&+1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)%+ +6 h + S)'% h % C'/& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*I  +11 h  ."readyMFB"\ASV.dial :waste1 F*H  h +912 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'K% h %4 S)J% h % S)M% h % .tIntake.select :close S)0 +6 )N% h % .t\SSV.dial :exhaust S)O  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9Td +100 C'99 h 9Xd +100 C'I9 h 9(\\d +100 C'H9 h 9@`d +100 C'K9 h 9Wdd +100 C'J9 h 9nhd +100 C'M9 h 9ld +100 C'N9 h 9pd +100 C'O9 h 9td +100 C'G9 h 9xd +100 C'R9 h 9|d +100 C'Q9 h 9d +100 C'U9 h 9d +100 C'P9 h 9'd +100 C'l9 h 9>d +100 C'W9 h 9Td +100 C'S9 h 9kd +100 C'X9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'\\9 h 9d +100 C'^9 h 9 d +100 C'L9 h 9 d +100 C'V9 h 96d +100 C'_9 h 9Ld +100 C'b9 h 9bd +100 C'c9 h 9xd +100 C'Z9 h 9d +100 C'a9 h 9d +100 C'f9 h 9d +100 C'g9 h 9d +100 C'd9 h 9d +100 C'e9 h 9d +100 C'i9 h 9d +100 C'k9 h 9(d +100 C'm9 h 9>d +100 C'h9 h 9Td +100 C'p9 h 9id +100 C'`9 h 9d +100 C'o9 h 9d +100 C'j9 h 9d +100 C'r9 h 9d +100 C'C9 h 9d +100 C'?9 h 9d +100 C'u9 h 9  d +100 C'q9 h 9 d +100 C';9 h 9 *d +100 C'x9 h 9 ?d +100 C'y9 h 9 Td +100 C'{9 h 9 j d +100 C't9 h 9 $d +100 C'n9 h 9 (d +100 C'v9 h 9 ,d +100 C'~9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 & M&9& h &( &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(I'Z .t\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&M' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'O( +23 h 'Z +393 ( A(G& h &( A(R& h &( M&Q& h &ӷ M&U% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +142 h ( M&W& h & A(S& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'C +93 h 'C .t\Elbow.hold M&T, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &[1 +150 h 1 M&Y% h %C A(]& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&\\'o +20 h 'o M&^& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +23 h ' A(_% h %Z ."downElevator"\SE.seek :down A(b'X +100 (c% h % M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'f @ +2 A(6 +17 h @ .t\CS.seek 10ml C'g' +7 h ' .t\CSV.dial between air and CTV C'd ` +18 h ` .t\AIV.dial :bypass C'e { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'i& h & .tHand.open M&k1 +2 h 1 M&m& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&j'η +91 h 'η .tHand.close M&r/ +150 h / M&C& h &- M&?% h %η .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +264 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C't& h &W .tHand.open M&n1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P%| +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'\  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%w +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%}' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'#( +416 h ( M&(& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&+1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'/& h & M&% h %\  A($% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &  M&*& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &5( +166 h ( M&0& h & A(7& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A()& h &  .t\SE.seek 19.71 Pucks below Top Plate A( ' +66 h ' A(&& h &  M&8& h & .t\Elbow.seek 2 M&=( +6 h ( M&3& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&.'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down M&B& A(:'X h & M&D% h %8 .t\Forearm.seek :retract M&E'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&F(ӷ +143 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &9' +91 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&J1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C',  +19 h  .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'R' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'Q'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%S +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%M  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%[  +151 h  .t\PS.seek :empty P%Y' +2609 h ' .t\CBV.dial :waste1 C'] @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'_  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'P ` +19 h ` .t\CSV.dial :air C'l @ +37 h @ .t\CS.seek 10ml C'c' +2648 h ' .t\CSV.dial CTV C'Z  +37 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'\\ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'f  +151 h C'I& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&d% h %\  A(e% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (k(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (m& h &( M&h& h &ӷ M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &`( +143 h ( M&o& h & A(b& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &A' +89 h ' A(6& h &( .t\SE.seek -0.29 Pucks below Top Plate A(T'\\ +264 h '\\ M&r& h & A(C& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &?'C +26 h 'C .t\Elbow.hold M&a, +6 h , .t\Elbow.reconfigure TightElbow A(j- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(;' +27 h ' M&x% h %C A(p& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&u'o +20 h 'o M&O& h & A(t& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(v' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +415 h ( A(& h &( A( & h &( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &w( +143 h ( M& & h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &}1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'o +20 h 'o M&& h & A(q& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A({% h % ."downElevator"\SE.seek :down A(('X +100 (% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%+' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%s +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%2  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P%4 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&71 +2 h 1 M&)& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&8& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&'/ +150 h / M&1& h &- M&B% h %η .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'.(W +416 h (W C' & h &W .tHand.open M&:1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%,% h % .t\PS.seek 1.499ml P%9' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%%' +1094 h ' .t\PSV.dial :air P%g  +38 h  .t\PS.seek 0ml P%G' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%R +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%U  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%M  +76 h .tdelay 2 seconds +200 P%H% h %r .t\PS.seek 0.475ml P%[' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%]'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +75 h .t\PTV.dial :PRV,avoiding: :puck P%l @ +76 h @ .t\PS.seek 0ml P%Q' +2609 h ' .t\PSV.dial :air P%Z  +75 h  .t\PS.seek 2.5ml P%c' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%f' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%e +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%i  +75 h P%m% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'V & +50 h & .t\AWV.select :waste C' +7 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %b'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'6 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %F' +1313 h ' .t\PSV.dial :air P%"  +37 h  .t\PS.seek 2.5ml P%r' +2610 h ' .t\PSV.dial :PML P%C +38 h .t\PS.seek 0ml P%j' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t  .t\AWV.select :air F*O @@T .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +129 h I F*T% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%|' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%a' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%y +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*q I +42 h I F*{% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*(% h % ."MFBPCR"\AS.seek 0.041ml F*k'% +336 h ' .t\PSV.dial PSR P%Y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%s' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P% +112 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%2' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%+' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%4 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%*' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %)'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %8' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*3 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%'  +112 h  .t\PS.seek 2.5ml P%1' +2610 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*@  +43 h  F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *D'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*\ I +43 h I F* % h %Q ."MFBPCR"\AS.seek 0.01ml F*:' +171 h ' .t\PSV.dial :mixloop P%= +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*>'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *,' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*9% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'%  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'U'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  'M  +37 h  +113  .t\CBV.dial :waste1 C'H @ +38 h @ .t\CS.seek 0ml C'S'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*[% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C']% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'P'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*l  +22 h F*_% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*Z'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*\\% h %& ."MFBPCR"\AS.seek 0.092ml F*c'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'I' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'e'4 +2647 h '4 C'i& h &W #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +7 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&6/ +150 h / .t\CC.seek :open C'b( +416 h ( M&"& h &ӷ M&F% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &t'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'`& h & M&T% h %\  A(% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (A( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (u& h &  M&|& h &- M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +166 h ( M& & h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &y' +90 h ' A( & h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(?' +77 h ' A(a& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&}'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&q% h %8 ."WCR"\Forearm.seek :retract M&{'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*~  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *(' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)z+ +6 h + S)Y% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteR F*9  +21 h  ."MFBPCR"\AS.seek 0.3ml F*,': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*%'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*R % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*U  +87 h F*G% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *M'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*H I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*W'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*[ n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*L  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *]% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *g' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *S% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *l' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*_% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *^' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*Q% h % ."MFBPCR"\AS.seek 0.279ml F*\\'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Z n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*f % +43 h % F*I% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *i'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*m I +21 h I F*V% h %v ."MFBPCR"\AS.seek 0.059ml F*o'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*6 % +21 h % F*5% h %H ."MFBPCR"\AS.seek 0.069ml F*E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*"% h % ."MFBPCR"\AS.seek 0.769ml F*F' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h % q ."MFBPCR"\AS.seek 0.794ml F*p' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*C I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *`' +4416 h ' ."MFBPCR"\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.seek :empty F*'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*t% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*j  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*; % +108 h % F*y% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*w n +108 h n ."MFBPCR"\ASV.dial :wasteR F*}% h %  ."MFBPCR"\AS.seek 0.09ml F*?' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*{' +625 h ' ."MFBPCR"\ATV.dial :air3 F*b % +21 h % F*c% h % ."MFBPCR"\AS.seek 0.13ml F*v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*~% h % ."MFBPCR"\AS.seek 0.23ml F*Y' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*A% h %X ."MFBPCR"\AS.seek 0.24ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *2'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*s @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*k I +85 h I ."MFBPCR"\AS.seek 0ml F*7'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*4'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *3'\  +1032 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*''(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F**% h %(A ."MFBPCR"\AS.seek 0.6ml F*1'\  +1031 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*@% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *8' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*B % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*\  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *>'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*,  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*%' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*U  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*X' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*H % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*]'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +22 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*Z  +65 h ."MFBPCR"\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226038 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'V  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'i @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'5 @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'.  +94 h  C'"% h % ."WCR"\CSV.dial between air and CTV C'E ` +19 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'x  'F  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'C  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'` @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'n  +95 h  C't% h % ."WCR"\CSV.dial between air and CTV C'| ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'a'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'j  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'; @ +76 h @ +50 S)y% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'} ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'b' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'c  +151 h C'{& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(~% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&Y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +143 h ( M&& h & A($& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(z& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +253 h 'K M&/& h & A(2& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &k'C +26 h 'C ."WCR"\Elbow.hold M&+, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(7- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&)% h %C A(4& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&0'o +20 h 'o M&3& h & A(*& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(<' +36 h ' M&1& h & M&@% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&B(ӷ +143 h (ӷ M&8& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&=/ +150 h / ."WCR"\CC.seek :open C'D( +416 h ( A(& h &( A(&& h &( M&(& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &:( +142 h ( M&& h & A(9& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C ."WCR"\Elbow.hold M&,, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &R1 +150 h 1 M&>% h %C A(U& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&G'o +20 h 'o M&%& h & A(H& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(X% h % ."downElevator"\SE.seek :down A(['X +100 (L% h %B M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&]1 +2 A(6 h 1 M&_& h & M&l% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&f/ +150 h / M&Q& h &- M&P% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +265 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &5' +91 h ' .t\CC.seek :closed C'o(W +415 h (W C'.& h &W .tHand.open M&"1 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'F& h &W C'x% h % .t\CSV.dial CSR C'h  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'C% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'T +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'`% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'% h %4 .t\CSV.dial :flush C'j  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV C'y  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'q  +76 h  .t\CS.seek 0ml C'w'4 +765 h '4 #t\Flushing upper manifold C'}& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'?% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c '{  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'Y'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'$  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C') +75 h .t\CS.seek 0ml C'4'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'3& h &W C'*% h %4 .t\CSV.dial CSR C'1  +151 h  C'@% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'A' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +113 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'8' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'\  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '0'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'+'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'&'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'(  +37 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'B  +75 h  .t\CS.seek 0ml C'R'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'>  +38 h  C'U% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'N  +38 h +75 .t\CTV.dial :air C':  +151 h .t\CS.seek 0ml C'L'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +507 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing upper manifold C'_& h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV C'Z .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C', +38 h .t\CS.seek 0ml C'f'4 +506 h '4 C'Q& h &W C'P% h %4 .t\CSV.dial :flush C'W  +38 h  .t\CS.seek 3ml C'\\'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'e% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'm'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'F% h %4 .t\CSV.dial :flush C'x  +37 h  .t\CS.seek 5ml C'h'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'5% h %p .t\CS.seek 7ml C'b'i +416 h 'i ."connect"\CSV.dial CTV C'C  .t\CTV.dial CSV C'd  +38 h  +113  .t\CBV.dial :waste1 C'T @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'`% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'|% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'u& h &W C'a% h %4 .t\CSV.dial CSR C'  +37 h  C'j% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +37 h +76 .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'q' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C';  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +607 h '4 .t\CSV.dial CSR C'  +112 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1373 h ' .t\CSV.dial CBV C'? +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &c(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&/1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&31 +2 h 1 M&*& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &1(- +7 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&!/ +150 h / M&k& h &- M&A% h %η .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C'+(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'J% h %4 .t\CSV.dial CSR C'&  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'B% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '9'[ +463 h '[ .t\CSV.dial :waste C'> +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +2010 h '4 #t\Purging external Intake C'R& h &W C'%% h %4 .t\CSV.dial :flush C'N  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C': +75 h .t\CTV.dial :intake C'H  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'L'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'M& h &W C'_% h %4 .t\CSV.dial :flush C'l  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'<% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'G .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'Z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +506 h '0 .t\CSV.dial :waste C'[ +38 h .t\CS.seek 0ml C','4 +506 h '4 C'& h &W C'Q% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'i% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'm +75 h .t\CS.seek 0ml C'.'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&D& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &E(ӷ +6 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'5( +416 h ( M&h& h &ӷ M&C% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +265 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +90 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Preparing to sleep until 14:00 2-oct-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00 2-oct-18 #\Continuing at 14:00CEST02-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:110\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:43:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST02-Oct-18" +263638 !"PCR"o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h1500ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *u'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*V% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*1'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *&'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*B  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*>' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*9  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +108 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*:'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +22 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*l' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h1500ml.pcr F*Z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*^  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*D'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*`% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0108h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*6% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*c' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*/ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*2  +107 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +22 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0108h1500ml.pcr F*1% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*\  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*B'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*>  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +31 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0111h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*D  +10 h  F*8% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *b'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*d I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*| n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*u n +21 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0111h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*!  +32 h  ."MFBPCR"\ATV.dial :air3 F*4 % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*9  +11 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*l I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*s % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*^  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +22 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +22 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*x @@T *=  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*+  +151 h F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +423 h ' ."MFBPCR"\ATV.dial :ASV F*E n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*"% h % ."MFBPCR"\AS.seek 0.62ml F*d'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *|'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*I'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *n' +424 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +21 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*u' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*j' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*; n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*q% h % zG ."MFBPCR"\AS.seek 0.365ml F*y' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*{% h % ."MFBPCR"\AS.seek 0.579ml F*w' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*6% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*o'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*c % +108 h % F*~% h %V ."MFBPCR"\AS.seek 0.01ml F*2' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*$% h % ."MFBPCR"\AS.seek 0.58ml F**' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h % q ."MFBPCR"\AS.seek 0.605ml F*!'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*A% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*k  +21 h  ."MFBPCR"\AS.seek 0ml F*Y'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *1'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*'  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *\ ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*v n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*J  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*( n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*0  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*R  +42 h  F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *9': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*U  +64 h  F*>% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *%'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*X n +65 h n F*H% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *:' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*S I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*M  +43 h  F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *G': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*_  +64 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *Z'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*< n +65 h n F*^% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*Q  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*f n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *P'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*, n +150 h n ."MFBPCR"\AS.seek 0ml F*e'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*i  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*W % +43 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*m% h % ."MFBPCR"\AS.seek 0.96ml F*D'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*C% h % ."MFBPCR"\AS.seek 0.97ml F*h'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*E I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *"'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *`'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *b'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*|  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *I' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F*n' +9880 h ' ."MFBPCR"\ATV.dial :waste F*p n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*a% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*u % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *V'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*o% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*~% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*4  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*J n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*('V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1538481600.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )0 h  C' '& h  P% % h  F* *  h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)R% h % .t\SS.configure SSconfig F*#,,'T(^20( b C'N& S)#.0X% h % .t\PS.configure PSconfig M&9% P%#A d`xb h %\  .t\Forearm.configure LooseForearm M&#Q@2^d^2b &X& h &- .t\Elbow.configure LooseElbow A(H& M&$ &.,(#b h &( .t\SC.configure SCloose A($.(\ 1~b (%% h %X .t\SE.configure SEconfig .\CH.coast A(#QK-0-\ b C'S  h  .t\CH.configure CHconfig .\PH.coast C'\ 2 P%:  h  .t\PH.configure PHconfig P%\ 2 +29 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +6 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'L% h %p P%M% h %q ."rdySampler"Intake.select :close ."rdyProcessing"\PSV.dial :air M&l% ."rdySampler"\Exhaust.select :close P%]  ."rdySampler"\CTV.dial :air h %= C'_  M&s% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &G'\ h ."rdySampler"\CSV.dial :waste1 C'< h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)^ h  S)Z  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close .t\Elbow.jog 1500 M&/ &Q* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b '['4 ."rdySampler"\SS.home.seek 0ml S)#.0X 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:44:18CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'C& h & .tHand.open M&1 +150 h 1 M&E& h &M M&h% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &`'η +91 h 'η .tHand.close M&d/ +150 h / M&|& h &- M&% h %η .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'a(W +416 h (W C'n& h &W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'K% h %4 .t\CSV.dial CSR C'j  +150 h  C';% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'q +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'r& h &W C'y% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '{' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} '  h +75 .t\CTV.dial :intake C'V  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'w  +75 h #t\Flushing upper manifold C'& h &W C'6% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'o% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'c .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C'7 +37 h .t\CS.seek 0ml C'$'4 +507 h '4 C'& h &W C'@% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C'2'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'*% h %0 .t\CS.seek 5ml C'A'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'!'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'z'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'J( +416 h ( M&v& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &T(- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&.1 +150 h 1 M&#% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:44:24CEST30-Sep-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:44:18CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*t% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'x& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&0% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(&% h %X A(. h ., A( & h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&R% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &N( +6 F*B% h %&K ."readyMFB"\ATV.dial :waste2 F*U % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *>' +160 h ( M&9& h & A(X& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +89 h ' A(%& h &  .t\SE.seek 17.71 Pucks below Top Plate A(S'* +87 h '* A(:& h &  M&L& h & .t\Elbow.seek 2 M&M( +6 h ( M&l& h & A(s& h &  .t\Forearm.seek 2 + 300 counts M&_'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &9 h 9d +100 C'`9 h 9'd +100 C'O9 h 9>d +100 C'9 h 9Td +100 C'b9 h 9kd +100 C'+9 h 9d +100 C'n9 h 9d +100 C' 9 h 9d +100 C'|9 h 9d +100 C'h9 h 9d +100 C'9 h 9d +100 C'K9 h 9 d +100 C'j9 h 9 d +100 C'I9 h 96d +100 C'p9 h 9Ld +100 C'm9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'q9 h 9d +100 C';9 h 9d +100 C' 9 h 9d +100 C'y9 h 9d +100 C'?9 h 9d +100 C'9 h 9d +100 C'r9 h 9(d +100 C'V9 h 9>d +100 C'9 h 9Td +100 C'}9 h 9id +100 C'9 h 9d +100 C'{9 h 9d +100 C'9 h 9d +100 C'69 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'w9 h 9  d +100 C'o9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'c9 h 9 j d +100 C'79 h 9 $d +100 C'/9 h 9 (d +100 C'9 h 9 ,d +100 C'@9 h 9 0d +100 C'$9 h 9 4d +100 C'49 h 9 8d +100 C'*9 h 9 , +6 h , .t\Elbow.reconfigure TightElbow A(`- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(O' +27 h ' M&% h %C A(b& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&+'o +20 h 'o M&n& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &|' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(h' +37 h ' M&& h & M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +89 h '\  .t\Elbow.seek CC M&I(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'q( +415 h ( A(;& h &( A( & h &( M&y& h &ӷ M&?% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &r( +143 h ( M&V& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &}'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &{1 +150 h 1 M&% h %C A(6& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&& h & A(w& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &o' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (% h %1 M&c% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'/ @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'@ ` +19 h ` .t\AIV.dial :bypass C'$ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'4& h & .tHand.open M&*1 +2 h 1 M&2& h & M&A% h %\  .t\Elbow.seek FlushPuck::Garage M&~(- +166 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&Y'η +91 h 'η .tHand.close M&3/ +150 h / M&1& h &- M&\ % h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'-(W +416 h (W C'a& h &W .tHand.open M&)1 +150 h 1 M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%t  +38 h  .t\PS.seek 0ml P%#' +1597 h ' .t\PSV.dial PSR P%0 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%x' +2610 h ' ."connect"\PRV.dial CBV P%&  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%B  +37 h  .t\PS.seek 2.5ml P%R' +2610 h ' .t\PSV.dial :mixloop P%9 +38 h .t\PS.seek 0ml P%X' +2609 h ' .t\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%%' +2610 h ' .t\PSV.dial :mixloop P%L +37 h .t\PS.seek 0ml P%:' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.seek CC M&^' +90 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C's( +416 h ( M&N& h &ӷ M&H% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &P(- +265 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &F'η +91 h 'η .tHand.open M&M1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'J& h & M&=% h %\  A(G% h %X A(W. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (e( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (\\& h &  M&i& h &- M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &"( +166 h ( M&& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(& h &  .t\SE.seek 16.71 Pucks below Top Plate A(d'; +96 h '; A(& h &  M&`& h & .t\Elbow.seek 2 M&( +6 h ( M&b& h & A(n& h &  .t\Forearm.seek 2 + 300 counts M& '8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&K& A(C'X h & M&f% h %8 .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'O(W +416 h (W .tHand.open M&|1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'j  +150 h  .t\CSV.dial CTV C'8  +19 h  .t\CS.seek 5ml C';'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C' ' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'y'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%?  +37 h  .t\PS.seek 2.5ml P%V' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%I +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%{  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C'6 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'w @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'r @ +37 h @ .t\CS.seek 10ml C'' +2648 h ' .t\CSV.dial CTV C'c  +37 h  .t\CS.seek 0ml C'/'4 +2647 h '4 .t\CBV.dial :waste1 C'} @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'@  +151 h C'$& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&4% h %\  A(*% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (A(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&h& h &ӷ M&o% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +143 h ( M&& h & A(E& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &( .t\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') M&3& h & A(1& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &\ 'C +25 h 'C .t\Elbow.hold M&7, +7 h , .t\Elbow.reconfigure TightElbow A(Y- h -+) M&$&.,(\ #b .t\SE.seek 3.04 Pucks below Top Plate A(u' +27 h ' M&v% h %C A(~& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M& 'o +21 h 'o M&q& h & A(a& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &)' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(!' +36 h ' M&& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&t(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h &( A(& h &( M&x& h &ӷ M&B% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &9( +142 h ( M&R& h & A(U& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'C +93 h 'C .t\Elbow.hold M&L, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &%1 +150 h 1 M&T% h %C A(5& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &<'o +21 h 'o M&Z& h & A('& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &(' +22 h ' A(-% h % ."downElevator"\SE.seek :down A(N'X +100 (H% h %  M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%S  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%M' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%l +38 h .t\PRV.dial :air P%Q  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%J  +38 h .t\CBV.dial AIV C'=  +38 h .t\PRV.dial between CBV and air P%G  +18 h  .t\PRV.dial :air P%W  +19 h  P%\\% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %i' +251 h ' .t\PRV.dial :waste2 P%z @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %F' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&& h & M&P% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&]/ +150 h / M&& h &- M&K% h %η .t\Forearm.seek :retract M&f'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +265 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +91 h ' .t\CC.seek :closed C'd(W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  C's& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%| @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%>  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%j  +76 h  .tdelay 2 seconds +200 P%8% h % .t\PS.seek 1.499ml P%+' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%e' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%;' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%?  +37 h  .tdelay 2 seconds +200 P%:% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%I% h %r .t\PS.seek 0.475ml P%{' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%V  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%6'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%w  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%r @ +75 h @ .t\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial :air P%c  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%} +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%@' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%$  +75 h  .t\PS.seek 2.5ml P%4' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%* +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%2  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %h' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'o & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %E'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1312 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :PML P%1 +37 h .t\PS.seek 0ml P%Y' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'v& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'~& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*a  .t\AWV.select :air F*q @@T .t\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%.% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*k I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *\ '6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%0' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%u' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%) +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%7' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*&  +22 h ' .t\PSV.dial PSR P% +21 h  F*O% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%x' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*B % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +38 h  .t\PS.seek 0ml P%#' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*t  +22 h  F*%% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *T' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%5 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Z' +217 ."MFBPCR"\ATV.dial :AIV F*' I +43 h I F*-% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%9 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%H' +2610 h ' .t\PSV.dial PSR P%^ +113 h P%X% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%S' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*( n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*A'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%l' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%Q +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%J' +2609 h ' .t\PSV.dial PSR P%= +113 h P%G% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%W' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\\  +37 h  .t\PS.seek 0ml P%M' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%z +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%i' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%P  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %`' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*b I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *L'V +849 h ' .t\PSV.dial :air P%]  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%K +38 h .t\PS.reconfigure PSslow P%#A 2`xb .t\PS.seek 0ml P%_' +5106 h ' .t\PSV.dial :air P%n  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %,' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*p  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *f'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F* % h %Q ."MFBPCR"\AS.seek 0.01ml F*' +170 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %s' +618 h ' ."MFBPCR"\ATV.dial :waste F*| n +129 h n ."MFBPCR"\AS.seek :empty F*m'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*>  +86 h  F*j% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *8' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*F  +43 h F*+% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'e  +37 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'?'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C':' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C';  '  +38 h  +113  .t\CBV.dial :waste1 C'I @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*V  +22 h ."MFBPCR"delay 3 seconds +300 F*{% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'w  +37 h  C'6% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*r  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*c'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*y n +107 h n F*}% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'$' +1625 h ' ."connect"\CSV.dial CTV C'@  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'*'4 +2647 h '4 C'2& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'E( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &3(- +264 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &a'η +91 h 'η .tHand.open M&1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'k& h & M&% h %\  A(% h %X A([. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&0& h &- M&)% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &u( +166 h ( M&&& h & A(O& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  ."WCR"\SE.seek 15.71 Pucks below Top Plate A(\ 'L +107 h 'L A(7& h &  M&B& h & ."WCR"\Elbow.seek 2 M& ( +7 h ( M&U& h & A(t& h &  ."WCR"\Forearm.seek 2 + 300 counts M&%'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &x/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down M&R& A(5'X h & M&'% h %8 ."WCR"\Forearm.seek :retract M&-'\ +91 h '\  ."WCR"\Elbow.seek CC M&#(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&T& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +64 ."MFBPCR"\ATV.dial :waste2 F*^ % +27 h ' ."WCR"\CC.seek :closed C'X(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +400 h (W ."WCR"Hand.open M&!1 +150 h 1 M&<% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*(  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'S& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'Q  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +64 h  F*j% h % ."MFBPCR"\AS.seek 0.52ml F*m' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*F n +65 h n ."MFBPCR"\ASV.dial :wasteR F*+  +21 h  ."MFBPCR"\AS.seek 0.3ml F*8': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*e'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*?  +87 h F*;% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *'N +1026 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*I I +43 h I F*:% h %N ."MFBPCR"\AS.seek 0.109ml F*V'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*{ n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*w  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *6% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3301 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *r' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*y% h % ."MFBPCR"\AS.seek 0.279ml F*}'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*c n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*@ % +43 h % F*$% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *2'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +22 h I F*o% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*"% h %H ."MFBPCR"\AS.seek 0.069ml F*g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +21 h % ."MFBPCR"delay 3 seconds +300 F*v% h % q ."MFBPCR"\AS.seek 0.794ml F*~' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*.'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*1 I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *k' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*[ % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*a% h % ."MFBPCR"\AS.seek 0.579ml F*&' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*3'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*B% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*U' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Y  +43 h  ."MFBPCR"\AS.seek 0ml F*x'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *''V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*u % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*T' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*9 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*%% h %  ."MFBPCR"\AS.seek 0.09ml F*\ ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +85 h I F*5% h % ."MFBPCR"\AS.seek 0.12ml F*-' +626 h ' ."MFBPCR"\ATV.dial :air3 F*E % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*(% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h % ."MFBPCR"\AS.seek 0.255ml F*Z'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*G n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*=  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *W'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*l @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*A I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*P  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *b'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*]'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*i% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*n% h %\  ."MFBPCR"\AS.seek 0.5ml F*_'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*p% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *`' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*K % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0215h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*C  +10 h  F*f% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*8  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*e' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*?  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*I % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*6'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*V n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*c  +65 h ."MFBPCR"\ARV.dial :air1 F*}  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226068 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'o  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'2 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'" @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'd  +95 h  C'% h % ."WCR"\CSV.dial between air and CTV C'g ` +18 h ` ."WCR"\CS.seek 7.288ml C'h' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'v  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '~' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'1  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'.  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'[' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'k @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C')  +94 h  C'a% h % ."WCR"\CSV.dial between air and CTV C'0 ` +56 h ` ."WCR"\CS.seek 7.288ml C'O' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '&  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '7'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'B  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '3' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C't  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Y  +38 h  C'U% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'x' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'R' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'u @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C''  +94 h  C'9% h % ."WCR"\CSV.dial between air and CTV C'% ` +57 h ` ."WCR"\CS.seek 7.288ml C'T' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '^  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '5'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'E' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'-& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&<% h %\  A((% h %X A(!. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (S(( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Q& h &( M&& h &ӷ M&H% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&Z& h & A(G& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &=' +89 h ' A(& h &( ."WCR"\SE.seek 3.71 Pucks below Top Plate A(\\' +224 h ' M&J& h & A(W& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &A'C +26 h 'C ."WCR"\Elbow.hold M&M, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A( - h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(P' +27 h ' M&% h %C A(z& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'o +20 h 'o M&b& h & A(i& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &]' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(n' +37 h ' M&& h & M&p% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  ."WCR"\Elbow.seek CC M&K(ӷ +142 h (ӷ M&`& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M& ' +91 h ' ."WCR"Hand.close M&C/ +150 h / ."WCR"\CC.seek :open C'f( +416 h ( A(L& h &( A(& h &( M&N& h &ӷ M&|% h % ."WCR"\Forearm.seek :retract M&>'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&F& h & A(+& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &j'C +92 h 'C ."WCR"\Elbow.hold M&8, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&m% h %C A(?& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&;'o +21 h 'o M&e& h & A(I& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &:' +22 h ' A(/% h % ."downElevator"\SE.seek :down A({'X +100 (w% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +90 h '\  +21 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&61 +2 A(6 h 1 M&& h & M&r% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.seek :garage M&}'η +91 h 'η .tHand.close M&@/ +150 h / M&y& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +265 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'd& h &W .tHand.open M&1 +150 h 1 M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'v% h % .t\CSV.dial CSR C'h  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'4'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '1% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '.'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'[& h &W C'k% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ')'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'0% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  '  +37 h  +113  .t\CBV.dial :waste1 C'O @ +76 h @ .t\CS.seek 0ml C'B'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'7& h &W C't% h %4 .t\CSV.dial :flush C'Y  +76 h  .t\CS.seek 5ml C'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +38 h @ C'x% h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C''  +76 h  .t\CS.seek 0ml C'9'4 +766 h '4 #t\Flushing upper manifold C'%& h &W C'R% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'^'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'\ % h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '-  +37 h +76 .t\CTV.dial :air C'<  +150 h .t\CS.seek 0ml C'('4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C'Q +37 h .t\CS.seek 0ml C''4 +507 h '4 C'H& h &W C'Z% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +38 h @ C'% h %0 .t\CS.seek 5ml C'J'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'W'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'z'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'b& h &W C'i% h %4 .t\CSV.dial CSR C'  +151 h  C'p% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'='L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ +113 h .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'`' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'P  +113 h  C']% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'M'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  'X  +37 h  +113  .t\CBV.dial :waste1 C'L @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'N  +37 h  C'|% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '+' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'K  +75 h  .t\CS.seek 0ml C' '4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'm  +38 h  C'?% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CBV C'/ .t\CBV.dial CSV C'>  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'w'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'6'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'r% h %4 .t\CSV.dial :flush C'j  +38 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +38 h @ C'% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CBV C': .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'{'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'S'0 +506 h '0 .t\CSV.dial :waste C'8 +38 h .t\CS.seek 0ml C'@'4 +506 h '4 C'y& h &W C'% h %4 .t\CSV.dial :flush C'V  +38 h  .t\CS.seek 3ml C'}'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +37 h @ C'*% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C'd +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'g& h &W C'% h %4 .t\CSV.dial :flush C'v  +37 h  .t\CS.seek 5ml C'h'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'"% h %p .t\CS.seek 7ml C'E'i +416 h 'i ."connect"\CSV.dial CTV C'1  .t\CTV.dial CSV C'4  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'.'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'[& h &W C'k% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'~'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'0% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'O'4 +766 h '4 #tDisinfecting external Intake with kill C' & h &W C'7% h %4 .t\CSV.dial CSR C't  +37 h  C'Y% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'B'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'3' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'x 'U  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C''' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'u  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '%'4 +607 h '4 .t\CSV.dial CSR C'R  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'9'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'T' +1373 h ' .t\CSV.dial CBV C'\ +113 h .t\CS.seek 0ml C'^'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&-& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&(' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'!( +416 h ( M&H& h &ӷ M&Z% h % .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&J1 +150 h 1 M&W% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&b1 +2 h 1 M&i& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.seek :garage M&='η +91 h 'η .tHand.close M&\\/ +150 h / M&A& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +264 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'_& h &W .tHand.open M&X1 +150 h 1 M&L% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'N& h &W C'|% h %4 .t\CSV.dial CSR C'  +113 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'K% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '+'[ +463 h '[ .t\CSV.dial :waste C'm +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '?'4 +2010 h '4 #t\Purging external Intake C' & h &W C'e% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.reconfigure CSfast C'#d @b .t\CS.seek 10ml C'/' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'I  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'w'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'r  +75 h  .t\CS.seek 5ml C'j'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'n% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +113 h .t\CTV.dial :air .\CS.seek 0ml C':'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +506 h '0 .t\CSV.dial :waste C'{ +38 h .t\CS.seek 0ml C'8'4 +506 h '4 C'S& h &W C'y% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'V'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'2% h %0 .t\CS.seek 5ml C'*'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'}'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'd'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &g(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'"( +416 h ( M&h& h &ӷ M&1% h % .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +90 h '\  #t\Completed [coreNEG] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST03-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:46:05CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST03-Oct-18" +263667 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0215h.pcr F*0% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*&  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.51ml F* 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*U'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +64 h  F*R% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +32 h  ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*o% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*G  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0217h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +11 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*L  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*N  +21 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*F  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*^' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*K  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*m' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +107 h  ."MFBPCR"delay 7 seconds +700 F*?% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*I n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*r' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*n% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0217h.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*c  +22 h ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*2'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*v  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *`'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*[  +31 h  ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*)% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*7  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0220h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*%% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *-'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*H n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*(' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*# I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*J % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*G'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*W  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*z'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*P'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0220h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *N'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +21 h F*X% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*^% h %n ."MFBPCR"\AS.seek 0.82ml F*+'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*m  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*j'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*S% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0222h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*f  +11 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*4 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*0 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*q' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*7  +108 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*'% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CEST1538519392.11 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0222h.pcr F*R% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*T  +21 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*-% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*('q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*J  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*P'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*L'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0300h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*+  +10 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*w  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*r I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*;% h %Fj ."MFBPCR"\AS.seek 0.524ml F*n' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*l % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*c  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*j'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +21 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*@ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*V'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*}  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*f  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*` % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0300h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*v @@T *C  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +150 h F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *[' +424 h ' ."MFBPCR"\ATV.dial :ASV F*g n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*4'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*k n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *0'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *)' +423 h ' ."MFBPCR"\ATV.dial :air3 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*&% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h % H ."MFBPCR"\AS.seek 0.735ml F*Y' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*B% h % Ű ."MFBPCR"\AS.seek 0.72ml F*U' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*u n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*'% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*R % +43 h % F*3% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*-% h % ."MFBPCR"\AS.seek 0.579ml F*9' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %  ."MFBPCR"\AS.seek 0.604ml F*Q'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*5' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*# n +86 h n ."MFBPCR"\ASV.dial :wasteR F*Z  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*(% h %V ."MFBPCR"\AS.seek 0.01ml F*W' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*G% h % ."MFBPCR"\AS.seek 0.58ml F*i' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h % q ."MFBPCR"\AS.seek 0.605ml F*\\'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*= n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*A  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*]  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *P'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*! n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +202 h 'R ."MFBPCR"\SPE.coast F*|  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*N n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *F' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *+': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*?  +65 h  F*m% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *e'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*/ n +65 h n F*I% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *;': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*l% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *:'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*n n +64 h n F*c% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *S' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*y  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *j': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*@ n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*8 n +151 h n ."MFBPCR"\AS.seek 0ml F**'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*2  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*V % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *}' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*f'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*1% h % ."MFBPCR"\AS.seek 0.97ml F*h'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*g I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *k'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*0  +43 h  F*.% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *$' +9988 h ' ."MFBPCR"\ATV.dial AIV F*q I +43 h I ."MFBPCR"\AS.seek 0.02ml F*)' +9880 h ' ."MFBPCR"\ATV.dial :waste F*~ n +129 h n ."MFBPCR"\AS.seek 0ml F*&'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*7% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*t  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *o'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F*5 % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*(% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*W'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *P'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*L % +108 h % ."MFBPCR"\AS.seek 0ml F*N'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +3563006 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% %X h  F* *  h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *F  h  .t\SPE.configure SPEconfig F*\ 2 *^% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S) % h % .t\SS.configure SSconfig C'>& h & S)#.0X 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:47:04CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&1 +150 h 1 M&g& h &M M&h% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &k'η +91 h 'η .tHand.close M&4/ +150 h / M&0& h &- M&.% h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C'7(W +416 h (W C')& h &W .tHand.open M&&1 +150 h 1 M&t% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'x& h &W C'O% h %4 .t\CSV.dial CSR C'Y  +150 h  C'u% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'B'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'U% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +2009 h '4 #t\Purging external Intake C'3& h &W C'% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '-' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T '%  h +75 .t\CTV.dial :intake C'o  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'<'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'9  +75 h #t\Flushing upper manifold C'H& h &W C'% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C'Z'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +37 h @ C'% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'('4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C'& h &W C'p% h %4 .t\CSV.dial :flush C'b  +38 h  .t\CS.seek 3ml C'W'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +38 h @ C'i% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\\'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +507 h '0 .t\CSV.dial :waste C'A +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'|( +416 h ( M&!& h &ӷ M&L% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &"(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &N'η +91 h 'η .tHand.open M&D1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:47:10CEST30-Sep-18\\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:47:04CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*,% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'v& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(X. h ., A( & h &  .t\SC.reconfigure SCloose A($.(\ 1~b (F& h &  M&^& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &>( +6 F*K% h %&K ."readyMFB"\ATV.dial :waste2 F*? % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *m' +160 h ( M&+& h & A(/& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &I' +89 h ' A(e& h &  .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(& h &  M&w& h & .t\Elbow.seek 2 M&( +7 h ( M&r& h & A(l& h &  .t\Forearm.seek 2 + 300 counts M&'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &n/ +150 h / ."downElevator"\SE.seek :down M&6& A(c'X h & M&:% h %8 .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.seek CC M&8(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +90 h ' .t\CC.seek :closed C'`(W +416 h (W .tHand.open M&C1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)V+ +7 h + S)*% h % C'1& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +150 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S)I +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'T9 h 9kd +100 C'Q9 h 9d +100 C'<9 h 9d +100 C'9 h 9d +100 C'Z9 h 9d +100 C'9 h 9d +100 C'#9 h 9d +100 C'(9 h 9 d +100 C'R9 h 9 d +100 C'H9 h 96d +100 C'J9 h 9Ld +100 C'9 h 9bd +100 C'p9 h 9xd +100 C'59 h 9d +100 C'G9 h 9d +100 C'z9 h 9d +100 C'i9 h 9d +100 C'b9 h 9d +100 C'W9 h 9d +100 C'\\9 h 9d +100 C'A9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C']9 h 9d +100 C'=9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'79 h 9d +100 C'L9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'_9 h 9 ?d +100 C'a9 h 9 Td +100 C'D9 h 9 j d +100 C'!9 h 9 $d +100 C'9 h 9 (d +100 C'P9 h 9 ,d +100 C',9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'X9 h 9 9 h 9 d +100 C'I9 h 9 d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'y9 h 9 d +100 C'9 h 9 d +100 C'9 h 9 1d +100 C'9 h 9 Dd +100 C'S9 h 9 Xd +100 C'c9 h 9 kd +100 C'C9 h 9 ~d +100 C'|9 h 9 d +100 C'*9 h 9 d +100 C'19 h 9 d .t\CH.hold 5:00,85C C';   P h  +30000  C'29 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'}'4 +423 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'V @ +112 h @ .t\CSV.dial :air C'@ @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'[ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'0 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'.'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'm +113 h .t\CBV.dial CSV C'  +19 h C'% h %4 .t\CS.seek 5ml C'q'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'$  +38 h C'% h %p .t\CS.seek 7ml C'E'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'M @ +38 h @ .t\CS.seek 10ml C'k' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C't ` +131 h ` #tLysing Complete C'x& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&O% h %\  A(Y% h %X A(). h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (&(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (~& h &( M&4& h &ӷ M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &B( +142 h ( M&'& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &3' +90 h ' A(& h &( .t\SE.seek 4.71 Pucks below Top Plate A(\ ' +214 h ' M&u& h & A(%& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &o'C +25 h 'C .t\Elbow.hold M&9, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(-- h -+ .t\SE.seek 5.04 Pucks below Top Plate A(' +27 h ' M&% h %C A(T& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&Q'o +21 h 'o M&<& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &Z' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(' +36 h ' M&#& h & M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +90 h '\  .t\Elbow.seek CC M&H(ӷ +142 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&p' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C'G( +416 h ( A(z& h &( A(i& h &( M&b& h &ӷ M&W% h % .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &A( +142 h ( M&& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &]1 +150 h 1 M&=% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M& 'o +21 h 'o M&7& h & A(L& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(_'X +100 (a% h % M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'P' +6 h ' .t\CSV.dial between air and CTV C', ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&X1 +2 h 1 M& & h & M&F% h %\  .t\Elbow.seek FlushPuck::Garage M&s(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&?'η +91 h 'η .tHand.close M&v/ +150 h / M&/& h &- M&N% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' .t\CC.seek :closed C'd(W +416 h (W C'& h &W .tHand.open M&61 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%l +151 h P%:% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%S  h .t\PS.seek 0ml P%c' +2610 h ' .t\PSV.dial :air P%C  +37 h  .t\PS.seek 2.5ml P%|' +2610 h ' .t\PSV.dial :mixloop P%* +38 h .t\PS.seek 0ml P%1' +2609 h ' .t\PSV.dial :air P%;  +38 h  .t\PS.seek 2.5ml P%2' +2610 h ' .t\PSV.dial :mixloop P%g +37 h .t\PS.seek 0ml P%V' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&@& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'( +416 h ( M&m& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &.(- +265 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &q'η +91 h 'η .tHand.open M&M1 +150 h 1 M&h% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C't& h & M&x% h %\  A(O% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ()( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (~& h &  M&4& h &- M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &'( +166 h ( M& & h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +89 h ' A(j& h &  .t\SE.seek 13.71 Pucks below Top Plate A(u'n +125 h 'n A(%& h &  M&-& h & .t\Elbow.seek 2 M&( +7 h ( M&T& h & A(<& h &  .t\Forearm.seek 2 + 300 counts M&'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &#/ +150 h / ."downElevator"\SE.seek :down M&3& A(('X h & M&B% h %8 .t\Forearm.seek :retract M&\ '\ +91 h '\  .t\Elbow.seek CC M&o(ӷ +35 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&J& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&Z1 +150 h 1 M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'R  +151 h  .t\CSV.dial CTV C'`  +19 h  .t\CS.seek 5ml C'z'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'i' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'b'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%p  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%H +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%]  +150 h  .t\PS.seek :empty P%=' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'7  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'L @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'\\ ` +18 h ` .t\CSV.dial :air C'A @ +38 h @ .t\CS.seek 10ml C'a' +2647 h ' .t\CSV.dial CTV C'D  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C', @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&X% h %\  A( % h %X A(F. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &( M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&K& h & A(&& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +90 h ' A(k& h &( .t\SE.seek 5.71 Pucks below Top Plate A( ' +205 h ' M&v& h & A(/& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &N'C +25 h 'C .t\Elbow.hold M&!, +7 h , .t\Elbow.reconfigure TightElbow A(?- h -+ M&$&.,(\ #b .t\SE.seek 6.04 Pucks below Top Plate A("' +26 h ' M&w% h %C A(s& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&e'o +21 h 'o M&G& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &6' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(^' +36 h ' M&l& h & M&:% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +89 h '\  .t\Elbow.seek CC M&I(ӷ +143 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &( A(S& h &( M&y& h &ӷ M&C% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &*( +142 h ( M&|& h & A(;& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &1'C +93 h 'C .t\Elbow.hold M&g, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &21 +150 h 1 M&@% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&['o +20 h 'o M&0& h & A(+& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &n' +23 h ' A(d% h % ."downElevator"\SE.seek :down A(m'X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%}% h % .t\PS.seek 0.499ml P%M' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%h +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%t  +37 h .t\CBV.dial AIV C'x  +38 h .t\PRV.dial between CBV and air P%O  +19 h  .t\PRV.dial :air P%Y  +18 h  P%~% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %4' +251 h ' .t\PRV.dial :waste2 P%U @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %q' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&j& h & M&.% h %\  .t\Elbow.seek FlushPuck::Garage M&%(- +166 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&E/ +150 h / M&#& h &- M&3% h %η .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'u& h &W .tHand.open M&1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Z @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%9  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%R  +76 h  .tdelay 2 seconds +200 P%`% h % .t\PS.seek 1.499ml P%Q' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%)' +1094 h ' .t\PSV.dial :air P%P  +38 h  .t\PS.seek 0ml P%z' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%i +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%W  +38 h  .tdelay 2 seconds +200 P%V% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%p  +76 h .tdelay 2 seconds +200 P%H% h %r .t\PS.seek 0.475ml P%]' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%7  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%\\ +75 h .t\PTV.dial :PRV,avoiding: :puck P%A @ +75 h @ .t\PS.seek 0ml P%b' +2610 h ' .t\PSV.dial :air P%D  +75 h  .t\PS.seek 2.5ml P%a' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%, +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%X' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%F  +75 h P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%K% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %&'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'k { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %o' +1313 h ' .t\PSV.dial :air P%v  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :PML P%5 +38 h .t\PS.seek 0ml P%?' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'w& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C's& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*G @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%l +21 h  ."MFBPCR"delay 20 seconds +92 h P%:% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%_' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *N'6 +66 .t\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%e +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%"' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%6 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%!' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%r +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%S% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%y' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*C % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *|'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*I  +21 h  F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *@' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%0' +216 ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*d% h % ."MFBPCR"\AS.seek 0.469ml F*c' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%* +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%1% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%^  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*}9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*n n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*m% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%= +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%h' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial PSR P%x +113 h P%O% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%~  +38 h  .t\PS.seek 0ml P%M' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%U +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%4' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %j'k +2679 h 'k .t\PSV.dial :waste1 P%.  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %-' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*T I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *g'V +849 h ' .t\PSV.dial :air P%E  +113 h  .t\PS.seek 2.5ml P%#' +2610 h ' .t\PSV.dial :mixloop P%3 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %$' +5106 h ' .t\PSV.dial :air P%<  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %8' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*J  +43 h  F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *B'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*u% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%( +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +619 h ' ."MFBPCR"\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.seek :empty F*'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*9  +86 h  F*R% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *`' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*q  +43 h F*Q% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *P'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C')  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'V' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'p  'z  +38 h  +113  .t\CBV.dial :waste1 C'H @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*]% h %_ ."MFBPCR"\AS.seek 0.434ml F*7'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'\\'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*A  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*D'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*b n +108 h n F*,% h %& ."MFBPCR"\AS.seek 0.092ml F*a'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C' '4 +2647 h '4 C'F& h &W #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'&( +416 h ( M&v& h &ӷ M&o% h % .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&l1 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C' & h & M& % h %\  A(>% h %X A({. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (_( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (e& h &  M&& h &- M&6% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &"( +166 h ( M&& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &r' +90 h ' A(S& h &  ."WCR"\SE.seek 12.71 Pucks below Top Plate A(N' +136 h ' A(!& h &  M&C& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&;& h & A(I& h &  ."WCR"\Forearm.seek 2 + 300 counts M&2'8 +23 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down A('X M&|& h & M&+% h %8 ."WCR"\Forearm.seek :retract M&d'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&@& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +35 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +13 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&^1 +130 h 'R ."MFBPCR"\ATV.dial :air2 F*[  +20 h 1 M&}% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *m' +89 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)c+ +6 h + S)=% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*e% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %  ."MFBPCR"\AS.seek 0.604ml F*/'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +21 h F*C% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*;' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*I n +86 h n ."MFBPCR"\ASV.dial :wasteR F*?  +43 h  ."MFBPCR"\AS.seek 0ml F*y'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*" % +108 h % F*r% h %V ."MFBPCR"\AS.seek 0.59ml F*@' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +108 h n ."MFBPCR"\ASV.dial :wasteR F*N% h %  ."MFBPCR"\AS.seek 0.09ml F*2' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*d' +625 h ' ."MFBPCR"\ATV.dial :air3 F*a % +21 h % F*&% h % ."MFBPCR"\AS.seek 0.13ml F*^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*[% h % ."MFBPCR"\AS.seek 0.23ml F*=' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*0'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*_% h %X ."MFBPCR"\AS.seek 0.24ml F*c' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*O n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*x  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *Y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*h @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*.  +43 h  #"MFBPCR"\Swish #1 of 2 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *T'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*E'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*4% h %(A ."MFBPCR"\AS.seek 0.6ml F*#'\  +1031 h '\  F*<% h %\  ."MFBPCR"\AS.seek 0.5ml F*$'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*J% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *-' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*3 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0317h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*u  +64 h  ."MFBPCR"\ARV.dial :carrier F*(  +11 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*%'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +21 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*`  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*)' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*W  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*H % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +107 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*P'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*b' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*7  +43 h  ."MFBPCR"\ASV.dial :waste1 F*D  +65 h ."MFBPCR"\ARV.dial :air1 F*,  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226097 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'F @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'' @ +75 h @ +50 S)K% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C'v% h % ."WCR"\CSV.dial between air and CTV C'\ ` +18 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'w  'o  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'X'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'l  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 's' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'5  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C':  +37 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'{' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +815 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)>% h % ."WCR"\CBV.dial between CSV and waste2b C'6  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'S  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '!'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'C  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '/' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'?  +38 h  C';% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'y' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'" @ +75 h @ +50 S)r% h % ."WCR"\CBV.dial between CSV and waste2b C'+  +94 h  C'*% h % ."WCR"\CSV.dial between air and CTV C'N ` +57 h ` ."WCR"\CS.seek 7.288ml C'@' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'2  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'a' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'&  +150 h C'd& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&}% h %\  A([% h %X A(^. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&=& h &ӷ M&1% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &_( +143 h ( M&0& h & A(O& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(c& h &( ."WCR"\SE.seek 6.71 Pucks below Top Plate A(~' +196 h ' M&t& h & A(Y& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C ."WCR"\Elbow.hold M&M, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(.' +27 h ' M&j% h %C A(U& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M& 'o +21 h 'o M&T& h & A(4& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &E' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A(<'w +36 h 'w M&#& h & M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +90 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +142 h (ӷ M&-& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&u' +91 h ' ."WCR"Hand.close M&(/ +150 h / ."WCR"\CC.seek :open C'B( +416 h ( A(g& h &( A(%& h &( M&m& h &ӷ M&Z% h % ."WCR"\Forearm.seek :retract M&9'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&q& h & A(Q& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &R'C +92 h 'C ."WCR"\Elbow.hold M&`, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&% h %C A(W& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&p'o +21 h 'o M&)& h & A(H& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &V' +22 h ' A(% h %w ."downElevator"\SE.seek :down A(]'X +100 (L% h % M&z% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +82 h 'X ."downElevator"\SE.coast A(5 +7 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&A% h %\  .t\Elbow.seek FlushPuck::Garage M&P(- +166 h (- M&7& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.seek :garage M&,'η +91 h 'η .tHand.close M&/ +150 h / M&b& h &- M&\\% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +90 h ' .t\CC.seek :closed C'K(W +416 h (W C'& h &W .tHand.open M&v1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'w& h &W C'o% h % .t\CSV.dial CSR C'  +38 h  C'l% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'X'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '5% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ':'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 's'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C' % h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '6'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'S  +37 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'C'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'!& h &W C'I% h %4 .t\CSV.dial :flush C'?  +76 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'y% h %p .t\CS.seek 7ml C'"'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'r  +37 h  .t\CBV.dial :waste2b C'+  +75 h  .t\CS.seek 0ml C'*'4 +766 h '4 #t\Flushing upper manifold C'N& h &W C'|% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'2% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& 'd  +37 h +76 .t\CTV.dial :air C'}  +150 h .t\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'='4 +507 h '4 C'1& h &W C'0% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C'c'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C't% h %0 .t\CS.seek 5ml C'Y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'n'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C'U'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'T& h &W C'4% h %4 .t\CSV.dial CSR C'#  +151 h  C'J% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'x'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'_' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'~ +112 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'-' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'.  +113 h  C'E% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'M'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '  +37 h  +113  .t\CBV.dial :waste1 C'g @ +38 h @ .t\CS.seek 0ml C'%'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'm  +38 h  C'Z% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'q'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +709 h ' ."connect"\CSV.dial CTV C'u  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'3  +75 h  .t\CS.seek 0ml C'i'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ')'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '9  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'L'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'& h &W C'A% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'7% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'V +113 h .t\CTV.dial :air .\CS.seek 0ml C']'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'` +38 h .t\CS.seek 0ml C''4 +506 h '4 C'b& h &W C'\\% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C','0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C' % h %0 .t\CS.seek 5ml C'P'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'v'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'\ & h &W C'w% h %4 .t\CSV.dial :flush C'o  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C''% h %p .t\CS.seek 7ml C'a'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'5  'X  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C':'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C' % h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C's'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'S  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'e& h &W C'!% h %4 .t\CSV.dial CSR C'I  +37 h  C'?% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'C'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'/' +416 h ' ."connect"\CSV.dial CBV C'y .t\CBV.dial CSV C';  +37 h +76 .t\CTV.dial :intake C'r  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'+' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'"  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'N'4 +606 h '4 .t\CSV.dial CSR C'|  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'*'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +1373 h ' .t\CSV.dial CBV C'2 +112 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&d& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &&(ӷ +6 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&[' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'^( +416 h ( M&1& h &ӷ M&0% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &=(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &c'η +91 h 'η .tHand.open M&Y1 +150 h 1 M&n% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&T1 +2 h 1 M&4& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &#(- +7 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&x'η +91 h 'η .tHand.close M&~/ +150 h / M&& h &- M&_% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &-(ӷ +264 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'M(W +416 h (W C'& h &W .tHand.open M&$1 +150 h 1 M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'm& h &W C'Z% h %4 .t\CSV.dial CSR C'%  +113 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'u'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '3% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Q'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +2010 h '4 #t\Purging external Intake C'i& h &W C')% h %4 .t\CSV.dial :flush C'9  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'H  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'L'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'z& h &W C'% h %4 .t\CSV.dial :flush C'A  +76 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'<% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'p +113 h .t\CTV.dial :air .\CS.seek 0ml C'V'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'] +38 h .t\CS.seek 0ml C'`'4 +506 h '4 C'& h &W C'b% h %4 .t\CSV.dial :flush C'\\  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'F% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C','4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&B& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\ (ӷ +6 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C''( +416 h ( M&& h &ӷ M&5% h % .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +90 h 'η .tHand.open M&>1 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST04-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:48:53CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST04-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0317h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*S % +86 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +21 h F*I% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*;'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*|% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*1% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*Y% h %m ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0319h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ARV.dial :carrier F*.  +11 h  F*E% h %V ."MFBPCR"\AS.seek 0.5ml F*#'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*^ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*g  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *%'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*q  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*3  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Q  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +108 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0319h1500ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*D  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *\\'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*,'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +65 h  F*l% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *-'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +31 h  ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*G'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +21 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0321h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*N% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *K'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*1 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*[' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.53ml F*&' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*Y % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*n  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*4 n +21 h n ."MFBPCR"delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*c  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*_  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F*.'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0321h1500ml.pcr F*#% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*$% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*i  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V @CEST1538604016.16 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0400h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*l  +42 h  ."MFBPCR"\ARV.dial :carrier F*B  +11 h  F*-% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *a'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*X I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*:' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*G' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*S  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*!  +108 h  ."MFBPCR"delay 7 seconds +700 F*s% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*r' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*;  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0400h1500ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*0% h %n ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*.'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0402h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*Q  +11 h  F*W% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*A I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*p% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*h % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*D  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +22 h  ."MFBPCR"delay 7 seconds +700 F*b% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*P  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*,  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*- % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0402h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*o @@T *(  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*M  +151 h F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *{' +423 h ' ."MFBPCR"\ATV.dial :ASV F*\ n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*>  +43 h  F*v% h % ."MFBPCR"\AS.seek 0.62ml F*X'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *6' +423 h ' ."MFBPCR"\ATV.dial :air3 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*e' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h % H ."MFBPCR"\AS.seek 0.735ml F*?' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*C% h % Ű ."MFBPCR"\AS.seek 0.72ml F*;' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*" n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*+% h % zG ."MFBPCR"\AS.seek 0.365ml F*r' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*| % +43 h % F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*d% h % ."MFBPCR"\AS.seek 0.579ml F**' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +21 h F*k% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*0  +22 h  ."MFBPCR"\AS.seek 0ml F*K'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*& % +108 h % F*[% h %V ."MFBPCR"\AS.seek 0.01ml F*n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h % ."MFBPCR"\AS.seek 0.58ml F*4' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h % q ."MFBPCR"\AS.seek 0.605ml F*~'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +21 h F*_% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*c' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*='V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *#'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*E  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *.'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*^ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +202 h 'R ."MFBPCR"\SPE.coast F*Z  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*$'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*m n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *q' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*u I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*i  +43 h  F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*W  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *)'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*H% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*z  +43 h  F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *p': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*h% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *V'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*< n +65 h n F*D% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*b  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *\\'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*` n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*F  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *,' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F* % h % ."MFBPCR"\AS.seek 0.96ml F*B'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*- % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %0 ."MFBPCR"\AS.seek 0.985ml F*l' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*5% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*\ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *v'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *a'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*:% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*G I +43 h I ."MFBPCR"\AS.seek 0.02ml F*6' +9880 h ' ."MFBPCR"\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*!% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*I  +21 h ."MFBPCR"\ATV.dial :air3 F*e % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *C'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*"% h %Z ."MFBPCR"\AS.seek 0.81ml F*+'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +64 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*K% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*n'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*T% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast F*#,,'T(20( b ."MFBPCR"\AS.seek 0ml F*c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *.'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*g % +108 h % ."MFBPCR"\AS.seek 0ml F*m'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + &6 C'5 P%5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990711 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )  h  C' '% h  P% %$ h  F* *u h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *q  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)i% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'9& S)#.0X 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:49:53CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'5& h & .tHand.open M&l1 +150 h 1 M&\ & h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &>(- +166 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&X/ +150 h / M&& h &- M&:% h %η .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &S(ӷ +264 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &s' +91 h ' .t\CC.seek :closed C'!(W +416 h (W C'6& h &W .tHand.open M&1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'y& h &W C'% h %4 .t\CSV.dial CSR C'?  +150 h  C'"% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'C'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ';% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'+ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '|'4 +2009 h '4 #t\Purging external Intake C'/& h &W C'r% h %4 .t\CSV.dial :flush C'2  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'd' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N '@  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'}'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'*  +75 h #t\Flushing upper manifold C'1& h &W C'k% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'0'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'K% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'& .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'['4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'O'4 +507 h '4 C't& h &W C'J% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.seek 3ml C'n'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'_'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'c'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +7 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&^& h &ӷ M&g% h % .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&f1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:49:58CEST30-Sep-18\\n{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:49:53CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*8% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'o& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M& % h %\  A(%% h %X A($. h ., A(u& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (q& h &  M&& h &- M&i% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &9( +6 F*3% h %&K ."readyMFB"\ATV.dial :waste2 F*W % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +160 h ( M&Q& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +89 h ' A()& h &  .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +146 h 'Ɛ A(& h &  M&L& h & .t\Elbow.seek 2 M&z( +7 h ( M&A& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&h'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &% h %W .tIntake.select :close S)0 +6 )a% h %W .t\SSV.dial :exhaust S)B  +75 h  .t\Exhaust.select :open S)@ +7 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9 ,d +100 C'a9 h 9$0d +100 C'B9 h 9<4d +100 C' 9 h 9S8d +100 C'I9 h 9kd +100 C'k9 h 9Td +100 C'9 h 9kd +100 C'9 h 9d +100 C'}9 h 9d +100 C'K9 h 9d +100 C'09 h 9d +100 C'&9 h 9d +100 C'9 h 9d +100 C'[9 h 9 d +100 C'|9 h 9 d +100 C'19 h 96d +100 C'Y9 h 9Ld +100 C't9 h 9bd +100 C'J9 h 9xd +100 C'9 h 9d +100 C'O9 h 9d +100 C'U9 h 9d +100 C'49 h 9d +100 C'T9 h 9d +100 C'n9 h 9d +100 C'~9 h 9d +100 C'9 h 9(d +100 C'=9 h 9>d +100 C'_9 h 9Td +100 C'#9 h 9id +100 C'9 h 9d +100 C'E9 h 9d +100 C'x9 h 9d +100 C'9 h 9d +100 C'e9 h 9d +100 C'!9 h 9d +100 C'g9 h 9  d +100 C''9 h 9 d +100 C'S9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'f9 h 9 j d +100 C'^9 h 9 $d +100 C'j9 h 9 (d +100 C'.9 h 9 ,d +100 C'89 h 9 0d +100 C' 9 h 9 4d +100 C'%9 h 9 8d +100 C'$9 h 9 % h %p .t\CS.seek 7ml C'a'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'B @ +38 h @ .t\CS.seek 10ml C' ' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'I ` +132 h ` #tLysing Complete C'y& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&% h %\  A(?% h %X A(6. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &( M&X& h &ӷ M&;% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +143 h ( M&+& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +89 h ' A(r& h &( .t\SE.seek 7.71 Pucks below Top Plate A(2' +185 h ' M&"& h & A(@& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &N'C +25 h 'C .t\Elbow.hold M&*, +7 h , .t\Elbow.reconfigure TightElbow A(d- M&$&.,(\ #b h -+ .t\SE.seek 8.04 Pucks below Top Plate A(c'w +27 h 'w M&k% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&}& h & A(K& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &0' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A(&'f +36 h 'f M&& h & M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +90 h '\  .t\Elbow.seek CC M&1(ӷ +142 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&J' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'O( +416 h ( A(U& h &( A(4& h &( M&T& h &ӷ M&n% h % .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&=& h & A(_& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &#'C +93 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &E1 +150 h 1 M&x% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&e'o +21 h 'o M&!& h & A(g& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &'' +22 h ' A(S% h %f ."downElevator"\SE.seek :down A('X +100 (% h % M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +72 h 'X ."downElevator"\SE.coast A(5 +17 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'j @ +19 h @ .t\CS.seek 10ml C'.' +7 h ' .t\CSV.dial between air and CTV C'8 ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'%& h & .tHand.open M&$1 +2 h 1 M&u& h & M&q% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &- .t\Forearm.seek :garage M&W'η +91 h 'η .tHand.close M&o/ +150 h / M&& h &- M&m% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &A' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&<1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%9' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%H  +37 h  .t\PS.seek 0ml P%V' +1597 h ' .t\PSV.dial PSR P%w +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%b' +2610 h ' ."connect"\PRV.dial CBV P%h  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P%z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 2.5ml P%Z' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%5' +2610 h ' .t\PSV.dial :air P%p  +37 h  .t\PS.seek 2.5ml P%F' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +7 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'7( +416 h ( M&& h &ӷ M& % h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&B1 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'I& h & M&y% h %\  A(% h %X A(?. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (6( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (s& h &  M&X& h &- M&;% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &+( +166 h ( M&& h & A(r& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &`' +90 h ' A("& h &  .t\SE.seek 10.71 Pucks below Top Plate A(@' +155 h ' A(R& h &  M&d& h & .t\Elbow.seek 2 M&k( +6 h ( M&& h & A(}& h &  .t\Forearm.seek 2 + 300 counts M&K'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(['X M&/& h & M&C% h %8 .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.seek CC M&N(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'c(W +416 h (W .tHand.open M&01 +150 h 1 M&*% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'|  +150 h  .t\CSV.dial CTV C'(  +19 h  .t\CS.seek 5ml C'U'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'4' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'T'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%n  +37 h  .t\PS.seek 2.5ml P%=' +2610 h ' .t\PSV.dial :mixloop P%_ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%J  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%1 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%E  +150 h  .t\PS.seek :empty P%x' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'!  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'g @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'S  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'~ ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'f  +38 h  .t\CS.seek 0ml C'j'4 +2647 h '4 .t\CBV.dial :waste1 C'8 @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'%& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&$% h %\  A(u% h %X A(q. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (#(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (e& h &( M&&& h &ӷ M&'% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &i( +142 h ( M&3& h & A(& h &( .t\Forearm.reconfigure TightForearm M&#Q@2^d^\ b .t\Forearm.seek 1 + 700 counts M&' +90 h ' A( & h &( .t\SE.seek 8.71 Pucks below Top Plate A(' +175 h ' M&o& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &m'C +25 h 'C .t\Elbow.hold M&^, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(W- h -+ .t\SE.seek 9.04 Pucks below Top Plate A( 'f +27 h 'f M&L% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&)'o +21 h 'o M&O& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +22 h ' .t\SE.seek 10.04 Pucks below Top Plate A('U +37 h 'U M&& h & M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +89 h '\  .t\Elbow.seek CC M&H(ӷ +142 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'( +416 h ( A(z& h &( A(& h &( M&b& h &ӷ M&-% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +143 h ( M&Z& h & A(p& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &5'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &F1 +150 h 1 M&% h %C A(v& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&{'o +21 h 'o M&& h & A(Q& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\\' +22 h ' A(% h %U ."downElevator"\SE.seek :down A('X +100 ( % h % M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +61 h 'X ."downElevator"\SE.coast A(5 +29 h '\  .t\SC.coast .\PSV.dial :air A(6 P%>  +112 h  P%,% h % .t\PS.seek 0.499ml P%B' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%\ +38 h .t\PRV.dial :air P%P  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%I  +38 h .t\CBV.dial AIV C'y  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%?  +18 h  P%s% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %X' +251 h ' .t\PRV.dial :waste2 P%; @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %G' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&r1 +2 h 1 M&"& h & M&:% h %\  .t\Elbow.seek FlushPuck::Garage M&R(- +166 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&}'η +91 h 'η .tHand.close M&a/ +150 h / M&& h &- M&/% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&`& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'K(W +416 h (W C'@& h &W .tHand.open M&k1 +150 h 1 M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  C'7& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%0 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%*  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%|  +76 h  .tdelay 2 seconds +200 P%(% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%6' +1094 h ' .t\PSV.dial :air P%.  +38 h  .t\PS.seek 0ml P%U' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%4 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%n  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %_'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%J  +76 h .tdelay 2 seconds +200 P%1% h %r .t\PS.seek 0.475ml P%E' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%=  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%!  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%g  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %S' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%~ +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%T' +2610 h ' .t\PSV.dial :air P%f  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%8 +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%%  +75 h  .t\PS.seek 2.5ml P%$' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%u +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%q  +75 h P%e% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %&' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'' & +50 h & .t\AWV.select :waste C' +7 P%3% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %N' +1313 h ' .t\PSV.dial :air P%o  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%W +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'L& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*O @@T *  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +91 h P%D% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*i I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *m'6 +66 .t\PSV.dial :waste1 P%9  +38 h  .t\PS.seek 0ml P%w' +1597 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%< +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%^' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*h  +22 h ' .t\PSV.dial PSR P%A +21 h  F*c% h %6 ."MFBPCR"\AS.seek 0.329ml F*z'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%b' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*- % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%p  +37 h  .t\PS.seek 0ml P%V' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*H  +21 h  F*F% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%v +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*Q I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%l +113 h P%5% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%>' +739 h ' ."MFBPCR"delay 5 seconds +500 F*,9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*\\ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*#'% +336 h ' .t\PSV.dial PSR P%x +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%\ ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%I' +2609 h ' .t\PSV.dial PSR P%y +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%?' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%s  +38 h  .t\PS.seek 0ml P%B' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%; +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%X' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %"'k +2679 h 'k .t\PSV.dial :waste1 P%:  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %d' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%a  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%/ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%}  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %`' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*Y  +43 h  F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *C'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*@% h %Q ."MFBPCR"\AS.seek 0.01ml F*k' +171 h ' .t\PSV.dial :mixloop P%[ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %7' +619 h ' ."MFBPCR"\ATV.dial :waste F*0 n +128 h n ."MFBPCR"\AS.seek :empty F*t'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F**  +86 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *(' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*G  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *.'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'6  +37 h  C'4% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'n'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'J  'U  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +38 h @ .t\CS.seek 0ml C'_'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*=  +22 h ."MFBPCR"delay 3 seconds +300 F*E% h %_ ."MFBPCR"\AS.seek 0.434ml F*!'ݏ +110 h '4 .t\CSV.dial CSR C'g  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'~'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*S% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*f'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*T n +107 h n F*8% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'%' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'u'4 +2647 h '4 C'q& h &W #tGaraging FlushPuck in Collection Clamp M&e& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +7 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( M&o& h &ӷ M&N% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&D% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'i& h & M&% h %\  A(9% h %X A(]. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ()& h &  M&w& h &- M&<% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +166 h ( M&h& h & A(c& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &A' +90 h ' A(& h &  ."WCR"\SE.seek 9.71 Pucks below Top Plate A(m' +165 h ' A(^& h &  M&-& h & ."WCR"\Elbow.seek 2 M&z( +7 h ( M&p& h & A(H& h &  ."WCR"\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &b/ +89 h '&0 ."MFBPCR"delay 3 seconds +61 h / ."downElevator"\SE.seek :down M&v& A(Z'X h & M&Q% h %8 ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&V(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +5 ."MFBPCR"\ATV.dial :waste2 F*l % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +43 h ' ."WCR"\CC.seek :closed C' (W +416 h (W ."WCR"Hand.open M&1 +100 h 'R ."MFBPCR"\ATV.dial :air2 F*{  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *,' +28 h 1 M&\\% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)x% h % C'>& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'P  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h % ."MFBPCR"\AS.seek 0.255ml F*5'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *?'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*\ @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*# I +86 h I ."MFBPCR"\AS.seek 0ml F*r'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*;'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*:  +43 h  #"MFBPCR"\Swish #1 of 2 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*a'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*X% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*}% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*Y% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *d' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*/ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0417h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*@  +64 h  ."MFBPCR"\ARV.dial :carrier F*[  +11 h  F*C% h %V ."MFBPCR"\AS.seek 0.5ml F*R'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*, n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*G  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*(  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*4% h %Fj ."MFBPCR"\AS.seek 0.524ml F*6' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*n  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*j' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*1 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*U'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*E  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*= n +21 h n ."MFBPCR"delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.6ml F*.'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*S  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*T' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 F*f  +65 h ."MFBPCR"\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*%'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*e % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226127 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C''  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'q @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'+ @ +75 h @ +50 S)3% h % ."WCR"\CBV.dial between CSV and waste2b C'K  +94 h  C'o% h % ."WCR"\CSV.dial between air and CTV C'2 ` +19 h ` ."WCR"\CS.seek 7.288ml C'&' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'L  'N  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '$'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'D  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C']' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'i @ +75 h @ +50 S)9% h % ."WCR"\CBV.dial between CSV and waste2b C'<  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'w ` +57 h ` ."WCR"\CS.seek 7.288ml C'c' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'h  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '^'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'-  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'H  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'p% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'b' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)A% h % ."WCR"\CBV.dial between CSV and waste2b C'Q  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C'l ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'F  ."WCR"\CSV.dial CTV C'z  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Z'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'm  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(\\% h %X A({. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (>(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (P& h &( M&x& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&5& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +89 h ' A(& h &( ."WCR"\SE.seek 9.71 Pucks below Top Plate A(s' +166 h ' M&I& h & A(?& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &#'C +25 h 'C ."WCR"\Elbow.hold M&B, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(r- h -+ ."WCR"\SE.seek 10.04 Pucks below Top Plate A(:'U +27 h 'U M&"% h %C A(;& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&'o +21 h 'o M&& h & A(X& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &a' +22 h ' ."WCR"\SE.seek 11.04 Pucks below Top Plate A(}'D +36 h 'D M&& h & M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&/(ӷ +142 h (ӷ M&d& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&@' +91 h ' ."WCR"Hand.close M&[/ +150 h / ."WCR"\CC.seek :open C'C( +416 h ( A(& h &( A(R& h &( M&,& h &ӷ M&0% h % ."WCR"\Forearm.seek :retract M&*'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &k( +142 h ( M&G& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &|'C +93 h 'C ."WCR"\Elbow.hold M&(, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &41 +150 h 1 M&t% h %C A(n& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&J'o +21 h 'o M&6& h & A(1& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(j% h %D ."downElevator"\SE.seek :down A(E'X +100 (g% h % M&U% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +52 h 'X ."downElevator"\SE.coast A(5 +37 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'_& h & .tHand.open M&1 +2 h 1 M&S& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&.(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.seek :garage M&8'η +91 h 'η .tHand.close M& / +150 h / M&T& h &- M&~% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'K& h &W .tHand.open M&o1 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'N& h &W C'L% h % .t\CSV.dial CSR C'&  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'$'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'W% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'D'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C']& h &W C'i% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '<'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'w% h %p .t\CS.seek 7ml C')'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'h  +38 h  +113  .t\CBV.dial :waste1 C'c @ +75 h @ .t\CS.seek 0ml C'-'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'^& h &W C'H% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C' 'i +415 h 'i ."connect"\CSV.dial CTV C'A  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'Q  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing upper manifold C'l& h &W C'v% h %4 .t\CSV.dial :flush C'z  +76 h  .t\CS.seek 5ml C'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'm% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +37 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'{'0 +507 h '0 .t\CSV.dial :waste C'P +37 h .t\CS.seek 0ml C'x'4 +507 h '4 C'& h &W C'5% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'I% h %0 .t\CS.seek 5ml C'?'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'r'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'" +76 h .t\CS.seek 0ml C';'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'X% h %4 .t\CSV.dial CSR C'  +151 h  C'Y% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'y'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C's +113 h .t\CTV.dial :intake C'#  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'd' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C':  +113 h  C'a% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'B'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'R'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C',  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'G'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV C'@  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'/  +75 h  .t\CS.seek 0ml C'4'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C't  +38 h  C'n% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '6'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +710 h ' ."connect"\CSV.dial CBV C'j .t\CBV.dial CSV C'*  +38 h +75 .t\CTV.dial :air C'k  +151 h .t\CS.seek 0ml C'g'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +507 h '0 .t\CSV.dial :waste C'_ +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'S& h &W C'% h %4 .t\CSV.dial :flush C'|  +38 h  .t\CS.seek 5ml C'J'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'!% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C'E'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'>'0 +507 h '0 .t\CSV.dial :waste C'( +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'T& h &W C'~% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.seek 3ml C'8'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'e'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'K +75 h .t\CS.seek 0ml C'o'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'2& h &W C'N% h %4 .t\CSV.dial :flush C'L  +37 h  .t\CS.seek 5ml C'&'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'+% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  '$  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing lower manifold's waste2b port C']& h &W C'i% h %4 .t\CSV.dial :flush C'9  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'w% h %p .t\CS.seek 7ml C'<'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'h  +76 h  .t\CS.seek 0ml C'c'4 +765 h '4 #tDisinfecting external Intake with kill C')& h &W C'^% h %4 .t\CSV.dial CSR C'H  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'-'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'b .t\CBV.dial CSV C'p  +37 h +76 .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'Q' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'l'4 +606 h '4 .t\CSV.dial CSR C'v  +113 h  C'z% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'm +113 h .t\CS.seek 0ml C'F'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&\\' +91 h ' .tHand.close M&P/ +150 h / .t\CC.seek :open C'{( +416 h ( M&& h &ӷ M&5% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +264 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&?1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&X& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.seek :garage M&y'η +91 h 'η .tHand.close M&s/ +150 h / M&#& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'B(W +416 h (W C'& h &W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Flushing the Collection Valve::Manifold C',& h &W C'0% h %4 .t\CSV.dial CSR C'R  +113 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'@'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '/% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +462 h '[ .t\CSV.dial :waste C't +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +2010 h '4 #t\Purging external Intake C'4& h &W C'6% h %4 .t\CSV.dial :flush C'*  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k +75 h .t\CTV.dial :intake C'1  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'g'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'_  +75 h #t\Flushing upper manifold C'U& h &W C'S% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'J +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C'E +38 h .t\CS.seek 0ml C'('4 +506 h '4 C'>& h &W C'T% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.seek 3ml C'='0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'q% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'8'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'.'0 +507 h '0 .t\CSV.dial :waste C'e +75 h .t\CS.seek 0ml C'K'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&C& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +6 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.seek CC M&o' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'+( +416 h ( M&&& h &ӷ M&W% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &i'η +91 h 'η .tHand.open M&91 +150 h 1 M&D% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST05-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:51:41CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST05-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0417h1500ml.pcr F*w% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *)'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*p'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*A% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +64 h  F*v% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *z'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F*P % +86 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*5'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0419h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ARV.dial :carrier F*:  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +21 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *R'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*/  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*@% h % ."MFBPCR"\AS.seek 0.53ml F*t' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.536ml F*6'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F**  +108 h  ."MFBPCR"delay 7 seconds +700 F*n% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*1 n +22 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*U  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*}% h % \\ ."MFBPCR"\AS.seek 0.326ml F*|'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0419h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +21 h % ."MFBPCR"delay 3 seconds +300 F*u% h %> ."MFBPCR"\AS.seek 0.835ml F*8'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +21 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F*C'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*N  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*L  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*[' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*]  +31 h  ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*i% h % ."MFBPCR"\AS.seek 0.81ml F*O'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +21 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0421h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*z  +10 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *3'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*V I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*? % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*r  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*X n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0421h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *,'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +21 h F*F% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +65 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*_  +31 h  ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *}' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*J% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*|'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*T'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V @CEST1538690416.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0500h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*C  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*['(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*]  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*2  +22 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*$ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*D' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*w n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*O' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*h  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +21 h n ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*-'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*b% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*p  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0500h1500ml.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*m % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*Z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*5% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*; % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*#% h % ."MFBPCR"\AS.seek 0.81ml F*a'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0502h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*n% h %V ."MFBPCR"\AS.seek 0.5ml F*6'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*_  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*J% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*\ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*!' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*|'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*E  +22 h  ."MFBPCR"delay 7 seconds +700 F*T% h %< ."MFBPCR"\AS.seek 0.594ml F*('\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.6ml F*='\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*u  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.65ml F*e' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*.  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*N% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0502h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*L @@T *[  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*B  +151 h F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *]' +423 h ' ."MFBPCR"\ATV.dial :ASV F*2 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*9  +43 h  F*o% h % ."MFBPCR"\AS.seek 0.62ml F*$'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*i n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *w'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*%'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*D % +43 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *<' +423 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*^' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*)' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*-% h % Ű ."MFBPCR"\AS.seek 0.72ml F*p' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Q% h % zG ."MFBPCR"\AS.seek 0.365ml F*A' +7161 h ' ."MFBPCR"\ATV.dial :waste F*z n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*v % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*P'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*Z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*V n +86 h n ."MFBPCR"\ASV.dial :wasteR F*5  +22 h  ."MFBPCR"\AS.seek 0ml F*3'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*\\% h %V ."MFBPCR"\AS.seek 0.01ml F*r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*X' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*s'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*y n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*#  +21 h  ."MFBPCR"\AS.seek 0ml F*x'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*a  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *:'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*{ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*0  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*, n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *G' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*@ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*4  +43 h  F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*n  +64 h  F*t% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *6'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*j n +65 h n F*1% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *k' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*_ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*U  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*S  +65 h  F*\ % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*} n +65 h n F*f% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *>' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*! I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*T  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *|': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *~'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*( n +151 h n ."MFBPCR"\AS.seek 0ml F*u'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*q  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*= % +43 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*e% h % ."MFBPCR"\AS.seek 0.96ml F*C'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*W% h % ."MFBPCR"\AS.seek 0.97ml F*&'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*2 I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *o'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *i'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*w  +43 h  F*D% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *%' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F*<' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*^% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*H  +22 h ."MFBPCR"\ATV.dial :air3 F*) % +43 h % F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*Q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*z% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*m  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*\\% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*X'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *:'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*,'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast .\CS.coast S)5 C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' 'R h  P% %  h  F* *@ h  C' 9\ ' 0\ P% 9\ % 2\ % *\ C' -\ P% 0\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *G  h  .t\SPE.configure SPEconfig F*\ 2 *F% h %V .t\AS.configure ASconfig S)4% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'*& S)#.0X/ .t\Elbow.jog 1500 M&T* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b S)#.0X 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:52:41CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #5) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&1 +150 h 1 M&2& h &M M&&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &9(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &i'η +91 h 'η .tHand.close M&$/ +150 h / M&w& h &- M&D% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'<& h &W .tHand.open M&1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'b& h &W C'c% h %4 .t\CSV.dial CSR C'  +150 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '-'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'p% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'z'[ +463 h '[ .t\CSV.dial :waste C'Q +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'v'4 +2009 h '4 #t\Purging external Intake C'& h &W C'A% h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'l  h +75 .t\CTV.dial :intake C''  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C' % h %4 .t\CSV.dial :flush C'P  +75 h  .t\CS.seek 5ml C'5'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'3% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'\\'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'I& h &W C'Y% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'r'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'X% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C's'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +507 h '0 .t\CSV.dial :waste C'# +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +7 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'0( +416 h ( M&{& h &ӷ M&% h % .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&K1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:52:46CEST30-Sep-18\\n{1 => 12, 2 => 10, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:52:41CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'L& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(R% h %X A( . h ., A(@& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (G& h &  M&F& h &- M&4% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &*( +6 F*/% h %&K ."readyMFB"\ATV.dial :waste2 F*n % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *t' +160 h ( M&& h & A(j& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(6& h &  .t\SE.seek 8.71 Pucks below Top Plate A(_' +176 h ' A(k& h &  M&g& h & .t\Elbow.seek 2 M&U( +6 h ( M&& h & A(\ & h &  .t\Forearm.seek 2 + 300 counts M&'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &}/ +150 h / ."downElevator"\SE.seek :down A(S'X M&f& h & M&% h %8 .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.seek CC M&((ӷ +85 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'd(W +416 h (W .tHand.open M&[1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +6 h + S)u% h % C'W& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % .t\CS.seek 6ml C'0' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'[ +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b 'J' +710 h ' .t\CSV.dial CSR C'u  +150 h  C'W% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '8' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %  .t\CS.seek 4.5ml C'q'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'=  +38 h  .t\CS.seek 1.275ml C'&' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'2   '!9 h 9d +100 C'o9 h 9d +100 C']9 h 91d +100 C'9 h 9J d +100 C'w9 h 9bd +100 C't9 h 9zd +100 C'D9 h 9d +100 C'e9 h 9d +100 C'.9 h 9 d +100 C'O9 h 9$d +100 C'%9 h 9(d +100 C'99 h 9 ,d +100 C'9 h 9$0d +100 C'B9 h 9<4d +100 C'i9 h 9S8d +100 C'H9 h 9kd +100 C' 9 h 9Td +100 C''9 h 9kd +100 C'P9 h 9d +100 C'9 h 9d +100 C'39 h 9d +100 C'59 h 9d +100 C'9 h 9d +100 C'V9 h 9d +100 C'\\9 h 9 d +100 C'v9 h 9 d +100 C'9 h 96d +100 C'?9 h 9Ld +100 C'I9 h 9bd +100 C'Y9 h 9xd +100 C'Z9 h 9d +100 C'9 h 9d +100 C';9 h 9d +100 C'X9 h 9d +100 C'9 h 9d +100 C'r9 h 9d +100 C's9 h 9d +100 C'#9 h 9(d +100 C'x9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'a9 h 9d +100 C'y9 h 9d +100 C'9 h 9d +100 C')9 h 9d +100 C'^9 h 9d +100 C'9 h 9  d +100 C'9 h 9 d +100 C'h9 h 9 *d +100 C'9 h 9 ?d +100 C'+9 h 9 Td +100 C'K9 h 9 j d +100 C'{9 h 9 $d +100 C'"9 h 9 (d +100 C':9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'R9 h 9 8d +100 C' 9 h 9 9 h 9 Xd +100 C'S9 h 9 kd +100 C'[9 h 9 ~d +100 C'09 h 9 d +100 C'u9 h 9 d +100 C'W9 h 9 d .t\CH.hold 5:00,85C C'J   P h  +30000  C' 9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'8'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'= @ +113 h @ .t\CSV.dial :air C'q @ +37 h @ .t\CS.seek 5ml C'!'p +1373 h 'p .t\CSV.dial CTV C'o  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'] @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'w @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C't'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'D +113 h .t\CBV.dial CSV C'e  +19 h C'.% h %4 .t\CS.seek 5ml C'O'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'%  +38 h C'9% h %p .t\CS.seek 7ml C''i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'B @ +37 h @ .t\CS.seek 10ml C'i' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'H ` +132 h ` #tLysing Complete C'b& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&c% h %\  A(% h %X A(<. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&$& h &ӷ M&p% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &-( +143 h ( M&Q& h & A(z& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(A& h &( .t\SE.seek 10.71 Pucks below Top Plate A(m' +155 h ' M& & h & A(l& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ .t\SE.seek 11.04 Pucks below Top Plate A('D +27 h 'D M& % h %C A('& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&P'o +21 h 'o M&& h & A(3& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &5' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A('3 +36 h '3 M&V& h & M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&Y' +91 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C'( +416 h ( A(;& h &( A(X& h &( M&& h &ӷ M&r% h % .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &#( +142 h ( M&x& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &a1 +150 h 1 M&y% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&)'o +21 h 'o M&^& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(h% h %3 ."downElevator"\SE.seek :down A('X +100 (+% h % M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +42 h 'X ."downElevator"\SE.coast A(5 +47 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'" @ +19 h @ .t\CS.seek 10ml C':' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M& 1 +2 h 1 M&@& h & M&G% h %\  .t\Elbow.seek FlushPuck::Garage M&7(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&L/ +150 h / M&j& h &- M&`% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C',(W +416 h (W C'k& h &W .tHand.open M&}1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%\ +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%*' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%T' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'_  h +75 .t\PSV.dial :mixloop P%U +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%>  h .t\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial :air P%[  +38 h  .t\PS.seek 2.5ml P%0' +2609 h ' .t\PSV.dial :mixloop P%u +38 h .t\PS.seek 0ml P%W' +2610 h ' .t\PSV.dial :air P%J  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%& +38 h .t\PS.seek 0ml P%=' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&w/ +150 h / .t\CC.seek :open C'!( +416 h ( M&D& h &ӷ M&e% h % .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +264 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&B1 +150 h 1 M&2% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'H& h & M&b% h %\  A(c% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (<( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&$& h &- M&p% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Q( +166 h ( M&z& h & A(A& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &(' +90 h ' A( & h &  .t\SE.seek 7.71 Pucks below Top Plate A(l' +184 h ' A(& h &  M&|& h & .t\Elbow.seek 2 M& ( +7 h ( M&'& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&3'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down M&& A(\\'X h & M&-% h %8 .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +90 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&51 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'v  +151 h  .t\CSV.dial CTV C'd  +19 h  .t\CS.seek 5ml C';'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'X' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%r  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%Y  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%a  +150 h  .t\PS.seek :empty P%y' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'^  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'h  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C's ` +19 h ` .t\CSV.dial :air C'# @ +38 h @ .t\CS.seek 10ml C'+' +2647 h ' .t\CSV.dial CTV C'K  +38 h  .t\CS.seek 0ml C'"'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'R& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M& % h %\  A(@% h %X A(G. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b ()& h &( M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &4( +142 h ( M&/& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(i& h &( .t\SE.seek 11.71 Pucks below Top Plate A('Ɛ +145 h 'Ɛ M&L& h & A(j& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &`'C +25 h 'C .t\Elbow.hold M&{, +7 h , .t\Elbow.reconfigure TightElbow A(n- h -+Ɛ M&$&.,(\ #b .t\SE.seek 12.04 Pucks below Top Plate A(M'3 +27 h '3 M&g% h %C A(7& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&6'o +21 h 'o M&& h & A(k& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &}' +22 h ' .t\SE.seek 13.04 Pucks below Top Plate A(F'" +37 h '" M&\ & h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&1(ӷ +142 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'_( +416 h ( A(U& h &( A(>& h &( M&T& h &ӷ M&[% h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &u( +143 h ( M&0& h & A(J& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &W'C +92 h 'C .t\Elbow.hold M&&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&q% h %C A(o& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&]'o +21 h 'o M&w& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(,% h %" ."downElevator"\SE.seek :down A(D'X +100 (e% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +33 h 'X ."downElevator"\SE.coast A(5 +57 h '\  .t\SC.coast .\PSV.dial :air A(6 P%9  +112 h  P%8% h % .t\PS.seek 0.499ml P%B' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%2 +38 h .t\PRV.dial :air P%.  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%H  +38 h .t\CBV.dial AIV C'b  +38 h .t\PRV.dial between CBV and air P%c  +19 h  .t\PRV.dial :air P%  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %$' +251 h ' .t\PRV.dial :waste2 P%p @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %O' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'z& h & .tHand.open M&A1 +2 h 1 M& & h & M&t% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&|& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&V& h &- M&% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +264 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'l& h &W .tHand.open M& 1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  C'!& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%5 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%v  +76 h  .tdelay 2 seconds +200 P%d% h % .t\PS.seek 1.499ml P%P' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%<' +1094 h ' .t\PSV.dial :air P%:  +38 h  .t\PS.seek 0ml P%;' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%X +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%r  +38 h  .tdelay 2 seconds +200 P%=% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%Y  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%a' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%x  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%^  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %h' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%s +75 h .t\PTV.dial :PRV,avoiding: :puck P%# @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%K  +75 h  .t\PS.seek 2.5ml P%+' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%R  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%@ +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%G  +75 h P%)% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +7 P%/% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'i { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%j' +2610 h ' .t\PSV.dial :PML P%n +37 h .t\PS.seek 0ml P%g' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '7& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Z& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T .t\AWV.select :air .\AIV.dial :bypass F*k  .t\PSV.dial PSR P%\ +21 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*4 I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *`'6 +66 .t\PSV.dial :waste1 P%*  +38 h  .t\PS.seek 0ml P%N' +1597 h ' .t\PSV.dial PSR P%6 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%M' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%{' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*U'ͯ +92 h P%>% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%T' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*[ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%J  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*1  +21 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *q' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%o +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%w' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*,% h % ."MFBPCR"\AS.seek 0.469ml F*S' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%u +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%e' +2610 h ' .t\PSV.dial PSR P% +113 h P%W% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%]' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%F  +38 h  .t\PS.seek 0ml P%9' +739 h ' ."MFBPCR"delay 5 seconds +500 F*89 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*D% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%2' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%. +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%H' +2609 h ' .t\PSV.dial PSR P%b +113 h P%c% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%B' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%p +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%$' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2679 h 'k .t\PSV.dial :waste1 P%t  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %|' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*' I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *&'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%V' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %(' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*?  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *-'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*_ I +43 h I F*l% h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +171 h ' .t\PSV.dial :mixloop P%\\ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %!' +619 h ' ."MFBPCR"\ATV.dial :waste F*5 n +128 h n ."MFBPCR"\AS.seek :empty F*I'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*v% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *d' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*O  +43 h F*P% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *:'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'<  +37 h  C'X% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'r'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'=' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C';  'Y  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*x  +22 h ."MFBPCR"delay 3 seconds +300 F*a% h %_ ."MFBPCR"\AS.seek 0.434ml F*^'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C's'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*#  +22 h F*h% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*K'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*+'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'R' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'@'4 +2647 h '4 C'G& h &W #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&i/ +150 h / .t\CC.seek :open C'( +416 h ( M&L& h &ӷ M&% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &j(- +264 h (- M&7& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'4& h & M&% h %\  A(*% h %X A(E. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (6& h &  M&N& h &- M&}% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &M( +166 h ( M&& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(>& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(`' +195 h ' A({& h &  M&[& h & ."WCR"\Elbow.seek 2 M&U( +7 h ( M&J& h & A(1& h &  ."WCR"\Forearm.seek 2 + 300 counts M& '8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &T/ +59 h '&0 ."MFBPCR"delay 3 seconds +91 h / ."downElevator"\SE.seek :down A(0'X M&o& h & M&% h %8 ."WCR"\Forearm.seek :retract M&,'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +13 ."MFBPCR"\ATV.dial :waste2 F*q % +24 h (ӷ M&u& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +19 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +72 h ' ."WCR"\CC.seek :closed C'e(W +416 h (W ."WCR"Hand.open M&F1 +71 h 'R ."MFBPCR"\ATV.dial :air2 F*]  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *8' +57 h 1 M&~% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)S+ +7 h + S)y% h % C'9& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'.  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %  ."MFBPCR"\AS.seek 0.604ml F*j'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*[% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*J' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*1 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*g  +43 h  ."MFBPCR"\AS.seek 0ml F*T'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*M % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*u' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*w n +108 h n ."MFBPCR"\ASV.dial :wasteR F*q% h %  ."MFBPCR"\AS.seek 0.09ml F*`' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*U I +86 h I F*0% h % ."MFBPCR"\AS.seek 0.12ml F*,' +625 h ' ."MFBPCR"\ATV.dial :air3 F*+ % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*~% h % ."MFBPCR"\AS.seek 0.23ml F*y' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h % ."MFBPCR"\AS.seek 0.255ml F*.'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*S' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*c n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*b  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*2 @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*A'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*p'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*t  +43 h  #"MFBPCR"\Swish #1 of 2 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *''\  +1032 h '\  F*z% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*$% h %(A ."MFBPCR"\AS.seek 0.6ml F*V'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*%'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*?% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *|' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +3472 h 'V ."MFBPCR"\SPE.coast F*_  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0517h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*l  +64 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*-% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +21 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*d  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*X% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*r  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*Y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*x n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 F*K  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226157 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'G @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'Q @ +76 h @ +50 S)/% h % ."WCR"\CBV.dial between CSV and waste2b C'3  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'm ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'7  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'\  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Z' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'n  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'E' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'4 @ +75 h @ +50 S)*% h % ."WCR"\CBV.dial between CSV and waste2b C'}  +94 h  C'k% h % ."WCR"\CSV.dial between air and CTV C'N ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'>  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '{'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'[  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'j' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'1  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'g  +38 h  C'J% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'T' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'M @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'w% h % ."WCR"\CSV.dial between air and CTV C'q ` +57 h ` ."WCR"\CS.seek 7.288ml C'u' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'U  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'`  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '+' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C',& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&F% h %\  A(~% h %X A(]. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (9(( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (W& h &( M&y& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&.& h & A(c& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &b' +90 h ' A(S& h &( ."WCR"\SE.seek 12.71 Pucks below Top Plate A(' +136 h ' M&H& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &'C +25 h 'C ."WCR"\Elbow.hold M&B, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(A- h -+ M&$&.,(\ #b ."WCR"\SE.seek 13.04 Pucks below Top Plate A(t'" +27 h '" M& % h %C A(p& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&z'o +21 h 'o M&'& h & A($& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 14.04 Pucks below Top Plate A(' +36 h ' M&V& h & M&?% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&|& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ ."WCR"\Forearm.seek CC M&l' +91 h ' ."WCR"Hand.close M&\\/ +150 h / ."WCR"\CC.seek :open C'-( +416 h ( A(&& h &( A(& h &( M&8& h &ӷ M&5% h % ."WCR"\Forearm.seek :retract M& '\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b & ( +142 h ( M&O& h & A(P& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &v'C +93 h 'C ."WCR"\Elbow.hold M&d, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &X1 +150 h 1 M&I% h %C A(r& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&;'o +21 h 'o M&<& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &=' +22 h ' A("% h % ."downElevator"\SE.seek :down A(a'X +100 (% h %v M&Y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&h& h & M&#% h %\  .t\Elbow.seek FlushPuck::Garage M&:(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'/(W +416 h (W C'3& h &W .tHand.open M&L1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'7& h &W C'% h % .t\CSV.dial CSR C'  +38 h  C'\ % h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'E& h &W C'4% h %4 .t\CSV.dial :flush C'*  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'N% h %p .t\CS.seek 7ml C'6'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '>  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'['4 +766 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C'1% h %4 .t\CSV.dial :flush C'g  +76 h  .t\CS.seek 5ml C'J'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +38 h @ C'T% h %p .t\CS.seek 7ml C'M'i +415 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'w'4 +766 h '4 #t\Flushing upper manifold C'q& h &W C'o% h %4 .t\CSV.dial :flush C'U  +76 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'`% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  ', +37 h +75 .t\CTV.dial :air C'F  +151 h .t\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C'W +37 h .t\CS.seek 0ml C'y'4 +507 h '4 C'& h &W C'.% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.seek 3ml C'S'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'H% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'A'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'p +76 h .t\CS.seek 0ml C'D'4 +506 h '4 #tDisinfecting external Intake with mfbkill C''& h &W C'$% h %4 .t\CSV.dial CSR C'V  +151 h  C'?% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'b'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +415 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'|' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'_  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'z'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C't  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'B'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +709 h ' ."connect"\CSV.dial CTV C'%  .t\CTV.dial CSV C'e  +38 h  +113  .t\CBV.dial :waste1 C'& @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'8  +38 h  C'5% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'O'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +709 h ' ."connect"\CSV.dial CTV C'l  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'X'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'I  +38 h  C'r% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  '" +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'h& h &W C'#% h %4 .t\CSV.dial :flush C'v  +38 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'^% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'= +112 h .t\CTV.dial :air .\CS.seek 0ml C'a'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +507 h '0 .t\CSV.dial :waste C'd +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C's% h %4 .t\CSV.dial :flush C'x  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'@% h %0 .t\CS.seek 5ml C':'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C')'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'L'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'm& h &W C'7% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'Q% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'E& h &W C'4% h %4 .t\CSV.dial :flush C'*  +76 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'N% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV C'>  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tDisinfecting external Intake with kill C'6& h &W C'{% h %4 .t\CSV.dial CSR C'1  +38 h  C'g% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'['L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'j' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'J 'T  +37 h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'M  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'q'4 +606 h '4 .t\CSV.dial CSR C'o  +113 h  C'U% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'w'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'u' +1372 h ' .t\CSV.dial CBV C'` +113 h .t\CS.seek 0ml C' '4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +6 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C']( +416 h ( M&& h &ӷ M&.% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &y(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &S'η +91 h 'η .tHand.open M&1 +150 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&'1 +2 h 1 M&$& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &V(- +7 h (- M&?& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.seek :garage M&b'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +264 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +91 h ' .t\CC.seek :closed C'B(W +416 h (W C'%& h &W .tHand.open M&e1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'8& h &W C'5% h %4 .t\CSV.dial CSR C'  +113 h  C'O% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'P'[ +462 h '[ .t\CSV.dial :waste C'I +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'r'4 +2010 h '4 #t\Purging external Intake C'X& h &W C'<% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'Y& h &W C'h% h %4 .t\CSV.dial :flush C'#  +76 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +112 h .t\CTV.dial :air .\CS.seek 0ml C'='4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C'a +38 h .t\CS.seek 0ml C'd'4 +506 h '4 C'9& h &W C'% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C'x'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'G% h %0 .t\CS.seek 5ml C'@'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +507 h '0 .t\CSV.dial :waste C') +75 h .t\CS.seek 0ml C'3'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&-& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'Q( +416 h ( M&& h &ӷ M&n% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &4'η +91 h 'η .tHand.open M&*1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST06-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:54:30CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST06-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0517h1500ml.pcr F*N% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*.'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0519h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*V'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*] n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +21 h ."MFBPCR"delay 2 seconds +200 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*O  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*z% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.53ml F*I' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*P  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.536ml F*<'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*r% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*v'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*^ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0519h1500ml.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*K  +22 h ."MFBPCR"\ATV.dial :air3 F*9 % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *s'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +21 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*7  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*\ % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *|'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +31 h  ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*4% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0521h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*U  +10 h  F*q% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*u n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*W  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b */'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*~' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*,' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*c'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*''\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*$ n +21 h n ."MFBPCR"delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*S  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*t'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0521h1500ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*_  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +87 h % F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +21 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*^'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*v'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*9% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V @CEST1538776816.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0600h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*-  +11 h  F*|% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*m  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*4% h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*N n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*>'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +108 h  ."MFBPCR"delay 7 seconds +700 F*Z% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*T  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0600h1500ml.pcr F*o% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *w'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*u  +22 h ."MFBPCR"\ATV.dial :air3 F*` % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*,% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*%% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0602h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*P  +11 h  F*r% h %V ."MFBPCR"\AS.seek 0.5ml F*<'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*" n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*# I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*;% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*2 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.53ml F*^' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*K  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F*v'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*a  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*d'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*x'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*@  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*:  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*7% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0602h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *\\  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*B  +151 h F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *E' +423 h ' ."MFBPCR"\ATV.dial :ASV F*m n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F**  +43 h  F*L% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*4 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *+'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *N'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*R'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *}' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +21 h F*>% h % ."MFBPCR"\AS.seek 0.71ml F*6' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h % H ."MFBPCR"\AS.seek 0.735ml F*g' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*[% h % Ű ."MFBPCR"\AS.seek 0.72ml F*T' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*M n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*U n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*o % +43 h % F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*w' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*i% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*.  +22 h  ."MFBPCR"\AS.seek 0ml F*/'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *H'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*, % +108 h % F*~% h %V ."MFBPCR"\AS.seek 0.01ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*c% h % ."MFBPCR"\AS.seek 0.58ml F*$' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*S' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*b n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*y'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *V'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *_' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*% n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *t'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*] n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +202 h 'R ."MFBPCR"\SPE.coast F*5  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*8 n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*z  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *O' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*l I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*X  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *P': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*r  +64 h  F*I% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *<'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*" n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Y  +43 h  F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *;': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*h  +65 h  F*2% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *='Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*K% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *9' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*^ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *v': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *s'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*d n +151 h n ."MFBPCR"\AS.seek 0ml F*@'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*G  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*x % +43 h % F*:% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*)% h % ."MFBPCR"\AS.seek 0.96ml F*-'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %0 ."MFBPCR"\AS.seek 0.985ml F*\ ' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*n% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*m I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *4'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *+'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*N  +43 h  F*f% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *R' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*}' +9880 h ' ."MFBPCR"\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.seek 0ml F*>'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*{% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*1  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*M% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*u% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*.  +32 h  ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +21 h F*~% h % ."MFBPCR"\AS.seek 0.81ml F*H'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*'% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *_'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *t'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*& % +108 h % ."MFBPCR"\AS.seek 0ml F*8'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast .\CS.coast S)5 C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )z h  C' ' h  P% %e h  F* *l h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *O  h  .t\SPE.configure SPEconfig F*\ 2 * % h %V .t\AS.configure ASconfig S)X% h % F*#,,'T(^20( b .t\SS.configure SSconfig C' & S)#.0X 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:55:29CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #6) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&\ 1 +150 h 1 M&m& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &*(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &4'η +91 h 'η .tHand.close M& / +150 h / M&N& h &- M&f% h %η .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +91 h ' .t\CC.seek :closed C'{(W +416 h (W C'}& h &W .tHand.open M&>1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'J& h &W C'% h %4 .t\CSV.dial CSR C'g  +150 h  C'M% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '['p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'T% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'U'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'o'4 +2009 h '4 #t\Purging external Intake C'j& h &W C'% h %4 .t\CSV.dial :flush C'`  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u 'q  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'F'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'w  +75 h #t\Flushing upper manifold C'& h &W C'i% h %4 .t\CSV.dial :flush C'W  +75 h  .t\CS.seek 5ml C'.'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'/% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', +113 h .t\CTV.dial :air .\CS.seek 0ml C'~'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'c'4 +507 h '4 C'H& h &W C'?% h %4 .t\CSV.dial :flush C''  +38 h  .t\CS.seek 3ml C'A'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +38 h @ C'$% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'S'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &p(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'5( +416 h ( M&]& h &ӷ M&&% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&C1 +150 h 1 M&!% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:55:35CEST30-Sep-18\\n{1 => 15, 2 => 7, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:55:29CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*Q% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&z% h %\  A(% h %X A(e. h ., A(l& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (O& h &  M& & h &- M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*r % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *I' +160 h ( M&P& h & A("& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(<& h &  .t\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' A( & h &  M&& h & .t\Elbow.seek 2 M&Y( +7 h ( M&#& h & A(h& h &  .t\Forearm.seek 2 + 300 counts M&2'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(K'X h & M&=% h %8 .t\Forearm.seek :retract M&9'\ +91 h '\  .t\Elbow.seek CC M&d(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'|(W +415 h (W .tHand.open M&\\1 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)x+ +7 h + S)@% h % C'n& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X+ +6 h + S)1% h % C'J& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)  +76 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'g% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Pd +100 C'Z9 h 9Td +100 C' 9 h 9Xd +100 C'T9 h 9(\\d +100 C'[9 h 9@`d +100 C'9 h 9Wdd +100 C'U9 h 9nhd +100 C'j9 h 9ld +100 C'9 h 9pd +100 C'`9 h 9td +100 C'M9 h 9xd +100 C'q9 h 9|d +100 C'u9 h 9d +100 C'w9 h 9d +100 C'9 h 9'd +100 C'S9 h 9>d +100 C'i9 h 9Td +100 C'9 h 9kd +100 C'W9 h 9d +100 C'F9 h 9d +100 C'/9 h 9d +100 C'.9 h 9d +100 C',9 h 9d +100 C'9 h 9d +100 C'~9 h 9 d +100 C'o9 h 9 d +100 C'9 h 96d +100 C'9 h 9Ld +100 C'H9 h 9bd +100 C'?9 h 9xd +100 C'09 h 9d +100 C'c9 h 9d +100 C'D9 h 9d +100 C'$9 h 9d +100 C''9 h 9d +100 C'A9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'y9 h 9>d +100 C'9 h 9Td +100 C'V9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'b9 h 9d +100 C'_9 h 9d +100 C'69 h 9d +100 C'{9 h 9d +100 C'&9 h 9  d +100 C'(9 h 9 d +100 C'9 h 9 *d +100 C'%9 h 9 ?d +100 C'k9 h 9 Td +100 C'C9 h 9 j d +100 C']9 h 9 $d +100 C'p9 h 9 (d +100 C't9 h 9 ,d +100 C'Q9 h 9 0d +100 C'z9 h 9 4d +100 C'9 h 9 8d +100 C'e9 h 9 (( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (Z& h &( M& & h &ӷ M&T% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &[( +142 h ( M&& h & A(U& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &j' +90 h ' A(& h &( .t\SE.seek 13.71 Pucks below Top Plate A(`'n +125 h 'n M&M& h & A(q& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &u'C +25 h 'C .t\Elbow.hold M&w, +7 h , .t\Elbow.reconfigure TightElbow A(- h -+n M&$&.,(\ #b .t\SE.seek 14.04 Pucks below Top Plate A(S' +27 h ' M&i% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&W'o +21 h 'o M&F& h & A(/& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &.' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(,' +36 h ' M&& h & M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.seek CC M&?' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'c( +416 h ( A(D& h &( A($& h &( M&'& h &ӷ M&A% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&y& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &V'C +93 h 'C .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&b% h %C A(_& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &6'o +21 h 'o M&{& h & A(&& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &(' +22 h ' A(% h % ."downElevator"\SE.seek :down A(%'X +100 (k% h %- M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'p @ +19 h @ .t\CS.seek 10ml C't' +7 h ' .t\CSV.dial between air and CTV C'Q ` +18 h ` .t\AIV.dial :bypass C'z { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&e1 +2 h 1 M&l& h & M&O% h %\  .t\Elbow.seek FlushPuck::Garage M&!(- +166 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&/ +150 h / M&"& h &- M&8% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &3' +91 h ' .t\CC.seek :closed C'h(W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%# +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%=' +1597 h ' .t\PSV.dial PSR P%7 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'2  P%  h +75 .t\PSV.dial :mixloop P%Y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%9  h .t\PS.seek 0ml P%K' +2610 h ' .t\PSV.dial :air P%\\  +38 h  .t\PS.seek 2.5ml P%5' +2609 h ' .t\PSV.dial :mixloop P%@ +38 h .t\PS.seek 0ml P%n' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%x' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.seek CC M&\ ' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'^( +416 h ( M&f& h &ӷ M&)% h % .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &I(- +264 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&B1 +150 h 1 M&m% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'1& h & M&J% h %\  A(% h %X A(g. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (}( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (Z& h &  M& & h &- M&T% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&U& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &d' +90 h ' A(M& h &  .t\SE.seek 4.71 Pucks below Top Plate A(q' +214 h ' A(& h &  M&i& h & .t\Elbow.seek 2 M&( +7 h ( M&F& h & A(/& h &  .t\Forearm.seek 2 + 300 counts M&v'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(~'X M&j& h & M&[% h %8 .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.seek CC M&u(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +19 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &W' +91 h ' .t\CC.seek :closed C'S(W +416 h (W .tHand.open M&.1 +150 h 1 M&w% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'o  +151 h  .t\CSV.dial CTV C'|  +18 h  .t\CS.seek 5ml C'D'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'$' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%A  +38 h  .t\PS.seek 2.5ml P%y' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%?  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%b' +2610 h ' .t\CBV.dial :waste1 C'_ @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'{  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'& @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'k' +2647 h ' .t\CSV.dial CTV C'C  +37 h  .t\CS.seek 0ml C'p'4 +2648 h '4 .t\CBV.dial :waste1 C'Q @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'z  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&e% h %\  A(l% h %X A(O. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (V(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (6& h &( M&,& h &ӷ M&(% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &X( +143 h ( M&& h & A(%& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +89 h ' A(4& h &( .t\SE.seek 14.71 Pucks below Top Plate A( '] +116 h '] M&& h & A("& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &8'C +25 h 'C .t\Elbow.hold M&], +7 h , .t\Elbow.reconfigure TightElbow A(r- M&$&.,(\ #b h -+] .t\SE.seek 15.04 Pucks below Top Plate A(' +27 h ' M&% h %C A(!& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&<'o +21 h 'o M&c& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 16.04 Pucks below Top Plate A( ' +37 h ' M&#& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&=' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'2( +416 h ( A(Y& h &( A(9& h &( M&& h &ӷ M&\\% h % .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &@( +143 h ( M&5& h & A(;& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &n'C +92 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&G% h %C A(L& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&E'o +21 h 'o M&N& h & A(P& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &s' +22 h ' A(h% h % ."downElevator"\SE.seek :down A(f'X +100 ()% h %X M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast .\PSV.dial :air P%*  A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%B' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%m +38 h .t\PRV.dial :air P%:  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%1  +38 h .t\CBV.dial AIV C'J  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%g  +18 h  P%Z% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +251 h ' .t\PRV.dial :waste2 P%T @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'U& h & .tHand.open M&1 +2 h 1 M&M& h & M&I% h %\  .t\Elbow.seek FlushPuck::Garage M&+(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.seek :garage M&F'η +91 h 'η .tHand.close M&// +150 h / M&& h &- M&j% h %η .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &H' +91 h ' .t\CC.seek :closed C'v(W +416 h (W C'q& h &W .tHand.open M&1 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  C'^& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%. @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%w  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%o  +76 h  .tdelay 2 seconds +200 P%|% h % .t\PS.seek 1.499ml P%W' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%}' +1094 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 0ml P%D' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%$ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%A  +38 h  .tdelay 2 seconds +200 P%x% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%?  +76 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%y  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%_'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%{  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%&  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%'' +2610 h ' .t\PSV.dial :air P%C  +75 h  .t\PS.seek 2.5ml P%k' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%z' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%e' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%l +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%O  +75 h P%6% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %,' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'( & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %%'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'4 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %u' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :PML P%r +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '!& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'c& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *0  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%# +21 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*X I +129 h I F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *8'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%7' +1597 h ' .t\PSV.dial PSR P%< +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%]' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%3 +21 h  F*S% h %6 ."MFBPCR"\AS.seek 0.329ml F*Y'ͯ +92 h P%9% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*\\ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +37 h  .t\PS.seek 0ml P%=' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *G' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%L +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%N' +217 ."MFBPCR"\ATV.dial :AIV F*P I +43 h I F*h% h % ."MFBPCR"\AS.seek 0.469ml F*K' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%@ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%)' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%n% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%E' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%*' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*s n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f% h % ."MFBPCR"\AS.seek 0.041ml F*V'% +336 h ' .t\PSV.dial PSR P%b +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%m' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%1' +2609 h ' .t\PSV.dial PSR P%J +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%Z  +38 h  .t\PS.seek 0ml P%B' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%T +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %M'k +2679 h 'k .t\PSV.dial :waste1 P%I  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*i I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%/  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %R' +5106 h ' .t\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %d' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *['Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*2 I +43 h I F*q% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%~ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %^' +619 h ' ."MFBPCR"\ATV.dial :waste F*. n +128 h n ."MFBPCR"\AS.seek :empty F*H'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*w  +86 h  F*o% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *|' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*W% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'}  +37 h  C'$% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'A'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'x' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  '?  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*y  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*{'ݏ +110 h '4 .t\CSV.dial CSR C'&  +37 h  C'_% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*C'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*' n +107 h n F*Q% h %& ."MFBPCR"\AS.seek 0.092ml F*k'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'z  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'l'4 +2647 h '4 C'O& h &W #tGaraging FlushPuck in Collection Clamp M&6& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &e(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&,' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'%( +416 h ( M&& h &ӷ M&u% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &"(- +264 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'X& h & M& % h %\  A( % h %X A(a. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (>( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (<& h &  M&7& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(S& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &3' +90 h ' A(9& h &  ."WCR"\SE.seek 3.71 Pucks below Top Plate A(8' +223 h ' A(]& h &  M&\\& h & ."WCR"\Elbow.seek 2 M&Y( +7 h ( M&;& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +31 h '&0 ."MFBPCR"delay 3 seconds +119 h / ."downElevator"\SE.seek :down M&L& A(5'X h & M&P% h %8 ."WCR"\Forearm.seek :retract M&h'\ +91 h '\  ."WCR"\Elbow.seek CC M&=(ӷ +90 ."MFBPCR"\ATV.dial :waste2 F*G % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R h 'X ."downElevator"\SE.coast A(5 +9 h (ӷ M&N& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' ."WCR"\CC.seek :closed C')(W +416 h (W ."WCR"Hand.open M& 1 +43 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +85 h 1 M&s% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)K+ +7 h + S)b% h % C'*& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C':  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*K' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*J  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *g'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*m @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*V I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*T'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*I  +43 h  #"MFBPCR"\Swish #1 of 2 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *i'\  +1032 h '\  F*U% h %\  ."MFBPCR"\AS.seek 0.5ml F*/'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*F% h %\  ."MFBPCR"\AS.seek 0.5ml F*R'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*j % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*2  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0617h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*q  +64 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*[% h %V ."MFBPCR"\AS.seek 0.5ml F*+'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *H'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*|  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*}' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*A  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*p' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*x% h % ."MFBPCR"\AS.seek 0.536ml F*?'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*&% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*t'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*'' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*{  +43 h  ."MFBPCR"\ASV.dial :waste1 F*C  +65 h ."MFBPCR"\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226185 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'(  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'O @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '4' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'v  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'` ` +19 h ` ."WCR"\CS.seek 7.288ml C',' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'!  'u  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'e'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'#  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'c' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'r  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'a' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'X @ +75 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'0% h % ."WCR"\CSV.dial between air and CTV C'7 ` +57 h ` ."WCR"\CS.seek 7.288ml C'S' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'9  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C']  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '"' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C';  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'\\% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)3% h % ."WCR"\CBV.dial between CSV and waste2b C'P  +95 h  C'N% h % ."WCR"\CSV.dial between air and CTV C'\ ` +56 h ` ."WCR"\CS.seek 7.288ml C'G' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'8  'Y  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'k'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'h' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'%  +151 h C'5& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M& % h %\  A(s% h %X A(E. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (*(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (@& h &( M&b& h &ӷ M&n% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &>( +142 h ( M&:& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &J' +90 h ' A(K& h &( ."WCR"\SE.seek 15.71 Pucks below Top Plate A(Z'L +106 h 'L M&1& h & A(g& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &V'C +26 h 'C ."WCR"\Elbow.hold M&B, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+L ."WCR"\SE.seek 16.04 Pucks below Top Plate A(I' +27 h ' M&M% h %C A(T& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&U'o +20 h 'o M&i& h & A( & h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &/' +22 h ' ."WCR"\SE.seek 17.04 Pucks below Top Plate A(F' +37 h ' M&& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +89 h '\  ."WCR"\Elbow.seek CC M&j(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ ."WCR"\Forearm.seek CC M&q' +91 h ' ."WCR"Hand.close M&~/ +150 h / ."WCR"\CC.seek :open C'[( +415 h ( A(& h &( A(+& h &( M&& h &ӷ M&.% h % ."WCR"\Forearm.seek :retract M&w'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&& h & A(W& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &o'C +92 h 'C ."WCR"\Elbow.hold M&|, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &$1 +150 h 1 M&H% h %C A(A& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&D'o +20 h 'o M&}& h & A(& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &x' +22 h ' A(p% h % ."downElevator"\SE.seek :down A('X +93 h 'X ."downElevator"\SE.coast A(5 +7 (&% h %X M&?% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +90 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&_1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&t(- +166 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&Q'η +91 h 'η .tHand.close M&z/ +150 h / M&'& h &- M&% h %η .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'v& h &W .tHand.open M&1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'u& h &W C'!% h % .t\CSV.dial CSR C',  +37 h  C'#% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'e'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'c'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'X% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'7% h %p .t\CS.seek 7ml C'<'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '9  +38 h  +112  .t\CBV.dial :waste1 C'S @ +75 h @ .t\CS.seek 0ml C']'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C';% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'3  +38 h  .t\CBV.dial :waste2b C'P  +75 h  .t\CS.seek 0ml C'N'4 +766 h '4 #t\Flushing upper manifold C'\ & h &W C'L% h %4 .t\CSV.dial :flush C'Y  +75 h  .t\CS.seek 5ml C'8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'% h %p .t\CS.seek 7ml C'k'i +416 h 'i ."connect"\CSV.dial CBV C'% .t\CBV.dial CSV C'5  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C's'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +507 h '0 .t\CSV.dial :waste C'@ +38 h .t\CS.seek 0ml C'b'4 +506 h '4 C'n& h &W C':% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'K'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +38 h @ C'1% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +507 h '0 .t\CSV.dial :waste C'T +75 h .t\CS.seek 0ml C'i'4 +507 h '4 #tDisinfecting external Intake with mfbkill C' & h &W C'f% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'J'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'>' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z +113 h .t\CTV.dial :intake C'V  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'2  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'U'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'I  +113 h  C'/% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'B'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +710 h ' ."connect"\CSV.dial CTV C'R  .t\CTV.dial CSV C')  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'+'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'.% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'q  +37 h  .t\CBV.dial :waste2b C'j  +76 h  .t\CS.seek 0ml C'$'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'H  +38 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'p 'w  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'&'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'_'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'o  +38 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +38 h @ C'{% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'x'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'*'0 +506 h '0 .t\CSV.dial :waste C'| +38 h .t\CS.seek 0ml C'z'4 +507 h '4 C''& h &W C'% h %4 .t\CSV.dial :flush C'y  +37 h  .t\CS.seek 3ml C'Q'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +37 h @ C'l% h %0 .t\CS.seek 5ml C't'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +506 h '0 .t\CSV.dial :waste C'v +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'`& h &W C'u% h %4 .t\CSV.dial :flush C'!  +38 h  .t\CS.seek 5ml C','p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +38 h @ C'% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CTV C'r  .t\CTV.dial CSV C'e  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'a& h &W C'X% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'7% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'9  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'S'4 +766 h '4 #tDisinfecting external Intake with kill C'<& h &W C'% h %4 .t\CSV.dial CSR C';  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C']'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'"' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'\\  +38 h +75 .t\CTV.dial :intake C'3  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'P' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '\ '4 +607 h '4 .t\CSV.dial CSR C'L  +113 h  C'Y% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'N'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'G' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'8'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&5& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'E( +415 h ( M&n& h &ӷ M&:% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &b(- +265 h (- M&=& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &K'η +91 h 'η .tHand.open M&g1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M& 1 +2 h 1 M&f& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.seek :garage M&J'η +91 h 'η .tHand.close M&Z/ +150 h / M&V& h &- M&>% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &U' +91 h ' .t\CC.seek :closed C'B(W +416 h (W C'R& h &W .tHand.open M&)1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'.% h %4 .t\CSV.dial CSR C'+  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'q'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'W'[ +463 h '[ .t\CSV.dial :waste C'H +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'A'4 +2010 h '4 #t\Purging external Intake C'$& h &W C'}% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'p' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'&'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'?& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'o'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C'F% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CBV C'D .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +506 h '0 .t\CSV.dial :waste C'x +38 h .t\CS.seek 0ml C'|'4 +507 h '4 C'*& h &W C''% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'y'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +37 h @ C'O% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Q'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +506 h '0 .t\CSV.dial :waste C'6 +76 h .t\CS.seek 0ml C'v'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +7 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'( +416 h ( M&,& h &ӷ M&r% h % .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &X'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST07-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:57:17CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST07-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0617h1500ml.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*9  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*]% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*P  +64 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*(% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *4' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*n% h % ."MFBPCR"\AS.seek 0.81ml F*:'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*1'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*g% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0619h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +11 h  F*/% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*8' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*j  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*H' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*W  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*}'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*w  +108 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*_  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*o'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*{ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0619h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*C  +22 h ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*z% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +21 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +21 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +65 h  F*#% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +31 h  ."MFBPCR"\ATV.dial :air3 F*` % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0621h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*Y  +10 h  F*\ % h %V ."MFBPCR"\AS.seek 0.5ml F*N'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*@  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*n n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*4% h %Fj ."MFBPCR"\AS.seek 0.524ml F*s' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*= I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.53ml F*%' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*g % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*1% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*M% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*i'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*K  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*V' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*>  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*b  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*J% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0621h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*2  +22 h ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +21 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*w% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*& % +87 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*D% h % ."MFBPCR"\AS.seek 0.81ml F*{'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F**% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*x  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V @CEST1538863216.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0700h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ARV.dial :carrier F*[  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*, n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*a  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*`  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*e I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*X% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*7 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*9'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*c% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*< n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*]'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*P% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Y  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0700h1500ml.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*G  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *@'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +64 h  F*%% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*V% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0702h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ARV.dial :carrier F*W  +11 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*p n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*D% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*m % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*o'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*x  +22 h  ."MFBPCR"delay 7 seconds +700 F*'% h %< ."MFBPCR"\AS.seek 0.594ml F*|'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*z n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*6' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*u% h % \\ ."MFBPCR"\AS.seek 0.326ml F*#'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0702h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*! @@T ."MFBPCR"\ASV.dial :SPEdelivery F*~  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*B  +151 h F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *a' +423 h ' ."MFBPCR"\ATV.dial :ASV F*` n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*e'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*X n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *7'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*9% h % ."MFBPCR"\AS.seek 0.71ml F*<' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*]% h % Ű ."MFBPCR"\AS.seek 0.72ml F*\\' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*P% h % zG ."MFBPCR"\AS.seek 0.365ml F*3' +7161 h ' ."MFBPCR"\ATV.dial :waste F*Y n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*L % +43 h % F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *G' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*5% h % ."MFBPCR"\AS.seek 0.579ml F*N' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*@'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*4% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*= n +86 h n ."MFBPCR"\ASV.dial :wasteR F*:  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *1'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*% % +108 h % F*s% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*f' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % q ."MFBPCR"\AS.seek 0.605ml F*Z'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +21 h F*>% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*K' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*J n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*V  +21 h  ."MFBPCR"\AS.seek 0ml F*b'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*/  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *2' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*R n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *I'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*E n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*.  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*U  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *W': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*A  +64 h  F*H% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *}'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*p n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*?  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *D': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*m% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*F n +65 h n F*C% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b **' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*{ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *o': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*z n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*| n +151 h n ."MFBPCR"\AS.seek 0ml F*l'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*O  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*y % +43 h % F*t% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*6% h % ."MFBPCR"\AS.seek 0.96ml F*['0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %0 ."MFBPCR"\AS.seek 0.985ml F*#' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*r% h % ."MFBPCR"\AS.seek 0.97ml F*,'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*` I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *X'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*7  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*c n +129 h n ."MFBPCR"\AS.seek 0ml F*9'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*P'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*3'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*5  +64 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *g' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*1'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *>'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*. n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *I'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )U h  C' '+ h  P% %) h  F* *q h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ C' 2\ F*\ 9\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *8% h %V .t\AS.configure ASconfig S)$% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'w& h & .t\CC.configure CCconfig S)#.0X 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:58:17CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #7) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'r& h & .tHand.open M +150 h 1 M&`& h &M M&,% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &X'η +91 h 'η .tHand.close M&e/ +150 h / M&7& h &- M&% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&91 +150 h 1 M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'S% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '\\% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Y'[ +463 h '[ .t\CSV.dial :waste C'P +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'"& h &W C'3% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'G '\  h +75 .t\CTV.dial :intake C'(  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'N  +75 h #t\Flushing upper manifold C'n& h &W C'4% h %4 .t\CSV.dial :flush C'@  +75 h  .t\CS.seek 5ml C':'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +37 h @ C'% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CBV C'% .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'M +37 h .t\CS.seek 0ml C''4 +507 h '4 C'1& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +38 h @ C'f% h %0 .t\CS.seek 5ml C'>'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'V +75 h .t\CS.seek 0ml C'K'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&b& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +7 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C'.( +416 h ( M&E& h &ӷ M&% h % .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&-1 +150 h 1 M&^% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:58:23CEST30-Sep-18\\n{1 => 18, 2 => 4, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:58:17CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*v% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'!& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&U% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(+% h %X A(). h ., A(q& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&8& h &- M&$% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &w( +6 F*j% h %&K ."readyMFB"\ATV.dial :waste2 F*A % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *H' +160 h ( M&W& h & A(p& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(}& h &  .t\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') A(& h &  M&&& h & .t\Elbow.seek 2 M&?( +7 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&m'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &F/ +150 h / ."downElevator"\SE.seek :down A(C'X M&_& h & M&% h %8 .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.seek CC M&|(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +2 h 'X ."downElevator"\SE.coast A(5 +89 h ' .t\CC.seek :closed C'~(W +416 h (W .tHand.open M&1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)y+ +7 h + S)l% h % C'r& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'5J +216 h '5J ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)Z  +151 h  .t\CTV.dial :air,avoiding: :intake C'V  +150 h ."CSpush"\CSV.dial CTV .t\SS.seek 10ml S)J' +100 ."CSpush"\CSV.dial :air C'b @ +38 h @ ."CSpush"\CS.reconfigure CSfast .\CS.seek 0ml C'#d @b '/'4 +6 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S) +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'49 h 9Td +100 C'(9 h 9kd +100 C'@9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C':9 h 9d +100 C'%9 h 9d +100 C'=9 h 9d +100 C's9 h 9 d +100 C'L9 h 9 d +100 C'n9 h 96d +100 C'g9 h 9Ld +100 C'19 h 9bd +100 C'9 h 9xd +100 C'k9 h 9d +100 C'9 h 9d +100 C'i9 h 9d +100 C'f9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'Z9 h 9d +100 C'V9 h 9(d +100 C'b9 h 9>d +100 C'>9 h 9Td +100 C'9 h 9id +100 C'M9 h 9d +100 C'/9 h 9d +100 C'J9 h 9d +100 C'29 h 9d +100 C'<9 h 9d +100 C'9 h 9d +100 C'9 h 9  d +100 C'd9 h 9 d +100 C'9 h 9 *d +100 C'R9 h 9 ?d +100 C'09 h 9 Td +100 C'-9 h 9 j d +100 C'E9 h 9 $d +100 C'T9 h 9 (d +100 C'I9 h 9 ,d +100 C'v9 h 9 0d +100 C'U9 h 9 4d +100 C'+9 h 9 8d +100 C')9 h 9 & h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&M, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &/1 +150 h 1 M&J% h %C A(2& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&<'o +21 h 'o M&& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(% h % ."downElevator"\SE.seek :down A(R'X +83 h 'X ."downElevator"\SE.coast A(5 +17 (0% h %X M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +89 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'T @ +19 h @ .t\CS.seek 10ml C'I' +7 h ' .t\CSV.dial between air and CTV C'v ` +18 h ` .t\AIV.dial :bypass C'U { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&)1 +2 h 1 M&q& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&^(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.seek :garage M&A'η +91 h 'η .tHand.close M&!/ +150 h / M&p& h &- M&% h %η .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&F1 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%_% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%w' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%u +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%'' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%m  C'  h +75 .t\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%C  h .t\PS.seek 0ml P%*' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%.' +2609 h ' .t\PSV.dial :mixloop P%l +38 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%D  +37 h  .t\PS.seek 2.5ml P%O' +2610 h ' .t\PSV.dial :mixloop P%` +38 h .t\PS.seek 0ml P%y' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'{( +416 h ( M&& h &ӷ M&6% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&[1 +150 h 1 M&,% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C';& h & M&% h %\  A(S% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (c& h &  M&e& h &- M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &P( +166 h ( M&Y& h & A(3& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &|' +90 h ' A(& h &  .t\SE.seek 1.71 Pucks below Top Plate A(\ ': +244 h ': A(5& h &  M&4& h & .t\Elbow.seek 2 M&(( +6 h ( M& & h & A(& h &  .t\Forearm.seek 2 + 300 counts M&o'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &=/ +150 h / ."downElevator"\SE.seek :down M&"& A(s'X h & M&]% h %8 .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&G(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &@' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'K(W +416 h (W .tHand.open M&:1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'L  +150 h  .t\CSV.dial CTV C'~  +19 h  .t\CS.seek 5ml C'i'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'f' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :mixloop P%> +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%n +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%/  +151 h  .t\PS.seek :empty P%J' +2609 h ' .t\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'Z ` +19 h ` .t\CSV.dial :air C'V @ +37 h @ .t\CS.seek 10ml C'0' +2647 h ' .t\CSV.dial CTV C'-  +38 h  .t\CS.seek 0ml C'T'4 +2647 h '4 .t\CBV.dial :waste1 C'v @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'U  +151 h C'+& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&)% h %\  A(q% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (<& h &( M&%& h &ӷ M&R% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &d( +143 h ( M&$& h & A(j& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &9' +89 h ' A(X& h &( .t\SE.seek 17.71 Pucks below Top Plate A(M'* +86 h '* M&!& h & A(p& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &'C +25 h 'C .t\Elbow.hold M&E, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(A- h -+* .t\SE.seek 18.04 Pucks below Top Plate A( ' +27 h ' M&% h %C A(^& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&}'o +21 h 'o M&& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &F' +22 h ' .t\SE.seek 19.04 Pucks below Top Plate A(8' +37 h ' M&& h & M&_% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&m/ +150 h / .t\CC.seek :open C'( +416 h ( A(?& h &( A(C& h &( M&'& h &ӷ M&% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &l( +143 h ( M&.& h & A(D& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &r'C +92 h 'C .t\Elbow.hold M&`, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &O1 +150 h 1 M&z% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&a'o +21 h 'o M&7& h & A(W& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +74 h 'X ."downElevator"\SE.coast A(5 +26 (6% h %X M&#% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +112 h  P%Q% h % .t\PS.seek 0.499ml P%[' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%, +38 h .t\PRV.dial :air P%t  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%;  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%S  +19 h  .t\PRV.dial :air P%  +18 h  P%c% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %e' +251 h ' .t\PRV.dial :waste2 P%\\ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb % ' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&31 +2 h 1 M&& h & M&H% h %\  .t\Elbow.seek FlushPuck::Garage M&h(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&/ +150 h / M&=& h &- M&"% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &1' +91 h ' .t\CC.seek :closed C'\ (W +416 h (W C'o& h &W .tHand.open M&(1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  C'{& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%: @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%N  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%L  +76 h  .tdelay 2 seconds +200 P%~% h % .t\PS.seek 1.499ml P%@' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%I  +38 h  .t\PS.seek 0ml P%i' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%f +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%y% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %>'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%n% h %r .t\PS.seek 0.475ml P%/' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%b  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Z +75 h .t\PTV.dial :PRV,avoiding: :puck P%V @ +75 h @ .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%-  +75 h  .t\PS.seek 2.5ml P%0' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%v +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%U' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%+  +75 h  .t\PS.seek 2.5ml P%)' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%q +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%<% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %%' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'R & +50 h & .t\AWV.select :waste C' +7 P%$% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %j'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'X { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %G' +1313 h ' .t\PSV.dial :air P%!  +37 h  .t\PS.seek 2.5ml P%p' +2610 h ' .t\PSV.dial :PML P%A +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '^& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *k  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +91 h P%_% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%9' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*d I +129 h I F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P%u' +1597 h ' .t\PSV.dial PSR P%} +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%E' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +22 h ' .t\PSV.dial PSR P%& +21 h  F*K% h %6 ."MFBPCR"\AS.seek 0.329ml F*?'ͯ +92 h P%C% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%'' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%D  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*O% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *z' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +217 ."MFBPCR"\ATV.dial :AIV F*W I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F**' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%l +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%6' +2610 h ' .t\PSV.dial PSR P%# +113 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +38 h  .t\PS.seek 0ml P% ' +739 h ' ."MFBPCR"delay 5 seconds +500 F*Q9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%J +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%,' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%;' +2609 h ' .t\PSV.dial PSR P% +113 h P%S% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%c  +38 h  .t\PS.seek 0ml P%[' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%\\ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%e' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%H  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %5' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*4 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *`'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%=' +2610 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %|' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*g  +43 h  F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *]'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*o% h %Q ."MFBPCR"\AS.seek 0.01ml F*(' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %{' +619 h ' ."MFBPCR"\ATV.dial :waste F*: n +128 h n ."MFBPCR"\AS.seek :empty F*1'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*N  +86 h  F*L% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *~' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*@% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *I'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'f% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'y' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  '  +38 h  +113  .t\CBV.dial :waste1 C'n @ +38 h @ .t\CS.seek 0ml C'>'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*b  +22 h ."MFBPCR"delay 3 seconds +300 F*/% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'  +37 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'Z'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*V  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*-'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*v% h %& ."MFBPCR"\AS.seek 0.092ml F*0'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'+' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'q'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&X/ +150 h / .t\CC.seek :open C'j( +416 h ( M&!& h &ӷ M&G% h % .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &p(- +264 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&1 +150 h 1 M&_% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'd& h & M&M% h %\  A(w% h %X A(x. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (9( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (}& h &  M&u& h &- M&F% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +166 h ( M&m& h & A(K& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(C& h &  ."WCR"\SE.seek 0.71 Pucks below Top Plate A('K +253 h 'K A(E& h &  M&& h & ."WCR"\Elbow.seek 2 M&?( +7 h ( M&D& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&O'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &'/ + h '&0 ."MFBPCR"delay 3 seconds +149 h / ."downElevator"\SE.seek :down A('X M&.& h & M&W% h %8 ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +60 ."MFBPCR"\ATV.dial :waste2 F*z % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +39 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' ."WCR"\CC.seek :closed C'6(W +416 h (W ."WCR"Hand.open M&81 +13 h 'R ."MFBPCR"\ATV.dial :air2 F*a  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *Q' +115 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)*+ +7 h + S)J% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C't  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *V' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F* % h % ."MFBPCR"\AS.seek 0.279ml F*v'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*- n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*U % +43 h % F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*< I +21 h I F*R% h %v ."MFBPCR"\AS.seek 0.059ml F*$'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*X % +21 h % F*P% h %H ."MFBPCR"\AS.seek 0.069ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*!% h % ."MFBPCR"\AS.seek 0.769ml F*G' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*_'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*A I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *d' +4416 h ' ."MFBPCR"\ATV.dial :waste F*M n +129 h n ."MFBPCR"\AS.seek :empty F*w'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*x % +43 h % F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*k% h % ."MFBPCR"\AS.seek 0.579ml F*m' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %  ."MFBPCR"\AS.seek 0.604ml F*p'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*E% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*D n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*''V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *W'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*&% h %V ."MFBPCR"\AS.seek 0.59ml F*z' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*7 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*#% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*? I +86 h I F*0% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*j% h % ."MFBPCR"\AS.seek 0.13ml F*a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*J' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h % ."MFBPCR"\AS.seek 0.255ml F*t'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*9% h %X ."MFBPCR"\AS.seek 0.24ml F**' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*, @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*3'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*\\'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*H  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *4'\  +1032 h '\  F*Y% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*e% h %(A ."MFBPCR"\AS.seek 0.6ml F*='\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*g% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *5' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*" % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0717h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*o  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *1'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*T' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*n % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*b n +21 h n ."MFBPCR"delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*-  +65 h ."MFBPCR"\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*+'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226215 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'R  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'P @ +76 h @ +50 S)$% h % ."WCR"\CBV.dial between CSV and waste2b C'\  +94 h  C'!% h % ."WCR"\CSV.dial between air and CTV C's ` +18 h ` ."WCR"\CS.seek 7.288ml C'%' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'^  ."WCR"\CSV.dial CTV C'G  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ')'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'A  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'_  +37 h  C'M% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'x' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '}' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'd @ +75 h @ +50 S)w% h % ."WCR"\CBV.dial between CSV and waste2b C'F  +94 h  C'k% h % ."WCR"\CSV.dial between air and CTV C'u ` +56 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'C  'm  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'E  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'D  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C''' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)&% h % ."WCR"\CBV.dial between CSV and waste2b C'W  +94 h  C'7% h % ."WCR"\CSV.dial between air and CTV C'# ` +57 h ` ."WCR"\CS.seek 7.288ml C'z' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'?  'O  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'j  +150 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&8% h %\  A(% h %X A(a. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ( (( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (l& h &( M&J& h &ӷ M&r% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &9( +142 h ( M&t& h & A(S& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(*& h &( ."WCR"\SE.seek 18.71 Pucks below Top Plate A(c' +77 h ' M&;& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &'C +26 h 'C ."WCR"\Elbow.hold M&[, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(3- h -+ ."WCR"\SE.seek 19.04 Pucks below Top Plate A(H' +27 h ' M&% h %C A(\\& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&Y'o +20 h 'o M&4& h & A(e& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 20.04 Pucks below Top Plate A( ' +37 h ' M&=& h & M&g% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&"(ӷ +143 h (ӷ M&5& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&o' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C']( +415 h ( A(`& h &( A(h& h &( M&Q& h &ӷ M&:% h % ."WCR"\Forearm.seek :retract M&N'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &(( +143 h ( M& & h & A(@& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &L'C +92 h 'C ."WCR"\Elbow.hold M&~, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &f1 +150 h 1 M&1% h %C A(& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&i'o +20 h 'o M&& h & A(n& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &y' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(/'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (% h %X M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +90 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'>& h & .tHand.open M&21 +2 h 1 M&& h & M&V% h %\  .t\Elbow.seek FlushPuck::Garage M&I(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.seek :garage M&v'η +91 h 'η .tHand.close M&U/ +150 h / M& & h &- M&Z% h %η .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'$(W +416 h (W C'\ & h &W .tHand.open M&!1 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'G& h &W C'^% h % .t\CSV.dial CSR C'%  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C')'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'A% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +463 h '[ .t\CSV.dial :waste C'M +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'x& h &W C'd% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'F'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'u% h %p .t\CS.seek 7ml C'}'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'm  'C  +38 h  +112  .t\CBV.dial :waste1 C'K @ +75 h @ .t\CS.seek 0ml C'E'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'D% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +38 h @ C''% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  +38 h  .t\CBV.dial :waste2b C'W  +75 h  .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing upper manifold C'#& h &W C'.% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'O'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +38 h @ C'% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'j  +38 h +75 .t\CTV.dial :air C'8  +151 h .t\CS.seek 0ml C''4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'l +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C'r& h &W C't% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.seek 3ml C'*'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C';% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'3'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'4'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'e& h &W C'% h %4 .t\CSV.dial CSR C'=  +150 h  C'g% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'9' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'5' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Y'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'H  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'['P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'6  +38 h  +113  .t\CBV.dial :waste1 C'` @ +37 h @ .t\CS.seek 0ml C'h'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'Q  +37 h  C':% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +710 h ' ."connect"\CSV.dial CTV C'o  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'"  +76 h  .t\CS.seek 0ml C'f'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'1  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T 'N  +38 h +75 .t\CTV.dial :air C'(  +151 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'> +38 h .t\CS.seek 0ml C'2'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'V% h %4 .t\CSV.dial :flush C'L  +38 h  .t\CS.seek 5ml C'i'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'/ +113 h .t\CTV.dial :air .\CS.seek 0ml C'y'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'~ +38 h .t\CS.seek 0ml C'U'4 +507 h '4 C' & h &W C'Z% h %4 .t\CSV.dial :flush C'b  +37 h  .t\CS.seek 3ml C'v'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'q% h %0 .t\CS.seek 5ml C'I'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'<'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +506 h '0 .t\CSV.dial :waste C'\ +76 h .t\CS.seek 0ml C'!'4 +506 h '4 #t\Flushing lower manifold's waste1 port C's& h &W C'G% h %4 .t\CSV.dial :flush C'^  +38 h  .t\CS.seek 5ml C'%'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'P% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'A  ')  +37 h  +113  .t\CBV.dial :waste1 C'M @ +38 h @ .t\CS.seek 0ml C'_'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'x& h &W C'd% h %4 .t\CSV.dial :flush C'w  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'u% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'm  +38 h  .t\CBV.dial :waste2b C'C  +75 h  .t\CS.seek 0ml C'K'4 +766 h '4 #tDisinfecting external Intake with kill C'}& h &W C'% h %4 .t\CSV.dial CSR C'D  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'E'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'p' +416 h ' ."connect"\CSV.dial CBV C'' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'&  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'W' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +607 h '4 .t\CSV.dial CSR C'.  +113 h  C'?% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'z' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'O'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C'a( +415 h ( M&r& h &ӷ M&t% h % .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &J(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&e1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &=(- +6 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&c/ +150 h / M&& h &- M&9% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +264 h (ӷ M&H& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'[(W +416 h (W C'& h &W .tHand.open M&61 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Q& h &W C':% h %4 .t\CSV.dial CSR C'h  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'o'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '"% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '@'[ +463 h '[ .t\CSV.dial :waste C'1 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'f& h &W C'% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +75 h .t\CTV.dial :intake C'n  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'>  +76 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'i +113 h .t\CTV.dial :air .\CS.seek 0ml C'/'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +506 h '0 .t\CSV.dial :waste C'y +38 h .t\CS.seek 0ml C'~'4 +507 h '4 C' & h &W C' % h %4 .t\CSV.dial :flush C'Z  +37 h  .t\CS.seek 3ml C'b'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +37 h @ C'% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'v'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +506 h '0 .t\CSV.dial :waste C'< +76 h .t\CS.seek 0ml C'\ '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&]& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +7 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&!' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'P( +416 h ( M&%& h &ӷ M&A% h % .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &d'η +91 h 'η .tHand.open M&w1 +150 h 1 M&_% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST08-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:00:05CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST08-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0717h1500ml.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F*C % +86 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *}'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*W  +64 h  F*.% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F*l % +86 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *X' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +21 h F*r% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*;'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*S  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0719h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*H  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*a n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Q  +21 h ."MFBPCR"delay 2 seconds +200 F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*Y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*"  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*1' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*@  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*('\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +22 h n ."MFBPCR"delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.6ml F*>'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*V' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*L'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0719h1500ml.pcr F*/% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*-  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*U% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +21 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +21 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*^  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *5'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*x  +31 h  ."MFBPCR"\ATV.dial :air3 F*s % +87 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*d% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*m'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *.'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0721h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ARV.dial :carrier F*?  +10 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*l  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*r n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*X% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*S'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*3  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*9  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*J  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0721h1500ml.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*6% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +21 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +21 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*f  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +65 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*>  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*i% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*L'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V @CEST1538949616.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0800h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*]  +11 h  F*5% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*x  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*s  +22 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*) I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*d% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*u n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*C  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*m'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*D'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*} n +21 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0800h1500ml.pcr F*.% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*z  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *l'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*0'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*c  +32 h  ."MFBPCR"\ATV.dial :air3 F*4 % +86 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0802h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*@  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*>  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*V I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*, % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*-  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*L'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*y  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*~'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +22 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*I  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*5 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0802h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*^ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*[  +151 h F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *x' +423 h ' ."MFBPCR"\ATV.dial :ASV F*s n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*w  +43 h  F*!% h % ."MFBPCR"\AS.seek 0.62ml F*)'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*d n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *u'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*+'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*_ % +43 h % F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*m% h % ."MFBPCR"\AS.seek 0.71ml F*}' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*W% h % zG ."MFBPCR"\AS.seek 0.365ml F*&' +7161 h ' ."MFBPCR"\ATV.dial :waste F*? n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*p% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*j% h % ."MFBPCR"\AS.seek 0.579ml F*7' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %  ."MFBPCR"\AS.seek 0.604ml F*l'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*X% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*t  +22 h  ."MFBPCR"\AS.seek 0ml F*$'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*S% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h % q ."MFBPCR"\AS.seek 0.605ml F*c'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +21 h F*9% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F**' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*J'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *='V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *H'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*a n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +202 h 'R ."MFBPCR"\SPE.coast F*:  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*6'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*Q n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y  +43 h  F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*o I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*f  +43 h  F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *@': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*1% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*T n +65 h n F*n% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *(' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*> I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *i': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*,% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b */'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*-% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *y'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *L': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*U n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *Z'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*~ n +151 h n ."MFBPCR"\AS.seek 0ml F*q'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*b % +43 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*<% h % ."MFBPCR"\AS.seek 0.96ml F*]'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*A% h % ."MFBPCR"\AS.seek 0.97ml F*%'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*s I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *!'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*u  +43 h  F*_% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *C' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *+' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*m'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*D  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +43 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*W'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*#'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +64 h  F*z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*t  +32 h  ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*4  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *H'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*` % +108 h % ."MFBPCR"\AS.seek 0ml F*Q'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + C'5 M&6 P%5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990725 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )Y h  C' 'h h  P% %6 h  F* *o h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *O% h %V .t\AS.configure ASconfig S)f% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'N& S)#.0X  A(#QK-0-\ b h  .t\CH.configure CHconfig C'\ 2 .t\PH.coast P%(  h  .t\PH.configure PHconfig P%\ 2 +29 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 #\All dwarf controllers running firmware version 3.99 S) +6 .tValve.reset C' P% F* +6 .t\PCR.off .\PCR.pidtemp= 50,0.1,0,300,25,25 .\PCR.caltemp= 2.7073,0.0373,0 C'% h %p P%% ."rdySampler"Intake.select :close M&V% ."rdySampler"\Exhaust.select :close h %= ."rdySampler"\CTV.dial :air M&2% h %q ."rdyProcessing"\PSV.dial :air h %= C'  P%,  .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &i'\ h ."rdySampler"\CSV.dial :waste1 C' h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)- h  S)/  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M& / .t\Elbow.jog 1500 M& * +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'y'4 S)#.0X 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:01:05CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #8) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&1 +150 h 1 M&s& h &M M&%% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &w(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &d'η +91 h 'η .tHand.close M&)/ +150 h / M&u& h &- M&_% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'F& h &W .tHand.open M&m1 +150 h 1 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C''& h &W C'K% h %4 .t\CSV.dial CSR C'  +150 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'E'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '?'[ +463 h '[ .t\CSV.dial :waste C'W +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '.'4 +2009 h '4 #t\Purging external Intake C'p& h &W C'&% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z '#  h +75 .t\CTV.dial :intake C'R  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'8'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'7  +75 h #t\Flushing upper manifold C'r& h &W C'X% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'$% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'S'4 +507 h '4 C';& h &W C'g% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.seek 3ml C'3'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'% h %0 .t\CS.seek 5ml C'9'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'*'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&J& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C':( +416 h ( M&a& h &ӷ M&`% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +264 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &P'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M&{% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:01:11CEST30-Sep-18\\n{1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:01:05CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*k% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'^& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&Y% h %\  A(h% h %X A(6. h ., A(o& h &  .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&O& h &- M&f% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &N( +6 F*"% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *1' +160 h ( M&@& h & A(T& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &n' +89 h ' A(& h &  .t\SE.seek -0.29 Pucks below Top Plate A(>'\\ +265 h '\\ A((& h &  M&& h & .t\Elbow.seek 2 M&V( +6 h ( M&& h & A(2& h &  .t\Forearm.seek 2 + 300 counts M&'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&,& A(-'X h & M&/% h %8 .t\Forearm.seek :retract M& '\ +90 h '\  .t\Elbow.seek CC M&~(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&51 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)b+ +6 h + S)q% h % C'A& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +151 h  .t\CSV.dial CSR C'  +75 h  C'V% h %4 #t\Pulling 1ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1ml C'#P d@b ' ' +350 h ' .tdelay 5 seconds +500 .\CSR.select :air C' +6 '-% h % .t\CS.seek 6ml C':' +1372 h ' .tdelay 2 seconds +200 .\CSV.dial :waste1 C'5 +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b 'i' +710 h ' .t\CSV.dial CSR C'q  +150 h  C'A% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'v' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %  .t\CS.seek 4.5ml C'U'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'b  +38 h  .t\CS.seek 1.275ml C's' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'%   '9 h 9d +100 C'!9 h 9d +100 C'x9 h 91d +100 C'9 h 9J d +100 C'19 h 9bd +100 C'u9 h 9zd +100 C'_9 h 9d +100 C'<9 h 9d +100 C'I9 h 9 d +100 C'9 h 9$d +100 C'+9 h 9(d +100 C'w9 h 9 ,d +100 C'9 h 9$0d +100 C'[9 h 9<4d +100 C'd9 h 9S8d +100 C'D9 h 9kd +100 C'X9 h 9Td +100 C'R9 h 9kd +100 C'l9 h 9d +100 C'89 h 9d +100 C'$9 h 9d +100 C't9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'.9 h 9 d +100 C'r9 h 96d +100 C'9 h 9Ld +100 C';9 h 9bd +100 C'g9 h 9xd +100 C'09 h 9d +100 C'S9 h 9d +100 C'49 h 9d +100 C'9 h 9d +100 C'e9 h 9d +100 C'39 h 9d +100 C'c9 h 9d +100 C'9 h 9(d +100 C'J9 h 9>d +100 C'99 h 9Td +100 C'=9 h 9id +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9d +100 C'9 h 9d +100 C'`9 h 9  d +100 C'9 h 9 d +100 C'C9 h 9 *d +100 C'B9 h 9 ?d +100 C'|9 h 9 Td +100 C'\ 9 h 9 j d +100 C'a9 h 9 $d +100 C'\\9 h 9 (d +100 C'H9 h 9 ,d +100 C'k9 h 9 0d +100 C'Y9 h 9 4d +100 C'h9 h 9 8d +100 C'69 h 9 9 h 9 d +100 C'9 h 9 1d +100 C'V9 h 9 Dd +100 C'-9 h 9 Xd +100 C' 9 h 9 kd +100 C'59 h 9 ~d +100 C':9 h 9 d +100 C'q9 h 9 d +100 C'A9 h 9 d .t\CH.hold 5:00,85C C'i   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'v'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'b @ +113 h @ .t\CSV.dial :air C'U @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'!  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'x @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'1 @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'u'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'_ +112 h .t\CBV.dial CSV C'<  +19 h C'I% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'+  +38 h C'w% h %p .t\CS.seek 7ml C''i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'[ @ +37 h @ .t\CS.seek 10ml C'd' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'D ` +132 h ` #tLysing Complete C''& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&K% h %\  A(% h %X A(F. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (m(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&)& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &E( +143 h ( M&W& h & A(?& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &p' +89 h ' A(&& h &( .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' M& & h & A(#& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &z'C +25 h 'C .t\Elbow.hold M&7, +7 h , .t\Elbow.reconfigure TightElbow A(j- h -+ M&$&.,(\ #b .t\SE.seek 20.04 Pucks below Top Plate A(*' +27 h ' M&X% h %C A(R& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&l'o +21 h 'o M&8& h & A($& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &t' +22 h ' .t\SE.seek 21.04 Pucks below Top Plate A(' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +90 h '\  .t\Elbow.seek CC M&r(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'S( +416 h ( A(4& h &( A(& h &( M&e& h &ӷ M&3% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&J& h & A(9& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &='C +93 h 'C .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&}'o +21 h 'o M&& h & A(`& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(C% h % ."downElevator"\SE.seek :down A(B'X +53 h 'X ."downElevator"\SE.coast A(5 +47 (|% h %X M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +89 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'\\ @ +19 h @ .t\CS.seek 10ml C'H' +7 h ' .t\CSV.dial between air and CTV C'k ` +18 h ` .t\AIV.dial :bypass C'Y { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'h& h & .tHand.open M&61 +2 h 1 M&o& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&{(- +166 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&^/ +150 h / M&T& h &- M&P% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +265 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'2(W +416 h (W C'Q& h &W .tHand.open M& 1 +150 h 1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%,% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%N' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%n  +37 h  .t\PS.seek 0ml P%/' +1597 h ' .t\PSV.dial PSR P%G +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%>  C'  h +75 .t\PSV.dial :mixloop P%V +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%-  h .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%5  +38 h  .t\PS.seek 2.5ml P%:' +2609 h ' .t\PSV.dial :mixloop P%q +38 h .t\PS.seek 0ml P%A' +2610 h ' .t\PSV.dial :air P%i  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +38 h .t\PS.seek 0ml P%b' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&1/ +150 h / .t\CC.seek :open C'( +416 h ( M&_& h &ӷ M&<% h % .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&[1 +150 h 1 M&%% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'D& h & M&'% h %\  A(K% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (F( +357 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&)& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &W( +166 h ( M&?& h & A(&& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(#& h & .t\SE.seek 20.71 Pucks below Top Plate A(~' +56 h ' A(j& h & M&X& h & .t\Elbow.seek 3 M&R( +7 h ( M&8& h & A($& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&p& A(L'X h & M&E% h %B .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&z(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'*(W +416 h (W .tHand.open M&t1 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +90 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'.  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'4'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'e'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%3  +38 h  .t\PS.seek 2.5ml P%J' +2610 h ' .t\PSV.dial :mixloop P%9 +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%g  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'` @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'C  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'c ` +18 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'|' +2647 h ' .t\CSV.dial CTV C'\  +38 h  .t\CS.seek 0ml C'\\'4 +2647 h '4 .t\CBV.dial :waste1 C'k @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'Y  +150 h C'h& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&6% h %\  A(o% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (=( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (}& M&& h &  &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(B'Z .t\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&m' +91 h ' .tHand.close M&d/ +150 h / .t\CC.seek :open C'( +23 h 'Z +393 ( A(^& h &  A(T& h &  M&P& h &ӷ M&a% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &M( +143 h ( M&(& h & A({& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +92 h '  .t\Elbow.hold M&S, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &Q1 +150 h 1 M& % h %  A(O& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&,& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A(N% h %Z ."downElevator"\SE.seek :down A(G'X +100 (/% h % M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%V  + A(6 +112 h  P%-% h % .t\PS.seek 0.499ml P% ' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%5 +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%q  +37 h .t\CBV.dial AIV C':  +38 h .t\PRV.dial between CBV and air P%i  +19 h  .t\PRV.dial :air P%A  +19 h  P%s% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%U @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %!' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'x& h & .tHand.open M&11 +2 h 1 M&@& h & M&Z% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&+/ +150 h / M&w& h &- M&v% h %η .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +264 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'K(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%? @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%&  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%#  +75 h  .tdelay 2 seconds +200 P%u% h % .t\PS.seek 1.499ml P%j' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%X' +1094 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.seek 0ml P%$' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%p% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %E'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%W  +76 h .tdelay 2 seconds +200 P% % h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%;  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%~  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %R' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%7  +76 h  .t\PS.seek 2.5ml P%.' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%l' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%F  +76 h  .t\PS.seek 2.5ml P%H' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%4 +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%3% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %b' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'9 & +50 h & .t\AWV.select :waste C' +7 P%g% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %r'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %J' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%` +38 h .t\PS.seek 0ml P%C' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'c& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*e @@T *\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%| +21 h  ."MFBPCR"delay 20 seconds +92 h P%k% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*h I +129 h I F*6% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *o'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%}' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%f' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%" +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%d' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*z  +22 h ' .t\PSV.dial PSR P%0 +21 h  F**% h %6 ."MFBPCR"\AS.seek 0.329ml F*^'ͯ +92 h P%T% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%P' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*a % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%{  +37 h  .t\PS.seek 0ml P%m' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b * ' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%O +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%,' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*N% h % ."MFBPCR"\AS.seek 0.469ml F*' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%M +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P%> +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%B  +38 h  .t\PS.seek 0ml P%V' +739 h ' ."MFBPCR"delay 5 seconds +500 F*-9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*n n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*G% h % ."MFBPCR"\AS.seek 0.041ml F*='% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%5' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial PSR P%: +113 h P%i% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%s  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%1 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %@'k +2678 h 'k .t\PSV.dial :waste1 P%Z  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*< I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *S'V +850 h ' .t\PSV.dial :air P%+  +113 h  .t\PS.seek 2.5ml P%w' +2609 h ' .t\PSV.dial :mixloop P%v +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %I' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*D  +43 h  F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *['Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +619 h ' ."MFBPCR"\ATV.dial :waste F*? n +129 h n ."MFBPCR"\AS.seek :empty F*''R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*&  +86 h  F*#% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *u' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*!  +42 h F*j% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *8'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'X  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'p' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  'W  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'E'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*;  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*~'ݏ +110 h '4 .t\CSV.dial CSR C'L  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*R% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*7'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*t n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*.'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'F' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'4'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&3& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M&b' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'r( +416 h ( M&& h &ӷ M&J% h % .t\Forearm.seek :retract M&`'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\ 'η +91 h 'η .tHand.open M&|1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'h& h & M&6% h %\  A( % h %X A(y. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Y( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&}& h &- M&"% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &f( +166 h ( M&z& h & A(*& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &0' +90 h ' A(T& h & ."WCR"\SE.seek 19.71 Pucks below Top Plate A(o' +66 h ' A(d& h & M&a& h & ."WCR"\Elbow.seek 3 M&^( +6 h ( M&{& h & A(& h & ."WCR"\Forearm.seek 3 + 300 counts M&Q'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &P/ +150 h / ."downElevator"\SE.seek :down M&(& A(O'X h & M&% h %B ."WCR"\Forearm.seek :retract M&N'\ +39 h '&0 ."MFBPCR"delay 3 seconds +27 h 'X ."downElevator"\SE.coast A(5 +25 h '\  ."WCR"\Elbow.seek CC M&m(ӷ +143 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' ."WCR"\CC.seek :closed C'>(W +14 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +359 h (W ."WCR"Hand.open M&B1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +50 h 'R ."MFBPCR"\ATV.dial :air2 F*n  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *G' +19 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)% h % C'V& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +38 h  +113  .t\CBV.dial :waste1 C'S @ +37 h @ .t\CS.seek 0ml C'2'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'G  +38 h  C'?% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'v  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C''  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'X'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ '&  +37 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'L'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C'E +38 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'R& h &W C'% h %4 .t\CSV.dial :flush C'#  +37 h  .t\CS.seek 5ml C'$'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'p% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'u +37 h .t\CS.seek 0ml C'l'4 +507 h '4 C't& h &W C'% h %4 .t\CSV.dial :flush C';  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'8'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'3'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'['0 +506 h '0 .t\CSV.dial :waste C'K +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C')& h &W C'c% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.seek 5ml C'b'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'%% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV C'`  .t\CTV.dial CSV C'H  +37 h  +113  .t\CBV.dial :waste1 C'6 @ +38 h @ .t\CS.seek 0ml C'k'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'y& h &W C'h% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +37 h @ C'}% h %p .t\CS.seek 7ml C'"'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'z  +38 h  .t\CBV.dial :waste2b C'T  +75 h  .t\CS.seek 0ml C'*'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'd% h %4 .t\CSV.dial CSR C'  +38 h  C'C% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'a'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'P .t\CBV.dial CSV C'{  +38 h +75 .t\CTV.dial :intake C'0  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'f  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Q'4 +607 h '4 .t\CSV.dial CSR C'(  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'M'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C' ' +1372 h ' .t\CSV.dial CBV C'o +113 h .t\CS.seek 0ml C'O'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&.& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'B( +416 h ( M&/& h &ӷ M&,% h % .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&q1 +150 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&<1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &w(- +6 h (- M&D& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&s/ +150 h / M&=& h &- M&Y% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &x' +91 h ' .t\CC.seek :closed C' (W +415 h (W C'& h &W .tHand.open M&>1 +150 h 1 M&S% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'G& h &W C'?% h %4 .t\CSV.dial CSR C'2  +113 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'j'[ +463 h '[ .t\CSV.dial :waste C'' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'& h &W C'X% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'L'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'E  +75 h #t\Flushing upper manifold C'W& h &W C'R% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'#'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'p'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'$ +113 h .t\CTV.dial :air .\CS.seek 0ml C'7'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'~'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'u'4 +507 h '4 C'V& h &W C't% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C';'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'% h %0 .t\CS.seek 5ml C'4'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +506 h '0 .t\CSV.dial :waste C'3 +76 h .t\CS.seek 0ml C'K'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +7 h (ӷ M&c& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'%( +416 h ( M&b& h &ӷ M&`% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &6(- +264 h (- M&r& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &h'η +91 h 'η .tHand.open M& 1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST09-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:115\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:02:52CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST09-Oct-18" +263628 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0817h1500ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*z  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*0% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*f  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*-'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*q% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *@'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0819h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*Z  +11 h  F*+% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*B n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*G  +22 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *2'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*!  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*v  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*j  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*W  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*#'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0819h1500ml.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*~  +21 h ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*['q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*|% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *_'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +32 h  ."MFBPCR"\ATV.dial :air3 F*) % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*e'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +21 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0821h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*Q% h %V ."MFBPCR"\AS.seek 0.5ml F*M'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *.'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*9  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*/ n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*n' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*m I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.53ml F*N' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*q % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*-% h % ."MFBPCR"\AS.seek 0.536ml F*i'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*1% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Z'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*@ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0821h1500ml.pcr F*w% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *G'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*!'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*O% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +65 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +31 h  ."MFBPCR"\ATV.dial :air3 F*L % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*p'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +21 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +21 h F*V% h %m ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V @CEST1539036009.69 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0900h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ARV.dial :carrier F*[  +10 h  F*_% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *`'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*)  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*H I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*k' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*} n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*e' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*T  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*C  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*{  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct0900h1500ml.pcr F*(% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*o % +86 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*^'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +64 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +21 h F*=% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *?'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0902h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*&  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*X'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\\ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *R'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*5 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*p' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*~  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.536ml F*#'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*l n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*|'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct0902h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*J @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *y' +424 h ' ."MFBPCR"\ATV.dial :ASV F*) n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*H'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*h n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *}'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*F'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*k % +43 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *"' +424 h ' ."MFBPCR"\ATV.dial :air3 F*e% h % ."MFBPCR"\AS.seek 0.53ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*z% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*C' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +21 h F*a% h % Ű ."MFBPCR"\AS.seek 0.72ml F*{' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*f n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*0' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*( % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*.% h % ."MFBPCR"\AS.seek 0.579ml F*M' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*^' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*m n +86 h n ."MFBPCR"\ASV.dial :wasteR F*,  +22 h  ."MFBPCR"\AS.seek 0ml F*g'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *-'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*N % +108 h % F*n% h %V ."MFBPCR"\AS.seek 0.01ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*i% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +21 h % ."MFBPCR"delay 3 seconds +300 F*<% h % q ."MFBPCR"\AS.seek 0.605ml F*s'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +21 h F*Y% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*=  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *w'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*+  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *Z'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*B n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +202 h 'R ."MFBPCR"\SPE.coast F*?  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*>'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*G n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*x  +43 h  F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *!' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *j': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*'% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *X'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*\\ n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*E I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*W  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *$': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*R  +64 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *7'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*~% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *V' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*p I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*t  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *#': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*l n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*u n +150 h n ."MFBPCR"\AS.seek 0ml F*4'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*; % +43 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*3% h % ."MFBPCR"\AS.seek 0.96ml F*['0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*|' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*`% h % ."MFBPCR"\AS.seek 0.97ml F*b'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*) I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *r'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*}  +43 h  F*k% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *F' +9988 h ' ."MFBPCR"\ATV.dial AIV F*e I +43 h I ."MFBPCR"\AS.seek 0.02ml F*"' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*z'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*d% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *a'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*0'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*o  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*.  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*n% h % ."MFBPCR"\AS.seek 0.81ml F*-'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*? n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *Z'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*S % +108 h % ."MFBPCR"\AS.seek 0ml F*G'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2991358 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )x h  C' '2 h  P% %> h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *!  h  .t\SPE.configure SPEconfig F*\ 2 *O% h %V .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'&& S)#.0X 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:03:51CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #9) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'`& h & .tHand.open M&|1 +150 h 1 M&)& h &M M&b% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &h'η +91 h 'η .tHand.close M&H/ +150 h / M&}& h &- M&k% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +264 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'd(W +416 h (W C'"& h &W .tHand.open M&z1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'P& h &W C'*% h %4 .t\CSV.dial CSR C'C  +150 h  C'f% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'a'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '{% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '('4 +2009 h '4 #t\Purging external Intake C'& h &W C'0% h %4 .t\CSV.dial :flush C'o  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q '  h +75 .t\CTV.dial :intake C'9  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'M  +75 h #t\Flushing upper manifold C'/& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C','p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +37 h @ C'g% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'N +113 h .t\CTV.dial :air .\CS.seek 0ml C'n'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'1 +37 h .t\CS.seek 0ml C'i'4 +507 h '4 C'-& h &W C'D% h %4 .t\CSV.dial :flush C'<  +38 h  .t\CS.seek 3ml C'A'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'% h %0 .t\CS.seek 5ml C'Y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C's'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +507 h '0 .t\CSV.dial :waste C'= +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +7 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'?( +416 h ( M&B& h &ӷ M&S% h % .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &%(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&]1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:03:57CEST30-Sep-18\\n{1 => 22, 2 => 2, 3 => 20, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:03:51CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*I% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'J& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&x% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(2% h %X A(>. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*!% h %&K ."readyMFB"\ATV.dial :waste2 F*O % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (&& h & M&v& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &'( +166 h ( M&j& h & A(\\& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(X& h & .t\SE.seek 18.71 Pucks below Top Plate A(E' +77 h ' A(& h & M&L& h & .t\Elbow.seek 3 M&W( +6 h ( M&& h & A(R& h & .t\Forearm.seek 3 + 300 counts M&5'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(~'X M&& h & M&7% h %B .t\Forearm.seek :retract M&V'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&u(ӷ +143 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'_(W +416 h (W .tHand.open M&1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S);+ +6 h + S)4% h % C'`& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +19 h  C'!% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'O  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '&'4 +2609 h '4 C'v% h %4 S)% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'G @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'9 h 9Td +100 C'99 h 9kd +100 C' 9 h 9d +100 C'9 h 9d +100 C'g9 h 9d +100 C',9 h 9d +100 C'N9 h 9d +100 C'm9 h 9d +100 C'n9 h 9 d +100 C'(9 h 9 d +100 C'/9 h 96d +100 C'q9 h 9Ld +100 C'-9 h 9bd +100 C'D9 h 9xd +100 C'^9 h 9d +100 C'i9 h 9d +100 C'U9 h 9d +100 C'9 h 9d +100 C'<9 h 9d +100 C'A9 h 9d +100 C's9 h 9d +100 C'=9 h 9(d +100 C'9 h 9>d +100 C'Y9 h 9Td +100 C'w9 h 9id +100 C'19 h 9d +100 C'+9 h 9d +100 C':9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'd9 h 9d +100 C'S9 h 9  d +100 C'69 h 9 d +100 C'T9 h 9 *d +100 C'9 h 9 ?d +100 C'\ 9 h 9 Td +100 C']9 h 9 j d +100 C'B9 h 9 $d +100 C'@9 h 9 (d +100 C'Z9 h 9 ,d +100 C'I9 h 9 0d +100 C'x9 h 9 4d +100 C'29 h 9 8d +100 C'>9 h 9 1 +2 h 1 M&!& h & M&O% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&G'η +91 h 'η .tHand.close M&J/ +150 h / M&j& h &- M&\\% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'K(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%R +151 h P%7% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%'' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%c +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%t' +2610 h ' ."connect"\PRV.dial CBV P%E  .t\CBV.dial PRV C'5  h +75 .t\PSV.dial :mixloop P%~ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%V  h .t\PS.seek 0ml P%W' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%?' +2609 h ' .t\PSV.dial :mixloop P%4 +38 h .t\PS.seek 0ml P%`' +2610 h ' .t\PSV.dial :air P%$  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%) +38 h .t\PS.seek 0ml P%;' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&l& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&|' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'p( +416 h ( M&k& h &ӷ M&3% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &}(- +264 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M& 1 +150 h 1 M&b% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&P% h %\  A(*% h %X A(C. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ("( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&H& h &- M&{% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&& h & A(0& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &u' +90 h ' A(#& h & .t\SE.seek 17.71 Pucks below Top Plate A(f'* +86 h '* A( & h & M&.& h & .t\Elbow.seek 3 M&( +6 h ( M&9& h & A(& h & .t\Forearm.seek 3 + 300 counts M&g'B +23 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &m/ +150 h / ."downElevator"\SE.seek :down A('X M&n& h & M&a% h %B .t\Forearm.seek :retract M&o'\ +86 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .t\Elbow.seek CC M&Q(ӷ +143 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&,1 +150 h 1 M&M% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'(  +150 h  .t\CSV.dial CTV C'_  +19 h  .t\CS.seek 5ml C'U'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'<'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%A  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%Y +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%D  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%/ +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%+  +151 h  .t\PS.seek :empty P%:' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'd  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'S @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'T  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C's ` +19 h ` .t\CSV.dial :air C'= @ +37 h @ .t\CS.seek 10ml C'\ ' +2648 h ' .t\CSV.dial CTV C']  +37 h  .t\CS.seek 0ml C'w'4 +2647 h '4 .t\CBV.dial :waste1 C'@ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'I& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&x% h %\  A(2% h %X A(>. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (!( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (O& h &  M&N& h &ӷ M&6% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &v( +143 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &z' +89 h ' A(h& h &  .t\SE.seek 1.71 Pucks below Top Plate A(1': +244 h ': M&J& h & A(j& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &\\' +25 h '  .t\Elbow.hold M&B, +7 h , .t\Elbow.reconfigure TightElbow A(G- h -+: M&$&.,(\ #b .t\SE.seek 2.04 Pucks below Top Plate A(%' +27 h ' M&L% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&X'8 +21 h '8 M&i& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(&' +36 h ' M&R& h & M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&E/ +150 h / .t\CC.seek :open C'5( +416 h ( A(~& h &  A(V& h &  M&t& h &ӷ M&% h % .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &4( +143 h ( M&?& h & A($& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &`' +92 h '  .t\Elbow.hold M&), +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&l% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&y'8 +21 h '8 M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(K% h % ."downElevator"\SE.seek :down A(k'X +100 (3% h %1 M&|% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +89 h '\  +43 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P% ' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%b +38 h .t\PRV.dial :air P%8  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'P  +38 h .t\PRV.dial between CBV and air P%*  +19 h  .t\PRV.dial :air P%C  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %H' +251 h ' .t\PRV.dial :waste2 P%{ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %e' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&01 +2 h 1 M&#& h & M&}% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&r/ +150 h / M&m& h &- M&n% h %η .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &-' +91 h ' .t\CC.seek :closed C'f(W +416 h (W C'g& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  C'p& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%, @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%M  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%(  +76 h  .tdelay 2 seconds +200 P%_% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%"' +1094 h ' .t\PSV.dial :air P%Z  +38 h  .t\PS.seek 0ml P%U' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%A  +38 h  .tdelay 2 seconds +200 P%;% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Y'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%D  +76 h .tdelay 2 seconds +200 P%/% h %r .t\PS.seek 0.475ml P%+' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%d  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%S  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %T' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%s +75 h .t\PTV.dial :PRV,avoiding: :puck P%= @ +75 h @ .t\PS.seek 0ml P%<' +2610 h ' .t\PSV.dial :air P%]  +75 h  .t\PS.seek 2.5ml P%\ ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%@ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%I  +75 h  .t\PS.seek 2.5ml P%x' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%2 +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%>  +75 h P%O% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %N' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'6 & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'h { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %Q' +1313 h ' .t\PSV.dial :air P%^  +37 h  .t\PS.seek 2.5ml P%J' +2610 h ' .t\PSV.dial :PML P%j +38 h .t\PS.seek 0ml P%G' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'L& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& F*i @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass F*  .t\PSV.dial PSR P%R +21 h  ."MFBPCR"delay 20 seconds +92 h P%7% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%z' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*v I +129 h I F*1% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *\\'6 +66 .t\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%c' +1597 h ' .t\PSV.dial PSR P%X +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%B' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*E  +22 h ' .t\PSV.dial PSR P% +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*~'ͯ +92 h P%V% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%t' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *l' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*K% h % ."MFBPCR"\AS.seek 0.469ml F*W' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%3' +2610 h ' .t\PSV.dial PSR P%| +113 h P%`% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P% ' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*k% h % ."MFBPCR"\AS.seek 0.041ml F*!'% +336 h ' .t\PSV.dial PSR P%: +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%b' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%8 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%P +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%C' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%{ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%H' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %#'k +2679 h 'k .t\PSV.dial :waste1 P%}  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %.' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*9 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *)'V +849 h ' .t\PSV.dial :air P%r  +113 h  .t\PS.seek 2.5ml P%m' +2610 h ' .t\PSV.dial :mixloop P%n +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %F' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %u' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*q  +43 h  F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *a'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*5 I +43 h I F*g% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %p' +619 h ' ."MFBPCR"\ATV.dial :waste F*, n +129 h n ."MFBPCR"\AS.seek :empty F*-'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*M  +86 h  F*(% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *_' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*e  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *Z'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'"  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'A'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C';' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  'D  +38 h  +113  .t\CBV.dial :waste1 C'/ @ +38 h @ .t\CS.seek 0ml C'Y'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*+% h %_ ."MFBPCR"\AS.seek 0.434ml F*d'ݏ +110 h '4 .t\CSV.dial CSR C'S  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C's'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*=  +22 h F*T% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*]'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*< n +108 h n F*@% h %& ."MFBPCR"\AS.seek 0.092ml F*\ '&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'I' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'2'4 +2647 h '4 C'>& h &W #tGaraging FlushPuck in Collection Clamp M&O& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +7 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&N' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'( +416 h ( M&^& h &ӷ M&Q% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &J(- +264 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&R1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'v& h & M&1% h %\  A('% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (z( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (X& h & M&c& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &%( +166 h ( M&E& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(V& h & ."WCR"\SE.seek 16.71 Pucks below Top Plate A(\\'; +95 h '; A(B& h & M&& h & ."WCR"\Elbow.seek 3 M&~( +7 h ( M&$& h & A( & h & ."WCR"\Forearm.seek 3 + 300 counts M&'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down A(?'X M&& h & M&% h %B ."WCR"\Forearm.seek :retract M&K'\ +9 h '&0 ."MFBPCR"delay 3 seconds +82 h '\  ."WCR"\Elbow.seek CC M&(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +137 h (ӷ M&l& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +76 ."MFBPCR"\ATV.dial :waste2 F*| % +15 h ' ."WCR"\CC.seek :closed C'`(W +28 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +388 h (W ."WCR"Hand.open M&&1 +150 h 1 M&y% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +21 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *k' +48 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)W+ +7 h + S):% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'8  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*O I +21 h I F*6% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*h % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*^% h % ."MFBPCR"\AS.seek 0.769ml F*Q' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*R% h % k ."MFBPCR"\AS.seek 0.779ml F*7'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*j I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *v' +4416 h ' ."MFBPCR"\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.seek :empty F*''R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*E' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %  ."MFBPCR"\AS.seek 0.604ml F*J'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*$' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*G  +43 h  ."MFBPCR"\AS.seek 0ml F*t'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*% % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*l' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*4 n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*\\' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*~ I +86 h I F*?% h % ."MFBPCR"\AS.seek 0.12ml F*K' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*\ % h % ."MFBPCR"\AS.seek 0.13ml F*&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*:' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*z% h %X ."MFBPCR"\AS.seek 0.24ml F*W' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*P  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *C'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*b @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*! I +86 h I ."MFBPCR"\AS.seek 0ml F*0'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*{'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*}  +43 h  #"MFBPCR"\Swish #1 of 2 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *9'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*r'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*H% h %(A ."MFBPCR"\AS.seek 0.6ml F*m'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*F'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*q% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *.' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*n % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +3472 h 'V ."MFBPCR"\SPE.coast F*5  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0917h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*g  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*a% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *M'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*e  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *-'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*_  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*A  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*/ % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*D'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*+  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.6ml F*Z'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*d  +43 h  ."MFBPCR"\ASV.dial :waste1 F*]  +65 h ."MFBPCR"\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*I'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*O % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226057 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'6  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'> @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'f  +94 h  C'^% h % ."WCR"\CSV.dial between air and CTV C'o ` +19 h ` ."WCR"\CS.seek 7.288ml C'N' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Q  'L  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'x'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'R  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'j  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'7  +38 h  C'1% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'v @ +75 h @ +50 S)'% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'c ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'E  'V  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'B'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'J' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'G  +38 h  C'$% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C't' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'% @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C'4% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'l' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'|  '~  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '?'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'\\  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'\  +151 h C'K& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&y% h %\  A(% h %X A(&. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ( ( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (8& h &  M&:& h &ӷ M&3% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +142 h ( M&& h & A(*& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &P' +90 h ' A(W& h &  ."WCR"\SE.seek 2.71 Pucks below Top Plate A(') +234 h ') M&& h & A(C& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &!' +26 h '  ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow A(0- M&$&.,(\ #b h -+) ."WCR"\SE.seek 3.04 Pucks below Top Plate A(}' +27 h ' M&#% h %  A({& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&9& h & A(H& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &r' +23 h ' ."WCR"\SE.seek 4.04 Pucks below Top Plate A(' +36 h ' M&m& h & M&q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +89 h '\  ."WCR"\Elbow.seek CC M&n(ӷ +143 h (ӷ M&.& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ ."WCR"\Forearm.seek CC M&g' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'a( +416 h ( A()& h &  A( & h &  M&k& h &ӷ M&,% h % ."WCR"\Forearm.seek :retract M&M'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&e& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &(' +92 h '  ."WCR"\Elbow.hold M&_, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&-% h %  A(A& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&U'8 +20 h '8 M&"& h & A(/& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &;' +22 h ' A(w% h % ."downElevator"\SE.seek :down A(+'X +100 (S% h %  M&D% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&1 +2 A(6 h 1 M&T& h & M&=% h %\  .t\Elbow.seek FlushPuck::Garage M&Z(- +166 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.seek :garage M&@'η +91 h 'η .tHand.close M&/ +150 h / M&<& h &- M&s% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +264 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'f& h &W .tHand.open M&^1 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'L& h &W C'Q% h % .t\CSV.dial CSR C'N  +38 h  C'R% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'x'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '7'[ +462 h '[ .t\CSV.dial :waste C'1 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'v% h %4 .t\CSV.dial :flush C''  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'c% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'V  'E  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'B& h &W C' % h %4 .t\CSV.dial :flush C'G  +75 h  .t\CS.seek 5ml C'$'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'J @ +37 h @ C't% h %p .t\CS.seek 7ml C'%'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'4'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'~  +75 h  .t\CS.seek 5ml C'|'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +37 h @ C'\\% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ 'K  +38 h +75 .t\CTV.dial :air C'y  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +506 h '0 .t\CSV.dial :waste C'8 +38 h .t\CS.seek 0ml C':'4 +507 h '4 C'3& h &W C'% h %4 .t\CSV.dial :flush C'*  +37 h  .t\CS.seek 3ml C'W'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'C'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +506 h '0 .t\CSV.dial :waste C'# +76 h .t\CS.seek 0ml C'{'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'9& h &W C'H% h %4 .t\CSV.dial CSR C'm  +151 h  C'q% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'P'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'z' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'.' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'5  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'}  +113 h  C'r% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'F  '`  +38 h  +113  .t\CBV.dial :waste1 C') @ +37 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'k  +38 h  C',% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV C'g  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'n  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'-  +37 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '"'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w 'M  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'S'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C'Y +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'T& h &W C'=% h %4 .t\CSV.dial :flush C'(  +37 h  .t\CS.seek 5ml C'U'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'd% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +113 h .t\CTV.dial :air .\CS.seek 0ml C'+'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'_ +37 h .t\CS.seek 0ml C''4 +507 h '4 C'<& h &W C's% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'@'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'> @ +38 h @ C'2% h %0 .t\CS.seek 5ml C'Z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'O'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'f +75 h .t\CS.seek 0ml C'^'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'o& h &W C'L% h %4 .t\CSV.dial :flush C'Q  +38 h  .t\CS.seek 5ml C'N'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'x  'j  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C'7'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'v% h %4 .t\CSV.dial :flush C''  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'c% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'V  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'X& h &W C'B% h %4 .t\CSV.dial CSR C'  +38 h  C'G% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'J' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C't '$  +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'%  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'~% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'4'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +1372 h ' .t\CSV.dial CBV C'\\ +113 h .t\CS.seek 0ml C'|'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\ (ӷ +7 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C'&( +416 h ( M&3& h &ӷ M&% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &W'η +91 h 'η .tHand.open M&1 +150 h 1 M&C% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'0& h & .tHand.open M&91 +2 h 1 M&H& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &m(- +7 h (- M&q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&P'η +90 h 'η .tHand.close M&/ +150 h / M&!& h &- M&z% h %η .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +265 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'F& h &W .tHand.open M&`1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'k& h &W C',% h %4 .t\CSV.dial CSR C'  +113 h  C'e% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'g'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'n% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'- +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'A'4 +2009 h '4 #t\Purging external Intake C'& h &W C'"% h %4 .t\CSV.dial :flush C'M  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'/  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'S'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Y  +75 h #t\Flushing upper manifold C'D& h &W C'T% h %4 .t\CSV.dial :flush C'=  +75 h  .t\CS.seek 5ml C'('p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U +113 h .t\CTV.dial :air .\CS.seek 0ml C';'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'_'4 +507 h '4 C' & h &W C'<% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'>% h %0 .t\CS.seek 5ml C'2'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'@'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Z'0 +507 h '0 .t\CSV.dial :waste C'O +75 h .t\CS.seek 0ml C'f'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&a& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&R/ +150 h / .t\CC.seek :open C'( +416 h ( M&N& h &ӷ M&x% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &v'η +91 h 'η .tHand.open M&'1 +150 h 1 M&7% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST10-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:120\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:05:40CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST10-Oct-18" +263658 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0917h1500ml.pcr F*c% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *X'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +32 h  ."MFBPCR"\ATV.dial :air3 F*8 % +87 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*C'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F**  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0919h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ARV.dial :carrier F*}  +11 h  F*r% h %V ."MFBPCR"\AS.seek 0.5ml F*m'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *F'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*)  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*k  +22 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*e  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*n  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.53ml F*-' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*"'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +108 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*/ n +22 h n ."MFBPCR"delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*('{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct0919h1500ml.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*]  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *s'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*>'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*a'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +65 h  F*R% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *.'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*E'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0921h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*4'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*8  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*3 n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*\ ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F**'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*C  +108 h  ."MFBPCR"delay 7 seconds +700 F*0% h %< ."MFBPCR"\AS.seek 0.594ml F*9'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*W  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*!' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*:  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*P% h % \\ ."MFBPCR"\AS.seek 0.326ml F*}'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct0921h1500ml.pcr F*m% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*5  +22 h ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*n'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +21 h F*|% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +65 h  F*M% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Y  +31 h  ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*('m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*+  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V @CEST1539122418.96 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1000h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*R  +42 h  ."MFBPCR"\ARV.dial :carrier F*a  +11 h  F*.% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +22 h ."MFBPCR"delay 2 seconds +200 F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*j I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*v% h %Fj ."MFBPCR"\AS.seek 0.524ml F*7' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*c n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*i' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*V  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*E'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*B  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*X n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*G  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*%  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1000h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*l  +22 h ."MFBPCR"\ATV.dial :air3 F*\\ % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*\ % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *C'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*{ % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*r'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*}'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *-'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1002h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*w n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*= I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*b % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*d' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*]  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*+  +22 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*_'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*O' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*L% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1002h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*Q @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :ASV F*o n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*'  +43 h  F*^% h % ."MFBPCR"\AS.seek 0.62ml F*j'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*v n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *c'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*I'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*7 % +43 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*B' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*E% h % ."MFBPCR"\AS.seek 0.71ml F*X' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*G' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*$' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*% n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*~ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*J% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*4' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %  ."MFBPCR"\AS.seek 0.604ml F*8'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*h% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*\ % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F**% h % ."MFBPCR"\AS.seek 0.58ml F*H' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +21 h F*z% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*W' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*P n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*!  +21 h  ."MFBPCR"\AS.seek 0ml F*:'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *m'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*r  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *5' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*F n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *}'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*& n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +202 h 'R ."MFBPCR"\SPE.coast F*,  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*`'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*k n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *e' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*g I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*A  +64 h  F*-% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *"'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*w n +65 h n F*/% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Y I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*D  +43 h  F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*T  +64 h  F*b% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *;'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*]% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*d I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *+'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*<  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *(': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *s'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*_ n +151 h n ."MFBPCR"\AS.seek 0ml F*2'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*>  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*O% h % ."MFBPCR"\AS.seek 0.96ml F*a'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*R' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*x% h % ."MFBPCR"\AS.seek 0.97ml F*N'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*o I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *^'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *v'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +43 h  F*7% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *I' +9988 h ' ."MFBPCR"\ATV.dial AIV F*i I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*E'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*B% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +64 h  F*l% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *6'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*C'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*9% h %m ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*, n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *}'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*) % +108 h % ."MFBPCR"\AS.seek 0ml F*k'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + C'5 P%5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990431 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '  h  P% %` h  F* *g h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ C' 2\ P% 0\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *e  h  .t\SPE.configure SPEconfig F*\ 2 *|% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'M& h & .t\CC.configure CCconfig S)#.0X( +345 h ' +13 (  .t\Forearm.seek :retract M&'\ +7 h '\  .t\Elbow.seek :hammer M&Z(M + h '4 +91 (M .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &2' +91 h ' M&L% h % .t\Forearm.seek :retract M&O'\ +91 h '\  M&@% h %\  P%.& h &2 C'& ."openPC"\PC.reconfigure PCconfig h &3 ."openPC"\PC.home.seek :open .t\CC.reconfigure CCconfig P%$x2 ^22b %a( .t\CC.home.seek :open C'$x2 ^22b ' ( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 14:00:15 began in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:120\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:06:39CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #10) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'x& h & .tHand.open M&R1 +150 h 1 M&o& h &M M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &'(- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &v'η +91 h 'η .tHand.close M&j/ +150 h / M&c& h &- M&7% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'B(W +416 h (W C'& h &W .tHand.open M&E1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C't& h &W C'% h %4 .t\CSV.dial CSR C'G  +150 h  C'%% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '$% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '~'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'J& h &W C'% h %4 .t\CSV.dial :flush C'\\  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l '  h +75 .t\CTV.dial :intake C'6  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'y'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'4  +75 h #t\Flushing upper manifold C'3& h &W C'h% h %4 .t\CSV.dial :flush C'8  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'0 +37 h .t\CS.seek 0ml C'*'4 +507 h '4 C'& h &W C'q% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C'C'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'H% h %0 .t\CS.seek 5ml C'z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'W'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&:& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +7 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&F' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C',( +416 h ( M&&& h &ӷ M&)% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &p(- +264 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&f1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:06:44CEST30-Sep-18\\n{1 => 22, 2 => 5, 3 => 17, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:06:39CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*u% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'Q& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&% F* h %\  A( % h %X A(`. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (g( +6 F*e% h %&K ."readyMFB"\ATV.dial :waste2 F*| % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (M& h & M&n& h &- M&A% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &-( +166 h ( M& & h & A(w& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &/' +90 h ' A("& h & .t\SE.seek 15.71 Pucks below Top Plate A(Y'L +106 h 'L A(& h & M&S& h & .t\Elbow.seek 3 M&D( +7 h ( M&=& h & A(T& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(b'X M&]& h & M&;% h %B .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M&_(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +126 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &@' +91 h ' .t\CC.seek :closed C'.(W +416 h (W .tHand.open M&1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'& h &W ."airStroke"\CSV.dial :waste S)2% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'x'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +150 h  C' % h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'w' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '"% h %  .t\CS.seek 4.5ml C'p'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'  +38 h  .t\CS.seek 1.275ml C'S' +919 h ' #tHeating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'=   'T9 h 9d +100 C'k9 h 9d +100 C'9 h 91d +100 C']9 h 9J d +100 C';9 h 9bd +100 C's9 h 9zd +100 C'-9 h 9d +100 C'/9 h 9d +100 C'<9 h 9 d +100 C'L9 h 9$d +100 C'9 h 9(d +100 C'Y9 h 9 ,d +100 C'D9 h 9$0d +100 C'b9 h 9<4d +100 C' 9 h 9S8d +100 C',9 h 9kd +100 C''9 h 9Td +100 C'9 h 9kd +100 C' 9 h 9d +100 C'9 h 9d +100 C'E9 h 9d +100 C'c9 h 9d +100 C'N9 h 9d +100 C't9 h 9d +100 C'9 h 9 d +100 C'G9 h 9 d +100 C'I9 h 96d +100 C'9 h 9Ld +100 C'79 h 9bd +100 C'$9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'%9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'\\9 h 9d +100 C'9 h 9d +100 C'J9 h 9(d +100 C'69 h 9>d +100 C' 9 h 9Td +100 C'l9 h 9id +100 C'9 h 9d +100 C'K9 h 9d +100 C'39 h 9d +100 C'h9 h 9d +100 C'y9 h 9d +100 C'9 h 9d +100 C'49 h 9  d +100 C'?9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'89 h 9 Td +100 C'\ 9 h 9 j d +100 C'09 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'q9 h 9 0d +100 C'*9 h 9 4d +100 C'{9 h 9 8d +100 C'H9 h 9 9 h 9 d +100 C' 9 h 9 d .t\CH.hold 5:00,85C C'   P h  +30000  C'"9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'w'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'p @ +37 h @ .t\CS.seek 5ml C'T'p +1373 h 'p .t\CSV.dial CTV C'k  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C']  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'; @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C's'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'- +113 h .t\CBV.dial CSV C'/  +19 h C'<% h %4 .t\CS.seek 5ml C'L'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C'Y% h %p .t\CS.seek 7ml C'D'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'b @ +37 h @ .t\CS.seek 10ml C' ' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C', ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&U% h %\  A(% h %X A(2. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (o( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (@& h &  M&g& h &ӷ M&W% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&^& h & A(d& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(& h &  .t\SE.seek 3.71 Pucks below Top Plate A(Z' +224 h ' M&j& h & A(R& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h '  .t\Elbow.hold M&O, +7 h , .t\Elbow.reconfigure TightElbow A(v- M&$&.,(\ #b h -+ .t\SE.seek 4.04 Pucks below Top Plate A(i' +26 h ' M&'% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M& '8 +21 h '8 M&& h & A(E& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +22 h ' .t\SE.seek 5.04 Pucks below Top Plate A(N' +36 h ' M&t& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +89 h '\  .t\Elbow.seek CC M&I(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.seek CC M&$' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(%& h &  A(~& h &  M&& h &ӷ M&\\% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &J( +142 h ( M&6& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &l' +93 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &K1 +150 h 1 M&3% h %  A(h& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&y'8 +20 h '8 M&& h & A(4& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &?' +23 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (8% h % M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C'q ` +19 h ` .t\AIV.dial :bypass C'* { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&H1 +2 h 1 M&C& h & M&z% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&9/ +150 h / M&m& h &- M&5% h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +264 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &)' +91 h ' .t\CC.seek :closed C'1(W +416 h (W C'B& h &W .tHand.open M +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%F +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%}' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%[' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Q' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'`  h +75 .t\PSV.dial :mixloop P%e +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%M  h .t\PS.seek 0ml P%|' +2610 h ' .t\PSV.dial :air P%A  +37 h  .t\PS.seek 2.5ml P%n' +2610 h ' .t\PSV.dial :mixloop P%> +38 h .t\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :mixloop P%S +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&p& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +7 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&]' +90 h ' .tHand.close M&;/ +150 h / .t\CC.seek :open C'T( +416 h ( M&-& h &ӷ M&/% h % .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &s(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&b1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C',& h & M&% h %\  A(U% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (2( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (@& h & M&g& h &- M&W% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &^( +166 h ( M&d& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &_' +89 h ' A((& h & .t\SE.seek 14.71 Pucks below Top Plate A(j'] +116 h '] A(R& h & M&v& h & .t\Elbow.seek 3 M&'( +7 h ( M&& h & A(& h & .t\Forearm.seek 3 + 300 counts M&E'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &t/ +150 h / ."downElevator"\SE.seek :down A('X M&x& h & M&% h %B .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'i(W +416 h (W .tHand.open M&c1 +150 h 1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'G  +151 h  .t\CSV.dial CTV C'.  +19 h  .t\CS.seek 5ml C'%'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'~' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%\\  +38 h  .t\PS.seek 2.5ml P%6' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%$  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%I +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%K  +151 h  .t\PS.seek :empty P%3' +2610 h ' .t\CBV.dial :waste1 C'h @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'4 @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'J @ +38 h @ .t\CS.seek 10ml C'8' +2647 h ' .t\CSV.dial CTV C'\  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'q @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'*  +150 h C'{& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&l% h %\  A(H% h %X A(C. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (z( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (y& h &  M&N& h &ӷ M&?% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &!( +142 h ( M&P& h & A(o& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 4.71 Pucks below Top Plate A(' +215 h ' M&9& h & A(m& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &5' +25 h '  .t\Elbow.hold M&0, +7 h , .t\Elbow.reconfigure TightElbow A(:- M&$&.,(\ #b h -+ .t\SE.seek 5.04 Pucks below Top Plate A(X' +27 h ' M&&% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&V'8 +21 h '8 M&& h & A(B& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &#' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(' +36 h ' M&F& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +90 h '\  .t\Elbow.seek CC M&u(ӷ +142 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&[' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'`( +416 h ( A(e& h &  A(M& h &  M&Q& h &ӷ M&A% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &>( +142 h ( M&n& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b & ' +93 h '  .t\Elbow.hold M&S, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &"1 +150 h 1 M&p% h %  A(k& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'8 +21 h '8 M&;& h & A(r& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(1% h % ."downElevator"\SE.seek :down A(-'X +100 (/% h % M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%Y  + A(6 +112 h  P%w% h % .t\PS.seek 0.499ml P%b' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%= +38 h .t\PRV.dial :air P%<  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%,  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%U  +19 h  .t\PRV.dial :air P%  +19 h  P%@% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %g' +250 h ' .t\PRV.dial :waste2 P%W @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %L' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'd& h & .tHand.open M&1 +2 h 1 M&(& h & M&s% h %\  .t\Elbow.seek FlushPuck::Garage M&R(- +166 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&D/ +150 h / M&t& h &- M&x% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +264 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' .t\CC.seek :closed C'j(W +416 h (W C'E& h &W .tHand.open M&'1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  C'T& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%O  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%G  +75 h  .tdelay 2 seconds +200 P%.% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%2' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%%' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%~ +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%\\  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb % 'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%$  +76 h .tdelay 2 seconds +200 P%I% h %r .t\PS.seek 0.475ml P%K' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%6  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%h'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%4  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%J @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%\  +75 h  .t\PS.seek 2.5ml P%8' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%*' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%{  +75 h  .t\PS.seek 2.5ml P%l' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%H +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%C  +75 h P%y% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %N' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'? & +50 h & .t\AWV.select :waste C' +7 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %o'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%9' +2610 h ' .t\PSV.dial :PML P%m +38 h .t\PS.seek 0ml P%:' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '&& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *B  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%F +21 h  ."MFBPCR"delay 20 seconds +92 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*! I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *5'6 +66 .t\PSV.dial :waste1 P%}  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%V +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%X' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%# +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%0' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%) +21 h  F*i% h %6 ."MFBPCR"\AS.seek 0.329ml F*e'ͯ +92 h P%M% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Q' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*A % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%[' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*u  +22 h  F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *p' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%k +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%;' +217 ."MFBPCR"\ATV.dial :AIV F*r I +42 h I F*1% h % ."MFBPCR"\AS.seek 0.469ml F*|' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%> +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P%] +113 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%Y' +739 h ' ."MFBPCR"delay 5 seconds +500 F*w9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*-% h % ."MFBPCR"\AS.seek 0.041ml F*z'% +336 h ' .t\PSV.dial PSR P%3 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%=' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%< +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%,' +2610 h ' .t\PSV.dial PSR P% +112 h P%U% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%@  +38 h  .t\PS.seek 0ml P%b' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%W +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%g' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %('k +2679 h 'k .t\PSV.dial :waste1 P%s  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %v' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *S'V +850 h ' .t\PSV.dial :air P%D  +112 h  .t\PS.seek 2.5ml P%t' +2610 h ' .t\PSV.dial :mixloop P%x +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %_' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*` I +43 h I F*E% h %Q ."MFBPCR"\AS.seek 0.01ml F*'' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %T' +619 h ' ."MFBPCR"\ATV.dial :waste F*c n +129 h n ."MFBPCR"\AS.seek :empty F*7'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*O  +86 h  F*G% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *.' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*L  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'2  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'%  '$  +37 h  +113  .t\CBV.dial :waste1 C'I @ +38 h @ .t\CS.seek 0ml C' '4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*6  +22 h ."MFBPCR"delay 3 seconds +300 F*K% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'4  +38 h  C'h% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*J  +22 h F*% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*\ '& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*q% h %& ."MFBPCR"\AS.seek 0.092ml F*8'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'{' +1626 h ' ."connect"\CSV.dial CTV C'*  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'H'4 +2647 h '4 C'C& h &W #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +7 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&N' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'o( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &9(- +264 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&F1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'!& h & M&% h %\  A(}% h %X A(+. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (V& h & M&& h &- M&#% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &X( +166 h ( M& & h & A(i& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &)' +90 h ' A(M& h & ."WCR"\SE.seek 13.71 Pucks below Top Plate A(5'n +125 h 'n A(0& h & M&A& h & ."WCR"\Elbow.seek 3 M&e( +7 h ( M&& h & A(u& h & ."WCR"\Forearm.seek 3 + 300 counts M&"'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Q/ +129 h '&0 ."MFBPCR"delay 3 seconds +21 h / ."downElevator"\SE.seek :down M&n& h & A(k'X M&r% h %B ."WCR"\Forearm.seek :retract M&1'\ +91 h '\  ."WCR"\Elbow.seek CC M&[(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&p& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +46 ."MFBPCR"\ATV.dial :waste2 F*] % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +2 h ' ."WCR"\CC.seek :closed C'/(W +416 h (W ."WCR"Hand.open M&1 +141 h 'R ."MFBPCR"\ATV.dial :air2 F*  +9 h 1 M&w% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +13 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *-' +78 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)|+ +7 h + S)3% h % C'Y& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'<  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."MFBPCR"\ASV.dial :wasteR F*"% h %  ."MFBPCR"\AS.seek 0.09ml F*5' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*]  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*e I +86 h I F*k% h % ."MFBPCR"\AS.seek 0.12ml F*1' +625 h ' ."MFBPCR"\ATV.dial :air3 F*8 % +21 h % F*o% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*3' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h % ."MFBPCR"\AS.seek 0.255ml F*;'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*|' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*U  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*= @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*z I +85 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*W'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*s  +43 h  #"MFBPCR"\Swish #1 of 2 F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*d% h %\  ."MFBPCR"\AS.seek 0.5ml F*D'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*g% h %(A ."MFBPCR"\AS.seek 0.6ml F*t'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *v' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*x % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +3472 h 'V ."MFBPCR"\SPE.coast F*`  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1015h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*E  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*R'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*- n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*'  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*L  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *7'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*.  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*~% h %Fj ."MFBPCR"\AS.seek 0.524ml F*2' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*\\  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*I % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*\  +65 h ."MFBPCR"\ARV.dial :air1 F*q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226087 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'?  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'C @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'^ @ +75 h @ +50 S)P% h % ."WCR"\CBV.dial between CSV and waste2b C'j  +95 h  C'% h % ."WCR"\CSV.dial between air and CTV C'Z ` +18 h ` ."WCR"\CS.seek 7.288ml C'N' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'&  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'l'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'F  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'm  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'+' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'! @ +75 h @ +50 S)}% h % ."WCR"\CBV.dial between CSV and waste2b C'#  +94 h  C'B% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'M  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'A  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '9' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'u  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C':  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'Q' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'X @ +75 h @ +50 S))% h % ."WCR"\CBV.dial between CSV and waste2b C'r  +94 h  C'>% h % ."WCR"\CSV.dial between air and CTV C'" ` +57 h ` ."WCR"\CS.seek 7.288ml C'p' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C']  'e  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'k'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'5  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '8' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'o  +150 h C'1& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&w% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (Y( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (<& h &  M&3& h &ӷ M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&;& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +89 h ' A(|& h &  ."WCR"\SE.seek 5.71 Pucks below Top Plate A(@' +205 h ' M&,& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &z' +26 h '  ."WCR"\Elbow.hold M&b, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b ."WCR"\SE.seek 6.04 Pucks below Top Plate A(s' +27 h ' M&(% h %  A(W& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&d'8 +20 h '8 M&& h & A(g& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &D' +23 h ' ."WCR"\SE.seek 7.04 Pucks below Top Plate A(' +36 h ' M&t& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&x(ӷ +143 h (ӷ M&v& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ ."WCR"\Forearm.seek CC M&E' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(S& h &  A(R& h &  M&-& h &ӷ M&c% h % ."WCR"\Forearm.seek :retract M&O'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &'( +142 h ( M&L& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &G' +93 h '  ."WCR"\Elbow.hold M&., +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &~1 +150 h 1 M&7% h %  A(\\& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&%'8 +20 h '8 M&2& h & A(I& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(K'X +100 (4% h % M&$% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +90 h '\  + 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&h1 +2 A(6 h 1 M&& h & M&J% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.seek :garage M&q'η +91 h 'η .tHand.close M&*/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +265 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C'P(W +415 h (W C'j& h &W .tHand.open M&1 +150 h 1 M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'&& h &W C'% h % .t\CSV.dial CSR C'N  +38 h  C'F% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'm% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'+& h &W C'!% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '#'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +38 h @ C'% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'M  +37 h  +113  .t\CBV.dial :waste1 C'i @ +75 h @ .t\CS.seek 0ml C'A'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'0& h &W C'u% h %4 .t\CSV.dial :flush C':  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'Q% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C')  +37 h  .t\CBV.dial :waste2b C'r  +76 h  .t\CS.seek 0ml C'>'4 +765 h '4 #t\Flushing upper manifold C'"& h &W C'n% h %4 .t\CSV.dial :flush C'e  +76 h  .t\CS.seek 5ml C']'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +38 h @ C'5% h %p .t\CS.seek 7ml C'k'i +416 h 'i ."connect"\CSV.dial CBV C'o .t\CBV.dial CSV C'1  +37 h +76 .t\CTV.dial :air C'w  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'< +37 h .t\CS.seek 0ml C'3'4 +507 h '4 C' & h &W C';% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'|'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C',% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'( +75 h .t\CS.seek 0ml C'W'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'g% h %4 .t\CSV.dial CSR C't  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'@ .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'z  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'v' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'`  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'd'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C's  +113 h  C'D% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'/  +37 h  +113  .t\CBV.dial :waste1 C'S @ +38 h @ .t\CS.seek 0ml C'R'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'-  +37 h  C'c% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'L'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'~'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'7  +38 h  C'\\% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '2'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'O  +38 h +75 .t\CTV.dial :air C''  +151 h .t\CS.seek 0ml C'4'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'$'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'h'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'J% h %4 .t\CSV.dial :flush C'G  +38 h  .t\CS.seek 5ml C'%'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'\ 'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'K'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Y'0 +507 h '0 .t\CSV.dial :waste C'. +38 h .t\CS.seek 0ml C'*'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.seek 3ml C'q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +38 h @ C'H% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'y'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'j +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'Z& h &W C'&% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'N'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +37 h @ C'^% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CTV C'm  .t\CTV.dial CSV C'l  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'+& h &W C'!% h %4 .t\CSV.dial :flush C'}  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +38 h @ C'% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'M  +76 h  .t\CS.seek 0ml C'i'4 +766 h '4 #tDisinfecting external Intake with kill C'V& h &W C'0% h %4 .t\CSV.dial CSR C'u  +37 h  C':% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'A'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'9' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q '  +37 h +76 .t\CTV.dial :intake C')  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'r' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'X  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '"'4 +607 h '4 .t\CSV.dial CSR C'n  +112 h  C'e% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'>'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'p' +1373 h ' .t\CSV.dial CBV C'5 +112 h .t\CS.seek 0ml C']'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&1& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +6 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:08:25CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST11-Oct-18" +263688 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1015h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*)% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*r  +65 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*,'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1017h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*s  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*t'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*-  +22 h ."MFBPCR"delay 2 seconds +200 F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *R'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*L  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*d% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*x  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.536ml F*2'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +108 h  ."MFBPCR"delay 7 seconds +700 F*\\% h %< ."MFBPCR"\AS.seek 0.594ml F*''\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*I n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*J' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*4  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1017h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*\  +21 h ."MFBPCR"\ATV.dial :air3 F*Y % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*&  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*F% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *v'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +21 h F*#% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*0  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1020h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*e  +10 h  F*"% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*p n +129 h n ."MFBPCR"\AS.reconfigure ASnorm F*#,,'T20 b ."MFBPCR"\AS.seek :empty F*1'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*[ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.53ml F*o' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*g n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*3  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*s'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1020h.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*`  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*x'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +21 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*~  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +65 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*4 % +87 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*%% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*G'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F*Y% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1022h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*F  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*v% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*l I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*f' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*M  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*0  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*V n +21 h n ."MFBPCR"delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.6ml F*A'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*:  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*X  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*r% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CEST1539210593.72 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1022h.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F*5 % +86 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*P'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*@  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*z% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*s'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1100h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*O  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*\\% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*4  +21 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*J I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*%% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*= % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.536ml F*G'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*K  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*.'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*6'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*H  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*F'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*v % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1100h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*b  +151 h F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +423 h ' ."MFBPCR"\ATV.dial :ASV F*Z n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*}  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*l'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*! n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*{'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*f % +43 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *#' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*V' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h % H ."MFBPCR"\AS.seek 0.735ml F*:' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +21 h F*A% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*X n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*r% h % zG ."MFBPCR"\AS.seek 0.365ml F*)' +7161 h ' ."MFBPCR"\ATV.dial :waste F*e n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*n % +43 h % F*9% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*1% h % ."MFBPCR"\AS.seek 0.579ml F*>' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +21 h % ."MFBPCR"delay 3 seconds +300 F*w% h %  ."MFBPCR"\AS.seek 0.604ml F*<'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*[ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*;  +22 h  ."MFBPCR"\AS.seek 0ml F*P'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*o % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*g' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*@'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*|' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*U n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*z  +21 h  ."MFBPCR"\AS.seek 0ml F*3'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*D  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *`' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *s'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +202 h 'R ."MFBPCR"\SPE.coast F*c  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*/'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*- n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*d  +42 h  F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *L' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*E I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*~  +42 h  F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*\\  +64 h  F*7% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *2'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*I% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *'' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *%': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*=% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*\ % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Y' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *K'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *G': +3042 h ': ."MFBPCR"\ATV.dial :ASV F** n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*. n +150 h n ."MFBPCR"\AS.seek 0ml F*H'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*6 % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*y% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*F' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*m% h % ."MFBPCR"\AS.seek 0.97ml F*N'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*Z I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *!'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *8'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*f% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *{' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*#' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*0% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*u  +21 h ."MFBPCR"\ATV.dial :air3 F*V % +43 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*X% h %Z ."MFBPCR"\AS.seek 0.81ml F*r'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*9% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*1  +64 h  F*p% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*W  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *s'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*S % +108 h % ."MFBPCR"\AS.seek 0ml F*-'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast .\PC.coast C'6 P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +3562845 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )d h  C' 'R h  P% %/ h  F* *E h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *L  h  .t\SPE.configure SPEconfig F*\ 2 *]% h %V .t\AS.configure ASconfig S)~% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'O& S)#.0X 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:09:24CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #11) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'm& h & .tHand.open M&F1 +150 h 1 M&Z& h &M M&N% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &}(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &!'η +91 h 'η .tHand.close M&l/ +150 h / M&& h &- M&f% h %η .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'0(W +416 h (W C'#& h &W .tHand.open M& 1 +150 h 1 M&u% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Q& h &W C'i% h %4 .t\CSV.dial CSR C':  +150 h  C'X% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'A'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'e'[ +463 h '[ .t\CSV.dial :waste C'r +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +2009 h '4 #t\Purging external Intake C'9& h &W C')% h %4 .t\CSV.dial :flush C'5  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'" 'p  h +75 .t\CTV.dial :intake C'?  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'w'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'>  +75 h #t\Flushing upper manifold C' & h &W C' % h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C';'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +37 h @ C'P% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'o +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C',& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'g% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'@'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +507 h '0 .t\CSV.dial :waste C'z +75 h .t\CS.seek 0ml C'|'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&3& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +7 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&`/ +150 h / .t\CC.seek :open C'c( +416 h ( M&& h &ӷ M&S% h % .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &a'η +91 h 'η .tHand.open M&j1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:09:29CEST30-Sep-18\\n{1 => 22, 2 => 8, 3 => 14, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:09:24CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*B% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&d% h %\  A(R% h %X A(/. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (E( +6 F*L% h %&K ."readyMFB"\ATV.dial :waste2 F*] % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *~' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (O& h & M&x& h &- M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &7( +166 h ( M& & h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &I' +90 h ' A(2& h & .t\SE.seek 12.71 Pucks below Top Plate A( ' +136 h ' A('& h & M&4& h & .t\Elbow.seek 3 M&$( +7 h ( M&J& h & A(& h & .t\Forearm.seek 3 + 300 counts M&='B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&\ & A(h'X h & M&% h %B .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.seek CC M&.(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C'v(W +416 h (W .tHand.open M&1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)6+ +7 h + S)H% h % C'm& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +75 h @ ."airStroke"\CS.seek 10ml C' ' +144 h  .t\CTV.dial :air C'  +75 h .tdelay 15 seconds +691 h ' +809 .t\SSV.dial :spit,avoiding: :exhaust S)< +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'w  .t\SS.seek 0ml S)k' +6 h ' .t\SSV.dial CBV,avoiding: :exhaust S)P  +144 h  +7  S);% h % .t\CBV.dial between SSV and waste2 C'o  +19 h  C'[% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'  +37 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +2610 h '4 C'% h %4 S),% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C' @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X9 h 9d +100 C'19 h 9'd +100 C'|9 h 9>d +100 C' 9 h 9Td +100 C'?9 h 9kd +100 C'<9 h 9d +100 C'w9 h 9d +100 C'P9 h 9d +100 C';9 h 9d +100 C'o9 h 9d +100 C'[9 h 9d +100 C'9 h 9 d +100 C'n9 h 9 d +100 C' 9 h 96d +100 C'9 h 9Ld +100 C',9 h 9bd +100 C'9 h 9xd +100 C'k9 h 9d +100 C'9 h 9d +100 C'W9 h 9d +100 C'g9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'@9 h 9d +100 C'z9 h 9(d +100 C'39 h 9>d +100 C'9 h 9Td +100 C't9 h 9id +100 C'9 h 9d +100 C'D9 h 9d +100 C'U9 h 9d +100 C'`9 h 9d +100 C'V9 h 9d +100 C'09 h 9d +100 C'S9 h 9  d +100 C'9 h 9 d +100 C'M9 h 9 *d +100 C'T9 h 9 ?d +100 C'^9 h 9 Td +100 C'j9 h 9 j d +100 C'9 h 9 $d +100 C'(9 h 9 (d +100 C's9 h 9 ,d +100 C'B9 h 9 0d +100 C'd9 h 9 4d +100 C'R9 h 9 8d +100 C'/9 h 9 , +7 h , .t\Elbow.reconfigure TightElbow A(1- h -+ M&$&.,(\ #b .t\SE.seek 7.04 Pucks below Top Plate A(|' +27 h ' M& % h %  A(?& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&<'8 +21 h '8 M&w& h & A(P& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &;' +22 h ' .t\SE.seek 8.04 Pucks below Top Plate A(o'w +36 h 'w M&[& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +90 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'( +416 h ( A(W& h &  A(g& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +142 h ( M&3& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &t' +93 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &D1 +150 h 1 M&U% h %  A(`& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&V'8 +21 h '8 M&0& h & A(S& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(M% h %w ."downElevator"\SE.seek :down A(T'X +100 (^% h % M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +81 h 'X ."downElevator"\SE.coast A(5 +8 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'( @ +19 h @ .t\CS.seek 10ml C's' +7 h ' .t\CSV.dial between air and CTV C'B ` +18 h ` .t\AIV.dial :bypass C'd { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&/1 +2 h 1 M&L& h & M&]% h %\  .t\Elbow.seek FlushPuck::Garage M&-(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&a'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &J' +90 h ' .t\CC.seek :closed C'_(W +416 h (W C''& h &W .tHand.open M&1 +150 h 1 M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%\ % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%7' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%=  C'  h +75 .t\PSV.dial :mixloop P%$ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%Y  h .t\PS.seek 0ml P%h' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%c' +2609 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.seek 0ml P%m' +2610 h ' .t\PSV.dial :air P%%  +37 h  .t\PS.seek 2.5ml P%E' +2610 h ' .t\PSV.dial :mixloop P%N +38 h .t\PS.seek 0ml P%6' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &q(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&F/ +150 h / .t\CC.seek :open C'( +416 h ( M&f& h &ӷ M&y% h % .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&b1 +150 h 1 M&Z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'u& h & M&Q% h %\  A(i% h %X A(:. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (#( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &r( +166 h ( M&e& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &.' +90 h ' A(X& h & .t\SE.seek 11.71 Pucks below Top Plate A(p'Ɛ +145 h 'Ɛ A(G& h & M&1& h & .t\Elbow.seek 3 M& ( +7 h ( M&?& h & A(w& h & .t\Forearm.seek 3 + 300 counts M&P'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &[/ +150 h / ."downElevator"\SE.seek :down M&9& A('X h & M&A% h %B .t\Forearm.seek :retract M&5'\ +91 h '\  .t\Elbow.seek CC M&"(ӷ +55 h 'X ."downElevator"\SE.coast A(5 +87 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'|(W +416 h (W .tHand.open M&;1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'n  +151 h  .t\CSV.dial CTV C'v  +19 h  .t\CS.seek 5ml C'W'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'g' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%D  +151 h  .t\PS.seek :empty P%U' +2610 h ' .t\CBV.dial :waste1 C'` @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'0  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'S @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'M  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'@ ` +19 h ` .t\CSV.dial :air C'z @ +38 h @ .t\CS.seek 10ml C'^' +2647 h ' .t\CSV.dial CTV C'j  +38 h  .t\CS.seek 0ml C'('4 +2647 h '4 .t\CBV.dial :waste1 C'B @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'd  +150 h C'R& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&/% h %\  A(L% h %X A(]. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (t( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (V& h &  M&o& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &x( +143 h ( M&\\& h & A(T& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(!& h &  .t\SE.seek 7.71 Pucks below Top Plate A(' +185 h ' M&& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h '  .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(a- h -+ M&$&.,(\ #b .t\SE.seek 8.04 Pucks below Top Plate A('w +27 h 'w M&4% h %  A(-& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&2'8 +21 h '8 M&& h & A('& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 9.04 Pucks below Top Plate A(O'f +36 h 'f M&& h & M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +90 h '\  .t\Elbow.seek CC M&I(ӷ +142 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C' ( +416 h ( A($& h &  A(Y& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &H( +143 h ( M&c& h & A(%& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &m' +92 h '  .t\Elbow.hold M&N, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &E1 +150 h 1 M&*% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&+'8 +21 h '8 M&F& h & A(C& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(_% h %f ."downElevator"\SE.seek :down A(f'X +100 (y% h % M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +72 h 'X ."downElevator"\SE.coast A(5 +17 h '\  .t\SC.coast .\PSV.dial :air A(6 P%}  +113 h  P%q% h % .t\PS.seek 0.499ml P%b' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%Z +38 h .t\PRV.dial :air P%l  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%u  +38 h .t\CBV.dial AIV C'Q  +38 h .t\PRV.dial between CBV and air P%i  +18 h  .t\PRV.dial :air P%:  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&)1 +2 h 1 M&X& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&G(- +166 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &- .t\Forearm.seek :garage M&w'η +91 h 'η .tHand.close M&8/ +150 h / M&[& h &- M&9% h %η .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +264 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'p& h &W .tHand.open M& 1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%; @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%>  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%n  +76 h  .tdelay 2 seconds +200 P%v% h % .t\PS.seek 1.499ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%#' +1094 h ' .t\PSV.dial :air P%s  +37 h  .t\PS.seek 0ml P%W' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%g +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%6% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P% % h %r .t\PS.seek 0.475ml P%D' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%`'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%0  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%S  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %M' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%@ +75 h .t\PTV.dial :PRV,avoiding: :puck P%z @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%j  +75 h  .t\PS.seek 2.5ml P%^' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%B +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%d' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%R  +75 h  .t\PS.seek 2.5ml P%/' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%L +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%]  +75 h P%V% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %o' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%\\% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %T'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'! { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %"' +1312 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :PML P%k +37 h .t\PS.seek 0ml P%a' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '4& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'-& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F* @@T .t\AIV.dial :bypass F*'  .t\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%\ % h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%7  +38 h  .t\PS.seek 0ml P%&' +1597 h ' .t\PSV.dial PSR P%2 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*=  +22 h ' .t\PSV.dial PSR P%J +21 h  F*|% h %6 ."MFBPCR"\AS.seek 0.329ml F*$'ͯ +92 h P%Y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*I  +21 h  F*E% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b **' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%F' +217 ."MFBPCR"\ATV.dial :AIV F*C I +43 h I F*_% h % ."MFBPCR"\AS.seek 0.469ml F*h' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial PSR P%~ +113 h P%m% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%+' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%O  +38 h  .t\PS.seek 0ml P%}' +739 h ' ."MFBPCR"delay 5 seconds +500 F*q9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f% h % ."MFBPCR"\AS.seek 0.041ml F*t'% +336 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Z' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%l +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%u' +2609 h ' .t\PSV.dial PSR P%Q +113 h P%i% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%:' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%b' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%) +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %X'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %1' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*? I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *N'V +849 h ' .t\PSV.dial :air P%8  +113 h  .t\PS.seek 2.5ml P%[' +2610 h ' .t\PSV.dial :mixloop P%9 +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %{' +5106 h ' .t\PSV.dial :air P%w  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %.' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *A'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*p% h %Q ."MFBPCR"\AS.seek 0.01ml F* ' +170 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.seek :empty F*,'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*>  +86 h  F*n% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *v' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*<% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *s'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'#  +37 h  C'g% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'6' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'W  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +22 h ."MFBPCR"delay 3 seconds +300 F*D% h %_ ."MFBPCR"\AS.seek 0.434ml F*0'ݏ +110 h '4 .t\CSV.dial CSR C'S  +37 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'@'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*z  +22 h F*M% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*j'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F*B% h %& ."MFBPCR"\AS.seek 0.092ml F*^'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'R' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'L'4 +2647 h '4 C']& h &W #tGaraging FlushPuck in Collection Clamp M&V& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &/(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&o' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'T( +416 h ( M&& h &ӷ M&"% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &''η +91 h 'η .tHand.open M&1 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'x& h & M&% h %\  A(7% h %X A(K. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( ( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (2& h & M&&& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&=& h & A(|& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(Y& h & ."WCR"\SE.seek 10.71 Pucks below Top Plate A(' +155 h ' A(& h & M&& h & ."WCR"\Elbow.seek 3 M&$( +7 h ( M&%& h & A(I& h & ."WCR"\Forearm.seek 3 + 300 counts M&E'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +100 h '&0 ."MFBPCR"delay 3 seconds +50 h / ."downElevator"\SE.seek :down A('X M&c& h & M&C% h %B ."WCR"\Forearm.seek :retract M&_'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&*& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +16 ."MFBPCR"\ATV.dial :waste2 F*~ % +42 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +33 h ' ."WCR"\CC.seek :closed C'y(W +415 h (W ."WCR"Hand.open M&O1 +112 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *q' +16 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)h+ +7 h + S)U% h % C'}& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'l  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +64 h  F*n% h % ."MFBPCR"\AS.seek 0.52ml F*,' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteR F*<  +21 h  ."MFBPCR"\AS.seek 0.3ml F*v': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*#'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*g % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *W'N +1026 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +43 h I F*6% h %N ."MFBPCR"\AS.seek 0.109ml F*3'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*D n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*S  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *`% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *s' ?x +3301 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *z' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*M% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *0' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*B'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*j n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *@'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*d % +43 h % F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *]'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*V I +22 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F*\\'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*! % +21 h % F*r% h %H ."MFBPCR"\AS.seek 0.069ml F*5' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F*"' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h % q ."MFBPCR"\AS.seek 0.794ml F*-' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*\ '  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*k I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *x' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*7'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*'% h % ."MFBPCR"\AS.seek 0.579ml F*=' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*%' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*I n +86 h n ."MFBPCR"\ASV.dial :wasteR F*a  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *C'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*J% h %V ."MFBPCR"\AS.seek 0.59ml F**' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*H n +108 h n ."MFBPCR"\ASV.dial :wasteR F*~% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*$ I +85 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*_' +626 h ' ."MFBPCR"\ATV.dial :air3 F*^ % +21 h % F*T% h % ."MFBPCR"\AS.seek 0.13ml F*+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*U' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h % ."MFBPCR"\AS.seek 0.255ml F*m'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F* % h %X ."MFBPCR"\AS.seek 0.24ml F*h' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*i n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Q  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *:'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*Z @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*t I +86 h I ."MFBPCR"\AS.seek 0ml F*)'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *?'\  +1032 h '\  F*e% h %\  ."MFBPCR"\AS.seek 0.5ml F*8'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*['\  +1032 h '\  F*w% h %\  ."MFBPCR"\AS.seek 0.5ml F*{'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *1' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*9 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1117h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*p  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *>'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *,'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*v  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*g% h %Fj ."MFBPCR"\AS.seek 0.524ml F*#' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*(' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*W'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*s'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*0  +43 h  ."MFBPCR"\ASV.dial :waste1 F*j  +65 h ."MFBPCR"\ARV.dial :air1 F*B  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*d% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*V % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226116 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'] @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'r @ +75 h @ +50 S)\\% h % ."WCR"\CBV.dial between CSV and waste2b C'P  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'5 ` +19 h ` ."WCR"\CS.seek 7.288ml C'o' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'4  '"  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '/'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '-' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'\  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'K' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'x @ +75 h @ +50 S)7% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C''% h % ."WCR"\CSV.dial between air and CTV C'& ` +57 h ` ."WCR"\CS.seek 7.288ml C'|' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Y  '=  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'a  +38 h  C'%% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)J% h % ."WCR"\CBV.dial between CSV and waste2b C'C  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C'~ ` +56 h ` ."WCR"\CS.seek 7.288ml C'*' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'E  '$  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '^' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'T  +151 h C'_& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&O% h %\  A(% h %X A(+. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (}( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (l& h &  M&U& h &ӷ M&F% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +143 h ( M&m& h & A(i& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Q' +89 h ' A(h& h &  ."WCR"\SE.seek 8.71 Pucks below Top Plate A(' +175 h ' M&u& h & A(:& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &t' +26 h '  ."WCR"\Elbow.hold M&b, +6 h , ."WCR"\Elbow.reconfigure TightElbow A()- h -+ M&$&.,(\ #b ."WCR"\SE.seek 9.04 Pucks below Top Plate A('f +27 h 'f M&X% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&e'8 +20 h '8 M&?& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +22 h ' ."WCR"\SE.seek 10.04 Pucks below Top Plate A(w'U +37 h 'U M&[& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +89 h '\  ."WCR"\Elbow.seek CC M&9(ӷ +142 h (ӷ M&1& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.seek CC M&p' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'A( +416 h ( A(N& h &  A(G& h &  M&q& h &ӷ M&;% h % ."WCR"\Forearm.seek :retract M&>'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +143 h ( M&& h & A(<& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &n' +92 h '  ."WCR"\Elbow.hold M&v, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &g1 +150 h 1 M&,% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&#& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &6' +22 h ' A((% h %U ."downElevator"\SE.seek :down A(D'X +100 (S% h % M&W% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +61 h 'X ."downElevator"\SE.coast A(5 +29 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&`1 +2 h 1 M&M& h & M&z% h %\  .t\Elbow.seek FlushPuck::Garage M&s(- +166 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.seek :garage M&B'η +90 h 'η .tHand.close M&d/ +150 h / M&& h &- M&@% h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &r' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'P& h &W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'4& h &W C'"% h % .t\CSV.dial CSR C'o  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'/'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'k% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '\ '[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '-'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'K& h &W C'x% h %4 .t\CSV.dial :flush C'7  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +38 h @ C'&% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CTV C'=  .t\CTV.dial CSV C'Y  +37 h  +113  .t\CBV.dial :waste1 C'| @ +76 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'I% h %4 .t\CSV.dial :flush C'a  +75 h  .t\CS.seek 5ml C'%'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'J  +37 h  .t\CBV.dial :waste2b C'C  +76 h  .t\CS.seek 0ml C'H'4 +766 h '4 #t\Flushing upper manifold C'~& h &W C'c% h %4 .t\CSV.dial :flush C'E  +75 h  .t\CS.seek 5ml C'$'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T '_  +38 h +75 .t\CTV.dial :air C'O  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'l +38 h .t\CS.seek 0ml C'U'4 +506 h '4 C'F& h &W C'm% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 3ml C'h'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'u% h %0 .t\CS.seek 5ml C':'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C')'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +507 h '0 .t\CSV.dial :waste C'X +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'?& h &W C'% h %4 .t\CSV.dial CSR C'[  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'Q'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C't  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'1' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'e'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +112 h  C'8% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'{  'y  +37 h  +113  .t\CBV.dial :waste1 C'N @ +38 h @ .t\CS.seek 0ml C'G'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'q  +37 h  C';% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +710 h ' ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'9  +76 h  .t\CS.seek 0ml C'g'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C',  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '#'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +709 h ' ."connect"\CSV.dial CBV C'( .t\CBV.dial CSV C'>  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'S'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'`'4 +506 h '4 #t\Flushing upper manifold C'M& h &W C'z% h %4 .t\CSV.dial :flush C'n  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'0% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6 +113 h .t\CTV.dial :air .\CS.seek 0ml C'D'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'}'0 +506 h '0 .t\CSV.dial :waste C'v +38 h .t\CS.seek 0ml C'd'4 +506 h '4 C'& h &W C'@% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C'B'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'L% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'V'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C'P +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'5& h &W C'4% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 5ml C'o'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'r% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CTV C'k  .t\CTV.dial CSV C'/  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'\ '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'x% h %4 .t\CSV.dial :flush C'7  +75 h  .t\CS.seek 5ml C'-'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +38 h @ C'&% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'=  +38 h  .t\CBV.dial :waste2b C'Y  +75 h  .t\CS.seek 0ml C'|'4 +766 h '4 #tDisinfecting external Intake with kill C'2& h &W C'% h %4 .t\CSV.dial CSR C'I  +37 h  C'a% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'%  +38 h +75 .t\CTV.dial :intake C'J  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'C' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '~'4 +607 h '4 .t\CSV.dial CSR C'c  +113 h  C'E% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'*' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'$'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&_& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +6 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C'+( +416 h ( M&F& h &ӷ M&m% h % .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &U(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &h'η +91 h 'η .tHand.open M&:1 +150 h 1 M&)% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'f& h & .tHand.open M&?1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &[(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&Q'η +91 h 'η .tHand.close M&/ +150 h / M&t& h &- M& % h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &e' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'{& h &W .tHand.open M&y1 +150 h 1 M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C';% h %4 .t\CSV.dial CSR C'G  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'p'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '9% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '<'[ +463 h '[ .t\CSV.dial :waste C', +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'g& h &W C'#% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'S'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'W& h &W C'M% h %4 .t\CSV.dial :flush C'z  +75 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'w% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'6'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +506 h '0 .t\CSV.dial :waste C'D +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'}& h &W C'% h %4 .t\CSV.dial :flush C'@  +38 h  .t\CS.seek 3ml C'3'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +37 h @ C']% h %0 .t\CS.seek 5ml C'L'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +506 h '0 .t\CSV.dial :waste C'V +75 h .t\CS.seek 0ml C'P'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &5(ӷ +7 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'r( +415 h ( M&o& h &ӷ M&k% h % .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &x'η +91 h 'η .tHand.open M&71 +150 h 1 M&\ % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST12-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:11:13CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST12-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1117h1500ml.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*=  +22 h ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*%'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +64 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *E'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*l % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*u'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1119h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*8% h %V ."MFBPCR"\AS.seek 0.5ml F*['(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*+ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*N  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +21 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*e% h %Fj ."MFBPCR"\AS.seek 0.524ml F*$' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*9  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*,' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*<  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*#'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*W  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*n'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*0 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1119h1500ml.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*j  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *@'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*d% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +21 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*B'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +21 h F*s% h %n ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *1'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +31 h  ."MFBPCR"\ATV.dial :air3 F*5 % +87 h % F*7% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*='m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1121h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*E  +11 h  F*~% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F** n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*l  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*F n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*m% h % ."MFBPCR"\AS.seek 0.53ml F*T' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*: % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*i'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*U  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*X % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1121h1500ml.pcr F*[% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*{ % +86 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*9'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*$% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*S % +87 h % F*`% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*n'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *v'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V @CEST1539295218.04 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1200h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*A  +11 h  F*1% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*o n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*K  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *^'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*& n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Y  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*='< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*-% h %< ."MFBPCR"\AS.seek 0.594ml F*I'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*a  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*J' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*C% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*E  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1200h1500ml.pcr F*c% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F**  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *l'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*m% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*{% h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*+  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1202h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*<  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*#'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*( n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *M'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*z I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*Z % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.53ml F*0' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*j  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*n'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*v'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*d n +21 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*L  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.65ml F*V' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*s  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*4% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*A  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*1 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1202h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*" @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*b  +150 h F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *K' +424 h ' ."MFBPCR"\ATV.dial :ASV F*5 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*7  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*/'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*x n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *^'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *&'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*R'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*\ % +43 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*=% h % ."MFBPCR"\AS.seek 0.71ml F*2' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h % H ."MFBPCR"\AS.seek 0.735ml F*a' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*%' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*C% h % zG ."MFBPCR"\AS.seek 0.365ml F*J' +7161 h ' ."MFBPCR"\ATV.dial :waste F*E n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *~'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*c % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*_% h % ."MFBPCR"\AS.seek 0.579ml F*H' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %  ."MFBPCR"\AS.seek 0.604ml F*l'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*!% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*m  +22 h  ."MFBPCR"\AS.seek 0ml F*\\'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *u'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*T % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*i% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*h' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*Q n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*t  +21 h  ."MFBPCR"\AS.seek 0ml F*U'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *['V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*8  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*{ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*+ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *N'R +202 h 'R ."MFBPCR"\SPE.coast F*;  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*y'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*q n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*p I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*g  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *<': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*,% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *#'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*( n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*W  +43 h  F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*M  +65 h  F*Z% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *6'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*w n +65 h n F*j% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *}' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*0 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *n': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*d n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *@'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*v n +151 h n ."MFBPCR"\AS.seek 0ml F*L'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*]  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*3 % +43 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*V% h % ."MFBPCR"\AS.seek 0.96ml F*A'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*k% h % ."MFBPCR"\AS.seek 0.97ml F*o'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*5 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *x'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *^'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*&  +43 h  F*\ % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *R' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*- n +129 h n ."MFBPCR"\AS.seek 0ml F*='V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*I  +22 h ."MFBPCR"\ATV.dial :air3 F*2 % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*_  +64 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*u'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*N % +108 h % ."MFBPCR"\AS.seek 0ml F*q'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990523 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )e h  C' 'G h  P% %y h  F* *p h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ C' 2\ F*\ 9\ P% 0\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *$% h %V .t\AS.configure ASconfig S)g% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'>& S)#.0X 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:12:12CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #12) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'k& h & .tHand.open M&1 +150 h 1 M&5& h &M M&o% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &7(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &x'η +91 h 'η .tHand.close M&// +150 h / M&&& h &- M&\ % h %η .t\Forearm.seek :retract M&^'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &-' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&=1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'|% h %4 .t\CSV.dial CSR C'a  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '%% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'E'[ +463 h '[ .t\CSV.dial :waste C'C +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'c'4 +2009 h '4 #t\Purging external Intake C' & h &W C'J% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '_' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'* .t\CBV.dial CSV C'~  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'O'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'H  +75 h #t\Flushing upper manifold C'F& h &W C'!% h %4 .t\CSV.dial :flush C'l  +75 h  .t\CS.seek 5ml C'm'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'\\% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +507 h '0 .t\CSV.dial :waste C'f +37 h .t\CS.seek 0ml C'i'4 +507 h '4 C'u& h &W C'% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.seek 3ml C')'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +507 h '0 .t\CSV.dial :waste C't +75 h .t\CS.seek 0ml C'h'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +7 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C';( +416 h ( M&+& h &ӷ M&N% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &q'η +91 h 'η .tHand.open M&.1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:12:17CEST30-Sep-18\\n{1 => 22, 2 => 11, 3 => 11, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:12:12CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*P% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'"& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&e% h %\  A(G% h %X A(y. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (p( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*$ % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *g' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (>& h & M&9& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &,( +166 h ( M&<& h & A((& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(#& h & .t\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' A( & h & M&S& h & .t\Elbow.seek 3 M&W( +6 h ( M&z& h & A(Z& h & .t\Forearm.seek 3 + 300 counts M&M'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &w/ +150 h / ."downElevator"\SE.seek :down M&`& A(j'X h & M&6% h %B .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.seek CC M&v(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +68 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' +91 h ' .t\CC.seek :closed C'1(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)3+ +6 h + S)L% h % C'k& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'4 +2609 h '4 C'9% h %4 S)% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'q @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'!9 h 9Td +100 C'9 h 9kd +100 C'l9 h 9d +100 C'O9 h 9d +100 C'\\9 h 9d +100 C'm9 h 9d +100 C'T9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'c9 h 9 d +100 C'F9 h 96d +100 C':9 h 9Ld +100 C'u9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'i9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'?9 h 9d +100 C')9 h 9d +100 C'9 h 9d +100 C't9 h 9(d +100 C'U9 h 9>d +100 C' 9 h 9Td +100 C'[9 h 9id +100 C'f9 h 9d +100 C'89 h 9d +100 C'Q9 h 9d +100 C' 9 h 9d +100 C'29 h 9d +100 C'9 h 9d +100 C'N9 h 9  d +100 C'9 h 9 d +100 C'Y9 h 9 *d +100 C'{9 h 9 ?d +100 C''9 h 9 Td +100 C'.9 h 9 j d +100 C'+9 h 9 $d +100 C'X9 h 9 (d +100 C'9 h 9 ,d +100 C'P9 h 9 0d +100 C'e9 h 9 4d +100 C'G9 h 9 8d +100 C'y9 h 9 9 h 9 kd +100 C'@9 h 9 d +100 C'Z9 h 9 d +100 C'`9 h 9 d +100 C',9 h 9 d +100 C' 9 h 9 d +100 C'69 h 9 d +100 C'49 h 9 d +100 C'9 h 9 d +100 C'M9 h 9 d +100 C'9 h 9 1d +100 C'W9 h 9 Dd +100 C'}9 h 9 Xd +100 C'j9 h 9 kd +100 C'9 h 9 ~d +100 C';9 h 9 d +100 C'L9 h 9 d +100 C'k9 h 9 d .t\CH.hold 5:00,85C C'   P h  +30000  C'd9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'B'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'3 @ +113 h @ .t\CSV.dial :air C'p @ +37 h @ .t\CS.seek 5ml C'0'p +1373 h 'p .t\CSV.dial CTV C'  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'K @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'g  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'&'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'\ +113 h .t\CBV.dial CSV C'V  +19 h C'/% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'R  +38 h C'7% h %p .t\CS.seek 7ml C'^'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'b @ +37 h @ .t\CS.seek 10ml C'x' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'I ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&|% h %\  A(a% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (=( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (-& h &  M&s& h &ӷ M&%% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&C& h & A(E& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(J& h &  .t\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' M&& h & A(~& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &*' +25 h '  .t\Elbow.hold M&H, +7 h , .t\Elbow.reconfigure TightElbow A(_- h -+ M&$&.,(\ #b .t\SE.seek 10.04 Pucks below Top Plate A(h'U +27 h 'U M&!% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&l'8 +21 h '8 M&O& h & A(\\& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &m' +22 h ' .t\SE.seek 11.04 Pucks below Top Plate A(T'D +36 h 'D M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  .t\Elbow.seek CC M&F(ӷ +142 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'i( +416 h ( A(& h &  A(& h &  M&?& h &ӷ M&)% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &t( +142 h ( M&U& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &[' +93 h '  .t\Elbow.hold M&f, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &81 +150 h 1 M&Q% h %  A( & h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&2'8 +21 h '8 M&& h & A(N& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(Y% h %D ."downElevator"\SE.seek :down A({'X +100 ('% h % M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +52 h 'X ."downElevator"\SE.coast A(5 +37 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'X @ +19 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C'P ` +18 h ` .t\AIV.dial :bypass C'e { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'G& h & .tHand.open M&y1 +2 h 1 M&& h & M&$% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&q'η +91 h 'η .tHand.close M&"/ +150 h / M&<& h &- M&(% h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +90 h ' .t\CC.seek :closed C'r(W +416 h (W C' & h &W .tHand.open M&w1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%Z +151 h P%`% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%,' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%M  C'  h +75 .t\PSV.dial :mixloop P%W +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%}  h .t\PS.seek 0ml P%j' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%;' +2609 h ' .t\PSV.dial :mixloop P%L +38 h .t\PS.seek 0ml P%k' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%d' +2610 h ' .t\PSV.dial :mixloop P%o +38 h .t\PS.seek 0ml P%3' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&p& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'0( +416 h ( M&\ & h &ӷ M&V% h % .t\Forearm.seek :retract M&/'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &&(- +264 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&b1 +150 h 1 M&5% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'I& h & M&% h %\  A(|% h %X A(a. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (-& h & M&s& h &- M&%% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &C( +166 h ( M&E& h & A(J& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &v' +90 h ' A(& h & .t\SE.seek 8.71 Pucks below Top Plate A(~' +175 h ' A(_& h & M&n& h & .t\Elbow.seek 3 M&!( +6 h ( M&& h & A(O& h & .t\Forearm.seek 3 + 300 counts M&\\'B +23 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&*(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'h(W +416 h (W .tHand.open M&m1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'c  +150 h  .t\CSV.dial CTV C'1  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'?'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%)  +37 h  .t\PS.seek 2.5ml P%U' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%F +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%8  +151 h  .t\PS.seek :empty P%Q' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'N @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'Y  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C't @ +37 h @ .t\CS.seek 10ml C''' +2648 h ' .t\CSV.dial CTV C'.  +37 h  .t\CS.seek 0ml C'X'4 +2647 h '4 .t\CBV.dial :waste1 C'P @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'e  +151 h C'G& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&y% h %\  A(% h %X A($. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ([( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (2& h &  M&T& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &9( +143 h ( M&& h & A({& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &=' +89 h ' A(x& h &  .t\SE.seek 10.71 Pucks below Top Plate A(f' +155 h ' M&"& h & A(<& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &(' +25 h '  .t\Elbow.hold M&+, +7 h , .t\Elbow.reconfigure TightElbow A(q- M&$&.,(\ #b h -+ .t\SE.seek 11.04 Pucks below Top Plate A('D +27 h 'D M&S% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&#'8 +21 h '8 M&i& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &w' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(>'3 +36 h '3 M&Z& h & M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +90 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&M/ +150 h / .t\CC.seek :open C'( +416 h ( A(W& h &  A(}& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +143 h ( M&;& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &k' +92 h '  .t\Elbow.hold M&o, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &d1 +150 h 1 M&p% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&K'8 +21 h '8 M&& h & A(]& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &@' +22 h ' A(r% h %3 ."downElevator"\SE.seek :down A(\ 'X +100 (V% h % M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +43 h 'X ."downElevator"\SE.coast A(5 +46 h '\  .t\SC.coast .\PSV.dial :air A(6 P%7  +113 h  P%B% h % .t\PS.seek 0.499ml P%b' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%5 +38 h .t\PRV.dial :air P%/  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%I  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%|  +18 h  .t\PRV.dial :air P%a  +19 h  P%-% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %s' +251 h ' .t\PRV.dial :waste2 P%% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'E& h & .tHand.open M&J1 +2 h 1 M&& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&_(- +166 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&^/ +150 h / M&& h &- M& % h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +264 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'~& h &W .tHand.open M&!1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'0& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%m @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%H  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%c  +76 h  .tdelay 2 seconds +200 P%1% h % .t\PS.seek 1.499ml P%l' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%)  +37 h  .tdelay 2 seconds +200 P%3% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb % 'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%F% h %r .t\PS.seek 0.475ml P%8' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%U  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%N  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Y' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .t\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial :air P%.  +75 h  .t\PS.seek 2.5ml P%'' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%P +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%e' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%G  +75 h  .t\PS.seek 2.5ml P%y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%$  +75 h P%2% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %T' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %{'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'x { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %*' +1312 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :PML P%q +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'S& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& F*i @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%Z +22 h  ."MFBPCR"delay 20 seconds +91 h P%`% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%=' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*9 I +129 h I F*f% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *('6 +66 .t\PSV.dial :waste1 P%,  +38 h  .t\PS.seek 0ml P%4' +1597 h ' .t\PSV.dial PSR P%# +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%+' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*M  +22 h ' .t\PSV.dial PSR P%z +21 h  F*h% h %6 ."MFBPCR"\AS.seek 0.329ml F*W'ͯ +92 h P%}% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *;'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%6' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*d% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *p' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*] I +43 h I F*r% h % ."MFBPCR"\AS.seek 0.469ml F*j' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%L +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%g +113 h P%k% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%K' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*B9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*@ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*\ % h % ."MFBPCR"\AS.seek 0.041ml F*['% +336 h ' .t\PSV.dial PSR P%Q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%5' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%I' +2609 h ' .t\PSV.dial PSR P% +113 h P%|% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%-  +37 h  .t\PS.seek 0ml P%b' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%&  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %n' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *o'V +849 h ' .t\PSV.dial :air P%^  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %R' +5106 h ' .t\PSV.dial :air P%O  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %v' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*:  +43 h  F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*~% h %Q ."MFBPCR"\AS.seek 0.01ml F*!' +170 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %0' +618 h ' ."MFBPCR"\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.seek :empty F*u'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*H  +86 h  F*c% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *1' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*l% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C')'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'3' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'F @ +38 h @ .t\CS.seek 0ml C' '4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*U  +22 h ."MFBPCR"delay 3 seconds +300 F*8% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'N  +37 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*t  +22 h F*Y% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*.'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*? n +107 h n F*P% h %& ."MFBPCR"\AS.seek 0.092ml F*''&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'G' +1625 h ' ."connect"\CSV.dial CTV C'e  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'$& h &W #tGaraging FlushPuck in Collection Clamp M&2& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&x/ +150 h / .t\CC.seek :open C'{( +416 h ( M&"& h &ӷ M&*% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &<(- +264 h (- M&S& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&Z1 +150 h 1 M&`% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'9& h & M&f% h %\  A(,% h %X A(D. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (=( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (#& h & M&4& h &- M&w% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&M& h & A(h& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &z' +89 h ' A(}& h & ."WCR"\SE.seek 7.71 Pucks below Top Plate A((' +185 h ' A(+& h & M&& h & ."WCR"\Elbow.seek 3 M&W( +7 h ( M&& h & A( & h & ."WCR"\Forearm.seek 3 + 300 counts M&d'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +70 h '&0 ."MFBPCR"delay 3 seconds +80 h / ."downElevator"\SE.seek :down M&;& A('X h & M&]% h %B ."WCR"\Forearm.seek :retract M&r'\ +91 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +35 ."MFBPCR"\ATV.dial :waste2 F*p % +13 h (ӷ M&L& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +29 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +62 h ' ."WCR"\CC.seek :closed C'V(W +416 h (W ."WCR"Hand.open M&>1 +82 h 'R ."MFBPCR"\ATV.dial :air2 F*K  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *B' +46 h 1 M&@% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)j+ +7 h + S)Q% h % C'7& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'/  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +22 h F*@% h % ."MFBPCR"\AS.seek 0.23ml F*Q' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h % ."MFBPCR"\AS.seek 0.255ml F*k'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*=% h %X ."MFBPCR"\AS.seek 0.24ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*| n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *-% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *a'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*5 @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*[ I +86 h I ."MFBPCR"\AS.seek 0ml F*J'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*%'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*E% h %\  ."MFBPCR"\AS.seek 0.5ml F*^'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*s% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*O% h %\  ."MFBPCR"\AS.seek 0.5ml F*R'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*:% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *n' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1217h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*~  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*_'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*B n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *u'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*1  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*)  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*X' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*8  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*t% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*.  +65 h ."MFBPCR"\ARV.dial :air1 F*P  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*e% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226146 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'$ @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'C @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'\\  +94 h  C'"% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'T' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'S  '*  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'y'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'Z  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'q  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'`  +37 h  C'f% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'D' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '#' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'9 @ +75 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C'4 ` +57 h ` ."WCR"\CS.seek 7.288ml C'h' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'M  '}  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '+'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '<' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ';' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)z% h % ."WCR"\CBV.dial between CSV and waste2b C']  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'p ` +57 h ` ."WCR"\CS.seek 7.288ml C'L' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'd  'W  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'(  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ''' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'{  +150 h C'r& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&>% h %\  A(@% h %X A(K. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (7( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (/& h &  M&Q& h &ӷ M&g% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &=( +142 h ( M&k& h & A(|& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(j& h &  ."WCR"\SE.seek 11.71 Pucks below Top Plate A(-'Ɛ +145 h 'Ɛ M&I& h & A(a& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &[' +26 h '  ."WCR"\Elbow.hold M&b, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(J- h -+Ɛ ."WCR"\SE.seek 12.04 Pucks below Top Plate A(&'3 +27 h '3 M&% h %  A(%& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&E'8 +20 h '8 M&& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &^' +22 h ' ."WCR"\SE.seek 13.04 Pucks below Top Plate A(O'" +37 h '" M&& h & M&:% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +89 h '\  ."WCR"\Elbow.seek CC M& (ӷ +142 h (ӷ M&n& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&~' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(o& h &  A(_& h &  M&B& h &ӷ M&m% h % ."WCR"\Forearm.seek :retract M&H'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &!( +143 h ( M&& h & A(l& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &c' +92 h '  ."WCR"\Elbow.hold M&1, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&u% h %  A()& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&& h & A(F& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &3' +22 h ' A(X% h %" ."downElevator"\SE.seek :down A(8'X +100 (N% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +33 h 'X ."downElevator"\SE.coast A(5 +57 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M& 1 +2 h 1 M&Y& h & M&t% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.seek :garage M&P'η +90 h 'η .tHand.close M&e/ +150 h / M&?& h &- M&% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &C' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'\\& h &W .tHand.open M&"1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'*& h &W C'S% h % .t\CSV.dial CSR C'T  +37 h  C'Z% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '`'[ +463 h '[ .t\CSV.dial :waste C'f +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C'9% h %4 .t\CSV.dial :flush C',  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CTV C'}  .t\CTV.dial CSV C'M  +37 h  +113  .t\CBV.dial :waste1 C'h @ +76 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'+& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'z  +37 h  .t\CBV.dial :waste2b C']  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'p& h &W C';% h %4 .t\CSV.dial :flush C'W  +75 h  .t\CS.seek 5ml C'd'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'(% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ 'r  +38 h +75 .t\CTV.dial :air C'>  +150 h .t\CS.seek 0ml C'@'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C'/ +38 h .t\CS.seek 0ml C'Q'4 +506 h '4 C'g& h &W C'k% h %4 .t\CSV.dial :flush C'|  +38 h  .t\CS.seek 3ml C'j'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'I% h %0 .t\CS.seek 5ml C'a'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'J'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'% +75 h .t\CS.seek 0ml C'\ '4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C's% h %4 .t\CSV.dial CSR C'  +150 h  C':% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'=' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'- +113 h .t\CTV.dial :intake C'[  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'n' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'E'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'&  +112 h  C'^% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'R  .t\CTV.dial CSV C'V  +37 h  +113  .t\CBV.dial :waste1 C'o @ +38 h @ .t\CS.seek 0ml C'_'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'B  +37 h  C'm% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'~  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'u  +38 h  C')% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +709 h ' ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'H  +38 h +75 .t\CTV.dial :air C'!  +151 h .t\CS.seek 0ml C'N'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C' '4 +506 h '4 #t\Flushing upper manifold C'Y& h &W C't% h %4 .t\CSV.dial :flush C'c  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +38 h @ C'% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'8'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'1 +38 h .t\CS.seek 0ml C'e'4 +506 h '4 C'?& h &W C'% h %4 .t\CSV.dial :flush C'U  +38 h  .t\CS.seek 3ml C'P'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'2'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'*% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.seek 5ml C'T'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +38 h @ C'C% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'q  'y  +37 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C'`'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'D& h &W C'9% h %4 .t\CSV.dial :flush C',  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'}  +38 h  .t\CBV.dial :waste2b C'M  +75 h  .t\CS.seek 0ml C'h'4 +766 h '4 #tDisinfecting external Intake with kill C'#& h &W C'+% h %4 .t\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'<' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'z  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C']' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +607 h '4 .t\CSV.dial CSR C';  +113 h  C'W% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'L' +1372 h ' .t\CSV.dial CBV C'( +113 h .t\CS.seek 0ml C'd'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&r& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +6 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&@' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'K( +416 h ( M&g& h &ӷ M&k% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Q(- +265 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &j'η +91 h 'η .tHand.open M&a1 +150 h 1 M&J% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&s& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&-/ +150 h / M&[& h &- M&=% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &E' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'R& h &W .tHand.open M&V1 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'B& h &W C'm% h %4 .t\CSV.dial CSR C'_  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'~'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'l'[ +463 h '[ .t\CSV.dial :waste C'u +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ')'4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'H  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'X' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! +76 h .t\CTV.dial :intake C'F  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'N'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'Y% h %4 .t\CSV.dial :flush C't  +75 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'O% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'3'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'.'0 +506 h '0 .t\CSV.dial :waste C'8 +38 h .t\CS.seek 0ml C'1'4 +506 h '4 C'7& h &W C'?% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'U'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +37 h @ C'$% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'P'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'2 +75 h .t\CS.seek 0ml C'\\'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C'C( +415 h ( M&T& h &ӷ M&q% h % .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &f(- +265 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &9'η +91 h 'η .tHand.open M&,1 +150 h 1 M&`% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST13-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:14:00CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST13-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1217h1500ml.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*}  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*z% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +64 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*/ % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*|  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1219h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*^% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*K n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*o  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*B  +21 h ."MFBPCR"delay 2 seconds +200 F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*l  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*!'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*N  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*O% h % \\ ."MFBPCR"\AS.seek 0.326ml F*c'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1219h1500ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*.  +22 h ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*$'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*P'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F**  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *n'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*9% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*}'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*w% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*+  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1221h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ARV.dial :carrier F*W  +11 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*/  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*g n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*x% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*6 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.53ml F*{' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*a % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*|'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*s n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*&'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1221h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +21 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*d% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*H% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*N % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*c'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*?'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1539381618.04 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1300h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*n% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *''Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*y I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*`' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*4 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*i' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*M  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.536ml F*}'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*+  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*# n +21 h n ."MFBPCR"delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*<'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1300h1500ml.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*L  +21 h ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b */'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*k% h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*a  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*{% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*\ % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*^'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*&'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1302h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*H  +43 h  ."MFBPCR"\ARV.dial :carrier F*l  +10 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*N  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*t I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*5 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*.  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.536ml F*c'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*8  +22 h  ."MFBPCR"delay 7 seconds +700 F*?% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*e n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*2' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*P  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Z'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*n % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1302h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*S @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*b  +150 h F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*,  +43 h  F*"% h % ."MFBPCR"\AS.seek 0.62ml F*y'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*9 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *4'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*G'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*` % +43 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *w' +423 h ' ."MFBPCR"\ATV.dial :air3 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.71ml F*#' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*]% h % zG ."MFBPCR"\AS.seek 0.365ml F*z' +7161 h ' ."MFBPCR"\ATV.dial :waste F*W n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*; % +43 h % F*<% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*r% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %  ."MFBPCR"\AS.seek 0.604ml F*/'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*x% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*6 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*k  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*{ % +108 h % F*@% h %V ."MFBPCR"\AS.seek 0.01ml F*J' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*|% h % ."MFBPCR"\AS.seek 0.58ml F*s' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*-'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*=% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*j' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*[  +21 h  ."MFBPCR"\AS.seek 0ml F*Q'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*^  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*R n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *&'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +202 h 'R ."MFBPCR"\SPE.coast F*m  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*V'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*B n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*E  +43 h  F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*~ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *l': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*)  +65 h  F*u% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*X n +65 h n F*F% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *!' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *N'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*Y  +65 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *3'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*O n +65 h n F*.% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *7' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*?  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *c': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*e n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*1 n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*$  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*U % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +21 h F*2% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*Z' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*q% h % ."MFBPCR"\AS.seek 0.97ml F*T'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *"'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *9'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *''R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*4  +43 h  F*`% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *G' +9988 h ' ."MFBPCR"\ATV.dial AIV F*i I +43 h I ."MFBPCR"\AS.seek 0.02ml F*w' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*}'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*+% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*# % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*<% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*r  +64 h  F*L% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*s'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *='R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *&'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*o % +108 h % ."MFBPCR"\AS.seek 0ml F*B'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990523 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )E h  C' '_ h  P% %V h  F* *~ h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ S)&\ P% *\ F*\ 9\ C' 2\ .t\SPE.coast P% 0\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *d% h %V .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'H& S)#.0X 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:15:00CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #13) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&Z1 +150 h 1 M&& h &M M&T% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &,(- +166 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &9'η +91 h 'η .tHand.close M&y/ +150 h / M&4& h &- M&`% h %η .t\Forearm.seek :retract M&''\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +264 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'+(W +416 h (W C'w& h &W .tHand.open M&}1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'h% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'W'[ +463 h '[ .t\CSV.dial :waste C'] +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ';'4 +2009 h '4 #t\Purging external Intake C'<& h &W C'z% h %4 .t\CSV.dial :flush C'(  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'L .t\CBV.dial CSV C'p  h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'>'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'g& h &W C'x% h %4 .t\CSV.dial :flush C'/  +75 h  .t\CS.seek 5ml C'k'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CBV C'{ .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'@'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'|'4 +507 h '4 C'I& h &W C':% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'J'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C's% h %0 .t\CS.seek 5ml C'='p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'-'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'[ +75 h .t\CS.seek 0ml C'j'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&Q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +7 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'm( +416 h ( M&K& h &ӷ M&o% h % .t\Forearm.seek :retract M&&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +264 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &v'η +91 h 'η .tHand.open M&B1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:15:05CEST30-Sep-18\\n{1 => 22, 2 => 14, 3 => 8, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:15:00CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F* % #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'S& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&E% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(_% h %X A(V. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (~( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*d % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (H& h & M& & h &- M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &u( +166 h ( M&l& h & A(X& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &F' +90 h ' A(& h & .t\SE.seek 6.71 Pucks below Top Plate A( ' +195 h ' A(!& h & M&N& h & .t\Elbow.seek 3 M&( +7 h ( M&t& h & A(5& h & .t\Forearm.seek 3 + 300 counts M&Y'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &O/ +150 h / ."downElevator"\SE.seek :down M&.& h & A( 'X M&3% h %B .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.seek CC M&1(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M&?& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +90 h ' .t\CC.seek :closed C'n(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)U+ +7 h + S)% h % C'q& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  .t\SS.seek 0ml S)' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)  +144 h  +6  S)k% h % .t\CBV.dial between SSV and waste2 C'{  +19 h  C'6% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'@  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +2609 h '4 C'a% h %4 S)I% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C': @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'x9 h 9Td +100 C'9 h 9kd +100 C'/9 h 9d +100 C'>9 h 9d +100 C'9 h 9d +100 C'k9 h 9d +100 C'{9 h 9d +100 C'69 h 9d +100 C'@9 h 9 d +100 C';9 h 9 d +100 C'g9 h 96d +100 C'a9 h 9Ld +100 C'I9 h 9bd +100 C':9 h 9xd +100 C'9 h 9d +100 C'|9 h 9d +100 C'\ 9 h 9d +100 C's9 h 9d +100 C'9 h 9d +100 C'J9 h 9d +100 C'-9 h 9d +100 C'[9 h 9(d +100 C'Q9 h 9>d +100 C'=9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'^9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'#9 h 9d +100 C'+9 h 9d +100 C'o9 h 9  d +100 C'f9 h 9 d +100 C'M9 h 9 *d +100 C'R9 h 9 ?d +100 C'\\9 h 9 Td +100 C'B9 h 9 j d +100 C'K9 h 9 $d +100 C'%9 h 9 (d +100 C'&9 h 9 ,d +100 C' 9 h 9 0d +100 C'E9 h 9 4d +100 C'_9 h 9 8d +100 C'V9 h 9 & h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &k' +22 h ' .t\SE.seek 14.04 Pucks below Top Plate A({' +36 h ' M&6& h & M&@% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +90 h '\  .t\Elbow.seek CC M&g(ӷ +142 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.seek CC M&:' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'|( +416 h ( A(\ & h &  A(s& h &  M&& h &ӷ M&J% h % .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &[( +142 h ( M&Q& h & A(=& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &^1 +150 h 1 M&% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&#'8 +21 h '8 M&+& h & A(o& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &f' +22 h ' A(M% h % ."downElevator"\SE.seek :down A(R'X +100 (\\% h %v M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'% @ +19 h @ .t\CS.seek 10ml C'&' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C'E { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'_& h & .tHand.open M&V1 +2 h 1 M&& h & M&d% h %\  .t\Elbow.seek FlushPuck::Garage M&0(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &- .t\Forearm.seek :garage M&v'η +91 h 'η .tHand.close M&S/ +150 h / M&l& h &- M&X% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +265 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +90 h ' .t\CC.seek :closed C'C(W +416 h (W C'!& h &W .tHand.open M&O1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%5 +151 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%u' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%F  +38 h  .t\PS.seek 0ml P%3' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%?' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%Y  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%7  h .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%m' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%~' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%U' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&e& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +7 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C'( +416 h ( M&`& h &ӷ M&2% h % .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +264 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &i'η +91 h 'η .tHand.open M&b1 +150 h 1 M&T% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C' & h & M&% h %\  A(h% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (w( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &]( +166 h ( M&W& h & A(z& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &1' +90 h ' A(& h & .t\SE.seek 5.71 Pucks below Top Plate A(p' +205 h ' A(r& h & M&x& h & .t\Elbow.seek 3 M&( +6 h ( M&c& h & A(>& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &6/ +150 h / ."downElevator"\SE.seek :down A(@'X M&<& h & M&% h %B .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.seek CC M&L(ӷ +114 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' .t\CC.seek :closed C'j(W +416 h (W .tHand.open M&k1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C';  +150 h  .t\CSV.dial CTV C'n  +19 h  .t\CS.seek 5ml C'\ 'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C's' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%J  +38 h  .t\PS.seek 2.5ml P%Q' +2610 h ' .t\PSV.dial :mixloop P%= +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%:  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%g +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%^  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'+  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'o @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'M  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'- ` +18 h ` .t\CSV.dial :air C'[ @ +38 h @ .t\CS.seek 10ml C'\\' +2647 h ' .t\CSV.dial CTV C'B  +38 h  .t\CS.seek 0ml C'%'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'E  +151 h C'_& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&V% h %\  A(% h %X A(d. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (#& h &  M&{& h &ӷ M&f% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +142 h ( M&)& h & A(R& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +90 h ' A(9& h &  .t\SE.seek 13.71 Pucks below Top Plate A('n +125 h 'n M&S& h & A(l& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &X' +25 h '  .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(v- h -+n M&$&.,(\ #b .t\SE.seek 14.04 Pucks below Top Plate A(A' +27 h ' M&N% h %  A(0& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&|& h & A(!& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &O' +22 h ' .t\SE.seek 15.04 Pucks below Top Plate A(H' +36 h ' M&5& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +90 h '\  .t\Elbow.seek CC M&F(ӷ +142 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.seek CC M&3' +91 h ' .tHand.close M&Y/ +150 h / .t\CC.seek :open C' ( +416 h ( A(& h &  A(7& h &  M&?& h &ӷ M&% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&m& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &q' +92 h '  .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &~1 +150 h 1 M&e% h %  A("& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&D'8 +21 h '8 M&Z& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(C% h % ."downElevator"\SE.seek :down A(`'X +100 (2% h %- M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +13 h 'X ."downElevator"\SE.coast A(5 +76 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%,  +113 h  P%P% h % .t\PS.seek 0.499ml P%b' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%T +38 h .t\PRV.dial :air P%y  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%h  +18 h  .t\PRV.dial :air P%  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %i' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&z1 +2 h 1 M&& h & M&4% h %\  .t\Elbow.seek FlushPuck::Garage M&r(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&>/ +150 h / M&6& h &- M&<% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'p& h &W .tHand.open M&1 +150 h 1 M&@% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%k @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%;  +76 h  .tdelay 2 seconds +200 P%n% h % .t\PS.seek 1.499ml P%/' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%w' +1094 h ' .t\PSV.dial :air P%&  +37 h  .t\PS.seek 0ml P%\ ' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%s +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%J  +37 h  .tdelay 2 seconds +200 P%U% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %='r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%:  +75 h .tdelay 2 seconds +200 P%g% h %r .t\PS.seek 0.475ml P%^' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%Q  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%+  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%o  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %M' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%- +75 h .t\PTV.dial :PRV,avoiding: :puck P%[ @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%B  +75 h  .t\PS.seek 2.5ml P%\\' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%E' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%_  +75 h  .t\PS.seek 2.5ml P%V' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%d  +75 h P%#% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %{' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'f & +50 h & .t\AWV.select :waste C' +6 P%)% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %R'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'9 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %L' +1312 h ' .t\PSV.dial :air P%S  +38 h  .t\PS.seek 2.5ml P%l' +2610 h ' .t\PSV.dial :PML P%v +37 h .t\PS.seek 0ml P%N' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '0& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'|& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*! @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%5 +22 h  ."MFBPCR"delay 20 seconds +91 h P%.% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%}' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *X'6 +66 .t\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%*' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%A' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%O +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*Y  +22 h ' .t\PSV.dial PSR P%t +21 h  F*j% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%7% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%?' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%3' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*F  +21 h  F*~% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *e' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%" +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Z' +217 ."MFBPCR"\ATV.dial :AIV F*$ I +43 h I F*C% h % ."MFBPCR"\AS.seek 0.469ml F* ' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%2' +2610 h ' .t\PSV.dial PSR P% +113 h P%q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%H  +38 h  .t\PS.seek 0ml P%,' +739 h ' ."MFBPCR"delay 5 seconds +500 F*P9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*`% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%T' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%y +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P% +113 h P%h% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%b' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%4  +150 h  .t\PS.reconfigure PSconfig P%#A d`xb .t\PS.seek 0ml P%x' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*' I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%>  +113 h  .t\PS.seek 2.5ml P%6' +2610 h ' .t\PSV.dial :mixloop P%< +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %G' +5106 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %1' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*a  +43 h  F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*p% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +170 h ' .t\PSV.dial :mixloop P%@ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F*k n +129 h n ."MFBPCR"\AS.seek :empty F*I'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*;% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *n' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*i  +43 h F*/% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *&'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'w  +37 h  C's% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'U' +1625 h ' ."connect"\CSV.dial CTV C'\  .t\CTV.dial CSV C':  +38 h  +113  .t\CBV.dial :waste1 C'g @ +38 h @ .t\CS.seek 0ml C'='4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*Q  +22 h ."MFBPCR"delay 3 seconds +300 F*^% h %_ ."MFBPCR"\AS.seek 0.434ml F*+'ݏ +110 h '4 .t\CSV.dial CSR C'o  +37 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'-'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*[  +22 h F*M% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*B'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +107 h n F* % h %& ."MFBPCR"\AS.seek 0.092ml F*\\'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'_' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'd& h &W #tGaraging FlushPuck in Collection Clamp M&#& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +7 h (ӷ M&f& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C'R( +416 h ( M&S& h &ӷ M&L% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &l(- +264 h (- M&0& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&51 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C' & h & M&% h %\  A(u% h %X A(8. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (}( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&*& h &- M&O% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &A( +166 h ( M&Y& h & A(j& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &t' +89 h ' A(7& h & ."WCR"\SE.seek 4.71 Pucks below Top Plate A(X' +215 h ' A(K& h & M&& h & ."WCR"\Elbow.seek 3 M&( +6 h ( M&& h & A(F& h & ."WCR"\Forearm.seek 3 + 300 counts M&~'B +23 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &?/ +40 h '&0 ."MFBPCR"delay 3 seconds +110 h / ."downElevator"\SE.seek :down M&"& A(m'X h & M&$% h %B ."WCR"\Forearm.seek :retract M&C'\ +90 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +100 ."MFBPCR"\ATV.dial :waste2 F*e % +24 h 'X ."downElevator"\SE.coast A(5 +18 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R + h (ӷ M&Z& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' ."WCR"\CC.seek :closed C'2(W +416 h (W ."WCR"Hand.open M&H1 +52 h 'R ."MFBPCR"\ATV.dial :air2 F*D  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *P' +76 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +6 h + S)% h % C',& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'y  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*6'\  +1032 h '\  F*c% h %\  ."MFBPCR"\AS.seek 0.5ml F*G'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*a% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *x' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*< % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1317h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*p  +64 h  ."MFBPCR"\ARV.dial :carrier F*@  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*i  +22 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*n  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*s% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*%' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*g % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.536ml F*:'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +107 h  ."MFBPCR"delay 7 seconds +700 F*o% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Q n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*&'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*+  +43 h  ."MFBPCR"\ASV.dial :waste1 F*B  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*E% h % \\ ."MFBPCR"\AS.seek 0.326ml F*_'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226175 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'f  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'd @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'] @ +75 h @ +50 S))% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'S% h % ."WCR"\CSV.dial between air and CTV C'( ` +19 h ` ."WCR"\CS.seek 7.288ml C'{' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'L  '0  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'V'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'5  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '|' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'v  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'.  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'8' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)u% h % ."WCR"\CBV.dial between CSV and waste2b C'O  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'* ` +56 h ` ."WCR"\CS.seek 7.288ml C'j' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'7  'Y  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'N'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'K  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'l' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'F  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'?' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'A @ +75 h @ +50 S)t% h % ."WCR"\CBV.dial between CSV and waste2b C'$  +94 h  C'Z% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'e' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'X  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'm'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'~  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'C' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'R  +150 h C'\\& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&H% h %\  A(% h %X A(D. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (,( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &ӷ M&q% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &}( +143 h ( M&y& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &h' +89 h ' A( & h &  ."WCR"\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] M& & h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &' +26 h '  ."WCR"\Elbow.hold M&b, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(z- h -+] ."WCR"\SE.seek 15.04 Pucks below Top Plate A(4' +27 h ' M&% h %  A(& h &  ."WCR"\Forearm.force -20 .\Forearm.seek 2 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &W'8 +20 h '8 M&'& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &>' +22 h ' ."WCR"\SE.seek 16.04 Pucks below Top Plate A(c' +37 h ' M&6& h & M&a% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +89 h '\  ."WCR"\Elbow.seek CC M&<(ӷ +142 h (ӷ M&x& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ ."WCR"\Forearm.seek CC M&p' +91 h ' ."WCR"Hand.close M&@/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(& h &  A(r& h &  M&P& h &ӷ M&k% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&i& h & A(/& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &;' +92 h '  ."WCR"\Elbow.hold M&n, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &s1 +150 h 1 M&I% h %  A(J& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&\ '8 +21 h '8 M&w& h & A(g& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &U' +22 h ' A(%% h % ."downElevator"\SE.seek :down A(^'X +100 (o% h %X M&:% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'=& h & .tHand.open M&1 +2 h 1 M&M& h & M&[% h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.seek :garage M& 'η +90 h 'η .tHand.close M&E/ +150 h / M&& h &- M&-% h %η .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &f(ӷ +265 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &]' +91 h ' .t\CC.seek :closed C')(W +416 h (W C'& h &W .tHand.open M&S1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'0& h &W C'L% h % .t\CSV.dial CSR C'{  +37 h  C'5% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'V'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '.'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '|'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'8& h &W C' % h %4 .t\CSV.dial :flush C'u  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'O'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'*% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Y  '7  +37 h  +113  .t\CBV.dial :waste1 C'j @ +76 h @ .t\CS.seek 0ml C'K'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'N& h &W C'% h %4 .t\CSV.dial :flush C'F  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'?% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CTV C't  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'$  +76 h  .t\CS.seek 0ml C'Z'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'"% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'X'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +38 h @ C'~% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R '\\  +38 h +75 .t\CTV.dial :air C'H  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'q& h &W C'y% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C' '0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +38 h @ C' % h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'`% h %4 .t\CSV.dial CSR C'6  +150 h  C'a% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'h'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'}' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'4  +112 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  '2  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'r'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'P  +37 h  C'k% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'i'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'p  +37 h  .t\CBV.dial :waste2b C'<  +76 h  .t\CS.seek 0ml C's'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'I  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '%  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'o'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +506 h '0 .t\CSV.dial :waste C'= +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'M& h &W C'[% h %4 .t\CSV.dial :flush C';  +38 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +38 h @ C'+% h %p .t\CS.seek 7ml C'B'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +506 h '0 .t\CSV.dial :waste C'n +38 h .t\CS.seek 0ml C'E'4 +506 h '4 C'& h &W C'-% h %4 .t\CSV.dial :flush C'Q  +38 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +37 h @ C'% h %0 .t\CS.seek 5ml C'&'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'#'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'S'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'(& h &W C'0% h %4 .t\CSV.dial :flush C'L  +38 h  .t\CS.seek 5ml C'{'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C']% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  'V  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'.'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'8& h &W C' % h %4 .t\CSV.dial :flush C'u  +75 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'*% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Y  +38 h  .t\CBV.dial :waste2b C'7  +75 h  .t\CS.seek 0ml C'j'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'N% h %4 .t\CSV.dial CSR C'  +37 h  C'F% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'K'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'? '  +38 h +75 .t\CTV.dial :intake C't  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'$' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'A  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'"  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'Z'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'e' +1372 h ' .t\CSV.dial CBV C'~ +113 h .t\CS.seek 0ml C'X'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&\\& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +6 h (ӷ M&H& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'D( +416 h ( M&q& h &ӷ M&y% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&3& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&`& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &6(- +6 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &- .t\Forearm.seek :garage M&h'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&}% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +264 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &W' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'G& h &W .tHand.open M&21 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'P& h &W C'k% h %4 .t\CSV.dial CSR C'r  +113 h  C'i% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'p'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '<% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '/'[ +463 h '[ .t\CSV.dial :waste C'I +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +2010 h '4 #t\Purging external Intake C's& h &W C'w% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +76 h .t\CTV.dial :intake C'g  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'o'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'=  +76 h #t\Flushing upper manifold C':& h &W C'M% h %4 .t\CSV.dial :flush C'[  +75 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'c% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\ +113 h .t\CTV.dial :air .\CS.seek 0ml C'U'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'B'0 +506 h '0 .t\CSV.dial :waste C'^ +38 h .t\CS.seek 0ml C'n'4 +506 h '4 C',& h &W C'% h %4 .t\CSV.dial :flush C'-  +38 h  .t\CS.seek 3ml C'Q'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +37 h @ C'd% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +506 h '0 .t\CSV.dial :waste C'# +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +7 h (ӷ M&0& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C']( +415 h ( M&{& h &ӷ M&v% h % .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&u1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST14-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:16:48CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST14-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1317h1500ml.pcr F**% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Y  +22 h ."MFBPCR"\ATV.dial :air3 F*7 % +86 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*A  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +64 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +21 h F*q% h % ."MFBPCR"\AS.seek 0.81ml F*y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1319h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ARV.dial :carrier F*4  +11 h  F*>% h %V ."MFBPCR"\AS.seek 0.5ml F*6'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*P  +21 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*i  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*W% h %Fj ."MFBPCR"\AS.seek 0.524ml F*X' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*<  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.53ml F*I' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*/  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*g n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*='\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*:  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*;'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*+ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1319h1500ml.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*B  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*E% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +21 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*0  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*L  +65 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *x'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*8  +31 h  ."MFBPCR"\ATV.dial :air3 F*( % +87 h % F*u% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +22 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*|'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1321h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*Z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\\'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*q n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*3 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.53ml F*R' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*z  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*` n +21 h n ."MFBPCR"delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*h% h % \\ ."MFBPCR"\AS.seek 0.326ml F*4'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1321h1500ml.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +21 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*s  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*%% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +22 h F*\ % h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*;'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1539468018.04 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1400h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*@'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*8  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*(  +22 h ."MFBPCR"delay 2 seconds +200 F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *C'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*V I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F** n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*!' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*7  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.536ml F*Y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +108 h  ."MFBPCR"delay 7 seconds +700 F*|% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*K'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*A  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*$% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1400h1500ml.pcr F*"% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*e  +21 h ."MFBPCR"\ATV.dial :air3 F*~ % +87 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*m'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*R% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *z'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*>'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*4'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *k'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1402h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*/  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*=  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *M'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*[ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*T % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*+' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*;'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*n'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +21 h n ."MFBPCR"delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.6ml F*Q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*0% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1402h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*L @@T *@  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*b  +150 h F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *8' +424 h ' ."MFBPCR"\ATV.dial :ASV F*( n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*u  +43 h  F*S% h % ."MFBPCR"\AS.seek 0.62ml F*V'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b **'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*_'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *O' +423 h ' ."MFBPCR"\ATV.dial :air3 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*Y% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*F' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*K% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*A n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*$% h % zG ."MFBPCR"\AS.seek 0.365ml F*t' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*" % +43 h % F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*\\% h % ."MFBPCR"\AS.seek 0.579ml F*Z' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*H% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*9% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*m' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*3 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*y  +22 h  ."MFBPCR"\AS.seek 0ml F*)'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*R % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*`' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*'  +22 h F*}% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*h n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *6'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*>  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b * ' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*G n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *4'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*D n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*k  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*2'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*P n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*W  +43 h  F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *i' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*p I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*s  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b */': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*J  +65 h  F*I% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *w'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*g% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*= I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*:  +43 h  F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*M  +65 h  F*T% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *U'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*c n +65 h n F*B% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *,' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *;': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*E n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *-'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*n n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*d  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*Q % +43 h % F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*#% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %0 ."MFBPCR"\AS.seek 0.985ml F*5' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*v% h % ."MFBPCR"\AS.seek 0.97ml F*{'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*( I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *S'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *C'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F**  +43 h  F*.% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *_' +9988 h ' ."MFBPCR"\ATV.dial AIV F*! I +43 h I ."MFBPCR"\AS.seek 0.02ml F*O' +9880 h ' ."MFBPCR"\ATV.dial :waste F*| n +129 h n ."MFBPCR"\AS.seek 0ml F*Y'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*N% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *K'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*$'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*t'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*l% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*~  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +64 h  F*e% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *f'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +32 h  ."MFBPCR"\ATV.dial :air3 F*m % +86 h % F*)% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*k n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *4'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*P'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990523 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )W h  C' 'r h  P% %2 h  F* *p h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *i  h  .t\SPE.configure SPEconfig F*\ 2 *X% h %V .t\AS.configure ASconfig S)s% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'%& S)#.0X 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:17:47CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #14) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'v& h & .tHand.open M&51 +150 h 1 M&(& h &M M&{% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &u(- +166 h (- M&S& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&V/ +150 h / M&*& h &- M&.% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &7(ӷ +264 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &|' +91 h ' .t\CC.seek :closed C'N(W +416 h (W C'O& h &W .tHand.open M&Y1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'?& h &W C'j% h %4 .t\CSV.dial CSR C'F  +150 h  C'A% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'K'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'$ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '"'4 +2009 h '4 #t\Purging external Intake C'l& h &W C't% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'e '  h +75 .t\CTV.dial :intake C'f  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'H'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Z  +75 h #t\Flushing upper manifold C'q& h &W C'9% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'm @ +37 h @ C')% h %p .t\CS.seek 7ml C'3'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C' & h &W C'a% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +38 h @ C'`% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'h'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&G' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'k( +416 h ( M&D& h &ӷ M&% h % .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &]'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:17:53CEST30-Sep-18\\n{1 => 22, 2 => 17, 3 => 5, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:17:47CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'L& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&W% h %\  A(r% h %X A(2. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (p( +6 F*i% h %&K ."readyMFB"\ATV.dial :waste2 F*X % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *s' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (%& h & M&<& h &- M&J% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &I( +166 h ( M&/& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &g' +90 h ' A(w& h & .t\SE.seek 3.71 Pucks below Top Plate A(=' +223 h ' A(& h & M&o& h & .t\Elbow.seek 3 M&:( +7 h ( M&[& h & A(T& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down M&M& A(B'X h & M&U% h %B .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.seek CC M&n(ӷ +133 h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'x(W +416 h (W .tHand.open M&@1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +90 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)Q+ +7 h + S)% h % C'v& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'4 +7 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S)6 +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'99 h 9Td +100 C'f9 h 9kd +100 C'9 h 9d +100 C'H9 h 9d +100 C')9 h 9d +100 C'y9 h 9d +100 C'R9 h 9d +100 C'39 h 9d +100 C'9 h 9 d +100 C'"9 h 9 d +100 C'q9 h 96d +100 C'9 h 9Ld +100 C' 9 h 9bd +100 C'a9 h 9xd +100 C'm9 h 9d +100 C'9 h 9d +100 C''9 h 9d +100 C'`9 h 9d +100 C'9 h 9d +100 C'z9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'}9 h 9Td +100 C'69 h 9id +100 C'9 h 9d +100 C'>9 h 9d +100 C'h9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'N9 h 9d +100 C'9 h 9  d +100 C'P9 h 9 d +100 C'79 h 9 *d +100 C'G9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C'D9 h 9 $d +100 C'9 h 9 (d +100 C'49 h 9 ,d +100 C'9 h 9 0d +100 C'W9 h 9 4d +100 C'r9 h 9 8d +100 C'29 h 9 1 +150 h 1 M&h% h %  A( & h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&N& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &P' +22 h ' A(7% h % ."downElevator"\SE.seek :down A(G'X +93 h 'X ."downElevator"\SE.coast A(5 +7 (% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +18 h @ .t\CS.seek 10ml C'4' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'W { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'r& h & .tHand.open M&21 +2 h 1 M&i& h & M&X% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.seek :garage M&]'η +91 h 'η .tHand.close M&L/ +150 h / M&/& h &- M&% h %η .t\Forearm.seek :retract M&w'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'1& h &W .tHand.open M&c1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%[ +150 h P%M% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%I' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%g  +38 h  .t\PS.seek 0ml P%U' +1597 h ' .t\PSV.dial PSR P%0 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'=  h +76 .t\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%,  h .t\PS.seek 0ml P%B' +2609 h ' .t\PSV.dial :air P%@  +38 h  .t\PS.seek 2.5ml P%k' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%v' +2610 h ' .t\PSV.dial :air P%\  +38 h  .t\PS.seek 2.5ml P%p' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .t\PS.seek 0ml P%Q' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&E& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C'+( +416 h ( M&.& h &ӷ M&#% h % .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &*(- +264 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +91 h 'η .tHand.open M&b1 +150 h 1 M&(% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'& h & M&?% h %\  A(j% h %X A(F. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (O( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (|& h & M&&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &$( +166 h ( M&& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &n' +89 h ' A(A& h & .t\SE.seek 2.71 Pucks below Top Plate A(') +235 h ') A(\\& h & M&9& h & .t\Elbow.seek 3 M&f( +6 h ( M&H& h & A()& h & .t\Forearm.seek 3 + 300 counts M&;'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &3/ +150 h / ."downElevator"\SE.seek :down M&l& A('X h & M&K% h %B .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' + h 'X ."downElevator"\SE.coast A(5 +90 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&y1 +150 h 1 M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'"  +150 h  .t\CSV.dial CTV C'x  +19 h  .t\CS.seek 5ml C'''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'`' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%z  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%} +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%a  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%q +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%>  +150 h  .t\PS.seek :empty P%h' +2610 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'N  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'7  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'W  +151 h C'r& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&2% h %\  A(i% h %X A(X. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (6( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&R& h &ӷ M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &P( +142 h ( M&<& h & A(J& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Y' +90 h ' A( & h &  .t\SE.seek 16.71 Pucks below Top Plate A('; +95 h '; M&L& h & A(/& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +26 h '  .t\Elbow.hold M&D, +6 h , .t\Elbow.reconfigure TightElbow A(]- h -+; M&$&.,(\ #b .t\SE.seek 17.04 Pucks below Top Plate A(' +27 h ' M&% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&w'8 +20 h '8 M&& h & A(1& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +23 h ' .t\SE.seek 18.04 Pucks below Top Plate A(%' +36 h ' M&[& h & M&M% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +89 h '\  .t\Elbow.seek CC M&g(ӷ +143 h (ӷ M&I& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ .t\Forearm.seek CC M&U' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'=( +416 h ( A(:& h &  A(,& h &  M&& h &ӷ M&@% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&k& h & A(\ & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &v' +93 h '  .t\Elbow.hold M&{, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &p1 +150 h 1 M&E% h %  A(S& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&8'8 +20 h '8 M&5& h & A(d& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &-' +22 h ' A(T% h % ."downElevator"\SE.seek :down A(.'X +84 h 'X ."downElevator"\SE.coast A(5 +16 (#% h %X M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +90 h '\  .t\SC.coast .\PSV.dial :air A(6 P%u  +113 h  P% % h % .t\PS.seek 0.499ml P%b' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%( +38 h .t\PRV.dial :air P%V  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'?  +38 h .t\PRV.dial between CBV and air P%j  +19 h  .t\PRV.dial :air P%F  +19 h  P%|% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %&' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %!' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&t1 +2 h 1 M&A& h & M&*% h %\  .t\Elbow.seek FlushPuck::Garage M&C(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&H'η +91 h 'η .tHand.close M&)/ +150 h / M&3& h &- M&l% h %η .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +264 h (ӷ M&n& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C';(W +416 h (W C'& h &W .tHand.open M&f1 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'+& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%y @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%Z  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%"  +75 h  .tdelay 2 seconds +200 P%x% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%O' +1094 h ' .t\PSV.dial :air P%4  +38 h  .t\PS.seek 0ml P%'' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%` +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%z  +38 h  .tdelay 2 seconds +200 P%Q% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %}'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%a  +75 h .tdelay 2 seconds +200 P%q% h %r .t\PS.seek 0.475ml P%>' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P% 'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%N  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %7' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%W' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%r  +76 h  .t\PS.seek 2.5ml P%2' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%i +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%X  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %R' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'G & +50 h & .t\AWV.select :waste C' +7 P%<% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %J'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %e' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :PML P%] +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*1 @@T *m  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%[ +22 h  ."MFBPCR"delay 20 seconds +91 h P%M% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*P I +128 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P%0' +1597 h ' .t\PSV.dial PSR P%w +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%c +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%D' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%o +21 h  F* % h %6 ."MFBPCR"\AS.seek 0.329ml F*:'ͯ +92 h P%,% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*@ % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\  +38 h  .t\PS.seek 0ml P%U' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*g  +22 h  F*p% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *E' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%S +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%5' +217 ."MFBPCR"\ATV.dial :AIV F*d I +43 h I F*T% h % ."MFBPCR"\AS.seek 0.469ml F*B' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%#' +2610 h ' .t\PSV.dial PSR P%s +113 h P%v% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%8' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +37 h  .t\PS.seek 0ml P%u' +740 h ' ."MFBPCR"delay 5 seconds +500 F* 9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*. n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*-% h % ."MFBPCR"\AS.seek 0.041ml F*6'% +336 h ' .t\PSV.dial PSR P%h +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%(' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%V +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%? +113 h P%j% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%F' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%|  +38 h  .t\PS.seek 0ml P%b' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%&' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %A'k +2678 h 'k .t\PSV.dial :waste1 P%*  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %\\' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*9 I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *{'V +850 h ' .t\PSV.dial :air P%)  +113 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%l +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %_' +5107 h ' .t\PSV.dial :air P%H  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %n' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *K'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*= I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*f' +171 h ' .t\PSV.dial :mixloop P%~ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %+' +618 h ' ."MFBPCR"\ATV.dial :waste F*y n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*Z  +85 h  F*"% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *x' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*!  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *4'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'O  +38 h  C'`% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'z'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'Q' +1626 h ' ."connect"\CSV.dial CTV C''  .t\CTV.dial CSV C'a  +37 h  +113  .t\CBV.dial :waste1 C'q @ +38 h @ .t\CS.seek 0ml C'}'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*>% h %_ ."MFBPCR"\AS.seek 0.434ml F*N'ݏ +110 h '4 .t\CSV.dial CSR C'  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F*7% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'i'4 +2648 h '4 C'X& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +6 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'J( +416 h ( M&L& h &ӷ M&e% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &m'η +91 h 'η .tHand.open M&[1 +150 h 1 M&M% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'P& h & M&% h %\  A(I% h %X A(^. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Y( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (w& h & M&0& h &- M&c% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +165 h ( M&& h & A( & h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &o' +90 h ' A(,& h & ."WCR"\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': A(D& h & M&@& h & ."WCR"\Elbow.seek 3 M&:( +6 h ( M&\ & h & A(g& h & ."WCR"\Forearm.seek 3 + 300 counts M&p'B +23 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +10 h '&0 ."MFBPCR"delay 3 seconds +140 h / ."downElevator"\SE.seek :down M&S& A(k'X h & M&d% h %B ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.seek CC M&U(ӷ +69 ."MFBPCR"\ATV.dial :waste2 F*E % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +30 h (ӷ M&5& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' ."WCR"\CC.seek :closed C'#(W +416 h (W ."WCR"Hand.open M&%1 +23 h 'R ."MFBPCR"\ATV.dial :air2 F*8  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b * ' +106 h 1 M&.% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)B+ +6 h + S)h% h % C'u& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'V  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +173 h n ."MFBPCR"\ASV.dial :SPEelution F*  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 * % h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *4' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *}% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*7% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *N' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F*'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*W % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *X'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F*G% h %v ."MFBPCR"\AS.seek 0.059ml F*<'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*$% h %H ."MFBPCR"\AS.seek 0.069ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*L% h % ."MFBPCR"\AS.seek 0.769ml F*e' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*[% h % k ."MFBPCR"\AS.seek 0.779ml F*M'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*] I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *P' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*I'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*^ % +43 h % F*w% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*m% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*,% h %  ."MFBPCR"\AS.seek 0.604ml F*/'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*D% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*@' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*g  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *d'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*o% h %V ."MFBPCR"\AS.seek 0.59ml F*E' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*5 n +107 h n ."MFBPCR"\ASV.dial :wasteR F*s% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*p  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*: I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*T' +625 h ' ."MFBPCR"\ATV.dial :air3 F*k % +21 h % F*J% h % ."MFBPCR"\AS.seek 0.13ml F*8' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*.% h % ."MFBPCR"\AS.seek 0.23ml F*h' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*V'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +21 h F*Y% h %X ."MFBPCR"\AS.seek 0.24ml F*B' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*j n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*?  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *F'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*( @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*6 I +86 h I ."MFBPCR"\AS.seek 0ml F*t'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F**  +43 h  #"MFBPCR"\Swish #1 of 2 F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *9'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*)'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*&% h %(A ."MFBPCR"\AS.seek 0.6ml F*3'\  +1031 h '\  F*H% h %\  ."MFBPCR"\AS.seek 0.5ml F*_'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *\\' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*l % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +3472 h 'V ."MFBPCR"\SPE.coast F*=  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1417h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*~  +11 h  F*K% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*!  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*x  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*`% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*z  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*q % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*N  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226206 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'G  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'X @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'$ @ +75 h @ +50 S)<% h % ."WCR"\CBV.dial between CSV and waste2b C';  +95 h  C'L% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'R' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'e  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '2'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'[  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C']  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'M  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'^' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'P @ +75 h @ +50 S)I% h % ."WCR"\CBV.dial between CSV and waste2b C'c  +94 h  C'm% h % ."WCR"\CSV.dial between air and CTV C'0 ` +56 h ` ."WCR"\CS.seek 7.288ml C' ' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C',  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'D  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '/' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'\  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'g  +38 h  C'@% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'd  +94 h  C'5% h % ."WCR"\CSV.dial between air and CTV C's ` +57 h ` ."WCR"\CS.seek 7.288ml C'E' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'p  ':  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'J  +150 h C'T& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&%% h %\  A(.% h %X A(8. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (u( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&h& h &ӷ M&v% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +142 h ( M&V& h & A(j& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &?' +89 h ' A(B& h &  ."WCR"\SE.seek 17.71 Pucks below Top Plate A(|'* +87 h '* M&& h & A(F& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &6' +25 h '  ."WCR"\Elbow.hold M&b, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(t- M&$&.,(\ #b h -+* ."WCR"\SE.seek 18.04 Pucks below Top Plate A(*' +26 h ' M&A% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +21 h '8 M&9& h & A(&& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &)' +22 h ' ."WCR"\SE.seek 19.04 Pucks below Top Plate A(H' +36 h ' M&3& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  ."WCR"\Elbow.seek CC M&l(ӷ +143 h (ӷ M&\\& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&~/ +150 h / ."WCR"\CC.seek :open C'K( +416 h ( A({& h &  A(C& h &  M& & h &ӷ M&y% h % ."WCR"\Forearm.seek :retract M&Z'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &f( +142 h ( M&!& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &"' +93 h '  ."WCR"\Elbow.hold M&x, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &`1 +150 h 1 M& % h %  A(z& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&''8 +20 h '8 M&O& h & A(q& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Q' +23 h ' A(% h % ."downElevator"\SE.seek :down A(>'X +73 h 'X ."downElevator"\SE.coast A(5 +27 (% h %X M&a% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'}& h & .tHand.open M& 1 +2 h 1 M&7& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&4(- +166 h (- M&N& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&W/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'<(W +416 h (W C';& h &W .tHand.open M&L1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'e% h % .t\CSV.dial CSR C'R  +38 h  C'[% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'2'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 ']% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'M'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'^& h &W C'P% h %4 .t\CSV.dial :flush C'I  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'c'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'm @ +37 h @ C'0% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  ',  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'\ % h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C'@'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  +38 h  .t\CBV.dial :waste2b C'd  +75 h  .t\CS.seek 0ml C'5'4 +766 h '4 #t\Flushing upper manifold C's& h &W C'S% h %4 .t\CSV.dial :flush C'p  +75 h  .t\CS.seek 5ml C':'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'J 'T  +38 h +75 .t\CTV.dial :air C'%  +151 h .t\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'h'4 +507 h '4 C'v& h &W C'V% h %4 .t\CSV.dial :flush C'j  +37 h  .t\CS.seek 3ml C'B'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +37 h @ C'% h %0 .t\CS.seek 5ml C'F'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C't'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'A'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'9'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'&& h &W C'-% h %4 .t\CSV.dial CSR C'3  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'?'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'Y' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'| +113 h .t\CTV.dial :intake C'6  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'\\' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'=  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'*  +113 h  C')% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'b'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +709 h ' ."connect"\CSV.dial CTV C'_  .t\CTV.dial CSV C'#  +38 h  +113  .t\CBV.dial :waste1 C'{ @ +37 h @ .t\CS.seek 0ml C'C'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'l  +75 h  .t\CS.seek 0ml C'`'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'z% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'O'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'Z  +37 h +76 .t\CTV.dial :air C'f  +150 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'} +37 h .t\CS.seek 0ml C' '4 +507 h '4 #t\Flushing upper manifold C'7& h &W C'% h %4 .t\CSV.dial :flush C'"  +37 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +37 h @ C'N% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'> +113 h .t\CTV.dial :air .\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'u'0 +507 h '0 .t\CSV.dial :waste C'x +37 h .t\CS.seek 0ml C'W'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'4'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'K'0 +507 h '0 .t\CSV.dial :waste C'; +75 h .t\CS.seek 0ml C'L'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'[ @ +38 h @ C'$% h %p .t\CS.seek 7ml C'k'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  '2  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'M'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'^& h &W C'P% h %4 .t\CSV.dial :flush C'I  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'm @ +37 h @ C'0% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C' '4 +766 h '4 #tDisinfecting external Intake with kill C'w& h &W C'% h %4 .t\CSV.dial CSR C'\  +38 h  C'g% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'D'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'/' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '@  +38 h +75 .t\CTV.dial :intake C'o  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'd' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 's'4 +606 h '4 .t\CSV.dial CSR C'S  +113 h  C'p% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'5'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'E' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C':'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&T& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +7 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'8( +416 h ( M&v& h &ӷ M&V% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &h(- +264 h (- M&U& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&F1 +150 h 1 M&t% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'A& h & .tHand.open M&&1 +2 h 1 M&-& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&?'η +90 h 'η .tHand.close M&|/ +150 h / M&6& h &- M&Y% h %η .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'_& h &W .tHand.open M +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'y% h %4 .t\CSV.dial CSR C'C  +113 h  C'!% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'l% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'z'4 +2009 h '4 #t\Purging external Intake C'`& h &W C'O% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f +75 h .t\CTV.dial :intake C'q  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'}  +75 h #t\Flushing upper manifold C'a& h &W C'7% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'"'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'H% h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +113 h .t\CTV.dial :air .\CS.seek 0ml C'>'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'Q +37 h .t\CS.seek 0ml C'x'4 +507 h '4 C'u& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'i'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C';'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C'$( +416 h ( M&R& h &ӷ M&]% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&k& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &P'η +91 h 'η .tHand.open M&I1 +150 h 1 M&M% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST15-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:19:36CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST15-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1417h1500ml.pcr F*0% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +87 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\ % h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*@'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +65 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*V'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1419h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *C'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*!  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*:' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*l  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Z  +108 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*a  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1419h1500ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*W% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*4% h %n ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*e  +65 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*P% h % ."MFBPCR"\AS.seek 0.81ml F*1'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*c% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1421h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +10 h  F*s% h %V ."MFBPCR"\AS.seek 0.5ml F*5'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*G  +22 h ."MFBPCR"delay 2 seconds +200 F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *<'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*v n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*U I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*V% h % ."MFBPCR"\AS.seek 0.53ml F*J' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*F % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*j'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*t  +107 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*- n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*9'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*B  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.65ml F*6' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F**'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*)  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1421h1500ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*=  +21 h ."MFBPCR"\ATV.dial :air3 F*_ % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*!'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*l'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *z'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*}  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*N'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +21 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*u% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V @CEST1539554418.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1500h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ARV.dial :carrier F*K  +10 h  F*\\% h %V ."MFBPCR"\AS.seek 0.5ml F*~'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *k'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*2 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*P% h %Fj ."MFBPCR"\AS.seek 0.524ml F*M' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*0 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*1' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*,  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*c% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*w n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*o' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*d% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*s % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1500h1500ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +21 h F*%% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*V% h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F*9 % +87 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*6% h % ."MFBPCR"\AS.seek 0.81ml F*)'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F**'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1502h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*O'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *f'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*}  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *7'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*H' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*( % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*N' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*"'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Q  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*4  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*['{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1502h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*e @@T ."MFBPCR"\ASV.dial :SPEdelivery F*~  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*b  +151 h F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *^' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*I  +43 h  F*L% h % ."MFBPCR"\AS.seek 0.62ml F*2'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*P n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *0'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*r'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*M % +43 h % F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *c' +424 h ' ."MFBPCR"\ATV.dial :air3 F*1% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*w' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*\ % h % H ."MFBPCR"\AS.seek 0.735ml F*g' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*D% h % Ű ."MFBPCR"\AS.seek 0.72ml F*@' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*d% h % zG ."MFBPCR"\AS.seek 0.365ml F*o' +7161 h ' ."MFBPCR"\ATV.dial :waste F*p n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *s'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*T% h % ."MFBPCR"\AS.seek 0.579ml F*5' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*G % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*U n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +22 h  ."MFBPCR"\AS.seek 0ml F*<'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*J % +108 h % F*.% h %V ."MFBPCR"\AS.seek 0.01ml F*t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*j% h % ."MFBPCR"\AS.seek 0.58ml F*-' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h % q ."MFBPCR"\AS.seek 0.605ml F*|'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*Y% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*B' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*? n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*6  +21 h  ."MFBPCR"\AS.seek 0ml F*h'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*)  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *=' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b **'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*8 n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +202 h 'R ."MFBPCR"\SPE.coast F*y  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*#'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*C% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *!' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*`  +43 h  F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*z  +65 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *O'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *f' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*} I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*a  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *'': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*7  +64 h  F*(% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *>'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*H n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *u' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*N I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *"': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*W n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*x n +150 h n ."MFBPCR"\AS.seek 0ml F*i'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*X  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*K'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %0 ."MFBPCR"\AS.seek 0.985ml F*[' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*]% h % ."MFBPCR"\AS.seek 0.97ml F*R'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *P'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *k'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*0  +43 h  F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *,' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *r' +9987 h ' ."MFBPCR"\ATV.dial AIV F*1 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*c' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*\  +22 h ."MFBPCR"\ATV.dial :air3 F*w % +42 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*T  +65 h  F*E% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *G'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*%' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*&% h %m ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*y n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b **'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*{ % +108 h % ."MFBPCR"\AS.seek 0ml F* 'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990525 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' 'C h  P% %# h  F* * h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *!  h  .t\SPE.configure SPEconfig F*\ 2 *:% h %V .t\AS.configure ASconfig S)`% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'Z& S)#.0X  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %N' +20 h '\  ."closeHand"Hand.close M&u/ .t\Elbow.jog 1500 M&* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b 'Q'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:20:35CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #15) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C']& h & .tHand.open M&[1 +150 h 1 M&& h &M M&R% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &I(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &P'η +91 h 'η .tHand.close M&2/ +150 h / M&0& h &- M&M% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'c& h &W .tHand.open M&1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C' % h %4 .t\CSV.dial CSR C'g  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '@% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'p'[ +463 h '[ .t\CSV.dial :waste C'd +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'S'4 +2009 h '4 #t\Purging external Intake C'/& h &W C'o% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'E .t\CBV.dial CSV C's  h +75 .t\CTV.dial :intake C'G  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'%'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'5  +75 h #t\Flushing upper manifold C'v& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'V'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'<% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'J +113 h .t\CTV.dial :air .\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'j'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.seek 3ml C't'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'-% h %0 .t\CS.seek 5ml C'Y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'|'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C'6 +75 h .t\CS.seek 0ml C'B'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&h& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&=/ +150 h / .t\CC.seek :open C'y( +416 h ( M&8& h &ӷ M&{% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &n'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 11:20:41CEST30-Sep-18\\n{1 => 22, 2 => 20, 3 => 2, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:20:35CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*;% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 3 into Collection Clamp #"readyMFB"Homing MFB Syringe C'e& ."readyMFB"\AFV.select :holdingCoil F* h & M&% h %\  A(C% h %X A(#. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*!% h %&K ."readyMFB"\ATV.dial :waste2 F*: % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *`' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (Z& h & M&l& h &- M&z% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +166 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &q' +90 h ' A(O& h & .t\SE.seek 0.71 Pucks below Top Plate A(}'K +253 h 'K A(f& h & M&& h & .t\Elbow.seek 3 M&a( +7 h ( M&& h & A(7& h & .t\Forearm.seek 3 + 300 counts M&('B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &H/ +150 h / ."downElevator"\SE.seek :down M& & A('X h & M&>% h %B .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.seek CC M&x(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' .t\CC.seek :closed C'\\(W +416 h (W .tHand.open M&~1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)i% h % C']& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'5J +216 h '5J ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV S)  +150 h  +100 .t\SSV.dial :spit,avoiding: :exhaust S)q +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C' 9 h 9Td +100 C'G9 h 9kd +100 C'9 h 9d +100 C'%9 h 9d +100 C'<9 h 9d +100 C'V9 h 9d +100 C'J9 h 9d +100 C'U9 h 9d +100 C'.9 h 9 d +100 C'S9 h 9 d +100 C'v9 h 96d +100 C'F9 h 9Ld +100 C'9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'j9 h 9d +100 C'99 h 9d +100 C'-9 h 9d +100 C'&9 h 9d +100 C't9 h 9d +100 C'|9 h 9d +100 C'69 h 9(d +100 C'h9 h 9>d +100 C'Y9 h 9Td +100 C'39 h 9id +100 C'A9 h 9d +100 C')9 h 9d +100 C'?9 h 9d +100 C'=9 h 9d +100 C'w9 h 9d +100 C'9 h 9d +100 C'{9 h 9  d +100 C'+9 h 9 d +100 C',9 h 9 *d +100 C'_9 h 9 ?d +100 C'm9 h 9 Td +100 C' 9 h 9 j d +100 C'89 h 9 $d +100 C'9 h 9 (d +100 C'*9 h 9 ,d +100 C';9 h 9 0d +100 C'9 h 9 4d +100 C'C9 h 9 8d +100 C'#9 h 9 9 h 9 d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'}9 h 9 d +100 C'(9 h 9 1d +100 C'a9 h 9 Dd +100 C'9 h 9 Xd +100 C'u9 h 9 kd +100 C'~9 h 9 ~d +100 C'y9 h 9 d +100 C'i9 h 9 d +100 C']9 h 9 d .t\CH.hold 5:00,85C C''   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'W @ +38 h @ .t\CS.seek 5ml C'N'p +1372 h 'p .t\CSV.dial CTV C'L  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'^ @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'`  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'[ @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'0'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'M +113 h .t\CBV.dial CSV C'  +19 h C'2% h %4 .t\CS.seek 5ml C'1'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'r  +37 h C'I% h %p .t\CS.seek 7ml C'k'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'b @ +38 h @ .t\CS.seek 10ml C'P' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'\ ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M& % h %\  A(g% h %X A(c. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&4& h &ӷ M&@% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &D( +143 h ( M&d& h & A(p& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &/' +89 h ' A(o& h &  .t\SE.seek 18.71 Pucks below Top Plate A(' +77 h ' M&& h & A(s& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &E' +26 h '  .t\Elbow.hold M&5, +6 h , .t\Elbow.reconfigure TightElbow A(T- h -+ M&$&.,(\ #b .t\SE.seek 19.04 Pucks below Top Plate A(B' +27 h ' M& % h %  A(G& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&%& h & A(<& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &V' +22 h ' .t\SE.seek 20.04 Pucks below Top Plate A(J' +37 h ' M&U& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +89 h '\  .t\Elbow.seek CC M&v(ӷ +142 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'j( +416 h ( A(9& h &  A(-& h &  M&&& h &ӷ M&t% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &6( +143 h ( M&h& h & A(Y& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &3' +92 h '  .t\Elbow.hold M&A, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&?% h %  A(=& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&w'8 +21 h '8 M&& h & A({& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +22 h ' A(,% h % ."downElevator"\SE.seek :down A(_'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (m% h %X M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +18 h @ .t\CS.seek 10ml C'*' +7 h ' .t\CSV.dial between air and CTV C'; ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'C& h & .tHand.open M +2 h 1 M&!& h & M&:% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&l& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.seek :garage M&n'η +91 h 'η .tHand.close M&e/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'7(W +416 h (W C'$& h &W .tHand.open M&H1 +150 h 1 M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%q  +38 h  .t\PS.seek 0ml P%>' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'}  P%(  h +76 .t\PSV.dial :mixloop P%a +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%u' +2609 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%y' +2610 h ' .t\PSV.dial :mixloop P%i +37 h .t\PS.seek 0ml P%]' +2610 h ' .t\PSV.dial :air P%'  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%R +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&`' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C'N( +416 h ( M&M& h &ӷ M&% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &0(- +264 h (- M&r& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &1'η +91 h 'η .tHand.open M&b1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &k'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'\ & h & M&% h %\  A( % h %X A(g. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (c( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&4& h &- M&@% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &d( +166 h ( M&p& h & A(o& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(s'\\ +264 h '\\ A(T& h & M& & h & .t\Elbow.seek 3 M&G( +7 h ( M&%& h & A(<& h & .t\Forearm.seek 3 + 300 counts M&"'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &U/ +150 h / ."downElevator"\SE.seek :down A(/'X M&.& h & M&D% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&E(ӷ +143 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +30 h 'X ."downElevator"\SE.coast A(5 +60 h ' .t\CC.seek :closed C'B(W +416 h (W .tHand.open M&V1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +151 h  .t\CSV.dial CTV C'\\  +19 h  .t\CS.seek 5ml C'9'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'-' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'&'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%t  +38 h  .t\PS.seek 2.5ml P%h' +2610 h ' .t\PSV.dial :mixloop P%Y +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%v +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%)  +150 h  .t\PS.seek :empty P%?' +2610 h ' .t\CBV.dial :waste1 C'= @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'{ @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C',  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C'6 @ +38 h @ .t\CS.seek 10ml C'm' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'; @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'C& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&#% h %\  A(!% h %X A(:. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (3( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&J& h &ӷ M&_% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &+( +142 h ( M&l& h & A(z& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(P& h &  .t\SE.seek 19.71 Pucks below Top Plate A(A' +65 h ' M&e& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +26 h '  .t\Elbow.hold M&8, +6 h , .t\Elbow.reconfigure TightElbow A(n- M&$&.,(\ #b h -+ .t\SE.seek 20.04 Pucks below Top Plate A(' +27 h ' M&f% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&O'8 +20 h '8 M&j& h & A($& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +23 h ' .t\SE.seek 21.04 Pucks below Top Plate A(Z' +36 h ' M&& h & M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&q(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&(/ +150 h / .t\CC.seek :open C'}( +416 h ( A(a& h &  A(& h &  M&& h &ӷ M&~% h % .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &i( +142 h ( M&y& h & A('& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &]' +93 h '  .t\Elbow.hold M&R, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&W% h %  A(L& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&^'8 +20 h '8 M&[& h & A(X& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(7% h % ."downElevator"\SE.seek :down A(M'X +54 h 'X ."downElevator"\SE.coast A(5 +46 (% h %X M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +90 h '\  .t\SC.coast .\PSV.dial :air A(6 P%I  +113 h  P%% h % .t\PS.seek 0.499ml P%b' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%2  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%\  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%g  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %4' +250 h ' .t\PRV.dial :waste2 P%@ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %1' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'p& h & .tHand.open M&o1 +2 h 1 M&& h & M&0% h %\  .t\Elbow.seek FlushPuck::Garage M&k(- +166 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&%'η +91 h 'η .tHand.close M&' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*q  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *W' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%L +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%[' +217 ."MFBPCR"\ATV.dial :AIV F*X I +43 h I F*7% h % ."MFBPCR"\AS.seek 0.469ml F*u' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%i +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%` +113 h P%]% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%^' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%Z  +37 h  .t\PS.seek 0ml P%I' +740 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*M n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*3'% +336 h ' .t\PSV.dial PSR P%? +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%\ ' +2610 h ' .t\PSV.dial PSR P% +113 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%b' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%@ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%4' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%o +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%0  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *R'V +850 h ' .t\PSV.dial :air P%<  +113 h  .t\PS.seek 2.5ml P%U' +2609 h ' .t\PSV.dial :mixloop P%. +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %r' +5107 h ' .t\PSV.dial :air P%%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %x' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*F  +43 h  F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *D'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*} I +43 h I F*"% h %Q ."MFBPCR"\AS.seek 0.01ml F*G' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %N' +618 h ' ."MFBPCR"\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +85 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *\\' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*1  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b **'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'c  +38 h  C'-% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C't'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'9  '  +37 h  +113  .t\CBV.dial :waste1 C'v @ +38 h @ .t\CS.seek 0ml C'Y'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*h  +21 h ."MFBPCR"delay 3 seconds +300 F*)% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'{  +38 h  C'=% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*6  +21 h F*,% h %ݏ ."MFBPCR"\AS.seek 0.419ml F* '& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*& n +108 h n F*;% h %& ."MFBPCR"\AS.seek 0.092ml F*m'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'C' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'!'4 +2648 h '4 C':& h &W #tGaraging FlushPuck in Collection Clamp M&w& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +6 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.seek CC M&J' +91 h ' .tHand.close M&P/ +150 h / .t\CC.seek :open C'z( +416 h ( M&& h &ӷ M&e% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &E(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'+& h & M&A% h %\  A( % h %X A(Q. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +357 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (O& h &,9 M&& h &- M&H% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&(& h &w A(B& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &,9 ."WCR"\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(8& h &,9 M&~& h &w ."WCR"\Elbow.seek 4 M&a(w +6 h (w M&'& h &w A(q& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +42 h '&0 ."MFBPCR"delay 3 seconds +108 h / ."downElevator"\SE.seek :down M&L& A(y'X h &w M&X% h %7 ."WCR"\Forearm.seek :retract M&7'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."WCR"\Elbow.seek CC M&>(ӷ +101 ."MFBPCR"\ATV.dial :waste2 F*W % +42 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &`' + h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *]'R +90 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&Z1 +54 h 'R ."MFBPCR"\ATV.dial :air2 F*^  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +75 h 1 M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)u+ +6 h + S)?% h % C'I& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'2  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'\ @ +38 h @ .t\CS.seek 0ml C'g'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'3  +38 h  C'b% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '@' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'`  +75 h  .t\CS.seek 0ml C'4'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'<  +38 h  C'%% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T 'o  +38 h +75 .t\CTV.dial :air C'0  +150 h .t\CS.seek 0ml C'"'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'p +37 h .t\CS.seek 0ml C'R'4 +507 h '4 #t\Flushing upper manifold C'k& h &W C'% h %4 .t\CSV.dial :flush C'V  +38 h  .t\CS.seek 5ml C'U'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'.% h %p .t\CS.seek 7ml C'G'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'1 +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'S'4 +506 h '4 C'\\& h &W C'-% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'c% h %0 .t\CS.seek 5ml C'5'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C')'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'h% h %4 .t\CSV.dial :flush C'Y  +37 h  .t\CS.seek 5ml C'='p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C', @ +37 h @ C'v% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CTV C'*  .t\CTV.dial CSV C'6  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'&% h %4 .t\CSV.dial :flush C'|  +76 h  .t\CS.seek 5ml C';'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'_ @ +38 h @ C':% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  +37 h  .t\CBV.dial :waste2b C'd  +75 h  .t\CS.seek 0ml C's'4 +766 h '4 #tDisinfecting external Intake with kill C'!& h &W C'/% h %4 .t\CSV.dial CSR C'P  +38 h  C'e% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'J' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +37 h +76 .t\CTV.dial :intake C'n  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'#  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +606 h '4 .t\CSV.dial CSR C'Q  +113 h  C'+% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'A'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C'H +113 h .t\CS.seek 0ml C' '4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&(& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C'~( +416 h ( M&f& h &ӷ M&'% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &q(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&X1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&y1 +2 h 1 M&i& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&z'η +91 h 'η .tHand.close M&Z/ +150 h / M&M& h &- M&7% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +264 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &]' +91 h ' .t\CC.seek :closed C'u(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &>'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'3& h &W C'b% h %4 .t\CSV.dial CSR C'g  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '`% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '@'[ +462 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '%'4 +2010 h '4 #t\Purging external Intake C'4& h &W C'F% h %4 .t\CSV.dial :flush C'o  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'0 .t\CBV.dial CSV +75 h .t\CTV.dial :intake C'r  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'"'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'p  +75 h #t\Flushing upper manifold C'& h &W C'k% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'V'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'% h %p .t\CS.seek 7ml C'.'i +415 h 'i ."connect"\CSV.dial CBV C'U .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'1'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'} +38 h .t\CS.seek 0ml C' '4 +506 h '4 C'I& h &W C'\\% h %4 .t\CSV.dial :flush C'-  +38 h  .t\CS.seek 3ml C'D'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +38 h @ C'9% h %0 .t\CS.seek 5ml C'c'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +6 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&,/ +150 h / .t\CC.seek :open C'v( +416 h ( M&=& h &ӷ M&*% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +91 h 'η .tHand.open M&|1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST16-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:22:26CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST16-Oct-18" +264322 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1517h1500ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*l  +22 h ."MFBPCR"\ATV.dial :air3 F*d % +87 h % F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*P% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*O  +31 h  ."MFBPCR"\ATV.dial :air3 F*8 % +87 h % F*t% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*f% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*y' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1519h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*~ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*3  +22 h ."MFBPCR"delay 2 seconds +200 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*]% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*`  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*<' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*@  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.536ml F*F'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*o  +108 h  ."MFBPCR"delay 7 seconds +700 F*%% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*r n +21 h n ."MFBPCR"delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.6ml F*p'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*R  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1519h1500ml.pcr F*1% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*G  +22 h ."MFBPCR"\ATV.dial :air3 F*I % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*S% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*D % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*5% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*h  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Y  +65 h  F*,% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *^'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*{ % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*$'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*l'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*w% h %m ."MFBPCR"\AS.seek 0.82ml F*;'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1521h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ARV.dial :carrier F*+  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*A'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *('R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*8  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*f n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*X % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*E'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*[  +107 h  ."MFBPCR"delay 7 seconds +700 F*W% h %< ."MFBPCR"\AS.seek 0.594ml F*y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*7  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*q  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*L % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1521h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*?  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *3'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*`'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*@'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *%'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*p  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*R% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*.'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*I% h %m ."MFBPCR"\AS.seek 0.82ml F*\\'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*}  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V @CEST1539640818.02 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1600h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*,  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*^% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*= n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*{  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *m'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*6 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*&% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*: n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*$' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*d  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.536ml F*l'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +107 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*P'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*#  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1600h1500ml.pcr F*Q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*H % +86 h % F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*X  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *['n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*7% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*\ 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *<'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1602h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ARV.dial :carrier F*@  +11 h  F*%% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*p  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *k'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*1% h % ."MFBPCR"\AS.seek 0.53ml F*.' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*V'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*}  +22 h  ."MFBPCR"delay 7 seconds +700 F*\\% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*S n +21 h n ."MFBPCR"delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*c  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*9% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*5  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*h% h % \\ ."MFBPCR"\AS.seek 0.326ml F*,'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*^ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1602h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*Y @@T ."MFBPCR"\ASV.dial :SPEdelivery F*>  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*u  +151 h F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*{ n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*|  +43 h  F*)% h % ."MFBPCR"\AS.seek 0.62ml F*6'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*& n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *:'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *w' +424 h ' ."MFBPCR"\ATV.dial :air3 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*/' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*l% h % ."MFBPCR"\AS.seek 0.71ml F*!' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +21 h % ."MFBPCR"delay 3 seconds +300 F*P% h % H ."MFBPCR"\AS.seek 0.735ml F*e' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*# n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*n' +7161 h ' ."MFBPCR"\ATV.dial :waste F*+ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*J% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*(% h % ."MFBPCR"\AS.seek 0.579ml F*A' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*8'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*O' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*'  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*B% h %V ."MFBPCR"\AS.seek 0.01ml F*[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +22 h F*E% h % ."MFBPCR"\AS.seek 0.58ml F*i' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h % q ."MFBPCR"\AS.seek 0.605ml F*Z'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*7% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*z n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*M  +21 h  ."MFBPCR"\AS.seek 0ml F*q'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *?' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *2'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*~ n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +202 h 'R ."MFBPCR"\SPE.coast F*b  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*3 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*4  +43 h  F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *@': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*%  +65 h  F*<% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *F'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*T n +65 h n F*r% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *0' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*p I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*k  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *1'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*G% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *I' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*. I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*\\  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*S n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *-'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*c'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*9  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*D % +43 h % F*5% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %0 ."MFBPCR"\AS.seek 0.985ml F*,' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F**% h % ."MFBPCR"\AS.seek 0.97ml F*='c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*{ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *)'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *&'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *m'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*:  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *d' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *C' +9987 h ' ."MFBPCR"\ATV.dial AIV F*$ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*w' +9880 h ' ."MFBPCR"\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.seek 0ml F*l'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*/% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*P  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +42 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +21 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*n'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*H  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*(  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +32 h  ."MFBPCR"\ATV.dial :air3 F*O % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*y% h %m ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *2'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*\ % +108 h % ."MFBPCR"\AS.seek 0ml F*3'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990525 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )] h  C' 'g h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ F*\ 9\ *\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 * % h %V .t\AS.configure ASconfig S)4% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'o& h & .t\CC.configure CCconfig S)#.0X& h &2 ."openPC"\PC.reconfigure PCconfig h &3 ."openPC"\PC.home.seek :open .t\CC.reconfigure CCconfig P%$x2 ^22b %( .t\CC.home.seek :open C'$x2 ^22b 'u( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 14:00:15 began in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:23:25CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #16) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&,1 +150 h 1 M&{& h &M M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &|(- +166 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &&'η +91 h 'η .tHand.close M&6/ +150 h / M&:& h &- M&% h %η .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C'/(W +416 h (W C'w& h &W .tHand.open M&l1 +150 h 1 M&P% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C's% h %4 .t\CSV.dial CSR C'e  +150 h  C'#% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '+'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Q'4 +2009 h '4 #t\Purging external Intake C'J& h &W C'n% h %4 .t\CSV.dial :flush C'H  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'j '  h +75 .t\CTV.dial :intake C't  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'A  +75 h #t\Flushing upper manifold C'f& h &W C'% h %4 .t\CSV.dial :flush C'8  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'O @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'B'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +507 h '0 .t\CSV.dial :waste C'W +37 h .t\CS.seek 0ml C'E'4 +507 h '4 C'& h &W C'a% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'['0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'7'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +507 h '0 .t\CSV.dial :waste C'M +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&?/ +150 h / .t\CC.seek :open C'b( +416 h ( M&~& h &ӷ M&\ % h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &v'η +91 h 'η .tHand.open M&1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:23:31CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 1, 4 => 21, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:23:25CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*_% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'Y& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&]% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(g% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (o& h &,9 M&`& h &- M&%% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &<(w +166 h (w M&@& h &w A(T& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &r' +89 h ' A(F& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(p' +66 h ' A(0& h &,9 M&"& h &w .t\Elbow.seek 4 M&(w +6 h (w M&& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&k'7 +23 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(G'X M&R& h &w M&1% h %7 .t\Forearm.seek :retract M&I'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M& (ӷ +142 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'^(W +416 h (W .tHand.open M&>1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)D+ +6 h + S)c% h % C'*& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\CS.reconfigure CSfast .\CS.seek :empty C'#d @b 'U' +710 h ' .t\CSV.dial CSR C'c  +150 h  C'*% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %  .t\CS.seek 4.5ml C'S'` +859 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'D  +38 h  .t\CS.seek 1.275ml C'=' +919 h ' #tHeating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'{   '.9 h 9d +100 C')9 h 9d +100 C'9 h 91d +100 C'49 h 9J d +100 C',9 h 9bd +100 C':9 h 9zd +100 C'9 h 9d +100 C'9 h 9d +100 C'69 h 9 d +100 C'$9 h 9$d +100 C'C9 h 9(d +100 C'|9 h 9 ,d +100 C'm9 h 9$0d +100 C'u9 h 9<4d +100 C'&9 h 9S8d +100 C'P9 h 9kd +100 C'9 h 9Td +100 C't9 h 9kd +100 C'89 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C''9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'B9 h 9 d +100 C'Q9 h 9 d +100 C'f9 h 96d +100 C'X9 h 9Ld +100 C'9 h 9bd +100 C'a9 h 9xd +100 C'O9 h 9d +100 C'E9 h 9d +100 C'9 h 9d +100 C'i9 h 9d +100 C'y9 h 9d +100 C'[9 h 9d +100 C'Z9 h 9d +100 C'M9 h 9(d +100 C'q9 h 9>d +100 C'79 h 9Td +100 C'9 h 9id +100 C'W9 h 9d +100 C' 9 h 9d +100 C'z9 h 9d +100 C'?9 h 9d +100 C'!9 h 9d +100 C'/9 h 9d +100 C'\ 9 h 9  d +100 C'K9 h 9 d +100 C'd9 h 9 *d +100 C'9 h 9 ?d +100 C'x9 h 9 Td +100 C'9 h 9 j d +100 C'~9 h 9 $d +100 C'L9 h 9 (d +100 C'29 h 9 ,d +100 C'_9 h 9 0d +100 C']9 h 9 4d +100 C'g9 h 9 8d +100 C'9 h 9 9 h 9 ~d +100 C'b9 h 9 d +100 C'c9 h 9 d +100 C'*9 h 9 d .t\CH.hold 5:00,85C C'U   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'D @ +113 h @ .t\CSV.dial :air C'S @ +38 h @ .t\CS.seek 5ml C'.'p +1372 h 'p .t\CSV.dial CTV C')  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'4  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C', @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C':'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'  +18 h C'6% h %4 .t\CS.seek 5ml C'$'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'C  +37 h C'|% h %p .t\CS.seek 7ml C'm'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'u @ +37 h @ .t\CS.seek 10ml C'&' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'P ` +132 h ` #tLysing Complete C' & h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&s% h %\  A(e% h %X A(w. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (l( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (;& h & M&5& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &J' +89 h ' A(n& h & .t\SE.seek -0.29 Pucks below Top Plate A(H'\\ +264 h '\\ M&#& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &j' +26 h ' .t\Elbow.hold M&A, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A((- h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&% h % A(t& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&8'B +20 h 'B M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'' +23 h ' .t\SE.seek 1.04 Pucks below Top Plate A(' +36 h ' M&& h & M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +89 h '\  .t\Elbow.seek CC M&f(ӷ +143 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&a' +91 h ' .tHand.close M&O/ +150 h / .t\CC.seek :open C'E( +416 h ( A(& h & A(i& h & M&y& h &ӷ M&[% h % .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &M( +142 h ( M&q& h & A(7& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&W, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&z% h % A(?& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&!'B +20 h 'B M&/& h & A(\ & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &K' +22 h ' A(d% h % ."downElevator"\SE.seek :down A('X +100 (x% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +90 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'L @ + A(6 +18 h @ .t\CS.seek 10ml C'2' +6 h ' .t\CSV.dial between air and CTV C'_ ` +19 h ` .t\AIV.dial :bypass C'] { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&1 +2 h 1 M&& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.seek :garage M&`'η +91 h 'η .tHand.close M&Y/ +150 h / M&@& h &- M&T% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'0& h &W .tHand.open M&R1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%1% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%<' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%r  +38 h  .t\PS.seek 0ml P%-' +1597 h ' .t\PSV.dial PSR P%h +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%\\' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%p  C'k  h +75 .t\PSV.dial :mixloop P%G +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%I  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%>  +37 h  .t\PS.seek 2.5ml P%b' +2610 h ' .t\PSV.dial :mixloop P%c +38 h .t\PS.seek 0ml P%*' +2609 h ' .t\PSV.dial :air P%U  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%= +37 h .t\PS.seek 0ml P%D' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&4' +91 h ' .tHand.close M&,/ +150 h / .t\CC.seek :open C'.( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +265 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &$'η +91 h 'η .tHand.open M&u1 +150 h 1 M&{% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'P& h & M& % h %\  A(s% h %X A(e. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (w(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (;& h &,9 M&5& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&+& h &w A(n& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(V& h &,9 .t\SE.seek 18.71 Pucks below Top Plate A(#' +77 h ' A(& h &,9 M&(& h &w .t\Elbow.seek 4 M&(w +7 h (w M&t& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&J& A(B'X h &w M&% h %7 .t\Forearm.seek :retract M&H'\ +77 h 'X ."downElevator"\SE.coast A(5 +14 h '\  .t\Elbow.seek CC M&j(ӷ +142 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&'1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'Q  +151 h  .t\CSV.dial CTV C'^  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'i' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'y'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%[  +38 h  .t\PS.seek 2.5ml P%q' +2609 h ' .t\PSV.dial :mixloop P%7 +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%a  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%f +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%z' +2610 h ' .t\CBV.dial :waste1 C'? @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'/  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'\ @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'd  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'Z ` +19 h ` .t\CSV.dial :air C'M @ +38 h @ .t\CS.seek 10ml C'x' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2648 h '4 .t\CBV.dial :waste1 C'L @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'!  +151 h C'_& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&]% h %\  A(g% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&& h &ӷ M&K% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &9( +143 h ( M&o& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &l' +89 h ' A(&& h & .t\SE.seek 0.71 Pucks below Top Plate A(W'K +254 h 'K M&Y& h & A(@& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &T' +25 h ' .t\Elbow.hold M&~, +7 h , .t\Elbow.reconfigure TightElbow A(`- M&$&.,(\ #b h -+K .t\SE.seek 1.04 Pucks below Top Plate A( ' +26 h ' M&"% h % A(N& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&F'B +21 h 'B M&E& h & A(0& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &R' +22 h ' .t\SE.seek 2.04 Pucks below Top Plate A(v' +36 h ' M&& h & M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&r(ӷ +143 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&p/ +150 h / .t\CC.seek :open C'k( +416 h ( A(G& h & A(I& h & M&\\& h &ӷ M&>% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +142 h ( M&b& h & A(U& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &*' +93 h ' .t\Elbow.hold M&=, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&S% h % A()& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&,& h & A(%& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +23 h ' A(3% h % ."downElevator"\SE.seek :down A('X +100 (% h %B M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%|  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%u' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%{ +38 h .t\PRV.dial :air P%6  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%P  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%s  +19 h  .t\PRV.dial :air P%e  +18 h  P%;% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %5' +251 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %$' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&n1 +2 h 1 M&V& h & M&:% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&m/ +150 h / M&& h &- M&J% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'#& h &W .tHand.open M&B1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  C'.& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%' @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%A  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%Q  +76 h  .tdelay 2 seconds +200 P%^% h % .t\PS.seek 1.499ml P%8' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%w' +1094 h ' .t\PSV.dial :air P%2  +38 h  .t\PS.seek 0ml P%' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%i +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%[  +38 h  .tdelay 2 seconds +200 P%D% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %7'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%a  +76 h .tdelay 2 seconds +200 P%f% h %r .t\PS.seek 0.475ml P% ' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%q  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%?'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%/  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%\  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %d' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Z +75 h .t\PTV.dial :PRV,avoiding: :puck P%M @ +75 h @ .t\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%x' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%L +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%!' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%_  +75 h  .t\PS.seek 2.5ml P%]' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%g +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'K & +50 h & .t\AWV.select :waste C' +7 P%o% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'& { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %j' +1313 h ' .t\PSV.dial :air P%O  +37 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .t\PSV.dial :PML P%@ +38 h .t\PS.seek 0ml P%`' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '"& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'N& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*E @@T *0  .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%1% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%l' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*9 I +129 h I F*W% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *T'6 +66 .t\PSV.dial :waste1 P%<  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%F +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%R +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%~' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*p  +22 h ' .t\PSV.dial PSR P% +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*G'ͯ +92 h P%I% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%\\' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*> % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%U  +37 h  .t\PS.seek 0ml P%-' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*r  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *S' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%,' +216 ."MFBPCR"\ATV.dial :AIV F*% I +43 h I F*3% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%c +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%4 +113 h P%*% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%v  +38 h  .t\PS.seek 0ml P%|' +739 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%{' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%6 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%P' +2609 h ' .t\PSV.dial PSR P% +113 h P%s% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%e' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +38 h  .t\PS.seek 0ml P%u' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%5' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%n +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %V'k +2679 h 'k .t\PSV.dial :waste1 P%:  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %(' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*t I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *='V +849 h ' .t\PSV.dial :air P%m  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%J +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %C' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*X  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*k I +43 h I F*#% h %Q ."MFBPCR"\AS.seek 0.01ml F*B' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %.' +619 h ' ."MFBPCR"\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.seek :empty F*'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*A  +86 h  F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *^' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*$  +43 h F*8% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *2'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'w  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'['P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'D' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'a  '  +38 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C'7'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*q  +22 h ."MFBPCR"delay 3 seconds +300 F* % h %_ ."MFBPCR"\AS.seek 0.434ml F*/'ݏ +110 h '4 .t\CSV.dial CSR C'\  +38 h  C'?% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'Z'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*M  +22 h F*d% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*y n +108 h n F*L% h %& ."MFBPCR"\AS.seek 0.092ml F*x'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'_' +1625 h ' ."connect"\CSV.dial CTV C'!  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'g'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&&/ +150 h / .t\CC.seek :open C'( +416 h ( M&O& h &ӷ M&j% h % .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +264 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M&1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'9& h & M&W% h %\  A(<% h %X A(}. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (l(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h &,9 M&h& h &- M&R% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b & (w +166 h (w M&p& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(I& h &,9 ."WCR"\SE.seek 17.71 Pucks below Top Plate A(T'* +86 h '* A(~& h &,9 M&>& h &w ."WCR"\Elbow.seek 4 M&G(w +6 h (w M&U& h &w A(r& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&'7 +23 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &\\/ +150 h / ."downElevator"\SE.seek :down A(b'X M&)& h &w M&%% h %7 ."WCR"\Forearm.seek :retract M&3'\ +18 h '&0 ."MFBPCR"delay 3 seconds +68 h 'X ."downElevator"\SE.coast A(5 +5 h '\  ."WCR"\Elbow.seek CC M&-(ӷ +142 h (ӷ M&S& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +85 ."MFBPCR"\ATV.dial :waste2 F*4 % +6 h ' ."WCR"\CC.seek :closed C'*(W +37 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +379 h (W ."WCR"Hand.open M&v1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +30 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +39 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)z% h % C'|& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'6  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*U' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*r n +86 h n ."MFBPCR"\ASV.dial :wasteR F*`  +43 h  ."MFBPCR"\AS.seek 0ml F*\\'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *%'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*S' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*c n +108 h n ."MFBPCR"\ASV.dial :wasteR F*T% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*b I +86 h I F*G% h % ."MFBPCR"\AS.seek 0.12ml F*3' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*x% h % ."MFBPCR"\AS.seek 0.13ml F*v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*z' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h % ."MFBPCR"\AS.seek 0.255ml F*,'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*l% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*s n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *e'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*{ @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*n'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*:  +43 h  #"MFBPCR"\Swish #1 of 2 F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *t'\  +1032 h '\  F*+% h %\  ."MFBPCR"\AS.seek 0.5ml F*m'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*5% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*C'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*X% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *(' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*J % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +3472 h 'V ."MFBPCR"\SPE.coast F*k  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1617h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*#  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +21 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*^  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*[  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*f % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*\ % h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*L  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*!% h % \\ ."MFBPCR"\AS.seek 0.326ml F*_'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226047 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'K  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'  +95 h  C'O% h % ."WCR"\CSV.dial between air and CTV C'H ` +18 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'"  'j  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ']'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'N' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'@  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'1  +37 h  C'W% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'}' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +815 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'9 @ +76 h @ +50 S)<% h % ."WCR"\CBV.dial between CSV and waste2b C'R  +94 h  C'0% h % ."WCR"\CSV.dial between air and CTV C'h ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'I  'p  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '~'4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'>  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Y' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'r  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'`  +38 h  C'U% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'\\' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'%  +94 h  C'c% h % ."WCR"\CSV.dial between air and CTV C'T ` +57 h ` ."WCR"\CS.seek 7.288ml C'S' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'b  '4  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'G'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'x  +150 h C'3& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(% h %X A(v. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (|( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (6& h & M&z& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &l( +143 h ( M&,& h & A(s& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(& h & ."WCR"\SE.seek 1.71 Pucks below Top Plate A(;': +244 h ': M&P& h & A(e& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' ."WCR"\Elbow.hold M&u, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(n- h -+: ."WCR"\SE.seek 2.04 Pucks below Top Plate A(:' +27 h ' M&V% h % A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&+'B +20 h 'B M&t& h & A(5& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &m' +23 h ' ."WCR"\SE.seek 3.04 Pucks below Top Plate A(' +36 h ' M&& h & M&X% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +89 h '\  ."WCR"\Elbow.seek CC M&J(ӷ +143 h (ӷ M&(& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ ."WCR"\Forearm.seek CC M&#' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(=& h & A(& h & M&& h &ӷ M&'% h % ."WCR"\Forearm.seek :retract M&A'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &B( +143 h ( M&$& h & A(8& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &Q' +92 h ' ."WCR"\Elbow.hold M&^, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&% h % A([& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&w& h & A(f& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &D' +22 h ' A(% h % ."downElevator"\SE.seek :down A( 'X +100 (\ % h %1 M&a% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +90 h '\  +42 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&?1 +2 A(6 h 1 M&d& h & M&M% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +166 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&L'η +91 h 'η .tHand.close M&!/ +150 h / M&y& h &- M&Z% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'& h &W .tHand.open M&O1 +150 h 1 M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'j& h &W C'"% h % .t\CSV.dial CSR C'  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '@% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '1'[ +462 h '[ .t\CSV.dial :waste C'W +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'N'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'}& h &W C'9% h %4 .t\CSV.dial :flush C'<  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'R'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'h% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'p  'I  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'>'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'~& h &W C'r% h %4 .t\CSV.dial :flush C'`  +75 h  .t\CS.seek 5ml C'U'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Y @ +37 h @ C'\\% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'%  +75 h  .t\CS.seek 0ml C'c'4 +766 h '4 #t\Flushing upper manifold C'T& h &W C')% h %4 .t\CSV.dial :flush C'b  +75 h  .t\CS.seek 5ml C'4'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +37 h @ C'% h %p .t\CS.seek 7ml C'G'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 'x  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +506 h '0 .t\CSV.dial :waste C'6 +38 h .t\CS.seek 0ml C'z'4 +506 h '4 C'& h &W C',% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +37 h @ C'% h %0 .t\CS.seek 5ml C'P'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'e'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +506 h '0 .t\CSV.dial :waste C'V +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C't& h &W C'5% h %4 .t\CSV.dial CSR C'  +151 h  C'X% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C' 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +416 h ' ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'(' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'k  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '+'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C':  +113 h  C'm% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'u'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  '*  +38 h  +113  .t\CBV.dial :waste1 C'= @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C''% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '$'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'#  +38 h  .t\CBV.dial :waste2b C'J  +75 h  .t\CS.seek 0ml C'i'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'[% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'A  +37 h +76 .t\CTV.dial :air C'B  +150 h .t\CS.seek 0ml C'\ '4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +506 h '0 .t\CSV.dial :waste C'7 +38 h .t\CS.seek 0ml C'?'4 +507 h '4 #t\Flushing upper manifold C'd& h &W C'M% h %4 .t\CSV.dial :flush C'Q  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'/% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'D .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'|'0 +507 h '0 .t\CSV.dial :waste C'^ +37 h .t\CS.seek 0ml C'!'4 +507 h '4 C'y& h &W C'Z% h %4 .t\CSV.dial :flush C'q  +37 h  .t\CS.seek 3ml C'L'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'2'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'O'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'H& h &W C'j% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'@  .t\CTV.dial CSV C']  +38 h  +113  .t\CBV.dial :waste1 C'W @ +37 h @ .t\CS.seek 0ml C'1'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'}& h &W C'9% h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C'N'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'h% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'I  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'F& h &W C'~% h %4 .t\CSV.dial CSR C'r  +38 h  C'`% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'>'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'Y' +416 h ' ."connect"\CSV.dial CBV C'\\ .t\CBV.dial CSV C'U  +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'%' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'T'4 +606 h '4 .t\CSV.dial CSR C')  +113 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'c'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'S' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'4'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&6/ +150 h / .t\CC.seek :open C'v( +416 h ( M&& h &ӷ M&,% h % .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &z(- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&P1 +150 h 1 M&e% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&t1 +2 h 1 M&5& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&;/ +150 h / M&& h &- M&l% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +265 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'u(W +416 h (W C'C& h &W .tHand.open M&*1 +150 h 1 M&=% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C''% h %4 .t\CSV.dial CSR C'  +112 h  C'$% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'#'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'J% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '8'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '['4 +2009 h '4 #t\Purging external Intake C'i& h &W C'w% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'B +75 h .t\CTV.dial :intake C'f  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'\ '4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'7  +75 h #t\Flushing upper manifold C'a& h &W C'd% h %4 .t\CSV.dial :flush C'M  +75 h  .t\CS.seek 5ml C'Q'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +37 h @ C'% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'D'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'^'4 +507 h '4 C'|& h &W C'y% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.seek 3ml C'q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'L'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'2'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.seek CC M&O' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&@% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &9'η +91 h 'η .tHand.open M&<1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST17-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:125\\n{1 => 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:25:14CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST17-Oct-18" +263647 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1617h1500ml.pcr F*h% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F*I % +86 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*r% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*U'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*%  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*T' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*G  +32 h  ."MFBPCR"\ATV.dial :air3 F*6 % +87 h % F*K% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*P% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*t' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *V'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1619h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*:  +11 h  F*m% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *C'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*=  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*$  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*+% h %Fj ."MFBPCR"\AS.seek 0.524ml F*4' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*J  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*8  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +108 h  ."MFBPCR"delay 7 seconds +700 F*[% h %< ."MFBPCR"\AS.seek 0.594ml F*B'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*7'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*a  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*?  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*/ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1619h1500ml.pcr F*D% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +21 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*L'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*j  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*}  +31 h  ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*9% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +21 h % ."MFBPCR"delay 3 seconds +300 F*_% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +21 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *r'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1621h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*Y  +43 h  ."MFBPCR"\ARV.dial :carrier F*b  +10 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F*c'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*6  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*K  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*&% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*- I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.53ml F*3' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*s'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*e  +108 h  ."MFBPCR"delay 7 seconds +700 F*n% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +21 h n ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*V % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1621h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*k  +22 h ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*$'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +21 h F*4% h %n ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*i  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*A% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *['n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*7  +31 h  ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*|% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V @CEST1539727220.71 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1700h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*}  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*H  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*] I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*1' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*h n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*I  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*~  +108 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*r'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*F n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*>'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*`  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*%% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Y'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1700h1500ml.pcr F*)% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*x% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*o'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*-  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*n' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*C% h %m ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *''R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1702h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*A  +43 h  ."MFBPCR"\ARV.dial :carrier F*8  +11 h  F*[% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*7  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\  +21 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*M I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*{ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*^'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +22 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*L  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*j% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1702h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*" @@T *  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*u  +151 h F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *}' +423 h ' ."MFBPCR"\ATV.dial :ASV F*H n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*<  +43 h  F*O% h % ."MFBPCR"\AS.seek 0.62ml F*]'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*9 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *h'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*_'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*1 % +43 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *R' +423 h ' ."MFBPCR"\ATV.dial :air3 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +21 h F*p% h % ."MFBPCR"\AS.seek 0.71ml F*F' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h % H ."MFBPCR"\AS.seek 0.735ml F*`' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F*>% h % Ű ."MFBPCR"\AS.seek 0.72ml F*U' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*%% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*b n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*) % +43 h % F*Y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*x% h % ."MFBPCR"\AS.seek 0.579ml F*c' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*&% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*G' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*- n +86 h n ."MFBPCR"\ASV.dial :wasteR F*,  +22 h  ."MFBPCR"\AS.seek 0ml F*o'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*3 % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*e' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*s% h % ."MFBPCR"\AS.seek 0.58ml F*5' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h % q ."MFBPCR"\AS.seek 0.605ml F*;'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*l% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*z'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *k' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*C n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *:'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*v n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *='R +202 h 'R ."MFBPCR"\SPE.coast F*'  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F**'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*+  +42 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *$' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*# I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*i  +43 h  F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *8': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*[  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *w'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*f% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *B' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*a  +43 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*d  +64 h  F*{% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *D'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *|' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*/ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*y  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*! n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *Z'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*^ n +151 h n ."MFBPCR"\AS.seek 0ml F*g'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*q % +43 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F* % h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*@% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *9'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*1% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *I' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *_' +9988 h ' ."MFBPCR"\ATV.dial AIV F*E I +43 h I ."MFBPCR"\AS.seek 0.02ml F*R' +9880 h ' ."MFBPCR"\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.seek 0ml F*p'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*~% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*r  +21 h ."MFBPCR"\ATV.dial :air3 F*F % +43 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *>'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*%'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*Y% h %n ."MFBPCR"\AS.seek 0.82ml F*T'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +64 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*o% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*e'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *l'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *k'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*' n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *:'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*= % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990256 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )+ h  C' ' h  P% %* h  F* *# h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *$  h  .t\SPE.configure SPEconfig F*\ 2 *4% h %V .t\AS.configure ASconfig S)i% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'A& S)#.0X 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:26:13CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #17) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'@& h & .tHand.open M&1 +150 h 1 M&H& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &<(- +166 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &9'η +91 h 'η .tHand.close M&]/ +150 h / M&h& h &- M&1% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +264 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &N' +91 h ' .t\CC.seek :closed C'~(W +416 h (W C'R& h &W .tHand.open M&p1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'\\& h &W C'% h %4 .t\CSV.dial CSR C'`  +150 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '>'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'U% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'b'[ +463 h '[ .t\CSV.dial :waste C'% +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ')'4 +2009 h '4 #t\Purging external Intake C'Y& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T 'S  h +75 .t\CTV.dial :intake C'K  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'c  +75 h #t\Flushing upper manifold C'& h &W C'&% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C','p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +37 h @ C'o% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C'n +37 h .t\CS.seek 0ml C's'4 +507 h '4 C'& h &W C'X% h %4 .t\CSV.dial :flush C't  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C';'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +7 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&C' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C''( +416 h ( M&v& h &ӷ M&=% h % .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +264 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:26:18CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 4, 4 => 18, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:26:13CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*0% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'"& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&+% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(*. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (#(,9 +6 F*$% h %&K ."readyMFB"\ATV.dial :waste2 F*4 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *i' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &,9 M&J& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&8& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &f' +89 h ' A(w& h &,9 .t\SE.seek 16.71 Pucks below Top Plate A(7'; +96 h '; A(B& h &,9 M&\ & h &w .t\Elbow.seek 4 M&a(w +6 h (w M&M& h &w A({& h &,9 .t\Forearm.seek 4 + 300 counts M&d'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&?& h &w A('X M&D% h %7 .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.seek CC M&^(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C'((W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)q+ +6 h + S)g% h % C'@& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  h +1447 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'%% h %4 S)b% h % S)Y% h % .tIntake.select :close S)0 +6 )% h % .t\SSV.dial :exhaust S)  +75 h  .t\Exhaust.select :open S)@ +7 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9@`d +100 C'%9 h 9Wdd +100 C'b9 h 9nhd +100 C'Y9 h 9ld +100 C'9 h 9pd +100 C'9 h 9td +100 C' 9 h 9xd +100 C'S9 h 9|d +100 C'T9 h 9d +100 C'c9 h 9d +100 C'x9 h 9'd +100 C'9 h 9>d +100 C'&9 h 9Td +100 C'K9 h 9kd +100 C'69 h 9d +100 C'9 h 9d +100 C'o9 h 9d +100 C',9 h 9d +100 C'39 h 9d +100 C'-9 h 9d +100 C'9 h 9 d +100 C')9 h 9 d +100 C'9 h 96d +100 C'P9 h 9Ld +100 C'9 h 9bd +100 C'X9 h 9xd +100 C'G9 h 9d +100 C's9 h 9d +100 C'9 h 9d +100 C'59 h 9d +100 C't9 h 9d +100 C'e9 h 9d +100 C';9 h 9d +100 C'9 h 9(d +100 C'z9 h 9>d +100 C'l9 h 9Td +100 C'9 h 9id +100 C'n9 h 9d +100 C'm9 h 9d +100 C' 9 h 9d +100 C'k9 h 9d +100 C'F9 h 9d +100 C'~9 h 9d +100 C'=9 h 9  d +100 C'.9 h 9 d +100 C'I9 h 9 *d +100 C'C9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C'v9 h 9 $d +100 C'V9 h 9 (d +100 C':9 h 9 ,d +100 C'09 h 9 0d +100 C'+9 h 9 4d +100 C'9 h 9 8d +100 C'*9 h 9 ( +142 h ( M&%& h & A(b& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &Y' +90 h ' A(& h & .t\SE.seek 2.71 Pucks below Top Plate A(') +234 h ') M& & h & A(S& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &T' +26 h ' .t\Elbow.hold M&c, +6 h , .t\Elbow.reconfigure TightElbow A(x- h -+) M&$&.,(\ #b .t\SE.seek 3.04 Pucks below Top Plate A(' +27 h ' M&&% h % A(K& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&6'B +20 h 'B M&& h & A(o& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &,' +23 h ' .t\SE.seek 4.04 Pucks below Top Plate A(3' +36 h ' M&-& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&X' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C's( +416 h ( A(& h & A(5& h & M&t& h &ӷ M&e% h % .t\Forearm.seek :retract M&;'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&z& h & A(l& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&n, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &m1 +150 h 1 M& % h % A(k& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&F'B +20 h 'B M&~& h & A(=& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &.' +22 h ' A(I% h % ."downElevator"\SE.seek :down A(C'X +100 (% h %  M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &v'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'V @ +2 A(6 +17 h @ .t\CS.seek 10ml C':' +6 h ' .t\CSV.dial between air and CTV C'0 ` +19 h ` .t\AIV.dial :bypass C'+ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&*1 +2 h 1 M&$& h & M&4% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.seek :garage M&J'η +91 h 'η .tHand.close M&"/ +150 h / M&8& h &- M&% h %η .t\Forearm.seek :retract M&w'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&W& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'B& h &W .tHand.open M&?1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%{ +151 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%f  +38 h  .t\PS.seek 0ml P%Z' +1597 h ' .t\PSV.dial PSR P%j +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%d  C'7  h +75 .t\PSV.dial :mixloop P%a +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%|  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%'' +2609 h ' .t\PSV.dial :mixloop P%g +38 h .t\PS.seek 0ml P%@' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%#' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%q' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&!& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +7 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'/( +416 h ( M&1& h &ӷ M& % h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &h(- +264 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &E'η +91 h 'η .tHand.open M&u1 +150 h 1 M&H% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'r& h & M&\\% h %\  A(% h %X A(`. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (R(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (N& h &,9 M&2& h &- M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &%(w +166 h (w M&b& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &^' +89 h ' A(Q& h &,9 .t\SE.seek 15.71 Pucks below Top Plate A( 'L +107 h 'L A(S& h &,9 M&x& h &w .t\Elbow.seek 4 M&&(w +7 h (w M&K& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&o'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &-/ +150 h / ."downElevator"\SE.seek :down M&Y& A('X h &w M&>% h %7 .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&T(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&P& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&,1 +150 h 1 M&c% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C')  +151 h  .t\CSV.dial CTV C'(  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'5' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C't'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%e  +38 h  .t\PS.seek 2.5ml P%z' +2609 h ' .t\PSV.dial :mixloop P%l +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%X  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%m  +151 h  .t\PS.seek :empty P% ' +2610 h ' .t\CBV.dial :waste1 C'k @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'~  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'= @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'I  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'; ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'V'4 +2648 h '4 .t\CBV.dial :waste1 C' @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'0  +151 h C'+& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&% h %\  A(*% h %X A($. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (4( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (F& h & M&3& h &ӷ M&.% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&A& h & A(p& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &C' +89 h ' A(9& h & .t\SE.seek 3.71 Pucks below Top Plate A(n' +224 h ' M&"& h & A(8& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +25 h ' .t\Elbow.hold M&v, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(J- h -+ .t\SE.seek 4.04 Pucks below Top Plate A(W' +27 h ' M&\ % h % A( & h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &w'B +21 h 'B M&s& h & A(B& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &?' +22 h ' .t\SE.seek 5.04 Pucks below Top Plate A(' +36 h ' M&{& h & M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +90 h '\  .t\Elbow.seek CC M&f(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&Z' +91 h ' .tHand.close M&d/ +150 h / .t\CC.seek :open C'7( +416 h ( A(a& h & A(|& h & M&y& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &g( +143 h ( M&'& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &@' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b  +150 h 1 M&!% h % A(O& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&}'B +21 h 'B M&& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(1'X +100 ( % h % M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%<  +2 A(6 +111 h  P%L% h % .t\PS.seek 0.499ml P%u' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%H +37 h .t\PRV.dial :air P%]  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%r  +38 h .t\CBV.dial AIV C'\\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%`  +19 h  P%N% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %2' +250 h ' .t\PRV.dial :waste2 P%U @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %E' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'b& h & .tHand.open M&1 +2 h 1 M&Q& h & M&h% h %\  .t\Elbow.seek FlushPuck::Garage M&S(- +165 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&-& h &- M&Y% h %η .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &%(ӷ +265 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'o& h &W .tHand.open M&&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'/& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%, @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%c  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%)  +75 h  .tdelay 2 seconds +200 P%(% h % .t\PS.seek 1.499ml P%6' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%R' +1094 h ' .t\PSV.dial :air P%:  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%5 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%e  +37 h  .tdelay 2 seconds +200 P%q% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %l'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%X  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%m' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%z  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%k'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%~  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%=  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %I' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%; +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%t' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%0' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%+  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%* +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%$  +76 h P%F% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %3' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'. & +50 h & .t\AWV.select :waste C' +6 P%A% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %p'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'9 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' .t\PSV.dial :air P%G  +38 h  .t\PS.seek 2.5ml P%"' +2610 h ' .t\PSV.dial :PML P%8 +37 h .t\PS.seek 0ml P%J' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\ & h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C' & F*s @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*B  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%{ +22 h  ."MFBPCR"delay 20 seconds +91 h P%D% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%C' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*n% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%j' +1598 h ' .t\PSV.dial PSR P%w +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%W' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%v' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*d  +22 h ' .t\PSV.dial PSR P%M +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*a'ͯ +92 h P%|% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%y' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%Z' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*f  +22 h  F*#% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *!' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%O +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%g +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P%i +113 h P%@% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%}' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%<' +739 h ' ."MFBPCR"delay 5 seconds +500 F*L9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*1% h % ."MFBPCR"\AS.seek 0.041ml F*4'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%H' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%] +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial PSR P%\\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%`' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%N  +37 h  .t\PS.seek 0ml P%u' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%2' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %Q'k +2678 h 'k .t\PSV.dial :waste1 P%h  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %x' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*K I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%-' +2610 h ' .t\PSV.dial :mixloop P%Y +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %_' +5107 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %^' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*P  +43 h  F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *>'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*7 I +43 h I F*o% h %Q ."MFBPCR"\AS.seek 0.01ml F*&' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %/' +618 h ' ."MFBPCR"\ATV.dial :waste F*, n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*c  +86 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *(' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*E  +43 h F*6% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *:'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'R  +37 h  C'5% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'e'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'l'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*z  +22 h ."MFBPCR"delay 3 seconds +300 F*m% h %_ ."MFBPCR"\AS.seek 0.434ml F*~'ݏ +110 h '4 .t\CSV.dial CSR C'=  +37 h  C'k% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C';'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*I% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*t n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'+' +1625 h ' ."connect"\CSV.dial CTV C'0  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'*'4 +2647 h '4 C'$& h &W #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&.& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.seek CC M&3' +90 h ' .tHand.close M&9/ +150 h / .t\CC.seek :open C'p( +416 h ( M&G& h &ӷ M&T% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &"(- +265 h (- M&\ & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&{1 +150 h 1 M&D% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'& h & M&n% h %\  A(% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (C(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (w& h &,9 M&j& h &- M&?% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &W(w +166 h (w M&d& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &M' +89 h ' A(|& h &,9 ."WCR"\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(v& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M&a(w +7 h (w M&& h &w A(f& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&#'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &y/ +138 h '&0 ."MFBPCR"delay 3 seconds +12 h / ."downElevator"\SE.seek :down A(''X M&O& h &w M&[% h %7 ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&Z(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&!& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +55 ."MFBPCR"\ATV.dial :waste2 F*i % +36 h ' ."WCR"\CC.seek :closed C'@(W +7 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +409 h (W ."WCR"Hand.open M&1 +150 h 1 M&}% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ + h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *1' +68 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S) % h % C'<& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C']  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +75 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*1 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*(  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*R' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*e  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*V' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.536ml F*X'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +107 h  ."MFBPCR"delay 7 seconds +700 F*=% h %< ."MFBPCR"\AS.seek 0.594ml F*k'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*z n +21 h n ."MFBPCR"delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*t' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*0% h % \\ ."MFBPCR"\AS.seek 0.326ml F*+'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*;  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*F % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226077 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'.  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'$ @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'% @ +76 h @ +50 S)A% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'G% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'3' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\  'T  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'{  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'8  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'D  +38 h  C'n% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'?  +94 h  C'B% h % ."WCR"\CSV.dial between air and CTV C'j ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'd  '|  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'v'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '"' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'f  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'J  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'y' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'O' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'W @ +75 h @ +50 S)M% h % ."WCR"\CBV.dial between CSV and waste2b C'[  +94 h  C'g% h % ."WCR"\CSV.dial between air and CTV C'# ` +57 h ` ."WCR"\CS.seek 7.288ml C'!' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'a  'i  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&}% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (<( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (]& h & M& & h &ӷ M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &C( +142 h ( M&& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\\' +90 h ' A(& h & ."WCR"\SE.seek 4.71 Pucks below Top Plate A(N' +214 h ' M&r& h & A(`& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &4' +25 h ' ."WCR"\Elbow.hold M&u, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 5.04 Pucks below Top Plate A(h' +27 h ' M&Q% h % A(U& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&b'B +21 h 'B M&K& h & A(2& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 6.04 Pucks below Top Plate A(' +36 h ' M&-& h & M&P% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +90 h '\  ."WCR"\Elbow.seek CC M&Y(ӷ +142 h (ӷ M&x& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ ."WCR"\Forearm.seek CC M&o' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'>( +416 h ( A(& h & A(S& h & M&1& h &ӷ M&,% h % ."WCR"\Forearm.seek :retract M&c'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &&( +143 h ( M&E& h & A(6& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &)' +92 h ' ."WCR"\Elbow.hold M&(, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &51 +150 h 1 M&% h % A(e& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&R& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &q' +22 h ' A(V% h % ."downElevator"\SE.seek :down A(m'X +100 (=% h % M&X% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'l& h & .tHand.open M&k1 + A(6 + h 1 M&I& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&:(- +166 h (- M&~& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&0/ +150 h / M&t& h &- M&;% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +264 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'A(W +416 h (W C' & h &W .tHand.open M&G1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'T& h &W C'\ % h % .t\CSV.dial CSR C'3  +38 h  C'{% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '8% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'D'[ +463 h '[ .t\CSV.dial :waste C'n +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '?'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +37 h @ C'j% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV C'd  .t\CTV.dial CSV C'|  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'v& h &W C'f% h %4 .t\CSV.dial :flush C'J  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +37 h @ C'y% h %p .t\CS.seek 7ml C'W'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +38 h  .t\CBV.dial :waste2b C'[  +75 h  .t\CS.seek 0ml C'g'4 +766 h '4 #t\Flushing upper manifold C'#& h &W C'O% h %4 .t\CSV.dial :flush C'i  +75 h  .t\CS.seek 5ml C'a'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +37 h @ C'% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'}  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'] +38 h .t\CS.seek 0ml C' '4 +506 h '4 C' & h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'L% h %0 .t\CS.seek 5ml C'r'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'`'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'Q +75 h .t\CS.seek 0ml C'U'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'K& h &W C'2% h %4 .t\CSV.dial CSR C'-  +151 h  C'P% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'\\'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'C' +416 h ' ."connect"\CSV.dial CBV C'N .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'4  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'x' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'7  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'h  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'u'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  '@  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'S'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'1  +38 h  C',% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'E'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  +38 h  .t\CBV.dial :waste2b C'Y  +75 h  .t\CS.seek 0ml C'5'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'e% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'R'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'V 'c  +37 h +76 .t\CTV.dial :air C'&  +150 h .t\CS.seek 0ml C'='4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +506 h '0 .t\CSV.dial :waste C'l +38 h .t\CS.seek 0ml C'k'4 +507 h '4 #t\Flushing upper manifold C'I& h &W C'% h %4 .t\CSV.dial :flush C')  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'~% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'q .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'( +37 h .t\CS.seek 0ml C'0'4 +507 h '4 C't& h &W C';% h %4 .t\CSV.dial :flush C'z  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'*% h %0 .t\CS.seek 5ml C':'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'F'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'>'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'G'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'T% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.seek 5ml C'3'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'%% h %p .t\CS.seek 7ml C'p'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'8  '  +38 h  +112  .t\CBV.dial :waste1 C'n @ +38 h @ .t\CS.seek 0ml C'D'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'B @ +37 h @ C'j% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  +38 h  .t\CBV.dial :waste2b C'|  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'w& h &W C'v% h %4 .t\CSV.dial CSR C'f  +38 h  C'J% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'"' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'y '  +38 h +75 .t\CTV.dial :intake C'M  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'[' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'W  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +607 h '4 .t\CSV.dial CSR C'O  +113 h  C'i% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'g'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'!' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'a'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&]/ +150 h / .t\CC.seek :open C'( +416 h ( M& & h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&Z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&r1 +150 h 1 M&`% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&K1 +2 h 1 M&2& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &-(- +6 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&\\'η +91 h 'η .tHand.close M&N/ +150 h / M&4& h &- M&C% h %η .t\Forearm.seek :retract M&7'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +265 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &b' +91 h ' .t\CC.seek :closed C'u(W +416 h (W C'_& h &W .tHand.open M&@1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'1& h &W C',% h %4 .t\CSV.dial CSR C'S  +113 h  C'E% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'o'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'Y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '6'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'e'4 +2009 h '4 #t\Purging external Intake C'5& h &W C'R% h %4 .t\CSV.dial :flush C'c  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'='4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'l  +75 h #t\Flushing upper manifold C'X& h &W C'I% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C')'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +37 h @ C'% h %p .t\CS.seek 7ml C'~'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'm +37 h .t\CS.seek 0ml C'('4 +507 h '4 C'<& h &W C't% h %4 .t\CSV.dial :flush C';  +37 h  .t\CS.seek 3ml C'z'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'$% h %0 .t\CS.seek 5ml C'*'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +506 h '0 .t\CSV.dial :waste C'F +76 h .t\CS.seek 0ml C' '4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&>& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&T& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.seek CC M&G' +91 h ' .tHand.close M&{/ +150 h / .t\CC.seek :open C'%( +416 h ( M&3& h &ӷ M&8% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &n(- +264 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&D% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST18-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:130\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:28:01CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST18-Oct-18" +263677 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1717h1500ml.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*d  +21 h ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*J'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*W  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*O% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +32 h  ."MFBPCR"\ATV.dial :air3 F*] % +86 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*L'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1719h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ARV.dial :carrier F*h  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*E  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*b% h %Fj ."MFBPCR"\AS.seek 0.524ml F*a' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*Y  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*6  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +107 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*l'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*~ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1719h1500ml.pcr F*q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*0% h %Z ."MFBPCR"\AS.seek 0.81ml F*$'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*:% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*T  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\  +65 h  F*{% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *x'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*s'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*d'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1721h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*i  +11 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*g'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*]  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *A'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*Z I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*r % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*`  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.65ml F*4' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\\% h % \\ ."MFBPCR"\AS.seek 0.326ml F*h'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1721h1500ml.pcr F*-% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*7  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*E'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*a% h %n ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*l  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V @CEST1539813620.12 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1800h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *p'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*D' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*j n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*s' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*|  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.536ml F*d'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*w n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*[% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1800h1500ml.pcr F*O% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*!  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*}% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*r  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*L  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *`'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*N  +32 h  ."MFBPCR"\ATV.dial :air3 F*U % +86 h % F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*4% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +21 h % ."MFBPCR"delay 3 seconds +300 F*-% h %> ."MFBPCR"\AS.seek 0.835ml F*h'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +21 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1802h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*6  +10 h  F*e% h %V ."MFBPCR"\AS.seek 0.5ml F*R'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*l  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*H % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*~' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*m  +21 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*('\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*0 n +21 h n ."MFBPCR"delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.6ml F*z'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*F' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*:  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*T% h % \\ ."MFBPCR"\AS.seek 0.326ml F*{'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1802h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*\ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*u  +150 h F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*G% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2034 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *j'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*+'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*D % +43 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *?' +424 h ' ."MFBPCR"\ATV.dial :air3 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*d% h % ."MFBPCR"\AS.seek 0.71ml F*w' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h % H ."MFBPCR"\AS.seek 0.735ml F*J' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*W n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F*[% h % zG ."MFBPCR"\AS.seek 0.365ml F*M' +7162 h ' ."MFBPCR"\ATV.dial :waste F*i n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*O % +42 h % F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *!' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*g' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h %  ."MFBPCR"\AS.seek 0.604ml F*]'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*9% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*'' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Z n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *L'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*`' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*2' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h % q ."MFBPCR"\AS.seek 0.605ml F*N'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*C% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*4  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *-'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *7' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *h'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +201 h 'R ."MFBPCR"\SPE.coast F*,  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*@'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*1 n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*b  +43 h  F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *E' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*o I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*5  +43 h  F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*e  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *R'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*V n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *&' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*l I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*X  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*I  +65 h  F*H% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *q'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*~ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*t  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *)': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*0 n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *;'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*( n +151 h n ."MFBPCR"\AS.seek 0ml F**'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*$  +85 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*z % +43 h % F*:% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +21 h F*F% h % ."MFBPCR"\AS.seek 0.96ml F*>'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*{' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +21 h F*8% h % ."MFBPCR"\AS.seek 0.97ml F*3'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *G'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *p'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*j  +43 h  F*D% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *+' +9988 h ' ."MFBPCR"\ATV.dial AIV F*s I +43 h I ."MFBPCR"\AS.seek 0.02ml F*?' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*d'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*v% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*w % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*W% h %Z ."MFBPCR"\AS.seek 0.81ml F*['> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*M'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*#'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *.'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*L'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*`'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*K% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *C'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*, n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *h'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +107 h % ."MFBPCR"\AS.seek 0ml F*1'V +2548 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990315 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )b h  C' 'S h  P% %@ h  F* *o h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *E  h  .t\SPE.configure SPEconfig F*\ 2 *a% h %V .t\AS.configure ASconfig S)5% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'c& S)#.0X( .t\CC.home.seek :open C'$x2 ^22b 'u( +222 h ( +3 ( +161 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 14:00:15 began in tube 4: coreNEG\\n/home/esp/esp2/mission/18tundeployv2.rb:130\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:29:00CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #18) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'8& h & .tHand.open M&{1 +150 h 1 M&& h &M M&3% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&j& h &- M&D% h %η .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +264 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'v(W +416 h (W C'?& h &W .tHand.open M&d1 +150 h 1 M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'y& h &W C'% h %4 .t\CSV.dial CSR C'J  +150 h  C'W% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'i'[ +463 h '[ .t\CSV.dial :waste C'[ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'O'4 +2009 h '4 #t\Purging external Intake C'"& h &W C'M% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# '!  h +75 .t\CTV.dial :intake C'.  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'}'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'g  +75 h #t\Flushing upper manifold C' & h &W C'9% h %4 .t\CSV.dial :flush C']  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +37 h @ C'A% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'L& h &W C'P% h %4 .t\CSV.dial :flush C'K  +38 h  .t\CS.seek 3ml C'`'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'2% h %0 .t\CS.seek 5ml C'C'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'N'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\\'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Q(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C',( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &n(- +264 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &1'η +91 h 'η .tHand.open M&1 +150 h 1 M&/% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:29:06CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 7, 4 => 15, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:29:00CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F* % #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'\ & #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&b% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(S% h %X A(@. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (o(,9 +6 F*E% h %&K ."readyMFB"\ATV.dial :waste2 F*a % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *5' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (c& h &,9 M&Y& h &- M&e% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&6& h &w A(V& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(R& h &,9 .t\SE.seek 13.71 Pucks below Top Plate A(l'n +125 h 'n A(&& h &,9 M&=& h &w .t\Elbow.seek 4 M&X(w +7 h (w M&& h &w A(I& h &,9 .t\Forearm.seek 4 + 300 counts M&H'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&k& A('X h &w M&q% h %7 .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.seek CC M&((ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'x(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'z& h &W ."airStroke"\CSV.dial :waste S)*% ."airStroke"\CS.seek 0ml C'8'4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'9 h 9Td +100 C'p9 h 9kd +100 C'u9 h 9d +100 C'?9 h 9d +100 C'd9 h 9d +100 C'D9 h 9d +100 C'39 h 9d +100 C'y9 h 9d +100 C'9 h 9 d +100 C'J9 h 9 d +100 C'+9 h 96d +100 C' 9 h 9Ld +100 C' 9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'[9 h 9d +100 C'W9 h 9d +100 C'i9 h 9d +100 C'M9 h 9d +100 C'9 h 9d +100 C'!9 h 9d +100 C'"9 h 9(d +100 C'.9 h 9>d +100 C'f9 h 9Td +100 C'#9 h 9id +100 C'O9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'99 h 9d +100 C'}9 h 9d +100 C''9 h 9d +100 C'g9 h 9  d +100 C'A9 h 9 d +100 C'9 h 9 *d +100 C'Z9 h 9 ?d +100 C']9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'r9 h 9 (d +100 C'L9 h 9 ,d +100 C'P9 h 9 0d +100 C'9 h 9 4d +100 C'U9 h 9 8d +100 C'29 h 9  22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:30:45CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST19-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1815h.pcr F*b% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F*1 % +86 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *|'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*$% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +32 h  ."MFBPCR"\ATV.dial :air3 F*t % +87 h % F*A% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*k% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*,'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*z% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1817h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*y'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*=  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*T  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*~% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*8  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*u  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F**'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*F  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*+ n +22 h n ."MFBPCR"delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*9  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1817h.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*l % +87 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*P'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*L% h %n ."MFBPCR"\AS.seek 0.82ml F*0'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*4% h % ."MFBPCR"\AS.seek 0.81ml F*['> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +21 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +21 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1820h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*t  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +22 h ."MFBPCR"delay 2 seconds +200 F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*k n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*/ I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.53ml F*%' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*z % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*:% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*a  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1820h.pcr F*y% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*H% h %Z ."MFBPCR"\AS.seek 0.81ml F*T'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +21 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*8'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +21 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*i  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*F% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*f  +31 h  ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*W% h % ."MFBPCR"\AS.seek 0.81ml F*''> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*l% h %m ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1822h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*_  +42 h  ."MFBPCR"\ARV.dial :carrier F*0  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *]'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*2 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*4% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*b n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*[' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*1  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*S'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*| n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*c'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\ % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*-'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CEST1539901794.97 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1822h.pcr F*$% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*n  +22 h ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *t'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*N% h %Z ."MFBPCR"\AS.seek 0.81ml F*^'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +22 h % ."MFBPCR"delay 3 seconds +300 F*<% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*6% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +64 h  F*%% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +86 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*#% h % ."MFBPCR"\AS.seek 0.81ml F*X'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1900h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ARV.dial :carrier F*u  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*r n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +21 h ."MFBPCR"delay 2 seconds +200 F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*" I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*W% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*M% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.6ml F*.'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*`  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*P  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*_'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*0  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1900h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*{ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *3'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *m' +423 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*h  +43 h  F*K% h % ."MFBPCR"\AS.seek 0.62ml F*2'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*4 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *b'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' ."MFBPCR"\ATV.dial :air3 F*[% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*S% h % ."MFBPCR"\AS.seek 0.71ml F*|' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h % H ."MFBPCR"\AS.seek 0.735ml F* ' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +21 h F*c% h % Ű ."MFBPCR"\AS.seek 0.72ml F*Y' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *7'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*$ % +43 h % F*-% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*e' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +21 h % ."MFBPCR"delay 3 seconds +300 F*N% h %  ."MFBPCR"\AS.seek 0.604ml F*t'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +21 h F*<% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*^' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*/ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*6  +22 h  ."MFBPCR"\AS.seek 0ml F*\\'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*% % +108 h % F*B% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*p% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +21 h F*Z% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*a' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*#  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*X  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *@' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *q'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*; n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *='R +202 h 'R ."MFBPCR"\SPE.coast F*D  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*H'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*V n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*~  +42 h  F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *T' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*5 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*i  +42 h  F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *u': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b **'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*r n +65 h n F*+% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*f I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *W': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *&'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*? n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *l' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*' I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*M  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *!'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*x n +150 h n ."MFBPCR"\AS.seek 0ml F*`'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*. % +43 h % F*L% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.96ml F*0'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*_' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*3'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *K'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *4'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *]'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*b  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *1' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *U' +9988 h ' ."MFBPCR"\ATV.dial AIV F*[ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*Q' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*S'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*| % +43 h % F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*-% h %n ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +86 h % F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*,'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *@'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *q'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*= % +108 h % ."MFBPCR"\AS.seek 0ml F*V'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +3562720 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )~ h  C' ') h  P% %H h  F* *5 h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ C' 2\ F*\ 9\ P% 0\ .t\SPE.coast F*\ 9\ *\ 9\ *T  h  .t\SPE.configure SPEconfig F*\ 2 *E% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)i% h % .t\SS.configure SSconfig C'F& S)#.0X 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:31:44CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #19) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&_1 +150 h 1 M&& h &M M&3% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +166 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &4'η +91 h 'η .tHand.close M&2/ +150 h / M&b& h &- M&% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +264 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'Q& h &W .tHand.open M&S1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'\ & h &W C's% h %4 .t\CSV.dial CSR C'  +150 h  C'w% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'c'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'Y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '$'4 +2009 h '4 #t\Purging external Intake C'-& h &W C'% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'n .t\CBV.dial CSV C'7  h +75 .t\CTV.dial :intake C'A  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'N'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'e  +75 h #t\Flushing upper manifold C'k& h &W C'<% h %4 .t\CSV.dial :flush C't  +75 h  .t\CS.seek 5ml C'6'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +37 h @ C'\\% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'% +113 h .t\CTV.dial :air .\CS.seek 0ml C'B'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +507 h '0 .t\CSV.dial :waste C': +37 h .t\CS.seek 0ml C''4 +507 h '4 C',& h &W C' % h %4 .t\CSV.dial :flush C'p  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +38 h @ C'% h %0 .t\CS.seek 5ml C'Z'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'# +75 h .t\CS.seek 0ml C'a'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &j(ӷ +7 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'D( +416 h ( M&;& h &ӷ M&=% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +264 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &('η +91 h 'η .tHand.open M&V1 +150 h 1 M&I% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:31:50CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 10, 4 => 12, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:31:44CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*v% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'{& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&~% ."readyMFB"\AFV.select :holdingCoil h %\  F* A()% h %X A(H. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (5(,9 +6 F*T% h %&K ."readyMFB"\ATV.dial :waste2 F*E % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *i' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (F& h &,9 M&8& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b & (w +166 h (w M&u& h &w A(r& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(*& h &,9 .t\SE.seek 10.71 Pucks below Top Plate A(f' +155 h ' A(& h &,9 M&g& h &w .t\Elbow.seek 4 M&(w +7 h (w M&"& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &?/ +150 h / ."downElevator"\SE.seek :down M&9& A('X h &w M&&% h %7 .t\Forearm.seek :retract M&l'\ +91 h '\  .t\Elbow.seek CC M&x(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +78 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &J'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S).+ +7 h + S)`% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +387 h ' +50 .t\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'<9 h 9Td +100 C'A9 h 9kd +100 C't9 h 9d +100 C'N9 h 9d +100 C'\\9 h 9d +100 C'69 h 9d +100 C'%9 h 9d +100 C'/9 h 9d +100 C'B9 h 9 d +100 C'$9 h 9 d +100 C'k9 h 96d +100 C'z9 h 9Ld +100 C',9 h 9bd +100 C' 9 h 9xd +100 C'^9 h 9d +100 C'9 h 9d +100 C'o9 h 9d +100 C'9 h 9d +100 C'p9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'#9 h 9(d +100 C' 9 h 9>d +100 C'Z9 h 9Td +100 C'y9 h 9id +100 C':9 h 9d +100 C'X9 h 9d +100 C'9 h 9d +100 C'@9 h 9d +100 C'|9 h 9d +100 C'9 h 9d +100 C'=9 h 9  d +100 C'O9 h 9 d +100 C'19 h 9 *d +100 C'9 h 9 ?d +100 C'd9 h 9 Td +100 C'V9 h 9 j d +100 C';9 h 9 $d +100 C'j9 h 9 (d +100 C'q9 h 9 ,d +100 C'v9 h 9 0d +100 C'~9 h 9 4d +100 C')9 h 9 8d +100 C'H9 h 9 9 h 9\ ld +100 C'g9 h 9 pd +100 C'"9 h 9 td +100 C'G9 h 9 /xd +100 C'9 h 9 C|d +100 C'?9 h 9 Wd +100 C'(9 h 9 kd +100 C'!9 h 9 d +100 C'9 h 9 d +100 C'99 h 9 d +100 C' 9 h 9 d +100 C'+9 h 9 d +100 C'&9 h 9 d +100 C'9 h 9 d +100 C'M9 h 9 d +100 C'9 h 9 d +100 C'f9 h 9 1d +100 C'9 h 9 Dd +100 C'l9 h 9 Xd +100 C'9 h 9 kd +100 C'9 h 9 ~d +100 C'D9 h 9 d +100 C'`9 h 9 d +100 C'9 h 9 d .t\CH.hold 5:00,85C C'W   P h  +30000  C'59 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'P'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'. @ +113 h @ .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 5ml C'K'p +1372 h 'p .t\CSV.dial CTV C'm  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'L @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'i  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'_ @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'b'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C' +113 h .t\CBV.dial CSV C'}  +18 h C'2% h %4 .t\CS.seek 5ml C'['p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'U  +37 h C'h% h %p .t\CS.seek 7ml C']'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +38 h @ .t\CS.seek 10ml C'4' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C' ` +132 h ` #tLysing Complete C'\ & h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&s% h %\  A( % h %X A(Q. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (S( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&'& h &ӷ M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +143 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &-' +89 h ' A(& h & .t\SE.seek 8.71 Pucks below Top Plate A(R' +175 h ' M&w& h & A(7& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &n' +26 h ' .t\Elbow.hold M&e, +6 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ .t\SE.seek 9.04 Pucks below Top Plate A(a'f +27 h 'f M&<% h % A(A& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&t'B +20 h 'B M&N& h & A(\\& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &6' +23 h ' .t\SE.seek 10.04 Pucks below Top Plate A(%'U +36 h 'U M&/& h & M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +89 h '\  .t\Elbow.seek CC M&k(ӷ +143 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&^/ +150 h / .t\CC.seek :open C'( +416 h ( A(o& h & A(& h & M&p& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &#( +142 h ( M& & h & A(Z& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 3 M&y' +93 h ' .t\Elbow.hold M&:, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &X1 +150 h 1 M&% h % A(@& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&|'B +20 h 'B M&& h & A(=& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &O' +22 h ' A(1% h %U ."downElevator"\SE.seek :down A('X +100 (d% h % M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +62 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'j @ +19 h @ .t\CS.seek 10ml C'q' +6 h ' .t\CSV.dial between air and CTV C'v ` +19 h ` .t\AIV.dial :bypass C'~ { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C')& h & .tHand.open M&H1 +2 h 1 M&T& h & M&E% h %\  .t\Elbow.seek FlushPuck::Garage M&I(- +166 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.seek :garage M&8'η +91 h 'η .tHand.close M&{/ +150 h / M&u& h &- M&r% h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &"' +91 h ' .t\CC.seek :closed C'G(W +416 h (W C'& h &W .tHand.open M&?1 +150 h 1 M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +151 h P%9% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%+  +38 h  .t\PS.seek 0ml P%&' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%M' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'f  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%l  h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%D' +2610 h ' .t\PSV.dial :mixloop P%` +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%W  +38 h  .t\PS.seek 2.5ml P%5' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%.' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +7 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &ӷ .t\Forearm.seek CC M&i' +90 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'K( +416 h ( M&& h &ӷ M&}% h % .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &b(- +265 h (- M&U& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &['η +91 h 'η .tHand.open M&1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C' & h & M&\ % h %\  A(s% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (Q(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&'& h &- M&Y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &x' +89 h ' A(w& h &,9 .t\SE.seek 9.71 Pucks below Top Plate A(7' +166 h ' A(& h &,9 M&J& h &w .t\Elbow.seek 4 M&<(w +7 h (w M&A& h &w A(N& h &,9 .t\Forearm.seek 4 + 300 counts M&\\'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &// +150 h / ."downElevator"\SE.seek :down M&B& A(-'X h &w M&c% h %7 .t\Forearm.seek :retract M&R'\ +91 h '\  .t\Elbow.seek CC M&n(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +67 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &t' +91 h ' .t\CC.seek :closed C'a(W +416 h (W .tHand.open M&61 +150 h 1 M&e% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'$  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'o'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'p'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P% ' +2609 h ' .t\PSV.dial :mixloop P%Z +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%k +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%X  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'@ @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'= @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'1  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'# @ +38 h @ .t\CS.seek 10ml C'd' +2647 h ' .t\CSV.dial CTV C'V  +38 h  .t\CS.seek 0ml C'j'4 +2647 h '4 .t\CBV.dial :waste1 C'v @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'~  +150 h C')& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&H% h %\  A(T% h %X A(E. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (y( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (|& h & M&%& h &ӷ M&O% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &C( +143 h ( M&F& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &S' +89 h ' A(4& h & .t\SE.seek 9.71 Pucks below Top Plate A(:' +166 h ' M&{& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &r' +26 h ' .t\Elbow.hold M&;, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(8- h -+ .t\SE.seek 10.04 Pucks below Top Plate A(>'U +27 h 'U M&g% h % A(I& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&*'B +20 h 'B M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &?' +22 h ' .t\SE.seek 11.04 Pucks below Top Plate A(('D +37 h 'D M&& h & M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +89 h '\  .t\Elbow.seek CC M&+(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'f( +416 h ( A(& h & A(l& h & M&M& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &`( +143 h ( M&D& h & A(W& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &51 +150 h 1 M&% h % A(m& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&L'B +21 h 'B M&_& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &!' +22 h ' A(G% h %D ."downElevator"\SE.seek :down A('X +100 (}% h % M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +52 h 'X ."downElevator"\SE.coast A(5 +38 h '\  .t\SC.coast .\PSV.dial :air A(6 P%h  +113 h  P%P% h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%3 +38 h .t\PRV.dial :air P%2  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'\  +38 h .t\PRV.dial between CBV and air P%s  +19 h  .t\PRV.dial :air P%  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %'' +250 h ' .t\PRV.dial :waste2 P%Y @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %[' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&w& h & M&b% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&]/ +150 h / M&/& h &- M&B% h %η .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W C'7& h &W .tHand.open M&<1 +150 h 1 M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  C'K& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%6 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%e  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%$  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%t' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%Q' +1094 h ' .t\PSV.dial :air P%q  +38 h  .t\PS.seek 0ml P%o' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%.% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Z'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +76 h .tdelay 2 seconds +200 P%k% h %r .t\PS.seek 0.475ml P%X' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%@'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%=  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %1' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%# @ +76 h @ .t\PS.seek 0ml P%p' +2609 h ' .t\PSV.dial :air P%V  +76 h  .t\PS.seek 2.5ml P%d' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%v +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%~' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%)  +75 h  .t\PS.seek 2.5ml P%H' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%T +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%E  +75 h P%|% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %%' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'O & +50 h & .t\AWV.select :waste C' +7 P%F% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'4 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %n' +1313 h ' .t\PSV.dial :air P%{  +37 h  .t\PS.seek 2.5ml P%u' +2610 h ' .t\PSV.dial :PML P%8 +38 h .t\PS.seek 0ml P%^' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'g& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'I& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%9% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%S' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*C I +129 h I F*:% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *r'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%? +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%;' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%" +21 h  F*a% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%l% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%M' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%W  +37 h  .t\PS.seek 0ml P%&' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*+  +22 h  F*5% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%m +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%_' +217 ."MFBPCR"\ATV.dial :AIV F* I +42 h I F*G% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%` +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%}' +2610 h ' .t\PSV.dial PSR P%i +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%L' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +38 h  .t\PS.seek 0ml P%h' +739 h ' ."MFBPCR"delay 5 seconds +500 F*P9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*! n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*y'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%3' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +112 h P%s% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%Y +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%'' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %w'k +2679 h 'k .t\PSV.dial :waste1 P%b  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %J' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*A I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%]  +113 h  .t\PS.seek 2.5ml P%/' +2609 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %U' +5106 h ' .t\PSV.dial :air P%N  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %x' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*z  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *c'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*f I +43 h I F*7% h %Q ."MFBPCR"\AS.seek 0.01ml F*<' +171 h ' .t\PSV.dial :mixloop P%- +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %K' +619 h ' ."MFBPCR"\ATV.dial :waste F*6 n +129 h n ."MFBPCR"\AS.seek :empty F*,'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*e  +86 h  F*$% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*[  +43 h F*t% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *q'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'Q  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'.' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  '  +37 h  +113  .t\CBV.dial :waste1 C'k @ +38 h @ .t\CS.seek 0ml C'Z'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*X% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'=  +38 h  C'@% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*#  +22 h F*1% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*V'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*p n +108 h n F*v% h %& ."MFBPCR"\AS.seek 0.092ml F*d'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C')' +1626 h ' ."connect"\CSV.dial CTV C'~  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'T'4 +2647 h '4 C'E& h &W #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'( +416 h ( M&{& h &ӷ M&n% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&9% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'C& h & M&:% h %\  A( % h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (S(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (*& h &,9 M&& h &- M&?% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &>(w +166 h (w M&& h &w A(a& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &"' +90 h ' A(l& h &,9 ."WCR"\SE.seek 8.71 Pucks below Top Plate A(r' +175 h ' A(;& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M&(w +7 h (w M&W& h &w A(+& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&5'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &M/ +79 h '&0 ."MFBPCR"delay 3 seconds +71 h / ."downElevator"\SE.seek :down A(D'X M&m& h &w M&% h %7 ."WCR"\Forearm.seek :retract M&G'\ +91 h '\  ."WCR"\Elbow.seek CC M&&(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +54 ."MFBPCR"\ATV.dial :waste2 F* % +4 h (ӷ M&`& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &i' +39 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +52 h ' ."WCR"\CC.seek :closed C'}(W +416 h (W ."WCR"Hand.open M&(1 +91 h 'R ."MFBPCR"\ATV.dial :air2 F*L  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *P' +37 h 1 M&!% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'h& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'2  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +108 h % F*"% h %V ."MFBPCR"\AS.seek 0.59ml F*`' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*r' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*D% h % ."MFBPCR"\AS.seek 0.12ml F*G' +625 h ' ."MFBPCR"\ATV.dial :air3 F*d % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +22 h F*!% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*S% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*s n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*\  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b * '5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*3 @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*y I +85 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*b  +42 h  #"MFBPCR"\Swish #1 of 2 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *A'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*]'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*'% h %(A ."MFBPCR"\AS.seek 0.6ml F*/'\  +1031 h '\  F*N% h %\  ."MFBPCR"\AS.seek 0.5ml F*U'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*z% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *J' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*B % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*f  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1917h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*7  +64 h  ."MFBPCR"\ARV.dial :carrier F*-  +11 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *e'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*[  +21 h ."MFBPCR"delay 2 seconds +200 F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *,'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*j' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*k % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*X  +107 h  ."MFBPCR"delay 7 seconds +700 F*=% h %< ."MFBPCR"\AS.seek 0.594ml F*@'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*1  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.65ml F*p' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*V  +65 h ."MFBPCR"\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*~% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226137 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'O  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'E @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '4' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)F% h % ."WCR"\CBV.dial between CSV and waste2b C'\\  +94 h  C'{% h % ."WCR"\CSV.dial between air and CTV C'R ` +18 h ` ."WCR"\CS.seek 7.288ml C'%' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'g  'n  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'H'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'I' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'8  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'9  +37 h  C':% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'C @ +75 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'?  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'a' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'l  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ';'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'+  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'^  +38 h  C'W% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'M' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'> @ +75 h @ +50 S)"% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'_% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'`' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'5  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'D'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'r  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'd' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'G& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&(% h %\  A(!% h %X A(L. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (h( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (2& h & M&& h &ӷ M&i% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &S( +142 h ( M&& h & A(s& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +89 h ' A(& h & ."WCR"\SE.seek 10.71 Pucks below Top Plate A(' +155 h ' M& & h & A( & h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &y' +26 h ' ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+ ."WCR"\SE.seek 11.04 Pucks below Top Plate A(b'D +27 h 'D M&w% h % A(Y& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&A& h & A('& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &]' +23 h ' ."WCR"\SE.seek 12.04 Pucks below Top Plate A(N'3 +36 h '3 M&/& h & M&z% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  ."WCR"\Elbow.seek CC M&B(ӷ +143 h (ӷ M&J& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ ."WCR"\Forearm.seek CC M&7' +91 h ' ."WCR"Hand.close M&-/ +150 h / ."WCR"\CC.seek :open C'c( +416 h ( A(& h & A(& h & M&P& h &ӷ M&6% h % ."WCR"\Forearm.seek :retract M&e'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &<( +142 h ( M&[& h & A(t& h & ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.seek 3 M&$' +93 h ' ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&,% h % A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&o'B +20 h 'B M&Q& h & A(k& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &.' +22 h ' A(j% h %3 ."downElevator"\SE.seek :down A(X'X +100 (=% h % M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +43 h 'X ."downElevator"\SE.coast A(5 +47 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&@1 +2 h 1 M&1& h & M&#% h %\  .t\Elbow.seek FlushPuck::Garage M&q(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.seek :garage M&v'η +91 h 'η .tHand.close M&~/ +150 h / M&p& h &- M&% h %η .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +264 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'\\& h &W .tHand.open M&{1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'n% h % .t\CSV.dial CSR C'%  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '8% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '9'[ +463 h '[ .t\CSV.dial :waste C': +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'I'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'C% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '?'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  '  +38 h  +113  .t\CBV.dial :waste1 C'a @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C';& h &W C'+% h %4 .t\CSV.dial :flush C'^  +75 h  .t\CS.seek 5ml C'W'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'M% h %p .t\CS.seek 7ml C'>'i +416 h 'i ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'_'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'm% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'5'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'r% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'G  +38 h +75 .t\CTV.dial :air C'(  +151 h .t\CS.seek 0ml C'!'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'L'0 +506 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C''4 +506 h '4 C'i& h &W C'% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C' % h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'w +75 h .t\CS.seek 0ml C'Y'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'A& h &W C''% h %4 .t\CSV.dial CSR C'/  +150 h  C'z% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'\ 'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'S' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'y  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'J' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'f  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'b  +113 h  C']% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '-' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'U  '}  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'P  +38 h  C'6% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '['P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +710 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'B  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C',  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'Q'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'k' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'j 'e  +37 h +75 .t\CTV.dial :air C'<  +151 h .t\CS.seek 0ml C'='4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'Z +38 h .t\CS.seek 0ml C'@'4 +507 h '4 #t\Flushing upper manifold C'1& h &W C'#% h %4 .t\CSV.dial :flush C'$  +37 h  .t\CS.seek 5ml C'o'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'N @ +38 h @ C'% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'. +113 h .t\CTV.dial :air .\CS.seek 0ml C'X'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'h'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'~'4 +507 h '4 C'p& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'v'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C'T% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'|'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'c'0 +506 h '0 .t\CSV.dial :waste C'\\ +76 h .t\CS.seek 0ml C'{'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'R& h &W C'g% h %4 .t\CSV.dial :flush C'n  +38 h  .t\CS.seek 5ml C'%'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'8  'H  +37 h  +113  .t\CBV.dial :waste1 C': @ +38 h @ .t\CS.seek 0ml C'9'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'C% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'I'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'l  +75 h  .t\CS.seek 0ml C'a'4 +766 h '4 #tDisinfecting external Intake with kill C'*& h &W C';% h %4 .t\CSV.dial CSR C'+  +38 h  C'^% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'u' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W 'M  +38 h +75 .t\CTV.dial :intake C'"  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'>  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'm  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'_'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'`' +1372 h ' .t\CSV.dial CBV C'r +113 h .t\CS.seek 0ml C'5'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.seek CC M&!' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C'L( +416 h ( M&i& h &ӷ M&% h % .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&A1 +2 h 1 M&'& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &/(- +6 h (- M&z& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.seek :garage M&\ 'η +91 h 'η .tHand.close M&/ +150 h / M&y& h &- M&S% h %η .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &J(ӷ +265 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +415 h (W C'U& h &W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'P& h &W C'6% h %4 .t\CSV.dial CSR C'  +113 h  C'[% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'B% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +463 h '[ .t\CSV.dial :waste C', +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'& h &W C'Q% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'< +75 h .t\CTV.dial :intake C'k  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'='4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'Z  +75 h #t\Flushing upper manifold C' & h &W C'1% h %4 .t\CSV.dial :flush C'#  +75 h  .t\CS.seek 5ml C'$'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'N% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'o +113 h .t\CTV.dial :air .\CS.seek 0ml C'.'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'X +37 h .t\CS.seek 0ml C''4 +507 h '4 C'h& h &W C'p% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +38 h @ C'E% h %0 .t\CS.seek 5ml C'T'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'v'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +506 h '0 .t\CSV.dial :waste C'| +76 h .t\CS.seek 0ml C'\\'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +7 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&%& h &ӷ M&8% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M& 1 +150 h 1 M&9% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST20-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:33:32CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST20-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1917h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*l % +86 h % F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*+% h %Z ."MFBPCR"\AS.seek 0.81ml F*^'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*M'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*u'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*>  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*2 % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *4' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*i% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*A' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct1919h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*b  +11 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*/'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*P  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*[  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*5' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*B  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*,' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*t  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*e  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*<'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*k n +22 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*Z'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*@  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*N% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct1919h1500ml.pcr F*.% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*V  +21 h ."MFBPCR"\ATV.dial :air3 F*h % +87 h % F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*~% h %Z ."MFBPCR"\AS.seek 0.81ml F*E'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*q% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *J'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*-' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct1921h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*u  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*_'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*` n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +22 h ."MFBPCR"delay 2 seconds +200 F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *F'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*i n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*4% h %Fj ."MFBPCR"\AS.seek 0.524ml F*!' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*& I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*s'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*' n +22 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*b'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct1921h1500ml.pcr F*/% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*U % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *P'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*}% h %Z ."MFBPCR"\AS.seek 0.81ml F*['> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*B'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +21 h F*5% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*,  +65 h  F*e% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +31 h  ."MFBPCR"\ATV.dial :air3 F*= % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*o% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +21 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*$'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +21 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*X  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*~' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V @CEST1539986419.60 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2000h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*c  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*-'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*% n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *8'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*H I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*C% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*l  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*;  +108 h  ."MFBPCR"delay 7 seconds +700 F*I% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +21 h n ."MFBPCR"delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*^  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*M  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*>  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*u'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2000h1500ml.pcr F*m% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*`  +22 h ."MFBPCR"\ATV.dial :air3 F*r % +86 h % F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*D'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*F'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*!'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*b'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*U% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*L  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2002h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ARV.dial :carrier F*t  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*Q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +21 h ."MFBPCR"delay 2 seconds +200 F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *1'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*# I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*3 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*V  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*X  +22 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*|' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*v  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*g% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*J % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2002h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*n @@T *-  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :ASV F*R n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*{% h % ."MFBPCR"\AS.seek 0.62ml F*H'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*C n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*)'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*9 % +43 h % F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *?' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*;' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F**' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % H ."MFBPCR"\AS.seek 0.735ml F*^' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*M' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*> n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*"' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*m % +43 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*G% h % ."MFBPCR"\AS.seek 0.579ml F*_' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +21 h % ."MFBPCR"delay 3 seconds +300 F*(% h %  ."MFBPCR"\AS.seek 0.604ml F*2'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +21 h F*4% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*D' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*& n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*F'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*!% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*s% h % ."MFBPCR"\AS.seek 0.58ml F*'' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +21 h % ."MFBPCR"delay 3 seconds +300 F*A% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*S% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*]  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *f' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*U n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *b'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*L n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*6  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*P n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *[' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +42 h  F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *t': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*,% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *Q'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*j n +65 h n F*k% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Z I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *o': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*1  +64 h  F*3% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *.'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*N n +65 h n F*V% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *h' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *X'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*p  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *$': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*~ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*T'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*E  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *v' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*|% h % ."MFBPCR"\AS.seek 0.96ml F*c'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*-% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*8% h % ."MFBPCR"\AS.seek 0.97ml F*%'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*R I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *{'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *C'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*9% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *)' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*?' +9880 h ' ."MFBPCR"\ATV.dial :waste F*I n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*;% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*+  +21 h ."MFBPCR"\ATV.dial :air3 F** % +43 h % F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*u% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*r  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +64 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +86 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +22 h F*A% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *b'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*P'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990367 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% %} h  F* *7 h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *[  h  .t\SPE.configure SPEconfig F*\ 2 *5% h %V .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'e& S)#.0X 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:34:31CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #20) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'8& h & .tHand.open M&1 +150 h 1 M&R& h &M M&%% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &C'η +91 h 'η .tHand.close M&H/ +150 h / M&& h &- M&9% h %η .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &l(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &I' +91 h ' .t\CC.seek :closed C';(W +416 h (W C'?& h &W .tHand.open M&1 +150 h 1 M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'W& h &W C'a% h %4 .t\CSV.dial CSR C'^  +150 h  C'>% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'M% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'm'4 +2009 h '4 #t\Purging external Intake C'u& h &W C'"% h %4 .t\CSV.dial :flush C'r  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'G' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'`  ' h +75 .t\CTV.dial :intake C'O  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'('4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'_  +75 h #t\Flushing upper manifold C'i& h &W C'4% h %4 .t\CSV.dial :flush C'2  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +37 h @ C'F% h %p .t\CS.seek 7ml C'&'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'!'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C's'4 +507 h '4 C' & h &W C'z% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'Y @ +38 h @ C''% h %0 .t\CS.seek 5ml C'S'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C'y +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +7 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.seek CC M&U' +91 h ' .tHand.close M&f/ +150 h / .t\CC.seek :open C'6( +416 h ( M&L& h &ӷ M&% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&01 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:34:37CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 13, 4 => 9, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:34:31CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C'n& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(}. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (7(,9 +6 F*[% h %&K ."readyMFB"\ATV.dial :waste2 F*5 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (e& h &,9 M&B& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &,(w +166 h (w M&t& h &w A(j& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &k' +89 h ' A(Q& h &,9 .t\SE.seek 7.71 Pucks below Top Plate A(Z' +185 h ' A(<& h &,9 M&=& h &w .t\Elbow.seek 4 M& (w +6 h (w M&#& h &w A(@& h &,9 .t\Forearm.seek 4 + 300 counts M&3'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &N/ +150 h / ."downElevator"\SE.seek :down A(V'X M&1& h &w M&.% h %7 .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'-(W +416 h (W .tHand.open M&J1 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +6 h + S)T% h % C'8& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +1336 h ' ."readyMFB"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."readyMFB"delay 1 second +100 .\ATV.dial :waste F*I n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *X'R +5065 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*M  h  ."readyMFB"\ASV.dial :waste1 F*  h +1536 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'% h %4 S)% h % S)u% h % .tIntake.select :close S)0 +6 )"% h % .t\SSV.dial :exhaust S)r  +75 h  .t\Exhaust.select :open S)@ +7 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9xd +100 C'`9 h 9|d +100 C'9 h 9d +100 C'_9 h 9d +100 C'G9 h 9'd +100 C'9 h 9>d +100 C'49 h 9Td +100 C'O9 h 9kd +100 C'29 h 9d +100 C'(9 h 9d +100 C'F9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'&9 h 9d +100 C'!9 h 9 d +100 C'm9 h 9 d +100 C'i9 h 96d +100 C' 9 h 9Ld +100 C' 9 h 9bd +100 C'z9 h 9xd +100 C'D9 h 9d +100 C's9 h 9d +100 C'Y9 h 9d +100 C''9 h 9d +100 C'A9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'y9 h 9(d +100 C'9 h 9>d +100 C'S9 h 9Td +100 C'/9 h 9id +100 C'9 h 9d +100 C']9 h 9d +100 C'\ 9 h 9d +100 C'f9 h 9d +100 C'*9 h 9d +100 C';9 h 9d +100 C'9 h 9  d +100 C'U9 h 9 d +100 C'l9 h 9 *d +100 C'K9 h 9 ?d +100 C'\\9 h 9 Td +100 C'09 h 9 j d +100 C'L9 h 9 $d +100 C'w9 h 9 (d +100 C'b9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'}9 h 9 & h & A(`& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&_, +6 h , .t\Elbow.reconfigure TightElbow A(G- M&$&.,(\ #b h -+Ɛ .t\SE.seek 12.04 Pucks below Top Plate A('3 +27 h '3 M&4% h % A(O& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&2'B +20 h 'B M&(& h & A(F& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +23 h ' .t\SE.seek 13.04 Pucks below Top Plate A('" +36 h '" M&&& h & M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +89 h '\  .t\Elbow.seek CC M&i(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&z' +91 h ' .tHand.close M&D/ +150 h / .t\CC.seek :open C's( +416 h ( A(Y& h & A('& h & M&A& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &y( +142 h ( M&& h & A(S& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 3 M&/' +93 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &]1 +150 h 1 M&\ % h % A(f& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&*'B +20 h 'B M&;& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &U' +22 h ' A(l% h %" ."downElevator"\SE.seek :down A(K'X +100 (\\% h % M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +34 h 'X ."downElevator"\SE.coast A(5 +56 h '\  .t\SC.coast A(6 #tTranferring lysate to PRV .\CSV.dial :air C'w @ +19 h @ .t\CS.seek 10ml C'b' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&}1 +2 h 1 M&[& h & M&5% h %\  .t\Elbow.seek FlushPuck::Garage M&x(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&n/ +150 h / M&t& h &- M&j% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &#' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'<& h &W .tHand.open M&N1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%@ +151 h P%1% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%,' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%k  +38 h  .t\PS.seek 0ml P%.' +1597 h ' .t\PSV.dial PSR P%g +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%p' +2610 h ' ."connect"\PRV.dial CBV P%3  .t\CBV.dial PRV C'Z  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%h  h .t\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial :air P%J  +37 h  .t\PS.seek 2.5ml P%6' +2610 h ' .t\PSV.dial :mixloop P%T +38 h .t\PS.seek 0ml P%8' +2609 h ' .t\PSV.dial :air P%o  +38 h  .t\PS.seek 2.5ml P%~' +2610 h ' .t\PSV.dial :mixloop P%% +37 h .t\PS.seek 0ml P% ' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'{( +416 h ( M&9& h &ӷ M&|% h % .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&c1 +150 h 1 M&R% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'+& h & M&W% h %\  A(a% h %X A(^. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (?(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (I& h &,9 M&& h &- M&M% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A("& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(>& h &,9 .t\SE.seek 6.71 Pucks below Top Plate A(`' +196 h ' A(G& h &,9 M&4& h &w .t\Elbow.seek 4 M&O(w +6 h (w M&$& h &w A((& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +23 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &&/ +150 h / ."downElevator"\SE.seek :down A(!'X M&u& h &w M&% h %7 .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +104 h 'X ."downElevator"\SE.coast A(5 +38 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M&_% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'm  +150 h  .t\CSV.dial CTV C'-  +19 h  .t\CS.seek 5ml C'Y'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C''' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'A'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%S +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%z  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%i +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%]  +151 h  .t\PS.seek :empty P%\ ' +2609 h ' .t\CBV.dial :waste1 C'f @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C';  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'l  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'y @ +37 h @ .t\CS.seek 10ml C'\\' +2648 h ' .t\CSV.dial CTV C'0  +37 h  .t\CS.seek 0ml C'w'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&}% h %\  A([% h %X A(5. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (/( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (*& h & M&& h &ӷ M&U% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &E( +143 h ( M&e& h & A(K& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(C& h & .t\SE.seek 12.71 Pucks below Top Plate A(' +136 h ' M&n& h & A(t& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &j' +26 h ' .t\Elbow.hold M&L, +6 h , .t\Elbow.reconfigure TightElbow A(B- M&$&.,(\ #b h -+ .t\SE.seek 13.04 Pucks below Top Plate A(:'" +27 h '" M&=% h % A(x& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&Q'B +20 h 'B M&s& h & A(<& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &N' +22 h ' .t\SE.seek 14.04 Pucks below Top Plate A(' +37 h ' M&@& h & M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &#'\ +89 h '\  .t\Elbow.seek CC M&k(ӷ +143 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M&.' +90 h ' .tHand.close M&3/ +150 h / .t\CC.seek :open C'Z( +416 h ( A( & h & A(h& h & M&p& h &ӷ M&J% h % .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &T( +143 h ( M&6& h & A(o& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &8' +92 h ' .t\Elbow.hold M&%, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &~1 +150 h 1 M&7% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&q'B +21 h 'B M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(P% h % ."downElevator"\SE.seek :down A(9'X +100 (|% h %v M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +22 h 'X ."downElevator"\SE.coast A(5 +68 h '\  .t\SC.coast .\PSV.dial :air A(6 P%  +113 h  P%v% h % .t\PS.seek 0.499ml P%c' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%R +38 h .t\PRV.dial :air P%H  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%+  +37 h .t\CBV.dial AIV C'W  +38 h .t\PRV.dial between CBV and air P%a  +19 h  .t\PRV.dial :air P%^  +19 h  P%I% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%M @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&"1 +2 h 1 M&>& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&G(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M&d/ +150 h / M&&& h &- M&u% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'`& h &W .tHand.open M&O1 +150 h 1 M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  C'{& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%_  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%m  +75 h  .tdelay 2 seconds +200 P%-% h % .t\PS.seek 1.499ml P%2' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%?' +1094 h ' .t\PSV.dial :air P%b  +38 h  .t\PS.seek 0ml P%Y' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%' +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %S'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%z  +76 h .tdelay 2 seconds +200 P%i% h %r .t\PS.seek 0.475ml P%]' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%f'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%;  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %l' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%y @ +76 h @ .t\PS.seek 0ml P%A' +2609 h ' .t\PSV.dial :air P%0  +76 h  .t\PS.seek 2.5ml P%\\' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%}' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%[ +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h P%*% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'U & +50 h & .t\AWV.select :waste C' +7 P%e% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %K'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'C { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%n  +37 h  .t\PS.seek 2.5ml P%t' +2610 h ' .t\PSV.dial :PML P%B +38 h .t\PS.seek 0ml P%=' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'x& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'D& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*s @@T .t\AWV.select :air F*<  .t\AIV.dial :bypass .\PSV.dial PSR P%@ +21 h  ."MFBPCR"delay 20 seconds +92 h P%1% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*E I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *j'6 +66 .t\PSV.dial :waste1 P%,  +38 h  .t\PS.seek 0ml P%g' +1597 h ' .t\PSV.dial PSR P%Q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%:' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%N +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%L' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*3  +22 h ' .t\PSV.dial PSR P%# +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%h% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%p' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*J % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%o  +37 h  .t\PS.seek 0ml P%.' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*k  +22 h  F*~% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F* I +42 h I F*P% h % ."MFBPCR"\AS.seek 0.469ml F*V' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%T +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%|' +2610 h ' .t\PSV.dial PSR P% +113 h P%8% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%q' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +739 h ' ."MFBPCR"delay 5 seconds +500 F*v9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*9% h % ."MFBPCR"\AS.seek 0.041ml F*/'% +336 h ' .t\PSV.dial PSR P%\ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%R' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%+' +2610 h ' .t\PSV.dial PSR P%W +112 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%I  +38 h  .t\PS.seek 0ml P%c' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%M +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%" +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %>'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %4' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*$ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *%'V +850 h ' .t\PSV.dial :air P%d  +113 h  .t\PS.seek 2.5ml P%&' +2609 h ' .t\PSV.dial :mixloop P%u +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %)' +5106 h ' .t\PSV.dial :air P%(  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*`% h %Q ."MFBPCR"\AS.seek 0.01ml F*O' +171 h ' .t\PSV.dial :mixloop P%! +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %{' +619 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*_  +86 h  F*m% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *-' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*2% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *b'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'?  +38 h  C''% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Y  'z  +37 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C'S'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*]% h %_ ."MFBPCR"\AS.seek 0.434ml F*;'ݏ +110 h '4 .t\CSV.dial CSR C'  +38 h  C'f% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*y  +22 h F*l% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*0'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*A n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*\\'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'['4 +2647 h '4 C'5& h &W #tGaraging FlushPuck in Collection Clamp M&*& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +7 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&C/ +150 h / .t\CC.seek :open C'K( +416 h ( M&n& h &ӷ M&% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &t(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &<'η +91 h 'η .tHand.open M&@1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'E& h & M&% h %\  A(,% h %X A(X. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Q& h &,9 M&g& h &- M&N% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &:(w +166 h (w M&3& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &#' +90 h ' A(h& h &,9 ."WCR"\SE.seek 5.71 Pucks below Top Plate A(j' +205 h ' A(L& h &,9 M&J& h &w ."WCR"\Elbow.seek 4 M& (w +6 h (w M&o& h &w A(k& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&~'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &p/ +50 h '&0 ."MFBPCR"delay 3 seconds +100 h / ."downElevator"\SE.seek :down M&& A(6'X h &w M&% h %7 ."WCR"\Forearm.seek :retract M&P'\ +91 h '\  ."WCR"\Elbow.seek CC M&.(ӷ +109 ."MFBPCR"\ATV.dial :waste2 F*7 % +5 h 'X ."downElevator"\SE.coast A(5 +29 h (ӷ M&T& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +9 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +82 h ' ."WCR"\CC.seek :closed C'|(W +416 h (W ."WCR"Hand.open M&1 +61 h 'R ."MFBPCR"\ATV.dial :air2 F*q  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *v' +67 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)V+ +6 h + S)\ % h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'H  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %W S)$% h %W ."WCR"Intake.select :close S)0 +6 )% h %W ."WCR"\SSV.dial :exhaust S)d  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *$'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*d'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*&'\  +1031 h '\  F*(% h %\  ."MFBPCR"\AS.seek 0.5ml F*)'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F* % h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *4' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*u % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +3472 h 'V ."MFBPCR"\SPE.coast F*Z  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2017h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*`  +64 h  ."MFBPCR"\ARV.dial :carrier F*!  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*v n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*-  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*i % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.65ml F*A' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*;  +43 h  ."MFBPCR"\ASV.dial :waste1 F*0  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F** % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226166 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'U  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'5 @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)e% h % ."WCR"\CBV.dial between CSV and waste2b C'F  +94 h  C'n% h % ."WCR"\CSV.dial between air and CTV C'r ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'x  ."WCR"\CSV.dial CTV C'  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '}'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'@  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'D' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'B  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'1  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'X' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'E @ +76 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'N  +94 h  C'<% h % ."WCR"\CSV.dial between air and CTV C'g ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'h  '3  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'L'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'J  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 't' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'o  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'k  +37 h  C'=% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'p' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C': @ +75 h @ +50 S)#% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'7 ` +56 h ` ."WCR"\CS.seek 7.288ml C'T' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'j  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '6'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'~  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '\\' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'K  +151 h C'P& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(% h %X A(q. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( ( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (8& h & M&\ & h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&H& h & A(a& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &W' +89 h ' A(V& h & ."WCR"\SE.seek 13.71 Pucks below Top Plate A(I'n +125 h 'n M&+& h & A(^& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &/' +26 h ' ."WCR"\Elbow.hold M&c, +6 h , ."WCR"\Elbow.reconfigure TightElbow A("- h -+n M&$&.,(\ #b ."WCR"\SE.seek 14.04 Pucks below Top Plate A(' +27 h ' M&>% h % A(M& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&$& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +23 h ' ."WCR"\SE.seek 15.04 Pucks below Top Plate A((' +36 h ' M&&& h & M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +89 h '\  ."WCR"\Elbow.seek CC M&u(ӷ +143 h (ӷ M&4& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ ."WCR"\Forearm.seek CC M&`' +91 h ' ."WCR"Hand.close M&!/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(%& h & A(G& h & M&v& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&_'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &O( +142 h ( M&& h & A(2& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &m' +93 h ' ."WCR"\Elbow.hold M&-, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &'1 +150 h 1 M& % h % A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&Y'B +20 h 'B M&?& h & A(i& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(w% h % ."downElevator"\SE.seek :down A(]'X +100 (% h %- M&z% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +13 h 'X ."downElevator"\SE.coast A(5 +77 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&f1 +2 h 1 M&l& h & M&y% h %\  .t\Elbow.seek FlushPuck::Garage M&b(- +166 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&A& h &- M&% h %η .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +264 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'e(W +416 h (W C'F& h &W .tHand.open M&n1 +150 h 1 M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'x% h % .t\CSV.dial CSR C'  +38 h  C'@% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'}'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'B% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '1'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'X& h &W C'E% h %4 .t\CSV.dial :flush C',  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'N'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'g% h %p .t\CS.seek 7ml C'Q'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'3  'h  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'J'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'L& h &W C'o% h %4 .t\CSV.dial :flush C'k  +75 h  .t\CS.seek 5ml C'='p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'p% h %p .t\CS.seek 7ml C':'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'#  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'7& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'j'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +37 h @ C'~% h %p .t\CS.seek 7ml C'6'i +416 h 'i ."connect"\CSV.dial CBV C'K .t\CBV.dial CSV C'P  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +506 h '0 .t\CSV.dial :waste C'8 +38 h .t\CS.seek 0ml C'\ '4 +506 h '4 C'& h &W C'H% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 3ml C'V'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +37 h @ C'+% h %0 .t\CS.seek 5ml C'^'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'"'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'>'0 +507 h '0 .t\CSV.dial :waste C'M +75 h .t\CS.seek 0ml C'$'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'9% h %4 .t\CSV.dial CSR C'&  +150 h  C' % h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'W'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I +113 h .t\CTV.dial :intake C'/  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'4' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Z  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'd% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'c'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +710 h ' ."connect"\CSV.dial CTV C')  .t\CTV.dial CSV C'|  +38 h  +113  .t\CBV.dial :waste1 C'% @ +37 h @ .t\CS.seek 0ml C'G'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'v  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  +38 h  .t\CBV.dial :waste2b C'u  +75 h  .t\CS.seek 0ml C'''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '?'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w '_  +37 h +75 .t\CTV.dial :air C'O  +151 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C'f'4 +507 h '4 #t\Flushing upper manifold C'l& h &W C'y% h %4 .t\CSV.dial :flush C'm  +37 h  .t\CS.seek 5ml C'Y'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +38 h @ C';% h %p .t\CS.seek 7ml C'0'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C']'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'- +37 h .t\CS.seek 0ml C''4 +507 h '4 C'A& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'5 @ +38 h @ C'[% h %0 .t\CS.seek 5ml C'b'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'*'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'F +76 h .t\CS.seek 0ml C'n'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'r& h &W C'% h %4 .t\CSV.dial :flush C'x  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CTV C'B  .t\CTV.dial CSV C'}  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'1'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'X& h &W C'E% h %4 .t\CSV.dial :flush C',  +75 h  .t\CS.seek 5ml C'D'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'g% h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'3  +38 h  .t\CBV.dial :waste2b C'h  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'Q& h &W C'L% h %4 .t\CSV.dial CSR C'o  +38 h  C'k% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'J'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C't' +416 h ' ."connect"\CSV.dial CBV C'p .t\CBV.dial CSV C'=  +38 h +75 .t\CTV.dial :intake C'#  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C':  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '7'4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'T' +1372 h ' .t\CSV.dial CBV C'~ +113 h .t\CS.seek 0ml C'j'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&P& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &K(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&8/ +150 h / .t\CC.seek :open C'q( +416 h ( M&& h &ӷ M&H% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &\ (- +264 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &V'η +91 h 'η .tHand.open M&^1 +150 h 1 M&"% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'>& h & .tHand.open M&1 +2 h 1 M&9& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +6 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.seek :garage M&W'η +91 h 'η .tHand.close M&I/ +150 h / M&/& h &- M&% h %η .t\Forearm.seek :retract M&Z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'c(W +415 h (W C')& h &W .tHand.open M&|1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'v& h &W C'% h %4 .t\CSV.dial CSR C'G  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'`'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'u% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '2'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C''& h &W C'?% h %4 .t\CSV.dial :flush C'_  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +75 h .t\CTV.dial :intake C'i  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'S  +75 h #t\Flushing upper manifold C'z& h &W C'l% h %4 .t\CSV.dial :flush C'y  +75 h  .t\CS.seek 5ml C'm'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +37 h @ C'(% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CBV C'Y .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +507 h '0 .t\CSV.dial :waste C'] +37 h .t\CS.seek 0ml C'-'4 +507 h '4 C' & h &W C'A% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'5% h %0 .t\CS.seek 5ml C'['p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +506 h '0 .t\CSV.dial :waste C'* +76 h .t\CS.seek 0ml C'F'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&B% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &E'η +91 h 'η .tHand.open M&,1 +150 h 1 M&1% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST21-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:36:20CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST21-Oct-18" +263689 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2017h1500ml.pcr F*g% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*3  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +21 h F*o% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*='n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*#% h %n ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F*8 % +87 h % F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*H'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*+'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*"'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *>'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2019h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*&'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*%  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*v  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*j' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*u  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*`% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*2  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*?'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*_  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*O'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +22 h n ."MFBPCR"delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*z  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*f  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*(% h % \\ ."MFBPCR"\AS.seek 0.326ml F*m'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Y  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*; % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2019h1500ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*0  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*5'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*b% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*X  +32 h  ."MFBPCR"\ATV.dial :air3 F*r % +86 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *1' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*E% h % ."MFBPCR"\AS.seek 0.81ml F*s'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*N% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*L  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2021h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*t  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*7% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*T n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *P'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*8  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *e'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*C% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*. I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.53ml F*K' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*^ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*a'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +107 h  ."MFBPCR"delay 7 seconds +700 F*>% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*9 n +22 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*$'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*M % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2021h1500ml.pcr F*&% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*) % +87 h % F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *v'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*u'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +21 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*'  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*Y% h % ."MFBPCR"\AS.seek 0.81ml F*;'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*m'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*]  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V @CEST1540072819.60 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2100h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F*!'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*X  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +22 h ."MFBPCR"delay 2 seconds +200 F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*} I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*1' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*g n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*s' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*h  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.536ml F*3'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*L  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Q n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*k  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.65ml F*#' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*=  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*:  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*t'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2100h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*6'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*e'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +64 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *"'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*I  +32 h  ."MFBPCR"\ATV.dial :air3 F*$ % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\ ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*)% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2102h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*w n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *l'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*y I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*Y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*(' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*R % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*;' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*0  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*m'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +22 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*-'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*[  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F**' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*b  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*@'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*4 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2102h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*x @@T ."MFBPCR"\ASV.dial :SPEdelivery F*!  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*c  +151 h F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *X' +423 h ' ."MFBPCR"\ATV.dial :ASV F*r n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*,  +43 h  F*n% h % ."MFBPCR"\AS.seek 0.62ml F*}'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*E n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\\'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *g'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*1 % +43 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *N' +424 h ' ."MFBPCR"\ATV.dial :air3 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F*3% h % ."MFBPCR"\AS.seek 0.71ml F*Q' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h % H ."MFBPCR"\AS.seek 0.735ml F*k' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +21 h F*J% h % Ű ."MFBPCR"\AS.seek 0.72ml F*=' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*#' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *7'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*t% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*P% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*8'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*C% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*6' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :wasteR F*H  +22 h  ."MFBPCR"\AS.seek 0ml F*e'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*K % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*a% h % ."MFBPCR"\AS.seek 0.58ml F*9' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*I'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*V' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*W n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*/  +21 h  ."MFBPCR"\AS.seek 0ml F*\ 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *&'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*d  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *Z' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*) n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*q n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *%'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*|'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*v n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*` I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*'  +42 h  F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *2': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F* % h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *?'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*w n +65 h n F*i% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *O' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*S I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*z  +43 h  F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Y': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*l  +64 h  F*R% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*( n +65 h n F*0% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*; I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*A  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *m': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*- n +150 h n ."MFBPCR"\AS.seek 0ml F*['V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*5  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F**% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %0 ."MFBPCR"\AS.seek 0.985ml F*@' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*B% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*r I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *n'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *E'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\\'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*g  +43 h  F*1% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*s I +43 h I ."MFBPCR"\AS.seek 0.02ml F*N' +9880 h ' ."MFBPCR"\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.seek 0ml F*3'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*L% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*o  +21 h ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *J'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*:% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*#'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*t% h %n ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*~  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*P  +64 h  F*T% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*H  +32 h  ."MFBPCR"\ATV.dial :air3 F*6 % +86 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*$  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*I' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Z'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*% % +108 h % ."MFBPCR"\AS.seek 0ml F*v'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast .\Forearm.coast P%6 M&5 ."shutdown"\Elbow.coast +2 C'5 M&6 P%5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990367 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' 'G h  P% %| h  F* *` h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ P% 0\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *j% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)'% h % .t\SS.configure SSconfig C'_& S)#.0X 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:37:19CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #21) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'B& h & .tHand.open M&@1 +150 h 1 M&r& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &,(- +166 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &E'η +91 h 'η .tHand.close M&}/ +150 h / M&g& h &- M&1% h %η .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +264 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &D' +91 h ' .t\CC.seek :closed C'L(W +416 h (W C'N& h &W .tHand.open M&31 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'p& h &W C'% h %4 .t\CSV.dial CSR C'k  +150 h  C':% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'J'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '=% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C't& h &W C'#% h %4 .t\CSV.dial :flush C'~  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'T .t\CBV.dial CSV C'7  h +75 .t\CTV.dial :intake C'U  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'C% h %4 .t\CSV.dial :flush C'8  +75 h  .t\CS.seek 5ml C'H'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +37 h @ C'e% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'K +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'> +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'+& h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'"'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'9% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'I'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +507 h '0 .t\CSV.dial :waste C'/ +75 h .t\CS.seek 0ml C'V'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&\ & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +7 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C'( +416 h ( M&q& h &ӷ M&%% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &{'η +91 h 'η .tHand.open M&F1 +150 h 1 M&v% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:37:25CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 16, 4 => 6, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:37:19CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'x& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(G% h %X A(|. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (`(,9 +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*j % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *'' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (_& h &,9 M&u& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b & (w +166 h (w M&2& h &w A(w& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(?& h &,9 .t\SE.seek 4.71 Pucks below Top Plate A(S' +214 h ' A(O& h &,9 M&& h &w .t\Elbow.seek 4 M&z(w +7 h (w M&y& h &w A(R& h &,9 .t\Forearm.seek 4 + 300 counts M&f'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &(/ +150 h / ."downElevator"\SE.seek :down M&l& A(0'X h &w M& % h %7 .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.seek CC M&-(ӷ +123 h 'X ."downElevator"\SE.coast A(5 +20 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&!1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)[% h % C'B& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'4 +2609 h '4 C'^% h %4 S)+% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C' @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'C9 h 9Td +100 C'T9 h 9kd +100 C'89 h 9d +100 C'9 h 9d +100 C'e9 h 9d +100 C'H9 h 9d +100 C'K9 h 9d +100 C'.9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'9 h 96d +100 C'^9 h 9Ld +100 C'+9 h 9bd +100 C' 9 h 9xd +100 C'69 h 9d +100 C'a9 h 9d +100 C'$9 h 9d +100 C'99 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'I9 h 9d +100 C'/9 h 9(d +100 C'\ 9 h 9>d +100 C'9 h 9Td +100 C'&9 h 9id +100 C'>9 h 9d +100 C'd9 h 9d +100 C'W9 h 9d +100 C'Z9 h 9d +100 C'Q9 h 9d +100 C'L9 h 9d +100 C'%9 h 9  d +100 C'<9 h 9 d +100 C'h9 h 9 *d +100 C')9 h 9 ?d +100 C'9 h 9 Td +100 C'F9 h 9 j d +100 C'q9 h 9 $d +100 C'M9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'G9 h 9 8d +100 C'|9 h 9 , +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &d1 +150 h 1 M&W% h % A(Z& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&Q'B +20 h 'B M&L& h & A(%& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &<' +22 h ' A(h% h % ."downElevator"\SE.seek :down A()'X +100 (% h %X M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +4 h 'X ."downElevator"\SE.coast A(5 +86 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'M @ +19 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'G& h & .tHand.open M&|1 +2 h 1 M&& h & M&j% h %\  .t\Elbow.seek FlushPuck::Garage M&v(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&u'η +91 h 'η .tHand.close M&x/ +150 h / M&2& h &- M&w% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'R(W +416 h (W C'& h &W .tHand.open M&(1 +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%y +151 h P%l% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%i  +38 h  .t\PS.seek 0ml P% ' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%A' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%f  C'S  h +75 .t\PSV.dial :mixloop P%z +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial :air P%!  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%[ +38 h .t\PS.seek 0ml P%B' +2609 h ' .t\PSV.dial :air P%Y  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&X& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &ӷ .t\Forearm.seek CC M&'' +90 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'n( +416 h ( M&1& h &ӷ M&*% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &g(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &s'η +91 h 'η .tHand.open M&c1 +150 h 1 M&r% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'o& h & M&p% h %\  A(% h %X A(k. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (N(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &,9 M&;& h &- M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M& & h &w A(#& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &-' +89 h ' A(:& h &,9 .t\SE.seek 3.71 Pucks below Top Plate A(7' +224 h ' A(P& h &,9 M&C& h &w .t\Elbow.seek 4 M&T(w +7 h (w M&& h &w A(e& h &,9 .t\Forearm.seek 4 + 300 counts M&m'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &./ +150 h / ."downElevator"\SE.seek :down M&& A(t'X h &w M&J% h %7 .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.seek CC M&U(ӷ +132 h 'X ."downElevator"\SE.coast A(5 +10 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'V(W +416 h (W .tHand.open M&H1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'4  +18 h  .t\CS.seek 5ml C'$'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'9' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C''4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%\ ' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%d  +151 h  .t\PS.seek :empty P%W' +2609 h ' .t\CBV.dial :waste1 C'Z @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'L  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'% @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'h  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'I ` +19 h ` .t\CSV.dial :air C'/ @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'F  +37 h  .t\CS.seek 0ml C'M'4 +2648 h '4 .t\CBV.dial :waste1 C' @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'G& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&|% h %\  A(% h %X A(j. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (&( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (Q& h & M&K& h &ӷ M&<% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &5( +143 h ( M&_& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &3' +89 h ' A(E& h & .t\SE.seek 15.71 Pucks below Top Plate A(>'L +107 h 'L M&x& h & A(2& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &w' +25 h ' .t\Elbow.hold M&q, +7 h , .t\Elbow.reconfigure TightElbow A(u- h -+L M&$&.,(\ #b .t\SE.seek 16.04 Pucks below Top Plate A(' +26 h ' M&O% h % A(v& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&?'B +21 h 'B M&a& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &(' +22 h ' .t\SE.seek 17.04 Pucks below Top Plate A({' +36 h ' M&y& h & M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&i(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&f/ +150 h / .t\CC.seek :open C'S( +416 h ( A(z& h & A( & h & M&A& h &ӷ M&!% h % .t\Forearm.seek :retract M&0'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &[( +142 h ( M&& h & A(Y& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &B' +93 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&`% h % A(X& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&b'B +21 h 'B M&@& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(R% h % ."downElevator"\SE.seek :down A(1'X +92 h 'X ."downElevator"\SE.coast A(5 +8 (*% h %X M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%,  +113 h  P%% h % .t\PS.seek 0.499ml P%c' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%r +37 h .t\PRV.dial :air P%}  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%o  +38 h .t\CBV.dial AIV C'p  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%k  +19 h  P%D% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %;' +250 h ' .t\PRV.dial :waste2 P%= @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %s' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M +2 h 1 M&:& h & M&g% h %\  .t\Elbow.seek FlushPuck::Garage M&\\(- +166 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&e/ +150 h / M&.& h &- M&% h %η .t\Forearm.seek :retract M&J'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &+' +91 h ' .t\CC.seek :closed C'm(W +415 h (W C'7& h &W .tHand.open M&T1 +150 h 1 M&t% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  C'n& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%H @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%4% h % .t\PS.seek 1.499ml P%8' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%N' +1095 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%$' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%9 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%"  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%d' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%\  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Z'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%L  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %h' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%I +76 h .t\PTV.dial :PRV,avoiding: :puck P%/ @ +75 h @ .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%F  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%G  +75 h  .t\PS.seek 2.5ml P%|' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%j  +76 h P%Q% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %K' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'< & +50 h & .t\AWV.select :waste C' +6 P%_% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'E { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %U' +1313 h ' .t\PSV.dial :air P%x  +38 h  .t\PS.seek 2.5ml P%2' +2609 h ' .t\PSV.dial :PML P%u +38 h .t\PS.seek 0ml P%O' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'v& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'a& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F* @@T .t\AIV.dial :bypass F*6  .t\PSV.dial PSR P%y +22 h  ."MFBPCR"delay 20 seconds +91 h P%l% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%3' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*5 I +129 h I F*>% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *w'6 +66 .t\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%? +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%( +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%q' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*f  +22 h ' .t\PSV.dial PSR P% +21 h  F*V% h %6 ."MFBPCR"\AS.seek 0.329ml F*z'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%A' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*! % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%Y  +38 h  .t\PS.seek 0ml P% ' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*i  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *`' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%X +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%@' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*R% h % ."MFBPCR"\AS.seek 0.469ml F*0' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%[ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%*' +2609 h ' .t\PSV.dial PSR P%' +113 h P%B% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%b' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%{  +37 h  .t\PS.seek 0ml P%,' +740 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*1 n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*&'% +336 h ' .t\PSV.dial PSR P%W +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%r' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%o' +2610 h ' .t\PSV.dial PSR P%p +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%D  +38 h  .t\PS.seek 0ml P%c' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%= +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%;' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%# +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %:'k +2678 h 'k .t\PSV.dial :waste1 P%g  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %P' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*C I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +849 h ' .t\PSV.dial :air P%e  +113 h  .t\PS.seek 2.5ml P%.' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %-' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*^  +42 h  F*\\% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *J'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*S I +43 h I F*7% h %Q ."MFBPCR"\AS.seek 0.01ml F*T' +171 h ' .t\PSV.dial :mixloop P%t +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %n' +618 h ' ."MFBPCR"\ATV.dial :waste F*H n +129 h n ."MFBPCR"\AS.seek :empty F*+'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *4' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*s  +43 h F*8% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'N  +38 h  C'9% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'"'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*\  +22 h ."MFBPCR"delay 3 seconds +300 F*d% h %_ ."MFBPCR"\AS.seek 0.434ml F*L'ݏ +109 h '4 .t\CSV.dial CSR C'%  +38 h  C'Z% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'I'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*/  +22 h F*h% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*F'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F* n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'G' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'j& h &W #tGaraging FlushPuck in Collection Clamp M&Q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&K' +91 h ' .tHand.close M&E/ +150 h / .t\CC.seek :open C')( +416 h ( M&x& h &ӷ M&U% h % .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &2(- +265 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&y1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'5& h & M&>% h %\  A( % h %X A(]. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (3(,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (?& h &,9 M&& h &- M&(% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&f& h &w A(V& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &,9 ."WCR"\SE.seek 2.71 Pucks below Top Plate A(w') +235 h ') A(q& h &,9 M&!& h &w ."WCR"\Elbow.seek 4 M&z(w +6 h (w M&Y& h &w A(i& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&'7 +23 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +19 h '&0 ."MFBPCR"delay 3 seconds +131 h / ."downElevator"\SE.seek :down M&& A(X'X h &w M&% h %7 ."WCR"\Forearm.seek :retract M&R'\ +90 h '\  ."WCR"\Elbow.seek CC M& (ӷ +79 ."MFBPCR"\ATV.dial :waste2 F*` % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +21 h (ӷ M&@& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' + h 'X ."downElevator"\SE.coast A(5 +90 h ' ."WCR"\CC.seek :closed C'*(W +416 h (W ."WCR"Hand.open M&{1 +32 h 'R ."MFBPCR"\ATV.dial :air2 F*b  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +96 h 1 M&1% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)0+ +6 h + S)W% h % C',& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'}  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +128 h n ."MFBPCR"\AS.seek :empty F* 'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*] % +43 h % F*?% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*6% h % ."MFBPCR"\AS.seek 0.579ml F*f' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*2'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*q% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*!' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Y n +85 h n ."MFBPCR"\ASV.dial :wasteR F*i  +43 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*`' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*@ n +107 h n ."MFBPCR"\ASV.dial :wasteR F*'% h %  ."MFBPCR"\AS.seek 0.09ml F*w' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*z I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*R' +625 h ' ."MFBPCR"\ATV.dial :air3 F*X % +22 h % F*)% h % ."MFBPCR"\AS.seek 0.13ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*1% h % ."MFBPCR"\AS.seek 0.23ml F*W' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +21 h % ."MFBPCR"delay 3 seconds +300 F*[% h % ."MFBPCR"\AS.seek 0.255ml F*}'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*3% h %X ."MFBPCR"\AS.seek 0.24ml F*0' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*p n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *k'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*r @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*& I +86 h I ."MFBPCR"\AS.seek 0ml F*#'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*='(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*g  +43 h  #"MFBPCR"\Swish #1 of 2 F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *C'\  +1031 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*e'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*;% h %(A ."MFBPCR"\AS.seek 0.6ml F*.'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*^% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *P' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*S  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2117h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*7  +65 h  ."MFBPCR"\ARV.dial :carrier F*t  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*T  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*s  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*4  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*"  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +107 h  ."MFBPCR"delay 7 seconds +700 F*%% h %< ."MFBPCR"\AS.seek 0.594ml F*Z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*L  +43 h  ."MFBPCR"\ASV.dial :waste1 F*F  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*I  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226195 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'<  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'j @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)_% h % ."WCR"\CBV.dial between CSV and waste2b C'm  +94 h  C'x% h % ."WCR"\CSV.dial between air and CTV C'~ ` +19 h ` ."WCR"\CS.seek 7.288ml C'K' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'v  'U  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '|'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'y  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'a' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'u  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'l  +37 h  C'>% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C']' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '?' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'5 @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'(  +94 h  C'6% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'V' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'f  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'O'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'q  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '2' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'Y  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'i  +37 h  C'!% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'A' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'@% h % ."WCR"\CSV.dial between air and CTV C'' ` +57 h ` ."WCR"\CS.seek 7.288ml C'`' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  'z  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'w  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'X' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C')  +150 h C'R& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&{% h %\  A(1% h %X A(b. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (,( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ([& h & M&W& h &ӷ M&B% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &3( +143 h ( M&}& h & A(p& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(0& h & ."WCR"\SE.seek 16.71 Pucks below Top Plate A(D'; +96 h '; M&o& h & A(k& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &&' +25 h ' ."WCR"\Elbow.hold M&c, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(#- h -+; ."WCR"\SE.seek 17.04 Pucks below Top Plate A(g' +27 h ' M&:% h % A(=& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M& 'B +21 h 'B M&C& h & A(;& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &e' +22 h ' ."WCR"\SE.seek 18.04 Pucks below Top Plate A(' +36 h ' M&.& h & M&^% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&P& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &ӷ ."WCR"\Forearm.seek CC M&7' +91 h ' ."WCR"Hand.close M&t/ +150 h / ."WCR"\CC.seek :open C'J( +416 h ( A(& h & A(\\& h & M&& h &ӷ M&H% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &T( +142 h ( M&s& h & A(8& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&4, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &91 +150 h 1 M&+% h % A("& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&$'B +21 h 'B M&N& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(M% h % ."downElevator"\SE.seek :down A(d'X +83 h 'X ."downElevator"\SE.coast A(5 +17 (%% h %X M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +89 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&Z1 +2 h 1 M&h& h & M&/% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&I% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'm& h &W .tHand.open M&x1 +150 h 1 M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'U& h &W C'v% h % .t\CSV.dial CSR C'K  +38 h  C'y% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'|'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'u% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'l'[ +463 h '[ .t\CSV.dial :waste C'> +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C']& h &W C'5% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '('p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'% h %p .t\CS.seek 7ml C'?'i +415 h 'i ."connect"\CSV.dial CTV C'f  .t\CTV.dial CSV C'  +38 h  +113  .t\CBV.dial :waste1 C'V @ +75 h @ .t\CS.seek 0ml C'q'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'O& h &W C'Y% h %4 .t\CSV.dial :flush C'i  +76 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'A% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'@'4 +766 h '4 #t\Flushing upper manifold C''& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'w% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C') 'R  +37 h +75 .t\CTV.dial :air C'{  +151 h .t\CS.seek 0ml C'1'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +507 h '0 .t\CSV.dial :waste C'[ +37 h .t\CS.seek 0ml C'W'4 +507 h '4 C'B& h &W C'}% h %4 .t\CSV.dial :flush C'p  +37 h  .t\CS.seek 3ml C'0'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'o% h %0 .t\CS.seek 5ml C'k'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'#'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C':'0 +506 h '0 .t\CSV.dial :waste C'= +76 h .t\CS.seek 0ml C'C'4 +506 h '4 #tDisinfecting external Intake with mfbkill C';& h &W C'% h %4 .t\CSV.dial CSR C'.  +151 h  C'^% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'3' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D +113 h .t\CTV.dial :intake C'&  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'P' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'S  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'g  +113 h  C'e% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'c'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 't' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '*  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'\\'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'H% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 's'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '8' +709 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'9'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'+  +38 h  C'"% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'N'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C'M .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'T  +151 h .t\CS.seek 0ml C'%'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'Z'4 +507 h '4 #t\Flushing upper manifold C'h& h &W C'/% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'$'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'L% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'd +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +507 h '0 .t\CSV.dial :waste C'4 +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'I% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Q'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'm +75 h .t\CS.seek 0ml C'x'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'~& h &W C'U% h %4 .t\CSV.dial :flush C'v  +37 h  .t\CS.seek 5ml C'K'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +37 h @ C'% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV C'u  .t\CTV.dial CSV C'|  +38 h  +113  .t\CBV.dial :waste1 C'> @ +37 h @ .t\CS.seek 0ml C'l'4 +766 h '4 #t\Flushing lower manifold's waste2b port C']& h &W C'5% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'a'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'f  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'V'4 +765 h '4 #tDisinfecting external Intake with kill C'?& h &W C'O% h %4 .t\CSV.dial CSR C'Y  +38 h  C'i% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'q'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'2' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A '!  +37 h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '''4 +606 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'@'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'`' +1372 h ' .t\CSV.dial CBV C'w +113 h .t\CS.seek 0ml C'z'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +6 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&1' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C'b( +416 h ( M&B& h &ӷ M&}% h % .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +264 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M&k1 +150 h 1 M&#% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&;1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &.(- +7 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&D/ +150 h / M&&& h &- M&3% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'c(W +416 h (W C'& h &W .tHand.open M&*1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'H% h %4 .t\CSV.dial CSR C'\\  +113 h  C's% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '8'[ +462 h '[ .t\CSV.dial :waste C'+ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '"'4 +2010 h '4 #t\Purging external Intake C'9& h &W C'N% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'T +75 h .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'%'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C' & h &W C'h% h %4 .t\CSV.dial :flush C'/  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +38 h @ C'% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'$ +112 h .t\CTV.dial :air .\CS.seek 0ml C'd'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'4'4 +506 h '4 C',& h &W C'% h %4 .t\CSV.dial :flush C'I  +38 h  .t\CS.seek 3ml C'\ '0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'j% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'Q +75 h .t\CS.seek 0ml C'm'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&J& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +6 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.seek CC M&x' +91 h ' .tHand.close M&y/ +150 h / .t\CC.seek :open C'( +416 h ( M&K& h &ӷ M&u% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +264 h (- M&X& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M& 1 +150 h 1 M&l% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST22-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:39:07CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST22-Oct-18" +263690 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2117h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*f  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*Y% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*!'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*k% h %m ."MFBPCR"\AS.seek 0.82ml F*#'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2119h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*g  +10 h  F*e% h %V ."MFBPCR"\AS.seek 0.5ml F*.'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*s  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*+' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*8  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F*N'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*"% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*%  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*L % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2119h1500ml.pcr F*d% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*F  +22 h ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *I'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*U  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*v  +64 h  F*y% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *P'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*t' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*]  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *l' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +21 h F*5% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*f'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*(% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2121h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*2  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*@'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*` n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +21 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*B n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*E% h %Fj ."MFBPCR"\AS.seek 0.524ml F*1' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.53ml F*)' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*k % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*#  +107 h  ."MFBPCR"delay 7 seconds +700 F*:% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*3  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*g'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*= % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2121h1500ml.pcr F*.% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*s'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*z% h %n ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*9  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *"'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*% % +86 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +21 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*L'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*,% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1540159219.58 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2200h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*t'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*K n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*]  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*| I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.536ml F*f'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +108 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*Y'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*? n +21 h n ."MFBPCR"delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2200h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*`  +22 h ."MFBPCR"\ATV.dial :air3 F*w % +87 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *['Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*{% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +21 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*_'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*1'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +65 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +31 h  ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *W' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*e'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*g'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*b  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *+'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2202h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*8  +11 h  F*"% h %V ."MFBPCR"\AS.seek 0.5ml F*N'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*M n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*%  +22 h ."MFBPCR"delay 2 seconds +200 F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *h'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*r % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*L' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*4'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.6ml F*\ '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*U% h % \\ ."MFBPCR"\AS.seek 0.326ml F*y'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*P % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2202h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*v @@T ."MFBPCR"\ASV.dial :SPEdelivery F*t  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*c  +150 h F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *]' +424 h ' ."MFBPCR"\ATV.dial :ASV F*~ n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*x% h % ."MFBPCR"\AS.seek 0.62ml F*|'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*5 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*l % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *(' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*f% h % ."MFBPCR"\AS.seek 0.71ml F*?' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h % H ."MFBPCR"\AS.seek 0.735ml F*i' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*q% h % Ű ."MFBPCR"\AS.seek 0.72ml F*!' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *`' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +21 h F*R% h % ."MFBPCR"\AS.seek 0.579ml F*@' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h %  ."MFBPCR"\AS.seek 0.604ml F*['\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*B  +22 h F*E% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*}  +22 h  ."MFBPCR"\AS.seek 0ml F*_'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *o'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*) % +107 h % F*1% h %V ."MFBPCR"\AS.seek 0.01ml F*#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +21 h F*p% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h % q ."MFBPCR"\AS.seek 0.605ml F*D'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +22 h F*3% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*&  +22 h  ."MFBPCR"\AS.seek 0ml F*W'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *.'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*e  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *S' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *g'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*b n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*H  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F**'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *s' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*9  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *8': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*"  +65 h  F*+% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *N'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*M n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *T' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *$': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*h  +65 h  F*r% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *d'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*F% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *,' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*L I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *I'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*4 n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*j  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*\ % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.96ml F*J'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %0 ."MFBPCR"\AS.seek 0.985ml F*y' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*u% h % ."MFBPCR"\AS.seek 0.97ml F*K'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*~ I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *x'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *5'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *X'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *G' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*(' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +128 h n ."MFBPCR"\AS.seek 0ml F*f'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*O% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Y  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +43 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*}  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*1% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +21 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +21 h F*;% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*H n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *g'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 M&6 C'5 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990369 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )  h  C' '\\ h  P% %* h  F* *7 h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ S)&\ P% 0\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *s  h  .t\SPE.configure SPEconfig F*\ 2 *z% h %V .t\AS.configure ASconfig S)9% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:40:10CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #22) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'u& h & .tHand.open M&y1 +150 h 1 M&~& h &M M&K% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &5'η +91 h 'η .tHand.close M&|/ +150 h / M&& h &- M&l% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &a' +91 h ' .t\CC.seek :closed C'O(W +416 h (W C'(& h &W .tHand.open M&f1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'A& h &W C'V% h %4 .t\CSV.dial CSR C'i  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'q'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '!% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'2& h &W C'% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'R' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'` ''  h +75 .t\CTV.dial :intake C'<  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'{'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'@  +75 h #t\Flushing upper manifold C'B& h &W C'E% h %4 .t\CSV.dial :flush C'[  +75 h  .t\CS.seek 5ml C'}'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'_% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV C') .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'1'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'k'0 +507 h '0 .t\CSV.dial :waste C': +37 h .t\CS.seek 0ml C'p'4 +507 h '4 C'o& h &W C'^% h %4 .t\CSV.dial :flush C';  +38 h  .t\CS.seek 3ml C'#'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +38 h @ C'% h %0 .t\CS.seek 5ml C'3'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'D'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'& +75 h .t\CS.seek 0ml C'0'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +7 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&S/ +150 h / .t\CC.seek :open C'H( +416 h ( M&b& h &ӷ M&% h % .t\Forearm.seek :retract M&g'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +264 h (- M&n& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 11:40:15CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 19, 4 => 3, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:40:10CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*6% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'v& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M& % ."readyMFB"\AFV.select :holdingCoil F* h %\  A(\\% h %X A(*. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (7(,9 +6 F*s% h %&K ."readyMFB"\ATV.dial :waste2 F*z % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *9' +351 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&& h &- M&"% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &+(w +166 h (w M&8& h &w A(M& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(N& h &,9 .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': A(T& h &,9 M&%& h &w .t\Elbow.seek 4 M& (w +7 h (w M&/& h &w A(h& h &,9 .t\Forearm.seek 4 + 300 counts M&r'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&Z& A(F'X h &w M&d% h %7 .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.seek CC M&4(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +11 h 'X ."downElevator"\SE.coast A(5 +80 h ' .t\CC.seek :closed C'P(W +416 h (W .tHand.open M&t1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)\ + +7 h + S)% h % C'u& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +387 h ' +50 .t\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'E9 h 9Td +100 C'`9 h 9kd +100 C'[9 h 9d +100 C'{9 h 9d +100 C'_9 h 9d +100 C'}9 h 9d +100 C')9 h 9d +100 C' 9 h 9d +100 C'19 h 9 d +100 C'9 h 9 d +100 C'B9 h 96d +100 C'k9 h 9Ld +100 C'o9 h 9bd +100 C'^9 h 9xd +100 C'9 h 9d +100 C'p9 h 9d +100 C'C9 h 9d +100 C'9 h 9d +100 C';9 h 9d +100 C'#9 h 9d +100 C'D9 h 9d +100 C'&9 h 9(d +100 C'W9 h 9>d +100 C'39 h 9Td +100 C'.9 h 9id +100 C':9 h 9d +100 C'e9 h 9d +100 C'9 h 9d +100 C'S9 h 9d +100 C'?9 h 9d +100 C'O9 h 9d +100 C'9 h 9  d +100 C'n9 h 9 d +100 C' 9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C'b9 h 9 $d +100 C'=9 h 9 (d +100 C'g9 h 9 ,d +100 C'69 h 9 0d +100 C' 9 h 9 4d +100 C'\\9 h 9 8d +100 C'*9 h 9 9 h 9\ ld +100 C'T9 h 9 pd +100 C'%9 h 9 td +100 C'h9 h 9 /xd +100 C'm9 h 9 C|d +100 C'9 h 9 Wd +100 C'9 h 9 kd +100 C'I9 h 9 d +100 C'/9 h 9 d +100 C'Z9 h 9 d +100 C'+9 h 9 d +100 C'9 h 9 d +100 C'd9 h 9 d +100 C'U9 h 9 d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'r9 h 9 1d +100 C' 9 h 9 Dd +100 C'F9 h 9 Xd +100 C',9 h 9 kd +100 C't9 h 9 ~d +100 C'H9 h 9 d +100 C'9 h 9 d +100 C'u9 h 9 d .t\CH.hold 5:00,85C C'$   P h  +30000  C'9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'\ @ +113 h @ .t\CSV.dial :air C'7 @ +38 h @ .t\CS.seek 5ml C'x'p +1372 h 'p .t\CSV.dial CTV C']  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'9  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'y @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C''4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'l +113 h .t\CBV.dial CSV C'Q  +19 h C'|% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'G  +37 h C' % h %p .t\CS.seek 7ml C'X'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'c @ +38 h @ .t\CS.seek 10ml C'5' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'Y ` +132 h ` #tLysing Complete C'A& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&V% h %\  A(i% h %X A((. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (f( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (a& h & M&L& h &ӷ M&!% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &q( +143 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &2' +89 h ' A(& h & .t\SE.seek 17.71 Pucks below Top Plate A(w'* +86 h '* M&& h & A('& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &<' +26 h ' .t\Elbow.hold M&@, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(R- h -+* .t\SE.seek 18.04 Pucks below Top Plate A(0' +27 h ' M&E% h % A(`& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&['B +20 h 'B M&{& h & A(_& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &}' +23 h ' .t\SE.seek 19.04 Pucks below Top Plate A()' +36 h ' M& & h & M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&B(ӷ +143 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'p( +416 h ( A(C& h & A(& h & M&;& h &ӷ M&#% h % .t\Forearm.seek :retract M&D'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &&( +142 h ( M&W& h & A(3& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &.' +93 h ' .t\Elbow.hold M&:, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &e1 +150 h 1 M&% h % A(S& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &?'B +20 h 'B M&O& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A( % h % ."downElevator"\SE.seek :down A('X +74 h 'X ."downElevator"\SE.coast A(5 +26 (% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'= @ +19 h @ .t\CS.seek 10ml C'g' +6 h ' .t\CSV.dial between air and CTV C'6 ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\\& h & .tHand.open M&*1 +2 h 1 M&s& h & M&z% h %\  .t\Elbow.seek FlushPuck::Garage M&-(- +166 h (- M&j& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&v/ +150 h / M&8& h &- M&M% h %η .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +264 h (ӷ M&>& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'h(W +416 h (W C'm& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%/ +151 h P%Z% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%+' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%d' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'r  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%F  h .t\PS.seek 0ml P%,' +2610 h ' .t\PSV.dial :air P%t  +37 h  .t\PS.seek 2.5ml P%H' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%u' +2609 h ' .t\PSV.dial :air P%$  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%K +37 h .t\PS.seek 0ml P%\ ' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&9' +90 h ' .tHand.close M&y/ +150 h / .t\CC.seek :open C'x( +416 h ( M&l& h &ӷ M&Q% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&c1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'Y& h & M&A% h %\  A(V% h %X A(i. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (a& h &,9 M&L& h &- M&!% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &4' +89 h ' A(& h &,9 .t\SE.seek 0.71 Pucks below Top Plate A(''K +254 h 'K A(R& h &,9 M&E& h &w .t\Elbow.seek 4 M&`(w +6 h (w M&{& h &w A(_& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down A(1'X M&2& h &w M&q% h %7 .t\Forearm.seek :retract M&w'\ +91 h '\  .t\Elbow.seek CC M&<(ӷ +143 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &[' +20 h 'X ."downElevator"\SE.coast A(5 +71 h ' .t\CC.seek :closed C'0(W +416 h (W .tHand.open M&}1 +150 h 1 M&@% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'C'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C';'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%#  +37 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :mixloop P%3 +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%^  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%B +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%e  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'S @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'O  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'D ` +19 h ` .t\CSV.dial :air C'& @ +37 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'='4 +2647 h '4 .t\CBV.dial :waste1 C'6 @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'\\& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&*% h %\  A(s% h %X A(z. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (.( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (?& h & M&)& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &n( +142 h ( M&j& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &f' +90 h ' A(5& h & .t\SE.seek 18.71 Pucks below Top Plate A(:' +77 h ' M&v& h & A(8& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &M' +25 h ' .t\Elbow.hold M&b, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+ .t\SE.seek 19.04 Pucks below Top Plate A(>' +26 h ' M&T% h % A(-& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&N'B +21 h 'B M&p& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 20.04 Pucks below Top Plate A(' +36 h ' M&/& h & M&Z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &%'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.seek CC M&d' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'r( +416 h ( A( & h & A(F& h & M&& h &ӷ M&t% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +142 h ( M&H& h & A($& h & .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 3 M&u' +93 h ' .t\Elbow.hold M&K, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&7% h % A(]& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'B +21 h 'B M&y& h & A("& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &I' +22 h ' A(h% h % ."downElevator"\SE.seek :down A(l'X +63 h 'X ."downElevator"\SE.coast A(5 +37 (Q% h %X M&9% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +89 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%% h % .t\PS.seek 0.499ml P%c' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%~ +37 h .t\PRV.dial :air P%|  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%Y  +38 h .t\CBV.dial AIV C'A  +37 h .t\PRV.dial between CBV and air P%V  +19 h  .t\PRV.dial :air P%i  +19 h  P%a% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %L' +250 h ' .t\PRV.dial :waste2 P%! @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&X(- +166 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.seek :garage M&{'η +91 h 'η .tHand.close M&_/ +150 h / M& & h &- M&2% h %η .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C''(W +415 h (W C'& h &W .tHand.open M&`1 +150 h 1 M&w% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  C'x& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%} @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%@  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%(' +1095 h ' .t\PSV.dial :air P%g  +37 h  .t\PS.seek 0ml P%C' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%#  +38 h  .tdelay 2 seconds +200 P%\ % h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %3'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%^  +75 h .tdelay 2 seconds +200 P%B% h %r .t\PS.seek 0.475ml P%e' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%W  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%S'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%O  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +76 h .t\PTV.dial :PRV,avoiding: :puck P%& @ +75 h @ .t\PS.seek 0ml P%;' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%\\  +75 h  .t\PS.seek 2.5ml P%*' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%s +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%z  +76 h P%?% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %)' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C' & +50 h & .t\AWV.select :waste C' +6 P%j% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'5 { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %<' +1313 h ' .t\PSV.dial :air P%v  +38 h  .t\PS.seek 2.5ml P%8' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%T' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '-& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'p& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*m @@T .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%/ +22 h  ."MFBPCR"delay 20 seconds +91 h P%Z% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%f' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*n I +129 h I F*:% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *M'6 +66 .t\PSV.dial :waste1 P%+  +37 h  .t\PS.seek 0ml P%U' +1597 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%b' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%% +21 h  F*0% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%F% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*t % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%$  +38 h  .t\PS.seek 0ml P%d' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%] +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +217 ."MFBPCR"\ATV.dial :AIV F*" I +43 h I F*h% h % ."MFBPCR"\AS.seek 0.469ml F*,' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Q' +2609 h ' .t\PSV.dial PSR P%9 +113 h P%u% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P% ' +740 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*l n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*I% h % ."MFBPCR"\AS.seek 0.041ml F*.'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%~' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%| +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%Y' +2610 h ' .t\PSV.dial PSR P%A +113 h P%V% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%a  +38 h  .t\PS.seek 0ml P%c' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%! +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%L' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %R' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*E I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *K'V +849 h ' .t\PSV.dial :air P%_  +113 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%2 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %G' +5107 h ' .t\PSV.dial :air P%{  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %4' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*k  +42 h  F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *q'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*r I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*`' +171 h ' .t\PSV.dial :mixloop P%w +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %x' +618 h ' ."MFBPCR"\ATV.dial :waste F*} n +129 h n ."MFBPCR"\AS.seek :empty F*o'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*@  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*[% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *g'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'(  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'#'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'\ ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  '^  +38 h  +113  .t\CBV.dial :waste1 C'B @ +37 h @ .t\CS.seek 0ml C'3'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*W  +22 h ."MFBPCR"delay 3 seconds +300 F*e% h %_ ."MFBPCR"\AS.seek 0.434ml F*O'ݏ +109 h '4 .t\CSV.dial CSR C'  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'D'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*&  +22 h F* % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*; n +108 h n F*6% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'\\' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C's'4 +2647 h '4 C'z& h &W #tGaraging FlushPuck in Collection Clamp M&?& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&)' +91 h ' .tHand.close M&5/ +150 h / .t\CC.seek :open C'( +416 h ( M&v& h &ӷ M&<% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &8(- +265 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&/1 +150 h 1 M&Z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +90 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C'n& h & M&:% h %\  A(+% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (f(,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (N& h &,9 M&U& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &>(w +166 h (w M&& h &w A(0& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(F& h &,9 ."WCR"\SE.seek -0.29 Pucks below Top Plate A(M'\\ +264 h '\\ A(b& h &,9 M&t& h &w ."WCR"\Elbow.seek 4 M& (w +7 h (w M&$& h &w A(& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&'7 +12 h '&0 ."MFBPCR"delay 3 seconds +10 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(H'X M&]& h &w M&"% h %7 ."WCR"\Forearm.seek :retract M&h'\ +91 h '\  ."WCR"\Elbow.seek CC M&d(ӷ +49 ."MFBPCR"\ATV.dial :waste2 F*7 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +51 h (ӷ M&y& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &u' +31 h 'X ."downElevator"\SE.coast A(5 +60 h ' ."WCR"\CC.seek :closed C'Q(W +416 h (W ."WCR"Hand.open M&1 +2 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +126 h 1 M&l% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S),+ +7 h + S)% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'|  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +108 h % F*%% h %V ."MFBPCR"\AS.seek 0.59ml F*7' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*y n +107 h n ."MFBPCR"\ASV.dial :wasteR F*9% h %  ."MFBPCR"\AS.seek 0.09ml F*M' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*h' +625 h ' ."MFBPCR"\ATV.dial :air3 F*H % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*l% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*|'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*f% h %X ."MFBPCR"\AS.seek 0.24ml F*,' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*A n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*V  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *i'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*~ @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*. I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*!'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *E'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*_'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*L% h %(A ."MFBPCR"\AS.seek 0.6ml F* '\  +1032 h '\  F*{% h %\  ."MFBPCR"\AS.seek 0.5ml F*G'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*k% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *R' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*2 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *K'V +3472 h 'V ."MFBPCR"\SPE.coast F*r  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2217h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +65 h  ."MFBPCR"\ARV.dial :carrier F*w  +10 h  F*q% h %V ."MFBPCR"\AS.seek 0.5ml F*X'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*P  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*(' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*#  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*=' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*B % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*C'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*e  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*3% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*6  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*D  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226225 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'z @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)j% h % ."WCR"\CBV.dial between CSV and waste2b C''  +94 h  C'v% h % ."WCR"\CSV.dial between air and CTV C'1 ` +19 h ` ."WCR"\CS.seek 7.288ml C')' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'-  '<  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '*'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'/  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'p' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Z  +38 h  C':% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'n @ +75 h @ +50 S)+% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'U ` +57 h ` ."WCR"\CS.seek 7.288ml C'0' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'F  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'T'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'b  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '8' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'$  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C't% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ']' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'> @ +76 h @ +50 S)%% h % ."WCR"\CBV.dial between CSV and waste2b C'"  +94 h  C'y% h % ."WCR"\CSV.dial between air and CTV C'9 ` +56 h ` ."WCR"\CS.seek 7.288ml C'7' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'M  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'H' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'h& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(l% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ( ( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&u% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &f( +143 h ( M&|& h & A(A& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &V' +89 h ' A(,& h & ."WCR"\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' M&Y& h & A(i& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &.' +25 h ' ."WCR"\Elbow.hold M&c, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 20.04 Pucks below Top Plate A(' +27 h ' M&% h % A(!& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&E& h & A(L& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &_' +22 h ' ."WCR"\SE.seek 21.04 Pucks below Top Plate A({' +36 h ' M& & h & M&k% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +90 h '\  ."WCR"\Elbow.seek CC M&2(ӷ +142 h (ӷ M&R& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&w/ +150 h / ."WCR"\CC.seek :open C'q( +416 h ( A(K& h & A(X& h & M&& h &ӷ M&}% h % ."WCR"\Forearm.seek :retract M&@'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &`( +142 h ( M&& h & A([& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&P, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&o% h % A(#& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&^'B +21 h 'B M&(& h & A(B& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' A(=% h % ."downElevator"\SE.seek :down A(e'X +53 h 'X ."downElevator"\SE.coast A(5 +47 (% h %X M&C% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'3& h & .tHand.open M&S1 +2 h 1 M& & h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&g(- +166 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&6'η +91 h 'η .tHand.close M& / +150 h / M&;& h &- M&D% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'j(W +416 h (W C''& h &W .tHand.open M&v1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'<& h &W C'-% h % .t\CSV.dial CSR C')  +38 h  C'/% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'*'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Z'[ +463 h '[ .t\CSV.dial :waste C': +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'n% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'U% h %p .t\CS.seek 7ml C'N'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'F  +38 h  +113  .t\CBV.dial :waste1 C'0 @ +75 h @ .t\CS.seek 0ml C'b'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'T& h &W C'$% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C't'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'% h %p .t\CS.seek 7ml C'>'i +415 h 'i ."connect"\CSV.dial CTV C'%  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'"  +75 h  .t\CS.seek 0ml C'y'4 +766 h '4 #t\Flushing upper manifold C'9& h &W C']% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'M% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'h  +37 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'l'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'u& h &W C'|% h %4 .t\CSV.dial :flush C'A  +37 h  .t\CS.seek 3ml C','0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'Y @ +38 h @ C'i% h %0 .t\CS.seek 5ml C'd'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'! +76 h .t\CS.seek 0ml C'E'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'L& h &W C' % h %4 .t\CSV.dial CSR C'k  +151 h  C'I% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'V'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'f' +415 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a +113 h .t\CTV.dial :intake C'.  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'R' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'r  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'_% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'c'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'w' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  'Q  +38 h  +113  .t\CBV.dial :waste1 C'K @ +38 h @ .t\CS.seek 0ml C'X'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'}% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'2  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'o  +38 h  C'#% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '('P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'B' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'= '@  +38 h +75 .t\CTV.dial :air C'`  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +507 h '0 .t\CSV.dial :waste C'3 +37 h .t\CS.seek 0ml C'S'4 +507 h '4 #t\Flushing upper manifold C' & h &W C'&% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'^'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'e% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'P +38 h .t\CS.seek 0ml C' '4 +506 h '4 C';& h &W C'D% h %4 .t\CSV.dial :flush C'W  +38 h  .t\CS.seek 3ml C'6'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'z @ +38 h @ C's% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'?'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'' +75 h .t\CS.seek 0ml C'v'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'1& h &W C'<% h %4 .t\CSV.dial :flush C'-  +37 h  .t\CS.seek 5ml C')'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'% h %p .t\CS.seek 7ml C'H'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'*  +38 h  +113  .t\CBV.dial :waste1 C': @ +37 h @ .t\CS.seek 0ml C'Z'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'n% h %4 .t\CSV.dial :flush C'+  +76 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'U% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'F  +76 h  .t\CS.seek 0ml C'0'4 +765 h '4 #tDisinfecting external Intake with kill C'N& h &W C'T% h %4 .t\CSV.dial CSR C'$  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'b'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'8' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 't  +37 h +76 .t\CTV.dial :intake C'%  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'"' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'>  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '9'4 +606 h '4 .t\CSV.dial CSR C']  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'y'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'7' +1372 h ' .t\CSV.dial CBV C'M +113 h .t\CS.seek 0ml C''4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&h& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&u& h &ӷ M&|% h % .t\Forearm.seek :retract M&A'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &,'η +91 h 'η .tHand.open M&d1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&L1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &k(- +7 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&a/ +150 h / M&.& h &- M&f% h %η .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'c(W +416 h (W C'G& h &W .tHand.open M&Q1 +150 h 1 M&K% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'}% h %4 .t\CSV.dial CSR C'X  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '['[ +462 h '[ .t\CSV.dial :waste C'o +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +2010 h '4 #t\Purging external Intake C'& h &W C'(% h %4 .t\CSV.dial :flush C'@  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'` +75 h .t\CTV.dial :intake C'B  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'3  +75 h #t\Flushing upper manifold C'C& h &W C' % h %4 .t\CSV.dial :flush C'&  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +38 h @ C'{% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CBV C'^ .t\CBV.dial CSV +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'\ +38 h .t\CS.seek 0ml C'P'4 +506 h '4 C' & h &W C';% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 3ml C'W'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'z% h %0 .t\CS.seek 5ml C's'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'? +75 h .t\CS.seek 0ml C'''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'( +416 h ( M&)& h &ӷ M&% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &n'η +91 h 'η .tHand.open M&+1 +150 h 1 M&Z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST23-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:41:58CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST23-Oct-18" +263690 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2217h1500ml.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *N'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*$% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*t'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*8'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*>  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +65 h  F*]% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *5' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*u% h % ."MFBPCR"\AS.seek 0.81ml F*|'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2219h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*_% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*K  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*2  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*o' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*[  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +107 h  ."MFBPCR"delay 7 seconds +700 F*#% h %< ."MFBPCR"\AS.seek 0.594ml F*`'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*B n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*C  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*e % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2219h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*O  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *D'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*6'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*g% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*<  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +64 h  F*/% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +86 h % F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +21 h F*n% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*T  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2221h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*9% h %V ."MFBPCR"\AS.seek 0.5ml F*y'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *h'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*u n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*Y I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*d % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*L'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*,  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*f  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*_  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2221h1500ml.pcr F*k% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*r  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*Q% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*['q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +64 h  F*@% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*3  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *{' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F*e'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V @CEST1540245619.58 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *-'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2300h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*R% h %V ."MFBPCR"\AS.seek 0.5ml F*w'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*) n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +21 h ."MFBPCR"delay 2 seconds +200 F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *H'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F** I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*U n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*m' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*$'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*N n +21 h n ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*%' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*>  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*8'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2300h1500ml.pcr F*]% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*7  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +87 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +21 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*j'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*|% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*d  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*i  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*a  +31 h  ."MFBPCR"\ATV.dial :air3 F*E % +87 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Q' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2302h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*@  +42 h  ."MFBPCR"\ARV.dial :carrier F*[  +11 h  F*#% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*= n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *`'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*3  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*& I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*~ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*e' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*O  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +22 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*P'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*?' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*g  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*6  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*/'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*R % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2302h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*- @@T *w  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*c  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F*1 n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*+  +43 h  F*v% h % ."MFBPCR"\AS.seek 0.62ml F**'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*n n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *U'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*\\'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*Z % +43 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*m% h % ."MFBPCR"\AS.seek 0.53ml F*T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*N' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*$% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*b% h % Ű ."MFBPCR"\AS.seek 0.72ml F*t' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*> n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*"% h % zG ."MFBPCR"\AS.seek 0.365ml F*%' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*] % +43 h % F*8% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *7' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +21 h F*h% h % ."MFBPCR"\AS.seek 0.579ml F*y' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*5% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*Y n +85 h n ."MFBPCR"\ASV.dial :wasteR F*|  +22 h  ."MFBPCR"\AS.seek 0ml F*j'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *i'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*l% h %V ."MFBPCR"\AS.seek 0.01ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*A% h % ."MFBPCR"\AS.seek 0.58ml F* ' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h % q ."MFBPCR"\AS.seek 0.605ml F*a'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*f% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*,' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*V n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*.  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *k'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*_  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *r' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*G n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *K'R +202 h 'R ."MFBPCR"\SPE.coast F*}  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Q'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *[': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*#  +65 h  F*o% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *('Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*= n +64 h n F*B% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *`' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*3 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*C  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *^': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*~% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*{ n +65 h n F*O% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*e I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*;  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *D'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*P n +151 h n ."MFBPCR"\AS.seek 0ml F*s'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*z  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*W % +43 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*?% h % ."MFBPCR"\AS.seek 0.96ml F*q'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h %0 ."MFBPCR"\AS.seek 0.985ml F*/' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*)'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*1 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *v'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *n'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*Z% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *\\' +9988 h ' ."MFBPCR"\ATV.dial AIV F*m I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*p n +128 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*T% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*$  +22 h ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *b'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*>% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*%'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*l% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*A% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +21 h F*L% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*a' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*} n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*K % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast .\CS.coast S)5 C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990369 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' 'X h  P% %Q h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ F*\ 9\ C' 2\ P% 0\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'@& h & S)#.0X 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:57CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #23) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&/1 +150 h 1 M&1& h &M M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &+(- +166 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &n'η +91 h 'η .tHand.close M&*/ +150 h / M&U& h &- M&Z% h %η .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +264 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'0% h %4 .t\CSV.dial CSR C'  +150 h  C'>% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'b'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 't% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'" +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ']'4 +2009 h '4 #t\Purging external Intake C'8& h &W C'%% h %4 .t\CSV.dial :flush C'M  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'h' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7  '9 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'y  +75 h #t\Flushing upper manifold C'u& h &W C'5% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'|'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'j% h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'l'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'd'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'A'4 +507 h '4 C'i& h &W C'I% h %4 .t\CSV.dial :flush C'L  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C' % h %0 .t\CS.seek 5ml C'f'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'a'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C','4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&G' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C'}( +416 h ( M&& h &ӷ M&K% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &J(- +264 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &4'η +91 h 'η .tHand.open M&'1 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 11:43:03CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 0, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:57CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 5 into Collection Clamp h %&K C'-& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(X% h %X A(Q. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (@& h &; M&2& h &- M&#% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &o(M +166 h (M M&[& h &M A(=& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &B'x +90 h 'x A((& h &; .t\SE.seek 20.71 Pucks below Top Plate A(3' +56 h ' A(`& h &; M&& h &M .t\Elbow.seek 5 M&C(M +7 h (M M&&& h &M A(S& h &; .t\Forearm.seek 5 + 300 counts M&~' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &{/ +150 h / ."downElevator"\SE.seek :down A(O'X M& & h &M M&% h % .t\Forearm.seek :retract M& '\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&P(ӷ +143 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +90 h ' .t\CC.seek :closed C'R(W +416 h (W .tHand.open M&w1 +150 h 1 M&^% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)W+ +7 h + S)s% h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +1306 h ' ."readyMFB"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."readyMFB"delay 1 second +100 .\ATV.dial :waste F*p n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*t  h  ."readyMFB"\ASV.dial :waste1 F*b  h +1565 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'"% h %4 S) % h % S)8% h % .tIntake.select :close S)0 +7 )%% h % .t\SSV.dial :exhaust S)M  +75 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9xd +100 C'79 h 9|d +100 C'99 h 9d +100 C'y9 h 9d +100 C'h9 h 9'd +100 C',9 h 9>d +100 C'59 h 9Td +100 C'9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'j9 h 9d +100 C'|9 h 9d +100 C'9 h 9d +100 C'Y9 h 9d +100 C'l9 h 9 d +100 C']9 h 9 d +100 C'u9 h 96d +100 C'd9 h 9Ld +100 C'i9 h 9bd +100 C'I9 h 9xd +100 C'9 h 9d +100 C'A9 h 9d +100 C'E9 h 9d +100 C' 9 h 9d +100 C'L9 h 9d +100 C'9 h 9d +100 C'a9 h 9d +100 C'.9 h 9(d +100 C'9 h 9>d +100 C'f9 h 9Td +100 C'k9 h 9id +100 C'9 h 9d +100 C'_9 h 9d +100 C'V9 h 9d +100 C'r9 h 9d +100 C'N9 h 9d +100 C'T9 h 9d +100 C'K9 h 9  d +100 C'G9 h 9 d +100 C'F9 h 9 *d +100 C':9 h 9 ?d +100 C'9 h 9 Td +100 C''9 h 9 j d +100 C'9 h 9 $d +100 C'!9 h 9 (d +100 C'9 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'X9 h 9 8d +100 C'Q9 h 9 & h &,9 A(7& h &,9 M&9& h &ӷ M&y% h % .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &,(w +143 h (w M&5& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &' +92 h '  .t\Elbow.hold M&, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &j1 +150 h 1 M&|% h %  A(& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&Y'7 +21 h '7 M&l& h &w A(]& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &u' +22 h ' A(d% h %Z ."downElevator"\SE.seek :down A(i'X +100 (I% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'E @ + A(6 +17 h @ .t\CS.seek 10ml C' ' +7 h ' .t\CSV.dial between air and CTV C'L ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'a& h & .tHand.open M&.1 +2 h 1 M&& h &w M&f% h %\  .t\Elbow.seek FlushPuck::Garage M&k(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&r/ +150 h / M&N& h &- M&T% h %η .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C''(W +416 h (W C'& h &W .tHand.open M&!1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%X% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%Q' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%x +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%z' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%@  C'2  h +76 .t\PSV.dial :mixloop P%- +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%[  h .t\PS.seek 0ml P%=' +2609 h ' .t\PSV.dial :air P%(  +38 h  .t\PS.seek 2.5ml P%#' +2610 h ' .t\PSV.dial :mixloop P%J +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%&  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%` +38 h .t\PS.seek 0ml P% ' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&4& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +6 h (ӷ M&S& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&o' +91 h ' .tHand.close M&B/ +150 h / .t\CC.seek :open C'D( +416 h ( M&<& h &ӷ M&;% h % .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &3(- +264 h (- M&O& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&w1 +150 h 1 M&}% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'& h & M&^% h %\  A(% h %X A(). h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (W(; +201 h (; .t\SC.reconfigure SCloose A($.(\ 1~b ( & h &; M&6& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &g(M +166 h (M M&& h &M A(/& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &v'x +89 h 'x A(Z& h &; .t\SE.seek 19.71 Pucks below Top Plate A(?' +66 h ' A(*& h &; M&U& h &M .t\Elbow.seek 5 M&\\(M +6 h (M M&+& h &M A(m& h &; .t\Forearm.seek 5 + 300 counts M&c' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &1/ +150 h / ."downElevator"\SE.seek :down A(H'X M&$& h &M M&% h % .t\Forearm.seek :retract M&0'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &p' +91 h ' .t\CC.seek :closed C'"(W +416 h (W .tHand.open M& 1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +150 h  .t\CSV.dial CTV C'R  +19 h  .t\CS.seek 5ml C'>'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'7' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%y  +38 h  .t\PS.seek 2.5ml P%5' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%8  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%b +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%j  +151 h  .t\PS.seek :empty P%|' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'l  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'] @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'd  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'h ` +19 h ` .t\CSV.dial :air C', @ +37 h @ .t\CS.seek 10ml C'I' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'Y @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'E  +151 h C't& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&L% h %\  A(% h %X A(a. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (.(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&f& h &ӷ M&u% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +143 h (w M&_& h &w A(i& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(n& h &,9 .t\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ M&r& h &w A(N& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &T' +26 h '  .t\Elbow.hold M&A, +6 h ,w .t\Elbow.reconfigure TightElbow A(V- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(F' +27 h ' M&:% h %  A(k& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&K'7 +20 h '7 M&M& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &!' +23 h ' .t\SE.seek 1.04 Pucks below Top Plate A(' +36 h ' M&& h &w M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'2( +416 h ( A(-& h &,9 A([& h &,9 M&z& h &ӷ M&(% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &J(w +142 h (w M&#& h &w A(&& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &' +93 h '  .t\Elbow.hold M&`, +6 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&4% h %  A(S& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&'7 +20 h '7 M&B& h &w A(G& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +22 h ' A('% h % ."downElevator"\SE.seek :down A(<'X +100 (;% h %S M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  + A(6 +112 h  P%{% h % .t\PS.seek 0.499ml P%w' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%} +38 h .t\PRV.dial :air P%~  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +37 h .t\CBV.dial AIV C'^  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%)  +19 h  P% % h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %6' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&/1 +2 h 1 M&Z& h &w M&3% h %\  .t\Elbow.seek FlushPuck::Garage M&*(- +166 h (- M&U& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.seek :garage M&m'η +91 h 'η .tHand.close M&s/ +150 h / M&1& h &- M&$% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &g(ӷ +264 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &e' +91 h ' .t\CC.seek :closed C'c(W +416 h (W C'?& h &W .tHand.open M&H1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  C'D& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%R% h % .t\PS.seek 1.499ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%W' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%>' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%7 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%y  +38 h  .tdelay 2 seconds +200 P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%8  +75 h .tdelay 2 seconds +200 P%b% h %r .t\PS.seek 0.475ml P%j' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%5  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%l  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%]  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %d' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%h +75 h .t\PTV.dial :PRV,avoiding: :puck P%, @ +76 h @ .t\PS.seek 0ml P%9' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%I' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Y +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%E' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%t  +76 h  .t\PS.seek 2.5ml P%L' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%a  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %f' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'u & +50 h & .t\AWV.select :waste C' +7 P%_% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %i'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'n { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%P  +37 h  .t\PS.seek 2.5ml P%r' +2610 h ' .t\PSV.dial :PML P%N +38 h .t\PS.seek 0ml P%V' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 ':& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'k& F*M @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +22 h  ."MFBPCR"delay 20 seconds +91 h P%X% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +128 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *T'6 +66 .t\PSV.dial :waste1 P%Q  +38 h  .t\PS.seek 0ml P%x' +1597 h ' .t\PSV.dial PSR P%K +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%F' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%! +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%A' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +22 h ' .t\PSV.dial PSR P% +21 h  F*"% h %6 ."MFBPCR"\AS.seek 0.329ml F*-'ͯ +92 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%z' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*( % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *#'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%&  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *4' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%S +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%B' +217 ."MFBPCR"\ATV.dial :AIV F*G I +43 h I F*'% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%;' +2610 h ' .t\PSV.dial PSR P%o +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P% ' +740 h ' ."MFBPCR"delay 5 seconds +500 F*{9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*<% h % ."MFBPCR"\AS.seek 0.041ml F*.'% +336 h ' .t\PSV.dial PSR P%| +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%}' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%~ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%^ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%)' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%w' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%6' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %Z'k +2678 h 'k .t\PSV.dial :waste1 P%3  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %U' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*+ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *`'V +850 h ' .t\PSV.dial :air P%s  +113 h  .t\PS.seek 2.5ml P%1' +2609 h ' .t\PSV.dial :mixloop P%$ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %O' +5107 h ' .t\PSV.dial :air P%m  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %v' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*2 I +43 h I F*?% h %Q ."MFBPCR"\AS.seek 0.01ml F*H' +171 h ' .t\PSV.dial :mixloop P%\\ +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %D' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*e'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*%  +85 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *R' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*p% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b * '_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'W  +38 h  C'7% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'y'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1626 h ' ."connect"\CSV.dial CTV C'>  .t\CTV.dial CSV C'8  +37 h  +113  .t\CBV.dial :waste1 C'b @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*5  +22 h ."MFBPCR"delay 3 seconds +300 F*j% h %_ ."MFBPCR"\AS.seek 0.434ml F*l'ݏ +110 h '4 .t\CSV.dial CSR C']  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'h'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*,  +21 h F*d% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*Y% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C't' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2648 h '4 C'a& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &L(ӷ +6 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'i( +416 h ( M&P& h &ӷ M&% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&X% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'& h & M&% h %\  A(Q% h %X A(\ . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b ((; +200 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (K& h &; M&x& h &- M&!% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &F(M +166 h (M M&@& h &M A("& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &'x +90 h 'x A([& h &; ."WCR"\SE.seek 18.71 Pucks below Top Plate A(T' +77 h ' A(A& h &; M&(& h &M ."WCR"\Elbow.seek 5 M&-(M +6 h (M M&&& h &M A(& h &; ."WCR"\Forearm.seek 5 + 300 counts M&' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &z/ +150 h / ."downElevator"\SE.seek :down A(#'X M&S& h &M M&G% h % ."WCR"\Forearm.seek :retract M&''\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&4& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' +91 h ' ."WCR"\CC.seek :closed C'o(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +370 h (W ."WCR"Hand.open M&1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &{'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *<' +30 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +6 h + S)|% h % C' & h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'~  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *8'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*b I +43 h I F* % h %N ."MFBPCR"\AS.seek 0.109ml F*5'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*j n +173 h n ."MFBPCR"\ASV.dial :SPEelution F*]  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b * ' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *,' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*d% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *l' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*9% h % ."MFBPCR"\AS.seek 0.279ml F*Y'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*E % +42 h % F*t% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *a'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F* I +21 h I F*u% h %v ."MFBPCR"\AS.seek 0.059ml F*_'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*n % +21 h % F*g% h %H ."MFBPCR"\AS.seek 0.069ml F*0' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*P% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*:% h % q ."MFBPCR"\AS.seek 0.794ml F*k' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*X'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*N I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*Q'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*\ % +43 h % F*K% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *x' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*@' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +21 h % ."MFBPCR"delay 3 seconds +300 F*[% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +21 h F*(% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*&' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +43 h  ."MFBPCR"\AS.seek 0ml F*z'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *G'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*F % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*4' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*J n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*T' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*# I +86 h I F*-% h % ."MFBPCR"\AS.seek 0.12ml F*'' +625 h ' ."MFBPCR"\ATV.dial :air3 F*i % +21 h % F*I% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*|' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h % ."MFBPCR"\AS.seek 0.255ml F*B'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*=' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*^  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 * % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *)'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*} @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*. I +86 h I ."MFBPCR"\AS.seek 0ml F*/'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  #"MFBPCR"\Swish #1 of 2 F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *+'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*s'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*6% h %(A ."MFBPCR"\AS.seek 0.6ml F*1'\  +1031 h '\  F*m% h %\  ."MFBPCR"\AS.seek 0.5ml F*O'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *U' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*$ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3472 h 'V ."MFBPCR"\SPE.coast F*2  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2317h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*?  +64 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F**'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *%'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*H  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *e'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*R  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*W' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*y  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*b % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*8'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +107 h  ."MFBPCR"delay 7 seconds +700 F*]% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*5 n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.65ml F*9' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +42 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*E% h % \\ ."MFBPCR"\AS.seek 0.326ml F*t'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226038 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'u  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'a @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'n' +910 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'g @ +75 h @ +50 S)_% h % ."WCR"\CBV.dial between CSV and waste2b C'c  +94 h  C'P% h % ."WCR"\CSV.dial between air and CTV C'0 ` +19 h ` ."WCR"\CS.seek 7.288ml C'f' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C':  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'L'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'N  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'X  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'\ ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)Q% h % ."WCR"\CBV.dial between CSV and waste2b C'!  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'x ` +56 h ` ."WCR"\CS.seek 7.288ml C'"' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'[  '@  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'A'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'(  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'r' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'V  +37 h  C'&% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'z' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'F @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'G  +94 h  C'J% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'4' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'#  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '-'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'T  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'i' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +151 h C''& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ( (,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (~& h &,9 M&|& h &ӷ M&;% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +143 h (w M&B& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &^' +89 h ' A(=& h &,9 ."WCR"\SE.seek 0.71 Pucks below Top Plate A( 'K +254 h 'K M&& h &w A()& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &.' +25 h '  ."WCR"\Elbow.hold M&w, +6 h ,w ."WCR"\Elbow.reconfigure TightElbow A(/- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(3' +27 h ' M&Z% h %  A(& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&'7 +21 h '7 M&+& h &w A(6& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &s' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(m' +36 h ' M&1& h &w M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +89 h '\  ."WCR"\Elbow.seek CC M&$(ӷ +143 h (ӷ M&U& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ ."WCR"\Forearm.seek CC M&?' +91 h ' ."WCR"Hand.close M&\\/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(`& h &,9 A(*& h &,9 M&<& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&%'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &H(w +142 h (w M&C& h &w A(p& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &' +93 h '  ."WCR"\Elbow.hold M&R, +6 h ,w ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &71 +150 h 1 M&e% h %  A(y& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&>'7 +20 h '7 M&W& h &w A(b& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b & ' +23 h ' A(% h % ."downElevator"\SE.seek :down A(j'X +100 (]% h %B M&8% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 + A(6 + h 1 M&d& h &w M&,% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +165 h (- M&l& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&Y'η +91 h 'η .tHand.close M&E/ +150 h / M&9& h &- M&h% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +265 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'c& h &W .tHand.open M&P1 +150 h 1 M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'% h % .t\CSV.dial CSR C'f  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'L'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'N% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'X'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'Q  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'x% h %p .t\CS.seek 7ml C'K'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  '[  +37 h  +113  .t\CBV.dial :waste1 C'" @ +76 h @ .t\CS.seek 0ml C'('4 +766 h '4 #t\Flushing lower manifold's waste2b port C'A& h &W C'% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +38 h @ C'z% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'G  +76 h  .t\CS.seek 0ml C'J'4 +766 h '4 #t\Flushing upper manifold C'& h &W C'S% h %4 .t\CSV.dial :flush C'#  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'T% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I ''  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'~ +37 h .t\CS.seek 0ml C'|'4 +507 h '4 C';& h &W C'B% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'='0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'{% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C')'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C'Z +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'+& h &W C'6% h %4 .t\CSV.dial CSR C'1  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'^'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'.  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'U' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'2  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'3  +113 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\\' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'O  'o  +37 h  +113  .t\CBV.dial :waste1 C'` @ +38 h @ .t\CS.seek 0ml C'*'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'<  +37 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'C'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'p' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  +37 h  .t\CBV.dial :waste2b C'$  +76 h  .t\CS.seek 0ml C'7'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'e  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'W'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'b' +709 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'%  +38 h +75 .t\CTV.dial :air C'H  +151 h .t\CS.seek 0ml C']'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'd& h &W C',% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'm @ +38 h @ C' % h %p .t\CS.seek 7ml C'l'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'j'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'R +38 h .t\CS.seek 0ml C'E'4 +506 h '4 C'9& h &W C'h% h %4 .t\CSV.dial :flush C'5  +38 h  .t\CS.seek 3ml C'Y'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +37 h @ C'% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'c +75 h .t\CS.seek 0ml C'P'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'0& h &W C':% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'g% h %p .t\CS.seek 7ml C'i'i +415 h 'i ."connect"\CSV.dial CTV C'N  .t\CTV.dial CSV C'L  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'X'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'Q  +75 h  .t\CS.seek 5ml C'k'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'x% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +37 h  .t\CBV.dial :waste2b C'[  +76 h  .t\CS.seek 0ml C'"'4 +766 h '4 #tDisinfecting external Intake with kill C'K& h &W C'A% h %4 .t\CSV.dial CSR C'  +37 h  C'V% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'('L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'r' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z '&  +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'G' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'F  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 .t\CSV.dial CSR C'S  +113 h  C'#% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'J'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'4' +1372 h ' .t\CSV.dial CBV C'T +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&'& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'( +416 h ( M&;& h &ӷ M&B% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &|(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&1 +150 h 1 M&)% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +90 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&+1 +2 h 1 M&6& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &1(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M& / +150 h / M&.& h &- M&% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +264 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'w(W +416 h (W C'O& h &W .tHand.open M&o1 +150 h 1 M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'<& h &W C' % h %4 .t\CSV.dial CSR C'*  +113 h  C'C% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'?'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '$% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'p'[ +463 h '[ .t\CSV.dial :waste C'e +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'y'4 +2010 h '4 #t\Purging external Intake C'7& h &W C'W% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'H .t\CBV.dial CSV +76 h .t\CTV.dial :intake C'b  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C']'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'8& h &W C'd% h %4 .t\CSV.dial :flush C',  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'm% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'> +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +506 h '0 .t\CSV.dial :waste C'j +38 h .t\CS.seek 0ml C'R'4 +506 h '4 C' & h &W C'9% h %4 .t\CSV.dial :flush C'h  +38 h  .t\CS.seek 3ml C'5'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +37 h @ C'a% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'Y'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'c'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +7 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&P' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'g( +416 h ( M&f& h &ӷ M&N% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&X% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST24-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:44:44CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST24-Oct-18" +263637 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2317h1500ml.pcr F*x% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F*[ % +87 h % F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *K'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*V'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +65 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +31 h  ."MFBPCR"\ATV.dial :air3 F*~ % +87 h % F*u% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*;% h % ."MFBPCR"\AS.seek 0.81ml F*B'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*{'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Z'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2319h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ARV.dial :carrier F*3  +11 h  F*s% h %V ."MFBPCR"\AS.seek 0.5ml F*1'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*<  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*C  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*$  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*e' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*p  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.536ml F*W'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +108 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*b n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*8  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*m% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2319h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*l  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*9% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *h'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*E% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*:  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*Q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *X' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*M'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*!% h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*A  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2321h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*J'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*~  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*u  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*; n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.53ml F*I' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +107 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*6 n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*=  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2321h1500ml.pcr F*1% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*2  +21 h ."MFBPCR"\ATV.dial :air3 F*O % +87 h % F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*o% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*7  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*e  +65 h  F*%% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *y'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*] % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*>% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *R'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V @CEST1540332013.88 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2400h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*f n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*\  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*0  +22 h ."MFBPCR"delay 2 seconds +200 F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*L I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*X' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*x n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*[  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*K n +21 h n ."MFBPCR"delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.6ml F*('\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*&  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*F  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2400h1500ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *J'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*4  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*-'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*_'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*B% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*I% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *|' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +21 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*s'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *e'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2402h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +11 h  F*y% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*]  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *d'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*, I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*>% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*} % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*l  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +22 h  ."MFBPCR"delay 7 seconds +700 F*9% h %< ."MFBPCR"\AS.seek 0.594ml F*R'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +21 h n ."MFBPCR"delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.6ml F*5'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2402h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*\\  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*w  +151 h F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\ ' +424 h ' ."MFBPCR"\ATV.dial :ASV F*0 n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*Q  +43 h  F*P% h % ."MFBPCR"\AS.seek 0.62ml F*L'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *x'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *!' +424 h ' ."MFBPCR"\ATV.dial :air3 F*M% h % ."MFBPCR"\AS.seek 0.53ml F*A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*@% h % ."MFBPCR"\AS.seek 0.71ml F*K' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*V' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +21 h F*(% h % Ű ."MFBPCR"\AS.seek 0.72ml F*&' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*F n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*G% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*# n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *4' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*'% h % ."MFBPCR"\AS.seek 0.579ml F*J' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*~'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +21 h F*n% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*-' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*B  +22 h  ."MFBPCR"\AS.seek 0ml F*_'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *{'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*I % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*6' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h % q ."MFBPCR"\AS.seek 0.605ml F* '\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*=' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*.  +21 h  ."MFBPCR"\AS.seek 0ml F*|'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *1'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*s  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *2' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*O n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *3'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*o'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*< n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *C' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*? I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*7  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *p': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*y  +65 h  F*e% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *W'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*b% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *H' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*8  +42 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *>': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*d  +64 h  F*}% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*m n +65 h n F*l% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*9  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*E n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *h'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*R n +150 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*a  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*5 % +43 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*N% h % ."MFBPCR"\AS.seek 0.97ml F*f'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*0 I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *P'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *i'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*x  +43 h  F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *t' +9988 h ' ."MFBPCR"\ATV.dial AIV F*M I +43 h I ."MFBPCR"\AS.seek 0.02ml F*!' +9880 h ' ."MFBPCR"\ATV.dial :waste F*k n +129 h n ."MFBPCR"\AS.seek 0ml F*@'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*A% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*K % +43 h % F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *('Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +21 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*T  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*'  +65 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *u'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*- % +87 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*{'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *3'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*` % +108 h % ."MFBPCR"\AS.seek 0ml F*<'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990939 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' '* h  P% %o h  F* *? h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ C' 2\ P% 0\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *C  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)7% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'%& S)#.0X'\ h ."rdySampler"\CSV.dial :waste1 C'm h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)l h  S)  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb % ' +20 h '\  ."closeHand"Hand.close M& / .t\Elbow.jog 1500 M&9* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'j'4 S)#.0X 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:43CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #24) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'N& h & .tHand.open M&1 +150 h 1 M&0& h &M M&f% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &Q(- +166 h (- M&P& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&L/ +150 h / M&x& h &- M&X% h %η .t\Forearm.seek :retract M&i'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &k' +91 h ' .t\CC.seek :closed C'A(W +416 h (W C'!& h &W .tHand.open M&@1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'z& h &W C'"% h %4 .t\CSV.dial CSR C'V  +150 h  C'F% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '('p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '&% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '#'[ +463 h '[ .t\CSV.dial :waste C'G +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'S'4 +2009 h '4 #t\Purging external Intake C'r& h &W C'% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  '4 h +75 .t\CTV.dial :intake C'u  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'J  +75 h #t\Flushing upper manifold C';& h &W C'n% h %4 .t\CSV.dial :flush C'~  +75 h  .t\CS.seek 5ml C'B'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +37 h @ C'_% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'/ +37 h .t\CS.seek 0ml C''4 +507 h '4 C'{& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C')'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'6% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'^'0 +507 h '0 .t\CSV.dial :waste C'. +75 h .t\CS.seek 0ml C'='4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +7 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&O' +91 h ' .tHand.close M&2/ +150 h / .t\CC.seek :open C' ( +416 h ( M&& h &ӷ M&`% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&q1 +150 h 1 M&D% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 11:45:48CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 3, 5 => 19, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:43CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*c% #tLoading sh1 puck from tube 5 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(*% h %X A(o. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (?(; +6 F*C% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *7' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (%& h &; M&$& h &- M&y% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &e(M +166 h (M M&p& h &M A(& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &b'x +90 h 'x A(W& h &; .t\SE.seek 17.71 Pucks below Top Plate A('* +86 h '* A(H& h &; M&]& h &M .t\Elbow.seek 5 M&8(M +7 h (M M&,& h &M A(d& h &; .t\Forearm.seek 5 + 300 counts M&}' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &m/ +150 h / ."downElevator"\SE.seek :down M&l& A('X h &M M&% h % .t\Forearm.seek :retract M& '\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&R(ӷ +142 h (ӷ M&9& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'\\(W +416 h (W .tHand.open M&U1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)5+ +7 h + S)% h % C'N& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +710 h ' .t\CSV.dial CSR C'  +150 h  C'N% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b 'Y' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +7 '?% h %  .t\CS.seek 4.5ml C'E'` +859 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'5  +38 h  .t\CS.seek 1.275ml C'f' +919 h ' #tHeating sh1 puck from tube 5 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'0   'P9 h 9d +100 C'\ 9 h 9d +100 C' 9 h 91d +100 C'79 h 9J d +100 C'9 h 9bd +100 C'x9 h 9zd +100 C'X9 h 9d +100 C'9 h 9d +100 C'L9 h 9 d +100 C'M9 h 9$d +100 C't9 h 9(d +100 C'Q9 h 9 ,d +100 C'i9 h 9$0d +100 C'9 h 9<4d +100 C'9 h 9S8d +100 C'9 h 9kd +100 C'n9 h 9Td +100 C'u9 h 9kd +100 C'~9 h 9d +100 C'9 h 9d +100 C'_9 h 9d +100 C'B9 h 9d +100 C'I9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'S9 h 9 d +100 C';9 h 96d +100 C'9 h 9Ld +100 C'{9 h 9bd +100 C'9 h 9xd +100 C'-9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'69 h 9d +100 C'+9 h 9d +100 C')9 h 9d +100 C' 9 h 9d +100 C'.9 h 9(d +100 C'|9 h 9>d +100 C'9 h 9Td +100 C'19 h 9id +100 C'/9 h 9d +100 C's9 h 9d +100 C'^9 h 9d +100 C'29 h 9d +100 C'K9 h 9d +100 C'A9 h 9d +100 C'`9 h 9  d +100 C'v9 h 9 d +100 C'[9 h 9 *d +100 C'O9 h 9 ?d +100 C'9 h 9 Td +100 C'q9 h 9 j d +100 C'9 h 9 $d +100 C'Z9 h 9 (d +100 C'39 h 9 ,d +100 C'c9 h 9 0d +100 C'9 h 9 4d +100 C'*9 h 9 8d +100 C'o9 h 9    P h  +30000  C'?9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'Y'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'5 @ +113 h @ .t\CSV.dial :air C'E @ +38 h @ .t\CS.seek 5ml C'P'p +1372 h 'p .t\CSV.dial CTV C'\  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'7  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'x'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'X +113 h .t\CBV.dial CSV C'  +19 h C'L% h %4 .t\CS.seek 5ml C'M'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C't  +37 h C'Q% h %p .t\CS.seek 7ml C'i'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C' @ +38 h @ .t\CS.seek 10ml C'' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C' ` +132 h ` #tLysing Complete C'z& h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M&"% h %\  A(V% h %X A(!. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (@(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (k& h &,9 M& & h &ӷ M&&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &((w +143 h (w M&G& h &w A(#& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &r' +89 h ' A(& h &,9 .t\SE.seek 1.71 Pucks below Top Plate A(T': +244 h ': M&F& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &4' +26 h '  .t\Elbow.hold M&J, +6 h ,w .t\Elbow.reconfigure TightElbow A('- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(=' +27 h ' M&n% h %  A(u& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&~'7 +20 h '7 M&& h &w A(_& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &B' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(I' +37 h ' M&& h &w M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +89 h '\  .t\Elbow.seek CC M&;(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&-/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &,9 A(6& h &,9 M&+& h &ӷ M&)% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &.(w +143 h (w M&|& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &1' +92 h '  .t\Elbow.hold M&/, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &s1 +150 h 1 M&^% h %  A(2& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&K'7 +21 h '7 M&A& h &w A(`& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &v' +22 h ' A([% h % ."downElevator"\SE.seek :down A(O'X +100 (% h %1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +42 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'Z @ + A(6 +18 h @ .t\CS.seek 10ml C'3' +6 h ' .t\CSV.dial between air and CTV C'c ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&o1 +2 h 1 M&C& h &w M&% h %\  .t\Elbow.seek FlushPuck::Garage M&D(- +166 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &- .t\Forearm.seek :garage M&$'η +91 h 'η .tHand.close M&/ +150 h / M&p& h &- M&% h %η .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'<(W +416 h (W C'H& h &W .tHand.open M&l1 +150 h 1 M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%d +151 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%e' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%: +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%9' +2610 h ' ."connect"\PRV.dial CBV P%}  .t\CBV.dial PRV C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  h .t\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial :air P%U  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%N' +2609 h ' .t\PSV.dial :air P%>  +38 h  .t\PS.seek 2.5ml P%?' +2610 h ' .t\PSV.dial :mixloop P%f +37 h .t\PS.seek 0ml P%5' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&E& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +7 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&7' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'P( +416 h ( M&X& h &ӷ M&% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &x(- +265 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &M'η +91 h 'η .tHand.open M&1 +150 h 1 M&0% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'& h & M&z% h %\  A("% h %X A(V. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (!(; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (k& h &; M& & h &- M&&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &G(M +166 h (M M&#& h &M A(& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &R'x +89 h 'x A(& h &; .t\SE.seek 16.71 Pucks below Top Plate A(F'; +96 h '; A(& h &; M&'& h &M .t\Elbow.seek 5 M&n(M +6 h (M M&u& h &M A(& h &; .t\Forearm.seek 5 + 300 counts M&_' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&r& A('X h &M M&(% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.seek CC M&4(ӷ +4 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' .t\CC.seek :closed C'=(W +416 h (W .tHand.open M&B1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +150 h  .t\CSV.dial CTV C'\\  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'6' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'+'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%)  +37 h  .t\PS.seek 2.5ml P%|' +2610 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%; +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%s  +151 h  .t\PS.seek :empty P%^' +2609 h ' .t\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'A  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'` @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'[  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'. @ +37 h @ .t\CS.seek 10ml C'' +2648 h ' .t\CSV.dial CTV C'q  +37 h  .t\CS.seek 0ml C'Z'4 +2647 h '4 .t\CBV.dial :waste1 C'1 @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'c  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&*% h %\  A(o% h %X A(C. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (K& h &,9 M&I& h &ӷ M&v% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &a(w +143 h (w M&%& h &w A(@& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &O' +89 h ' A(& h &,9 .t\SE.seek 2.71 Pucks below Top Plate A(/') +235 h ') M&& h &w A(p& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &' +25 h '  .t\Elbow.hold M&, +7 h ,w .t\Elbow.reconfigure TightElbow A($- h -+) M&$&.,(\ #b .t\SE.seek 3.04 Pucks below Top Plate A(' +26 h ' M&]% h %  A(D& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&W'7 +21 h '7 M&& h &w A(H& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &l' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(g' +36 h ' M&d& h &w M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&b(ӷ +143 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&}/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &,9 A( & h &,9 M&9& h &ӷ M&U% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +142 h (w M& & h &w A(>& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &N' +93 h '  .t\Elbow.hold M&f, +6 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &?1 +150 h 1 M&E% h %  A(\ & h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M& '7 +21 h '7 M&& h &w A(y& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &m' +22 h ' A(<% h % ."downElevator"\SE.seek :down A(X'X +100 (% h %  M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  +31 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%Q  +2 A(6 +111 h  P%Y% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%0 +38 h .t\PRV.dial :air P%L  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'z  +38 h .t\PRV.dial between CBV and air P%"  +18 h  .t\PRV.dial :air P%V  +19 h  P%k% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +251 h ' .t\PRV.dial :waste2 P%& @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %M' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'#& h & .tHand.open M&1 +2 h 1 M&& h &w M&x% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&i/ +150 h / M&& h &- M&r% h %η .t\Forearm.seek :retract M&('\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +265 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &{' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'_& h &W .tHand.open M&n1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  C'P& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%B @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%J  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  .tdelay 2 seconds +200 P%\\% h % .t\PS.seek 1.499ml P%~' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%!' +1094 h ' .t\PSV.dial :air P%3  +37 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%6 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%)  +37 h  .tdelay 2 seconds +200 P%5% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%;% h %r .t\PS.seek 0.475ml P%s' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%|  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%A  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%`  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %[' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%. @ +75 h @ .t\PS.seek 0ml P%+' +2610 h ' .t\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P%' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%1 +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%c' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%*' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%o +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%C  +76 h P%K% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %I' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'v & +50 h & .t\AWV.select :waste C' +6 P%%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %@'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %4' +1312 h ' .t\PSV.dial :air P%-  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%p +37 h .t\PS.seek 0ml P%$' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 ']& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'D& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T *H  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%d +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*a I +129 h I F*/% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%e  +37 h  .t\PS.seek 0ml P%:' +1598 h ' .t\PSV.dial PSR P%W +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%l +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*}  +22 h ' .t\PSV.dial PSR P%, +21 h  F*=% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%9' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*U % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*b  +22 h  F*?% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *E' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%\ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +217 ."MFBPCR"\ATV.dial :AIV F*y I +43 h I F*<% h % ."MFBPCR"\AS.seek 0.469ml F*8' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%7 +113 h P%N% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%g  +38 h  .t\PS.seek 0ml P%Q' +739 h ' ."MFBPCR"delay 5 seconds +500 F*Y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*m n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%0' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%L +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%z +113 h P%"% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%V' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%k  +37 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%& +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%x  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %'' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*u I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *f'V +849 h ' .t\PSV.dial :air P%i  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%r +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %t' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %R' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *('Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*_% h %Q ."MFBPCR"\AS.seek 0.01ml F*n' +170 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %P' +618 h ' ."MFBPCR"\ATV.dial :waste F*B n +129 h n ."MFBPCR"\AS.seek :empty F*{'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*J  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *\\' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*M  +43 h F*~% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *3'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'!  +37 h  C'6% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C')'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'5' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'; @ +37 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*|  +22 h ."MFBPCR"delay 3 seconds +300 F*s% h %_ ."MFBPCR"\AS.seek 0.434ml F*A'ݏ +110 h '4 .t\CSV.dial CSR C'`  +37 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C' 'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*.  +22 h F*[% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*q'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*+ n +107 h n F*1% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'o'4 +2647 h '4 C'C& h &W #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +7 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&I' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'@( +416 h ( M&-& h &ӷ M&4% h % .t\Forearm.seek :retract M&p'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &H'η +91 h 'η .tHand.open M&d1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'a& h & M&/% h %\  A(e% h %X A(j. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (O(; +200 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (W& h &; M&:& h &- M&l% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &(M +166 h (M M&}& h &M A(=& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &,'x +89 h 'x A( & h &; ."WCR"\SE.seek 15.71 Pucks below Top Plate A('L +107 h 'L A(& h &; M&U& h &M ."WCR"\Elbow.seek 5 M&(M +6 h (M M&>& h &M A(b& h &; ."WCR"\Forearm.seek 5 + 300 counts M&?' +23 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &9/ +147 h '&0 ."MFBPCR"delay 3 seconds +3 h / ."downElevator"\SE.seek :down M&\ & h &M A( 'X M&y% h % ."WCR"\Forearm.seek :retract M&<'\ +91 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +15 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&E& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +64 ."MFBPCR"\ATV.dial :waste2 F*7 % +27 h ' ."WCR"\CC.seek :closed C'N(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +400 h (W ."WCR"Hand.open M&g1 +150 h 1 M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +10 h 'R ."MFBPCR"\ATV.dial :air2 F*m  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *X' +59 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)8+ +6 h + S)^% h % C'Q& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'L  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*b n +86 h n ."MFBPCR"\ASV.dial :wasteR F*$  +43 h  ."MFBPCR"\AS.seek 0ml F*9'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *y'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +108 h % F*,% h %V ."MFBPCR"\AS.seek 0.59ml F*E' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*?% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*7  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F* % h % ."MFBPCR"\AS.seek 0.12ml F*<' +626 h ' ."MFBPCR"\ATV.dial :air3 F*@ % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.23ml F*^' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*O% h %X ."MFBPCR"\AS.seek 0.24ml F*8' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*" n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*z  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *V'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*0 @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*&'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*x  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *u'\  +1032 h '\  F*#% h %\  ."MFBPCR"\AS.seek 0.5ml F*i'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*t'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *'' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*r % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2417h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*_  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*(% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*n  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*M  +22 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *{'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*\\  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*6% h %Fj ."MFBPCR"\AS.seek 0.524ml F*!' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*)  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*Z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*; % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +107 h  ."MFBPCR"delay 7 seconds +700 F*`% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*| n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*[  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.65ml F*+' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*A  +43 h  ."MFBPCR"\ASV.dial :waste1 F*q  +65 h ."MFBPCR"\ARV.dial :air1 F*1  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*c% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*K % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226068 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'v  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'C @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'G @ +75 h @ +50 S)%% h % ."WCR"\CBV.dial between CSV and waste2b C'F  +95 h  C'-% h % ."WCR"\CSV.dial between air and CTV C'T ` +18 h ` ."WCR"\CS.seek 7.288ml C'I' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C']  '4  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '*'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'd  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'D' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'p  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +37 h  C'/% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'j' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'a @ +75 h @ +50 S)e% h % ."WCR"\CBV.dial between CSV and waste2b C'l  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C': ` +56 h ` ."WCR"\CS.seek 7.288ml C'=' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '}  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'U  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'b  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'$  +38 h  C'>% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'9' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '\ ' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'y  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'? ` +57 h ` ."WCR"\CS.seek 7.288ml C'E' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'7  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '@' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'<& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M& % h %\  A(m% h %X A(g. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (Q(,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (L& h &,9 M&^& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &O(w +142 h (w M&& h &w A("& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &z' +89 h ' A(8& h &,9 ."WCR"\SE.seek 3.71 Pucks below Top Plate A(k' +224 h ' M&& h &w A(V& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &' +25 h '  ."WCR"\Elbow.hold M&, +7 h ,w ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(x' +27 h ' M&% h %  A(&& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts + M&#'7 +21 h '7 M&u& h &w A( & h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &i' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(' +37 h ' M&& h &w M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  ."WCR"\Elbow.seek CC M&r(ӷ +142 h (ӷ M&'& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&_' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'(( +416 h ( A(f& h &,9 A(& h &,9 M&X& h &ӷ M&B% h % ."WCR"\Forearm.seek :retract M&J'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &n(w +143 h (w M&M& h &w A(~& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &S' +92 h '  ."WCR"\Elbow.hold M&\\, +7 h ,w ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &61 +150 h 1 M&{% h %  A()& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts + M&'7 +21 h '7 M&!& h &w A(;& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &5' +22 h ' A(Z% h % ."downElevator"\SE.seek :down A(s'X +100 (`% h % M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +90 h '\  +21 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&21 +2 A(6 h 1 M&[& h &w M&.% h %\  .t\Elbow.seek FlushPuck::Garage M&3(- +166 h (- M&A& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.seek :garage M&1'η +91 h 'η .tHand.close M&c/ +150 h / M&+& h &- M& % h %η .t\Forearm.seek :retract M&K'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +265 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &G' +91 h ' .t\CC.seek :closed C'%(W +415 h (W C'F& h &W .tHand.open M&-1 +150 h 1 M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'4& h &W C']% h % .t\CSV.dial CSR C'I  +38 h  C'd% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'*'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'p% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'/ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'j& h &W C'a% h %4 .t\CSV.dial :flush C'e  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'l'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C':% h %p .t\CS.seek 7ml C'W'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '}  +37 h  +113  .t\CBV.dial :waste1 C'= @ +75 h @ .t\CS.seek 0ml C'U'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'b% h %4 .t\CSV.dial :flush C'$  +76 h  .t\CS.seek 5ml C'>'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'9% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C',  +37 h  .t\CBV.dial :waste2b C'y  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #t\Flushing upper manifold C'?& h &W C'\ % h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '<  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'L +37 h .t\CS.seek 0ml C'^'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 3ml C'8'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'Y% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'V'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'&'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'u& h &W C' % h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'z'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'O' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C''' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'x  +113 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C't  'N  +37 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'X  +37 h  C'B% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'M'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'_  +38 h  .t\CBV.dial :waste2b C'r  +75 h  .t\CS.seek 0ml C'6'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'{  +38 h  C')% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '!'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ';' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Z 'J  +38 h +75 .t\CTV.dial :air C'n  +151 h .t\CS.seek 0ml C'`'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'2'4 +506 h '4 #t\Flushing upper manifold C'[& h &W C'.% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'A% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV C'5 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +506 h '0 .t\CSV.dial :waste C'\\ +38 h .t\CS.seek 0ml C'c'4 +506 h '4 C'+& h &W C' % h %4 .t\CSV.dial :flush C'|  +38 h  .t\CS.seek 3ml C'1'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +38 h @ C'o% h %0 .t\CS.seek 5ml C'3'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'K'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +507 h '0 .t\CSV.dial :waste C'F +75 h .t\CS.seek 0ml C'-'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'T& h &W C'4% h %4 .t\CSV.dial :flush C']  +37 h  .t\CS.seek 5ml C'I'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +37 h @ C'G% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'p  '*  +38 h  +113  .t\CBV.dial :waste1 C'/ @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'j& h &W C'a% h %4 .t\CSV.dial :flush C'e  +75 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'H @ +38 h @ C':% h %p .t\CS.seek 7ml C'l'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'}  +76 h  .t\CS.seek 0ml C'='4 +766 h '4 #tDisinfecting external Intake with kill C'W& h &W C'% h %4 .t\CSV.dial CSR C'b  +37 h  C'$% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'U'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'> '9  +37 h +76 .t\CTV.dial :intake C',  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'y' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '?'4 +607 h '4 .t\CSV.dial CSR C'\  +112 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'E' +1373 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'7'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&m' +91 h ' .tHand.close M&L/ +150 h / .t\CC.seek :open C'g( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&"'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &^(- +264 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&1 +150 h 1 M&V% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&u1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &- .t\Forearm.seek :garage M&z'η +91 h 'η .tHand.close M&k/ +150 h / M&& h &- M&O% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&x& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &#' +91 h ' .t\CC.seek :closed C'(W +416 h (W C't& h &W .tHand.open M&N1 +150 h 1 M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'X& h &W C'B% h %4 .t\CSV.dial CSR C'  +113 h  C'M% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'_'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '~'[ +463 h '[ .t\CSV.dial :waste C'{ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ')'4 +2010 h '4 #t\Purging external Intake C'6& h &W C'!% h %4 .t\CSV.dial :flush C'J  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'n .t\CBV.dial CSV +75 h .t\CTV.dial :intake C';  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'`'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'[% h %4 .t\CSV.dial :flush C'.  +76 h  .t\CS.seek 5ml C'S'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'5'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +506 h '0 .t\CSV.dial :waste C's +38 h .t\CS.seek 0ml C'\\'4 +506 h '4 C'Q& h &W C'+% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'|'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +37 h @ C'C% h %0 .t\CS.seek 5ml C'o'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'3'0 +507 h '0 .t\CSV.dial :waste C'K +75 h .t\CS.seek 0ml C'F'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&(& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &T(ӷ +6 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.seek CC M&-' +91 h ' .tHand.close M&d/ +150 h / .t\CC.seek :open C'G( +416 h ( M&I& h &ӷ M&p% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +265 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &a'η +91 h 'η .tHand.open M&e1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +90 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until 14PM #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14PM #\Continuing at 14:00CEST25-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 5: mbaPhase\\n/home/esp/esp2/mission/18tundeployv2.rb:135\\n{1 => 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:47:31CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST25-Oct-18" +263668 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2417h1500ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*$'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*9'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*,% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*y  +64 h  F*\ % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*L % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2419h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ARV.dial :carrier F*x  +11 h  F*i% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*g n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*X  +22 h ."MFBPCR"delay 2 seconds +200 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*M  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*7' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*r  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*_% h % ."MFBPCR"\AS.seek 0.53ml F*{' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*~  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*J  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*n'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*; n +21 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*`  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*A % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2419h1500ml.pcr F*5% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*q  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*c% h %Z ."MFBPCR"\AS.seek 0.81ml F*C'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*3% h %n ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*4  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +65 h  F*d% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*j  +32 h  ."MFBPCR"\ATV.dial :air3 F*T % +87 h % F*e% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +22 h F*a% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*l% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2421h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*L  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*v  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *%'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*h I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.536ml F*"'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*&'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*8  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*^  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*x'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2421h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *X'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +21 h F*N% h %Z ."MFBPCR"\AS.seek 0.81ml F*M'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +22 h F*7% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*6  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*` % +87 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*5% h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*+'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V @CEST1540418421.90 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2500h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ARV.dial :carrier F*(  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*j  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *@'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F** I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*: n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*}  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*W n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*9  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*y% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2500h1500ml.pcr F*\ % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*E  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *L'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*%'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*h  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*m'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Y  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *V'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F*& % +86 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*x'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*g  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *B'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*N' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2502h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +10 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*!'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *n'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*`  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *['Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*. I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*0 % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*5% h % ."MFBPCR"\AS.seek 0.53ml F*A' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*q  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*S'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*s  +21 h  ."MFBPCR"delay 7 seconds +700 F*+% h %< ."MFBPCR"\AS.seek 0.594ml F*\\'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*c n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*|'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*C% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*3  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*1  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*4% h % \\ ."MFBPCR"\AS.seek 0.326ml F*d'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2502h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*] @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *j' +424 h ' ."MFBPCR"\ATV.dial :ASV F*T n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*-% h % ."MFBPCR"\AS.seek 0.62ml F**'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*a n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *@'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *:'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +42 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *l' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +22 h F* % h % ."MFBPCR"\AS.seek 0.71ml F*W' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h % H ."MFBPCR"\AS.seek 0.735ml F*$' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*U% h % Ű ."MFBPCR"\AS.seek 0.72ml F*9' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*y% h % zG ."MFBPCR"\AS.seek 0.365ml F*,' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*\ % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *E' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*L'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*h n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*%'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Y'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*m% h %V ."MFBPCR"\AS.seek 0.01ml F*V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*"% h % ."MFBPCR"\AS.seek 0.58ml F* ' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h % q ."MFBPCR"\AS.seek 0.605ml F*k'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*&  +22 h F*O% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*8' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*z n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*^'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*i  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *x'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*g n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +202 h 'R ."MFBPCR"\SPE.coast F*B  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*X n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*#  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *M' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*_ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*6  +43 h  F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *~': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*)  +65 h  F*{% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *!'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*Z n +65 h n F*;% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *n' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*[  +65 h  F*0% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *5'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Q' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*+  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *S': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*c n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b * 'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*\\ n +151 h n ."MFBPCR"\AS.seek 0ml F*o'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*C  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*| % +42 h % F*3% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *1' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*K% h % ."MFBPCR"\AS.seek 0.96ml F*('0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*d' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*p% h % ."MFBPCR"\AS.seek 0.97ml F*I'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*T I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *-'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *a'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*:  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *}' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*l' +9880 h ' ."MFBPCR"\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.seek 0ml F* 'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *='(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*b  +22 h ."MFBPCR"\ATV.dial :air3 F*W % +43 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*$  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*,'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*?'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*E% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *v'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*%% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*Y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*V'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*u% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *O'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*B n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *x'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*f % +107 h % ."MFBPCR"\AS.seek 0ml F*X'V +2548 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" +2990137 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )# h  C' ' h  P% %N h  F* *_ h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *M  h  .t\SPE.configure SPEconfig F*\ 2 *7% h %V .t\AS.configure ASconfig S)6% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'J& S)#.0X 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:30CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #25) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'p& h & .tHand.open M&d1 +150 h 1 M&T& h &M M&I% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &e(- +166 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &a'η +91 h 'η .tHand.close M&*/ +150 h / M&:& h &- M&% h %η .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &D' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'l& h &W .tHand.open M& 1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'>& h &W C'=% h %4 .t\CSV.dial CSR C'$  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'U'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '9% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '\ '4 +2009 h '4 #t\Purging external Intake C'& h &W C',% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '<' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'E '?  h +75 .t\CTV.dial :intake C'v  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'L  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'%% h %p .t\CS.seek 7ml C'h'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'm'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'"'4 +507 h '4 C'Y& h &W C'% h %4 .t\CSV.dial :flush C'u  +38 h  .t\CS.seek 3ml C'V'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +38 h @ C' % h %0 .t\CS.seek 5ml C'O'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'k'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'8'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&^& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'B( +416 h ( M&g& h &ӷ M&f% h % .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&X1 +150 h 1 M&P% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 5 at 11:48:36CEST30-Sep-18\\n{1 => 22, 2 => 22, 3 => 22, 4 => 6, 5 => 16, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:30CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*H% #tLoading sh1 puck from tube 5 into Collection Clamp h %&K C']& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&#% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(N. h ., .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (_(; +6 F*M% h %&K ."readyMFB"\ATV.dial :waste2 F*7 % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *6' +508 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (J& h &; M&r& h &- M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &{(M +166 h (M M&~& h &M A(Z& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &;'x +90 h 'x A(!& h &; .t\SE.seek 14.71 Pucks below Top Plate A('] +116 h '] A(n& h &; M&`& h &M .t\Elbow.seek 5 M&(M +6 h (M M&.& h &M A(0& h &; .t\Forearm.seek 5 + 300 counts M&[' +22 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(q'X M&2& h &M M&5% h % .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.seek CC M&\\(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +118 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &1' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&'1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)|+ +6 h + S)o% h % C'p& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X& h &W E ;3\\0\\0 .t\CTV.dial :intake ."connect"\SSV.dial CBV .t\CBV.dial SSV S)=  +75 h  #t\Allowing 130s for 25.0ml of remaining 25.0ml .Intake.select :open S) +206 C'$% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9@d +100 C'=9 h 9Dd +100 C'$9 h 9Hd +100 C'l9 h 9Ld +100 C' 9 h 9Pd +100 C'D9 h 9Td +100 C'A9 h 9Xd +100 C'99 h 9(\\d +100 C'U9 h 9@`d +100 C'y9 h 9Wdd +100 C'9 h 9nhd +100 C'9 h 9ld +100 C',9 h 9pd +100 C'9 h 9td +100 C'9 h 9xd +100 C'?9 h 9|d +100 C'E9 h 9d +100 C'9 h 9d +100 C'<9 h 9'd +100 C'89 h 9>d +100 C'9 h 9Td +100 C'v9 h 9kd +100 C'L9 h 9d +100 C' 9 h 9d +100 C'%9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'h9 h 9d +100 C'm9 h 9 d +100 C'\ 9 h 9 d +100 C'9 h 96d +100 C'9 h 9Ld +100 C'Y9 h 9bd +100 C'9 h 9xd +100 C' 9 h 9d +100 C'"9 h 9d +100 C'&9 h 9d +100 C' 9 h 9d +100 C'u9 h 9d +100 C'V9 h 9d +100 C'k9 h 9d +100 C'9 h 9(d +100 C'^9 h 9>d +100 C'O9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'i9 h 9d +100 C'z9 h 9d +100 C'9 h 9d +100 C'W9 h 9d +100 C'9 h 9d +100 C'f9 h 9  d +100 C't9 h 9 d +100 C'}9 h 9 *d +100 C'/9 h 9 ?d +100 C'F9 h 9 Td +100 C'X9 h 9 j d +100 C'g9 h 9 $d +100 C'9 h 9 (d +100 C'x9 h 9 ,d +100 C'H9 h 9 0d +100 C'#9 h 9 4d +100 C'9 h 9 8d +100 C'N9 h 9 & h &W #t\Storing sh1 puck in Collection Clamp to tube 4 M&=% h %\  A($% h %X A(l. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ( (,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &,9 M&A& h &ӷ M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &U(w +143 h (w M&y& h &w A(& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(,& h &,9 .t\SE.seek 4.71 Pucks below Top Plate A(' +214 h ' M&& h &w A(?& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &E' +26 h '  .t\Elbow.hold M&, +6 h ,w .t\Elbow.reconfigure TightElbow A(<- M&$&.,(\ #b h -+ .t\SE.seek 5.04 Pucks below Top Plate A(8' +27 h ' M&% h %  A(v& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts +2 M&L'7 +20 h '7 M& & h &w A(%& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 6.04 Pucks below Top Plate A(' +37 h ' M&h& h &w M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'"( +416 h ( A(&& h &,9 A( & h &,9 M&u& h &ӷ M&V% h % .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +143 h (w M&^& h &w A(O& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &' +92 h '  .t\Elbow.hold M&, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&z% h %  A(& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&W'7 +21 h '7 M&& h &w A(f& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &t' +22 h ' A(}% h % ."downElevator"\SE.seek :down A(/'X +100 (F% h % M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &g'\ +90 h '\  +12 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'x' +7 h ' .t\CSV.dial between air and CTV C'H ` +18 h ` .t\AIV.dial :bypass C'# { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&N1 +2 h 1 M&M& h &w M&7% h %\  .t\Elbow.seek FlushPuck::Garage M&P(- +166 h (- M&C& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.seek :garage M&r'η +91 h 'η .tHand.close M&]/ +150 h / M&~& h &- M&Z% h %η .t\Forearm.seek :retract M&!'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +265 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +90 h ' .t\CC.seek :closed C'G(W +416 h (W C'n& h &W .tHand.open M&21 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%0 +151 h P%5% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +38 h  .t\PS.seek 0ml P% ' +1597 h ' .t\PSV.dial PSR P%4 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%+' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%[  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%Q  h .t\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial :air P%'  +38 h  .t\PS.seek 2.5ml P%B' +2609 h ' .t\PSV.dial :mixloop P%o +38 h .t\PS.seek 0ml P%p' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%_' +2610 h ' .t\PSV.dial :mixloop P%I +38 h .t\PS.seek 0ml P%|' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&c& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &1(ӷ +7 h (ӷ M&j& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&3' +91 h ' .tHand.close M&d/ +150 h / .t\CC.seek :open C'-( +416 h ( M&& h &ӷ M&K% h % .t\Forearm.seek :retract M&*'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &:(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&(1 +150 h 1 M&T% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 5 into Collection Clamp C'b& h & M&>% h %\  A(=% h %X A($. h . .t\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (l(; +200 h (; .t\SC.reconfigure SCloose A($.(\ 1~b (D& h &; M&A& h &- M&9% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &y(M +166 h (M M&& h &M A(,& h &; .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &\\'x +90 h 'x A(S& h &; .t\SE.seek 13.71 Pucks below Top Plate A('n +125 h 'n A(?& h &; M&<& h &M .t\Elbow.seek 5 M&(M +6 h (M M&v& h &M A( & h &; .t\Forearm.seek 5 + 300 counts M&%' +23 h ' .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &h/ +150 h / ."downElevator"\SE.seek :down A(m'X M&& h &M M&U% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&E(ӷ +34 h 'X ."downElevator"\SE.coast A(5 +108 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C'8(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'\  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'&'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C' ' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'u'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%V  +37 h  .t\PS.seek 2.5ml P%^' +2610 h ' .t\PSV.dial :mixloop P%O +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%i  +151 h  .t\PS.seek :empty P%z' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'f @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'}  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'k ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'F' +2648 h ' .t\CSV.dial CTV C'X  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C'H @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'#  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 4 M&N% h %\  A(M% h %X A(7. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (W& h &,9 M&& h &ӷ M&t% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &C(w +143 h (w M&J& h &w A( & h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &/' +89 h ' A(a& h &,9 .t\SE.seek 5.71 Pucks below Top Plate A(' +205 h ' M&]& h &w A(~& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 4 M&#Q@2^d^2b &Z' +25 h '  .t\Elbow.hold M&g, +7 h ,w .t\Elbow.reconfigure TightElbow A(r- M&$&.,(\ #b h -+ .t\SE.seek 6.04 Pucks below Top Plate A(w' +27 h ' M&`% h %  A(P& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&!'7 +21 h '7 M&"& h &w A(n& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &2' +22 h ' .t\SE.seek 7.04 Pucks below Top Plate A(R' +36 h ' M&0& h &w M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +90 h '\  .t\Elbow.seek CC M&;(ӷ +142 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h &,9 A(Q& h &,9 M&+& h &ӷ M&'% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &o(w +143 h (w M&B& h &w A(& h &,9 .t\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &p' +92 h '  .t\Elbow.hold M&I, +7 h ,w .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &_1 +150 h 1 M&c% h %  A(j& h &,9 .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 4 + 300 counts + M&6'7 +21 h '7 M&d& h &w A()& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(G% h % ."downElevator"\SE.seek :down A('X +100 (K% h % M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +2 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%e  +2 A(6 +111 h  P%1% h % .t\PS.seek 0.499ml P%(' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%T +37 h .t\PRV.dial :air P%*  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%b  +38 h .t\CBV.dial AIV C'>  +37 h .t\PRV.dial between CBV and air P%=  +19 h  .t\PRV.dial :air P%$  +19 h  P%D% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %A' +250 h ' .t\PRV.dial :waste2 P%9 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&,1 +2 h 1 M&S& h &w M&:% h %\  .t\Elbow.seek FlushPuck::Garage M&?(- +166 h (- M&<& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&@/ +150 h / M&h& h &- M&% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'%(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'-& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%\  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%L' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%l' +1094 h ' .t\PSV.dial :air P%x  +38 h  .t\PS.seek 0ml P%&' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%V  +38 h  .tdelay 2 seconds +200 P%|% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %O'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%i' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%^  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%f  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %}' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%k +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%u' +2610 h ' .t\PSV.dial :air P%X  +75 h  .t\PS.seek 2.5ml P%F' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%H +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%#' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%N' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%M +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%7  +75 h P%W% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C't & +50 h & .t\AWV.select :waste C' +6 P%J% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'a { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %E' +1313 h ' .t\PSV.dial :air P%]  +38 h  .t\PS.seek 2.5ml P%~' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%r' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '`& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'P& ."MFBPCR"\ASV.dial :SPEdelivery F*n  h &W F*" @@T .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%0 +22 h  ."MFBPCR"delay 20 seconds +91 h P%5% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%/' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*C I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +65 .t\PSV.dial :waste1 P%{  +38 h  .t\PS.seek 0ml P%4' +1597 h ' .t\PSV.dial PSR P%! +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%w' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%2 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%g' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*[  +22 h ' .t\PSV.dial PSR P%. +20 h  F*8% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +93 h P%Q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%+' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*' % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P% ' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*;  +22 h  F*_% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *c' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%j +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%d' +217 ."MFBPCR"\ATV.dial :AIV F*) I +43 h I F*G% h % ."MFBPCR"\AS.seek 0.469ml F*q' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%o +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +2609 h ' .t\PSV.dial PSR P%3 +113 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%R  +37 h  .t\PS.seek 0ml P%e' +740 h ' ."MFBPCR"delay 5 seconds +500 F*19 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*'% +337 h ' .t\PSV.dial PSR P%z +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%T' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%* +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%b' +2610 h ' .t\PSV.dial PSR P%> +113 h P%=% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%D  +38 h  .t\PS.seek 0ml P%(' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%9 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%A' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %S'k +2678 h 'k .t\PSV.dial :waste1 P%:  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %<' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*v I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *I'V +850 h ' .t\PSV.dial :air P%@  +113 h  .t\PS.seek 2.5ml P%h' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\\' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *U'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%m +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %-' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*Y'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*\ % h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*L% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *x'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'l  +38 h  C' % h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'V'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'|' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'&  '  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'O'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*^  +21 h ."MFBPCR"delay 3 seconds +300 F*i% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'f  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'k'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F*}% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*X'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*u n +108 h n F*H% h %& ."MFBPCR"\AS.seek 0.092ml F*F'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'#  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'M'4 +2647 h '4 C'7& h &W #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&a/ +150 h / .t\CC.seek :open C' ( +416 h ( M&]& h &ӷ M&E% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &~(- +264 h (- M&`& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &n'η +91 h 'η .tHand.open M&01 +150 h 1 M&5% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 5 into Collection Clamp C'C& h & M&% h %\  A({% h %X A(s. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 5 A($\ .(\ 1~b (/(; +201 h (; ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (!& h &; M&4& h &- M&2% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 5 M&$&.,(\ #b &w(M +166 h (M M&[& h &M A(8& h &; ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 700 counts M&#Q@2^d^\ b &.'x +89 h 'x A(Q& h &; ."WCR"\SE.seek 12.71 Pucks below Top Plate A(Z' +136 h ' A(g& h &; M&'& h &M ."WCR"\Elbow.seek 5 M&(M +7 h (M M&& h &M A(;& h &; ."WCR"\Forearm.seek 5 + 300 counts M&_' +22 h ' ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down A(B'X M&j& h &M M&)% h % ."WCR"\Forearm.seek :retract M&G'\ +91 h '\  ."WCR"\Elbow.seek CC M& (ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&c& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &d' +35 ."MFBPCR"\ATV.dial :waste2 F*3 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +13 h ' ."WCR"\CC.seek :closed C'K(W +416 h (W ."WCR"Hand.open M&R1 +131 h 'R ."MFBPCR"\ATV.dial :air2 F*6  +19 h 1 M&1% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +89 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)q+ +7 h + S)z% h % C'e& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'*  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  ."WCR"\CBV.dial SSV C'= @ +151 h @  ."WCR"delay 3.8 seconds +380 C'b% h %4 S)D% h %5J ."WCR"\SSV.dial :spit S)$ +150 h ."WCR"\SS.seek 0ml S)9' +216 h ' ."WCR"\SSV.dial CBV S)(  +151 h  ."WCR"\CTV.dial :intake C'  +75 h  ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +207 C',% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *$'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*T @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*,'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*9'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*:  +43 h  #"MFBPCR"\Swish #1 of 2 F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *v'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*@'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*A% h %(A ."MFBPCR"\AS.seek 0.6ml F*h'\  +1031 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *<' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2517h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*L% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*V  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.536ml F*&'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.6ml F*x'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*}  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*X  +64 h ."MFBPCR"\ARV.dial :air1 F*H  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*#% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*k  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226098 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C't  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'7 @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'a' +909 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S)J% h % ."WCR"\CBV.dial between CSV and waste2b C'%  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'`  'E  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'N'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'0  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'P' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'5  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C's' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'C @ +75 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'2  +94 h  C'n% h % ."WCR"\CSV.dial between air and CTV C'4 ` +57 h ` ."WCR"\CS.seek 7.288ml C'8' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Q  '[  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'g'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C''  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '~' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C';  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'r  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'+' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +816 h ' #"WCR"\Pumping wcr puck from tube 5 up to 55psi... .\CBV.dial SSV C'w @ +75 h @ +50 S).% h % ."WCR"\CBV.dial between CSV and waste2b C')  +95 h  C'o% h % ."WCR"\CSV.dial between air and CTV C'Z ` +56 h ` ."WCR"\CS.seek 7.288ml C'c' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'3  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'B'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'_  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'F' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'G& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 4 M&1% h %\  A(6% h %X A(R. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (e(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (*& h &,9 M&z& h &ӷ M&p% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &/(w +142 h (w M&d& h &w A(=& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &>' +90 h ' A(q& h &,9 ."WCR"\SE.seek 6.71 Pucks below Top Plate A(D' +195 h ' M&b& h &w A($& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 4 M&#Q@2^d^2b &' +26 h '  ."WCR"\Elbow.hold M&(, +6 h ,w ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(,- h -+ ."WCR"\SE.seek 7.04 Pucks below Top Plate A(:' +27 h ' M&S% h %  A(9& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&'7 +20 h '7 M&v& h &w A(A& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &@' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A( 'w +37 h 'w M&h& h &w M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&<& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&m/ +150 h / ."WCR"\CC.seek :open C'U( +415 h ( A(I& h &,9 A(?& h &,9 M&& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +143 h (w M&& h &w A(L& h &,9 ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 4 M&$ ,(#b &\ ' +92 h '  ."WCR"\Elbow.hold M&, +7 h ,w ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&Y% h %  A(V& h &,9 ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 4 + 300 counts +2 M&'7 +20 h '7 M&l& h &w A(& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &|' +22 h ' A(% h %w ."downElevator"\SE.seek :down A(i'X +100 (f% h % M&&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast A(6 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'O& h & .tHand.open M&1 +2 h 1 M&}& h &w M&% h %\  .t\Elbow.seek FlushPuck::Garage M&x(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&H'η +91 h 'η .tHand.close M&#/ +150 h / M&u& h &- M&k% h %η .t\Forearm.seek :retract M&W'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &y' +91 h ' .t\CC.seek :closed C'J(W +416 h (W C'%& h &W .tHand.open M&]1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'E& h &W C'`% h % .t\CSV.dial CSR C'  +37 h  C'0% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'N'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '5'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'P'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C's& h &W C'C% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '2'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +38 h @ C'4% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'[  'Q  +38 h  +112  .t\CBV.dial :waste1 C'8 @ +75 h @ .t\CS.seek 0ml C'''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'g& h &W C';% h %4 .t\CSV.dial :flush C'r  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +38 h @ C'+% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV C'.  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C')  +75 h  .t\CS.seek 0ml C'o'4 +766 h '4 #t\Flushing upper manifold C'Z& h &W C'j% h %4 .t\CSV.dial :flush C'3  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +38 h @ C'_% h %p .t\CS.seek 7ml C'B'i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'G  +38 h +75 .t\CTV.dial :air C'1  +151 h .t\CS.seek 0ml C'6'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'R'0 +507 h '0 .t\CSV.dial :waste C'* +38 h .t\CS.seek 0ml C'z'4 +506 h '4 C'p& h &W C'd% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.seek 3ml C'q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %0 .t\CS.seek 5ml C'$'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +507 h '0 .t\CSV.dial :waste C'S +75 h .t\CS.seek 0ml C'9'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'v& h &W C'A% h %4 .t\CSV.dial CSR C'h  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'>'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'/' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'D +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'<' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C':  +113 h  C'@% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'('P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'm' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'K  +38 h  +113  .t\CBV.dial :waste1 C'I @ +37 h @ .t\CS.seek 0ml C'?'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C' '4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'Y  +38 h  C'V% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'l'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'f'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +506 h '0 .t\CSV.dial :waste C'O +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'}& h &W C'% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'| +113 h .t\CTV.dial :air .\CS.seek 0ml C'i'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'e'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'#'4 +507 h '4 C'u& h &W C'k% h %4 .t\CSV.dial :flush C'^  +37 h  .t\CS.seek 3ml C'H'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +37 h @ C'M% h %0 .t\CS.seek 5ml C'x'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'W'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +506 h '0 .t\CSV.dial :waste C'% +76 h .t\CS.seek 0ml C']'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'E% h %4 .t\CSV.dial :flush C'`  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'y% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'N  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'5'4 +766 h '4 #t\Flushing lower manifold's waste2b port C's& h &W C'C% h %4 .t\CSV.dial :flush C'{  +75 h  .t\CS.seek 5ml C'P'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'n @ +38 h @ C'4% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CTV C'[  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'Q  +75 h  .t\CS.seek 0ml C'8'4 +766 h '4 #tDisinfecting external Intake with kill C'!& h &W C'g% h %4 .t\CSV.dial CSR C';  +38 h  C'r% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'~' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'+ '  +38 h +75 .t\CTV.dial :intake C'.  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C')' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'w  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Z'4 +607 h '4 .t\CSV.dial CSR C'j  +113 h  C'3% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'o'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'c' +1372 h ' .t\CSV.dial CBV C'_ +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&G& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&*/ +150 h / .t\CC.seek :open C'R( +415 h ( M&p& h &ӷ M&d% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &z(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &q'η +91 h 'η .tHand.open M&$1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C',& h & .tHand.open M&v1 +2 h 1 M&A& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&>'η +91 h 'η .tHand.close M&D/ +150 h / M&& h &- M&/% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&:& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'((W +416 h (W C'& h &W .tHand.open M&K1 +150 h 1 M&I% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'?  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'L'[ +463 h '[ .t\CSV.dial :waste C'Y +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'V'4 +2010 h '4 #t\Purging external Intake C' & h &W C'l% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'f'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'O  +76 h #t\Flushing upper manifold C'&& h &W C'}% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C' % h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'|'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +506 h '0 .t\CSV.dial :waste C'i +38 h .t\CS.seek 0ml C''4 +507 h '4 C'e& h &W C'u% h %4 .t\CSV.dial :flush C'k  +37 h  .t\CS.seek 3ml C'^'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'# @ +37 h @ C'7% h %0 .t\CS.seek 5ml C'M'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'H'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +506 h '0 .t\CSV.dial :waste C'W +76 h .t\CS.seek 0ml C'%'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&U& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.seek CC M&]' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'y( +416 h ( M&& h &ӷ M& % h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&{1 +150 h 1 M&5% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Extending 18tundeployv2 until completion of MFBPCR +263687 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2517h1500ml.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*[  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +87 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *!'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*r'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +21 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *3'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +31 h  ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*t% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *a' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*J  +22 h F*p% h % ."MFBPCR"\AS.seek 0.81ml F*d'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*b'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*=  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp18oct2519h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*:  +10 h  F*@% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*I  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*L  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*l'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*V% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*O'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*&  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*f  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp18oct2519h1500ml.pcr F*|% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*#% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*H'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*x% h %n ."MFBPCR"\AS.seek 0.82ml F*U'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*`  +64 h  F*0% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *5' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +21 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*"'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*['m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*2% h %m ."MFBPCR"\AS.seek 0.82ml F*P'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*g  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb18oct2521h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ARV.dial :carrier F*3  +11 h  F*Z% h %V ."MFBPCR"\AS.seek 0.5ml F*o'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *G'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F**  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +21 h ."MFBPCR"delay 2 seconds +200 F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*p n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*6' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*$ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*v'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*A n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*9'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*z  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*>% h % \\ ."MFBPCR"\AS.seek 0.326ml F*:'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*@  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*S % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb18oct2521h1500ml.pcr F*h% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*K% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*L'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Y  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *V'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*O  +32 h  ."MFBPCR"\ATV.dial :air3 F*f % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*u'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*#' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V @CEST1540504821.30 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2600h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*0  +43 h  ."MFBPCR"\ARV.dial :carrier F*U  +10 h  F*<% h %V ."MFBPCR"\AS.seek 0.5ml F*m'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *F'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*N I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*C% h %Fj ."MFBPCR"\AS.seek 0.524ml F*5' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*4 n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Q  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*g  +107 h  ."MFBPCR"delay 7 seconds +700 F*P% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.6ml F*''\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*+% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*)% h % \\ ."MFBPCR"\AS.seek 0.326ml F*~'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*Z % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2600h1500ml.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*c  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +87 h % F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +21 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*d% h %n ."MFBPCR"\AS.seek 0.82ml F*6'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*b  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*9 % +87 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*@'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*R  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2602h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*L  +11 h  F*V% h %V ."MFBPCR"\AS.seek 0.5ml F*l'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *}'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*T % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*X  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*e% h % ."MFBPCR"\AS.seek 0.536ml F*\ '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +22 h  ."MFBPCR"delay 7 seconds +700 F*u% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*# n +21 h n ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*^'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*7% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*x  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*H  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*E% h % \\ ."MFBPCR"\AS.seek 0.326ml F*0'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2602h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*` @@T ."MFBPCR"\ASV.dial :SPEdelivery F*m  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*(  +151 h F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *s' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*{  +43 h  F*]% h % ."MFBPCR"\AS.seek 0.62ml F*N'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*C n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *F'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *4'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*5 % +43 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *2' +424 h ' ."MFBPCR"\ATV.dial :air3 F*"% h % ."MFBPCR"\AS.seek 0.53ml F*g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*[% h % ."MFBPCR"\AS.seek 0.71ml F*!' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h % H ."MFBPCR"\AS.seek 0.735ml F*r' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*'% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*)% h % zG ."MFBPCR"\AS.seek 0.365ml F*.' +7161 h ' ."MFBPCR"\ATV.dial :waste F*3 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*j % +43 h % F*~% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*G% h % ."MFBPCR"\AS.seek 0.579ml F*o' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %  ."MFBPCR"\AS.seek 0.604ml F**'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F*a% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*B' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*d  +22 h  ."MFBPCR"\AS.seek 0ml F*J'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *b'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*6% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*=% h % ."MFBPCR"\AS.seek 0.58ml F*A' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h % q ."MFBPCR"\AS.seek 0.605ml F*D'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*/% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*q' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*> n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*z'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *h'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *:'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*R n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*K'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *L': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*V  +65 h  F*Y% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *l'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*O I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*}  +65 h  F*T% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *|'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*X% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *e' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*u  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *\ ': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*# n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *k'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*M'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*7  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*^ % +43 h % F*x% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*W% h % ."MFBPCR"\AS.seek 0.96ml F*U'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %0 ."MFBPCR"\AS.seek 0.985ml F*0' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F* % h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *]'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *C'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *F'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*4  +43 h  F*5% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Q' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9987 h ' ."MFBPCR"\ATV.dial AIV F*" I +43 h I ."MFBPCR"\AS.seek 0.02ml F*2' +9880 h ' ."MFBPCR"\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.seek 0ml F*['V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*g% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +43 h % F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *''Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*)'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*.'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +21 h F*~% h %n ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*_  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*G  +65 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*d  +31 h  ."MFBPCR"\ATV.dial :air3 F*B % +87 h % F*J% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*6% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*A'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *:'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*I % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tundeployv2 assays .GoodNews.suppressMail "{1 => 22, 2 => 22, 3 => 22, 4 => 9, 5 => 13, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:49:35CEST30-Sep-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tundeployv2 assays" #\Mission runtime was 1:09:43.865937 #-> exit #m\EXIT @1538301077.25CEST @1539673834.79CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 05:41:37CEST26-Oct-18 .delayUntil "05:41:37.00CEST26-Oct-18" @CEST1540525297.00 #-> exit #m\EXIT @1539673958.96CEST @1539675068.48CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 05:41:37CEST26-Oct-18 .delayUntil "05:41:37.00CEST26-Oct-18" @CEST1540525297.00 #-> execute 18tundeployv4.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"{compile error\ (ESP):1: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeployv4.rb\ ^ #-> execute 18tundeployv4 !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):2: syntax error, unexpected tIDENTIFIER, expecting $end #-> execute 18tundeployv4 !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):3: syntax error, unexpected tIDENTIFIER, expecting $end #-> execute '18tundeployv4' !o:RuntimeError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+Mission's startTube should be 5, not 3 #-> exit #m\EXIT @1539675335.32CEST @1539675370.27CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tundeployv4' .GoodNews.suppressMail "Starting 18tundeployv4 w/pucks in tube 3\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:36:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployv4 begun" #\Preparing to sleep until 14:00 16-oct-18 #"shutdown"\Shutdown #t\Asleep til 14:00 16-oct-18 #\Continuing at 14:00CEST16-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeployv4.rb:118\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:37:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 14:00CEST16-Oct-18" @CEST1539691200.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:37:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'R 'Q  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 09:37:16CEST16-Oct-18\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:37:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +65 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M& & h &- M&\ % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +166 h ( M& & h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h & .t\SE.seek 20.71 Pucks below Top Plate A(' +56 h ' A(& h & M&& h & .t\Elbow.seek 3 M&( +7 h ( M&& h & A(& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&& h & M&% h %B .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)/% C'''4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +1090 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C'99 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'Q9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'R9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A(9'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +18 h @ .t\CS.seek 10ml C'J' +6 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Q& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&R(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&Z% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +265 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +37 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV P%j  .t\CBV.dial PRV C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +38 h  .t\PS.seek 2.5ml P%o' +2609 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2610 h ' .t\PSV.dial :air P%u  +37 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +38 h .t\PS.seek 0ml P%n' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C' & h & M&z% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (w& h & M& & h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A( & h & .t\SE.seek 19.71 Pucks below Top Plate A(' +65 h ' A(& h & M&#& h & .t\Elbow.seek 3 M&( +7 h ( M&& h & A((& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(s'X h & M&,% h %B .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +143 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +90 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C')'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :mixloop P%: +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%'  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +150 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +18 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&N% h %\  A(O% h %X A(Q. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (M( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (S& h &  M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +142 h ( M&P& h & A(9& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &1' +90 h ' A(& h &  .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +264 h '\\ M&W& h & A(T& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &Z' +25 h '  .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(V- h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +27 h ' M&\\% h %  A(R& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&U'8 +21 h '8 M&&& h & A(`& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  .t\Elbow.seek CC M&e(ӷ +142 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h &  A(p& h &  M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &C( +143 h ( M&o& h & A(u& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &t' +92 h '  .t\Elbow.hold M&?, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %  A(q& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&{'8 +21 h '8 M&~& h & A([& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P%\ ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'z  +38 h .t\PRV.dial between CBV and air P%  +18 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +251 h ' .t\PRV.dial :waste2 P%} @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M&$/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&,'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +37 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%'  +75 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +75 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%)' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%Q  +75 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %9'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1312 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :PML P%T +37 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'R& F*& @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1598 h ' .t\PSV.dial PSR P%U +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%Y' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P%z +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +37 h  .t\PS.seek 0ml P%\ ' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%} +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%$  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%(  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*/  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*s' +170 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*l'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F**% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV C''  +38 h  +113  .t\CBV.dial :waste1 C'! @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +22 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +37 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'6'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*) n +107 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'Q& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'9( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'L& h & M&@% h %\  A(g% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (1( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h & M&^& h &- M&_% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Y( +166 h ( M&j& h & A(4& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(p& h & ."WCR"\SE.seek 18.71 Pucks below Top Plate A(Z' +77 h ' A(K& h & M&;& h & ."WCR"\Elbow.seek 3 M&d( +7 h ( M&u& h & A(e& h & ."WCR"\Forearm.seek 3 + 300 counts M&x'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &k/ +150 h / ."downElevator"\SE.seek :down A(o'X M&q& h & M&[% h %B ."WCR"\Forearm.seek :retract M&]'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' ."WCR"\CC.seek :closed C'v(W +3 ."MFBPCR"\ATV.dial :waste2 F*t % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&b1 +150 h 1 M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +7 h + S)8% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *E' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*)% h % ."MFBPCR"\AS.seek 0.279ml F*D'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*H n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*G % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *Q'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*S I +22 h I F*=% h %v ."MFBPCR"\AS.seek 0.059ml F*P'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*"% h % ."MFBPCR"\AS.seek 0.769ml F*%' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +21 h % ."MFBPCR"delay 3 seconds +300 F*\\% h % q ."MFBPCR"\AS.seek 0.794ml F*R' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*a% h % k ."MFBPCR"\AS.seek 0.779ml F*f'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*T I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *L' +4416 h ' ."MFBPCR"\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.seek :empty F*g'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*U% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*`% h % ."MFBPCR"\AS.seek 0.579ml F*j' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*;% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +43 h  ."MFBPCR"\AS.seek 0ml F*k'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *['V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*Y % +108 h % F*X% h %V ."MFBPCR"\AS.seek 0.59ml F*i' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*C n +108 h n ."MFBPCR"\ASV.dial :wasteR F*x% h %  ."MFBPCR"\AS.seek 0.09ml F*Z' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*o I +85 h I F*d% h % ."MFBPCR"\AS.seek 0.12ml F*]' +626 h ' ."MFBPCR"\ATV.dial :air3 F*9 % +21 h % F*I% h % ."MFBPCR"\AS.seek 0.13ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*c% h % ."MFBPCR"\AS.seek 0.23ml F*8' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*~'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*1% h %X ."MFBPCR"\AS.seek 0.24ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*z n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*M I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*$'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*(% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*/% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *#' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +3472 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1615h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*s  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *l'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*+ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*.' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*!  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*''< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*F% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*E  +43 h  ."MFBPCR"\ASV.dial :waste1 F*H  +65 h ."MFBPCR"\ARV.dial :air1 F*D  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*S % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226124 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'=  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'Q @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)P% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'"% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'B' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\\  '%  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'N'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'a  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'R' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'T  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +38 h  C'@% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'U' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'L @ +75 h @ +50 S)g% h % ."WCR"\CBV.dial between CSV and waste2b C'_  +94 h  C'`% h % ."WCR"\CSV.dial between air and CTV C'^ ` +57 h ` ."WCR"\CS.seek 7.288ml C'4' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'p  'j  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C';  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'e  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'V  +38 h  C'u% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'k' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'Y @ +75 h @ +50 S)X% h % ."WCR"\CBV.dial between CSV and waste2b C'[  +95 h  C'C% h % ."WCR"\CSV.dial between air and CTV C'x ` +56 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C't  ."WCR"\CSV.dial CTV C'o  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'Z  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '9' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +151 h C']& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&{% h %\  A(c% h %X A(b. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&8& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &1( +142 h ( M&~& h & A(z& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(r& h &  ."WCR"\SE.seek 0.71 Pucks below Top Plate A(w'K +253 h 'K M& & h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &M' +26 h '  ."WCR"\Elbow.hold M&\ , +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M& % h %  A(}& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A((' +37 h ' M&& h & M&/% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M&#& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C',( +416 h ( A(?& h &  A(& h &  M&& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&5'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &s( +143 h ( M&& h & A(*& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +92 h '  ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &01 +150 h 1 M&l% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &7'8 +21 h '8 M&& h & A(!& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A(.% h % ."downElevator"\SE.seek :down A(A'X +100 (F% h %B M&'% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +90 h '\  +51 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&21 +2 A(6 h 1 M&>& h & M&<% h %\  .t\Elbow.seek FlushPuck::Garage M&J(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.seek :garage M&D'η +91 h 'η .tHand.close M&G/ +150 h / M&)& h &- M&6% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'& h &W .tHand.open M&"1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'%& h &W C'\\% h % .t\CSV.dial CSR C'B  +38 h  C'a% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'N'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'T% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +462 h '[ .t\CSV.dial :waste C'@ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'L% h %4 .t\CSV.dial :flush C'g  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '_'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'^% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV C'j  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +75 h @ .t\CS.seek 0ml C';'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'e% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +37 h @ C'k% h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'X  +38 h  .t\CBV.dial :waste2b C'[  +75 h  .t\CS.seek 0ml C'C'4 +766 h '4 #t\Flushing upper manifold C'x& h &W C'q% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +37 h @ C'Z% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I ']  +38 h +75 .t\CTV.dial :air C'{  +151 h .t\CS.seek 0ml C'c'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'8'4 +507 h '4 C'& h &W C'~% h %4 .t\CSV.dial :flush C'z  +37 h  .t\CS.seek 3ml C'r'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'y% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'}'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C' % h %4 .t\CSV.dial CSR C'  +151 h  C'/% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'1' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w +113 h .t\CTV.dial :intake C'M  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'#' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'm  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'$% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'\ 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'v  +38 h  +113  .t\CBV.dial :waste1 C'? @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'0'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'l  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'. '5  +37 h +76 .t\CTV.dial :air C's  +150 h .t\CS.seek 0ml C'F'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'''0 +507 h '0 .t\CSV.dial :waste C': +37 h .t\CS.seek 0ml C'2'4 +507 h '4 #t\Flushing upper manifold C'>& h &W C'<% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'7'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +37 h @ C'n% h %p .t\CS.seek 7ml C'E'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C')& h &W C'6% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C'D'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +38 h @ C'O% h %0 .t\CS.seek 5ml C'J'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'S'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'%% h %4 .t\CSV.dial :flush C'\\  +37 h  .t\CS.seek 5ml C'B'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CTV C'T  .t\CTV.dial CSV C'N  +38 h  +113  .t\CBV.dial :waste1 C'@ @ +37 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'L% h %4 .t\CSV.dial :flush C'g  +76 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'^% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'p  +38 h  .t\CBV.dial :waste2b C'j  +75 h  .t\CS.seek 0ml C'4'4 +766 h '4 #tDisinfecting external Intake with kill C'U& h &W C'K% h %4 .t\CSV.dial CSR C'e  +38 h  C'V% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C';'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'W' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'k 'u  +37 h +75 .t\CTV.dial :intake C'X  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'[' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Y  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'x'4 +606 h '4 .t\CSV.dial CSR C'q  +113 h  C'o% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'C'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'i' +1372 h ' .t\CSV.dial CBV C'Z +113 h .t\CS.seek 0ml C't'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&]& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +7 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&c' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'b( +416 h ( M&& h &ӷ M&~% h % .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &8(- +264 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &r'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&w/ +150 h / M&M& h &- M&1% h %η .t\Forearm.seek :retract M&m'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'\ (W +416 h (W C'& h &W .tHand.open M&v1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C'l +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'0& h &W C'% h %4 .t\CSV.dial :flush C'5  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C's +75 h .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'F'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C':  +75 h #t\Flushing upper manifold C''& h &W C'>% h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +37 h @ C'(% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 +113 h .t\CTV.dial :air .\CS.seek 0ml C'H'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C')% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.seek 3ml C'3'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'Q% h %0 .t\CS.seek 5ml C'O'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'D'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'S +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&a/ +150 h / .t\CC.seek :open C'( +416 h ( M&B& h &ӷ M&T% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&g1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  #t\Completed [coreNEG] begun 14:00:16 #\Preparing to sleep until 14:00 17-oct-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00 17-oct-18 #\Continuing at 14:00CEST17-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:122\\n{1 => 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:38:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST17-Oct-18" +279952 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1615h.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*p  +22 h ."MFBPCR"\ATV.dial :air3 F*j % +86 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*V'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*u'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +64 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig F*#,,'T(^20( b ."MFBPCR"\AS.seek 0.47ml F*o'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*d  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1617h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*$% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*?  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*l' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F** % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*'  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*F  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*(% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*n % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1617h.pcr F*H% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*Q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +21 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*D'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +21 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +21 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1620h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*W  +42 h  ."MFBPCR"\ARV.dial :carrier F*o  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*h % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.53ml F*I' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1620h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*b  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*v  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F** % +43 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *l' +424 h ' ."MFBPCR"\ATV.dial :air3 F*5% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*!% h % ."MFBPCR"\AS.seek 0.71ml F*.' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h % H ."MFBPCR"\AS.seek 0.735ml F*>' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*F% h % Ű ."MFBPCR"\AS.seek 0.72ml F*2' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*( n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*<% h % zG ."MFBPCR"\AS.seek 0.365ml F*7' +7162 h ' ."MFBPCR"\ATV.dial :waste F*n n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *E' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*)% h % ."MFBPCR"\AS.seek 0.579ml F*A' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*O% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*Q' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +85 h n ."MFBPCR"\ASV.dial :wasteR F*J  +22 h  ."MFBPCR"\AS.seek 0ml F*D'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *\\'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*a % +107 h % F*,% h %V ."MFBPCR"\AS.seek 0.01ml F*#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*\ % h % ."MFBPCR"\AS.seek 0.58ml F*B' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h % q ."MFBPCR"\AS.seek 0.605ml F* '\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*g% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*N n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*L  +22 h  ."MFBPCR"\AS.seek 0ml F*f'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*&  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *j' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *p'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *R'R +202 h 'R ."MFBPCR"\SPE.coast F*e  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*4 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*;  +43 h  F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *k' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*X I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*[  +43 h  F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *o': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*x  +65 h  F*q% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *C'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*i n +64 h n F*Z% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *]' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*d  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*P  +65 h  F*h% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *~'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*c n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *y' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*I I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *z': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b */'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*r'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*w  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*} % +43 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h % ."MFBPCR"\AS.seek 0.96ml F*$'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h % ."MFBPCR"\AS.seek 0.97ml F*m'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F**% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *t' +9988 h ' ."MFBPCR"\ATV.dial AIV F*5 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*l' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*!'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*<'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +21 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*7'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*E% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*J  +31 h  ."MFBPCR"\ATV.dial :air3 F*Q % +87 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *p'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*R % +107 h % ."MFBPCR"\AS.seek 0ml F*4'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539777600.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ); h  C' 'V h  P% %U h  F* *X h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ C' 2\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *k  h  .t\SPE.configure SPEconfig F*\ 2 *u% h %V .t\AS.configure ASconfig S)[% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'W& h & S)#.0X 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:39:34CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'?& h & .tHand.open M&1 +150 h 1 M&& h &M M&m% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &v(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&*% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +264 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'l& h &W .tHand.open M&!1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C's% h %4 .t\CSV.dial CSR C'>  +150 h  C'(% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'F'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +2009 h '4 #t\Purging external Intake C'& h &W C'7% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'E '  h +75 .t\CTV.dial :intake C'+  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'G'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'A  +75 h #t\Flushing upper manifold C'3& h &W C'O% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'J'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Q @ +37 h @ C'D% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CBV C'a .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C','4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'%'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'\ '4 +507 h '4 C'\\& h &W C'T% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 3ml C'#'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'B% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'N'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C'9'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'e( +416 h ( M&K& h &ӷ M&R% h % .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&-1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 09:39:40CEST16-Oct-18\\n{1 => 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:39:34CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*4% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&;% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(V% h %X A(U. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +6 F*k% h %&K ."readyMFB"\ATV.dial :waste2 F*u % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *[' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (W& h & M&Y& h &- M&x% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &q( +166 h ( M&o& h & A(i& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(C& h & .t\SE.seek 17.71 Pucks below Top Plate A('* +86 h '* A(]& h & M&=& h & .t\Elbow.seek 3 M&d( +7 h ( M&& h & A(h& h & .t\Forearm.seek 3 + 300 counts M&{'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down A( 'X M&P& h & M&~% h %B .t\Forearm.seek :retract M&y'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&b1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  .t\SC.coast #collectSample 300ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)}+ +7 h + S)r% h % C'?& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Hd +100 C'l9 h 9Ld +100 C'!9 h 9Pd +100 C'9 h 9Td +100 C'9 h 9Xd +100 C'29 h 9(\\d +100 C'F9 h 9@`d +100 C'<9 h 9Wdd +100 C'n9 h 9nhd +100 C'9 h 9ld +100 C'79 h 9pd +100 C'9 h 9td +100 C'(9 h 9xd +100 C' 9 h 9|d +100 C'+9 h 9d +100 C'A9 h 9d +100 C')9 h 9'd +100 C'99 h 9>d +100 C'O9 h 9Td +100 C'E9 h 9kd +100 C'69 h 9d +100 C'G9 h 9d +100 C'D9 h 9d +100 C'J9 h 9d +100 C'a9 h 9d +100 C'S9 h 9d +100 C',9 h 9 d +100 C'H9 h 9 d +100 C'39 h 96d +100 C'%9 h 9Ld +100 C'\\9 h 9bd +100 C'T9 h 9xd +100 C'Q9 h 9d +100 C'\ 9 h 9d +100 C'9 h 9d +100 C'B9 h 9d +100 C'"9 h 9d +100 C'#9 h 9d +100 C' 9 h 9d +100 C'L9 h 9(d +100 C'f9 h 9>d +100 C'g9 h 9Td +100 C'9 h 9id +100 C'9 h 9d +100 C'&9 h 9d +100 C'N9 h 9d +100 C'j9 h 9d +100 C'.9 h 9d +100 C'9 h 9d +100 C'R9 h 9  d +100 C'@9 h 9 d +100 C'09 h 9 *d +100 C'_9 h 9 ?d +100 C'`9 h 9 Td +100 C'-9 h 9 j d +100 C'K9 h 9 $d +100 C'^9 h 9 (d +100 C'p9 h 9 ,d +100 C'49 h 9 0d +100 C';9 h 9 4d +100 C'V9 h 9 8d +100 C'U9 h 9 % h %X A(l. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (!( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &ӷ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &F( +142 h ( M&<& h & A(n& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(7& h &  .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': M&(& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &+' +25 h '  .t\Elbow.hold M&A, +7 h , .t\Elbow.reconfigure TightElbow A()- h -+: M&$&.,(\ #b .t\SE.seek 2.04 Pucks below Top Plate A(9' +27 h ' M&O% h %  A(E& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&6'8 +21 h '8 M&G& h & A(D& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(a' +36 h ' M&S& h & M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +90 h '\  .t\Elbow.seek CC M&3(ӷ +142 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&Q/ +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h &  A(B& h &  M&"& h &ӷ M&#% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +142 h ( M&f& h & A(g& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &&1 +150 h 1 M&N% h %  A(j& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&.'8 +21 h '8 M&& h & A(R& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &@' +22 h ' A(0% h % ."downElevator"\SE.seek :down A(_'X +100 (`% h %1 M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'^ @ +2 A(6 +17 h @ .t\CS.seek 10ml C'p' +7 h ' .t\CSV.dial between air and CTV C'4 ` +19 h ` .t\AIV.dial :bypass C'; { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'V& h & .tHand.open M&U1 +2 h 1 M&k& h & M&u% h %\  .t\Elbow.seek FlushPuck::Garage M&|(- +165 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&o& h &- M&i% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C']& h &W .tHand.open M&P1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%h +150 h P%~% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%q' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%Z  +37 h  .t\PS.seek 0ml P%/' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'{  P%  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%y  h .t\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial :air P%b  +38 h  .t\PS.seek 2.5ml P%e' +2609 h ' .t\PSV.dial :mixloop P%r +38 h .t\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%m +38 h .t\PS.seek 0ml P%}' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&X& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&[/ +150 h / .t\CC.seek :open C'I( +416 h ( M&*& h &ӷ M&M% h % .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C''& h & M&:% h %\  A(s% h %X A(>. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (l( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &<( +166 h ( M&n& h & A(7& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(z& h & .t\SE.seek 16.71 Pucks below Top Plate A(('; +95 h '; A( & h & M&)& h & .t\Elbow.seek 3 M&O( +7 h ( M&E& h & A(G& h & .t\Forearm.seek 3 + 300 counts M&D'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ +150 h / ."downElevator"\SE.seek :down A(,'X M&& h & M&F% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&+(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +90 h ' .t\CC.seek :closed C'9(W +416 h (W .tHand.open M&J1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'H  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'B' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'"'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%#  +38 h  .t\PS.seek 2.5ml P%f' +2610 h ' .t\PSV.dial :mixloop P%g +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%T  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%3 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%&  +150 h  .t\PS.seek :empty P%N' +2610 h ' .t\CBV.dial :waste1 C'j @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'R @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'0  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'L @ +38 h @ .t\CS.seek 10ml C'`' +2647 h ' .t\CSV.dial CTV C'-  +38 h  .t\CS.seek 0ml C'^'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'4  +150 h C';& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&V% h %\  A(U% h %X A(k. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (u( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (.& h &  M&a& h &ӷ M&@% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +142 h ( M&x& h & A(!& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &_' +90 h ' A(& h &  .t\SE.seek 2.71 Pucks below Top Plate A(') +234 h ') M&& h & A(o& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &i' +26 h '  .t\Elbow.hold M&K, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+) .t\SE.seek 3.04 Pucks below Top Plate A(' +27 h ' M&=% h %  A(|& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&C'8 +20 h '8 M&\ & h & A(]& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &P' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(W' +37 h ' M&h& h & M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&Z(ӷ +142 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&/' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'{( +416 h ( A( & h &  A(y& h &  M&& h &ӷ M&b% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &r( +143 h ( M&e& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &?' +92 h '  .t\Elbow.hold M&m, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&X% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&[& h & A(w& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(% h % ."downElevator"\SE.seek :down A(*'X +100 (M% h %  M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%v  + A(6 +112 h  P%8% h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%1  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%'  +37 h .t\CBV.dial AIV C':  +38 h .t\PRV.dial between CBV and air P%s  +19 h  .t\PRV.dial :air P%>  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%2 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %5' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&71 +2 h 1 M&z& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.seek :garage M&G'η +91 h 'η .tHand.close M&/ +150 h / M&S& h &- M&% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'((W +416 h (W C'D& h &W .tHand.open M&O1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'I& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%A  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%H  +75 h  .tdelay 2 seconds +200 P% % h % .t\PS.seek 1.499ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%l' +1094 h ' .t\PSV.dial :air P%p  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%B +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%#  +38 h  .tdelay 2 seconds +200 P%}% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %g'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%T  +75 h .tdelay 2 seconds +200 P%3% h %r .t\PS.seek 0.475ml P%&' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%f  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%j'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%R  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %0' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%L @ +76 h @ .t\PS.seek 0ml P%"' +2609 h ' .t\PSV.dial :air P%-  +76 h  .t\PS.seek 2.5ml P%`' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%4' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%;  +76 h  .t\PS.seek 2.5ml P%V' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%U +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%k  +75 h P%.% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %a' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'@ & +50 h & .t\AWV.select :waste C' +7 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %!'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %+' +1313 h ' .t\PSV.dial :air P%Q  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%o +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '=& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'|& F*\ @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air .\AIV.dial :bypass F*]  .t\PSV.dial PSR P%h +22 h  ."MFBPCR"delay 20 seconds +91 h P%~% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%_' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*Y I +128 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *i'6 +66 .t\PSV.dial :waste1 P%q  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%C +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*9% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*b % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *e'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%/' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*Z  +22 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *X' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%[' +217 ."MFBPCR"\ATV.dial :AIV F*w I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*d' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P% +113 h P%?% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%W  +37 h  .t\PS.seek 0ml P%v' +740 h ' ."MFBPCR"delay 5 seconds +500 F*89 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F**% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%1 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%'' +2610 h ' .t\PSV.dial PSR P%: +113 h P%s% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%2 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %z'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %)' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*E I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *m'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%S' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %t' +5107 h ' .t\PSV.dial :air P%G  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*%  +43 h  F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *F'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*{ I +43 h I F*D% h %Q ."MFBPCR"\AS.seek 0.01ml F*O' +171 h ' .t\PSV.dial :mixloop P%, +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*\\'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*A  +85 h  F*H% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*5  +43 h F*6% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *p'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'l  +38 h  C'B% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'#'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'}' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'T  +37 h  +113  .t\CBV.dial :waste1 C'3 @ +38 h @ .t\CS.seek 0ml C'g'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*f  +21 h ."MFBPCR"delay 3 seconds +300 F*&% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'R  +38 h  C'j% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*L  +21 h F*0% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*-'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*" n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*`'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C';' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'4  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'U'4 +2648 h '4 C'k& h &W #tGaraging FlushPuck in Collection Clamp M&.& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +6 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&a' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'!( +416 h ( M&Q& h &ӷ M&+% h % .t\Forearm.seek :retract M&o'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&=& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &]'η +91 h 'η .tHand.open M&h1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'Y& h & M&% h %\  A(q% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (_( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (C& h & M&& h &- M&P% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +165 h ( M&& h & A(9& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(y& h & ."WCR"\SE.seek 15.71 Pucks below Top Plate A(i'L +106 h 'L A(K& h & M&b& h & ."WCR"\Elbow.seek 3 M& ( +7 h ( M&& h & A(Z& h & ."WCR"\Forearm.seek 3 + 300 counts M& 'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +148 h '&0 ."MFBPCR"delay 3 seconds +2 h / ."downElevator"\SE.seek :down M&& A(e'X h & M&w% h %B ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&/(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&X& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &[' +64 ."MFBPCR"\ATV.dial :waste2 F* % +27 h ' ."WCR"\CC.seek :closed C'?(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +399 h (W ."WCR"Hand.open M&W1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +11 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b **' +59 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)d+ +7 h + S)N% h % C'v& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'1  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h ."WCR"\SS.seek 0ml S)2' +216 h ' ."WCR"\SSV.dial CBV S)  +150 h  ."WCR"\CTV.dial :intake C'u  +75 h  ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +207 C'7% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*u I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*2'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *E'\  +1031 h '\  F*n% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*S'\  +1031 h '\  F*G% h %\  ."MFBPCR"\AS.seek 0.5ml F*t'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*%% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *)' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +3472 h 'V ."MFBPCR"\SPE.coast F*{  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1715h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*D  +64 h  ."MFBPCR"\ARV.dial :carrier F*,  +11 h  F*F% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F** n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*# n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*^' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +108 h  ."MFBPCR"delay 7 seconds +700 F*R% h %< ."MFBPCR"\AS.seek 0.594ml F*j'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*p'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*-  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*4% h % \\ ."MFBPCR"\AS.seek 0.326ml F*;'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226154 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'@  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'k @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'< @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'(  +94 h  C'Q% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'a' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'=  '+  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'V'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'h  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '|' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'o  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'~  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'Y @ +75 h @ +50 S)q% h % ."WCR"\CBV.dial between CSV and waste2b C'P  +94 h  C']% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'9' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'y  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'b  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'Z  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C'r% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'X' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'e'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'i  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '!' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'`  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&% h %\  A(c% h %X A(W. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (v( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (1& h &  M&N& h &ӷ M&M% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &_( +143 h ( M&[& h & A(s& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &:' +89 h ' A(d& h &  ."WCR"\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' M&'& h & A(>& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &u' +25 h '  ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(7- h -+ ."WCR"\SE.seek 4.04 Pucks below Top Plate A(' +26 h ' M&z% h %  A(2& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&n'8 +21 h '8 M&E& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(G' +36 h ' M&S& h & M&%% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&)& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ ."WCR"\Forearm.seek CC M&D' +91 h ' ."WCR"Hand.close M&,/ +150 h / ."WCR"\CC.seek :open C'F( +416 h ( A(m& h &  A( & h &  M&*& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&A'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &O( +142 h ( M&5& h & A(6& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &H' +93 h '  ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &B1 +150 h 1 M&\\% h %  A(#& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&l& h & A(3& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +23 h ' A(^% h % ."downElevator"\SE.seek :down A(&'X +100 (R% h % M&T% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +89 h '\  +22 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&j1 + A(6 + h 1 M&0& h & M&L% h %\  .t\Elbow.seek FlushPuck::Garage M&p(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&4/ +150 h / M&"& h &- M& % h %η .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &@(ӷ +264 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'(& h &W .tHand.open M&Q1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'=& h &W C'+% h % .t\CSV.dial CSR C'a  +38 h  C'h% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'V'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'o% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '~'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '|'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'Y% h %4 .t\CSV.dial :flush C'q  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'P'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'y  +38 h  +113  .t\CBV.dial :waste1 C'9 @ +75 h @ .t\CS.seek 0ml C'b'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'Z% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'w  +75 h  .t\CS.seek 0ml C'r'4 +766 h '4 #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +37 h @ C'i% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CBV C'` .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'c'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C'1 +38 h .t\CS.seek 0ml C'N'4 +506 h '4 C'M& h &W C'[% h %4 .t\CSV.dial :flush C's  +38 h  .t\CS.seek 3ml C'd'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +37 h @ C'8% h %0 .t\CS.seek 5ml C'''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'>'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C'z +75 h .t\CS.seek 0ml C'2'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'E& h &W C'% h %4 .t\CSV.dial CSR C'S  +151 h  C'%% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C':'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'_' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'u  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C')' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'{  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +710 h ' ."connect"\CSV.dial CTV C't  .t\CTV.dial CSV C'?  +38 h  +113  .t\CBV.dial :waste1 C'm @ +37 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'*  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '5'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'B'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'\\  +37 h  C'#% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'l'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A '^  +37 h +76 .t\CTV.dial :air C'O  +150 h .t\CS.seek 0ml C'R'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'T'0 +506 h '0 .t\CSV.dial :waste C'g +38 h .t\CS.seek 0ml C'j'4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'L% h %4 .t\CSV.dial :flush C'H  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +37 h @ C'% h %p .t\CS.seek 7ml C'-'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} +113 h .t\CTV.dial :air .\CS.seek 0ml C'&'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'4'4 +507 h '4 C'"& h &W C' % h %4 .t\CSV.dial :flush C'f  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'U% h %0 .t\CS.seek 5ml C'p'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +506 h '0 .t\CSV.dial :waste C'( +76 h .t\CS.seek 0ml C'Q'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'=% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 5ml C'a'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'<% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'o  'V  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'~'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'Y% h %4 .t\CSV.dial :flush C'q  +75 h  .t\CS.seek 5ml C'|'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +37 h @ C'% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'y  +75 h  .t\CS.seek 0ml C'9'4 +766 h '4 #tDisinfecting external Intake with kill C'C& h &W C'K% h %4 .t\CSV.dial CSR C'Z  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'b'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'w' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'r'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'X' +1372 h ' .t\CSV.dial CBV C'i +113 h .t\CS.seek 0ml C' '4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&c' +91 h ' .tHand.close M&1/ +150 h / .t\CC.seek :open C'W( +416 h ( M&M& h &ӷ M&[% h % .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &N(- +264 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &d'η +91 h 'η .tHand.open M&'1 +150 h 1 M&>% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&E1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &S(- +6 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&/ +150 h / M&u& h &- M&_% h %η .t\Forearm.seek :retract M&{'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' .t\CC.seek :closed C'(W +416 h (W C't& h &W .tHand.open M&?1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'*& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'5% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '6'[ +463 h '[ .t\CSV.dial :waste C'\\ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +2009 h '4 #t\Purging external Intake C'B& h &W C'l% h %4 .t\CSV.dial :flush C'^  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'A' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +75 h .t\CTV.dial :intake C'3  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'R'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'g  +75 h #t\Flushing upper manifold C'T& h &W C'0% h %4 .t\CSV.dial :flush C'L  +75 h  .t\CS.seek 5ml C'H'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +37 h @ C'G% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +507 h '0 .t\CSV.dial :waste C'& +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'v& h &W C'"% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'f'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'k% h %0 .t\CS.seek 5ml C'U'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'p'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'('4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.seek CC M&Q' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'<( +416 h ( M&a& h &ӷ M&o% h % .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Y'η +91 h 'η .tHand.open M&q1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until Fri Oct 19 14:00:15 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00CEST19-Oct-18 #\Continuing at 14:00CEST19-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:127\\n{1 => 0, 2 => 6, 3 => 16, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:41:21CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST19-Oct-18" +279981 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1715h300ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*y % +87 h % F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *C'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*Z% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +31 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*['> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % ."MFBPCR"delay 3 seconds +300 F*/% h %> ."MFBPCR"\AS.seek 0.835ml F*8'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +21 h F*'% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*s  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1718h300ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*W n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F**  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*5  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F* ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*6 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*l'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +107 h  ."MFBPCR"delay 7 seconds +700 F*#% h %< ."MFBPCR"\AS.seek 0.594ml F*O'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.65ml F*L' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*j  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1718h300ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*-  +22 h ."MFBPCR"\ATV.dial :air3 F*v % +86 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*q% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +21 h F*Y% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*P% h %m ."MFBPCR"\AS.seek 0.82ml F*|'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1720h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*M I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*/ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.53ml F*`' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*'  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*s'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +22 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*E'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*_  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*N  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1720h300ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*S @@T *W  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*t  +151 h F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b **' +423 h ' ."MFBPCR"\ATV.dial :ASV F*J n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*?  +43 h  F*5% h % ."MFBPCR"\AS.seek 0.62ml F*n'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *D'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\\' +423 h ' ."MFBPCR"\ATV.dial :air3 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*3% h % ."MFBPCR"\AS.seek 0.71ml F*A' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h % H ."MFBPCR"\AS.seek 0.735ml F*0' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*R% h % Ű ."MFBPCR"\AS.seek 0.72ml F*j' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*G n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*L% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*} % +43 h % F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *-' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*"% h % ."MFBPCR"\AS.seek 0.579ml F*&' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*U% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :wasteR F*p  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*h % +108 h % F*F% h %V ."MFBPCR"\AS.seek 0.01ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*a' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*q% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*!' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*V n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Y  +21 h  ."MFBPCR"\AS.seek 0ml F*~'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *y' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*P n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +202 h 'R ."MFBPCR"\SPE.coast F*Z  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*9 n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*b  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*w  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *r'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*X n +65 h n F*i% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*1 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *@'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*e  +43 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*x  +64 h  F*/% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *['Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*c n +65 h n F*'% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *8' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*` I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*7  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *s': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *%'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*E n +151 h n ."MFBPCR"\AS.seek 0ml F*d'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*2 % +43 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*u% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*m% h % ."MFBPCR"\AS.seek 0.97ml F*{'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*J I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *5'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*D  +43 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b * ' +9988 h ' ."MFBPCR"\ATV.dial AIV F*^ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*\\' +9880 h ' ."MFBPCR"\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.seek 0ml F*3'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*#% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*A % +43 h % F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +64 h  F*-% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*4' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*p  +32 h  ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +21 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *y'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*| % +108 h % ."MFBPCR"\AS.seek 0ml F*9'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539950415.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )b h  C' ' h  P% %C h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)w% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & S)#.0X'4 ."rdySampler"\SS.home.seek 0ml S)#.0X 0, 2 => 6, 3 => 16, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:42:02CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'm& h & .tHand.open M&1 +150 h 1 M&J& h &M M&{% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &?(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&n/ +150 h / M&D& h &- M&6% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +265 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +90 h ' .t\CC.seek :closed C'#(W +416 h (W C'\\& h &W .tHand.open M&31 +150 h 1 M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'O% h %4 .t\CSV.dial CSR C'0  +151 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'R'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'j% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'L +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +2010 h '4 #t\Purging external Intake C'H& h &W C'% h %4 .t\CSV.dial :flush C'v  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'- '  h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'4'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'&  +75 h #t\Flushing upper manifold C'f& h &W C'U% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h +113 h .t\CTV.dial :air .\CS.seek 0ml C'F'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +506 h '0 .t\CSV.dial :waste C', +38 h .t\CS.seek 0ml C''4 +506 h '4 C'+& h &W C'o% h %4 .t\CSV.dial :flush C'Q  +38 h  .t\CS.seek 3ml C')'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'a% h %0 .t\CS.seek 5ml C'q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'Y +75 h .t\CS.seek 0ml C'!'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&~& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&P' +91 h ' .tHand.close M&y/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&K& h &ӷ M&|% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&91 +150 h 1 M&$% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +90 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 09:42:07CEST16-Oct-18\\n{1 => 0, 2 => 6, 3 => 16, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:42:02CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*(% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'S& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&b% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(C. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +7 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *w' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&& h & A(X& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(r& h & .t\SE.seek 14.71 Pucks below Top Plate A(1'] +116 h '] A(& h & M&@& h & .t\Elbow.seek 3 M&e( +7 h ( M&M& h & A(& h & .t\Forearm.seek 3 + 300 counts M&/'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down M&'& h & A(x'X M&[% h %B .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.seek CC M&E(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &N' +91 h ' .t\CC.seek :closed C'z(W +416 h (W .tHand.open M&W1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  .t\SC.coast #collectSample 300ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)2+ +7 h + S)d% h % C'm& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*j  h  ."readyMFB"\ASV.dial :waste1 F*R  h +1311 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'L% h %4 S)% h % S)H% h % .tIntake.select :close S)0 +6 )% h % .t\SSV.dial :exhaust S)v  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'U9 h 9Td +100 C' 9 h 9kd +100 C' 9 h 9d +100 C'49 h 9d +100 C'9 h 9d +100 C'p9 h 9d +100 C'h9 h 9d +100 C'.9 h 9d +100 C'F9 h 9 d +100 C'}9 h 9 d +100 C'f9 h 96d +100 C'=9 h 9Ld +100 C'+9 h 9bd +100 C'o9 h 9xd +100 C'k9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'a9 h 9d +100 C'Q9 h 9d +100 C')9 h 9d +100 C'9 h 9d +100 C'Y9 h 9(d +100 C'~9 h 9>d +100 C'q9 h 9Td +100 C';9 h 9id +100 C',9 h 9d +100 C'\ 9 h 9d +100 C'V9 h 9d +100 C'y9 h 9d +100 C'A9 h 9d +100 C'#9 h 9d +100 C'|9 h 9  d +100 C'I9 h 9 d +100 C'B9 h 9 *d +100 C'P9 h 9 ?d +100 C']9 h 9 Td +100 C'99 h 9 j d +100 C'K9 h 9 $d +100 C'9 h 9 (d +100 C'9 h 9 ,d +100 C'(9 h 9 0d +100 C'b9 h 9 4d +100 C'9 h 9 8d +100 C'C9 h 9 . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (P( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (r& h & M&:& h &- M&'% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +165 h ( M&/& h & A(!& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &M' +90 h ' A(8& h & ."WCR"\SE.seek 12.71 Pucks below Top Plate A(X' +136 h ' A(K& h & M&W& h & ."WCR"\Elbow.seek 3 M&e( +7 h ( M&& h & A(i& h & ."WCR"\Forearm.seek 3 + 300 counts M&'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &7/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down M&5& A(Z'X h & M&% h %B ."WCR"\Forearm.seek :retract M&<'\ +91 h '\  ."WCR"\Elbow.seek CC M&%(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +35 ."MFBPCR"\ATV.dial :waste2 F*w % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +13 h ' ."WCR"\CC.seek :closed C'u(W +416 h (W ."WCR"Hand.open M&1 +131 h 'R ."MFBPCR"\ATV.dial :air2 F**  +19 h 1 M&N% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *6' +89 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)x+ +7 h + S)V% h % C'?& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'n  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*/' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*W% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*i n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*7'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*M% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*d n +107 h n ."MFBPCR"\ASV.dial :wasteR F*X% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*e I +86 h I F*Z% h % ."MFBPCR"\AS.seek 0.12ml F*<' +625 h ' ."MFBPCR"\ATV.dial :air3 F*] % +21 h % F*3% h % ."MFBPCR"\AS.seek 0.13ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +21 h F**% h % ."MFBPCR"\AS.seek 0.23ml F*V' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*P% h %X ."MFBPCR"\AS.seek 0.24ml F*x' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*O n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *l% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *0'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*J @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*; I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*j'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*D  +43 h  #"MFBPCR"\Swish #1 of 2 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b * '\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*_% h %(A ."MFBPCR"\AS.seek 0.6ml F*.'\  +1031 h '\  F*4% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*=% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *"' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*H % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +3472 h 'V ."MFBPCR"\SPE.coast F*1  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1915h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*G  +64 h  ."MFBPCR"\ARV.dial :carrier F*F  +11 h  F*R% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*^  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*z I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*) n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*f  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*o'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +108 h  ."MFBPCR"delay 7 seconds +700 F*|% h %< ."MFBPCR"\AS.seek 0.594ml F*y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +21 h n ."MFBPCR"delay 2 seconds +200 F*q% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*B  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 F*9  +64 h ."MFBPCR"\ARV.dial :air1 F*(  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*A % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226183 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'I  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'L @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'S% h % ."WCR"\CSV.dial between air and CTV C'v ` +19 h ` ."WCR"\CS.seek 7.288ml C'h' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'@  '-  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'C'4 +1920 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '$' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'[  +38 h  C',% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'>' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C''  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C': ` +57 h ` ."WCR"\CS.seek 7.288ml C'!' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'8  '/  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'W  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'i  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'7' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)M% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'd% h % ."WCR"\CSV.dial between air and CTV C'X ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'e  'w  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'Z'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ']' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'3  +150 h C'<& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&N% h %\  A(*% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (?( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (n& h &  M&V& h &ӷ M&m% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &P( +142 h ( M&& h & A(O& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &g' +90 h ' A(x& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(l' +195 h ' M&T& h & A(0& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &;' +26 h '  ."WCR"\Elbow.hold M&t, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b ."WCR"\SE.seek 7.04 Pucks below Top Plate A(D' +27 h ' M&s% h %  A(j& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M& & h & A(_& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A(4'w +37 h 'w M&.& h & M&=% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  ."WCR"\Elbow.seek CC M&H(ӷ +143 h (ӷ M&"& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ ."WCR"\Forearm.seek CC M&G' +90 h ' ."WCR"Hand.close M&F/ +150 h / ."WCR"\CC.seek :open C'R( +416 h ( A({& h &  A(& h &  M&6& h &ӷ M&p% h % ."WCR"\Forearm.seek :retract M&&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &U( +143 h ( M&^& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &}' +92 h '  ."WCR"\Elbow.hold M&z, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &a1 +150 h 1 M&+% h %  A()& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&\\& h & A(f& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &2' +22 h ' A(% h %w ."downElevator"\SE.seek :down A(\ 'X +100 (|% h % M&o% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&y1 +2 h 1 M&B& h & M&Y% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M&b/ +150 h / M&Q& h &- M&% h %η .t\Forearm.seek :retract M&A'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &I(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'& h &W .tHand.open M&S1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'@& h &W C'-% h % .t\CSV.dial CSR C'h  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'C'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'k% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '['[ +463 h '[ .t\CSV.dial :waste C', +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '$'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'>& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C':% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'8  '/  +37 h  +113  .t\CBV.dial :waste1 C'! @ +76 h @ .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'i% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'7% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'd'4 +766 h '4 #t\Flushing upper manifold C'X& h &W C'5% h %4 .t\CSV.dial :flush C'w  +75 h  .t\CS.seek 5ml C'e'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'Z'i +416 h 'i ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV C'<  +38 h +75 .t\CTV.dial :air C'N  +150 h .t\CS.seek 0ml C'*'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'n +38 h .t\CS.seek 0ml C'V'4 +506 h '4 C'm& h &W C'% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C'x'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +38 h @ C'T% h %0 .t\CS.seek 5ml C'0'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C's +75 h .t\CS.seek 0ml C'j'4 +507 h '4 #tDisinfecting external Intake with mfbkill C' & h &W C'_% h %4 .t\CSV.dial CSR C'.  +150 h  C'=% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'g'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'P' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l +113 h .t\CTV.dial :intake C';  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'"' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'1  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'D  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C't'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'u  '  +37 h  +113  .t\CBV.dial :waste1 C'{ @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'6  +37 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '^'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +37 h  .t\CBV.dial :waste2b C'H  +76 h  .t\CS.seek 0ml C'a'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'+  +38 h  C')% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'& '  +38 h +75 .t\CTV.dial :air C'U  +151 h .t\CS.seek 0ml C'|'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'o'0 +506 h '0 .t\CSV.dial :waste C'q +38 h .t\CS.seek 0ml C'y'4 +506 h '4 #t\Flushing upper manifold C'B& h &W C'Y% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'#% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'2 +113 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +506 h '0 .t\CSV.dial :waste C'z +38 h .t\CS.seek 0ml C'b'4 +507 h '4 C'Q& h &W C'% h %4 .t\CSV.dial :flush C'~  +37 h  .t\CS.seek 3ml C'('0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'A'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'R'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'S'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'v& h &W C'@% h %4 .t\CSV.dial :flush C'-  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'L% h %p .t\CS.seek 7ml C']'i +416 h 'i ."connect"\CSV.dial CTV C'k  .t\CTV.dial CSV C'C  +37 h  +113  .t\CBV.dial :waste1 C', @ +38 h @ .t\CS.seek 0ml C'['4 +766 h '4 #t\Flushing lower manifold's waste2b port C'>& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'$'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C':% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'8  +38 h  .t\CBV.dial :waste2b C'/  +75 h  .t\CS.seek 0ml C'!'4 +766 h '4 #tDisinfecting external Intake with kill C'r& h &W C'K% h %4 .t\CSV.dial CSR C'i  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'W'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C'7 .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'M  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +607 h '4 .t\CSV.dial CSR C'5  +113 h  C'w% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'e'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +6 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&*' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'( +416 h ( M&m& h &ӷ M&% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +265 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &x'η +91 h 'η .tHand.open M&01 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&_& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &.(- +6 h (- M&=& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.seek :garage M&g'η +91 h 'η .tHand.close M&l/ +150 h / M&;& h &- M&P% h %η .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +264 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C't(W +416 h (W C'u& h &W .tHand.open M& 1 +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'6& h &W C'p% h %4 .t\CSV.dial CSR C'  +113 h  C'^% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'G'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'H% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'+ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ')'4 +2010 h '4 #t\Purging external Intake C'a& h &W C'\\% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U +76 h .t\CTV.dial :intake C'f  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'|'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'q  +76 h #t\Flushing upper manifold C'o& h &W C'B% h %4 .t\CSV.dial :flush C'Y  +75 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +38 h @ C'4% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'2'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'\ +38 h .t\CS.seek 0ml C'z'4 +507 h '4 C'?& h &W C'Q% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'~'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'('4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'A +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&R& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&@& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'L( +415 h ( M&h& h &ӷ M&k% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &,(- +265 h (- M&]& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:30 #\Preparing to sleep until Sun Oct 21 14:00:30 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00CEST21-Oct-18 #\Continuing at 14:00CEST21-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:127\\n{1 => 0, 2 => 9, 3 => 13, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:43:49CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST21-Oct-18" +280002 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1915h300ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*8  +21 h ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *r'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +21 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Z  +31 h  ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*0% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1918h300ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*D  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*.'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +22 h ."MFBPCR"delay 2 seconds +200 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*^  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*e' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*H  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*+' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*\\'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*U'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*q'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*o  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*Y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*4% h % \\ ."MFBPCR"\AS.seek 0.326ml F*}'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1918h300ml.pcr F*2% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*9  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*-  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *"'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*>  +32 h  ."MFBPCR"\ATV.dial :air3 F*v % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*8'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*'% h %m ."MFBPCR"\AS.seek 0.82ml F*$'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1920h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*w  +11 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*d'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*n  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*I  +21 h ."MFBPCR"delay 2 seconds +200 F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*% % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*3' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*0  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +22 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*l% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*P  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*g% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*s % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1920h300ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*. @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*u  +151 h F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *6' +423 h ' ."MFBPCR"\ATV.dial :ASV F*p n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*^% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *G'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*e'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*f% h % ."MFBPCR"\AS.seek 0.71ml F*&' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h % H ."MFBPCR"\AS.seek 0.735ml F*B' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*|% h % Ű ."MFBPCR"\AS.seek 0.72ml F*y' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*4 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*# n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*2 % +43 h % F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*Q% h % ."MFBPCR"\AS.seek 0.579ml F*\ ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*A n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*('V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *-'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*R% h %V ."MFBPCR"\AS.seek 0.01ml F*"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*t% h % ."MFBPCR"\AS.seek 0.58ml F*h' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*S% h % q ."MFBPCR"\AS.seek 0.605ml F*>'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*]' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*C n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*['V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b */' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *8'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +202 h 'R ."MFBPCR"\SPE.coast F*i  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*r'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*! n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*W  +42 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *7' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*M I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *w': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*X  +64 h  F*5% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *d'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*n I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Z  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*%% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F** n +65 h n F*0% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *T' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*3 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *O': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *='Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*x'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*l  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*j % +43 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*;% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*D' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*{% h % ."MFBPCR"\AS.seek 0.97ml F*1'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*p I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *^'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *a' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *e' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*+' +9880 h ' ."MFBPCR"\ATV.dial :waste F*\\ n +129 h n ."MFBPCR"\AS.seek 0ml F*f'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*)% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*o  +21 h ."MFBPCR"\ATV.dial :air3 F*& % +43 h % F*q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *|'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*4% h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*J'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +64 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *z'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*(% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*R% h % ."MFBPCR"\AS.seek 0.81ml F*-'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*S% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *8'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*$ % +108 h % ."MFBPCR"\AS.seek 0ml F*!'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast .\Elbow.coast M&5 +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540123230.89 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )W h  C' ' h  P% %r h  F* *M h  C' 9\ ' 0\ P% 9\ % 2\ C' -\ P% *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *7  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 9, 3 => 13, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:44:30CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&D1 +150 h 1 M&p& h &M M&1% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +166 h (- M&^& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&G& h &- M& % h %η .t\Forearm.seek :retract M&H'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C')(W +416 h (W C'+& h &W .tHand.open M&f1 +150 h 1 M&o% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'U% h %4 .t\CSV.dial CSR C'B  +150 h  C'4% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '|'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '#'[ +463 h '[ .t\CSV.dial :waste C'Y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '2'4 +2009 h '4 #t\Purging external Intake C'}& h &W C'% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Q' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'9 'J  h +75 .t\CTV.dial :intake C'z  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'b'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'\  +75 h #t\Flushing upper manifold C'~& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'(% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'R'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +507 h '0 .t\CSV.dial :waste C'F +37 h .t\CS.seek 0ml C't'4 +507 h '4 C'-& h &W C'k% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 3ml C'"'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +38 h @ C'h% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'>'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C']'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&[& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'i( +416 h ( M&K& h &ӷ M&$% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &L(- +264 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +91 h 'η .tHand.open M&1 +150 h 1 M&`% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 09:44:35CEST16-Oct-18\\n{1 => 0, 2 => 9, 3 => 13, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@09:44:30CEST16-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*E% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'.& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&W% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(r. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (M( +6 F*7% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &5( +166 h ( M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(d& h & .t\SE.seek 11.71 Pucks below Top Plate A(n'Ɛ +145 h 'Ɛ A(<& h & M&I& h & .t\Elbow.seek 3 M&Z( +7 h ( M&m& h & A( & h & .t\Forearm.seek 3 + 300 counts M&%'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down M&N& A(0'X h & M&% h %B .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +55 h 'X ."downElevator"\SE.coast A(5 +87 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 300ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)j+ +7 h + S)x% h % C'{& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X exit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" exit #m\EXIT @1539675949.72CEST @1539675980.58CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> exit #m\EXIT @1539676298.26CEST @1539850510.70CEST = =ECan =tquick =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 01:00:00CET01-Jan-70 .delayUntil "01:00:00.00" #-> forgetESPstate !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::messageHundefined local variable or method `forgetESPstate' for main:Object: name:forgetESPstate #-> exit #m\EXIT @1539850569.07CEST @1539850611.36CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> ESP.ready? #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> execute 18tundeployv4.rb !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"{compile error\ (ESP):4: syntax error, unexpected tIDENTIFIER, expecting $end\ execute 18tundeployv4.rb\ ^ #-> execute '18tundeployv4.rb' .GoodNews.suppressMail "Starting 18tundeployv4 w/pucks in tube 3\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:17:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployv4 begun" #\Preparing to sleep until 14:00 18-oct-18 ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast #t\Asleep til 14:00 18-oct-18 S)5 ."shutdown"\CC.coast .\CS.coast C'6 #t\Continuing at 14:00CEST18-Oct-18 ."shutdown"\PC.coast E ;3\\0\\0 P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeployv4.rb:118\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:18:15, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 14:00CEST18-Oct-18" ."shutdown"\SC.coast A(6 ."shutdown"\SE.coast + M&6 A(5 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown @CEST1539864000.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) )M h  C' 'N h  P% %O h  F* *P h  C' 9\ ' 0\ P% 9\ % 2\ % *\ % 0\ C' -\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *Q  h  .t\SPE.configure SPEconfig F*\ 2 *R% h %R .t\AS.configure ASconfig S)S% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'T& S)#.0X 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:18:21, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&|1 +150 h 1 M&}& h &M M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C' (W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +151 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'"  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( '*  h +75 .t\CTV.dial :intake C'+  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C','4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'%% h %4 .t\CSV.dial :flush C''  +76 h  .t\CS.seek 5ml C'/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'1% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CBV C'3 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'4'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +506 h '0 .t\CSV.dial :waste C'6 +38 h .t\CS.seek 0ml C'7'4 +506 h '4 C'8& h &W C'9% h %4 .t\CSV.dial :flush C':  +38 h  .t\CS.seek 3ml C';'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +38 h @ C'=% h %0 .t\CS.seek 5ml C'>'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'?'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'@'0 +507 h '0 .t\CSV.dial :waste C'A +75 h .t\CS.seek 0ml C'B'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&C& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +6 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'I( +416 h ( M&H& h &ӷ M&K% h % .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &#(- +265 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &!'η +90 h 'η .tHand.open M&)1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:18:26CEST18-Oct-18\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:18:21, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*.% h %&K #tLoading sh1 puck from tube 3 into Collection Clamp #"readyMFB"Readying the MFB C'&& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&M% h %\  A(N% h %X A(O. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (P( +7 F*Q% h %&K ."readyMFB"\ATV.dial :waste2 F*R % +107 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *S' +87 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (T& h & M&U& h &- M&V% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &W( +166 h ( M&X& h & A(Y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &Z' +89 h ' A([& h & .t\SE.seek 20.71 Pucks below Top Plate A(\\' +57 h ' A(]& h & M&^& h & .t\Elbow.seek 3 M&_( +6 h ( M&`& h & A(b& h & .t\Forearm.seek 3 + 300 counts M&c'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &e/ +150 h / ."downElevator"\SE.seek :down M&f& A(g'X h & M&a% h %B .t\Forearm.seek :retract M&k'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&n(ӷ +143 h (ӷ M&l& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'w(W +416 h (W .tHand.open M&x1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'q& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S){% h % E ;3\\0\\0 C's'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '?'4 +2610 h '4 C'A% h %4 S)@% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'C @ +18 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'9 h 9Td +100 C'9 h 9kd +100 C'z9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'9 h 9 d +100 C'\ 9 h 96d +100 C' 9 h 9Ld +100 C'9 h 9bd +100 C'9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'*9 h 9d +100 C'9 h 9(d +100 C'+9 h 9>d +100 C'9 h 9Td +100 C'(9 h 9id +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'%9 h 9d +100 C',9 h 9d +100 C'19 h 9d +100 C'9 h 9  d +100 C'09 h 9 d +100 C'/9 h 9 *d +100 C'29 h 9 ?d +100 C''9 h 9 Td +100 C'39 h 9 j d +100 C'69 h 9 $d +100 C'59 h 9 (d +100 C'89 h 9 ,d +100 C'99 h 9 0d +100 C'79 h 9 4d +100 C'<9 h 9 8d +100 C'=9 h 9 9 h 9\ &Dd +100 C'49 h 9\ :Hd +100 C'A9 h 9\ OLd +100 C'@9 h 9\ cPd +100 C'C9 h 9\ xTd +100 C':9 h 9\ Xd +100 C'F9 h 9\ \\d +100 C'G9 h 9\ `d +100 C'9 h 9\ dd +100 C'E9 h 9\ hd +100 C'9 h 9\ ld +100 C'H9 h 9 pd +100 C'K9 h 9 td +100 C'J9 h 9 /xd +100 C' 9 h 9 C|d +100 C'D9 h 9 Wd +100 C'?9 h 9 kd +100 C'$9 h 9 d +100 C'9 h 9 d +100 C')9 h 9 d +100 C'L9 h 9 d +100 C'.9 h 9 d +100 C'-9 h 9 d +100 C'M9 h 9 d +100 C'&9 h 9 d +100 C'N9 h 9 d +100 C'O9 h 9 1d +100 C'Q9 h 9 Dd +100 C'T9 h 9 Xd +100 C'R9 h 9 kd +100 C'V9 h 9 ~d +100 C'U9 h 9 d +100 C'p9 h 9 d +100 C'X9 h 9 d .t\CH.hold 5:00,85C C'!   P h  +30000  C'[9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'Y'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'] @ +113 h @ .t\CSV.dial :air C'# @ +38 h @ .t\CS.seek 5ml C'b'p +1372 h 'p .t\CSV.dial CTV C'  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'e @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'f  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'a @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'o'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'W +113 h .t\CBV.dial CSV C'Z  +19 h C'l% h %4 .t\CS.seek 5ml C't'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'c  +37 h C'\\% h %p .t\CS.seek 7ml C'g'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'_ @ +38 h @ .t\CS.seek 10ml C'k' +859 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'I ` +132 h ` #tLysing Complete C'x& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&d% h %\  A(q% h %X A({. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (}( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (v& M&P& h &  ."raiseStack"\SE.seek :upperLimit h &ӷ A(B'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'r( +23 h 'Z +393 ( A(& h &  A(|& h &  M&u& h &ӷ M&S% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b & ( +142 h ( M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &z' +93 h '  .t\Elbow.hold M& , +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %  A(~& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\ ' +23 h ' A( % h %Z ."downElevator"\SE.seek :down A('X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'" { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'*& h & .tHand.open M&1 +2 h 1 M&+& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&((- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&%/ +150 h / M&,& h &- M&1% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +264 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'3(W +416 h (W C'6& h &W .tHand.open M&51 +150 h 1 M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%7 +150 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%=' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%;  +38 h  .t\PS.seek 0ml P%>' +1597 h ' .t\PSV.dial PSR P%4 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%A' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%@  C'C  h +75 .t\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%F  +113 h .t\PS.seek 0ml P%G' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%E' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%H' +2610 h ' .t\PSV.dial :air P%K  +38 h  .t\PS.seek 2.5ml P%J' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%D' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&?& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&L' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C'-( +416 h ( M&M& h &ӷ M&&% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&V1 +150 h 1 M&U% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'X& h & M&!% h %\  A([% h %X A(^. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (]( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (#& h & M&Y& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &e( +166 h ( M&f& h & A(a& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &b' +89 h ' A(W& h & .t\SE.seek 19.71 Pucks below Top Plate A(Z' +66 h ' A(l& h & M&o& h & .t\Elbow.seek 3 M&c( +6 h ( M&\\& h & A(t& h & .t\Forearm.seek 3 + 300 counts M&_'B +23 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &`/ +150 h / ."downElevator"\SE.seek :down M&I& A(g'X h & M&x% h %B .t\Forearm.seek :retract M&d'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&q(ӷ +142 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &P' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&j1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'm  +150 h  .t\CSV.dial CTV C'w  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'|' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'u'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%S  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%s  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%i +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C'~ @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C' @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C'z'4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'B& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&% h %\  A("% h %X A(*. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (+& h &  M&& h &ӷ M&\ % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +89 h ' A(k& h &  .t\SE.seek -0.29 Pucks below Top Plate A( '\\ +264 h '\\ M&%& h & A(,& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &1' +26 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(/' +27 h ' M&2% h %  A((& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&r& h & A(6& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &5' +23 h ' .t\SE.seek 1.04 Pucks below Top Plate A(8' +36 h ' M&7& h & M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &''\ +89 h '\  .t\Elbow.seek CC M&;(ӷ +143 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.seek CC M&>' +91 h ' .tHand.close M&@/ +150 h / .t\CC.seek :open C'C( +416 h ( A(:& h &  A(F& h &  M&A& h &ӷ M&% h % .t\Forearm.seek :retract M&G'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&E& h & A(K& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &H' +93 h '  .t\Elbow.hold M& , +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &J1 +150 h 1 M&?% h %  A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&)'8 +20 h '8 M&.& h & A(0& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &9' +22 h ' A(3% h % ."downElevator"\SE.seek :down A(M'X +100 (&% h %S M&L% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%T  + A(6 +112 h  P%$% h % .t\PS.seek 0.499ml P%V' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%U +38 h .t\PRV.dial :air P%N  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%X  +37 h .t\CBV.dial AIV C'!  +38 h .t\PRV.dial between CBV and air P%[  +19 h  .t\PRV.dial :air P%^  +19 h  P%#% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %Y' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %R' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'f& h & .tHand.open M&a1 +2 h 1 M&W& h & M&O% h %\  .t\Elbow.seek FlushPuck::Garage M&l(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &- .t\Forearm.seek :garage M&t'η +91 h 'η .tHand.close M&p/ +150 h / M&`& h &- M&I% h %η .t\Forearm.seek :retract M&x'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &e(ӷ +264 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &v' +91 h ' .t\CC.seek :closed C'_(W +416 h (W C'Z& h &W .tHand.open M&g1 +150 h 1 M&c% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &d'\ +91 h '\  C'-& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%j @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%m  +75 h  .tdelay 2 seconds +200 P%w% h % .t\PS.seek 1.499ml P%P' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%]' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%| +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%S  +38 h  .tdelay 2 seconds +200 P%D% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%s  +75 h .tdelay 2 seconds +200 P%i% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%~'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%u' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%B  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%" +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%*  +75 h P%+% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'\ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'k { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %q' +1313 h ' .t\PSV.dial :air P%n  +37 h  .t\PS.seek 2.5ml P%%' +2610 h ' .t\PSV.dial :PML P%, +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '2& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'(& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*r @@T .t\AWV.select :air F*6  .t\AIV.dial :bypass .\PSV.dial PSR P%7 +22 h  ."MFBPCR"delay 20 seconds +91 h P%<% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%}' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +128 h I F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *1'6 +66 .t\PSV.dial :waste1 P%=  +38 h  .t\PS.seek 0ml P%4' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%/' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%5 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +22 h ' .t\PSV.dial PSR P%' +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*:'ͯ +92 h P%F% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%A' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%K  +38 h  .t\PS.seek 0ml P%>' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*;  +22 h  F*J% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *?' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%.' +217 ."MFBPCR"\ATV.dial :AIV F*0 I +43 h I F*3% h % ."MFBPCR"\AS.seek 0.469ml F*G' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%&' +2610 h ' .t\PSV.dial PSR P%L +113 h P%H% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%)' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +37 h  .t\PS.seek 0ml P%T' +740 h ' ."MFBPCR"delay 5 seconds +500 F*$9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*9 n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*M% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%U' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%N +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial PSR P%! +113 h P%[% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%#  +38 h  .t\PS.seek 0ml P%V' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%a +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %W'k +2678 h 'k .t\PSV.dial :waste1 P%O  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %o' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*\\ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b * 'V +850 h ' .t\PSV.dial :air P%p  +113 h  .t\PS.seek 2.5ml P%`' +2609 h ' .t\PSV.dial :mixloop P%I +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %Q' +5107 h ' .t\PSV.dial :air P%t  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %b' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*{  +43 h  F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *x'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*C I +43 h I F*Z% h %Q ."MFBPCR"\AS.seek 0.01ml F*g' +171 h ' .t\PSV.dial :mixloop P%c +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %-' +618 h ' ."MFBPCR"\ATV.dial :waste F*j n +129 h n ."MFBPCR"\AS.seek :empty F*v'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +85 h  F*m% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *w' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*R  +43 h F*P% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C']  +38 h  C'|% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'S'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'D' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  's  +37 h  +113  .t\CBV.dial :waste1 C'i @ +38 h @ .t\CS.seek 0ml C''4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'  +38 h  C'~% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F* % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*u n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F*'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'B' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'"'4 +2648 h '4 C'*& h &W #tGaraging FlushPuck in Collection Clamp M&+& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&k/ +150 h / .t\CC.seek :open C'( +416 h ( M&n& h &ӷ M&q% h % .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &%(- +264 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&71 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'& h & M& % h %\  A(=% h %X A(h. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (}( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&4& h &- M&5% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &/( +166 h ( M&@& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &'' +90 h ' A(F& h & ."WCR"\SE.seek 18.71 Pucks below Top Plate A(1' +77 h ' A(& h & M&& h & ."WCR"\Elbow.seek 3 M&:( +6 h ( M&K& h & A(;& h & ."WCR"\Forearm.seek 3 + 300 counts M&J'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +150 h / ."downElevator"\SE.seek :down M&E& A('X h & M&0% h %B ."WCR"\Forearm.seek :retract M&3'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&>(ӷ +143 h (ӷ M&?& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +91 h ' ."WCR"\CC.seek :closed C'L(W +3 ."MFBPCR"\ATV.dial :waste2 F*H % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +370 h (W ."WCR"Hand.open M&81 +150 h 1 M&)% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*9  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *M' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)G+ +6 h + S)% h % C'T& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'N  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +38 h @ C'$% h %0 .t\CS.seek 5ml C'X'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'^'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C'W +75 h .t\CS.seek 0ml C' '4 +507 h '4 #tDisinfecting external Intake with mfbkill C'\\& h &W C'Y% h %4 .t\CSV.dial CSR C'`  +150 h  C'{% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'!'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'}' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'o' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'C  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'f'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'O  +113 h  C'p% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'V'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Q  'L  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'l'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'M  +37 h  C'j% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'R'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Z  +37 h  .t\CBV.dial :waste2b C'I  +76 h  .t\CS.seek 0ml C'|'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'v  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ']'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'z  +38 h +75 .t\CTV.dial :air C'g  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'~'4 +506 h '4 #t\Flushing upper manifold C' & h &W C' % h %4 .t\CSV.dial :flush C'm  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C't @ +38 h @ C'D% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'T'0 +506 h '0 .t\CSV.dial :waste C'w +38 h .t\CS.seek 0ml C''4 +506 h '4 C'u& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +37 h @ C'"% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'+'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'x'0 +506 h '0 .t\CSV.dial :waste C'_ +75 h .t\CS.seek 0ml C'n'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'd& h &W C'2% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'7 @ +38 h @ C'e% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C',  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'<'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'h& h &W C'% h %4 .t\CSV.dial :flush C'=  +75 h  .t\CS.seek 5ml C'('p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'4% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +37 h  .t\CBV.dial :waste2b C'F  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'% h %4 .t\CSV.dial CSR C';  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'%' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A 'K  +38 h +75 .t\CTV.dial :intake C''  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'0' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'/  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'J'4 +607 h '4 .t\CSV.dial CSR C'E  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'?' +1372 h ' .t\CSV.dial CBV C'1 +113 h .t\CS.seek 0ml C'H'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&3& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&)& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&9' +91 h ' .tHand.close M&N/ +150 h / .t\CC.seek :open C'8( +416 h ( M&&& h &ӷ M&.% h % .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&>& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&X1 +150 h 1 M&^% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +90 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'a& h & .tHand.open M&\\1 +2 h 1 M&Y& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &`(- +7 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&!'η +91 h 'η .tHand.close M&#/ +150 h / M&& h &- M&}% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &o(ӷ +264 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &f' +91 h ' .t\CC.seek :closed C'V(W +416 h (W C'Q& h &W .tHand.open M&L1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'M& h &W C'j% h %4 .t\CSV.dial CSR C'l  +113 h  C'R% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'Z'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'P'[ +463 h '[ .t\CSV.dial :waste C'v +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'S'4 +2010 h '4 #t\Purging external Intake C'|& h &W C']% h %4 .t\CSV.dial :flush C'z  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'g +76 h .t\CTV.dial :intake C'i  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C's& h &W C' % h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'm'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +38 h @ C't% h %p .t\CS.seek 7ml C'D'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'w'4 +506 h '4 C'T& h &W C'u% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'*% h %0 .t\CS.seek 5ml C'"'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'+ +75 h .t\CS.seek 0ml C'_'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&x& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +7 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&n' +90 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'e( +416 h ( M&& h &ӷ M&,% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&=1 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +91 h '\  #t\Completed [coreNEG] begun 14:00:15 #\Preparing to sleep until 14:00 19-oct-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00 19-oct-18 #\Continuing at 14:00CEST19-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:122\\n{1 => 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:20:04, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST19-Oct-18" +279951 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1815h.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +21 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*/  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +65 h  F*E% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +31 h  ."MFBPCR"\ATV.dial :air3 F*N % +87 h % F*\ % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*&% h % ."MFBPCR"\AS.seek 0.81ml F*.'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*$'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*X% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*[  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *#'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *W'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1817h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*!  +42 h  ."MFBPCR"\ARV.dial :carrier F*O  +11 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*`'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*M  +22 h ."MFBPCR"delay 2 seconds +200 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *l'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*R  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*f% h %Fj ."MFBPCR"\AS.seek 0.524ml F*H' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*I  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*v' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.536ml F*]'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*z  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*g'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*i n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*s  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*~  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*m'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*D % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1817h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F**'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*2  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*q  +64 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *o'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*h  +32 h  ."MFBPCR"\ATV.dial :air3 F*d % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *<' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*r'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*5% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1820h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*J% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*? n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*N  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\  +22 h ."MFBPCR"delay 2 seconds +200 F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*& I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*k% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*> % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*X  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*['< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +22 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*\\'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +22 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*G  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*#% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*}  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*!% h % \\ ."MFBPCR"\AS.seek 0.326ml F*O'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1820h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*` @@T ."MFBPCR"\ASV.dial :SPEdelivery F*8  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*Q  +151 h F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *M' +423 h ' ."MFBPCR"\ATV.dial :ASV F*j n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*L  +43 h  F*R% h % ."MFBPCR"\AS.seek 0.62ml F*f'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*l n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *Z'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*H'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*P % +43 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *v' +423 h ' ."MFBPCR"\ATV.dial :air3 F*z% h % ."MFBPCR"\AS.seek 0.53ml F*S' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +21 h F*i% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h % H ."MFBPCR"\AS.seek 0.735ml F* ' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*~' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F* % h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*D n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*m% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*u% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*"% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F**' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*+ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*7 % +108 h % F*x% h %V ."MFBPCR"\AS.seek 0.01ml F*o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +22 h F*V% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h % q ."MFBPCR"\AS.seek 0.605ml F*h'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*=% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*<'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *B'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*r  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *F' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*5 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *@'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +202 h 'R ."MFBPCR"\SPE.coast F*;  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F* n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *A' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*' I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *K'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*0  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*J  +64 h  F*E% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*? n +65 h n F*1% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *3' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*N I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *)'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*:  +43 h  F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *k': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*>% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *.'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*9 n +65 h n F*X% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *$' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*a  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *[': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*Y n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *{'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*\\ n +151 h n ."MFBPCR"\AS.seek 0ml F*G'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*#  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*}% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*p'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %0 ."MFBPCR"\AS.seek 0.985ml F*O' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F* % h % ."MFBPCR"\AS.seek 0.97ml F*C'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*j I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *R'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *l'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *I'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*Z  +43 h  F*P% h %R ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.009ml F*|' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *H' +9988 h ' ."MFBPCR"\ATV.dial AIV F*z I +43 h I ."MFBPCR"\AS.seek 0.02ml F*v' +9880 h ' ."MFBPCR"\ATV.dial :waste F*] n +129 h n ."MFBPCR"\AS.seek 0ml F*i'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*S% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*s  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*m% h %n ."MFBPCR"\AS.seek 0.82ml F*U'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*T  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F** % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +21 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*n% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *='R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*; n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *@'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*( % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast P%6 ."shutdown"\PS.coast .\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539950400.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* *' h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *A  h  .t\SPE.configure SPEconfig F*\ 2 *K% h %V .t\AS.configure ASconfig S)0% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'%& S)#.0X% h %= .t\Forearm.reconfigure LooseForearm .\Forearm.home.seek :retract M&#@2^d^2b &k'\ h ."rdySampler"\CSV.dial :waste1 C'9 h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S)X h  S).  +38 h  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +20 h '\  ."closeHand"Hand.close M&$/ .t\Elbow.jog 1500 M&a* +17 h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml C'^'4 S)#.0X 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:20:46CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&O1 +150 h 1 M&j& h &M M&C% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &L(- +166 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &l'η +91 h 'η .tHand.close M&f/ +150 h / M&Z& h &- M&P% h %η .t\Forearm.seek :retract M&I'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +264 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &]' +91 h ' .t\CC.seek :closed C'S(W +416 h (W C'v& h &W .tHand.open M&i1 +150 h 1 M&s% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'g% h %4 .t\CSV.dial CSR C'  +150 h  C't% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '~% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'D'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Purging external Intake C'm& h &W C'% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'u' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U '  h +75 .t\CTV.dial :intake C'w  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C''4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'"% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +37 h @ C'% h %p .t\CS.seek 7ml C'+'i +416 h 'i ."connect"\CSV.dial CBV C'7 .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'x'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'2'0 +507 h '0 .t\CSV.dial :waste C'c +37 h .t\CS.seek 0ml C'V'4 +507 h '4 C'q& h &W C',% h %4 .t\CSV.dial :flush C'n  +38 h  .t\CS.seek 3ml C'o'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'd @ +38 h @ C'% h %0 .t\CS.seek 5ml C'='p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'h'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &4(ӷ +7 h (ӷ M&r& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &ӷ .t\Forearm.seek CC M&5' +91 h ' .tHand.close M&F/ +150 h / .t\CC.seek :open C';( +416 h ( M&& h &ӷ M&(% h % .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&-& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&y1 +150 h 1 M&b% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:20:51CEST18-Oct-18\\n{1 => 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:20:46CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*6% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'`& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b ('( +6 F*A% h %&K ."readyMFB"\ATV.dial :waste2 F*K % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *0' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (%& h & M&/& h &- M&J% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &E( +166 h ( M&& h & A(?& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &1' +90 h ' A(& h & .t\SE.seek 17.71 Pucks below Top Plate A(N'* +86 h '* A(3& h & M&\ & h & .t\Elbow.seek 3 M&:( +7 h ( M&&& h & A(>& h & .t\Forearm.seek 3 + 300 counts M&)'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &9/ +150 h / ."downElevator"\SE.seek :down M&& A(X'X h & M&.% h %B .t\Forearm.seek :retract M&$'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&\\(ӷ +142 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'W(W +416 h (W .tHand.open M&81 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  .t\SC.coast #collectSample 300ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +7 h + S)G% h % C' & h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +100 ."CSpush"\CSV.dial :air C' @ +38 h @ ."CSpush"\CS.reconfigure CSfast .\CS.seek 0ml C'#d @b '9'4 +6 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S) +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'"9 h 9Td +100 C'u9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'79 h 9d +100 C'+9 h 9d +100 C'x9 h 9 d +100 C'9 h 9 d +100 C'9 h 96d +100 C'29 h 9Ld +100 C'q9 h 9bd +100 C',9 h 9xd +100 C'*9 h 9d +100 C'V9 h 9d +100 C'd9 h 9d +100 C'9 h 9d +100 C'n9 h 9d +100 C'o9 h 9d +100 C'h9 h 9d +100 C'9 h 9(d +100 C'<9 h 9>d +100 C'=9 h 9Td +100 C'B9 h 9id +100 C'c9 h 9d +100 C'r9 h 9d +100 C'9 h 9d +100 C'F9 h 9d +100 C'9 h 9d +100 C'S9 h 9d +100 C'(9 h 9  d +100 C'-9 h 9 d +100 C'|9 h 9 *d +100 C'59 h 9 ?d +100 C'_9 h 9 Td +100 C'y9 h 9 j d +100 C'9 h 9 $d +100 C'49 h 9 (d +100 C'@9 h 9 ,d +100 C'69 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9 9 h 9 d +100 C'.9 h 9 d +100 C'E9 h 9 d +100 C'19 h 9 d +100 C'{9 h 9 d +100 C'!9 h 9 d +100 C'a9 h 9 d +100 C')9 h 9 d +100 C'N9 h 9 1d +100 C'X9 h 9 Dd +100 C'$9 h 9 Xd +100 C':9 h 9 kd +100 C'89 h 9 ~d +100 C';9 h 9 d +100 C'G9 h 9 d +100 C' 9 h 9 d .t\CH.hold 5:00,85C C'k   P h  +30000  C''9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C' @ +113 h @ .t\CSV.dial :air C'Y @ +37 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'R  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'M @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'O  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'0 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'Z'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'P +113 h .t\CBV.dial CSV C'  +19 h C'}% h %4 .t\CS.seek 5ml C'z'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'H  +38 h C'L% h %p .t\CS.seek 7ml C'I'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'Q @ +38 h @ .t\CS.seek 10ml C'l' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C's ` +132 h ` #tLysing Complete C'& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&g% h %\  A( % h %X A(v. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (i( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (]& h &  M&f& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M& & h & A(D& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &m' +90 h ' A(& h &  .t\SE.seek 1.71 Pucks below Top Plate A(T': +244 h ': M&t& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &U' +25 h '  .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(w- h -+: .t\SE.seek 2.04 Pucks below Top Plate A(' +27 h ' M&"% h %  A(u& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(7' +36 h ' M&+& h & M&x% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&,' +91 h ' .tHand.close M&*/ +150 h / .t\CC.seek :open C'V( +416 h ( A(d& h &  A(& h &  M&n& h &ӷ M&o% h % .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&<& h & A(=& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &B' +93 h '  .t\Elbow.hold M&c, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &r1 +150 h 1 M&% h %  A(F& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'8 +21 h '8 M&S& h & A((& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &-' +22 h ' A(|% h % ."downElevator"\SE.seek :down A(5'X +100 (_% h %1 M&y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +42 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'4 @ +2 A(6 +17 h @ .t\CS.seek 10ml C'@' +7 h ' .t\CSV.dial between air and CTV C'6 ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&A& h & M&K% h %\  .t\Elbow.seek FlushPuck::Garage M&b(- +165 h (- M&/& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.seek :garage M&e'η +91 h 'η .tHand.close M&`/ +150 h / M&& h &- M&?% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\ & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &&' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'3& h &W .tHand.open M&1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &9'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%> +150 h P%.% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%E' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%{' +1598 h ' .t\PSV.dial PSR P%! +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%a' +2609 h ' ."connect"\PRV.dial CBV P%)  .t\CBV.dial PRV C'N  h +75 .t\PSV.dial :mixloop P%X +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%$  h .t\PS.seek 0ml P%:' +2610 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.seek 2.5ml P%;' +2609 h ' .t\PSV.dial :mixloop P%G +38 h .t\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial :air P%k  +37 h  .t\PS.seek 2.5ml P%'' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P% ' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.seek CC M&O' +91 h ' .tHand.close M&0/ +150 h / .t\CC.seek :open C'( +416 h ( M&P& h &ӷ M&% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Z(- +264 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&j% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C's& h & M&% h %\  A(g% h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (v( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (]& h & M&f& h &- M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +166 h ( M&D& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\\' +90 h ' A([& h & .t\SE.seek 16.71 Pucks below Top Plate A(t'; +95 h '; A(& h & M&w& h & .t\Elbow.seek 3 M&"( +7 h ( M&u& h & A(& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down M&m& A(x'X h & M&% h %B .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.seek CC M&U(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&2& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'W  +19 h  .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'n'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%o  +38 h  .t\PS.seek 2.5ml P%<' +2610 h ' .t\PSV.dial :mixloop P%= +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%,  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%r  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C'F @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'S  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'( @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'|  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'h ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C'_' +2647 h ' .t\CSV.dial CTV C'y  +38 h  .t\CS.seek 0ml C'4'4 +2647 h '4 .t\CBV.dial :waste1 C'B @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'6  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&% h %\  A(% h %X A(A. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (K( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&7& h &ӷ M&-% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &/( +142 h ( M&J& h & A(i& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &5' +90 h ' A(l& h &  .t\SE.seek 2.71 Pucks below Top Plate A(c') +234 h ') M&`& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &?' +26 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(e- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A( ' +27 h ' M&\ % h %  A(b& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'8 +20 h '8 M&V& h & A(3& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(%' +37 h ' M&>& h & M&.% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &&'\ +89 h '\  .t\Elbow.seek CC M&1(ӷ +142 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&{' +91 h ' .tHand.close M&)/ +150 h / .t\CC.seek :open C'N( +416 h ( A(X& h &  A($& h &  M&a& h &ӷ M&8% h % .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &G( +143 h ( M&;& h & A(k& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b & ' +92 h '  .t\Elbow.hold M&C, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &'1 +150 h 1 M&Y% h %  A(R& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&M'8 +21 h '8 M&0& h & A(#& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &9' +22 h ' A(% h % ."downElevator"\SE.seek :down A(P'X +100 (% h %  M&O% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%L  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%Q' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%j +38 h .t\PRV.dial :air P%}  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%s  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%g  +19 h  .t\PRV.dial :air P%  +19 h  P%]% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %f' +250 h ' .t\PRV.dial :waste2 P%~ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %z' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&1 +2 h 1 M&[& h & M&Z% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&I/ +150 h / M&+& h &- M&m% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C't(W +416 h (W C'& h &W .tHand.open M&"1 +150 h 1 M&x% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%W% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%v' +1094 h ' .t\PSV.dial :air P%@  +38 h  .t\PS.seek 0ml P%d' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P% +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%o  +38 h  .tdelay 2 seconds +200 P% % h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %='r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%,  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%r' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%<  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%F'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%S  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%(  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %|' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%h +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%n' +2609 h ' .t\PSV.dial :air P%y  +76 h  .t\PS.seek 2.5ml P%_' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%B +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%6' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%A  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %7' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'- & +50 h & .t\AWV.select :waste C' +7 P%J% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %i'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'l { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %U' +1313 h ' .t\PSV.dial :air P%*  +37 h  .t\PS.seek 2.5ml P%`' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%e' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '\ & h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'b& F*V @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*3  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%> +22 h  ."MFBPCR"delay 20 seconds +91 h P%.% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%5' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*/ I +128 h I F*c% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *?'6 +66 .t\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%!' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*)  +22 h ' .t\PSV.dial PSR P%& +21 h  F*% h %6 ."MFBPCR"\AS.seek 0.329ml F*X'ͯ +92 h P%$% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%a' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*8 % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *;'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%k  +38 h  .t\PS.seek 0ml P%{' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*1  +22 h  F*'% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *Y' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%R +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%0' +217 ."MFBPCR"\ATV.dial :AIV F*# I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*:' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%G +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%O +113 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%M' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +37 h  .t\PS.seek 0ml P%L' +740 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*9 n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*P% h % ."MFBPCR"\AS.seek 0.041ml F*K'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%j' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +2610 h ' .t\PSV.dial PSR P% +113 h P%g% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%]  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%~ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%f' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %['k +2678 h 'k .t\PSV.dial :waste1 P%Z  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %w' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*u I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *C'V +850 h ' .t\PSV.dial :air P%I  +113 h  .t\PS.seek 2.5ml P%+' +2609 h ' .t\PSV.dial :mixloop P%m +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %H' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\\' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*2  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*N I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*"' +171 h ' .t\PSV.dial :mixloop P%x +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*q'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +85 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *W' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*z  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *@'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'v  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'o'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C',  'd  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'='4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*<  +21 h ."MFBPCR"delay 3 seconds +300 F*r% h %_ ."MFBPCR"\AS.seek 0.434ml F*S'ݏ +110 h '4 .t\CSV.dial CSR C'(  +38 h  C'F% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'h'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F*|% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*y'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*n n +108 h n F*B% h %& ."MFBPCR"\AS.seek 0.092ml F*_'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'6  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2648 h '4 C'A& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&-& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&7' +91 h ' .tHand.close M&l/ +150 h / .t\CC.seek :open C'i( +416 h ( M&*& h &ӷ M&U% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &`(- +264 h (- M&\ & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &3'η +91 h 'η .tHand.open M&>1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &e'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'/& h & M&c% h %\  A(E% h %X A(^. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (5( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&!& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +165 h ( M&)& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A($& h & ."WCR"\SE.seek 15.71 Pucks below Top Plate A(?'L +106 h 'L A(& h & M&8& h & ."WCR"\Elbow.seek 3 M&X( +7 h ( M&k& h & A(1& h & ."WCR"\Forearm.seek 3 + 300 counts M&''B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &a/ +148 h '&0 ."MFBPCR"delay 3 seconds +2 h / ."downElevator"\SE.seek :down A(;'X M&R& h & M&#% h %B ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.seek CC M&{(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&Y& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +64 ."MFBPCR"\ATV.dial :waste2 F*O % +27 h ' ."WCR"\CC.seek :closed C' (W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +399 h (W ."WCR"Hand.open M&%1 +150 h 1 M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +11 h 'R ."MFBPCR"\ATV.dial :air2 F*9  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *P' +59 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S):+ +7 h + S)% h % C'L& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'}  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % k ."MFBPCR"\AS.seek 0.779ml F*.'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b */' +4416 h ' ."MFBPCR"\ATV.dial :waste F*c n +129 h n ."MFBPCR"\AS.seek :empty F*E'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*^ % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*3% h % ."MFBPCR"\AS.seek 0.579ml F*)' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*$% h %  ."MFBPCR"\AS.seek 0.604ml F*`'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*8% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*1 n +86 h n ."MFBPCR"\ASV.dial :wasteR F*e  +43 h  ."MFBPCR"\AS.seek 0ml F*a'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *#'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*&% h %V ."MFBPCR"\AS.seek 0.59ml F*Y' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*G n +107 h n ."MFBPCR"\ASV.dial :wasteR F*'% h %  ."MFBPCR"\AS.seek 0.09ml F*?' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*X I +86 h I F*;% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*i % +21 h % F*_% h % ."MFBPCR"\AS.seek 0.13ml F*%' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +21 h F*9% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*}% h % ."MFBPCR"\AS.seek 0.255ml F*0'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*5% h %X ."MFBPCR"\AS.seek 0.24ml F*:' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*g n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *]% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b * '5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*j @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*K I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*~'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Z  +43 h  #"MFBPCR"\Swish #1 of 2 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *u'\  +1031 h '\  F*D% h %\  ."MFBPCR"\AS.seek 0.5ml F*I'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*f% h %(A ."MFBPCR"\AS.seek 0.6ml F*+'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*H'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*2% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *w' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*m % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +3472 h 'V ."MFBPCR"\SPE.coast F*N  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1915h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*x  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.reconfigure ASnorm !to:SignalException :\ signoi:bt[ "1/opt/mbari/lib/ruby/1.8/irb.rb:72:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" SIGHUP:@checkpoint: omitted ."MFBPCR"\AS.seek :empty F*#,,'T20 b !to:Errno::EIO :bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error:@timestampu: Time mu@ F*'R !to:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error .\AS.stop !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" execute '18tundeployv4.rb' .GoodNews.suppressMail "Starting 18tundeployv4 w/pucks in tube 3\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:24:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tundeployv4 begun" #\Preparing to sleep until 14:00 18-oct-18 #"shutdown"\Shutdown #t\Asleep til 14:00 18-oct-18 #\Continuing at 14:00CEST18-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: coreNEG\\n/home/esp/esp2/mission/18tundeployv4.rb:118\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:25:12, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 14:00CEST18-Oct-18" @CEST1539864000.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:25:17, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &M M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C'Q .t\CBV.dial CSV C'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV C'] .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:25:22CEST18-Oct-18\\n{1 => 0, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:25:17, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +65 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M& & h &- M&\ % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +166 h ( M& & h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h & .t\SE.seek 20.71 Pucks below Top Plate A(' +56 h ' A(& h & M&& h & .t\Elbow.seek 3 M&( +7 h ( M&& h & A(& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %B .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)'% C'/'4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +1090 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C'99 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'[9 h 9d +100 C'U9 h 9  d +100 C'Z9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A(9'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +18 h @ .t\CS.seek 10ml C'J' +6 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&[% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +265 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +90 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +37 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +38 h  .t\PS.seek 2.5ml P%o' +2609 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2610 h ' .t\PSV.dial :air P%u  +37 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +38 h .t\PS.seek 0ml P%n' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +91 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C' & h & M&z% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (w& h & M& & h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A( & h & .t\SE.seek 19.71 Pucks below Top Plate A(' +65 h ' A(& h & M&#& h & .t\Elbow.seek 3 M&( +7 h ( M&& h & A((& h & .t\Forearm.seek 3 + 300 counts M&'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&s& A('X h & M&,% h %B .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +143 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +90 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C')'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2610 h ' .t\PSV.dial :mixloop P%: +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +150 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +18 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'l& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (M( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (S& h &  M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +142 h ( M&P& h & A(9& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &1' +90 h ' A(& h &  .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +264 h '\\ M&W& h & A(T& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &[' +25 h '  .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +27 h ' M&\\% h %  A(Q& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&U'8 +21 h '8 M&&& h & A(`& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +90 h '\  .t\Elbow.seek CC M&e(ӷ +142 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h &  A(p& h &  M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &C( +143 h ( M&o& h & A(u& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &t' +92 h '  .t\Elbow.hold M&?, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %  A(q& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&{'8 +21 h '8 M&~& h & A(Z& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P%\ ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'z  +38 h .t\PRV.dial between CBV and air P%  +18 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb % ' +251 h ' .t\PRV.dial :waste2 P%} @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M&$/ +150 h / M&& h &- M&s% h %η .t\Forearm.seek :retract M&,'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +37 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +37 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +75 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%)' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%l  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +75 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %9'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1312 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2610 h ' .t\PSV.dial :PML P%T +37 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*& @@T .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *['6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1598 h ' .t\PSV.dial PSR P%U +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%Y' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*Z I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P% ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P%z +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +37 h  .t\PS.seek 0ml P%\ ' +662 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%} +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%$  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%s +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5106 h ' .t\PSV.dial :air P%(  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +170 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F**% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +37 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'! @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +22 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +37 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'6'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*) n +107 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'l' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'9( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'L& h & M&@% h %\  A(g% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (1( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h & M&^& h &- M&_% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Y( +166 h ( M&j& h & A(4& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(p& h & ."WCR"\SE.seek 18.71 Pucks below Top Plate A([' +77 h ' A(K& h & M&;& h & ."WCR"\Elbow.seek 3 M&d( +7 h ( M&u& h & A(e& h & ."WCR"\Forearm.seek 3 + 300 counts M&x'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &k/ +150 h / ."downElevator"\SE.seek :down M&o& A(q'X h & M&Z% h %B ."WCR"\Forearm.seek :retract M&]'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' ."WCR"\CC.seek :closed C'v(W +3 ."MFBPCR"\ATV.dial :waste2 F*t % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&b1 +150 h 1 M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +7 h + S)8% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *E' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*)% h % ."MFBPCR"\AS.seek 0.279ml F*D'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*H n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*G % +43 h % F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *R'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*S I +22 h I F*=% h %v ."MFBPCR"\AS.seek 0.059ml F*P'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*"% h % ."MFBPCR"\AS.seek 0.769ml F*%' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +21 h % ."MFBPCR"delay 3 seconds +300 F*\\% h % q ."MFBPCR"\AS.seek 0.794ml F*Q' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*a% h % k ."MFBPCR"\AS.seek 0.779ml F*f'  +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*T I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *L' +4416 h ' ."MFBPCR"\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.seek :empty F*g'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*U% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*`% h % ."MFBPCR"\AS.seek 0.579ml F*j' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %  ."MFBPCR"\AS.seek 0.604ml F*W'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*;% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*V  +43 h  ."MFBPCR"\AS.seek 0ml F*k'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Z'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*Y % +108 h % F*X% h %V ."MFBPCR"\AS.seek 0.59ml F*i' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*C n +108 h n ."MFBPCR"\ASV.dial :wasteR F*x% h %  ."MFBPCR"\AS.seek 0.09ml F*[' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*q I +85 h I F*d% h % ."MFBPCR"\AS.seek 0.12ml F*]' +626 h ' ."MFBPCR"\ATV.dial :air3 F*9 % +21 h % F*I% h % ."MFBPCR"\AS.seek 0.13ml F*b' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*c% h % ."MFBPCR"\AS.seek 0.23ml F*8' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*~'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*1% h %X ."MFBPCR"\AS.seek 0.24ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*z  +22 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *w% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6659 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*M I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*$'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F* % h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F*(% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*'% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *#' M +3723 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*s % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +3472 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1815h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*+ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*0% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*.' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*!  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.536ml F*/'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*F% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*J'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*E  +43 h  ."MFBPCR"\ASV.dial :waste1 F*H  +65 h ."MFBPCR"\ARV.dial :air1 F*D  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*l'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*S % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226124 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'=  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'R @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)P% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'"% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'B' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\\  '%  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'N'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'a  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Q' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'T  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'f  +38 h  C'@% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'U' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'L @ +75 h @ +50 S)g% h % ."WCR"\CBV.dial between CSV and waste2b C'_  +94 h  C'`% h % ."WCR"\CSV.dial between air and CTV C'^ ` +57 h ` ."WCR"\CS.seek 7.288ml C'4' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'p  ."WCR"\CSV.dial CTV C'j  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C';  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'e  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'V  +38 h  C'u% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'k' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'o' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'Y @ +75 h @ +50 S)X% h % ."WCR"\CBV.dial between CSV and waste2b C'Z  +95 h  C'C% h % ."WCR"\CSV.dial between air and CTV C'x ` +56 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C't  'q  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'[  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '9' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'I  +151 h C']& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&{% h %\  A(c% h %X A(b. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&8& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &1( +142 h ( M&~& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &z' +90 h ' A(r& h &  ."WCR"\SE.seek 0.71 Pucks below Top Plate A(w'K +253 h 'K M& & h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &M' +26 h '  ."WCR"\Elbow.hold M&\ , +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M& % h %  A(}& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&& h & A( & h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &$' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A((' +37 h ' M&& h & M&'% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&s(ӷ +142 h (ӷ M&#& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C',( +416 h ( A(?& h &  A(& h &  M&& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&5'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&& h & A(*& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +92 h '  ."WCR"\Elbow.hold M&+, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &01 +150 h 1 M&% h %  A(& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&7'8 +21 h '8 M&& h & A(!& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &n' +22 h ' A(.% h % ."downElevator"\SE.seek :down A(A'X +100 (F% h %B M&/% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +90 h '\  +51 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C':& h & .tHand.open M&21 +2 A(6 h 1 M&>& h & M&<% h %\  .t\Elbow.seek FlushPuck::Garage M&J(- +166 h (- M&E& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.seek :garage M&D'η +91 h 'η .tHand.close M&G/ +150 h / M&)& h &- M&6% h %η .t\Forearm.seek :retract M&S'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &=(ӷ +264 h (ӷ M&R& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'P(W +416 h (W C'& h &W .tHand.open M&"1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'%& h &W C'\\% h % .t\CSV.dial CSR C'B  +38 h  C'a% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'N'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'T% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +462 h '[ .t\CSV.dial :waste C'@ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'Q'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'L% h %4 .t\CSV.dial :flush C'g  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '_'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'^% h %p .t\CS.seek 7ml C'U'i +416 h 'i ."connect"\CSV.dial CTV C'j  .t\CTV.dial CSV C'p  +38 h  +113  .t\CBV.dial :waste1 C'4 @ +75 h @ .t\CS.seek 0ml C';'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'e% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'u'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +37 h @ C'k% h %p .t\CS.seek 7ml C'Y'i +416 h 'i ."connect"\CSV.dial CTV C'X  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'Z  +75 h  .t\CS.seek 0ml C'C'4 +766 h '4 #t\Flushing upper manifold C'x& h &W C'o% h %4 .t\CSV.dial :flush C'q  +75 h  .t\CS.seek 5ml C't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'i @ +37 h @ C'[% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] 'I  +38 h +75 .t\CTV.dial :air C'{  +151 h .t\CS.seek 0ml C'c'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'8'4 +507 h '4 C'& h &W C'~% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'r'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +37 h @ C'y% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C'}'4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C' % h %4 .t\CSV.dial CSR C'  +151 h  C''% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'z'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'1' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'w +113 h .t\CTV.dial :intake C'M  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'#' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'm  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'$% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C'\ 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'v  +38 h  +113  .t\CBV.dial :waste1 C'? @ +38 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +709 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C's  +75 h  .t\CS.seek 0ml C'0'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +710 h ' ."connect"\CSV.dial CBV C'. .t\CBV.dial CSV C'5  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'F'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'/'0 +507 h '0 .t\CSV.dial :waste C': +37 h .t\CS.seek 0ml C'2'4 +507 h '4 #t\Flushing upper manifold C'>& h &W C'<% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'7'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'( @ +37 h @ C'n% h %p .t\CS.seek 7ml C'E'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C')& h &W C'6% h %4 .t\CSV.dial :flush C'3  +38 h  .t\CS.seek 3ml C'D'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'O% h %0 .t\CS.seek 5ml C'J'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'S'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C','0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'"'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'%% h %4 .t\CSV.dial :flush C'\\  +37 h  .t\CS.seek 5ml C'B'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'a @ +38 h @ C'% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'T  'N  +38 h  +113  .t\CBV.dial :waste1 C'@ @ +37 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'L% h %4 .t\CSV.dial :flush C'g  +76 h  .t\CS.seek 5ml C'Q'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +37 h @ C'^% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +38 h  .t\CBV.dial :waste2b C'p  +75 h  .t\CS.seek 0ml C'4'4 +766 h '4 #tDisinfecting external Intake with kill C'U& h &W C'K% h %4 .t\CSV.dial CSR C'e  +38 h  C'V% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C';'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'W' +416 h ' ."connect"\CSV.dial CBV C'k .t\CBV.dial CSV C'u  +37 h +75 .t\CTV.dial :intake C'X  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'Z' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'Y  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'x'4 +606 h '4 .t\CSV.dial CSR C'o  +113 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'C'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'i' +1372 h ' .t\CSV.dial CBV C'[ +113 h .t\CS.seek 0ml C't'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&I& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &](ӷ +7 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&c' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'b( +416 h ( M&& h &ӷ M&~% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &8(- +264 h (- M&h& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &r'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.seek :garage M&z'η +91 h 'η .tHand.close M&w/ +150 h / M&M& h &- M&1% h %η .t\Forearm.seek :retract M&m'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &#(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'\ (W +416 h (W C'& h &W .tHand.open M&v1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 's% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '*'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C'0& h &W C'% h %4 .t\CSV.dial :flush C'5  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '.' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'!  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'F'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C':  +75 h #t\Flushing upper manifold C'/& h &W C'>% h %4 .t\CSV.dial :flush C'<  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +37 h @ C'(% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 +113 h .t\CTV.dial :air .\CS.seek 0ml C'H'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'+'4 +507 h '4 C'& h &W C')% h %4 .t\CSV.dial :flush C'6  +38 h  .t\CS.seek 3ml C'3'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +38 h @ C'R% h %0 .t\CS.seek 5ml C'O'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'D'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'S +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&,& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&"' +91 h ' .tHand.close M&a/ +150 h / .t\CC.seek :open C'( +416 h ( M&B& h &ӷ M&T% h % .t\Forearm.seek :retract M&N'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&g1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +91 h '\  #t\Completed [coreNEG] begun 14:00:16 #\Preparing to sleep until 14:00 19-oct-18 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00 19-oct-18 #\Continuing at 14:00CEST19-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:122\\n{1 => 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:26:59, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST19-Oct-18" +279952 !"PCR"o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1815h.pcr F*^% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*j  +22 h ."MFBPCR"\ATV.dial :air3 F*p % +86 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *U'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*e% h %Z ."MFBPCR"\AS.seek 0.81ml F*V'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*u'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*W'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Z  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *q'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*d  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*~'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1817h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*z  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*$% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*b n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*?  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*t' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*s  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F** % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*0% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*:'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*/  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*>% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*F  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*(% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*n % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1817h.pcr F*H% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *6'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*R'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +21 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*D'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +21 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *#'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *f' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*j'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +21 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1820h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*W  +42 h  ."MFBPCR"\ARV.dial :carrier F*q  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *I'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *d'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*h % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*~% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*w% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*1  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*$  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1820h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *b  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*v  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *s'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F** % +43 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*5% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*!% h % ."MFBPCR"\AS.seek 0.71ml F*.' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*/% h % H ."MFBPCR"\AS.seek 0.735ml F*>' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*F% h % Ű ."MFBPCR"\AS.seek 0.72ml F*2' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*( n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*<% h % zG ."MFBPCR"\AS.seek 0.365ml F*7' +7162 h ' ."MFBPCR"\ATV.dial :waste F*n n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *E' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*)% h % ."MFBPCR"\AS.seek 0.579ml F*A' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %  ."MFBPCR"\AS.seek 0.604ml F*6'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*O% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*R' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*S n +85 h n ."MFBPCR"\ASV.dial :wasteR F*J  +22 h  ."MFBPCR"\AS.seek 0ml F*D'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *\\'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*a % +107 h % F*,% h %V ."MFBPCR"\AS.seek 0.01ml F*#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*\ % h % ."MFBPCR"\AS.seek 0.58ml F*B' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h % q ."MFBPCR"\AS.seek 0.605ml F* '\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*g% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*9' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*N n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*L  +22 h  ."MFBPCR"\AS.seek 0ml F*f'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *l'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*&  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *p' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *j'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +202 h 'R ."MFBPCR"\SPE.coast F*e  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*U'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*4 n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*;  +43 h  F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *k' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*X I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *u'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*Z  +43 h  F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *q': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*x  +65 h  F*o% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *C'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*i n +64 h n F*[% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *I' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *='V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*d  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*P  +65 h  F*h% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *~'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*c n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *y' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*] I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *''Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*r'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*w  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*} % +43 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*M% h % ."MFBPCR"\AS.seek 0.96ml F*$'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h % ."MFBPCR"\AS.seek 0.97ml F*m'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *s'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F**% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *t' +9988 h ' ."MFBPCR"\ATV.dial AIV F*5 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +128 h n ."MFBPCR"\AS.seek 0ml F*!'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*/  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*<'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +21 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*7'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*E% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*J  +31 h  ."MFBPCR"\ATV.dial :air3 F*R % +87 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*,% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*"% h %m ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *j'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*Q % +107 h % ."MFBPCR"\AS.seek 0ml F*4'V +2547 h 'V ."shutdown"\AS.coast F*5 ."shutdown"\SS.coast .\CC.coast S)5 C'6 ."shutdown"\CS.coast .\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 +2 C'5 P%5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1539950400.00 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ); h  C' 'V h  P% %U h  F* *X h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ C' 2\ P% 0\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*k  h  .t\SPE.configure SPEconfig F*\ 2 *u% h %V .t\AS.configure ASconfig S)Z% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'W& S)#.0X 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:27:39CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #2) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'?& h & .tHand.open M&1 +150 h 1 M&& h &M M&m% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &v(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&*% h %η .t\Forearm.seek :retract M&s'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +264 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&!1 +150 h 1 M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C':& h &W C'% h %4 .t\CSV.dial CSR C'>  +150 h  C'(% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'F'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'< +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'H'4 +2009 h '4 #t\Purging external Intake C'& h &W C'7% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ')' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  'E h +75 .t\CTV.dial :intake C'+  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'G'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'A  +75 h #t\Flushing upper manifold C'3& h &W C'O% h %4 .t\CSV.dial :flush C'6  +75 h  .t\CS.seek 5ml C'J'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +37 h @ C'D% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a +113 h .t\CTV.dial :air .\CS.seek 0ml C','4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'%'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'\ '4 +507 h '4 C'\\& h &W C'T% h %4 .t\CSV.dial :flush C'"  +38 h  .t\CS.seek 3ml C'#'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'B% h %0 .t\CS.seek 5ml C'g'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'N'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C'9'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&f& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&p/ +150 h / .t\CC.seek :open C'e( +416 h ( M&K& h &ӷ M&Q% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &|(- +264 h (- M&@& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:27:45CEST18-Oct-18\\n{1 => 0, 2 => 3, 3 => 19, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:27:39CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&;% h %\  A(V% h %X A(U. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +6 F*k% h %&K ."readyMFB"\ATV.dial :waste2 F*u % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *Z' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (W& h & M&Y& h &- M&x% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &o( +166 h ( M&q& h & A(i& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &[' +90 h ' A(C& h & .t\SE.seek 17.71 Pucks below Top Plate A('* +86 h '* A(I& h & M&=& h & .t\Elbow.seek 3 M&d( +7 h ( M&& h & A(h& h & .t\Forearm.seek 3 + 300 counts M&{'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down M&P& A( 'X h & M&~% h %B .t\Forearm.seek :retract M&y'\ +86 h 'X ."downElevator"\SE.coast A(5 +5 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&b1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 300ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)}+ +7 h + S)r% h % C'?& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X9 h 9Hd +100 C'9 h 9Ld +100 C'!9 h 9Pd +100 C'9 h 9Td +100 C'9 h 9Xd +100 C'29 h 9(\\d +100 C'F9 h 9@`d +100 C'<9 h 9Wdd +100 C'n9 h 9nhd +100 C'9 h 9ld +100 C'79 h 9pd +100 C'9 h 9td +100 C'(9 h 9xd +100 C' 9 h 9|d +100 C'E9 h 9d +100 C'A9 h 9d +100 C')9 h 9'd +100 C'99 h 9>d +100 C'O9 h 9Td +100 C'+9 h 9kd +100 C'69 h 9d +100 C'G9 h 9d +100 C'D9 h 9d +100 C'J9 h 9d +100 C'a9 h 9d +100 C'S9 h 9d +100 C',9 h 9 d +100 C'H9 h 9 d +100 C'39 h 96d +100 C'%9 h 9Ld +100 C'\\9 h 9bd +100 C'T9 h 9xd +100 C'R9 h 9d +100 C'\ 9 h 9d +100 C'9 h 9d +100 C'B9 h 9d +100 C'"9 h 9d +100 C'#9 h 9d +100 C' 9 h 9d +100 C'L9 h 9(d +100 C'f9 h 9>d +100 C'g9 h 9Td +100 C'l9 h 9id +100 C'9 h 9d +100 C'&9 h 9d +100 C'N9 h 9d +100 C'p9 h 9d +100 C'.9 h 9d +100 C'9 h 9d +100 C'Q9 h 9  d +100 C'_9 h 9 d +100 C'09 h 9 *d +100 C'@9 h 9 ?d +100 C'`9 h 9 Td +100 C'9 h 9 j d +100 C'K9 h 9 $d +100 C'^9 h 9 (d +100 C'j9 h 9 ,d +100 C'9 h 9 0d +100 C';9 h 9 4d +100 C'V9 h 9 8d +100 C'U9 h 9 % h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (!( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &ӷ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &F( +142 h ( M&<& h & A(n& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(7& h &  .t\SE.seek 1.71 Pucks below Top Plate A(': +244 h ': M&(& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &E' +25 h '  .t\Elbow.hold M&A, +7 h , .t\Elbow.reconfigure TightElbow A()- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(9' +27 h ' M&O% h %  A(+& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&6'8 +21 h '8 M&G& h & A(D& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &J' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(a' +36 h ' M&S& h & M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +90 h '\  .t\Elbow.seek CC M&3(ӷ +142 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&R/ +150 h / .t\CC.seek :open C'\ ( +416 h ( A(& h &  A(B& h &  M&"& h &ӷ M&#% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &L( +142 h ( M&f& h & A(g& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &l' +93 h '  .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &&1 +150 h 1 M&N% h %  A(p& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&.'8 +21 h '8 M&& h & A(Q& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &_' +22 h ' A(0% h % ."downElevator"\SE.seek :down A(@'X +100 (`% h %1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'^ @ +2 A(6 +17 h @ .t\CS.seek 10ml C'j' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C'; { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'V& h & .tHand.open M&U1 +2 h 1 M&k& h & M&u% h %\  .t\Elbow.seek FlushPuck::Garage M&-(- +165 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&q& h &- M&i% h %η .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &w(ӷ +265 h (ӷ M&|& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'4(W +416 h (W C'I& h &W .tHand.open M&P1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%h +150 h P%~% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%o' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%[  +37 h  .t\PS.seek 0ml P%'' +1598 h ' .t\PSV.dial PSR P%z +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%{  C'  h +75 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%y  h .t\PS.seek 0ml P%d' +2610 h ' .t\PSV.dial :air P%b  +38 h  .t\PS.seek 2.5ml P%e' +2609 h ' .t\PSV.dial :mixloop P%r +38 h .t\PS.seek 0ml P%?' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%m +38 h .t\PS.seek 0ml P%}' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&X& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&Z/ +150 h / .t\CC.seek :open C']( +416 h ( M&*& h &ӷ M&M% h % .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&t& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &5'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'/& h & M&:% h %\  A(% h %X A(>. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &- M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &<( +166 h ( M&n& h & A(7& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h & .t\SE.seek 16.71 Pucks below Top Plate A(('; +95 h '; A( & h & M&)& h & .t\Elbow.seek 3 M&O( +7 h ( M&+& h & A(G& h & .t\Forearm.seek 3 + 300 counts M&D'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &S/ +150 h / ."downElevator"\SE.seek :down M&& h & A(,'X M&F% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&E(ӷ +5 h 'X ."downElevator"\SE.coast A(5 +138 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +90 h ' .t\CC.seek :closed C'9(W +416 h (W .tHand.open M&J1 +150 h 1 M&A% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'H  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C''p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'B' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'"'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%#  +38 h  .t\PS.seek 2.5ml P%f' +2610 h ' .t\PSV.dial :mixloop P%g +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%T  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%3 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%&  +150 h  .t\PS.seek :empty P%N' +2610 h ' .t\CBV.dial :waste1 C'p @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'Q @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'0  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C'L @ +38 h @ .t\CS.seek 10ml C'`' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C'^'4 +2647 h '4 .t\CBV.dial :waste1 C'l @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C';& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&V% h %\  A(U% h %X A(k. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (u( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (.& h &  M&a& h &ӷ M&_% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +142 h ( M&x& h & A(!& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &@' +90 h ' A(& h &  .t\SE.seek 2.71 Pucks below Top Plate A(') +234 h ') M&& h & A(q& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &i' +26 h '  .t\Elbow.hold M&K, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(- h -+) .t\SE.seek 3.04 Pucks below Top Plate A(|' +27 h ' M&=% h %  A(-& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&C'8 +20 h '8 M&\ & h & A(I& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &P' +22 h ' .t\SE.seek 4.04 Pucks below Top Plate A(W' +37 h ' M&h& h & M&~% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&[(ӷ +142 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&{/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h &  A(y& h &  M&& h &ӷ M&b% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &r( +143 h ( M&e& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &?' +92 h '  .t\Elbow.hold M&m, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&X% h %  A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'8 +21 h '8 M&Z& h & A(w& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(4% h % ."downElevator"\SE.seek :down A(*'X +100 (M% h %  M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%v  + A(6 +112 h  P%8% h % .t\PS.seek 0.499ml P%' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%1  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%/  +37 h .t\CBV.dial AIV C':  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%>  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +250 h ' .t\PRV.dial :waste2 P%2 @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %5' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&71 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.seek :garage M&G'η +91 h 'η .tHand.close M&s/ +150 h / M&S& h &- M&% h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &<(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'((W +416 h (W C'D& h &W .tHand.open M&O1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C']& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%J @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%A  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%H  +75 h  .tdelay 2 seconds +200 P% % h % .t\PS.seek 1.499ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%j  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%B +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%#  +38 h  .tdelay 2 seconds +200 P%}% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %g'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%T  +75 h .tdelay 2 seconds +200 P%3% h %r .t\PS.seek 0.475ml P%&' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%f  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%p'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%Q  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %0' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck P%L @ +76 h @ .t\PS.seek 0ml P%"' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%`' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%l +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%;  +76 h  .t\PS.seek 2.5ml P%V' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%U +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%k  +75 h P%.% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %a' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'_ & +50 h & .t\AWV.select :waste C' +7 P%x% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %!'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %E' +1313 h ' .t\PSV.dial :air P%R  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%q +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '=& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'-& h &W ."MFBPCR"\ASV.dial :SPEdelivery F*\ @@T .t\AWV.select :air F*I  .t\AIV.dial :bypass .\PSV.dial PSR P%h +22 h  ."MFBPCR"delay 20 seconds +91 h P%~% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%@' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*Y I +128 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *i'6 +66 .t\PSV.dial :waste1 P%o  +38 h  .t\PS.seek 0ml P%z' +1597 h ' .t\PSV.dial PSR P%C +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%|' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%P +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*{  +22 h ' .t\PSV.dial PSR P% +21 h  F*9% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*b % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *e'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%'' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*[  +22 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *X' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%Z' +217 ."MFBPCR"\ATV.dial :AIV F*w I +43 h I F*4% h % ."MFBPCR"\AS.seek 0.469ml F*d' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%r +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P% +113 h P%?% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%W  +37 h  .t\PS.seek 0ml P%v' +740 h ' ."MFBPCR"delay 5 seconds +500 F*89 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F**% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%1 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%/' +2610 h ' .t\PSV.dial PSR P%: +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%2 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%7 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %)' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*+ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *m'V +850 h ' .t\PSV.dial :air P%s  +113 h  .t\PS.seek 2.5ml P%S' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %t' +5107 h ' .t\PSV.dial :air P%G  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*%  +43 h  F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *F'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*D% h %Q ."MFBPCR"\AS.seek 0.01ml F*O' +171 h ' .t\PSV.dial :mixloop P%, +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %]' +618 h ' ."MFBPCR"\ATV.dial :waste F*J n +129 h n ."MFBPCR"\AS.seek :empty F*\\'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*A  +85 h  F*H% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b * ' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*5  +43 h F*6% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *j'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'B% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'#'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'}' +1626 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'T  +37 h  +113  .t\CBV.dial :waste1 C'3 @ +38 h @ .t\CS.seek 0ml C'g'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*f  +21 h ."MFBPCR"delay 3 seconds +300 F*&% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'Q  +38 h  C'p% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*L  +21 h F*0% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*" n +108 h n F*l% h %& ."MFBPCR"\AS.seek 0.092ml F*`'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C';' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'U'4 +2648 h '4 C'k& h &W #tGaraging FlushPuck in Collection Clamp M&.& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +6 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ .t\Forearm.seek CC M&a' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'!( +416 h ( M&R& h &ӷ M&E% h % .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&=& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &-& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &I'η +91 h 'η .tHand.open M&h1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'Y& h & M&% h %\  A(o% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (@( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (C& h & M&z& h &- M&P% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &|( +165 h ( M&{& h & A(9& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(y& h & ."WCR"\SE.seek 15.71 Pucks below Top Plate A(i'L +106 h 'L A(K& h & M&b& h & ."WCR"\Elbow.seek 3 M& ( +7 h ( M&& h & A([& h & ."WCR"\Forearm.seek 3 + 300 counts M& 'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +148 h '&0 ."MFBPCR"delay 3 seconds +2 h / ."downElevator"\SE.seek :down A(e'X M&& h & M&w% h %B ."WCR"\Forearm.seek :retract M&4'\ +91 h '\  ."WCR"\Elbow.seek CC M&'(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&X& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Z' +64 ."MFBPCR"\ATV.dial :waste2 F* % +27 h ' ."WCR"\CC.seek :closed C'?(W +16 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +399 h (W ."WCR"Hand.open M&W1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &8'\ +11 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b **' +59 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)d+ +7 h + S)N% h % C'v& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'1  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h ."WCR"\SS.seek 0ml S)2' +216 h ' ."WCR"\SSV.dial CBV S)  +150 h  ."WCR"\CTV.dial :intake C'u  +75 h  ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +207 C'7% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F* @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*u I +86 h I ."MFBPCR"\AS.seek 0ml F*7'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*2'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *+'\  +1031 h '\  F*n% h %\  ."MFBPCR"\AS.seek 0.5ml F*s'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*S'\  +1031 h '\  F*G% h %\  ."MFBPCR"\AS.seek 0.5ml F*t'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*%% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *)' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1915h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*D  +64 h  ."MFBPCR"\ARV.dial :carrier F*,  +11 h  F*F% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F** n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*5  +22 h ."MFBPCR"delay 2 seconds +200 F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*# n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*^' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +108 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*p'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.65ml F*"' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*l  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*;'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226154 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'_  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'k @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'< @ +75 h @ +50 S)x% h % ."WCR"\CBV.dial between CSV and waste2b C'(  +94 h  C'R% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'a' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'=  'E  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'V'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'h  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '-' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'q  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'~  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'Y @ +75 h @ +50 S)o% h % ."WCR"\CBV.dial between CSV and waste2b C'P  +94 h  C'I% h % ."WCR"\CSV.dial between air and CTV C'z ` +57 h ` ."WCR"\CS.seek 7.288ml C'9' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'{  'y  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'b  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'[  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'| @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C'r% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'X' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'e'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'i  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '!' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'`  +151 h C'4& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&% h %\  A(c% h %X A(W. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (v( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (1& h &  M&N& h &ӷ M&M% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &@( +143 h ( M&Z& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &:' +89 h ' A(d& h &  ."WCR"\SE.seek 3.71 Pucks below Top Plate A(' +224 h ' M&/& h & A(>& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &u' +25 h '  ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(7- h -+ M&$&.,(\ #b ."WCR"\SE.seek 4.04 Pucks below Top Plate A(' +26 h ' M&% h %  A(2& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&n'8 +21 h '8 M&+& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &s' +22 h ' ."WCR"\SE.seek 5.04 Pucks below Top Plate A(G' +36 h ' M&S& h & M&%% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&)& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&D' +91 h ' ."WCR"Hand.close M&,/ +150 h / ."WCR"\CC.seek :open C'F( +416 h ( A(m& h &  A( & h &  M&*& h &ӷ M&J% h % ."WCR"\Forearm.seek :retract M&A'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &O( +142 h ( M&5& h & A(6& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &H' +93 h '  ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &B1 +150 h 1 M&\\% h %  A(#& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&& h & A(3& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +23 h ' A(^% h % ."downElevator"\SE.seek :down A(&'X +100 (Q% h % M&T% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +89 h '\  +22 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&p1 + A(6 + h 1 M&0& h & M&L% h %\  .t\Elbow.seek FlushPuck::Garage M&j(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&l'η +91 h 'η .tHand.close M&/ +150 h / M&"& h &- M& % h %η .t\Forearm.seek :retract M&.'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +264 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'(& h &W .tHand.open M&R1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'=& h &W C'E% h % .t\CSV.dial CSR C'a  +38 h  C'h% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'V'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '~'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '-'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'Y% h %4 .t\CSV.dial :flush C'o  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'P'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'I @ +37 h @ C'z% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  '{  +38 h  +113  .t\CBV.dial :waste1 C'9 @ +75 h @ .t\CS.seek 0ml C'b'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'[% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'w  +75 h  .t\CS.seek 0ml C'r'4 +766 h '4 #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +37 h @ C'i% h %p .t\CS.seek 7ml C'e'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'` '4  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'c'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C'1 +38 h .t\CS.seek 0ml C'N'4 +506 h '4 C'M& h &W C'Z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'd'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +37 h @ C'8% h %0 .t\CS.seek 5ml C'/'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'>'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'7'0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'2'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'+& h &W C'% h %4 .t\CSV.dial CSR C'S  +151 h  C'%% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C':'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'u  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C')' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  't  +38 h  +113  .t\CBV.dial :waste1 C'm @ +37 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'*  +38 h  C'J% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '5'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'B'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'\\  +37 h  C'#% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'^ 'A  +37 h +76 .t\CTV.dial :air C'O  +150 h .t\CS.seek 0ml C'Q'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'T'0 +506 h '0 .t\CSV.dial :waste C'g +38 h .t\CS.seek 0ml C'p'4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'L% h %4 .t\CSV.dial :flush C'H  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'G @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} +113 h .t\CTV.dial :air .\CS.seek 0ml C'&'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'v'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'"& h &W C' % h %4 .t\CSV.dial :flush C'f  +37 h  .t\CS.seek 3ml C'l'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'U% h %0 .t\CS.seek 5ml C'j'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +506 h '0 .t\CSV.dial :waste C'( +76 h .t\CS.seek 0ml C'R'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'=% h %4 .t\CSV.dial :flush C'E  +38 h  .t\CS.seek 5ml C'a'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C'<% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CTV C'q  .t\CTV.dial CSV C'V  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'~'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'Y% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C'-'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'I @ +37 h @ C'z% h %p .t\CS.seek 7ml C'P'i +416 h 'i ."connect"\CSV.dial CTV C'y  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'{  +75 h  .t\CS.seek 0ml C'9'4 +766 h '4 #tDisinfecting external Intake with kill C'C& h &W C'K% h %4 .t\CSV.dial CSR C'[  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'b'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'w' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'|  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ' '4 +607 h '4 .t\CSV.dial CSR C'  +113 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'r'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'X' +1372 h ' .t\CSV.dial CBV C'i +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&4& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &`(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &ӷ .t\Forearm.seek CC M&c' +91 h ' .tHand.close M&1/ +150 h / .t\CC.seek :open C'W( +416 h ( M&M& h &ӷ M&Z% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &N(- +264 h (- M&'& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &d'η +91 h 'η .tHand.open M&/1 +150 h 1 M&>% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'7& h & .tHand.open M&+1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &S(- +6 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &- .t\Forearm.seek :garage M&:'η +91 h 'η .tHand.close M&/ +150 h / M&u& h &- M&@% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &n' +91 h ' .t\CC.seek :closed C'(W +416 h (W C't& h &W .tHand.open M&?1 +150 h 1 M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +90 h '\  #t\Flushing the Collection Valve::Manifold C'*& h &W C'J% h %4 .t\CSV.dial CSR C'  +113 h  C'5% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'D'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '6'[ +463 h '[ .t\CSV.dial :waste C'\\ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +2009 h '4 #t\Purging external Intake C'B& h &W C'% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '^' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +75 h .t\CTV.dial :intake C'3  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'Q'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'g  +75 h #t\Flushing upper manifold C'T& h &W C'0% h %4 .t\CSV.dial :flush C'L  +75 h  .t\CS.seek 5ml C'H'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +37 h @ C'G% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'}'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'& +37 h .t\CS.seek 0ml C' '4 +507 h '4 C'v& h &W C'"% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'f'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'k% h %0 .t\CS.seek 5ml C'U'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'l'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +506 h '0 .t\CSV.dial :waste C'. +76 h .t\CS.seek 0ml C'('4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &ӷ .t\Forearm.seek CC M&R' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'<( +416 h ( M&a& h &ӷ M&q% h % .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&!& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Y'η +91 h 'η .tHand.open M&o1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:15 #\Preparing to sleep until Sun Oct 21 14:00:15 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00CEST21-Oct-18 #\Continuing at 14:00CEST21-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:127\\n{1 => 0, 2 => 6, 3 => 16, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:29:25CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST21-Oct-18" +279981 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct1915h300ml.pcr F*z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*{ % +87 h % F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *C'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*[% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*|  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*w  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +31 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*_% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % ."MFBPCR"delay 3 seconds +300 F*'% h %> ."MFBPCR"\AS.seek 0.835ml F*8'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +21 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1918h300ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*s% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*W n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *t'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F**  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*5  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*n% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*6 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*A  +107 h  ."MFBPCR"delay 7 seconds +700 F*#% h %< ."MFBPCR"\AS.seek 0.594ml F*O'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*3 n +21 h n ."MFBPCR"delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.65ml F*L' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*H'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1918h300ml.pcr F*}% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*v % +86 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*l'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*F'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*E  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*o% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +21 h F*Y% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*P% h %m ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1920h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*r'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*_  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*M I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*' % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.53ml F*`' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*/  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +22 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*+'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*2'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*N  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*:% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct1920h300ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*S @@T ."MFBPCR"\ASV.dial :SPEdelivery F*W  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*t  +151 h F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b **' +423 h ' ."MFBPCR"\ATV.dial :ASV F*J n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*?  +43 h  F*5% h % ."MFBPCR"\AS.seek 0.62ml F*n'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *D'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*6 % +43 h % F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\\' +423 h ' ."MFBPCR"\ATV.dial :air3 F*A% h % ."MFBPCR"\AS.seek 0.53ml F*#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*3% h % ."MFBPCR"\AS.seek 0.71ml F*^' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h % H ."MFBPCR"\AS.seek 0.735ml F*0' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*Q% h % Ű ."MFBPCR"\AS.seek 0.72ml F*p' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*G n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*L% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*} % +43 h % F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*"% h % ."MFBPCR"\AS.seek 0.579ml F*&' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*U% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :wasteR F*j  +22 h  ."MFBPCR"\AS.seek 0ml F*l'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *E'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*h % +108 h % F*F% h %V ."MFBPCR"\AS.seek 0.01ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*a' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*o% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*!' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*V n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Y  +21 h  ."MFBPCR"\AS.seek 0ml F*~'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *{' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*P n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *y'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +202 h 'R ."MFBPCR"\SPE.coast F*[  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*9 n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*b  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*w  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *r'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*X n +65 h n F*i% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *4' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*1 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *_'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*e  +43 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*x  +64 h  F*'% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *Z'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*c n +65 h n F*/% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *8' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*` I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*7  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *%'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*+ n +151 h n ."MFBPCR"\AS.seek 0ml F*d'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*2 % +43 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*u% h % ."MFBPCR"\AS.seek 0.96ml F*s'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +22 h F*m% h % ."MFBPCR"\AS.seek 0.97ml F*'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*J I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *5'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*D  +43 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*A I +43 h I ."MFBPCR"\AS.seek 0.02ml F*\\' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*3'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*#% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*^ % +43 h % F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*v  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*j  +32 h  ."MFBPCR"\ATV.dial :air3 F*k % +86 h % F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +21 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*E'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *y'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*- % +108 h % ."MFBPCR"\AS.seek 0ml F*9'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast + P%5 C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540123215.44 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )b h  C' ' h  P% %C h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)w% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X'4 S)' +27 h *k .t\Elbow.reconfigure LooseElbow .\Elbow.home.seek :clear M&$ &.,(#b &(d +47 h (d +59 / A(+& h &,K .t\SE.reconfigure SEconfig .\SE.home.seek :down A(#K-0-\ b (%'X +148 h 'X .t\SC.reconfigure SCsloppy .\SC.home.seek :initial A($-.(\ 1@~b (( +346 h ' +13 (  .t\Forearm.seek :retract M&2'\ +6 h '\  .t\Elbow.seek :hammer M&@(M + h '4 +92 (M .t\Forearm.reconfigure TightForearm .\Forearm.seek 5 + 350 counts M&#Q@2^d^\ b &d' +91 h ' M&:% h % .t\Forearm.seek :retract M&u'\ +91 h '\  M&N% h %\  P%& C'W& h &2 &3 ."openPC"\PC.reconfigure PCconfig .t\CC.reconfigure CCconfig ."openPC"\PC.home.seek :open P%$x2 ^22b .t\CC.home.seek :open P%s( C'$x2 ^22b 't( +222 h ( +3 ( +160 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 14:00:30 began in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:127\\n{1 => 0, 2 => 6, 3 => 16, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:30:06CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #3) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'm& h & .tHand.open M&1 +150 h 1 M&J& h &M M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &?(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b & 'η +91 h 'η .tHand.close M&n/ +150 h / M&D& h &- M&6% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +265 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'#(W +416 h (W C'\\& h &W .tHand.open M&31 +150 h 1 M&T% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'g& h &W C'O% h %4 .t\CSV.dial CSR C'0  +151 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'Q'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'p% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'L +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '}'4 +2010 h '4 #t\Purging external Intake C'H& h &W C'% h %4 .t\CSV.dial :flush C'v  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '"' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  h +76 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C''4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'&  +75 h #t\Flushing upper manifold C'f& h &W C'U% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'j'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'l% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h +113 h .t\CTV.dial :air .\CS.seek 0ml C'F'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +506 h '0 .t\CSV.dial :waste C', +38 h .t\CS.seek 0ml C''4 +506 h '4 C'E& h &W C'q% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 3ml C')'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'a% h %0 .t\CS.seek 5ml C'o'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'Y +75 h .t\CS.seek 0ml C'!'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&~& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &z(ӷ +6 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &ӷ .t\Forearm.seek CC M&P' +91 h ' .tHand.close M&{/ +150 h / .t\CC.seek :open C'[( +416 h ( M&K& h &ӷ M&-% h % .t\Forearm.seek :retract M&y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &$(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&91 +150 h 1 M&]% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +90 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:30:12CEST18-Oct-18\\n{1 => 0, 2 => 6, 3 => 16, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:30:06CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*I% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'S& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&b% h %\  A(% h %X A(C. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +7 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *w' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&|& h &- M& % h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +166 h ( M& & h & A(X& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(r& h & .t\SE.seek 14.71 Pucks below Top Plate A(1'] +116 h '] A(4& h & M&_& h & .t\Elbow.seek 3 M&e( +7 h ( M&M& h & A('& h & .t\Forearm.seek 3 + 300 counts M&x'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down M&/& A('X h & M&Z% h %B .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.seek CC M&+(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &N' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&W1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 300ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)2+ +7 h + S)d% h % C'm& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +5064 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*p  h  ."readyMFB"\ASV.dial :waste1 F*Q  h +1311 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'L% h %4 S)% h % S)H% h % .tIntake.select :close S)0 +6 )% h % .t\SSV.dial :exhaust S)v  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'U9 h 9Td +100 C' 9 h 9kd +100 C' 9 h 9d +100 C'9 h 9d +100 C'l9 h 9d +100 C'j9 h 9d +100 C'h9 h 9d +100 C'.9 h 9d +100 C'F9 h 9 d +100 C'}9 h 9 d +100 C'f9 h 96d +100 C'=9 h 9Ld +100 C'E9 h 9bd +100 C'q9 h 9xd +100 C'k9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'a9 h 9d +100 C'R9 h 9d +100 C')9 h 9d +100 C'9 h 9d +100 C'Y9 h 9(d +100 C'~9 h 9>d +100 C'o9 h 9Td +100 C';9 h 9id +100 C',9 h 9d +100 C'\ 9 h 9d +100 C'V9 h 9d +100 C'{9 h 9d +100 C'^9 h 9d +100 C'#9 h 9d +100 C'-9 h 9  d +100 C'9 h 9 d +100 C'B9 h 9 *d +100 C'P9 h 9 ?d +100 C'(9 h 9 Td +100 C'99 h 9 j d +100 C'K9 h 9 $d +100 C'z9 h 9 (d +100 C'y9 h 9 ,d +100 C'I9 h 9 0d +100 C'b9 h 9 4d +100 C'9 h 9 8d +100 C'C9 h 9 . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (P( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (r& h & M&:& h &- M&/% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &$( +165 h ( M&x& h & A(!& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &M' +90 h ' A(8& h & ."WCR"\SE.seek 12.71 Pucks below Top Plate A(X' +136 h ' A(K& h & M&W& h & ."WCR"\Elbow.seek 3 M&e( +7 h ( M&& h & A(i& h & ."WCR"\Forearm.seek 3 + 300 counts M&'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &7/ +118 h '&0 ."MFBPCR"delay 3 seconds +32 h / ."downElevator"\SE.seek :down M&[& A(5'X h & M&% h %B ."WCR"\Forearm.seek :retract M&<'\ +91 h '\  ."WCR"\Elbow.seek CC M&%(ӷ +45 h 'X ."downElevator"\SE.coast A(5 +97 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +35 ."MFBPCR"\ATV.dial :waste2 F*w % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +13 h ' ."WCR"\CC.seek :closed C'u(W +416 h (W ."WCR"Hand.open M&1 +131 h 'R ."MFBPCR"\ATV.dial :air2 F**  +19 h 1 M&N% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +2 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *6' +89 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)V% h % C'?& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'n  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +43 h % F*r% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*4% h % ."MFBPCR"\AS.seek 0.579ml F*x' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*W% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*i n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*7'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*$ % +107 h % F*M% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*d n +107 h n ."MFBPCR"\ASV.dial :wasteR F*X% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*e I +86 h I F*5% h % ."MFBPCR"\AS.seek 0.12ml F*<' +625 h ' ."MFBPCR"\ATV.dial :air3 F*( % +21 h % F*3% h % ."MFBPCR"\AS.seek 0.13ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +21 h F**% h % ."MFBPCR"\AS.seek 0.23ml F*V' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h % ."MFBPCR"\AS.seek 0.255ml F*'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*P% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*O n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1520 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *0'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*; I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*p'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*D  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b * '\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*@% h %(A ."MFBPCR"\AS.seek 0.6ml F*.'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*=% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *"' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +3472 h 'V ."MFBPCR"\SPE.coast F*1  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2115h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*G  +64 h  ."MFBPCR"\ARV.dial :carrier F*H  +11 h  F*Q% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*A  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*) n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*f  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*2% h % ."MFBPCR"\AS.seek 0.536ml F*q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +108 h  ."MFBPCR"delay 7 seconds +700 F*-% h %< ."MFBPCR"\AS.seek 0.594ml F*{'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +21 h n ."MFBPCR"delay 2 seconds +200 F*o% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*B  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Y% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*#  +43 h  ."MFBPCR"\ASV.dial :waste1 F*9  +64 h ."MFBPCR"\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*^ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226183 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'L @ +76 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'l  +94 h  C'S% h % ."WCR"\CSV.dial between air and CTV C'v ` +19 h ` ."WCR"\CS.seek 7.288ml C'h' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'_  '  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'C'4 +1920 h '4 ."WCR"\CBV.dial :waste2b C''  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ']' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'k  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Z  +38 h  C',% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'>' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'| @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'/  +94 h  C'4% h % ."WCR"\CSV.dial between air and CTV C': ` +57 h ` ."WCR"\CS.seek 7.288ml C'!' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'8  'x  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'W  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'i  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'7' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'$ @ +75 h @ +50 S)M% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'd% h % ."WCR"\CSV.dial between air and CTV C'X ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'w  'e  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '5'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '(' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'3  +150 h C'<& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&N% h %\  A(*% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (?( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (n& h &  M&V& h &ӷ M&m% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &P( +142 h ( M&& h & A(O& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &g' +90 h ' A(& h &  ."WCR"\SE.seek 6.71 Pucks below Top Plate A(' +195 h ' M&T& h & A(0& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &;' +26 h '  ."WCR"\Elbow.hold M&t, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- h -+ M&$&.,(\ #b ."WCR"\SE.seek 7.04 Pucks below Top Plate A(D' +27 h ' M&% h %  A(p& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M& & h & A(@& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 8.04 Pucks below Top Plate A('w +37 h 'w M&.& h & M&=% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&F(ӷ +143 h (ӷ M&"& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ ."WCR"\Forearm.seek CC M&G' +90 h ' ."WCR"Hand.close M&H/ +150 h / ."WCR"\CC.seek :open C'Q( +416 h ( A(J& h &  A(& h &  M&6& h &ӷ M&j% h % ."WCR"\Forearm.seek :retract M&&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &U( +143 h ( M&A& h & A( & h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &}' +92 h '  ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &a1 +150 h 1 M&E% h %  A()& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&'8 +21 h '8 M&\\& h & A(f& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &2' +22 h ' A(z% h %w ."downElevator"\SE.seek :down A(\ 'X +100 (-% h % M&q% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +82 h 'X ."downElevator"\SE.coast A(5 +8 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'o& h & .tHand.open M&{1 +2 h 1 M&B& h & M&Y% h %\  .t\Elbow.seek FlushPuck::Garage M&y(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &- .t\Forearm.seek :garage M&I'η +91 h 'η .tHand.close M&b/ +150 h / M&R& h &- M&% h %η .t\Forearm.seek :retract M&^'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &L' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'l& h &W .tHand.open M&S1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'_& h &W C'% h % .t\CSV.dial CSR C'h  +37 h  C''% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'C'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'k% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Z'[ +463 h '[ .t\CSV.dial :waste C', +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ']'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'>& h &W C'|% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '/'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C':% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV C'8  .t\CTV.dial CSV C'x  +37 h  +113  .t\CBV.dial :waste1 C'! @ +76 h @ .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'i% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'7% h %p .t\CS.seek 7ml C'$'i +416 h 'i ."connect"\CSV.dial CTV C'M  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'd'4 +766 h '4 #t\Flushing upper manifold C'X& h &W C'[% h %4 .t\CSV.dial :flush C'e  +75 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'5'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 '<  +38 h +75 .t\CTV.dial :air C'N  +150 h .t\CS.seek 0ml C'*'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'n +38 h .t\CS.seek 0ml C'V'4 +506 h '4 C'm& h &W C'% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +38 h @ C'T% h %0 .t\CS.seek 5ml C'0'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'p'4 +507 h '4 #tDisinfecting external Intake with mfbkill C' & h &W C'@% h %4 .t\CSV.dial CSR C'.  +150 h  C'=% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'g'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'P' +416 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :intake C';  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'"' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'1  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'D  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C't'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'H' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'u  +37 h  +113  .t\CBV.dial :waste1 C'J @ +38 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'6  +37 h  C'j% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'A'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +37 h  .t\CBV.dial :waste2b C'F  +76 h  .t\CS.seek 0ml C'a'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'E  +38 h  C')% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\\'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z '&  +38 h +75 .t\CTV.dial :air C'U  +151 h .t\CS.seek 0ml C'-'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +506 h '0 .t\CSV.dial :waste C'o +38 h .t\CS.seek 0ml C'{'4 +506 h '4 #t\Flushing upper manifold C'B& h &W C'Y% h %4 .t\CSV.dial :flush C'}  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'#% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'2 +113 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'b'4 +507 h '4 C'R& h &W C'% h %4 .t\CSV.dial :flush C'~  +37 h  .t\CS.seek 3ml C'I'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C'y'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'^'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'Q'0 +506 h '0 .t\CSV.dial :waste C'l +75 h .t\CS.seek 0ml C'S'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'v& h &W C'_% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +38 h @ C'L% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'k  'C  +37 h  +113  .t\CBV.dial :waste1 C', @ +38 h @ .t\CS.seek 0ml C'Z'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'>& h &W C'|% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C']'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C':% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CTV C'8  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'x  +75 h  .t\CS.seek 0ml C'!'4 +766 h '4 #tDisinfecting external Intake with kill C'r& h &W C'K% h %4 .t\CSV.dial CSR C'i  +37 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'W'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 '  +38 h +75 .t\CTV.dial :intake C'M  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'$  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +607 h '4 .t\CSV.dial CSR C'[  +113 h  C'e% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'w'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +6 h (ӷ M&N& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&*' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'( +416 h ( M&m& h &ӷ M&% h % .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +265 h (- M&%& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&01 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 +2 h 1 M&@& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &.(- +6 h (- M&=& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &- .t\Forearm.seek :garage M&g'η +91 h 'η .tHand.close M&/ +150 h / M&;& h &- M&P% h %η .t\Forearm.seek :retract M&1'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +264 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C't(W +416 h (W C'u& h &W .tHand.open M&1 +150 h 1 M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'6& h &W C'j% h %4 .t\CSV.dial CSR C'  +113 h  C'A% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'G'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'F% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'E +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ')'4 +2010 h '4 #t\Purging external Intake C'a& h &W C'\\% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'z' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'U +76 h .t\CTV.dial :intake C'f  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'-'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'o  +76 h #t\Flushing upper manifold C'q& h &W C'B% h %4 .t\CSV.dial :flush C'Y  +75 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'% h %p .t\CS.seek 7ml C'#'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'2'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'\ +38 h .t\CS.seek 0ml C''4 +507 h '4 C'?& h &W C'R% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'~'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'I'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'y'0 +506 h '0 .t\CSV.dial :waste C'^ +75 h .t\CS.seek 0ml C'l'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&Q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M&'/ +150 h / .t\CC.seek :open C'L( +415 h ( M&h& h &ӷ M&k% h % .t\Forearm.seek :retract M&C'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &,(- +265 h (- M&(& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &|'η +91 h 'η .tHand.open M&1 +150 h 1 M&Z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:30 #\Preparing to sleep until Tue Oct 23 14:00:30 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00CEST23-Oct-18 #\Continuing at 14:00CEST23-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:127\\n{1 => 0, 2 => 9, 3 => 13, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:31:53CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST23-Oct-18" +280002 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2115h300ml.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*8  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *r'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +21 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +31 h  ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*m% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*%% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*0% h %m ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2118h300ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ARV.dial :carrier F*D  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*.'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *u'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*J  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +22 h ."MFBPCR"delay 2 seconds +200 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*A  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*F  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*\\'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +107 h  ."MFBPCR"delay 7 seconds +700 F*)% h %< ."MFBPCR"\AS.seek 0.594ml F*U'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*Y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*-  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*}'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2118h300ml.pcr F*2% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*9  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*I'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*Q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*_  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*'% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *"'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*>  +32 h  ."MFBPCR"\ATV.dial :air3 F*v % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*: % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*8'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*/% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *['V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2120h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*X% h %V ."MFBPCR"\AS.seek 0.5ml F*d'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*n  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*m I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*% % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*3' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*0  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F* '\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*@ n +22 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*p'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*P  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*V  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*g% h % \\ ."MFBPCR"\AS.seek 0.326ml F*D'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2120h300ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*. @@T *  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*u  +151 h F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *6' +423 h ' ."MFBPCR"\ATV.dial :ASV F*j n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*A% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *F'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *G'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*w'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *E' +424 h ' ."MFBPCR"\ATV.dial :air3 F*&% h % ."MFBPCR"\AS.seek 0.53ml F*)' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*f% h % ."MFBPCR"\AS.seek 0.71ml F*z' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h % H ."MFBPCR"\AS.seek 0.735ml F*B' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*-% h % Ű ."MFBPCR"\AS.seek 0.72ml F*{' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Y% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*# n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*2 % +43 h % F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*R% h % ."MFBPCR"\AS.seek 0.579ml F*\ ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :wasteR F*y  +22 h  ."MFBPCR"\AS.seek 0ml F*I'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*' % +108 h % F*Q% h %V ."MFBPCR"\AS.seek 0.01ml F*"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*t% h % ."MFBPCR"\AS.seek 0.58ml F*h' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*S% h % q ."MFBPCR"\AS.seek 0.605ml F*>'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*(' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*C n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*|  +21 h  ."MFBPCR"\AS.seek 0ml F*Z'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *x' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*/ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *8'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *]'R +202 h 'R ."MFBPCR"\SPE.coast F*i  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*r'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*! n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*W  +42 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *7' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*M I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *e': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*X  +64 h  F*[% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *d'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*n I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*5  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*%% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F** n +65 h n F*0% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *T' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*3 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *O': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*@ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *='Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*p % +43 h % F*P% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*;% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %0 ."MFBPCR"\AS.seek 0.985ml F*D' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*J% h % ."MFBPCR"\AS.seek 0.97ml F*1'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*j I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *A'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *F'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*G  +43 h  F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *a' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *w' +9988 h ' ."MFBPCR"\ATV.dial AIV F*& I +43 h I ."MFBPCR"\AS.seek 0.02ml F*E' +9880 h ' ."MFBPCR"\ATV.dial :waste F*\\ n +129 h n ."MFBPCR"\AS.seek 0ml F*f'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*)% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*q  +21 h ."MFBPCR"\ATV.dial :air3 F*z % +43 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*Y'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*}% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*?  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*R  +64 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*'  +21 h F*Q% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*S% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*>' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *8'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*] % +108 h % ."MFBPCR"\AS.seek 0ml F*!'V +2547 h 'V ."shutdown"\AS.coast .\SS.coast .\CC.coast F*5 S)5 ."shutdown"\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540296030.89 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )W h  C' ' h  P% %r h  F* *M h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ S)&\ P% 0\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *7  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C' & h & S)#.0X 0, 2 => 9, 3 => 13, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:32:34CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #4) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'J& h & .tHand.open M&D1 +150 h 1 M&j& h &M M&1% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +166 h (- M&A& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &'η +91 h 'η .tHand.close M&/ +150 h / M&G& h &- M& % h %η .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &a(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C')(W +416 h (W C'E& h &W .tHand.open M&f1 +150 h 1 M&q% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &z'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'o& h &W C'U% h %4 .t\CSV.dial CSR C'B  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '-'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '{% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '#'[ +463 h '[ .t\CSV.dial :waste C'Y +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '2'4 +2009 h '4 #t\Purging external Intake C'}& h &W C'% h %4 .t\CSV.dial :flush C'?  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'R' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  '9 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'b'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'\  +75 h #t\Flushing upper manifold C'~& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'I% h %p .t\CS.seek 7ml C'^'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +113 h .t\CTV.dial :air .\CS.seek 0ml C'Q'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'H +37 h .t\CS.seek 0ml C't'4 +507 h '4 C'& h &W C'k% h %4 .t\CSV.dial :flush C'S  +37 h  .t\CS.seek 3ml C'"'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +38 h @ C'h% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'>'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'C'0 +506 h '0 .t\CSV.dial :waste C'| +76 h .t\CS.seek 0ml C'('4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&Z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&/' +91 h ' .tHand.close M&x/ +150 h / .t\CC.seek :open C'i( +416 h ( M&K& h &ӷ M&]% h % .t\Forearm.seek :retract M&8'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &s(- +264 h (- M&,& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &+'η +91 h 'η .tHand.open M&!1 +150 h 1 M&`% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:32:39CEST18-Oct-18\\n{1 => 0, 2 => 9, 3 => 13, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:32:34CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*4% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K C'.& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&W% ."readyMFB"\AFV.select :holdingCoil F* h %\  A(% h %X A(r. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (M( +6 F*7% h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ( & h & M&$& h &- M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &[( +166 h ( M&e& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(d& h & .t\SE.seek 11.71 Pucks below Top Plate A(n'Ɛ +145 h 'Ɛ A(<& h & M&& h & .t\Elbow.seek 3 M&5( +7 h ( M&m& h & A(%& h & .t\Forearm.seek 3 + 300 counts M& 'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &*/ +150 h / ."downElevator"\SE.seek :down M&N& A(0'X h & M&% h %B .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.seek CC M& (ӷ +55 h 'X ."downElevator"\SE.coast A(5 +87 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  .t\SC.coast #collectSample 300ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)p+ +7 h + S)% h % C'J& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  .t\CBV.dial SSV +150 h  .t\CTV.dial :air,avoiding: :intake C'|  +151 h ."CSpush"\CSV.dial CTV .t\SS.seek 10ml S)C' +100 ."CSpush"\CSV.dial :air C'Z @ +37 h @ ."CSpush"\CS.reconfigure CSfast .\CS.seek 0ml C'#d @b ''4 +7 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S) +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'9 h 9kd +100 C'9 h 9d +100 C'b9 h 9d +100 C'I9 h 9d +100 C'y9 h 9d +100 C''9 h 9d +100 C'^9 h 9d +100 C'Q9 h 9 d +100 C'29 h 9 d +100 C'~9 h 96d +100 C'_9 h 9Ld +100 C'9 h 9bd +100 C'k9 h 9xd +100 C'9 h 9d +100 C't9 h 9d +100 C'v9 h 9d +100 C'h9 h 9d +100 C'S9 h 9d +100 C'"9 h 9d +100 C'>9 h 9d +100 C'|9 h 9(d +100 C'Z9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C'H9 h 9d +100 C'9 h 9d +100 C'C9 h 9d +100 C'x9 h 9d +100 C'z9 h 9d +100 C')9 h 9d +100 C']9 h 9  d +100 C'/9 h 9 d +100 C'a9 h 9 *d +100 C',9 h 9 ?d +100 C'l9 h 9 Td +100 C'!9 h 9 j d +100 C'K9 h 9 $d +100 C':9 h 9 (d +100 C'89 h 9 ,d +100 C'49 h 9 0d +100 C'W9 h 9 4d +100 C'9 h 9 8d +100 C'r9 h 9 '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &|( +142 h ( M&Z& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &' +93 h '  .t\Elbow.hold M&H, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&C% h %  A(x& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&z'8 +20 h '8 M&)& h & A(]& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &/' +23 h ' A(a% h %f ."downElevator"\SE.seek :down A(,'X +100 (l% h % M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +72 h 'X ."downElevator"\SE.coast A(5 +17 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C': @ +19 h @ .t\CS.seek 10ml C'8' +6 h ' .t\CSV.dial between air and CTV C'4 ` +19 h ` .t\AIV.dial :bypass C'W { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&r1 +2 h 1 M&7& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&`(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &- .t\Forearm.seek :garage M&+'η +91 h 'η .tHand.close M&./ +150 h / M&e& h &- M&% h %η .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &m' +91 h ' .t\CC.seek :closed C'L(W +416 h (W C'<& h &W .tHand.open M&*1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%% +151 h P%N% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%[' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%g +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'n  h +75 .t\PSV.dial :mixloop P%5 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%T  h .t\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%i' +2610 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.seek 0ml P%J' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%M' +2610 h ' .t\PSV.dial :mixloop P%1 +37 h .t\PS.seek 0ml P%p' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&@& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +7 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&D/ +150 h / .t\CC.seek :open C'3( +416 h ( M& & h &ӷ M&;% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &G(- +265 h (- M&w& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &&'η +91 h 'η .tHand.open M&1 +150 h 1 M&j% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &F'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'q& h & M&o% h %\  A(U% h %X A(B. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (E( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (\\& h & M&P& h &- M&{% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Y( +166 h ( M&#& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(& h & .t\SE.seek 10.71 Pucks below Top Plate A(' +155 h ' A(O& h & M&R& h & .t\Elbow.seek 3 M&( +7 h ( M&& h & A(b& h & .t\Forearm.seek 3 + 300 counts M&I'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &^/ +150 h / ."downElevator"\SE.seek :down M&Q& A(}'X h & M&-% h %B .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.seek CC M&9(ӷ +64 h 'X ."downElevator"\SE.coast A(5 +79 h (ӷ M&_& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +90 h ' .t\CC.seek :closed C'((W +416 h (W .tHand.open M&y1 +150 h 1 M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'2  +151 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'v'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'h' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'S'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%Z' +2610 h ' .t\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%k  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%~ +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%C' +2610 h ' .t\CBV.dial :waste1 C'x @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C')  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'] @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'a  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'> ` +19 h ` .t\CSV.dial :air C'| @ +38 h @ .t\CS.seek 10ml C'l' +2647 h ' .t\CSV.dial CTV C'!  +38 h  .t\CS.seek 0ml C':'4 +2647 h '4 .t\CBV.dial :waste1 C'4 @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'W  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&r% h %\  A(7% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (z& h &  M&'& h &ӷ M&/% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &$( +142 h ( M&X& h & A(,& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &f' +90 h ' A(& h &  .t\SE.seek 8.71 Pucks below Top Plate A(H' +175 h ' M&.& h & A(e& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &' +25 h '  .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(+- h -+ M&$&.,(\ #b .t\SE.seek 9.04 Pucks below Top Plate A(s'f +27 h 'f M&% h %  A(`& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&d'8 +21 h '8 M&t& h & A(<& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &*' +22 h ' .t\SE.seek 10.04 Pucks below Top Plate A( 'U +36 h 'U M&%& h & M&N% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &g& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'n( +416 h ( A(5& h &  A(T& h &  M&& h &ӷ M&% h % .t\Forearm.seek :retract M&0'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&i& h & A( & h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &J' +93 h '  .t\Elbow.hold M&1, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &M1 +150 h 1 M&@% h %  A(A& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&6'8 +21 h '8 M&D& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &=' +22 h ' A(L% h %U ."downElevator"\SE.seek :down A( 'X +100 (;% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +61 h 'X ."downElevator"\SE.coast A(5 +28 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%  +113 h  P%V% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%j +38 h .t\PRV.dial :air P%  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%q  +38 h .t\CBV.dial AIV C'o  +37 h .t\PRV.dial between CBV and air P%U  +19 h  .t\PRV.dial :air P%B  +19 h  P%\\% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %P' +250 h ' .t\PRV.dial :waste2 P%{ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %&' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'#& h & .tHand.open M&1 +2 h 1 M&& h & M&G% h %\  .t\Elbow.seek FlushPuck::Garage M&O(- +165 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&b'η +91 h 'η .tHand.close M&F/ +150 h / M&^& h &- M&Q% h %η .t\Forearm.seek :retract M&-'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Y(ӷ +265 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'I(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +91 h '\  C'3& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%y @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%\  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%2  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.499ml P%' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%E' +1094 h ' .t\PSV.dial :air P%8  +37 h  .t\PS.seek 0ml P%v' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%h +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%"  +37 h  .tdelay 2 seconds +200 P%p% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%k  +75 h .tdelay 2 seconds +200 P%~% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%Z  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%x'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%)  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%]  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %a' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%> +75 h .t\PTV.dial :PRV,avoiding: :puck P%| @ +75 h @ .t\PS.seek 0ml P%S' +2610 h ' .t\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%l' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%4 +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%W' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%r' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%7 +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%z% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %'' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'/ & +50 h & .t\AWV.select :waste C' +6 P%X% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %,'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %9' +1313 h ' .t\PSV.dial :air P%.  +38 h  .t\PS.seek 2.5ml P%e' +2610 h ' .t\PSV.dial :PML P%+ +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'`& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*t @@T .t\AWV.select :air F*<  .t\AIV.dial :bypass .\PSV.dial PSR P%% +22 h  ."MFBPCR"delay 20 seconds +91 h P%N% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%f' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*$ I +129 h I F*H% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%[  +37 h  .t\PS.seek 0ml P%g' +1598 h ' .t\PSV.dial PSR P%d +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%s' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%* +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%m +21 h  F*(% h %6 ."MFBPCR"\AS.seek 0.329ml F*5'ͯ +92 h P%T% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *i'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*M% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *@' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%A +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%D' +217 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*L% h % ."MFBPCR"\AS.seek 0.469ml F*0' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%;' +2609 h ' .t\PSV.dial PSR P% +113 h P%J% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%6' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +739 h ' ."MFBPCR"delay 5 seconds +500 F*V9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*= n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F* % h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%C +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%j' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial PSR P%o +113 h P%U% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%B' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\\  +37 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%{ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%P' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%G  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %R' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *1'V +849 h ' .t\PSV.dial :air P%F  +113 h  .t\PS.seek 2.5ml P%^' +2610 h ' .t\PSV.dial :mixloop P%Q +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %w' +5107 h ' .t\PSV.dial :air P%b  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb % ' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*_  +43 h  F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *-'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*n I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P%} +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %3' +618 h ' ."MFBPCR"\ATV.dial :waste F*y n +129 h n ."MFBPCR"\AS.seek :empty F*'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*\  +86 h  F*2% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*&  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *8'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'E  +37 h  C'h% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'"'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'p' +1625 h ' ."connect"\CSV.dial CTV C'v  .t\CTV.dial CSV C'k  +38 h  +113  .t\CBV.dial :waste1 C'~ @ +37 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*Z  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*)'ݏ +110 h '4 .t\CSV.dial CSR C']  +37 h  C'x% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'>'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*|  +22 h F*a% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*!'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*S n +108 h n F*4% h %& ."MFBPCR"\AS.seek 0.092ml F*l'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'W  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'7'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +7 h (ӷ M&/& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.seek CC M&'' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C',( +416 h ( M&.& h &ӷ M&9% h % .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &e(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &<'η +91 h 'η .tHand.open M&%1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C'$& h & M&H% h %\  A([% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (f( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (d& h & M&g& h &- M&*% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &s( +166 h ( M& & h & A((& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &m' +89 h ' A(T& h & ."WCR"\SE.seek 9.71 Pucks below Top Plate A(' +166 h ' A(K& h & M&& h & ."WCR"\Elbow.seek 3 M&5( +7 h ( M& & h & A(& h & ."WCR"\Forearm.seek 3 + 300 counts M&M'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +88 h '&0 ."MFBPCR"delay 3 seconds +62 h / ."downElevator"\SE.seek :down M&i& h & A(A'X M&% h %B ."WCR"\Forearm.seek :retract M&L'\ +91 h '\  ."WCR"\Elbow.seek CC M&(ӷ +75 h 'X ."downElevator"\SE.coast A(5 +67 h (ӷ M&@& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &D' +5 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *J'R +43 h ' ."WCR"\CC.seek :closed C';(W +416 h (W ."WCR"Hand.open M& 1 +101 h 'R ."MFBPCR"\ATV.dial :air2 F*6  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *V' +27 h 1 M&=% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)0+ +7 h + S)C% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*W % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*z I +21 h I F*/% h %v ."MFBPCR"\AS.seek 0.059ml F*X'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*Y% h %H ."MFBPCR"\AS.seek 0.069ml F*?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*I  +22 h F*.% h % ."MFBPCR"\AS.seek 0.769ml F*9' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*`' k +526 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*%% h % k ."MFBPCR"\AS.seek 0.779ml F*N'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*+ I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *$' +4416 h ' ."MFBPCR"\ATV.dial :waste F*H n +129 h n ."MFBPCR"\AS.seek :empty F*['R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*c % +42 h % F*d% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *g' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F**  +21 h F*<% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %  ."MFBPCR"\AS.seek 0.604ml F*e'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*s % +108 h % F*m% h %V ."MFBPCR"\AS.seek 0.59ml F*@' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F*' +10076 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*5 I +86 h I F*A% h % ."MFBPCR"\AS.seek 0.12ml F*L' +626 h ' ."MFBPCR"\ATV.dial :air3 F*l % +21 h % F*,% h % ."MFBPCR"\AS.seek 0.13ml F*6' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*=% h % ."MFBPCR"\AS.seek 0.23ml F*C' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*J'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*f% h %X ."MFBPCR"\AS.seek 0.24ml F*0' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*U n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*o  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *B'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*j @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*{'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*G  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*#% h %\  ."MFBPCR"\AS.seek 0.5ml F*F'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*P% h %(A ."MFBPCR"\AS.seek 0.6ml F*^'\  +1032 h '\  F*b% h %\  ."MFBPCR"\AS.seek 0.5ml F*w'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*_% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *R' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*Q % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *1'V +3473 h 'V ."MFBPCR"\SPE.coast F*n  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2315h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*}  +10 h  F*-% h %V ."MFBPCR"\AS.seek 0.5ml F*O'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*&  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*" n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*v% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*~  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*k'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*]% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*8'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*a  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*|% h %\  ."MFBPCR"\AS.seek 0.65ml F*S' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*)  +43 h  ."MFBPCR"\ASV.dial :waste1 F*!  +65 h ."MFBPCR"\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226213 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'/  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'Y @ +76 h @ +50 S)X% h % ."WCR"\CBV.dial between CSV and waste2b C'I  +94 h  C'.% h % ."WCR"\CSV.dial between air and CTV C'? ` +19 h ` ."WCR"\CS.seek 7.288ml C''' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'9  '  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'r'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'%  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '`' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'+  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'N  +38 h  C'H% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'c' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'd' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'$ @ +75 h @ +50 S)[% h % ."WCR"\CBV.dial between CSV and waste2b C'*  +94 h  C'<% h % ."WCR"\CSV.dial between air and CTV C'g ` +57 h ` ."WCR"\CS.seek 7.288ml C'(' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'T  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'e' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C's @ +75 h @ +50 S)m% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'@' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'5  'M  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'A'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'l' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C',  +151 h C'L& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M& % h %\  A(=% h %X A(6. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&C& h &ӷ M&D% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &f( +142 h ( M&J& h & A(U& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &o' +90 h ' A(0& h &  ."WCR"\SE.seek 9.71 Pucks below Top Plate A(\\' +166 h ' M&q& h & A(B& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &' +25 h '  ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."WCR"\SE.seek 10.04 Pucks below Top Plate A(G'U +26 h 'U M&% h %  A({& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&#'8 +21 h '8 M&& h & A(P& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &F' +22 h ' ."WCR"\SE.seek 11.04 Pucks below Top Plate A(b'D +36 h 'D M&^& h & M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &w'\ +89 h '\  ."WCR"\Elbow.seek CC M&Q(ӷ +143 h (ӷ M&R& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&}/ +150 h / ."WCR"\CC.seek :open C'-( +416 h ( A(1& h &  A(O& h &  M&V& h &ӷ M&y% h % ."WCR"\Forearm.seek :retract M&\ '\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +142 h ( M&&& h & A(& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &2' +93 h '  ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &h1 +150 h 1 M&% h %  A("& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts +2 M&v'8 +20 h '8 M&E& h & A(~& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &p' +23 h ' A(:% h %D ."downElevator"\SE.seek :down A('X +100 (]% h % M&k% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +52 h 'X ."downElevator"\SE.coast A(5 +37 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&x1 +2 h 1 M&a& h & M&|% h %\  .t\Elbow.seek FlushPuck::Garage M&8(- +166 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.seek :garage M&4'η +91 h 'η .tHand.close M&W/ +150 h / M&S& h &- M&>% h %η .t\Forearm.seek :retract M&z'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &/(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'X(W +416 h (W C'I& h &W .tHand.open M&.1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'9% h % .t\CSV.dial CSR C''  +38 h  C'%% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'r'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '+% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'N'[ +462 h '[ .t\CSV.dial :waste C'H +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '`'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'c& h &W C'$% h %4 .t\CSV.dial :flush C'[  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '*'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'g% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'T  +38 h  +113  .t\CBV.dial :waste1 C'( @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'e @ +37 h @ C'% h %p .t\CS.seek 7ml C's'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'm  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing upper manifold C'& h &W C'i% h %4 .t\CSV.dial :flush C'M  +75 h  .t\CS.seek 5ml C'5'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +37 h @ C'% h %p .t\CS.seek 7ml C'A'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', 'L  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'='4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'6'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'C'4 +506 h '4 C'D& h &W C'J% h %4 .t\CSV.dial :flush C'U  +38 h  .t\CS.seek 3ml C'0'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'q% h %0 .t\CS.seek 5ml C'B'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'{'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'P% h %4 .t\CSV.dial CSR C'^  +151 h  C'_% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C'o'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'f' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'R' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'n  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +607 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'G  +113 h  C'F% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '}' +710 h ' ."connect"\CSV.dial CTV C'w  .t\CTV.dial CSV C';  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C'O'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'V  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '&'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'Q  +75 h  .t\CS.seek 0ml C'h'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'"% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'E'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '~' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C': '\  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C']'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'k'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'x'4 +507 h '4 #t\Flushing upper manifold C'a& h &W C'|% h %4 .t\CSV.dial :flush C'2  +37 h  .t\CS.seek 5ml C'v'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +37 h @ C')% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'p +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C'W'4 +507 h '4 C'S& h &W C'>% h %4 .t\CSV.dial :flush C'Z  +38 h  .t\CS.seek 3ml C'4'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'7% h %0 .t\CS.seek 5ml C'8'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'z'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'-'0 +507 h '0 .t\CSV.dial :waste C'I +75 h .t\CS.seek 0ml C'.'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'?& h &W C'% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'% @ +38 h @ C'Y% h %p .t\CS.seek 7ml C'l'i +416 h 'i ."connect"\CSV.dial CTV C'+  .t\CTV.dial CSV C'r  +38 h  +113  .t\CBV.dial :waste1 C'H @ +37 h @ .t\CS.seek 0ml C'N'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'c& h &W C'$% h %4 .t\CSV.dial :flush C'[  +75 h  .t\CS.seek 5ml C'`'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'g% h %p .t\CS.seek 7ml C'*'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'T  +75 h  .t\CS.seek 0ml C'('4 +766 h '4 #tDisinfecting external Intake with kill C'd& h &W C'K% h %4 .t\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'e' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +38 h +75 .t\CTV.dial :intake C'm  +76 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C's  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +606 h '4 .t\CSV.dial CSR C'i  +113 h  C'M% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'5'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&L& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.seek CC M&=' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'6( +416 h ( M&D& h &ӷ M&J% h % .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &C(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &0'η +91 h 'η .tHand.open M&B1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&P& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &^(- +6 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.seek :garage M&o'η +91 h 'η .tHand.close M&\\/ +150 h / M&& h &- M&f% h %η .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &R(ӷ +265 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &F& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &#' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'w& h &W .tHand.open M&;1 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'V& h &W C'y% h %4 .t\CSV.dial CSR C'O  +112 h  C'&% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'Q% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '"'4 +2009 h '4 #t\Purging external Intake C'h& h &W C'E% h %4 .t\CSV.dial :flush C'\  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ':' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +75 h .t\CTV.dial :intake C'~  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C']'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'k& h &W C'a% h %4 .t\CSV.dial :flush C'|  +75 h  .t\CS.seek 5ml C'2'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'x @ +37 h @ C'b% h %p .t\CS.seek 7ml C')'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'v +113 h .t\CTV.dial :air .\CS.seek 0ml C'p'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'!'0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'S% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'Z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'% h %0 .t\CS.seek 5ml C'7'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'4'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'8'0 +507 h '0 .t\CSV.dial :waste C'z +75 h .t\CS.seek 0ml C'I'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&-& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &?(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&%/ +150 h / .t\CC.seek :open C'Y( +416 h ( M&'& h &ӷ M&+% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&l& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &c& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &$'η +91 h 'η .tHand.open M&[1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &<'\ +91 h '\  #t\Completed [mbaPhase] begun 14:00:46 #\Preparing to sleep until Thu Oct 25 14:00:46 +0200 2018 #"shutdown"deferred until MFBPCR finish #tWaiting for MFBPCR til 14:00CEST25-Oct-18 #\Continuing at 14:00CEST25-Oct-18 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 3: mbaPhase\\n/home/esp/esp2/mission/18tundeployv4.rb:127\\n{1 => 0, 2 => 12, 3 => 10, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:34:22CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Waiting until 14:00CEST25-Oct-18" +280002 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2315h300ml.pcr F*g% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*T % +86 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *d'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*m% h %n ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*s  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *M'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*A  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F*D% h % ."MFBPCR"\AS.seek 0.81ml F*J'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*B% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*U  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2318h300ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ARV.dial :carrier F*G  +11 h  F*F% h %V ."MFBPCR"\AS.seek 0.5ml F*^'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*V  +22 h ."MFBPCR"delay 2 seconds +200 F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*&  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*5' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*Q  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.536ml F*E'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +107 h  ."MFBPCR"delay 7 seconds +700 F*"% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*~ n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*k  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*a% h %\  ."MFBPCR"\AS.seek 0.65ml F*|' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*]  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*v  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2318h300ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*!  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *>'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*W% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*z  +21 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*9  +65 h  F*%% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*c  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*$% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F**% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2320h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ARV.dial :carrier F*M  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*@ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*/  +22 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *X'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*D I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*=' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*,' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*B  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.536ml F*U'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*P n +21 h n ."MFBPCR"delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*0  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*f  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*o% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2320h300ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*^ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*6  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*w  +151 h F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *V' +424 h ' ."MFBPCR"\ATV.dial :ASV F*y n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*;  +43 h  F*&% h % ."MFBPCR"\AS.seek 0.62ml F*#'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*O n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*~% h % ."MFBPCR"\AS.seek 0.71ml F*:' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h % H ."MFBPCR"\AS.seek 0.735ml F*a' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*]% h % Ű ."MFBPCR"\AS.seek 0.72ml F*x' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*b n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*|% h % zG ."MFBPCR"\AS.seek 0.365ml F*v' +7161 h ' ."MFBPCR"\ATV.dial :waste F*) n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*p % +43 h % F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*S% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %  ."MFBPCR"\AS.seek 0.604ml F*>'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*7% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*z n +86 h n ."MFBPCR"\ASV.dial :wasteR F*8  +22 h  ."MFBPCR"\AS.seek 0ml F*4'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *9'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*% % +107 h % F*-% h %V ."MFBPCR"\AS.seek 0.01ml F*R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*}  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*'' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h % q ."MFBPCR"\AS.seek 0.605ml F*c'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*[% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*l' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*r n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*$  +22 h  ."MFBPCR"\AS.seek 0ml F*N'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*t  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *T' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F** n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b * '^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*d'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*( n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*m I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*i% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*@ n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *L' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*A  +43 h  F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*X  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *J'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*= n +65 h n F*B% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *q' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*, I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*P n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *_'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*0'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*\\  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*{ % +43 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*F'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %0 ."MFBPCR"\AS.seek 0.985ml F*G' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*1% h % ."MFBPCR"\AS.seek 0.97ml F*n'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*y I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *&'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Q'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *h' +223 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *5' +9987 h ' ."MFBPCR"\ATV.dial AIV F*\ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.seek 0ml F*~'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*"% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*k  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*b% h %Z ."MFBPCR"\AS.seek 0.81ml F*|'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +21 h F*2% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*S  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*W' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*8  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*-% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*R'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +21 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*?  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*` % +108 h % ."MFBPCR"\AS.seek 0ml F*('V +2547 h 'V ."shutdown"\AS.coast .\SS.coast F*5 S)5 ."shutdown"\CC.coast .\CS.coast C'6 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast M&5 ."shutdown"\Elbow.coast +2 P%5 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown .GoodNews.suppressMail "MFBPCR completed",Subject: "Shutdown" @CEST1540468846.33 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) ) h  C' ' h  P% %d h  F* *m h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ % *\ % 0\ C' 2\ F*\ 9\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'e& S)#.0X 0, 2 => 12, 3 => 10, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:35:03CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tundeployv4 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED Superphase (phase #5) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&G1 +150 h 1 M&y& h &M M&n% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &;(- +166 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &O'η +91 h 'η .tHand.close M&#/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +264 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &E' +91 h ' .t\CC.seek :closed C'"(W +416 h (W C'& h &W .tHand.open M&~1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'a  +151 h  C'b% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'x% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ')'[ +462 h '[ .t\CSV.dial :waste C'| +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Purging external Intake C'2& h &W C'v% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'S' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'! 'j  h +75 .t\CTV.dial :intake C'  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'W'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'Z& h &W C'7% h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C'8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'4% h %p .t\CS.seek 7ml C'z'i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'% +113 h .t\CTV.dial :air .\CS.seek 0ml C'-'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'} +38 h .t\CS.seek 0ml C''4 +506 h '4 C'9& h &W C'+% h %4 .t\CSV.dial :flush C'.  +38 h  .t\CS.seek 3ml C'R'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'? @ +38 h @ C''% h %0 .t\CS.seek 5ml C'['p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'c'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +507 h '0 .t\CSV.dial :waste C'$ +75 h .t\CS.seek 0ml C'l'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&N& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &g(ӷ +6 h (ӷ M&t& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&*' +91 h ' .tHand.close M&T/ +150 h / .t\CC.seek :open C'( +416 h ( M&K& h &ӷ M&`% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&(1 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +91 h '\  #t\Starting mfb #pcrBAC sampling at most 300ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 3 at 10:35:08CEST18-Oct-18\\n{1 => 0, 2 => 12, 3 => 10, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:35:03CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" F*<% #tLoading sh1 puck from tube 3 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'^& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* M&% h %\  A(% h %X A(d. h ., .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (m( +7 F* % h %&K ."readyMFB"\ATV.dial :waste2 F* % h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +194 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (e& h & M&s& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &i( +166 h ( M&M& h & A(@& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h & .t\SE.seek 8.71 Pucks below Top Plate A(' +175 h ' A(L& h & M&/& h & .t\Elbow.seek 3 M&A( +7 h ( M&D& h & A(& h & .t\Forearm.seek 3 + 300 counts M&X'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &=/ +150 h / ."downElevator"\SE.seek :down M& & A(B'X h & M&J% h %B .t\Forearm.seek :retract M&q'\ +91 h '\  .t\Elbow.seek CC M&(ӷ +84 h 'X ."downElevator"\SE.coast A(5 +58 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &C' +91 h ' .t\CC.seek :closed C'6(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  .t\SC.coast #collectSample 300ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S){+ +7 h + S)0% h % C'1& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.seek 0ml C'  S)W' +6 h ' .t\SSV.dial CBV,avoiding: :exhaust S)4  +144 h  +7  S)8% h % .t\CBV.dial between SSV and waste2 C'%  +19 h  C'z% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'-  +37 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '}'4 +2610 h '4 C'% h %4 S)9% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'+ @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'79 h 9Td +100 C'9 h 9kd +100 C'>9 h 9d +100 C'9 h 9d +100 C'49 h 9d +100 C'89 h 9d +100 C'%9 h 9d +100 C'z9 h 9d +100 C'-9 h 9 d +100 C'p9 h 9 d +100 C'Z9 h 96d +100 C'9 h 9Ld +100 C'99 h 9bd +100 C'+9 h 9xd +100 C'W9 h 9d +100 C'9 h 9d +100 C'?9 h 9d +100 C''9 h 9d +100 C'.9 h 9d +100 C'R9 h 9d +100 C'c9 h 9d +100 C'$9 h 9(d +100 C'N9 h 9>d +100 C'[9 h 9Td +100 C'9 h 9id +100 C'}9 h 9d +100 C't9 h 9d +100 C'r9 h 9d +100 C'T9 h 9d +100 C':9 h 9d +100 C'"9 h 9d +100 C'`9 h 9  d +100 C'H9 h 9 d +100 C'h9 h 9 *d +100 C'*9 h 9 ?d +100 C'I9 h 9 Td +100 C'(9 h 9 j d +100 C'K9 h 9 $d +100 C'g9 h 9 (d +100 C' 9 h 9 ,d +100 C'<9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'd9 h 9 '8 +21 h '8 M&& h & A(4& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &8' +22 h ' .t\SE.seek 12.04 Pucks below Top Plate A(%'3 +36 h '3 M&z& h & M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +90 h '\  .t\Elbow.seek CC M&Z(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'( +416 h ( A(?& h &  A('& h &  M&.& h &ӷ M&R% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &$( +143 h ( M&N& h & A([& h &  .t\Elbow.reconfigure LimpElbow M&$ ,(#b .t\Forearm.seek 2 M&' +92 h '  .t\Elbow.hold M&}, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &t1 +150 h 1 M&r% h %  A(T& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&:'8 +21 h '8 M&"& h & A(`& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &H' +22 h ' A(h% h %3 ."downElevator"\SE.seek :down A(*'X +100 (I% h % M&(% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +43 h 'X ."downElevator"\SE.coast A(5 +46 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'g @ +19 h @ .t\CS.seek 10ml C' ' +7 h ' .t\CSV.dial between air and CTV C'< ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&d1 +2 h 1 M& & h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&3(- +166 h (- M&s& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M& 'η +90 h 'η .tHand.close M&^/ +150 h / M&M& h &- M&@% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &D' +91 h ' .t\CC.seek :closed C'Y(W +416 h (W C'L& h &W .tHand.open M&=1 +150 h 1 M&e% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &_'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%J' +1598 h ' .t\PSV.dial PSR P%o +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P% ' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'X  P%  h +76 .t\PSV.dial :mixloop P%A +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%q  h .t\PS.seek 0ml P%B' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%0 +38 h .t\PS.seek 0ml P%1' +2610 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%m' +2610 h ' .t\PSV.dial :mixloop P%n +38 h .t\PS.seek 0ml P%{' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&P& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &C(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C',( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&#'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &\ 'η +91 h 'η .tHand.open M&F1 +150 h 1 M&y% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 3 into Collection Clamp C'k& h & M&% h %\  A(% h %X A(a. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (E& h & M&f& h &- M&x% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &|( +166 h ( M&)& h & A(v& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(b& h & .t\SE.seek 7.71 Pucks below Top Plate A(j' +184 h ' A(S& h & M&U& h & .t\Elbow.seek 3 M&7( +7 h ( M&& h & A(& h & .t\Forearm.seek 3 + 300 counts M&4'B +22 h 'B .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &z/ +150 h / ."downElevator"\SE.seek :down M&-& A(2'X h & M&]% h %B .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.seek CC M&!(ӷ +94 h 'X ."downElevator"\SE.coast A(5 +49 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'l(W +415 h (W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'p  +151 h  .t\CSV.dial CTV C'6  +19 h  .t\CS.seek 5ml C'?'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C''' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'.'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%R  +38 h  .t\PS.seek 2.5ml P%N' +2610 h ' .t\PSV.dial :mixloop P%[ +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%+  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Z +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%t  +150 h  .t\PS.seek :empty P%r' +2610 h ' .t\CBV.dial :waste1 C'T @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'"  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'` @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'h  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'c ` +18 h ` .t\CSV.dial :air C'$ @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'(  +38 h  .t\CS.seek 0ml C'g'4 +2647 h '4 .t\CBV.dial :waste1 C'< @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 2 M&d% h %\  A( % h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (:& h &  M&%& h &ӷ M&H% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &s( +142 h ( M&& h & A(*& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &~' +90 h ' A(O& h &  .t\SE.seek 11.71 Pucks below Top Plate A(}'Ɛ +145 h 'Ɛ M&^& h & A(M& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &@' +26 h '  .t\Elbow.hold M&K, +6 h , .t\Elbow.reconfigure TightElbow A( - h -+Ɛ M&$&.,(\ #b .t\SE.seek 12.04 Pucks below Top Plate A(u'3 +27 h '3 M&/% h %  A(3& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'8 +20 h '8 M&& h & A(L& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &=' +22 h ' .t\SE.seek 13.04 Pucks below Top Plate A(e'" +37 h '" M&& h & M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&i& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&J' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'X( +416 h ( A(A& h &  A(q& h &  M& & h &ӷ M&% h % .t\Forearm.seek :retract M&B'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &0( +143 h ( M&& h & A(& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &1' +92 h '  .t\Elbow.hold M&n, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &m1 +150 h 1 M&P% h %  A(&& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts + M&V'8 +21 h '8 M&G& h & A(\\& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &_' +22 h ' A(Y% h %" ."downElevator"\SE.seek :down A('X +100 (% h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +33 h 'X ."downElevator"\SE.coast A(5 +57 h '\  .t\SC.coast A(6 .t\PSV.dial :air P%;  +113 h  P%C% h % .t\PS.seek 0.499ml P%F' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%y +38 h .t\PRV.dial :air P%#  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%k  +37 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%a  +19 h  P%E% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %f' +250 h ' .t\PRV.dial :waste2 P%x @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %\ ' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C')& h & .tHand.open M&v1 +2 h 1 M&b& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&S(- +166 h (- M&U& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&Q/ +150 h / M&z& h &- M&-% h %η .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &9' +91 h ' .t\CC.seek :closed C'4(W +416 h (W C'j& h &W .tHand.open M&71 +150 h 1 M&2% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C',& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%8 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%p  +75 h  .tdelay 2 seconds +200 P%6% h % .t\PS.seek 1.499ml P%>' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%?' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%' +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%R  +38 h  .tdelay 2 seconds +200 P%{% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %['r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%+  +76 h .tdelay 2 seconds +200 P%Z% h %r .t\PS.seek 0.475ml P%t' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%N  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%T'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%"  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%`  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %h' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%c +75 h .t\PTV.dial :PRV,avoiding: :puck P%$ @ +76 h @ .t\PS.seek 0ml P%.' +2609 h ' .t\PSV.dial :air P%(  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%< +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%d' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%:% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %%' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'H & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %*'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'O { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %!' +1313 h ' .t\PSV.dial :air P%^  +37 h  .t\PS.seek 2.5ml P%M' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%/' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '3& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'W& ."MFBPCR"\ASV.dial :SPEdelivery h &W F* @@T .t\AWV.select :air F*L  .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P% % h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%~' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*s I +129 h I F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *@'6 +66 .t\PSV.dial :waste1 P%i  +38 h  .t\PS.seek 0ml P%o' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%u' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%= +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P%D +21 h  F*l% h %6 ."MFBPCR"\AS.seek 0.329ml F*A'ͯ +92 h P%q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%J' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +22 h  F*m% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *P' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%G' +217 ."MFBPCR"\ATV.dial :AIV F*\\ I +42 h I F*Y% h % ."MFBPCR"\AS.seek 0.469ml F*B' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%1% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%V' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%;' +739 h ' ."MFBPCR"delay 5 seconds +500 F*C9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*_ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P%r +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%# +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%k' +2610 h ' .t\PSV.dial PSR P% +112 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%F' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%x +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%f' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%v +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %b'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %U' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *n'V +850 h ' .t\PSV.dial :air P%Q  +112 h  .t\PS.seek 2.5ml P%z' +2610 h ' .t\PSV.dial :mixloop P%- +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %5' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *]'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*X I +43 h I F*j% h %Q ."MFBPCR"\AS.seek 0.01ml F*7' +171 h ' .t\PSV.dial :mixloop P%2 +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %,' +619 h ' ."MFBPCR"\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.seek :empty F*9'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*p% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *6' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*\  +43 h F*>% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b * '_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C''% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'R'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'{' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'?  '+  +37 h  +113  .t\CBV.dial :waste1 C'Z @ +38 h @ .t\CS.seek 0ml C'['4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*N  +22 h ."MFBPCR"delay 3 seconds +300 F*t% h %_ ."MFBPCR"\AS.seek 0.434ml F*"'ݏ +110 h '4 .t\CSV.dial CSR C'`  +38 h  C'T% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'c'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*$  +22 h F*h% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*('& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*. n +108 h n F*<% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C' '4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&:& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +7 h (ӷ M&H& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&%' +91 h ' .tHand.close M&O/ +150 h / .t\CC.seek :open C'*( +416 h ( M&^& h &ӷ M&!% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &M(- +264 h (- M&3& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &W& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &L'η +91 h 'η .tHand.open M&1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 3 into Collection Clamp C's& h & M&}% h %\  A(i% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (~( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&o& h &- M&=% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &u( +166 h ( M&& h & A(l& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &D' +90 h ' A(q& h & ."WCR"\SE.seek 6.71 Pucks below Top Plate A(@' +195 h ' A(K& h & M&& h & ."WCR"\Elbow.seek 3 M&A( +7 h ( M&& h & A(& h & ."WCR"\Forearm.seek 3 + 300 counts M&m'B +22 h 'B ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +59 h '&0 ."MFBPCR"delay 3 seconds +91 h / ."downElevator"\SE.seek :down M&&& A('X h & M&\\% h %B ."WCR"\Forearm.seek :retract M&Y'\ +91 h '\  ."WCR"\Elbow.seek CC M&J(ӷ +105 h 'X ."downElevator"\SE.coast A(5 +13 ."MFBPCR"\ATV.dial :waste2 F*P % +24 h (ӷ M&0& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +19 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *1'R +72 h ' ."WCR"\CC.seek :closed C'(W +416 h (W ."WCR"Hand.open M&e1 +71 h 'R ."MFBPCR"\ATV.dial :air2 F*V  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *C' +57 h 1 M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)B+ +7 h + S)r% h % C';& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'#  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +21 h  ."MFBPCR"\AS.seek 0.3ml F*6': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*' % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*R  +87 h F*?% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *+'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*Z I +43 h I F*{% h %N ."MFBPCR"\AS.seek 0.109ml F*N'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*t n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*`  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *T% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b * ' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *[% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *$' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*h% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *"' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*.% h % ."MFBPCR"\AS.seek 0.279ml F*<'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*( n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *c'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*: I +21 h I F*H% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*O % +21 h % F*|% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*^% h % ."MFBPCR"\AS.seek 0.769ml F*!' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h % q ."MFBPCR"\AS.seek 0.794ml F*W' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F* '  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *s' +4416 h ' ."MFBPCR"\ATV.dial :waste F*} n +129 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *o' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*L% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %  ."MFBPCR"\AS.seek 0.604ml F*M'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*/  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *\\'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*u % +108 h % F*D% h %V ."MFBPCR"\AS.seek 0.59ml F*0' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*G n +108 h n ."MFBPCR"\ASV.dial :wasteR F*P% h %  ."MFBPCR"\AS.seek 0.09ml F*@' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*m  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*A I +86 h I F*% h % ."MFBPCR"\AS.seek 0.12ml F*Y' +625 h ' ."MFBPCR"\ATV.dial :air3 F*I % +21 h % F**% h % ."MFBPCR"\AS.seek 0.13ml F*V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*_% h % ."MFBPCR"\AS.seek 0.23ml F*r' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h % ."MFBPCR"\AS.seek 0.255ml F*#'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*~% h %X ."MFBPCR"\AS.seek 0.24ml F*B' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *E% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *a'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C .delay 3:00 F*y @F@ h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*v'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*x'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*)% h %\  ."MFBPCR"\AS.seek 0.5ml F*Q'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*f% h %(A ."MFBPCR"\AS.seek 0.6ml F*z'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*5'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *U' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*- % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +3472 h 'V ."MFBPCR"\SPE.coast F*X  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2515h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*j  +64 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*C n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *p'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*7  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\  +21 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *9'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*6 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*'% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*R n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*g' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Z  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*+'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*t  +108 h  ."MFBPCR"delay 7 seconds +700 F*`% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*N n +22 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +42 h  ."MFBPCR"\ASV.dial :waste1 F*(  +65 h ."MFBPCR"\ARV.dial :air1 F*<  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +226243 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'H  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'O' +910 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'| @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'4  +94 h  C'^% h % ."WCR"\CSV.dial between air and CTV C' ` +19 h ` ."WCR"\CS.seek 7.288ml C'%' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'3  '!  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'd'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'}% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C's @ +75 h @ +50 S)i% h % ."WCR"\CBV.dial between CSV and waste2b C'=  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'o ` +57 h ` ."WCR"\CS.seek 7.288ml C'l' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'q  '  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'K'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'M' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'/  +38 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +816 h ' #"WCR"\Pumping wcr puck from tube 3 up to 55psi... .\CBV.dial SSV C'u @ +75 h @ +50 S)D% h % ."WCR"\CBV.dial between CSV and waste2b C'\\  +95 h  C'G% h % ."WCR"\CSV.dial between air and CTV C'P ` +56 h ` ."WCR"\CS.seek 7.288ml C'0' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'A  'm  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'@  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'I' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'*  +151 h C'Y& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 2 M&e% h %\  A(_% h %X A(V. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (;( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (1& h &  M&r& h &ӷ M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &~( +142 h ( M&#& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(B& h &  ."WCR"\SE.seek 12.71 Pucks below Top Plate A(E' +136 h ' M&k& h & A(a& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 2 M&#Q@2^d^2b &' +25 h '  ."WCR"\Elbow.hold M&F, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(v- h -+ ."WCR"\SE.seek 13.04 Pucks below Top Plate A('" +27 h '" M&b% h %  A(x& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&)'8 +21 h '8 M&& h & A(f& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &Q' +22 h ' ."WCR"\SE.seek 14.04 Pucks below Top Plate A(' +36 h ' M&z& h & M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  ."WCR"\Elbow.seek CC M&-(ӷ +142 h (ӷ M&U& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ ."WCR"\Forearm.seek CC M&j' +91 h ' ."WCR"Hand.close M&2/ +150 h / ."WCR"\CC.seek :open C']( +416 h ( A(n& h &  A(S& h &  M&C& h &ӷ M&8% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &7( +143 h ( M&\ & h & A(>& h &  ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &p' +92 h '  ."WCR"\Elbow.hold M&6, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &'1 +150 h 1 M&9% h %  A(R& h &  ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 2 + 300 counts + M&?'8 +21 h '8 M&& h & A(Z& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &{' +22 h ' A(g% h % ."downElevator"\SE.seek :down A(t'X +100 (`% h %v M&+% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +22 h 'X ."downElevator"\SE.coast A(5 +67 h '\  ."WCR"\SC.coast #\Starting finalPurge A(6 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'[& h & .tHand.open M&T1 +2 h 1 M&h& h & M&$% h %\  .t\Elbow.seek FlushPuck::Garage M& (- +166 h (- M&"& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&<'η +91 h 'η .tHand.close M&/ +150 h / M&.& h &- M&c% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &|' +91 h ' .t\CC.seek :closed C'(W +415 h (W C'4& h &W .tHand.open M&^1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'3& h &W C'!% h % .t\CSV.dial CSR C'%  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'd'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +463 h '[ .t\CSV.dial :waste C'} +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'W'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C's% h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '='p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'o% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'q  +37 h  +113  .t\CBV.dial :waste1 C'l @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'K& h &W C'% h %4 .t\CSV.dial :flush C'/  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'M @ +38 h @ C' % h %p .t\CS.seek 7ml C'u'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'D  +37 h  .t\CBV.dial :waste2b C'\\  +76 h  .t\CS.seek 0ml C'G'4 +765 h '4 #t\Flushing upper manifold C'P& h &W C'&% h %4 .t\CSV.dial :flush C'm  +76 h  .t\CS.seek 5ml C'A'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'@% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'* 'Y  +37 h +76 .t\CTV.dial :air C'e  +150 h .t\CS.seek 0ml C'_'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C'1 +37 h .t\CS.seek 0ml C'r'4 +507 h '4 C'& h &W C'#% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'B'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'J @ +38 h @ C'k% h %0 .t\CS.seek 5ml C'a'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'v'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'b'0 +507 h '0 .t\CSV.dial :waste C'x +75 h .t\CS.seek 0ml C''4 +506 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'f% h %4 .t\CSV.dial CSR C'z  +151 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'~' +416 h ' ."connect"\CSV.dial CBV C'E .t\CBV.dial CSV +113 h .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'U' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'X  +76 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ')'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'Q% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'F'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '5  +37 h  +113  .t\CBV.dial :waste1 C'n @ +38 h @ .t\CS.seek 0ml C'S'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'C  +37 h  C'8% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '\ 'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '>' +709 h ' ."connect"\CSV.dial CTV C'j  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'-  +75 h  .t\CS.seek 0ml C'''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'9  +38 h  C'R% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'g '  +38 h +75 .t\CTV.dial :air C'7  +151 h .t\CS.seek 0ml C'`'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +507 h '0 .t\CSV.dial :waste C'[ +38 h .t\CS.seek 0ml C'T'4 +506 h '4 #t\Flushing upper manifold C'h& h &W C'$% h %4 .t\CSV.dial :flush C'p  +38 h  .t\CS.seek 5ml C'?'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'"% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CBV C'{ .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C't'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C';'0 +507 h '0 .t\CSV.dial :waste C'6 +38 h .t\CS.seek 0ml C''4 +506 h '4 C'.& h &W C'c% h %4 .t\CSV.dial :flush C'N  +38 h  .t\CS.seek 3ml C'<'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C' % h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C':'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C'4 +75 h .t\CS.seek 0ml C'^'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'3% h %4 .t\CSV.dial :flush C'!  +37 h  .t\CS.seek 5ml C'%'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'|% h %p .t\CS.seek 7ml C'I'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'd  +38 h  +113  .t\CBV.dial :waste1 C'} @ +38 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C's% h %4 .t\CSV.dial :flush C'i  +75 h  .t\CS.seek 5ml C'W'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'o% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'q  +76 h  .t\CS.seek 0ml C'l'4 +766 h '4 #tDisinfecting external Intake with kill C'& h &W C'K% h %4 .t\CSV.dial CSR C'  +37 h  C'/% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C''L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'M' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +37 h +76 .t\CTV.dial :intake C'D  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'\\' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'u  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'P'4 +607 h '4 .t\CSV.dial CSR C'&  +112 h  C'm% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'G'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'0' +1373 h ' .t\CSV.dial CBV C'@ +112 h .t\CS.seek 0ml C'A'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +6 h (ӷ M&e& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&1/ +150 h / .t\CC.seek :open C'V( +416 h ( M&& h &ӷ M&#% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &r(- +264 h (- M&J& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &B'η +91 h 'η .tHand.open M&a1 +150 h 1 M&v% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'b& h & .tHand.open M&1 +2 h 1 M&f& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &z(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&E/ +150 h / M&& h &- M&~% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &U(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &)' +91 h ' .t\CC.seek :closed C'F(W +416 h (W C'& h &W .tHand.open M&51 +150 h 1 M&n% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'C& h &W C'8% h %4 .t\CSV.dial CSR C'S  +113 h  C'\ % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'j'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '-% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '>'[ +463 h '[ .t\CSV.dial :waste C'9 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'R'4 +2010 h '4 #t\Purging external Intake C''& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'7 +75 h .t\CTV.dial :intake C'Z  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'`'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'[  +75 h #t\Flushing upper manifold C'+& h &W C'h% h %4 .t\CSV.dial :flush C'$  +76 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'% h %p .t\CS.seek 7ml C'"'i +416 h 'i ."connect"\CSV.dial CBV C'? .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'{'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'('0 +506 h '0 .t\CSV.dial :waste C't +38 h .t\CS.seek 0ml C'6'4 +506 h '4 C';& h &W C'.% h %4 .t\CSV.dial :flush C'c  +38 h  .t\CS.seek 3ml C'N'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C' 'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'<'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C': +75 h .t\CS.seek 0ml C'4'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&]& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'|( +416 h ( M&%& h &ӷ M& % h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &}(- +265 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &s'η +91 h 'η .tHand.open M&i1 +150 h 1 M& % h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +90 h '\  #t\Completed [mbaPhase] begun 14:01:01 #\Extending 18tundeployv4 until completion of MFBPCR +280002 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss18oct2515h300ml.pcr F*o% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*/'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +65 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*H% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*#'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*k'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*v'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2518h300ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*Q% h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*n  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *S'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*\  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*)% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*-  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*9' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*> % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*'% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*R% h %< ."MFBPCR"\AS.seek 0.594ml F*7'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*+  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*$' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*`  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*T  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*p'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2518h300ml.pcr F*{% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*(  +21 h ."MFBPCR"\ATV.dial :air3 F*; % +87 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*<'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*W'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*K  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *&'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2520h300ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +10 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*H  +22 h ."MFBPCR"delay 2 seconds +200 F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*O% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*J % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*#% h % ."MFBPCR"\AS.seek 0.53ml F**' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*a  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +21 h  ."MFBPCR"delay 7 seconds +700 F*b% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*f n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*B  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*r  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*x % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc18oct2520h300ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*z @@T ."MFBPCR"\ASV.dial :SPEdelivery F*V  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *C' +424 h ' ."MFBPCR"\ATV.dial :ASV F*8 n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*5  +43 h  F*\ % h % ."MFBPCR"\AS.seek 0.62ml F*)'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*S n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *-'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *j'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*A'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*> % +43 h % F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *9' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.71ml F*g' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h % H ."MFBPCR"\AS.seek 0.735ml F*h' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h % Ű ."MFBPCR"\AS.seek 0.72ml F*T' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*$% h % zG ."MFBPCR"\AS.seek 0.365ml F*?' +7161 h ' ."MFBPCR"\ATV.dial :waste F*" n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *y'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*p% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*.% h % ."MFBPCR"\AS.seek 0.579ml F*t' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*c'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*: n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*<'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *!'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*]% h %V ."MFBPCR"\AS.seek 0.01ml F*U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*F% h % ."MFBPCR"\AS.seek 0.58ml F*%' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*i% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*I' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*d n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*s  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *q' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*= n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*K n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*l n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*D I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\\  +43 h  F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *m': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*P  +65 h  F*&% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *G'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*0 n +65 h n F*@% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Y' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*1 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *O': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*J% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *#'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*_ n +64 h n F*a% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *k' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F** I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*b  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*f n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*B'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*E  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +42 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *r' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*Q'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*n% h % ."MFBPCR"\AS.seek 0.97ml F*X'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*8 I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\ 'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *S'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *-'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*j  +43 h  F*>% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *A' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*9' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*Z'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*R% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*+  +22 h ."MFBPCR"\ATV.dial :air3 F*g % +43 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *`'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*$'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*p% h %n ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*;  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*.  +65 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *6'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*U'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*W % +108 h % ."MFBPCR"\AS.seek 0ml F*l'V +2547 h 'V #t\Asleep after completing 18tundeployv4 assays .GoodNews.suppressMail "{1 => 0, 2 => 15, 3 => 7, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:36:08CEST18-Oct-18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tundeployv4 assays" #\Mission runtime was 12:15.304234 !o:SignalException :\ signoi:bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" SIGHUP:@timestampu: Time 5_ !o:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error #m\EXIT @1539851859.29CEST @1542640339.35CET = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiP!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 23:50:24CEST25-Oct-18 .delayUntil "23:50:24.00CEST25-Oct-18" @CEST1540504224.00 #-> execute '18tun_PostDeploy.rb' !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"FNo '18tun_PostDeploy.rb' script found on /home/esp/esp2/mission:. #-> execute '18tun_postDeploy.rb' !o:RuntimeError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+Mission's startTube should be 3, not 4 #-> exit #m\EXIT @1542640403.04CET @1542640430.61CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:13:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: postDeployPhase\\n/home/esp/esp2/mission/18tun_postDeploy.rb:105\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:14:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:14:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy phase postDeployPhase" #\Phase [postDeployPhase] #==================> STARTED postDeploycoreNegNTC (phase #1) <=== #\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 4 at 16:14:24CET19-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:14:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down A(H'X M&I& h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'T& h &W ."airStroke"\CSV.dial :waste S)U% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'V'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9d +100 C'M9 h 9 d +100 C'N9 h 9$d +100 C'F9 h 9(d +100 C'@9 h 9 ,d +100 C'H9 h 9$0d +100 C'C9 h 9<4d +100 C'K9 h 9S8d +100 C'.9 h 9kd +100 C'e9 h 9Td +100 C'f9 h 9kd +100 C'g9 h 9d +100 C'h9 h 9d +100 C'i9 h 9d +100 C'_9 h 9d +100 C'Y9 h 9d +100 C'k9 h 9d +100 C'l9 h 9 d +100 C'm9 h 9 d +100 C'd9 h 96d +100 C'o9 h 9Ld +100 C'`9 h 9bd +100 C'q9 h 9xd +100 C'r9 h 9d +100 C's9 h 9d +100 C'n9 h 9d +100 C't9 h 9d +100 C'v9 h 9d +100 C'w9 h 9d +100 C'y9 h 9d +100 C'u9 h 9(d +100 C'{9 h 9>d +100 C'j9 h 9Td +100 C'z9 h 9id +100 C'x9 h 9d +100 C'|9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C' 9 h 9 $d +100 C'\ 9 h 9 (d +100 C' 9 h 9 ,d +100 C' 9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9   +18 h C'M% h %4 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'F  +37 h C'@% h %p .t\CS.seek 7ml C'H'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'C @ +37 h @ .t\CS.seek 10ml C'K' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'. ` +132 h ` #tLysing Complete C'Q& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&R% h %\  A(T% h %X A(U. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (O& M&8& h &ӷ & .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit A( 'Z .t\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C']( +23 h 'Z +393 ( A(^& h & A(W& h & M&4& h &ӷ M&a% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +142 h ( M&e& h & A(f& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &g' +93 h ' .t\Elbow.hold M&h, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&_% h % A(Y& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&k'B +20 h 'B M&l& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(o% h %Z ."downElevator"\SE.seek :down A(`'X +100 (q% h % M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'n @ +2 A(6 +17 h @ .t\CS.seek 10ml C't' +6 h ' .t\CSV.dial between air and CTV C'v ` +19 h ` .t\AIV.dial :bypass C'w { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'y& h & .tHand.open M&u1 +2 h 1 M&{& h & M&j% h %\  .t\Elbow.seek FlushPuck::Garage M&z(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&}'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&\ 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +112 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.seek 2.5ml P%+' +2610 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +6 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'%( +416 h ( M&2& h &ӷ M&0% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&$1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'<& h & M&9% h %\  A(?% h %X A(D. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (B(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &,9 M&=& h &- M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&I& h &w A(J& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(;& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(>' +66 h ' A(M& h &,9 M&#& h &w .t\Elbow.seek 4 M&F(w +6 h (w M&@& h &w A(N& h &,9 .t\Forearm.seek 4 + 300 counts M&C'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down M&H& A(.'X h &w M&Q% h %7 .t\Forearm.seek :retract M&R'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&T(ӷ +143 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'[(W +416 h (W .tHand.open M&Z1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'^'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'W' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'4'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%a  +37 h  .t\PS.seek 2.5ml P%e' +2610 h ' .t\PSV.dial :mixloop P%f +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%V  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%i  +151 h  .t\PS.seek :empty P%_' +2609 h ' .t\CBV.dial :waste1 C'Y @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'l  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'm @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'o  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'b ` +19 h ` .t\CSV.dial :air C'c @ +37 h @ .t\CS.seek 10ml C'q' +2647 h ' .t\CSV.dial CTV C'r  +38 h  .t\CS.seek 0ml C'g'4 +2647 h '4 .t\CBV.dial :waste1 C'k @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'n  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&v% h %\  A(w% h %X A(y. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (u( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ({& h & M&j& h &ӷ M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &x( +143 h ( M&|& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(K& h & .t\SE.seek -0.29 Pucks below Top Plate A(h'\\ +264 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&s, +6 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(~- h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&% h % A(z& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &}'B +20 h 'B M&]& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A( ' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h & A(& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A((& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&", +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &+1 +150 h 1 M&% h % A(*& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&/& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(2'X +100 (0% h %S M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%7  + A(6 +112 h  P%,% h % .t\PS.seek 0.499ml P%$' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%3  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%<  +37 h .t\CBV.dial AIV C'9  +38 h .t\PRV.dial between CBV and air P%?  +19 h  .t\PRV.dial :air P%D  +18 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +251 h ' .t\PRV.dial :waste2 P%) @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'I& h & .tHand.open M&J1 +2 h 1 M&;& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&M(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&:/ +150 h / M&E& h &- M&H% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'>& h &W .tHand.open M&.1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  C'%& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Z @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%O' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%B' +1094 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 0ml P%^' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%W +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%a  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %f'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%V  +76 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%i' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%e  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Y'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%l  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%m  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %o' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%b +75 h .t\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .t\PS.seek 0ml P%4' +2610 h ' .t\PSV.dial :air P%r  +75 h  .t\PS.seek 2.5ml P%q' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%k +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%n' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%v' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%w +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%y  +75 h P%{% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %j' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'd & +50 h & .t\AWV.select :waste C' +7 P%|% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %`'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'K { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%~' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'z& h &W F*] @@T .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass .\PSV.dial PSR P% F*  +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*[% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%* +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%/' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial PSR P%' +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*,9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%_ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%<' +2609 h ' .t\PSV.dial PSR P%9 +113 h P%?% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%$' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %;'k +2679 h 'k .t\PSV.dial :waste1 P%1  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *"'V +849 h ' .t\PSV.dial :air P%:  +113 h  .t\PS.seek 2.5ml P%E' +2610 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %5' +5106 h ' .t\PSV.dial :air P%N  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %&' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *Q'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*.' +171 h ' .t\PSV.dial :mixloop P%F +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +619 h ' ."MFBPCR"\ATV.dial :waste F*Z n +128 h n ."MFBPCR"\AS.seek :empty F*8'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*\\  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F*O% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'B  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'a'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'^  .t\CTV.dial CSV C'V  +38 h  +113  .t\CBV.dial :waste1 C'! @ +38 h @ .t\CS.seek 0ml C'f'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*e  +22 h ."MFBPCR"delay 3 seconds +300 F*i% h %_ ."MFBPCR"\AS.seek 0.434ml F*l'ݏ +110 h '4 .t\CSV.dial CSR C'm  +38 h  C'Y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'b'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*c  +22 h F*o% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*r'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*4 n +107 h n F*k% h %& ."MFBPCR"\AS.seek 0.092ml F*q'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'w'4 +2647 h '4 C'y& h &W #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'`( +416 h ( M&L& h &ӷ M&T% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 4 into Collection Clamp C'x& h & M&h% h %\  A(% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (X(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (}& h &,9 M&& h &- M&\ % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A([& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A(' +76 h ' A(s& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M& (w +7 h (w M&(& h &w A(& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&+'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A('X M&*& h &w M&% h %7 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' ."WCR"\CC.seek :closed C''(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +370 h (W ."WCR"Hand.open M& 1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *2' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 0ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)_% h % C'7& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'3  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +75 h @ +50 S)F% h % ."WCR"\CBV.dial between CSV and waste2b C'Q  +94 h  C'&% h % ."WCR"\CSV.dial between air and CTV C'Z ` +19 h ` ."WCR"\CS.seek 7.288ml C'.' +164 h ' ."MFBPCR"delay 5 seconds +317 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'\\  'S  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '8'4 +107 ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*O% h % ."MFBPCR"\AS.seek 0.52ml F*P' +1747 h '4 ."WCR"\CBV.dial :waste2b C'B  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'g  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'a  +38 h  C'^% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'V' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)e% h % ."WCR"\CBV.dial between CSV and waste2b C'i  +22 h ' ."MFBPCR"delay 5 seconds +72 h  C'm% h % ."WCR"\CSV.dial between air and CTV C'Y ` +57 h ` ."WCR"\CS.seek 7.288ml C't' +371 ."MFBPCR"\ATV.dial ASV F*f n +64 h n ."MFBPCR"\ASV.dial :wasteR F*c  +21 h  ."MFBPCR"\AS.seek 0.3ml F*o': +25 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'4  'l  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'k'4 +1919 h '4 ."WCR"delay 2:00 +2351 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*r'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*b % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F*y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *{'N +439 ."WCR"\CBV.dial :waste2b C'd  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '|' +41 h ' ."WCR"delay 1:00 +528 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*K I +43 h I F*G% h %N ."MFBPCR"\AS.seek 0.109ml F*R'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*C n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*L  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *T% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *j' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +79 ."WCR"\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'z  +38 h  C'v% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +27 ."MFBPCR"\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *' +574 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'h @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'p  +94 h  C'}% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'\ ' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '['4 +1919 h '4 ."WCR"delay 2:00 +1031 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*s% h % ."MFBPCR"\AS.seek 0.279ml F*'Q +933 ."WCR"\CBV.dial :waste2b C'(  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'~  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&*% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&+% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(`'K +253 h 'K M&q& h & A( & h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow A(_- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(7' +27 h ' M&3% h % A(/& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&0'B +20 h 'B M&X& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &?' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(9' +37 h ' M&<& h & M&A% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&$& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ ."WCR"\Forearm.seek CC M&J' +91 h ' ."WCR"Hand.close M&2/ +150 h / ."WCR"\CC.seek :open C'1( +415 h ( A()& h & A(@& h & M&I& h &ӷ M&:% h % ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &H( +143 h ( M&;& h & A(5& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &U' +92 h ' ."WCR"\Elbow.hold M&M, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &E1 +150 h 1 M&"% h % A(w& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&>'B +20 h 'B M&F& h & A(Q& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &&' +22 h ' A(Z% h % ."downElevator"\SE.seek :down A('X +100 (S% h %B M&\\% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'O& h & .tHand.open M&.1 +2 A(6 h 1 M&=& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&8(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&W/ +150 h / M&V& h &- M&% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +264 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &!' +91 h ' .t\CC.seek :closed C'f(W +416 h (W C'c& h &W .tHand.open M&l1 +150 h 1 M&4% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C't& h &W C'o% h % .t\CSV.dial CSR C'b  +38 h  C'r% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'd +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'K& h &W C'G% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'C'p +384 h 'Q ."MFBPCR"delay 30 seconds +266 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'T% h %p .t\CS.seek 7ml C'R'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'z  +38 h  +113  .t\CBV.dial :waste1 C'v @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'|& h &W C'j% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +63 ."MFBPCR"\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +286 .t\CSV.dial :air C'} @ +38 h @ C'% h %p .t\CS.seek 7ml C'x'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'\ '4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +16 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +22 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +15 h % F*(% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *['v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*~ I +21 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F**'H +454 h 'P .tdelay 5 seconds +171 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +86 .t\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +107 ."MFBPCR"\ATV.dial :bleach F*+  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F*' q +580 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C','4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'q  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'/  +38 h  .t\CBV.dial :waste2b C'X  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'<  +37 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C'` .t\CBV.dial CSV C'  +37 h +75 .t\CTV.dial :air C'$  +151 h .t\CS.seek 0ml C'u'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +506 h '0 .t\CSV.dial :waste C'7 +38 h .t\CS.seek 0ml C'?'4 +507 h '4 #t\Flushing upper manifold C' & h &W C'2% h %4 .t\CSV.dial :flush C'D  +37 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'@% h %p .t\CS.seek 7ml C'I'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C': +113 h .t\CTV.dial :air .\CS.seek 0ml C';'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +507 h '0 .t\CSV.dial :waste C'J +37 h .t\CS.seek 0ml C''4 +33 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +152 h '4 C'"& h &W C'w% h %4 .t\CSV.dial :flush C'F  +37 h  .t\CS.seek 3ml C'Q'0 +111 F*Z% h % q ."MFBPCR"\AS.seek 0.794ml F*#' k +396 h '0 .tdelay 5 seconds +129 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*S% h % k ."MFBPCR"\AS.seek 0.779ml F*\\'  +49 .t\CSV.dial :air C'O @ +38 h @ C'.% h %0 .t\CS.seek 5ml C'='p +237 h '  ."MFBPCR"delay 3 seconds +179 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +121 ."MFBPCR"\ATV.dial :AIV F*U I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *>' +422 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'& +76 h .t\CS.seek 0ml C'g'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'M'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'V% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'6  '^  +37 h  +113  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C'Y'4 +376 h ' ."MFBPCR"\ATV.dial :waste F*8 n +129 h n ."MFBPCR"\AS.seek :empty F*e'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*1 % +43 h % F*c% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *l' +102 h '4 #t\Flushing lower manifold's waste2b port C'4& h &W C't% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C'b'p +45 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*!% h % ."MFBPCR"\AS.seek 0.579ml F*' +384 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +37 h @ C' % h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'K  +75 h  .t\CS.seek 0ml C'G'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.seek CC M&C' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'z( +415 h ( M&v& h &ӷ M&R% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &j(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&}% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'h& h & .tHand.open M&1 +2 h 1 M& & h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &x(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&(/ +150 h / M&\ & h &- M&p% h %η .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'*(W +416 h (W C'+& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +38 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 ',% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +463 h '[ .t\CSV.dial :waste C'/ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +2010 h '4 #t\Purging external Intake C'3& h &W C'<% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +38 .t\CTV.dial :intake C'`  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'$'4 +415 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +173 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'u  +76 h #t\Flushing upper manifold C'7& h &W C'0% h %4 .t\CSV.dial :flush C'  +45 F*2% h %  ."MFBPCR"\AS.seek 0.604ml F*D'\ 48 +30 h  .t\CS.seek 5ml C'?'p +496 h '\ 48 ."MFBPCR"delay 3 seconds +154 h 'p .tdelay 5 seconds +146 ."MFBPCR"\ATV.dial :bleach F*)  +21 h F*@% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*'' +324 h '  ."MFBPCR"delay 3 seconds +9 .t\CSV.dial :air C': @ +38 h @ C'I% h %p .t\CS.seek 7ml C';'i +253 ."MFBPCR"\ATV.dial :ASV F*J n +86 h n ."MFBPCR"\ASV.dial :wasteR F*5  +43 h  ."MFBPCR"\AS.seek 0ml F*E'V +34 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'" +113 h .t\CTV.dial :air .\CS.seek 0ml C'w'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +506 h '0 .t\CSV.dial :waste C'Z +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'H% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.seek 3ml C'O'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +37 h @ C'#% h %0 .t\CS.seek 5ml C'Q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'U'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +507 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'B'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +6 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&g/ +150 h / .t\CC.seek :open C'W( +416 h ( M&V& h &ӷ M&M% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &^(- +265 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &8'η +91 h 'η .tHand.open M&11 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &4'\ +90 h '\  #t\Completed [postDeployPhase] begun 01:00:31 #\Extending 18tun_postDeploy until completion of MFBPCR +3920 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *>'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*o % +108 h % F*e% h %V ."MFBPCR"\AS.seek 0.59ml F*r' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*! n +108 h n ."MFBPCR"\ASV.dial :wasteR F*Y% h %  ."MFBPCR"\AS.seek 0.09ml F*l' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*b% h % ."MFBPCR"\AS.seek 0.12ml F*n' +625 h ' ."MFBPCR"\ATV.dial :air3 F*K % +21 h % F*d% h % ."MFBPCR"\AS.seek 0.13ml F*{' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*T% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h % ."MFBPCR"\AS.seek 0.255ml F*f'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*R% h %X ."MFBPCR"\AS.seek 0.24ml F*C' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *}'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*| @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*s  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *('\  +1032 h '\  F*\ % h %\  ."MFBPCR"\AS.seek 0.5ml F*p'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*j% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*['(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *x' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*~ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +3472 h 'V ."MFBPCR"\SPE.coast F*+  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*,  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*3  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*X  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*` % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*u  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.65ml F*@' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 F*:  +65 h ."MFBPCR"\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*'% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*w  +22 h ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +21 h F*S% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*O'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*&  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*g' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0104h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ARV.dial :carrier F*i  +11 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *f'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *k'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*}  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*h' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*~  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.65ml F*+' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*z'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*q % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0104h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *G'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +31 h  ."MFBPCR"\ATV.dial :air3 F*I % +87 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*H  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*g% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F** n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *V'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *1'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*c n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*8' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*E I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.53ml F*o' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*e % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +108 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*r'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*L  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *C'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +21 h F*h% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*[  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*$ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*I  +22 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *D'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*5 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*' n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Z  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +108 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*.% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h.pcr F*a% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F**  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*6% h %n ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +32 h  ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*n% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*d% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*f% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*R  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0111h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*+ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*| % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*,  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*< n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*X'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*`' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*A  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*u% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0111h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*7 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*0  +151 h F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +423 h ' ."MFBPCR"\ATV.dial :ASV F*I n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*?  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*5'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*" n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *''V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*J'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*@ % +43 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +423 h ' ."MFBPCR"\ATV.dial :air3 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*H' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*w% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*#% h % Ű ."MFBPCR"\AS.seek 0.72ml F*O' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*= n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*&% h % zG ."MFBPCR"\AS.seek 0.365ml F*U' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *g'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*a % +43 h % F*\\% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*V% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %  ."MFBPCR"\AS.seek 0.604ml F*m'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*9% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*E n +86 h n ."MFBPCR"\ASV.dial :wasteR F*6  +22 h  ."MFBPCR"\AS.seek 0ml F*1'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*o % +108 h % F*8% h %V ."MFBPCR"\AS.seek 0.01ml F*>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*!% h % ."MFBPCR"\AS.seek 0.58ml F*y' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h % q ."MFBPCR"\AS.seek 0.605ml F*K'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*n% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*l' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*{ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*d  +21 h  ."MFBPCR"\AS.seek 0ml F*L'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*P  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *v' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*f n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *i'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*R n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*C n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *}' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\  +43 h  F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*p  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *('Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*[ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*~  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*x  +64 h  F*|% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*,% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*/  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *z': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*< n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *G'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*3 n +151 h n ."MFBPCR"\AS.seek 0ml F*'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*_  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*X % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*`% h % ."MFBPCR"\AS.seek 0.96ml F* '0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*)% h % ."MFBPCR"\AS.seek 0.97ml F*$'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*I I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *"'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *D'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*'  +43 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Z' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *J' +9988 h ' ."MFBPCR"\ATV.dial AIV F*] I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.seek 0ml F*w'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*H% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*=% h %Z ."MFBPCR"\AS.seek 0.81ml F*&'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*U'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*\\% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +64 h  F**% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *e' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +21 h F*8% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*b  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *i'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*C'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0115h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*k  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*(' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*[  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*~ % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*x  +108 h  ."MFBPCR"delay 7 seconds +700 F*|% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*<  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0115h.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*u % +87 h % F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +21 h F*$% h %n ."MFBPCR"\AS.seek 0.82ml F*?'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *5'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +31 h  ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*Q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*='m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*O% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0117h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*6% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*r% h %Fj ."MFBPCR"\AS.seek 0.524ml F*>' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*b  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*K  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*d'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*l  +108 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*P I +107 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*i  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*k'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0117h.pcr F*s% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*}  +21 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *p'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*j% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*(% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*+  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*x% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *|'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*u% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0119h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*?  +10 h  F*5% h %V ."MFBPCR"\AS.seek 0.5ml F*''(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*;  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*H  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*S n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*. I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*Q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*# % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*O% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*\\ I +108 h I ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0119h.pcr F*c% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*t'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *l'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*k'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +21 h F*\ % h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0121h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +10 h  F*|% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*, n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*7 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*3'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*`% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*)  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*I' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*5 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0121h.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*D  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*O  +64 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *&'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' @CET82808.26 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F**  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*1% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0200h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*l% h %V ."MFBPCR"\AS.seek 0.5ml F*L'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*i' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*C % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*}  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*k'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*h  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*j I +108 h I ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*(  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*x'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0200h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*0 % +87 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +21 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +21 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *H'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *O'V +5060 h 'V #t\Asleep after completing 18tun_postDeploy assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:15:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy assays" #\Mission runtime was 2:38.707189 #-> exit #m\EXIT @1542641597.05CET @1542641857.26CET = =mquickScheduler =tquick =ECan =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refi; : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #Loading /home/esp/esp2/mission/18tun_postDeploy.rb mission .GoodNews.suppressMail "Starting 18tun_postDeploy w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:37:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: postDeployPhase\\n/home/esp/esp2/mission/18tun_postDeploy.rb:105\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:37:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:37:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy phase postDeployPhase" #\Phase [postDeployPhase] #==================> STARTED postDeploycoreNegNTC (phase #1) <=== #\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 4 at 16:37:49CET19-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:37:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB #Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil .tHand.open F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'T& h &W ."airStroke"\CSV.dial :waste S)U% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'V'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X abort! "something wrong with mission" !o:CmdServer::Client::Error:bt["mwj:1:in `topLevelBinding'" execute '18tun_postDeploy.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:53:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: postDeployPhase\\n/home/esp/esp2/mission/18tun_postDeploy.rb:105\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:53:47, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:53:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy phase postDeployPhase" #\Phase [postDeployPhase] #==================> STARTED postDeploycoreNegNTC (phase #1) <=== #\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 4 at 16:53:51CET19-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:53:51, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*/% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C'0& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* .tHand.open M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'T& h &W ."airStroke"\CSV.dial :waste S)U% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'V'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9d +100 C'M9 h 9 d +100 C'N9 h 9$d +100 C'F9 h 9(d +100 C'@9 h 9 ,d +100 C'I9 h 9$0d +100 C'C9 h 9<4d +100 C'K9 h 9S8d +100 C'.9 h 9kd +100 C'e9 h 9Td +100 C'f9 h 9kd +100 C'g9 h 9d +100 C'h9 h 9d +100 C'i9 h 9d +100 C'_9 h 9d +100 C'Y9 h 9d +100 C'k9 h 9d +100 C'l9 h 9 d +100 C'm9 h 9 d +100 C'd9 h 96d +100 C'o9 h 9Ld +100 C'`9 h 9bd +100 C'q9 h 9xd +100 C'r9 h 9d +100 C's9 h 9d +100 C'n9 h 9d +100 C't9 h 9d +100 C'v9 h 9d +100 C'w9 h 9d +100 C'y9 h 9d +100 C'u9 h 9(d +100 C'{9 h 9>d +100 C'j9 h 9Td +100 C'z9 h 9id +100 C'x9 h 9d +100 C'|9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C' 9 h 9 $d +100 C'\ 9 h 9 (d +100 C' 9 h 9 ,d +100 C' 9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9   +18 h C'M% h %4 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'F  +37 h C'@% h %p .t\CS.seek 7ml C'I'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'C @ +37 h @ .t\CS.seek 10ml C'K' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'. ` +132 h ` #tLysing Complete C'Q& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&R% h %\  A(T% h %X A(U. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (O& M&8& h & &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A( 'Z .t\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C']( +23 h 'Z +393 ( A(^& h & A(W& h & M&4& h &ӷ M&a% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +142 h ( M&e& h & A(f& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &g' +93 h ' .t\Elbow.hold M&h, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&_% h % A(Y& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &k'B +20 h 'B M&l& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(o% h %Z ."downElevator"\SE.seek :down A(`'X +100 (q% h % M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'n @ +2 A(6 +17 h @ .t\CS.seek 10ml C't' +6 h ' .t\CSV.dial between air and CTV C'v ` +19 h ` .t\AIV.dial :bypass C'w { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'y& h & .tHand.open M&u1 +2 h 1 M&{& h & M&j% h %\  .t\Elbow.seek FlushPuck::Garage M&z(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&}'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&\ 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +112 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.seek 2.5ml P%+' +2610 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +6 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'%( +416 h ( M&2& h &ӷ M&0% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&$1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'<& h & M&9% h %\  A(?% h %X A(D. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (B(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &,9 M&=& h &- M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&H& h &w A(J& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(;& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(>' +66 h ' A(M& h &,9 M&#& h &w .t\Elbow.seek 4 M&F(w +6 h (w M&@& h &w A(N& h &,9 .t\Forearm.seek 4 + 300 counts M&C'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down A(I'X M&.& h &w M&Q% h %7 .t\Forearm.seek :retract M&R'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&T(ӷ +143 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'[(W +416 h (W .tHand.open M&Z1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'^'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'W' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'4'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%a  +37 h  .t\PS.seek 2.5ml P%e' +2610 h ' .t\PSV.dial :mixloop P%f +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%V  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%i  +151 h  .t\PS.seek :empty P%_' +2609 h ' .t\CBV.dial :waste1 C'Y @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'l  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'm @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'o  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'b ` +19 h ` .t\CSV.dial :air C'c @ +37 h @ .t\CS.seek 10ml C'q' +2647 h ' .t\CSV.dial CTV C'r  +38 h  .t\CS.seek 0ml C'g'4 +2647 h '4 .t\CBV.dial :waste1 C'k @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'n  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&v% h %\  A(w% h %X A(y. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (u( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ({& h & M&j& h &ӷ M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &x( +143 h ( M&|& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(K& h & .t\SE.seek -0.29 Pucks below Top Plate A(h'\\ +264 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&s, +6 h , .t\Elbow.reconfigure TightElbow A(~- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&% h % A(z& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&}'B +20 h 'B M&]& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A( ' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h & A(& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A((& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&", +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &+1 +150 h 1 M&% h % A(*& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&/& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(2'X +100 (0% h %S M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%7  + A(6 +112 h  P%,% h % .t\PS.seek 0.499ml P%$' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%3  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%<  +37 h .t\CBV.dial AIV C'9  +38 h .t\PRV.dial between CBV and air P%?  +19 h  .t\PRV.dial :air P%D  +18 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +251 h ' .t\PRV.dial :waste2 P%) @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&J1 +2 h 1 M&;& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&M(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&:/ +150 h / M&E& h &- M&.% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'>& h &W .tHand.open M&I1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  C'%& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Z @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%8' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%B' +1094 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 0ml P%^' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%W +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%a  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %f'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%V  +76 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%i' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%e  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Y'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%l  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%m  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %o' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%b +75 h .t\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .t\PS.seek 0ml P%4' +2610 h ' .t\PSV.dial :air P%r  +75 h  .t\PS.seek 2.5ml P%q' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%k +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%n' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%v' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%w +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%y  +75 h P%{% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %j' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'd & +50 h & .t\AWV.select :waste C' +7 P%|% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %`'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'K { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%~' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'z& h &W F*] @@T .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR ."MFBPCR"\ASV.dial :SPEdelivery P% F*  +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*[% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%* +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%/' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial PSR P%' +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*,9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%_ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%<' +2609 h ' .t\PSV.dial PSR P%9 +113 h P%?% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%$' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %;'k +2679 h 'k .t\PSV.dial :waste1 P%1  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *"'V +849 h ' .t\PSV.dial :air P%:  +113 h  .t\PS.seek 2.5ml P%E' +2610 h ' .t\PSV.dial :mixloop P%. +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %5' +5106 h ' .t\PSV.dial :air P%N  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %&' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *Q'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*I' +171 h ' .t\PSV.dial :mixloop P%F +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +619 h ' ."MFBPCR"\ATV.dial :waste F*Z n +128 h n ."MFBPCR"\AS.seek :empty F*O'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*\\  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F*8% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'B  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'a'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'^  'V  +38 h  +113  .t\CBV.dial :waste1 C'! @ +38 h @ .t\CS.seek 0ml C'f'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*e  +22 h ."MFBPCR"delay 3 seconds +300 F*i% h %_ ."MFBPCR"\AS.seek 0.434ml F*l'ݏ +110 h '4 .t\CSV.dial CSR C'm  +38 h  C'Y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'b'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*c  +22 h F*o% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*r'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*4 n +107 h n F*k% h %& ."MFBPCR"\AS.seek 0.092ml F*q'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'w'4 +2647 h '4 C'y& h &W #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'`( +416 h ( M&L& h &ӷ M&T% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 4 into Collection Clamp C'x& h & M&h% h %\  A(% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (X(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (}& h &,9 M&& h &- M&\ % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A([& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A(' +76 h ' A(s& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M& (w +7 h (w M&(& h &w A(& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&+'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h &w A(*'X M&% h %7 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' ."WCR"\CC.seek :closed C''(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +370 h (W ."WCR"Hand.open M& 1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *2' +29 h '\  ."WCR"\SC.coast #collectSample 0ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)_% h % C'7& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'3  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +75 h @ +50 S)F% h % ."WCR"\CBV.dial between CSV and waste2b C'Q  +94 h  C'&% h % ."WCR"\CSV.dial between air and CTV C'Z ` +19 h ` ."WCR"\CS.seek 7.288ml C'I' +164 h ' ."MFBPCR"delay 5 seconds +317 h ' ."connect"\CTV.dial CSV,avoiding: :air C'\\  ."WCR"\CSV.dial CTV C'S  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'O'4 +107 ."MFBPCR"\ATV.dial :air2 F*6  +65 h  F*8% h % ."MFBPCR"\AS.seek 0.52ml F*P' +1747 h '4 ."WCR"\CBV.dial :waste2b C'B  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'W' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'g  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'a  +38 h  C'^% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'V' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)e% h % ."WCR"\CBV.dial between CSV and waste2b C'i  +22 h ' ."MFBPCR"delay 5 seconds +72 h  C'm% h % ."WCR"\CSV.dial between air and CTV C'Y ` +57 h ` ."WCR"\CS.seek 7.288ml C't' +371 ."MFBPCR"\ATV.dial ASV F*f n +64 h n ."MFBPCR"\ASV.dial :wasteR F*c  +21 h  ."MFBPCR"\AS.seek 0.3ml F*o': +25 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'l  '4  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'k'4 +1919 h '4 ."WCR"delay 2:00 +2351 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*r'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*b % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F*y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *{'N +439 ."WCR"\CBV.dial :waste2b C'd  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '|' +41 h ' ."WCR"delay 1:00 +528 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*K I +43 h I F*G% h %N ."MFBPCR"\AS.seek 0.109ml F*R'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*C n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*L  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *T% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *j' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +79 ."WCR"\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'z  +38 h  C'v% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C' ' +27 ."MFBPCR"\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *' +574 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'x' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'h @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'p  +94 h  C'}% h % ."WCR"\CSV.dial between air and CTV C' ` +56 h ` ."WCR"\CS.seek 7.288ml C'\ ' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '['4 +1919 h '4 ."WCR"delay 2:00 +1031 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*s% h % ."MFBPCR"\AS.seek 0.279ml F*'Q +933 ."WCR"\CBV.dial :waste2b C'(  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'~  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&+% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A(*& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +89 h ' A(& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(`'K +253 h 'K M&q& h & A( & h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &' +26 h ' ."WCR"\Elbow.hold M& , +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(_- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(7' +27 h ' M&3% h % A(/& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &0'B +20 h 'B M&X& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &?' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(9' +37 h ' M&<& h & M&A% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &D'\ +89 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&$& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ ."WCR"\Forearm.seek CC M&J' +91 h ' ."WCR"Hand.close M&2/ +150 h / ."WCR"\CC.seek :open C'1( +415 h ( A()& h & A(@& h & M&H& h &ӷ M&:% h % ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &.( +143 h ( M&;& h & A(5& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &U' +92 h ' ."WCR"\Elbow.hold M&M, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &E1 +150 h 1 M&"% h % A(w& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&>'B +20 h 'B M&F& h & A(Q& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &&' +22 h ' A(Z% h % ."downElevator"\SE.seek :down A('X +100 (S% h %B M&\\% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &6'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'8& h & .tHand.open M&I1 +2 A(6 h 1 M&=& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&O(- +166 h (- M&g& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&W/ +150 h / M&V& h &- M&% h %η .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +264 h (ӷ M&m& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &!' +91 h ' .t\CC.seek :closed C'f(W +416 h (W C'c& h &W .tHand.open M&41 +150 h 1 M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C't& h &W C'o% h % .t\CSV.dial CSR C'b  +38 h  C'r% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'n'[ +463 h '[ .t\CSV.dial :waste C'd +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'k'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'K& h &W C'G% h %4 .t\CSV.dial :flush C'{  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'C'p +384 h 'Q ."MFBPCR"delay 30 seconds +266 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'L @ +38 h @ C'T% h %p .t\CS.seek 7ml C'R'i +415 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'z  +38 h  +113  .t\CBV.dial :waste1 C'v @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'|& h &W C'j% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +63 ."MFBPCR"\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *p'R +286 .t\CSV.dial :air C'} @ +38 h @ C'% h %p .t\CS.seek 7ml C'x'i +415 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'\ '4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +16 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +22 h  C's% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +15 h % F*(% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *['v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*~ I +21 h I F*% h %v ."MFBPCR"\AS.seek 0.059ml F*'H +454 h 'P .tdelay 5 seconds +171 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F*' +222 h ' ."MFBPCR"delay 2 seconds +86 .t\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +107 ."MFBPCR"\ATV.dial :bleach F*+  +22 h F*% h % ."MFBPCR"\AS.seek 0.769ml F**' q +580 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C','4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'q  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '_'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CTV C'/  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'X  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'<  +37 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C'` .t\CBV.dial CSV C'  +37 h +75 .t\CTV.dial :air C'$  +151 h .t\CS.seek 0ml C'u'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'0'0 +506 h '0 .t\CSV.dial :waste C'7 +38 h .t\CS.seek 0ml C'?'4 +507 h '4 #t\Flushing upper manifold C' & h &W C'2% h %4 .t\CSV.dial :flush C'D  +37 h  .t\CS.seek 5ml C'''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'@% h %p .t\CS.seek 7ml C'H'i +416 h 'i ."connect"\CSV.dial CBV C': .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C';'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +507 h '0 .t\CSV.dial :waste C'J +37 h .t\CS.seek 0ml C''4 +33 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +152 h '4 C'"& h &W C'w% h %4 .t\CSV.dial :flush C'F  +37 h  .t\CS.seek 3ml C'Q'0 +111 F*Z% h % q ."MFBPCR"\AS.seek 0.794ml F*#' k +396 h '0 .tdelay 5 seconds +129 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*S% h % k ."MFBPCR"\AS.seek 0.779ml F*\\'  +49 .t\CSV.dial :air C'8 @ +38 h @ C'I% h %0 .t\CS.seek 5ml C'='p +237 h '  ."MFBPCR"delay 3 seconds +179 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +121 ."MFBPCR"\ATV.dial :AIV F*U I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *>' +422 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'& +76 h .t\CS.seek 0ml C'g'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'M'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'V% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'6  .t\CTV.dial CSV C'^  +37 h  +113  .t\CBV.dial :waste1 C'm @ +38 h @ .t\CS.seek 0ml C'Y'4 +376 h ' ."MFBPCR"\ATV.dial :waste F*O n +129 h n ."MFBPCR"\AS.seek :empty F*e'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*1 % +43 h % F*c% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *4' +102 h '4 #t\Flushing lower manifold's waste2b port C'l& h &W C't% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C'b'p +45 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*!% h % ."MFBPCR"\AS.seek 0.579ml F*' +384 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +37 h @ C' % h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  +38 h  .t\CBV.dial :waste2b C'K  +75 h  .t\CS.seek 0ml C'G'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +7 h (ӷ M&L& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.seek CC M&C' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'z( +415 h ( M&v& h &ӷ M&R% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &j(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&}% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'h& h & .tHand.open M&1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &x(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&(/ +150 h / M&\ & h &- M&p% h %η .t\Forearm.seek :retract M&~'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'+& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h %4 .t\CSV.dial CSR C'  +38 h  C'q% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 ',% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +463 h '[ .t\CSV.dial :waste C'/ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'X'4 +2010 h '4 #t\Purging external Intake C'3& h &W C'<% h %4 .t\CSV.dial :flush C'A  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  ' +38 h +38 .t\CTV.dial :intake C'`  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'$'4 +415 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +173 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'u  +76 h #t\Flushing upper manifold C'7& h &W C'0% h %4 .t\CSV.dial :flush C'  +45 F*2% h %  ."MFBPCR"\AS.seek 0.604ml F*D'\ 48 +30 h  .t\CS.seek 5ml C'?'p +496 h '\ 48 ."MFBPCR"delay 3 seconds +154 h 'p .tdelay 5 seconds +146 ."MFBPCR"\ATV.dial :bleach F*)  +21 h F*@% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*'' +324 h '  ."MFBPCR"delay 3 seconds +9 .t\CSV.dial :air C': @ +38 h @ C'H% h %p .t\CS.seek 7ml C';'i +253 ."MFBPCR"\ATV.dial :ASV F*J n +86 h n ."MFBPCR"\ASV.dial :wasteR F*5  +43 h  ."MFBPCR"\AS.seek 0ml F*E'V +34 h 'i ."connect"\CSV.dial CBV C'" .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'w'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +506 h '0 .t\CSV.dial :waste C'Z +38 h .t\CS.seek 0ml C''4 +506 h '4 C'*& h &W C'.% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.seek 3ml C'8'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'I @ +37 h @ C'#% h %0 .t\CS.seek 5ml C'Q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'U'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'='0 +507 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'B'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &9(ӷ +6 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&g/ +150 h / .t\CC.seek :open C'W( +416 h ( M&V& h &ӷ M&M% h % .t\Forearm.seek :retract M&6'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &^(- +265 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&11 +150 h 1 M&c% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +90 h '\  #t\Completed [postDeployPhase] begun 01:00:31 #\Extending 18tun_postDeploy until completion of MFBPCR +3920 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *>'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*o % +108 h % F*e% h %V ."MFBPCR"\AS.seek 0.59ml F*r' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*! n +108 h n ."MFBPCR"\ASV.dial :wasteR F*Y% h %  ."MFBPCR"\AS.seek 0.09ml F*4' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*b% h % ."MFBPCR"\AS.seek 0.12ml F*n' +625 h ' ."MFBPCR"\ATV.dial :air3 F*K % +21 h % F*d% h % ."MFBPCR"\AS.seek 0.13ml F*{' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*T% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*i % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h % ."MFBPCR"\AS.seek 0.255ml F*f'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*R% h %X ."MFBPCR"\AS.seek 0.24ml F*C' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *}'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*| @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*s  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *('\  +1032 h '\  F*\ % h %\  ."MFBPCR"\AS.seek 0.5ml F*p'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*j% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1031 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*['(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *x' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*~ % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +3472 h 'V ."MFBPCR"\SPE.coast F*+  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*q n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*,  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *_'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*3  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*<% h %Fj ."MFBPCR"\AS.seek 0.524ml F*A' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*X  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*` % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*u  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*2 n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*)% h %\  ."MFBPCR"\AS.seek 0.65ml F*@' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 F*:  +65 h ."MFBPCR"\ARV.dial :air1 F*H  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*" % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refiH; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*'% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*w  +22 h ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +21 h F*S% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*8'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*U% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*&  +65 h  F*a% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*g' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*e% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*!  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0104h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ARV.dial :carrier F*i  +11 h  F*P% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *f'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *k'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*}  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*h' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*j  +108 h  ."MFBPCR"delay 7 seconds +700 F*p% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*['\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*~  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.65ml F*+' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*z'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*q % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0104h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *G'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*u  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*7  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *2'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +31 h  ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*"% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +21 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*;'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*.  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*g% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *V'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *1'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*c n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*9% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*E I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.53ml F*o' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*e % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*!'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +108 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*r'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*y n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.65ml F*d' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*n  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*L  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *C'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +21 h F*h% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*j% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *p'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*[  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*$ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *)'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*H  +22 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *D'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*5 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*' n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Z  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*.  +108 h  ."MFBPCR"delay 7 seconds +700 F*;% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +21 h n ."MFBPCR"delay 2 seconds +200 F*I% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*=  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*&% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*g % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h.pcr F*a% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *m'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*6% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*t  +64 h  F*o% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Y' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +32 h  ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*n% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*d% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*f% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*R  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0111h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*p% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*[  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *x'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*+ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*| % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*,  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*< n +22 h n ."MFBPCR"delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.6ml F*X'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.65ml F*`' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*A  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*u% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0111h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*7 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*0  +151 h F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *:' +423 h ' ."MFBPCR"\ATV.dial :ASV F*H n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*?  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*5'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*" n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *''V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*J'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*@ % +43 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +423 h ' ."MFBPCR"\ATV.dial :air3 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*.' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*w% h % ."MFBPCR"\AS.seek 0.71ml F**' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*#% h % Ű ."MFBPCR"\AS.seek 0.72ml F*8' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*= n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*&% h % zG ."MFBPCR"\AS.seek 0.365ml F*U' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *g'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*a % +43 h % F*\\% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*V% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %  ."MFBPCR"\AS.seek 0.604ml F*m'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*9% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*E n +86 h n ."MFBPCR"\ASV.dial :wasteR F*6  +22 h  ."MFBPCR"\AS.seek 0ml F*1'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*o % +108 h % F*O% h %V ."MFBPCR"\AS.seek 0.01ml F*>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*!% h % ."MFBPCR"\AS.seek 0.58ml F*y' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h % q ."MFBPCR"\AS.seek 0.605ml F*K'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*n% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*4' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*{ n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*d  +21 h  ."MFBPCR"\AS.seek 0ml F*L'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0ml F*'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*P  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *v' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*f n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *i'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*R n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*C n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +42 h  F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *}' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\  +43 h  F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*p  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *('Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*[ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*~  +43 h  F*+% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*x  +64 h  F*|% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*,% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*/  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *z': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*< n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *G'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*3 n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*_  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*X % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *A' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*`% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*)% h % ."MFBPCR"\AS.seek 0.97ml F*$'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *"'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *D'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*'  +43 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Z' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *J' +9988 h ' ."MFBPCR"\ATV.dial AIV F*] I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F*; n +129 h n ."MFBPCR"\AS.seek 0ml F*w'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*.% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*S  +22 h ."MFBPCR"\ATV.dial :air3 F** % +43 h % F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*=% h %Z ."MFBPCR"\AS.seek 0.81ml F*&'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*U'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*\\% h %n ."MFBPCR"\AS.seek 0.82ml F*g'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*M' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*6  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*1% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *e' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*o  +21 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*t'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*>'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*r% h %m ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*b  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *i'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*C'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0115h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*k  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*p  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*(' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*[  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*~ % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*x  +108 h  ."MFBPCR"delay 7 seconds +700 F*|% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*<  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*z% h % \\ ."MFBPCR"\AS.seek 0.326ml F*3'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*_ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0115h.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*u % +87 h % F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *A'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +21 h F*$% h %n ."MFBPCR"\AS.seek 0.82ml F*?'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *5'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*;  +31 h  ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F**% h % ."MFBPCR"\AS.seek 0.81ml F*Q'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*='m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*&  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *a'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *c'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0117h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*9  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*6% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*E n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*r% h %Fj ."MFBPCR"\AS.seek 0.524ml F*>' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*b  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*K  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*d'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*4  +108 h  ."MFBPCR"delay 7 seconds +700 F*L% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*P I +107 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*R' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*i  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*k'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0117h.pcr F*s% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*}  +21 h ."MFBPCR"\ATV.dial :air3 F*\ % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *p'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*j% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*(% h %n ."MFBPCR"\AS.seek 0.82ml F*~'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*+  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*x% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *|'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*/  +22 h F*G% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*7 % +22 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*3'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*u% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0119h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*?  +10 h  F*5% h %V ."MFBPCR"\AS.seek 0.5ml F*''(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Z'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*;  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*S n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F**% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*I I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*Q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*# % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*&  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*\\ I +108 h I ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*M  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*6 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0119h.pcr F*c% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*E  +22 h ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*t'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*{  +65 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*f % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*R% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*k'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +21 h F*\ % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*h  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0121h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +10 h  F*|% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*, n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*/ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*G% h %Fj ."MFBPCR"\AS.seek 0.524ml F*z' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*7 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*X% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*u% h % ."MFBPCR"\AS.seek 0.536ml F*3'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*`% h %< ."MFBPCR"\AS.seek 0.594ml F*2'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*)  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*"'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*5 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0121h.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*D  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*8  +64 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *&'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' @CET82808.26 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*c% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*1% h %m ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0200h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*n  +43 h  ."MFBPCR"\ARV.dial :carrier F*>  +11 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F*L'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*i' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*C % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*}  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*k'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*h  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*j I +108 h I ."MFBPCR"delay 2 seconds +200 F*p% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*(  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*+% h % \\ ."MFBPCR"\AS.seek 0.326ml F*x'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*~  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0200h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*q'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*z'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*u  +65 h  F*_% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*0 % +87 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +21 h % ."MFBPCR"delay 3 seconds +300 F*:% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +21 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #t\Asleep after completing 18tun_postDeploy assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:54:37, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy assays" #\Mission runtime was 2:39.834632 #-> ls !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message ls !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message exit #m\EXIT @1542643018.61CET @1542643026.55CET = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiP!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:41:46CET02-Jan-70 .delayUntil "02:41:46.00CET02-Jan-70" @CET92506.00 #-> exit #m\EXIT @1542643037.23CET @1542643358.96CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:02:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: postDeployPhase\\n/home/esp/esp2/mission/18tun_postDeploy.rb:104\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:03:08, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:03:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy phase postDeployPhase" #\Phase [postDeployPhase] #==================> STARTED postDeploycoreNegNTC (phase #1) <=== #\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 4 at 17:03:12CET19-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:03:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil M&11 F* +6 *2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'T& h &W ."airStroke"\CSV.dial :waste S)U% h % ."airStroke"\CS.seek 0ml E ;3\\0\\0 C'V'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9d +100 C'M9 h 9 d +100 C'N9 h 9$d +100 C'F9 h 9(d +100 C'@9 h 9 ,d +100 C'I9 h 9$0d +100 C'C9 h 9<4d +100 C'K9 h 9S8d +100 C'.9 h 9kd +100 C'e9 h 9Td +100 C'f9 h 9kd +100 C'g9 h 9d +100 C'h9 h 9d +100 C'i9 h 9d +100 C'_9 h 9d +100 C'Y9 h 9d +100 C'k9 h 9d +100 C'l9 h 9 d +100 C'm9 h 9 d +100 C'd9 h 96d +100 C'o9 h 9Ld +100 C'`9 h 9bd +100 C'q9 h 9xd +100 C'r9 h 9d +100 C's9 h 9d +100 C'n9 h 9d +100 C't9 h 9d +100 C'v9 h 9d +100 C'w9 h 9d +100 C'y9 h 9d +100 C'u9 h 9(d +100 C'{9 h 9>d +100 C'j9 h 9Td +100 C'z9 h 9id +100 C'x9 h 9d +100 C'|9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C' 9 h 9 $d +100 C'\ 9 h 9 (d +100 C' 9 h 9 ,d +100 C' 9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9   +18 h C'M% h %4 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'F  +37 h C'@% h %p .t\CS.seek 7ml C'I'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'C @ +37 h @ .t\CS.seek 10ml C'K' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'. ` +132 h ` #tLysing Complete C'Q& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&R% h %\  A(T% h %X A(U. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (O& h & M&8& ."raiseStack"\SE.seek :upperLimit h &ӷ A( 'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C']( +23 h 'Z +393 ( A(^& h & A(W& h & M&4& h &ӷ M&a% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +142 h ( M&e& h & A(f& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &g' +93 h ' .t\Elbow.hold M&h, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&_% h % A(Y& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&k'B +20 h 'B M&l& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(o% h %Z ."downElevator"\SE.seek :down A(`'X +100 (q% h % M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'n @ +2 A(6 +17 h @ .t\CS.seek 10ml C't' +6 h ' .t\CSV.dial between air and CTV C'v ` +19 h ` .t\AIV.dial :bypass C'w { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'y& h & .tHand.open M&u1 +2 h 1 M&{& h & M&j% h %\  .t\Elbow.seek FlushPuck::Garage M&z(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&}'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&\ 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +112 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.seek 2.5ml P%+' +2610 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +6 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'%( +416 h ( M&2& h &ӷ M&0% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&$1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'<& h & M&9% h %\  A(?% h %X A(D. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (B(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &,9 M&=& h &- M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&H& h &w A(J& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(;& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(>' +66 h ' A(M& h &,9 M&#& h &w .t\Elbow.seek 4 M&F(w +6 h (w M&@& h &w A(N& h &,9 .t\Forearm.seek 4 + 300 counts M&C'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down A(I'X M&.& h &w M&Q% h %7 .t\Forearm.seek :retract M&R'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&T(ӷ +143 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'[(W +416 h (W .tHand.open M&Z1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'^'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'W' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'4'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%a  +37 h  .t\PS.seek 2.5ml P%e' +2610 h ' .t\PSV.dial :mixloop P%f +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%V  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%i  +151 h  .t\PS.seek :empty P%_' +2609 h ' .t\CBV.dial :waste1 C'Y @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'l  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'm @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'o  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'b ` +19 h ` .t\CSV.dial :air C'c @ +37 h @ .t\CS.seek 10ml C'q' +2647 h ' .t\CSV.dial CTV C'r  +38 h  .t\CS.seek 0ml C'g'4 +2647 h '4 .t\CBV.dial :waste1 C'k @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'n  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&v% h %\  A(w% h %X A(y. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (u( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ({& h & M&j& h &ӷ M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &x( +143 h ( M&|& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(K& h & .t\SE.seek -0.29 Pucks below Top Plate A(h'\\ +264 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&s, +6 h , .t\Elbow.reconfigure TightElbow A(~- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&% h % A(z& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &}'B +20 h 'B M&]& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A( ' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h & A(& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A((& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&", +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &+1 +150 h 1 M&% h % A(*& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&/& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(2'X +100 (0% h %S M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%7  + A(6 +112 h  P%,% h % .t\PS.seek 0.499ml P%$' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%3  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%<  +37 h .t\CBV.dial AIV C'9  +38 h .t\PRV.dial between CBV and air P%?  +19 h  .t\PRV.dial :air P%D  +18 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +251 h ' .t\PRV.dial :waste2 P%) @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'H& h & .tHand.open M&J1 +2 h 1 M&;& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&M(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&:/ +150 h / M&E& h &- M&.% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'>& h &W .tHand.open M&I1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  C'%& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Z @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%8' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%B' +1094 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 0ml P%^' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%W +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%a  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %f'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%V  +76 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%i' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%e  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Y'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%l  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%m  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %o' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%b +75 h .t\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .t\PS.seek 0ml P%4' +2610 h ' .t\PSV.dial :air P%r  +75 h  .t\PS.seek 2.5ml P%q' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%k +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%n' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%v' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%w +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%y  +75 h P%{% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %j' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'd & +50 h & .t\AWV.select :waste C' +7 P%|% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %`'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'K { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%~' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'z& h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*] @@T .t\AIV.dial :bypass F*  .t\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*[% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%* +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%/' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial PSR P%' +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*,9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%_ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%<' +2609 h ' .t\PSV.dial PSR P%9 +113 h P%?% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%$' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %;'k +2679 h 'k .t\PSV.dial :waste1 P%1  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *"'V +849 h ' .t\PSV.dial :air P%:  +113 h  .t\PS.seek 2.5ml P%E' +2610 h ' .t\PSV.dial :mixloop P%. +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %5' +5106 h ' .t\PSV.dial :air P%N  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %&' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *Q'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*I' +171 h ' .t\PSV.dial :mixloop P%F +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +619 h ' ."MFBPCR"\ATV.dial :waste F*Z n +128 h n ."MFBPCR"\AS.seek :empty F*O'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*\\  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F*8% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'B  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'a'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV C'^  .t\CTV.dial CSV C'V  +38 h  +113  .t\CBV.dial :waste1 C'! @ +38 h @ .t\CS.seek 0ml C'f'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*e  +22 h ."MFBPCR"delay 3 seconds +300 F*i% h %_ ."MFBPCR"\AS.seek 0.434ml F*l'ݏ +110 h '4 .t\CSV.dial CSR C'm  +38 h  C'Y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'b'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*c  +22 h F*o% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*r'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*4 n +107 h n F*k% h %& ."MFBPCR"\AS.seek 0.092ml F*q'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'w'4 +2647 h '4 C'y& h &W #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'`( +416 h ( M&L& h &ӷ M&T% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 4 into Collection Clamp C'x& h & M&h% h %\  A(% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (X(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (}& h &,9 M&& h &- M&\ % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A([& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A(' +76 h ' A(s& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M& (w +7 h (w M&(& h &w A(& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&+'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(*'X h &w M&% h %7 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' ."WCR"\CC.seek :closed C''(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +370 h (W ."WCR"Hand.open M& 1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *2' +29 h '\  ."WCR"\SC.coast #collectSample 0ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)_% h % C'7& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'3  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +94 h  C'F% h % ."WCR"\CSV.dial between air and CTV C'Q ` +19 h ` ."WCR"\CS.seek 7.288ml C'&' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'Z  'I  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '\\'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'S  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'O' +41 h ' ."WCR"delay 1:00 +5837 h ' ."MFBPCR"delay 5 seconds +163 ."WCR"\CTV.dial :air,avoiding: :intake C'6  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'8  +38 h  C'P% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +143 ."MFBPCR"\ATV.dial ASV F*W n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*g' +399 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'a' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'^ @ +75 h @ +50 S)V% h % ."WCR"\CBV.dial between CSV and waste2b C'!  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'e ` +57 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'm  'Y  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 't'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'f  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'c' +41 h ' ."WCR"delay 1:00 +3122 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*o  +65 h  F*l% h % ."MFBPCR"\AS.seek 0.52ml F*4' +2313 ."WCR"\CTV.dial :air,avoiding: :intake C'k  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'r  +37 h  C'b% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'n' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'd  +94 h  C'|% h % ."WCR"\CSV.dial between air and CTV C'K ` +56 h ` ."WCR"\CS.seek 7.288ml C'G' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'R  'C  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'L'4 +1919 h '4 ."WCR"delay 2:00 +2814 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*T n +64 h n ."MFBPCR"\ASV.dial :wasteR F*j  +21 h  ."MFBPCR"\AS.seek 0.3ml F*': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*z'w +2155 ."WCR"\CBV.dial :waste2b C'v  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +2432 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*x  +87 h F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *'N +1005 ."WCR"\CTV.dial :air,avoiding: :intake C'p  +22 h 'N ."MFBPCR"delay 3 seconds +129 h C'}& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(\ % h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +171 ."MFBPCR"\ATV.dial :ElutionWater F*[ I +29 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&s% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +14 h I F*(% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +129 h ( M&~& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A('K +253 h 'K M&& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' ."WCR"\Elbow.hold M&+, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&*% h % A( & h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&`& h & A(q& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M& & h & M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +89 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +143 h (ӷ M&/& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ ."WCR"\Forearm.seek CC M&X' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'?( +144 h '{ ."MFBPCR"delay 3 seconds +272 h ( A(9& h & A(<& h & M&A& h &ӷ M&D% h % ."WCR"\Forearm.seek :retract M&'\ +28 ."MFBPCR"\ATV.dial ASV,via: :air2 F*$ n +63 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &u( +109 h n ."MFBPCR"\ASV.dial :SPEelution F*J  +33 h ( M&)& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &;' +10 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *@% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *H' ?x +76 h ' ."WCR"\Elbow.hold M&:, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &=1 +150 h 1 M&E% h % A(N& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&5'B +20 h 'B M&#& h & A(U& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &w' +22 h ' A("% h % ."downElevator"\SE.seek :down A('X +100 (>% h %B M&F% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&Z1 +2 A(6 h 1 M&I& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&S(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.seek :garage M&8'η +91 h 'η .tHand.close M&P/ +150 h / M&W& h &- M&O% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +265 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &!' +91 h ' .t\CC.seek :closed C'(W +415 h (W C'e& h &W .tHand.open M&a1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'f% h % .t\CSV.dial CSR C't  +38 h  C'o% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1227 h ' ?x ."MFBPCR"delay 3 seconds +146 h 'p .tdelay 5 seconds +154 ."MFBPCR"\AFV.select :holdingCoil F* +6 *g% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *k' +340 .t\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'b'[ +463 h '[ .t\CSV.dial :waste C'c +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'y& h &W C'{% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C' % h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'C  'G  +37 h  +113  .t\CBV.dial :waste1 C'T @ +75 h @ .t\CS.seek 0ml C'j'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'4& h &W C'% h %4 .t\CSV.dial :flush C'v  +76 h  .t\CS.seek 5ml C'L'p +76 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *z' +74 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'x @ +38 h @ C'h% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'}  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'\  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +470 h ' ."MFBPCR"delay 1:00 +30 .t\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C's  +38 h  .t\CBV.dial :waste1 C'[ @ +75 h @ .t\CS.seek 0ml C'('4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'~  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '*'P +788 #"MFBPCR"pushing eluate to holding coil F* % h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +328 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'_  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'/'4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +37 h @ C'% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'D'4 +507 h '4 C'$& h &W C')% h %4 .t\CSV.dial :flush C'1  +37 h  .t\CS.seek 3ml C'J'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'3'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'='4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +506 h '0 .t\CSV.dial :waste C'N +76 h .t\CS.seek 0ml C'#'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'U& h &W C'"% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'>% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV C'M  .t\CTV.dial CSV C'Z  +38 h  +112  .t\CBV.dial :waste1 C'I @ +38 h @ .t\CS.seek 0ml C'&'4 +766 h '4 #t\Flushing lower manifold's waste2b port C';& h &W C'5% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +651 h 'p .tdelay 5 seconds +108 h 'Q ."MFBPCR"delay 30 seconds +392 .t\CSV.dial :air C'\\ @ +37 h @ C'6% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C':  +75 h  .t\CS.seek 0ml C'P'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'S( +416 h ( M&2& h &ӷ M&?% h % .t\Forearm.seek :retract M&e'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &a(- +265 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &f'η +91 h 'η .tHand.open M&t1 +150 h 1 M&o% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +16 ."MFBPCR"\ATV.dial :waste,via: :air3 F* n +75 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&H1 +2 h 1 M&r& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &l(- +6 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&y'η +68 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +23 h 'η .tHand.close M&d/ +150 h / M&n& h &- M&K% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +265 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &T' +90 h ' .t\CC.seek :closed C'R(W +416 h (W C'4& h &W .tHand.open M&1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'k% h %4 .t\CSV.dial CSR C'x  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'L'p +314 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*p % +43 h % F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*\ I +21 h I F* % h %v ."MFBPCR"\AS.seek 0.059ml F*'H +369 h 'p .tdelay 5 seconds +256 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*s% h %H ."MFBPCR"\AS.seek 0.069ml F*[' +222 h ' ."MFBPCR"delay 2 seconds + .t\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '~'[ +193 ."MFBPCR"\ATV.dial :bleach F*  +22 h F*(% h % ."MFBPCR"\AS.seek 0.769ml F*' q +248 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'z  +38 h +38 .t\CTV.dial :intake C'*  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'_'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'q% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'0% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'/'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C' '4 +506 h '4 C''& h &W C'7% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C'A'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'$% h %0 .t\CS.seek 5ml C')'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +506 h '0 .t\CSV.dial :waste C'@ +75 h .t\CS.seek 0ml C'X'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&J& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&E' +90 h ' .tHand.close M&U/ +150 h / .t\CC.seek :open C'"( +416 h ( M&& h &ӷ M&#% h % .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +96 h ' q ."MFBPCR"delay 3 seconds +169 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &Z'η +91 h 'η .tHand.open M&I1 +40 ."MFBPCR"\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +88 h 1 M&;% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Completed [postDeployPhase] begun 01:00:31 #\Extending 18tun_postDeploy until completion of MFBPCR +121 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*&' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F*6% h % k ."MFBPCR"\AS.seek 0.779ml F*w'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *:' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*W'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*8% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*m% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %  ."MFBPCR"\AS.seek 0.604ml F*^'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*i% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*O' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +86 h n ."MFBPCR"\ASV.dial :wasteR F*o  +43 h  ."MFBPCR"\AS.seek 0ml F*e'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *H'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*r % +108 h % F*c% h %V ."MFBPCR"\AS.seek 0.59ml F*b' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*f% h %  ."MFBPCR"\AS.seek 0.09ml F*d' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*K I +86 h I F*a% h % ."MFBPCR"\AS.seek 0.12ml F*!' +625 h ' ."MFBPCR"\ATV.dial :air3 F*` % +21 h % F*y% h % ."MFBPCR"\AS.seek 0.13ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*l% h % ."MFBPCR"\AS.seek 0.23ml F* ' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*h  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *p'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*} @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*\ I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*(% h %(A ."MFBPCR"\AS.seek 0.6ml F*L'\  +1031 h '\  F*[% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*~% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*,  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*z  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*_  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*/' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*7 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +108 h  ."MFBPCR"delay 7 seconds +700 F*$% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +21 h n ."MFBPCR"delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*N  +43 h  ."MFBPCR"\ASV.dial :waste1 F*U  +65 h ."MFBPCR"\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*j% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*E% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*M  +22 h ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *I'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*g'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*c% h %m ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0104h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ARV.dial :carrier F*S  +11 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*k  +22 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*p  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*s' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*['\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*L n +22 h n ."MFBPCR"delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0104h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*_  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*9% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*<  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*U  +31 h  ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*#% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*M'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*I' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*8  +10 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *V'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *^'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*t n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*e I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*r' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*H  +108 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +21 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*C% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*p'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*s  +65 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*'  +11 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*1'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*|  +22 h ."MFBPCR"delay 2 seconds +200 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *N'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*=' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*E n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*M'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*;  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*I n +21 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h.pcr F*Q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F*B % +86 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*^'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*g  +64 h  F*r% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*a % +86 h % F*!% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0111h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*~ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *['R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +21 h ."MFBPCR"delay 2 seconds +200 F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*, I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*} % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*_  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*q  +22 h  ."MFBPCR"delay 7 seconds +700 F*0% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*+  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*7' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*9  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*/  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0111h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*$ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*1  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*A  +151 h F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *U' +423 h ' ."MFBPCR"\ATV.dial :ASV F*| n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*j  +43 h  F*@% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*# n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *E'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*R'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*= % +43 h % F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +423 h ' ."MFBPCR"\ATV.dial :air3 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*;' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*M% h % ."MFBPCR"\AS.seek 0.71ml F*I' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*\\' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*&' +7161 h ' ."MFBPCR"\ATV.dial :waste F*8 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*V% h % ."MFBPCR"\AS.seek 0.579ml F*W' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F*i% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*o  +22 h  ."MFBPCR"\AS.seek 0ml F*^'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *g'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*r % +108 h % F*O% h %V ."MFBPCR"\AS.seek 0.01ml F*H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*n' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h % q ."MFBPCR"\AS.seek 0.605ml F*`'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*!% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*d' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*C n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*G'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*4  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *v' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *S'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +202 h 'R ."MFBPCR"\SPE.coast F*P  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*x'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*k n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*\  +42 h  F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *p' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*~ n +65 h n F*L% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *[' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*}% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F** n +65 h n F*_% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*0  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*+'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*7% h % ."MFBPCR"\AS.seek 0.96ml F*''0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %0 ."MFBPCR"\AS.seek 0.985ml F* ' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*J% h % ."MFBPCR"\AS.seek 0.97ml F*D'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*| I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *#'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *N'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +43 h  F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *R' +9988 h ' ."MFBPCR"\ATV.dial AIV F*] I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*M'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*;% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*u  +22 h ."MFBPCR"\ATV.dial :air3 F*I % +43 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*:'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*B  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +64 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*^% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *c' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +21 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*g'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *S'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*h % +108 h % ."MFBPCR"\AS.seek 0ml F*k'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0115h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*\  +64 h  ."MFBPCR"\ARV.dial :carrier F*T  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*L% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.53ml F*z' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F** I +108 h I ."MFBPCR"delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0115h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*+  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b */'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +21 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*U  +65 h  F*#% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*; % +87 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *M' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\\% h %m ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0117h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*o% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*c  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*K  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*b% h %Fj ."MFBPCR"\AS.seek 0.524ml F*H' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*a  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*!' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*`  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.536ml F*y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*G% h %< ."MFBPCR"\AS.seek 0.594ml F*l'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*4 I +107 h I ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*h  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*k % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0117h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*p  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*~'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*z  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*q % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*7'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0119h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*E'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*;  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *R'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*u n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*M' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*Z I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*Q'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*o % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0119h.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*e  +22 h ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*P  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*x'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0121h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*_ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*0 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*$ n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*)'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*1 I +108 h I ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*A'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*|' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*#'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0121h.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*N  +22 h ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +64 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' @CET82808.26 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F*B % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*:% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*?  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0200h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*!  +43 h  ."MFBPCR"\ARV.dial :carrier F*H  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*\ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*S' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*k % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*x' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*( I +108 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0200h.pcr F*z% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*_  +21 h ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*+  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +31 h  ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +5060 h 'V #t\Asleep after completing 18tun_postDeploy assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:03:57, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy assays" #\Mission runtime was 2:39.088731 #-> exit #m\EXIT @1542643556.64CET @1542644060.37CET = =tquick =ECan =mquickScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refi; : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #Waiting for espclients... #"bkha"\Session Begun #-> showlog #-> abort! !o:CmdServer::Client::Error:bt[ "7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg":Attempt to Abort Mission without specifying a reason! #-> abort! "test" !o:CmdServer::Client::Error:bt[" bkha:3:in `topLevelBinding'" # simulerer lige #-> execute "18tun_postDeploy.rb" .GoodNews.suppressMail "Starting 18tun_postDeploy w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:16:08, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #"bkha"\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 ."bkha"GoodNews.suppressMail "Then, in tube 4: postDeployPhase\\n/home/esp/esp2/mission/18tun_postDeploy.rb:104\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:17:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" #"mwj"-> exit #\Session Ended +1499 E ;3\\0\\0 ."bkha"Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ ."bkha"\SPE.coast F*  h  ."bkha"\SPE.configure SPEconfig F*\ 2 *% h % ."bkha"\AS.configure ASconfig F*#,,'T(^20( b S)% h % ."bkha"\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:22:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy phase postDeployPhase" #\Phase [postDeployPhase] #==================> STARTED postDeploycoreNegNTC (phase #1) <=== #\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 4 at 17:22:22CET19-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:22:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*/% #"bkha"Loading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB #Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil ."bkha"Hand.open F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., ."bkha"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 ."bkha"\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 ."bkha"\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 ."bkha"\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w ."bkha"\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 ."bkha"\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 ."bkha"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&I& A(H'X h &w M&J% h %7 ."bkha"\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."bkha"\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' ."bkha"\CC.seek :closed C'P(W +415 h (W ."bkha"Hand.open M&Q1 +150 h 1 M&R% h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  ."bkha"\SC.coast #skipping prime and sample collection A(6 #"bkha"\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'T& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S)V% C'U'4 h % E ;3\\0\\0 #"bkha"\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9d +100 C'M9 h 9 d +100 C'N9 h 9$d +100 C'F9 h 9(d +100 C'@9 h 9 ,d +100 C'H9 h 9$0d +100 C'C9 h 9<4d +100 C'K9 h 9S8d +100 C'.9 h 9kd +100 C'e9 h 9Td +100 C'f9 h 9kd +100 C'g9 h 9d +100 C'h9 h 9d +100 C'i9 h 9d +100 C'_9 h 9d +100 C'Y9 h 9d +100 C'k9 h 9d +100 C'l9 h 9 d +100 C'm9 h 9 d +100 C'd9 h 96d +100 C'o9 h 9Ld +100 C'`9 h 9bd +100 C'q9 h 9xd +100 C'r9 h 9d +100 C's9 h 9d +100 C'n9 h 9d +100 C't9 h 9d +100 C'v9 h 9d +100 C'w9 h 9d +100 C'y9 h 9d +100 C'u9 h 9(d +100 C'{9 h 9>d +100 C'j9 h 9Td +100 C'z9 h 9id +100 C'x9 h 9d +100 C'|9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C' 9 h 9 $d +100 C'\ 9 h 9 (d +100 C' 9 h 9 ,d +100 C' 9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9   +18 h C'M% h %4 ."bkha"\CS.seek 5ml C'N'p +1373 h 'p ."bkha"delay 5 seconds +500 .\CTV.dial :air C'F  +37 h C'@% h %p ."bkha"\CS.seek 7ml C'H'i +607 h 'i ."bkha"delay 5 seconds +500 .\CSV.dial :air2 C'C @ +37 h @ ."bkha"\CS.seek 10ml C'K' +860 h ' ."bkha"\CSV.dial between air and CTV,avoiding: CBV C'. ` +132 h ` #"bkha"Lysing Complete C'Q& h &W #"bkha"\Storing sh1 puck in Collection Clamp to tube 3 M&R% h %\  A(T% h %X A(V. h . ."bkha"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +200 h ( ."bkha"\SC.reconfigure SCloose A($.(\ 1~b (O& h & M& & ."raiseStack"\SE.seek :upperLimit h &ӷ A(8'Z ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&Z& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ ."bkha"\Forearm.seek CC M&U' +91 h ' ."bkha"Hand.close M&\\/ +150 h / ."bkha"\CC.seek :open C']( +23 h 'Z +393 ( A(^& h & A(W& h & M&4& h &ӷ M&a% h % ."bkha"\Forearm.seek :retract M&b'\ +91 h '\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +142 h ( M&e& h & A(f& h & ."bkha"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &g' +93 h ' ."bkha"\Elbow.hold M&h, +6 h , ."bkha"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&_% h % A(Y& h & ."bkha"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."bkha"\Forearm.seek 3 + 300 counts +2 M&k'B +20 h 'B M&l& h & A(m& h & ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(o% h %Z ."downElevator"\SE.seek :down A(`'X +100 (q% h % M&r% h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 ."bkha"\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'n @ +2 A(6 +17 h @ ."bkha"\CS.seek 10ml C't' +6 h ' ."bkha"\CSV.dial between air and CTV C'v ` +19 h ` ."bkha"\AIV.dial :bypass C'w { +73 h { #"bkha"Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'y& h & ."bkha"Hand.open M&u1 +2 h 1 M&{& h & M&j% h %\  ."bkha"\Elbow.seek FlushPuck::Garage M&z(- +166 h (- M&x& h &- ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- ."bkha"\Forearm.seek :garage M&~'η +91 h 'η ."bkha"Hand.close M&/ +150 h / M&& h &- M&% h %η ."bkha"\Forearm.seek :retract M&}'\ +90 h '\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."bkha"\CC.seek :closed C'(W +416 h (W C' & h &W ."bkha"Hand.open M&\ 1 +150 h 1 M& % h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  ."bkha"\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #"bkha"\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 ."bkha"\PS.seek 1.5ml P%' +1597 h ' ."bkha"delay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  ."bkha"\PS.seek 0ml P%' +1598 h ' ."bkha"\PSV.dial PSR P% +37 h ."bkha"\PSR.select :air P% +7 ."bkha"\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV ."bkha"\CBV.dial PRV P%  C'  h +76 ."bkha"\PSV.dial :mixloop P% +113 h ."bkha"\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +112 h ."bkha"\PS.seek 0ml P%' +2610 h ' ."bkha"\PSV.dial :air P%  +38 h  ."bkha"\PS.seek 2.5ml P%' +2610 h ' ."bkha"\PSV.dial :mixloop P% +37 h ."bkha"\PS.seek 0ml P%' +2610 h ' ."bkha"\PSV.dial :air P%(  +37 h  ."bkha"\PS.seek 2.5ml P%,' +2610 h ' ."bkha"\PSV.dial :mixloop P%" +38 h ."bkha"\PS.seek 0ml P%' +2610 h ' #"bkha"Garaging FlushPuck in Collection Clamp M&& h &ӷ ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +6 h (ӷ M&*& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."bkha"\Forearm.seek CC M&'' +91 h ' ."bkha"Hand.close M&// +150 h / ."bkha"\CC.seek :open C'%( +416 h ( M&2& h &ӷ M&0% h % ."bkha"\Forearm.seek :retract M&3'\ +91 h '\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&5& h &- ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η ."bkha"Hand.open M&$1 +150 h 1 M&% h %η ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #"bkha"\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'<& h & M&9% h %\  A(?% h %X A(D. h . ."bkha"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (B(,9 +200 h (,9 ."bkha"\SC.reconfigure SCloose A($.(\ 1~b (A& h &,9 M&=& h &- M&)% h %\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&I& h &w A(J& h &,9 ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(;& h &,9 ."bkha"\SE.seek 19.71 Pucks below Top Plate A(>' +66 h ' A(M& h &,9 M&#& h &w ."bkha"\Elbow.seek 4 M&F(w +6 h (w M&@& h &w A(N& h &,9 ."bkha"\Forearm.seek 4 + 300 counts M&C'7 +22 h '7 ."bkha"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down M&H& A(.'X h &w M&Q% h %7 ."bkha"\Forearm.seek :retract M&R'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  ."bkha"\Elbow.seek CC M&T(ӷ +143 h (ӷ M&V& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' ."bkha"\CC.seek :closed C'Z(W +416 h (W ."bkha"Hand.open M&[1 +150 h 1 M&\\% h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  ."bkha"\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +150 h  ."bkha"\CSV.dial CTV C'P  +19 h  ."bkha"\CS.seek 5ml C'^'p +1372 h 'p ."bkha"delay 1:20 +8000 .\CS.seek 2.5ml C'W' +735 h '  ."bkha"delay 40 seconds +4000 .\CS.seek 0ml C'4'4 +735 h '4 ."bkha"delay 1:00 +6000 .\PSV.dial :air P%a  +37 h  ."bkha"\PS.seek 2.5ml P%e' +2610 h ' ."bkha"\PSV.dial :mixloop P%f +38 h ."bkha"delay 3 seconds +300 .\PSV.dial :air P%U  +37 h  ."bkha"delay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h ."bkha"delay 3 seconds +300 .\PSV.dial :waste1 P%i  +151 h  ."bkha"\PS.seek :empty P%_' +2609 h ' ."bkha"\CBV.dial :waste1 C'Y @ +38 h @ ."bkha"delay 5 seconds +500 .\CBV.dial AIV C'l  +38 h ."bkha"delay 10 seconds +1000 .\CBV.dial :waste1 C'm @ +37 h @ ."bkha"delay 10 seconds +1000 .\CBV.dial AIV C'o  +38 h ."bkha"delay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'b ` +19 h ` ."bkha"\CSV.dial :air C'c @ +37 h @ ."bkha"\CS.seek 10ml C'q' +2647 h ' ."bkha"\CSV.dial CTV C'r  +38 h  ."bkha"\CS.seek 0ml C'g'4 +2647 h '4 ."bkha"\CBV.dial :waste1 C'k @ +19 h @ ."bkha"delay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'n  +151 h C'8& h &W #"bkha"\Storing lyfil puck in Collection Clamp to tube 3 M&v% h %\  A(w% h %X A(y. h . ."bkha"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (u( +200 h ( ."bkha"\SC.reconfigure SCloose A($.(\ 1~b ({& h & M&j& h &ӷ M&d% h %\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &x( +143 h ( M&|& h & A(`& h & ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(K& h & ."bkha"\SE.seek -0.29 Pucks below Top Plate A(h'\\ +264 h '\\ M&& h & A(& h & ."bkha"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."bkha"\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' ."bkha"\Elbow.hold M&s, +6 h , ."bkha"\Elbow.reconfigure TightElbow A(~- M&$&.,(\ #b h -+\\ ."bkha"\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&% h % A(z& h & ."bkha"\Forearm.force -20 M&5 ."bkha"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &}'B +20 h 'B M&]& h & A( & h & ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' ."bkha"\SE.seek 1.04 Pucks below Top Plate A( ' +37 h ' M&& h & M&% h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."bkha"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."bkha"\Forearm.seek CC M&' +90 h ' ."bkha"Hand.close M&/ +150 h / ."bkha"\CC.seek :open C'( +416 h ( A( & h & A(& h & M&& h &ӷ M&% h % ."bkha"\Forearm.seek :retract M&'\ +91 h '\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A((& h & ."bkha"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' ."bkha"\Elbow.hold M&", +7 h , ."bkha"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &,1 +150 h 1 M&% h % A(*& h & ."bkha"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."bkha"\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&/& h & A(& h & ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(2'X +100 (0% h %S M&'% h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 ."bkha"\SC.coast .\PSV.dial :air P%7  + A(6 +112 h  P%+% h % ."bkha"\PS.seek 0.499ml P%$' +584 h ' ."bkha"delay 3 seconds +300 .\PSV.dial :mixloop P% +38 h ."bkha"\PRV.dial :air P%3  +38 h  ."bkha"delay 3 seconds +300 .\PRV.dial CBV P%<  +37 h ."bkha"\CBV.dial AIV C'9  +38 h ."bkha"\PRV.dial between CBV and air P%?  +19 h  ."bkha"\PRV.dial :air P%D  +18 h  P%A% h % ."bkha"\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +251 h ' ."bkha"\PRV.dial :waste2 P%) @ +75 h @ ."bkha"\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #"bkha"Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'I& h & ."bkha"Hand.open M&J1 +2 h 1 M&;& h & M&1% h %\  ."bkha"\Elbow.seek FlushPuck::Garage M&M(- +166 h (- M&#& h &- ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- ."bkha"\Forearm.seek :garage M&N'η +91 h 'η ."bkha"Hand.close M&:/ +150 h / M&E& h &- M&H% h %η ."bkha"\Forearm.seek :retract M&Q'\ +91 h '\  ."bkha"\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&&& h &ӷ ."bkha"\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ ."bkha"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' ."bkha"\CC.seek :closed C'C(W +416 h (W C'>& h &W ."bkha"Hand.open M&.1 +150 h 1 M&F% h % ."bkha"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  C'%& h &W #"bkha"\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%[ @ +75 h @ ."bkha"delay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h ."bkha"delay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  ."bkha"delay 2 seconds +200 P%P% h % ."bkha"\PS.seek 1.499ml P% ' +1597 h ' ."bkha"delay 5 seconds +500 .\PS.seek 2.5ml P%B' +1094 h ' ."bkha"\PSV.dial :air P%t  +38 h  ."bkha"\PS.seek 0ml P%^' +2609 h ' #"bkha"\mixing lysate with speDil .\PSV.dial :PML P%W +38 h ."bkha"\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%a  +38 h  ."bkha"delay 2 seconds +200 P%% h % ."bkha"\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %f'r +2309 h 'r ."bkha"delay 5 seconds +500 .\PRV.dial CBV P%U  +76 h ."bkha"delay 2 seconds +200 P%!% h %r ."bkha"\PS.seek 0.475ml P%i' +2562 h ' ."bkha"delay 5 seconds +500 .\PRV.dial :air P%e  +38 h  ."bkha"delay 2 seconds +200 .\PS.seek 0.965ml P%Y'N +4991 h 'N ."bkha"delay 10 seconds +1000 .\PRV.dial CBV P%l  +38 h ."bkha"\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%m  +37 h  ."bkha"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %o' +1632 h ' ."bkha"delay 3 seconds +300 .\PSV.dial PTV P%b +75 h ."bkha"\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ ."bkha"\PS.seek 0ml P%4' +2610 h ' ."bkha"\PSV.dial :air P%r  +75 h  ."bkha"\PS.seek 2.5ml P%q' +2610 h ' ."bkha"delay 3 seconds +300 .\PSV.dial PTV P%k +75 h ."bkha"\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%n' +2610 h ' #"bkha"\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%8  +75 h  ."bkha"\PS.seek 2.5ml P%v' +2610 h ' ."bkha"delay 3 seconds +300 .\PSV.dial :PML P%w +38 h ."bkha"\PTV.dial :PRVmixing,avoiding: :puck P%y  +75 h P%{% h % ."bkha"\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %j' +7722 h ' ."bkha"delay 5 seconds +500 .\AIV.dial :sample C'd & +50 h & ."bkha"\AWV.select :waste C' +7 P%|% h % ."bkha"\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %`'nC +10547 h 'nC ."bkha"delay 15 seconds +1500 .\AIV.dial :bypass C'K { +50 h { ."bkha"\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' ."bkha"\PSV.dial :air P%L  +37 h  ."bkha"\PS.seek 2.5ml P%' +2610 h ' ."bkha"\PSV.dial :PML P% +38 h ."bkha"\PS.seek 0ml P%~' +2609 h ' ."bkha"\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #"bkha"Beginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'z& ."MFBPCR"\ASV.dial :SPEdelivery h &W F*] @@T *  ."bkha"\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #"bkha"\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 ."bkha"\PS.seek 1.5ml P%X' +1597 h ' ."bkha"delay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 ."bkha"\PSV.dial :waste1 P%  +38 h  ."bkha"\PS.seek 0ml P%' +1597 h ' ."bkha"\PSV.dial PSR P%} +38 h ."bkha"\PSR.select :air P% +6 ."bkha"\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV ."bkha"\CBV.dial PRV .\PSV.dial :mixloop P%\ +113 h ."bkha"\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' ."bkha"\PSV.dial PSR P% +21 h  F*Z% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #"bkha"\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 ."bkha"\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' ."bkha"delay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  ."bkha"\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*,% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' ."bkha"\PSV.dial PSR P%* +37 h ."bkha"\PSR.select :air P% +7 ."bkha"\PS.seek 2.5ml P%/' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV ."bkha"\CBV.dial PRV .\PSV.dial :mixloop P% +113 h ."bkha"\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%0' +2610 h ' ."bkha"\PSV.dial PSR P%' +113 h P%% h % #"bkha"\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 ."bkha"\PS.seek 1.5ml P%' +1597 h ' ."bkha"delay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  ."bkha"\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*+9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' ."bkha"\PSV.dial PSR P%_ +38 h ."bkha"\PSR.select :air P% +6 ."bkha"\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV ."bkha"\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h ."bkha"\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%<' +2609 h ' ."bkha"\PSV.dial PSR P%9 +113 h P%?% h % #"bkha"\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 ."bkha"\PS.seek 1.5ml P%D' +1597 h ' ."bkha"delay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  ."bkha"\PS.seek 0ml P%$' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' ."bkha"\PSV.dial PSR P%) +37 h ."bkha"\PSR.select :air P% +7 ."bkha"\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +2382 h ' ."connect"\PRV.dial CBV ."bkha"\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h ."bkha"\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %;'k +2679 h 'k ."bkha"\PSV.dial :waste1 P%1  +150 h  ."bkha"\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *"'V +849 h ' ."bkha"\PSV.dial :air P%:  +113 h  ."bkha"\PS.seek 2.5ml P%E' +2610 h ' ."bkha"\PSV.dial :mixloop P%H +38 h ."bkha"\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %5' +5106 h ' ."bkha"\PSV.dial :air P%N  +38 h  ."bkha"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %&' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*V  +43 h  F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *Q'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*.' +171 h ' ."bkha"\PSV.dial :mixloop P%F +37 h ."bkha"\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +619 h ' ."MFBPCR"\ATV.dial :waste F*[ n +128 h n ."MFBPCR"\AS.seek :empty F*O'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*\\  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F* % h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #"bkha"\Cleaning Collection Syringe .\CSV.dial CSR C'B  +38 h  C'W% h %4 #"bkha"\Pulling 4ml of CSR.flush .\CSR.select :flush C' !"bkha"o:UserAbort:bt[ "7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg":@checkpoint: omitted #-> abort! "test" !o:CmdServer::Client::Error:bt[" bkha:3:in `topLevelBinding'" abort! "test" !o:CmdServer::Client::Error:bt[" bkha:4:in `topLevelBinding'" execute '18tun_postdeploy2.rb' !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"GNo '18tun_postdeploy2.rb' script found on /home/esp/esp2/mission:. #-> exit #m\EXIT @1542781127.87CET @1542781219.60CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy2.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy2 w/pucks in tube 4\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:20:27, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy2 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tun_postDeploy2.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:20:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:21:02, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 200ml, wcr at most 200ml .BACfans.suppressMail "Started mfb in tube 4 at 07:21:02CET21-Nov-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:21:02, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil M&11 F* +6 *2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down A(H'X M&I& h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 200ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'+9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'H9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'89 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'4 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'` +113 h .t\CBV.dial CSV C'a  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +38 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +131 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (i( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (o& h & M&^& h &ӷ M&q% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &r( +142 h ( M&s& h & A(t& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &u' +89 h ' A(v& h & .t\SE.seek 20.71 Pucks below Top Plate A(w' +57 h ' M&n& h & A(y& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &z' +25 h ' .t\Elbow.hold M&}, +7 h , .t\Elbow.reconfigure TightElbow A(|- h -+ M&$&.,(\ #b .t\SE.seek 21.04 Pucks below Top Plate A( ' +27 h ' M&% h % A({& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 22.04 Pucks below Top Plate A(' +36 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +90 h '\  .t\Elbow.seek CC M&~(ӷ +142 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A(& h & A(& h & M& & h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M& , +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'B +21 h 'B M&"& h & A((& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &+' +22 h ' A(% h % ."downElevator"\SE.seek :down A(*'X +44 h 'X ."downElevator"\SE.coast A(5 +56 (,% h %X M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C' @ +19 h @ .t\CS.seek 10ml C''' +7 h ' .t\CSV.dial between air and CTV C'/ ` +19 h ` .t\AIV.dial :bypass C'2 { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'3& h & .tHand.open M&11 +2 h 1 M&5& h & M&6% h %\  .t\Elbow.seek FlushPuck::Garage M&%(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &$& h &- .t\Forearm.seek :garage M&9'η +91 h 'η .tHand.close M&0/ +150 h / M&<& h &- M&=% h %η .t\Forearm.seek :retract M&?'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &:(ӷ +264 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &E' +91 h ' .t\CC.seek :closed C')(W +416 h (W C'B& h &W .tHand.open M&I1 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%& +151 h P%J% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%;' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%>  +38 h  .t\PS.seek 0ml P%#' +1597 h ' .t\PSV.dial PSR P%N +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%M' +2610 h ' ."connect"\PRV.dial CBV P%F  .t\CBV.dial PRV C'@  h +75 .t\PSV.dial :mixloop P%H +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%K  +113 h .t\PS.seek 0ml P%C' +2609 h ' .t\PSV.dial :air P%Q  +38 h  .t\PS.seek 2.5ml P%.' +2610 h ' .t\PSV.dial :mixloop P%U +37 h .t\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial :air P%R  +38 h  .t\PS.seek 2.5ml P%8' +2609 h ' .t\PSV.dial :mixloop P%Y +38 h .t\PS.seek 0ml P%T' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&!& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +6 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C'Z( +416 h ( M&`& h &ӷ M&a% h % .t\Forearm.seek :retract M&]'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &_(- +265 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &c'η +91 h 'η .tHand.open M&g1 +150 h 1 M&X% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &f'\ +90 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'j& h & M&k% h %\  A(l% h %X A(m. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (h(,9 +201 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (o& h &,9 M&^& h &- M&q% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &s(w +166 h (w M&t& h &w A(v& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &n' +89 h ' A(y& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(L' +66 h ' A(|& h &,9 M&& h &w .t\Elbow.seek 4 M&{(w +7 h (w M&& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&S& A(u'X h &w M&r% h %7 .t\Forearm.seek :retract M&w'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&z(ӷ +142 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&1 +150 h 1 M&}% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'x  +151 h  .t\CSV.dial CTV C'P  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C' '4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%~ +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'"  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'( @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C' ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C',' +2647 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2648 h '4 .t\CBV.dial :waste1 C'/ @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&% h %\  A(2% h %X A(3. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (1( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (5& h & M&6& h &ӷ M&*% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &+( +143 h ( M&& h & A($& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h & .t\SE.seek 21.71 Pucks below Top Plate A( ' +47 h ' M&0& h & A(<& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &=' +26 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(9- M&$&.,(\ #b h -+ .t\SE.seek 22.04 Pucks below Top Plate A(A' +27 h ' M&D% h % A(%& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&?'B +20 h 'B M&& h & A(B& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &I' +23 h ' .t\SE.seek 23.04 Pucks below Top Plate A(7'x +36 h 'x M&&& h & M&J% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +89 h '\  .t\Elbow.seek CC M&>(ӷ +143 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&#' +91 h ' .tHand.close M&F/ +150 h / .t\CC.seek :open C'@( +416 h ( A(H& h & A(K& h & M&M& h &ӷ M&Q% h % .t\Forearm.seek :retract M&C'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &U( +143 h ( M&.& h & A(R& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &V' +92 h ' .t\Elbow.hold M&Y, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &81 +150 h 1 M&!% h % A([& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &\\'B +20 h 'B M&4& h & A(:& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &G' +22 h ' .t\SE.seek :down A()'X +34 h 'X .t\SE.coast A(5 .t\SE.seek :down + A(`'X +7 h 'X .t\SE.coast A(5 M&a% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +89 h '\  !to:Puck::Error:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"ETube 3 overflow! Before shutdown:\ Puck.move 3, :@checkpoint: omitted #-> exit #m\EXIT @1542781488.35CET @1542781764.16CET = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:29:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: postDeployPhase\\n/home/esp/esp2/mission/18tun_postDeploy.rb:104\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:29:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:29:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy phase postDeployPhase" #\Phase [postDeployPhase] #==================> STARTED postDeploycoreNegNTC (phase #1) <=== #\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 4 at 07:29:53CET21-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:29:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&I& A(H'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'T& h &W ."airStroke"\CSV.dial :waste S)U% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors C'V'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X9 h 9d +100 C'M9 h 9 d +100 C'N9 h 9$d +100 C'F9 h 9(d +100 C'@9 h 9 ,d +100 C'H9 h 9$0d +100 C'C9 h 9<4d +100 C'K9 h 9S8d +100 C'.9 h 9kd +100 C'e9 h 9Td +100 C'f9 h 9kd +100 C'g9 h 9d +100 C'h9 h 9d +100 C'i9 h 9d +100 C'_9 h 9d +100 C'Y9 h 9d +100 C'k9 h 9d +100 C'l9 h 9 d +100 C'm9 h 9 d +100 C'd9 h 96d +100 C'o9 h 9Ld +100 C'`9 h 9bd +100 C'q9 h 9xd +100 C'r9 h 9d +100 C's9 h 9d +100 C'n9 h 9d +100 C't9 h 9d +100 C'v9 h 9d +100 C'w9 h 9d +100 C'y9 h 9d +100 C'u9 h 9(d +100 C'{9 h 9>d +100 C'j9 h 9Td +100 C'z9 h 9id +100 C'x9 h 9d +100 C'|9 h 9d +100 C'~9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'}9 h 9  d +100 C'9 h 9 d +100 C'9 h 9 *d +100 C'9 h 9 ?d +100 C'9 h 9 Td +100 C'9 h 9 j d +100 C' 9 h 9 $d +100 C'\ 9 h 9 (d +100 C' 9 h 9 ,d +100 C' 9 h 9 0d +100 C'9 h 9 4d +100 C'9 h 9 8d +100 C'9 h 9   +18 h C'M% h %4 .t\CS.seek 5ml C'N'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'F  +37 h C'@% h %p .t\CS.seek 7ml C'H'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'C @ +37 h @ .t\CS.seek 10ml C'K' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'. ` +132 h ` #tLysing Complete C'Q& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&R% h %\  A(T% h %X A(U. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (X( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (O& h & M&8& ."raiseStack"\SE.seek :upperLimit A( 'Z h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &!(ӷ +7 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C']( +23 h 'Z +393 ( A(^& h & A(W& h & M&4& h &ӷ M&a% h % .t\Forearm.seek :retract M&b'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &c( +142 h ( M&e& h & A(f& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &g' +93 h ' .t\Elbow.hold M&h, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &i1 +150 h 1 M&_% h % A(Y& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &k'B +20 h 'B M&l& h & A(m& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +22 h ' A(o% h %Z ."downElevator"\SE.seek :down A(`'X +100 (q% h % M&r% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'n @ +2 A(6 +17 h @ .t\CS.seek 10ml C't' +6 h ' .t\CSV.dial between air and CTV C'v ` +19 h ` .t\AIV.dial :bypass C'w { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'y& h & .tHand.open M&u1 +2 h 1 M&{& h & M&j% h %\  .t\Elbow.seek FlushPuck::Garage M&z(- +166 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.seek :garage M&~'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&}'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&\ 1 +150 h 1 M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%  .t\CBV.dial PRV C'  h +76 .t\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +112 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.seek 2.5ml P%+' +2610 h ' .t\PSV.dial :mixloop P%" +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &,(ӷ +6 h (ӷ M&*& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&'' +91 h ' .tHand.close M&// +150 h / .t\CC.seek :open C'%( +416 h ( M&2& h &ӷ M&0% h % .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &1(- +264 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &6'η +91 h 'η .tHand.open M&$1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'<& h & M&9% h %\  A(?% h %X A(D. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (B(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (A& h &,9 M&=& h &- M&)% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &G(w +166 h (w M&I& h &w A(J& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &&' +90 h ' A(;& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(>' +66 h ' A(M& h &,9 M&#& h &w .t\Elbow.seek 4 M&F(w +6 h (w M&@& h &w A(N& h &,9 .t\Forearm.seek 4 + 300 counts M&C'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &E/ +150 h / ."downElevator"\SE.seek :down M&H& A(.'X h &w M&Q% h %7 .t\Forearm.seek :retract M&R'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&T(ӷ +143 h (ӷ M&U& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &8' +91 h ' .t\CC.seek :closed C'[(W +416 h (W .tHand.open M&Z1 +150 h 1 M&\\% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'^'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'W' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'4'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%a  +37 h  .t\PS.seek 2.5ml P%e' +2610 h ' .t\PSV.dial :mixloop P%f +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%V  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%! +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%i  +151 h  .t\PS.seek :empty P%_' +2609 h ' .t\CBV.dial :waste1 C'Y @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'l  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'm @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'o  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'b ` +19 h ` .t\CSV.dial :air C'c @ +37 h @ .t\CS.seek 10ml C'q' +2647 h ' .t\CSV.dial CTV C'r  +38 h  .t\CS.seek 0ml C'g'4 +2647 h '4 .t\CBV.dial :waste1 C'k @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'n  +151 h C' & h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&v% h %\  A(w% h %X A(y. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (u( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b ({& h & M&j& h &ӷ M&d% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &x( +143 h ( M&|& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(K& h & .t\SE.seek -0.29 Pucks below Top Plate A(h'\\ +264 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&s, +6 h , .t\Elbow.reconfigure TightElbow A(~- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&% h % A(z& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&}'B +20 h 'B M&]& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &\ ' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A( ' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +90 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( A( & h & A(& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +143 h ( M&& h & A((& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&", +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &+1 +150 h 1 M&% h % A(*& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &'B +21 h 'B M&/& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h % ."downElevator"\SE.seek :down A(2'X +100 (0% h %S M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%7  + A(6 +112 h  P%,% h % .t\PS.seek 0.499ml P%$' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +38 h .t\PRV.dial :air P%3  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%<  +37 h .t\CBV.dial AIV C'9  +38 h .t\PRV.dial between CBV and air P%?  +19 h  .t\PRV.dial :air P%D  +18 h  P%A% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %=' +251 h ' .t\PRV.dial :waste2 P%) @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %6' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'I& h & .tHand.open M&J1 +2 h 1 M&;& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&M(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &@& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&:/ +150 h / M&E& h &- M&H% h %η .t\Forearm.seek :retract M&Q'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +264 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'C(W +416 h (W C'>& h &W .tHand.open M&.1 +150 h 1 M&F% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &R'\ +91 h '\  C'%& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Z @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +76 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%8' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%B' +1094 h ' .t\PSV.dial :air P%t  +38 h  .t\PS.seek 0ml P%^' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%W +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%a  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %f'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%V  +76 h .tdelay 2 seconds +200 P%!% h %r .t\PS.seek 0.475ml P%i' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%e  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Y'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%l  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%m  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %o' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%b +75 h .t\PTV.dial :PRV,avoiding: :puck P%c @ +75 h @ .t\PS.seek 0ml P%4' +2610 h ' .t\PSV.dial :air P%r  +75 h  .t\PS.seek 2.5ml P%q' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%k +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%n' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P%v' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%w +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%y  +75 h P%{% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %j' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'd & +50 h & .t\AWV.select :waste C' +7 P%|% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %`'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'K { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %T' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%~' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C F*] @@T C'z& ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +92 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*x I +129 h I F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*[% h %6 ."MFBPCR"\AS.seek 0.329ml F* 'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +526 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  .t\PS.seek 0ml P%' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*+% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +40 h ' .t\PSV.dial PSR P%* +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%/' +216 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%0' +2610 h ' .t\PSV.dial PSR P%' +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%7' +739 h ' ."MFBPCR"delay 5 seconds +500 F*,9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F* n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*2% h % ."MFBPCR"\AS.seek 0.041ml F*u'% +336 h ' .t\PSV.dial PSR P%_ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%3 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%<' +2609 h ' .t\PSV.dial PSR P%9 +113 h P%?% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%D' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%A  +38 h  .t\PS.seek 0ml P%$' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%) +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%=' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%J +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %;'k +2679 h 'k .t\PSV.dial :waste1 P%1  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*@ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *"'V +849 h ' .t\PSV.dial :air P%:  +113 h  .t\PS.seek 2.5ml P%E' +2610 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %5' +5106 h ' .t\PSV.dial :air P%N  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %&' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*U  +43 h  F*M% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *Q'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*>% h %Q ."MFBPCR"\AS.seek 0.01ml F*.' +171 h ' .t\PSV.dial :mixloop P%F +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %%' +619 h ' ."MFBPCR"\ATV.dial :waste F*Z n +128 h n ."MFBPCR"\AS.seek :empty F*O'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*\\  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*6  +43 h F*8% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *t'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'B  +38 h  C'W% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'a'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'^  'V  +38 h  +113  .t\CBV.dial :waste1 C'! @ +38 h @ .t\CS.seek 0ml C'f'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*e  +22 h ."MFBPCR"delay 3 seconds +300 F*i% h %_ ."MFBPCR"\AS.seek 0.434ml F*l'ݏ +110 h '4 .t\CSV.dial CSR C'm  +38 h  C'Y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'b'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*c  +22 h F*o% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*r'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*4 n +107 h n F*k% h %& ."MFBPCR"\AS.seek 0.092ml F*q'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'w'4 +2647 h '4 C'y& h &W #tGaraging FlushPuck in Collection Clamp M&{& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M&K/ +150 h / .t\CC.seek :open C'`( +416 h ( M&L& h &ӷ M&T% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 4 into Collection Clamp C'x& h & M&h% h %\  A(% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (X(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (}& h &,9 M&& h &- M&\ % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&& h &w A([& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A(' +76 h ' A(s& h &,9 M&& h &w ."WCR"\Elbow.seek 4 M& (w +7 h (w M&(& h &w A(& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&+'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(*'X h &w M&% h %7 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &/' +91 h ' ."WCR"\CC.seek :closed C''(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *0'R +370 h (W ."WCR"Hand.open M& 1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *2' +29 h '\  ."WCR"\SC.coast #collectSample 0ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)_% h % C'7& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'3  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +94 h  C'F% h % ."WCR"\CSV.dial between air and CTV C'Q ` +19 h ` ."WCR"\CS.seek 7.288ml C'&' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'Z  ."WCR"\CSV.dial CTV C'.  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '\\'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'S  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'O' +41 h ' ."WCR"delay 1:00 +5837 h ' ."MFBPCR"delay 5 seconds +163 ."WCR"\CTV.dial :air,avoiding: :intake C'6  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'8  +38 h  C'P% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'B' +143 ."MFBPCR"\ATV.dial ASV F*W n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*g' +399 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'a' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'^ @ +75 h @ +50 S)V% h % ."WCR"\CBV.dial between CSV and waste2b C'!  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'e ` +57 h ` ."WCR"\CS.seek 7.288ml C'i' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'm  'Y  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 't'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'f  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'c' +41 h ' ."WCR"delay 1:00 +3122 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*o  +65 h  F*l% h % ."MFBPCR"\AS.seek 0.52ml F*4' +2313 ."WCR"\CTV.dial :air,avoiding: :intake C'k  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'r  +37 h  C'b% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'n' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'y @ +76 h @ +50 S){% h % ."WCR"\CBV.dial between CSV and waste2b C'd  +94 h  C'|% h % ."WCR"\CSV.dial between air and CTV C'K ` +56 h ` ."WCR"\CS.seek 7.288ml C'G' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'R  'C  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'L'4 +1919 h '4 ."WCR"delay 2:00 +2814 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*T n +64 h n ."MFBPCR"\ASV.dial :wasteR F*j  +21 h  ."MFBPCR"\AS.seek 0.3ml F*': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*z'w +2155 ."WCR"\CBV.dial :waste2b C'v  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +2432 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*x  +87 h F*h% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *'N +1005 ."WCR"\CTV.dial :air,avoiding: :intake C'p  +22 h 'N ."MFBPCR"delay 3 seconds +129 h C'}& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&% h %\  A(\ % h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +171 ."MFBPCR"\ATV.dial :ElutionWater F*[ I +29 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&s% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &( +14 h I F*(% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +129 h ( M&~& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A('K +253 h 'K M&& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' ."WCR"\Elbow.hold M&+, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&*% h % A( & h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&`& h & A(q& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b & ' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(' +36 h ' M& & h & M&_% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +89 h '\  ."WCR"\Elbow.seek CC M&3(ӷ +143 h (ӷ M&/& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &0& h &ӷ ."WCR"\Forearm.seek CC M&X' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'?( +144 h '{ ."MFBPCR"delay 3 seconds +272 h ( A(9& h & A(<& h & M&A& h &ӷ M&D% h % ."WCR"\Forearm.seek :retract M&'\ +28 ."MFBPCR"\ATV.dial ASV,via: :air2 F*$ n +63 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &u( +109 h n ."MFBPCR"\ASV.dial :SPEelution F*J  +33 h ( M&)& h & A(1& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &;' +10 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *@% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *I' ?x +76 h ' ."WCR"\Elbow.hold M&:, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &=1 +150 h 1 M&E% h % A(N& h & ."WCR"\Forearm.force -20 .\Forearm.seek 3 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &5'B +20 h 'B M&#& h & A(U& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &w' +22 h ' A("% h % ."downElevator"\SE.seek :down A('X +100 (>% h %B M&F% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +90 h '\  +51 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'M& h & .tHand.open M&.1 +2 A(6 h 1 M&Z& h & M&&% h %\  .t\Elbow.seek FlushPuck::Garage M&S(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &6& h &- .t\Forearm.seek :garage M&8'η +91 h 'η .tHand.close M&P/ +150 h / M&W& h &- M&O% h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +265 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &!' +91 h ' .t\CC.seek :closed C'(W +415 h (W C'e& h &W .tHand.open M&a1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'i& h &W C'f% h % .t\CSV.dial CSR C't  +38 h  C'o% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'l'p +1227 h ' ?x ."MFBPCR"delay 3 seconds +146 h 'p .tdelay 5 seconds +154 ."MFBPCR"\AFV.select :holdingCoil F* +6 *g% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *k' +340 .t\CSR.select :air C' +6 'r% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'b'[ +463 h '[ .t\CSV.dial :waste C'c +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'n'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'y& h &W C'{% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C' % h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV C'C  .t\CTV.dial CSV C'G  +37 h  +113  .t\CBV.dial :waste1 C'T @ +75 h @ .t\CS.seek 0ml C'j'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'4& h &W C'% h %4 .t\CSV.dial :flush C'v  +76 h  .t\CS.seek 5ml C'L'p +76 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *z' +74 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'x @ +38 h @ C'h% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'p  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'}  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'\  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +470 h ' ."MFBPCR"delay 1:00 +30 .t\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV C's  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'[ @ +75 h @ .t\CS.seek 0ml C'('4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'~  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '*'P +788 #"MFBPCR"pushing eluate to holding coil F* % h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +328 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'_  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'/'4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +37 h @ C'% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'' +113 h .t\CTV.dial :air .\CS.seek 0ml C'9'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'A +37 h .t\CS.seek 0ml C'D'4 +507 h '4 C'$& h &W C')% h %4 .t\CSV.dial :flush C'1  +37 h  .t\CS.seek 3ml C'J'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'3'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'='4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'E'0 +506 h '0 .t\CSV.dial :waste C'N +76 h .t\CS.seek 0ml C'#'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'U& h &W C'"% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'u @ +38 h @ C'>% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  '.  +38 h  +112  .t\CBV.dial :waste1 C'Z @ +38 h @ .t\CS.seek 0ml C'&'4 +766 h '4 #t\Flushing lower manifold's waste2b port C';& h &W C'5% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +651 h 'p .tdelay 5 seconds +108 h 'Q ."MFBPCR"delay 30 seconds +392 .t\CSV.dial :air C'\\ @ +37 h @ C'6% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C':  +75 h  .t\CS.seek 0ml C'P'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&W& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &O(ӷ +7 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&^' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'S( +416 h ( M&2& h &ӷ M&?% h % .t\Forearm.seek :retract M&e'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &a(- +265 h (- M&Y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &f'η +91 h 'η .tHand.open M&t1 +150 h 1 M&o% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +16 ."MFBPCR"\ATV.dial :waste,via: :air3 F* n +75 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'g& h & .tHand.open M&I1 +2 h 1 M&r& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &l(- +6 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&y'η +68 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +23 h 'η .tHand.close M&d/ +150 h / M&n& h &- M&K% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &|(ӷ +265 h (ӷ M&C& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &T' +90 h ' .t\CC.seek :closed C'R(W +416 h (W C'4& h &W .tHand.open M&1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &j'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'k% h %4 .t\CSV.dial CSR C'x  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'L'p +314 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*p % +43 h % F*}% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*\ I +21 h I F* % h %v ."MFBPCR"\AS.seek 0.059ml F*'H +369 h 'p .tdelay 5 seconds +256 h 'H ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*s% h %H ."MFBPCR"\AS.seek 0.069ml F*[' +222 h ' ."MFBPCR"delay 2 seconds + .t\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '~'[ +193 ."MFBPCR"\ATV.dial :bleach F*  +22 h F*(% h % ."MFBPCR"\AS.seek 0.769ml F*' q +248 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'z '  +38 h +38 .t\CTV.dial :intake C'*  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'_'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'  +76 h #t\Flushing upper manifold C'& h &W C'q% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'0% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'/'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C' '4 +506 h '4 C''& h &W C'7% h %4 .t\CSV.dial :flush C'9  +38 h  .t\CS.seek 3ml C'A'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'< @ +37 h @ C'$% h %0 .t\CS.seek 5ml C')'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'1'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'D'0 +506 h '0 .t\CSV.dial :waste C'@ +75 h .t\CS.seek 0ml C'X'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&J& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +7 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.seek CC M&E' +90 h ' .tHand.close M&U/ +150 h / .t\CC.seek :open C'"( +416 h ( M&& h &ӷ M&#% h % .t\Forearm.seek :retract M&u'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &>(- +96 h ' q ."MFBPCR"delay 3 seconds +169 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &.& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &M'η +91 h 'η .tHand.open M&Z1 +40 ."MFBPCR"\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +88 h 1 M&;% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &5'\ +91 h '\  #t\Completed [postDeployPhase] begun 01:00:31 #\Extending 18tun_postDeploy until completion of MFBPCR +121 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*&' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +22 h F*6% h % k ."MFBPCR"\AS.seek 0.779ml F*w'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *:' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*W'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*8% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *V' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*m% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %  ."MFBPCR"\AS.seek 0.604ml F*^'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*i% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*O' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*t n +86 h n ."MFBPCR"\ASV.dial :wasteR F*o  +43 h  ."MFBPCR"\AS.seek 0ml F*e'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*r % +108 h % F*c% h %V ."MFBPCR"\AS.seek 0.59ml F*b' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*f% h %  ."MFBPCR"\AS.seek 0.09ml F*d' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*K I +86 h I F*a% h % ."MFBPCR"\AS.seek 0.12ml F*!' +625 h ' ."MFBPCR"\ATV.dial :air3 F*` % +21 h % F*y% h % ."MFBPCR"\AS.seek 0.13ml F*C' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*l% h % ."MFBPCR"\AS.seek 0.23ml F* ' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*k' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*h  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *P% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *p'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*} @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*\ I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*(% h %(A ."MFBPCR"\AS.seek 0.6ml F*L'\  +1031 h '\  F*[% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*~% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*,  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*z  +11 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*_  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*/' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.53ml F*'' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*7 % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*9% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +108 h  ."MFBPCR"delay 7 seconds +700 F*$% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*) n +21 h n ."MFBPCR"delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.6ml F*@'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*N  +43 h  ."MFBPCR"\ASV.dial :waste1 F*U  +65 h ."MFBPCR"\ARV.dial :air1 F*|  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*j% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr F*E% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*.  +22 h ."MFBPCR"\ATV.dial :air3 F*3 % +86 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*&% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*:' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*?  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*g'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*c% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0104h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ARV.dial :carrier F*S  +11 h  F*4% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*k  +22 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*p  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*s' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*['\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*L n +22 h n ."MFBPCR"delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F**% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0104h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*_  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*9% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*<  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*$  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *)'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*U  +31 h  ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*j% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*#% h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % ."MFBPCR"delay 3 seconds +300 F*R% h %> ."MFBPCR"\AS.seek 0.835ml F*.'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +21 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*;  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*Z' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*8  +10 h  F*:% h %V ."MFBPCR"\AS.seek 0.5ml F*W'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*m n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *V'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Y  +22 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *^'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*t n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*i% h %Fj ."MFBPCR"\AS.seek 0.524ml F*O' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*e I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.53ml F*r' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +108 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*n n +21 h n ."MFBPCR"delay 2 seconds +200 F*K% h %\  ."MFBPCR"\AS.seek 0.6ml F*a'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*`% h %\  ."MFBPCR"\AS.seek 0.65ml F*y' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*!  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*C% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*v  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F*p'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*s  +65 h  F*(% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b **' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*0'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *$'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ARV.dial :carrier F*'  +11 h  F*)% h %V ."MFBPCR"\AS.seek 0.5ml F*1'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*U  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*|  +22 h ."MFBPCR"delay 2 seconds +200 F*j% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *N'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*#% h %Fj ."MFBPCR"\AS.seek 0.524ml F*=' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*E n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*R% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*;  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Z n +21 h n ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*6% h %\  ."MFBPCR"\AS.seek 0.65ml F*&' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*\\  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*: % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h.pcr F*Q% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*m  +22 h ."MFBPCR"\ATV.dial :air3 F*B % +86 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*2% h %Z ."MFBPCR"\AS.seek 0.81ml F*?'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*i% h %> ."MFBPCR"\AS.seek 0.835ml F*^'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*g  +64 h  F*r% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *I'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*a % +86 h % F*!% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *G' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*y% h % ."MFBPCR"\AS.seek 0.81ml F*4'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*S'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*h'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0111h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*(  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*~ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *['R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*, I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*} % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*_  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*q  +22 h  ."MFBPCR"delay 7 seconds +700 F*0% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*+  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*7' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*9  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*/  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*'  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0111h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*$ @@T ."MFBPCR"\ASV.dial :SPEdelivery F*1  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*A  +151 h F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *U' +423 h ' ."MFBPCR"\ATV.dial :ASV F*| n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*j  +43 h  F*@% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*# n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *N'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *E'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*R'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*= % +43 h % F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +423 h ' ."MFBPCR"\ATV.dial :air3 F*]% h % ."MFBPCR"\AS.seek 0.53ml F*;' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*.% h % ."MFBPCR"\AS.seek 0.71ml F*Z' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*6  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*\\' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*&' +7161 h ' ."MFBPCR"\ATV.dial :waste F*8 n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*Q % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*B  +22 h F*V% h % ."MFBPCR"\AS.seek 0.579ml F*W' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*t  +21 h F*i% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*?' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*e n +86 h n ."MFBPCR"\ASV.dial :wasteR F*o  +22 h  ."MFBPCR"\AS.seek 0ml F*^'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *g'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*r % +108 h % F*O% h %V ."MFBPCR"\AS.seek 0.01ml F*I' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*n' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h % q ."MFBPCR"\AS.seek 0.605ml F*`'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*!% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*d' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*C n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*G'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*4  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *v' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *S'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +202 h 'R ."MFBPCR"\SPE.coast F*P  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*x'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*k n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*\  +42 h  F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *p' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*~ n +65 h n F*L% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *[' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*}% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F** n +65 h n F*_% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*0  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*+'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*9% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b */' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*7% h % ."MFBPCR"\AS.seek 0.96ml F*''0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %0 ."MFBPCR"\AS.seek 0.985ml F* ' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +22 h F*J% h % ."MFBPCR"\AS.seek 0.97ml F*D'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*| I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *@'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *#'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *N'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*E  +43 h  F*=% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *3' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *R' +9988 h ' ."MFBPCR"\ATV.dial AIV F*] I +43 h I ."MFBPCR"\AS.seek 0.02ml F*F' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*.'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*;% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*u  +22 h ."MFBPCR"\ATV.dial :air3 F*Z % +43 h % F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*:'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*B  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*V  +64 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*o  +32 h  ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*^% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *c' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +21 h F*O% h % ."MFBPCR"\AS.seek 0.81ml F*g'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*n'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *S'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*h % +108 h % ."MFBPCR"\AS.seek 0ml F*k'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0115h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*\  +64 h  ."MFBPCR"\ARV.dial :carrier F*T  +11 h  F*x% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*{ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*L% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*}% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F** I +108 h I ."MFBPCR"delay 2 seconds +200 F*_% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0115h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*+  +22 h ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b */'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*)  +21 h F*1% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*D% h %n ."MFBPCR"\AS.seek 0.82ml F*j'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*U  +65 h  F*#% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*; % +87 h % F*F% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.81ml F*6'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*w'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\\% h %m ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *t'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0117h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*o% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *r'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*c  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *g'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*K  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*b% h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*a  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*!' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*`  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.536ml F*y'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*G% h %< ."MFBPCR"\AS.seek 0.594ml F*l'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*4 I +107 h I ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*h  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*x  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*k % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0117h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*p  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*(% h %Z ."MFBPCR"\AS.seek 0.81ml F*~'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*,  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*q % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*7'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0119h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*#  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*E'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *3'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*;  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *R'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*u n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*Z% h %Fj ."MFBPCR"\AS.seek 0.524ml F*.' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*M I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*6' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.536ml F*w'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*Q'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*Y  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*W% h % \\ ."MFBPCR"\AS.seek 0.326ml F*i'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*o % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0119h.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*e  +22 h ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*r% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *c'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*O'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*K % +21 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*a  +21 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*C  +65 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*P  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*x'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *s'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0121h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*_ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*q  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*0 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*$ n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +108 h  ."MFBPCR"delay 7 seconds +700 F*7% h %< ."MFBPCR"\AS.seek 0.594ml F*)'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*1 I +108 h I ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*A'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.65ml F*|' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*@% h % \\ ."MFBPCR"\AS.seek 0.326ml F*#'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*j  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0121h.pcr F*U% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *E'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*N  +22 h ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*]'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*R'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*.'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*\\  +64 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' @CET82808.26 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*m  +32 h  ."MFBPCR"\ATV.dial :air3 F*B % +86 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *2' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*W  +21 h F*:% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*t% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*r'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*?  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *C'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0200h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*!  +43 h  ."MFBPCR"\ARV.dial :carrier F*I  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*G'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*4 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *l'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*\ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*S' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*k % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*x' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*T'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*{  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*s'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*( I +108 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*,% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0200h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*_  +21 h ."MFBPCR"\ATV.dial :air3 F** % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*+  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*<  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *9'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*'  +31 h  ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*@  +22 h F*|% h % ."MFBPCR"\AS.seek 0.81ml F*j'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*E  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +5060 h 'V #t\Asleep after completing 18tun_postDeploy assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:30:38, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy assays" #\Mission runtime was 2:39.34769 #-> execute '18tun_postDeploy2.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy2 w/pucks in tube 4\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:30:38, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy2 begun" #\Preparing to sleep until +0:00:15 ."shutdown"\AS.coast .\SS.coast F*5 ."shutdown"\CC.coast S)5 #t\Asleep til +0:00:15 ."shutdown"\CS.coast C'6 #t\Continuing at 02:42 ."shutdown"\PC.coast E ;3\\0\\0 P%6 ."shutdown"\PS.coast .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tun_postDeploy2.rb:105\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:33:13, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 02:42" ."shutdown"\Forearm.coast .\Elbow.coast M&5 + &6 P%5 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown +1470 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )6 h  C' '. h  P% % h  F* *8 h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *w  h  .t\SPE.configure SPEconfig F*\ 2 *% h %V .t\AS.configure ASconfig S)&% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'Q& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:33:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 200ml, wcr at most 200ml .BACfans.suppressMail "Started mfb in tube 4 at 07:33:18CET21-Nov-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:33:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" F*% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'v& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&k1 +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F*S % +107 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *p' +37 h 1 M&% h %\  A(x% h %X A({. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ( (,9 +357 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (T& h &,9 M&(& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +19 h (w M&s& h &w A(& h &,9 .t\Forearm.seek 4 + 700 counts M&~' +89 h ' A(L& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &,9 M&,& h &w .t\Elbow.seek 4 M&[(w +6 h (w M&& h &w A(}& h &,9 .t\Forearm.seek 4 + 300 counts M& '7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&*& A(_'X h &w M&% h %7 .t\Forearm.seek :retract M&z'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&q(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M&$% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 200ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)+% h % C'<& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +800 h ' .t\Exhaust.select :close S) +66389 #t\Sampled 200.0ml #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'5& h &W ."airStroke"\CSV.dial :waste S)\\% h % ."airStroke"\CS.seek 0ml ."connect"\SSV.dial CBV,avoiding: :exhaust C'H'4 .t\CBV.dial SSV S)%  +6 h '4 ."airStroke"\CSV.dial :air C'X @ +75 h @ ."airStroke"\CS.seek 10ml C'"' +144 h  .t\CTV.dial :air C'-  +76 h .tdelay 15 seconds +690 h ' +810 .t\SSV.dial :spit,avoiding: :exhaust S)6 +150 h ."prepCSV"\CTV.dial CSV,avoiding: :intake C'.  .t\SS.seek 0ml S)' +7 h ' .t\SSV.dial CBV,avoiding: :exhaust S)8  +144 h  +6  S)w% h % .t\CBV.dial between SSV and waste2 C'  +19 h  C'&% h % ."connect"\CTV.dial CSV,avoiding: :air .t\CSV.dial CTV C'Q  +38 h  .t\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'm'4 +2610 h '4 C'B% h %4 S)Y% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'V @ +18 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0Xd +100 C'-9 h 9Td +100 C'H9 h 9kd +100 C'69 h 9d +100 C'.9 h 9d +100 C'89 h 9d +100 C'w9 h 9d +100 C'9 h 9d +100 C'&9 h 9d +100 C'Q9 h 9 d +100 C'>9 h 9 d +100 C'"9 h 96d +100 C'B9 h 9Ld +100 C'Y9 h 9bd +100 C'V9 h 9xd +100 C'9 h 9d +100 C':9 h 9d +100 C'29 h 9d +100 C'o9 h 9d +100 C'W9 h 9d +100 C't9 h 9d +100 C'e9 h 9d +100 C'^9 h 9(d +100 C'r9 h 9>d +100 C'9 h 9Td +100 C'c9 h 9id +100 C'm9 h 9d +100 C'9 h 9d +100 C'?9 h 9d +100 C'i9 h 9d +100 C'O9 h 9d +100 C'!9 h 9d +100 C'y9 h 9  d +100 C'P9 h 9 d +100 C'K9 h 9 *d +100 C'9 h 9 ?d +100 C'h9 h 9 Td +100 C'`9 h 9 j d +100 C'a9 h 9 $d +100 C'f9 h 9 (d +100 C'49 h 9 ,d +100 C'9 h 9 0d +100 C'9 h 9 4d +100 C'S9 h 9 8d +100 C'k9 h 9 '\ +89 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.seek CC M&V' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C':( +416 h ( A(2& h & A(o& h & M&W& h &ӷ M&t% h % .t\Forearm.seek :retract M&e'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &^( +143 h ( M&r& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &c' +92 h ' .t\Elbow.hold M&m, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&?% h % A(i& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &O'B +21 h 'B M&!& h & A(y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &P' +22 h ' A(K% h % ."downElevator"\SE.seek :down A('X +44 h 'X ."downElevator"\SE.coast A(5 +56 (h% h %X M&`% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +90 h '\  .t\SC.coast #Tranferring lysate to PRV A(6 .t\CSV.dial :air C'f @ +18 h @ .t\CS.seek 10ml C'4' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'S& h & .tHand.open M&k1 +2 h 1 M&& h & M&x% h %\  .t\Elbow.seek FlushPuck::Garage M&C(- +166 h (- M&b& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.seek :garage M&T'η +91 h 'η .tHand.close M&v/ +150 h / M&s& h &- M&% h %η .t\Forearm.seek :retract M&L'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &((ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &}' +91 h ' .t\CC.seek :closed C' (W +416 h (W C',& h &W .tHand.open M&*1 +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%G +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%~  +38 h  .t\PS.seek 0ml P%I' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'  h +76 .t\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%z  h .t\PS.seek 0ml P%[' +2609 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%\ ' +2610 h ' .t\PSV.dial :mixloop P%+ +37 h .t\PS.seek 0ml P%<' +2610 h ' .t\PSV.dial :air P%$  +38 h  .t\PS.seek 2.5ml P% ' +2609 h ' .t\PSV.dial :mixloop P%9 +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&g& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &1& h &ӷ .t\Forearm.seek CC M&p' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'7( +416 h ( M&A& h &ӷ M&D% h % .t\Forearm.seek :retract M&)'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &'(- +264 h (- M&|& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &@'η +91 h 'η .tHand.open M&U1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'=& h & M&#% h %\  A(E% h %X A(3. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (j(,9 +201 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (F& h &,9 M&/& h &- M&u% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &](w +166 h (w M&M& h &w A(R& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &;' +89 h ' A(5& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(q' +66 h ' A(%& h &,9 M&-& h &w .t\Elbow.seek 4 M&H(w +6 h (w M&.& h &w A(8& h &,9 .t\Forearm.seek 4 + 300 counts M&&'7 +23 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Q/ +150 h / ."downElevator"\SE.seek :down A('X M&& h &w M&Z% h %7 .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&\\(ӷ +142 h (ӷ M&B& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &6' +91 h ' .t\CC.seek :closed C'n(W +416 h (W .tHand.open M&w1 +150 h 1 M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'>  +151 h  .t\CSV.dial CTV C'  +18 h  .t\CS.seek 5ml C'2'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'o' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'W'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%t  +38 h  .t\PS.seek 2.5ml P%r' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%V  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%" +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%?' +2609 h ' .t\CBV.dial :waste1 C'i @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'!  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'y @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'K  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'e ` +19 h ` .t\CSV.dial :air C'^ @ +37 h @ .t\CS.seek 10ml C'h' +2648 h ' .t\CSV.dial CTV C'`  +37 h  .t\CS.seek 0ml C'f'4 +2648 h '4 .t\CBV.dial :waste1 C' @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'S& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&k% h %\  A(% h %X A(x. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (c( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (O& h & M&& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &P( +143 h ( M&b& h & A(d& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &N' +89 h ' A(J& h & .t\SE.seek 21.71 Pucks below Top Plate A(m' +47 h ' M&v& h & A(s& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &' +26 h ' .t\Elbow.hold M&a, +6 h , .t\Elbow.reconfigure TightElbow A(T- M&$&.,(\ #b h -+ .t\SE.seek 22.04 Pucks below Top Plate A(' +27 h ' M&% h % A(C& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &L'B +20 h 'B M&:& h & A(,& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &*' +22 h ' .t\SE.seek 23.04 Pucks below Top Plate A({'x +37 h 'x M&G& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +89 h '\  .t\Elbow.seek CC M&~(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&I' +90 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( A(_& h & A(z& h & M&& h &ӷ M&% h % .t\Forearm.seek :retract M&['\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &+( +143 h ( M&\ & h & A($& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &<' +92 h ' .t\Elbow.hold M&9, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b & 1 +150 h 1 M&g% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&1'B +21 h 'B M&& h & A((& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek :down A( 'X +33 h 'X .t\SE.coast A(5 .t\SE.seek :down +2 A(A'X +7 h 'X .t\SE.coast A(5 M&D% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +89 h '\  !to:Puck::Error:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"ETube 3 overflow! Before shutdown:\ Puck.move 3, :@checkpoint: omitted #-> exit #m\EXIT @1542782040.73CET @1542782398.21CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi$; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy2.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy2 w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:40:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy2 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tun_postDeploy2.rb:103\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:40:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:40:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 200ml, wcr at most 200ml .BACfans.suppressMail "Started mfb in tube 4 at 07:40:29CET21-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:40:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" F*/% h %&K #"readyMFB"Readying the MFB #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Homing MFB Syringe C'0& ."readyMFB"\AFV.select :holdingCoil h & F* .tHand.open M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 200ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'+9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'!9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'8 @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'4 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'` +113 h .t\CBV.dial CSV C'a  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +38 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +131 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (i( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (o& M&^& h & ."raiseStack"\SE.seek :upperLimit h &ӷ A(q'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(n& h & A(y& h & M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'9  P%$  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%<  +113 h .t\PS.seek 0ml P%=' +2610 h ' .t\PSV.dial :air P%?  +37 h  .t\PS.seek 2.5ml P%:' +2610 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%B +37 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +90 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +265 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'V& h & M&R% h %\  A(!% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (T(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (8& h &,9 M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &\\(w +166 h (w M&W& h &w A(4& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &Z' +89 h ' A(`& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(]& h &,9 M&_& h &w .t\Elbow.seek 4 M&d(w +7 h (w M&e& h &w A(c& h &,9 .t\Forearm.seek 4 + 300 counts M&g'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &X/ +150 h / ."downElevator"\SE.seek :down M&f& h &w A(j'X M&k% h %7 .t\Forearm.seek :retract M&l'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +142 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +151 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h & .t\SE.seek -0.29 Pucks below Top Plate A('\\ +265 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &"' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +26 h ' M&*% h % A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &('B +21 h 'B M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +36 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'9( +416 h ( A(0& h & A(<& h & M&& h &ӷ M&?% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &A( +142 h ( M&:& h & A(E& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &D' +93 h ' .t\Elbow.hold M&B, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h % A(&& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&J'B +21 h 'B M&>& h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  +2 A(6 +111 h  P%G% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +37 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'R  +37 h .t\PRV.dial between CBV and air P%!  +19 h  .t\PRV.dial :air P%Y  +19 h  P%8% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&41 +2 h 1 M&`& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&X& h &- M&f% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +90 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&j1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%n' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +75 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1313 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '*& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*w @@T .t\AIV.dial :bypass F*  .t\PSV.dial PSR P%2 +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%i' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*$  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*? % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%6' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2609 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +37 h  .t\PS.seek 0ml P%K' +740 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%.' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +113 h P%!% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %`'k +2678 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%X' +2610 h ' .t\PSV.dial :mixloop P%f +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5107 h ' .t\PSV.dial :air P%c  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*9 I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*j' +171 h ' .t\PSV.dial :mixloop P%d +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*o'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*^% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +1625 h ' ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV C'u  +38 h  +113  .t\CBV.dial :waste1 C'r @ +37 h @ .t\CS.seek 0ml C'{'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 200ml #Loading wcr puck from tube 4 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (i(,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &,9 M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&$& h &w A(s& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(<& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &,9 M&?& h &w ."WCR"\Elbow.seek 4 M&0(w +6 h (w M&E& h &w A(5& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&)'7 +23 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&&& A(:'X h &w M&+% h %7 ."WCR"\Forearm.seek :retract M&'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +142 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +29 h '\  ."WCR"\SC.coast #collectSample 200ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % q ."MFBPCR"\AS.seek 0.794ml F*M' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*=% h % k ."MFBPCR"\AS.seek 0.779ml F*!'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*R I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *V' +4416 h ' ."MFBPCR"\ATV.dial :waste F*8 n +128 h n ."MFBPCR"\AS.seek :empty F*Y'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*Q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*[% h % ."MFBPCR"\AS.seek 0.579ml F*@' +4172 ."WCR"\CBV.dial :waste2b C'`  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'e' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'W  +150 h C'U& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&O% h %\  A(X% h %X A(c. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (I( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (h& h & M&_& h &ӷ M&f% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &B( +143 h ( M&9& h & A(a& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &d' +89 h ' A(k& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(]'K +254 h 'K M&t& h & A(N& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &v' +25 h ' ."WCR"\Elbow.hold M&S, +7 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(j- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(C' +26 h ' M&^% h % A(o& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&P'B +21 h 'B M&y& h & A(T& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &}' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(n' +36 h ' M&& h & M&r% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  ."WCR"\Elbow.seek CC M&u(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &ӷ ."WCR"\Forearm.seek CC M&' +46 h '  ."MFBPCR"delay 3 seconds +45 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'{( +105 ."MFBPCR"\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +289 h ( A(\ & h & A( & h & M&z& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&'\ +11 F*|% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +80 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &q( +142 h ( M&& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &l1 +150 h 1 M&% h % A(\\& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&~'B +21 h 'B M&L& h & A(g& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(*% h % ."downElevator"\SE.seek :down A('X +9 h '\ 48 ."MFBPCR"delay 3 seconds +91 A(m% h %B M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &3'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast #\Starting finalPurge A(5 #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M& 1 + A(6 + h 1 M&& h & M&1% h %\  .t\Elbow.seek FlushPuck::Garage M&2(- +66 ."MFBPCR"\ATV.dial :bleach F*%  +22 h F*(% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*p' +78 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &b& h &- .t\Forearm.seek :garage M&s'η +91 h 'η .tHand.close M&'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'i% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CTV C'M  .t\CTV.dial CSV C'R  +37 h  +113  .t\CBV.dial :waste1 C'! @ +76 h @ .t\CS.seek 0ml C'8'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'.& h &W C'Q% h %4 .t\CSV.dial :flush C'V  +75 h  .t\CS.seek 5ml C'Y'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'4 @ +38 h @ C'[% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  +37 h  .t\CBV.dial :waste2b C'K  +76 h  .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'U  +37 h  C'O% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'X'P +1116 h 'P .tdelay 5 seconds +304 h 'V ."MFBPCR"delay 1:00 +196 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'c' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +37 h  .t\CBV.dial :waste1 C'_ @ +76 h @ .t\CS.seek 0ml C'f'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'9  +38 h  C'a% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'k'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste2b C't  +75 h  .t\CS.seek 0ml C'N'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'j  +38 h  C'^% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'o'P +623 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +287 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'T' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r '  +38 h +75 .t\CTV.dial :air C'd  +150 h .t\CS.seek 0ml C'B'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C']'0 +507 h '0 .t\CSV.dial :waste C'v +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'x& h &W C'P% h %4 .t\CSV.dial :flush C'C  +38 h  .t\CS.seek 5ml C'}'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +37 h @ C'% h %p .t\CS.seek 7ml C'H'i +119 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +91 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'; +113 h .t\CTV.dial :air .\CS.seek 0ml C'\ '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +507 h '0 .t\CSV.dial :waste C'z +38 h .t\CS.seek 0ml C''4 +506 h '4 C'|& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'u% h %0 .t\CS.seek 5ml C'l'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\\'0 +507 h '0 .t\CSV.dial :waste C'L +75 h .t\CS.seek 0ml C'g'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'*& h &W C'% h %4 .t\CSV.dial :flush C'q  +37 h  .t\CS.seek 5ml C'm'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '1  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'~'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'n& h &W C'%% h %4 .t\CSV.dial :flush C'(  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C'I'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&<& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &$(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &3& h &ӷ .t\Forearm.seek CC M&s' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'E( +416 h ( M&5& h &ӷ M&2% h % .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &?(- +264 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&A1 +150 h 1 M&)% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &7'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&01 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &&(- +7 h (- M&e& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&:/ +150 h / M&'& h &- M&/% h %η .t\Forearm.seek :retract M&J'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &i' +91 h ' .t\CC.seek :closed C'>(W +416 h (W C'M& h &W .tHand.open M&R1 +150 h 1 M&!% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'.& h &W C'Q% h %4 .t\CSV.dial CSR C'V  +37 h  C'8% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '4'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '[% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'Y'[ +463 h '[ .t\CSV.dial :waste C'` +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'K'4 +2009 h '4 #t\Purging external Intake C'& h &W C'U% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'W' +910 h ' .tdelay 5 seconds +124 h 'V ."MFBPCR"delay 20 seconds +376 ."connect"\CSV.dial CBV C'X .t\CBV.dial CSV C'h  +37 h +38 .t\CTV.dial :intake C'_  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'c'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'9  +75 h #t\Flushing upper manifold C'a& h &W C'f% h %4 .t\CSV.dial :flush C'k  +75 h  .t\CS.seek 5ml C'G'p +401 ."MFBPCR"\ATV.dial :air3 F*t % +107 h % F*6% h %V ."MFBPCR"\AS.seek 0.59ml F*j' +142 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +38 h @ C'N% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'o +113 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +507 h '0 .t\CSV.dial :waste C'd +37 h .t\CS.seek 0ml C'T'4 +507 h '4 C'B& h &W C'v% h %4 .t\CSV.dial :flush C']  +37 h  .t\CS.seek 3ml C'x'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'P @ +38 h @ C'C% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'S'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'} +76 h .t\CS.seek 0ml C';'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &H(ӷ +7 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.seek CC M& ' +91 h ' .tHand.close M&|/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&@'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&L1 +150 h 1 M&\\% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun_postDeploy2 until completion of MFBPCR +2874 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +107 h n ."MFBPCR"\ASV.dial :wasteR F*q% h %  ."MFBPCR"\AS.seek 0.09ml F*g' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*m% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*1 % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*%% h % ."MFBPCR"\AS.seek 0.23ml F*(' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*b'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*I n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*<  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*, I +86 h I ."MFBPCR"\AS.seek 0ml F*5'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*2'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*s  +43 h  #"MFBPCR"\Swish #1 of 2 F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *{'\  +1031 h '\  F*$% h %\  ."MFBPCR"\AS.seek 0.5ml F*A'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*)% h %(A ."MFBPCR"\AS.seek 0.6ml F*+'\  +1032 h '\  F*D% h %\  ."MFBPCR"\AS.seek 0.5ml F*0'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *e' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3472 h 'V ."MFBPCR"\SPE.coast F*:  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h200ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*'  +65 h  ."MFBPCR"\ARV.dial :carrier F*/  +10 h  F*?% h %V ."MFBPCR"\AS.seek 0.5ml F*7'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*J  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *R'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*!  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*.% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*V  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*i' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*[% h % ."MFBPCR"\AS.seek 0.536ml F*4'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*`  +107 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*U n +21 h n ."MFBPCR"delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.6ml F*K'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*h  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.65ml F*_' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*W  +43 h  ."MFBPCR"\ASV.dial :waste1 F*9  +64 h ."MFBPCR"\ARV.dial :air1 F*a  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*f% h % \\ ."MFBPCR"\AS.seek 0.326ml F*k'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi$; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h200ml.pcr F*6% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*^  +21 h ."MFBPCR"\ATV.dial :air3 F*N % +87 h % F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +21 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*T% h %n ."MFBPCR"\AS.seek 0.82ml F*P'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*C  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*x  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *S'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*F  +32 h  ."MFBPCR"\ATV.dial :air3 F*= % +86 h % F*>% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +21 h % ."MFBPCR"delay 3 seconds +300 F*H% h %> ."MFBPCR"\AS.seek 0.835ml F*L'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\\  +21 h F*@% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0105h200ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*~  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*('(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *b'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*5  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*p' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*2  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*$% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +108 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*D'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*+ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*'  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*/  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0105h200ml.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *&'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F*R'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*8% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*[% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Y  +64 h  F*4% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *U'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*9  +32 h  ."MFBPCR"\ATV.dial :air3 F*a % +86 h % F*f% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *_' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*t% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h200ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*P  +43 h  ."MFBPCR"\ARV.dial :carrier F*S  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*=  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*>  +21 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*l' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*H I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*@ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*q% h %< ."MFBPCR"\AS.seek 0.594ml F*j'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*m'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*g  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*n  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*~'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h200ml.pcr F*(% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*I% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*5'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*"% h %n ."MFBPCR"\AS.seek 0.82ml F*2'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*p  +64 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*0  +32 h  ."MFBPCR"\ATV.dial :air3 F*' % +86 h % F*/% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*7% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*E% h %m ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *!'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Y'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h200ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*4  +43 h  ."MFBPCR"\ARV.dial :carrier F*i  +10 h  F*U% h %V ."MFBPCR"\AS.seek 0.5ml F*O'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*h n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*9  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*a  +22 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*c I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*6 n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*N  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*^'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*o n +21 h n ."MFBPCR"delay 2 seconds +200 F*r% h %\  ."MFBPCR"\AS.seek 0.6ml F*B'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*]% h %\  ."MFBPCR"\AS.seek 0.65ml F*T' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*v  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F*P'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h200ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*\  +22 h ."MFBPCR"\ATV.dial :air3 F*y % +87 h % F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*>  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*F'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*@  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*L  +65 h  F*\\% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +31 h  ."MFBPCR"\ATV.dial :air3 F*m % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*~'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*<'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*I' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0112h200ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*)  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*{'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*0  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*'  +22 h ."MFBPCR"delay 2 seconds +200 F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *e'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*: I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*J  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*M% h %< ."MFBPCR"\AS.seek 0.594ml F*!'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*. n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*V'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*8  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*4'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*U % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0112h200ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*Y @@T ."MFBPCR"\ASV.dial :SPEdelivery F*O  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *9' +424 h ' ."MFBPCR"\ATV.dial :ASV F*a n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*f  +43 h  F*K% h % ."MFBPCR"\AS.seek 0.62ml F*c'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*t n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *6'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*k'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*_ % +43 h % F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *G' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*^% h % ."MFBPCR"\AS.seek 0.71ml F*o' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*d' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*B% h % Ű ."MFBPCR"\AS.seek 0.72ml F*v' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*C n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*x% h % zG ."MFBPCR"\AS.seek 0.365ml F*T' +7162 h ' ."MFBPCR"\ATV.dial :waste F*S n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*P% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*z% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %  ."MFBPCR"\AS.seek 0.604ml F*='\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*F' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*H n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *L'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*\\ % +107 h % F*l% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F* ' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h % q ."MFBPCR"\AS.seek 0.605ml F*1'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*g' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F*n'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *('V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*b n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *~'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +202 h 'R ."MFBPCR"\SPE.coast F*3  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*I'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*,  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*s I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*$  +43 h  F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *2': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*A  +65 h  F*p% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *{'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +64 h n F*+% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *D' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*0 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *7': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*e  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b * 'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*? n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *E' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*M  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*. n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *&'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*! n +151 h n ."MFBPCR"\AS.seek 0ml F*8'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*R  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*V % +43 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *Q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*i'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %0 ."MFBPCR"\AS.seek 0.985ml F*4' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*X% h % ."MFBPCR"\AS.seek 0.97ml F*h'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*a I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *K'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *t'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *W'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*6  +43 h  F*_% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *k' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +42 h I ."MFBPCR"\AS.seek 0.02ml F*G' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*^'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*o % +43 h % F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *B'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*C% h %Z ."MFBPCR"\AS.seek 0.81ml F*x'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +21 h % ."MFBPCR"delay 3 seconds +300 F*S% h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*P% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*z  +65 h  F*\ % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *>'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*F % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*l% h % ."MFBPCR"\AS.seek 0.81ml F*L'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*q % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*j% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*1' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *~'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*< % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun_postDeploy2 assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:41:16, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy2 assays" #\Mission runtime was 2:05.976644 #-> exit #m\EXIT @1542782561.40CET @1542782789.93CET = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy3.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy3 w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:46:37, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy3 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tun_postDeploy3.rb:103\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:46:59, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:47:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy3 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 4 at 07:47:03CET21-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:47:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*/% h %&K #tLoading sh1 puck from tube 4 into Collection Clamp #"readyMFB"Readying the MFB C'0& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil F* .tHand.open M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&I& A(H'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'*9 h 9 d +100 C'9 h 9 *d +100 C'+9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'H9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'89 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +423 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +37 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'4 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'` +113 h .t\CBV.dial CSV C'a  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +38 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +131 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (i( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b M&^& A(o& h &ӷ & .t\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit .t\Elbow.seek CC A(q'Z M&$&.,(\ #b &r(ӷ +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(n& h & A(y& h & M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&& h & A({& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +18 h @ .t\CS.seek 10ml C'' +6 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%$  C'9  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%<  +113 h .t\PS.seek 0ml P%=' +2610 h ' .t\PSV.dial :air P%?  +37 h  .t\PS.seek 2.5ml P%:' +2610 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%B +37 h .t\PS.seek 0ml P%I' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +265 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'V& h & M&R% h %\  A(8% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (T(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &,9 M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &\\(w +166 h (w M&W& h &w A(4& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &Z' +89 h ' A(`& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(]& h &,9 M&_& h &w .t\Elbow.seek 4 M&d(w +7 h (w M&e& h &w A(c& h &,9 .t\Forearm.seek 4 + 300 counts M&g'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &X/ +150 h / ."downElevator"\SE.seek :down M&f& A(j'X h &w M&k% h %7 .t\Forearm.seek :retract M&l'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +142 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +151 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%{ +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +150 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +142 h ( M&& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h & .t\SE.seek -0.29 Pucks below Top Plate A('\\ +265 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &"' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&+% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&('B +21 h 'B M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +36 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +90 h '\  .t\Elbow.seek CC M&5(ӷ +142 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'9( +416 h ( A(0& h & A(<& h & M&& h &ӷ M&?% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &A( +142 h ( M&:& h & A(E& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &D' +93 h ' .t\Elbow.hold M&B, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h % A(&& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &J'B +21 h 'B M&>& h & A(*& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  +2 A(6 +111 h  P%G% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +37 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'R  +37 h .t\PRV.dial between CBV and air P%8  +19 h  .t\PRV.dial :air P%Y  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&41 +2 h 1 M&`& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&X& h &- M&f% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&j1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +90 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%o' +1598 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 0ml P%n' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +37 h  .tdelay 2 seconds +200 P%I% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +75 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1313 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '+& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*w @@T .t\AIV.dial :bypass F*  .t\PSV.dial PSR P%2 +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%i' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*$  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*? % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%6' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F** I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2609 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%K' +739 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%.' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +113 h P%8% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Y' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +37 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %`'k +2678 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +21 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +849 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%X' +2610 h ' .t\PSV.dial :mixloop P%f +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %H' +5107 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1026 h 'Q ."MFBPCR"\ATV.dial :AIV F*9 I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*j' +171 h ' .t\PSV.dial :mixloop P%d +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*^'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*o% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'I' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  'u  +38 h  +113  .t\CBV.dial :waste1 C'r @ +37 h @ .t\CS.seek 0ml C'{'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +109 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 4 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (i(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &,9 M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&$& h &w A(s& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(<& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &,9 M&?& h &w ."WCR"\Elbow.seek 4 M&0(w +7 h (w M&E& h &w A(5& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&)'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&&& A(:'X h &w M&*% h %7 ."WCR"\Forearm.seek :retract M&'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +142 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +29 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 1500ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +7 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +22 h F*i% h %X ."MFBPCR"\AS.seek 0.24ml F*=' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*R n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*8  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *Y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*. @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*4'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*['(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*@  +43 h  #"MFBPCR"\Swish #1 of 2 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *e'\  +1032 h '\  F*W% h %\  ."MFBPCR"\AS.seek 0.5ml F*U'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*O% h %(A ."MFBPCR"\AS.seek 0.6ml F*X'\  +1032 h '\  F*c% h %\  ."MFBPCR"\AS.seek 0.5ml F*H'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*h% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *_' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*f % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +3473 h 'V ."MFBPCR"\SPE.coast F*9  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0104h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*a  +64 h  ."MFBPCR"\ARV.dial :carrier F*d  +10 h  F*k% h %V ."MFBPCR"\AS.seek 0.5ml F*]'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*j  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *^'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*P  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*}  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*r % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +226038 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'~  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'b' +909 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'\\ @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'g  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'l ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'+  'm  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'2  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'3  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'p' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)1% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'% ` +57 h ` ."WCR"\CS.seek 7.288ml C's' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'<  '$  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'5  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'E% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'*  +94 h  C'A% h % ."WCR"\CSV.dial between air and CTV C') ` +57 h ` ."WCR"\CS.seek 7.288ml C'7' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'D  ':  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'"  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&J% h %\  A(/% h %X A('. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (K( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h & M&& h &ӷ M&M% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &i( +142 h ( M&>& h & A(R& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &8' +90 h ' A(=& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(!'K +253 h 'K M&V& h & A(Y& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M&Q, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(4- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(@' +27 h ' M&`% h % A([& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &W'B +21 h 'B M&e& h & A(O& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &U' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(c' +36 h ' M&X& h & M&h% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +90 h '\  ."WCR"\Elbow.seek CC M&f(ӷ +142 h (ӷ M&_& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ ."WCR"\Forearm.seek CC M&a' +91 h ' ."WCR"Hand.close M&d/ +150 h / ."WCR"\CC.seek :open C'k( +416 h ( A(B& h & A(]& h & M&N& h &ӷ M&t% h % ."WCR"\Forearm.seek :retract M&v'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &j( +143 h ( M&C& h & A(o& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &S' +92 h ' ."WCR"\Elbow.hold M&P, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &y1 +150 h 1 M&^% h % A(}& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&n'B +21 h 'B M&T& h & A(r& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &I' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (x% h %B M&u% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&1 +2 A(6 h 1 M&\ & h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&z& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'g& h &W .tHand.open M&L1 +150 h 1 M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'+& h &W C'm% h % .t\CSV.dial CSR C'  +38 h  C'2% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'p& h &W C' % h %4 .t\CSV.dial :flush C'1  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'%% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '<  +38 h  +113  .t\CBV.dial :waste1 C's @ +75 h @ .t\CS.seek 0ml C'?'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'5% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'E'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C',  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'*  +75 h  .t\CS.seek 0ml C'A'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C')  +38 h  C'&% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ':'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +710 h ' ."connect"\CSV.dial CTV C'7  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'" @ +75 h @ .t\CS.seek 0ml C'0'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C''  +37 h  .t\CBV.dial :waste2b C'F  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'M  +38 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'R'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6  'G +38 h +75 .t\CTV.dial :air C'V  +151 h .t\CS.seek 0ml C'Y'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +507 h '0 .t\CSV.dial :waste C'` +38 h .t\CS.seek 0ml C'['4 +506 h '4 #t\Flushing upper manifold C'e& h &W C'O% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'i% h %p .t\CS.seek 7ml C'!'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'_'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'@'4 +506 h '4 C'U& h &W C'Q% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'H'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +37 h @ C'B% h %0 .t\CS.seek 5ml C']'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'N'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'C +75 h .t\CS.seek 0ml C'o'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'f% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 5ml C'^'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +37 h @ C'T% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'v  +38 h  +113  .t\CBV.dial :waste1 C'j @ +38 h @ .t\CS.seek 0ml C'x'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'u& h &W C'{% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'S% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV C'c  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'I  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&g1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'+& h & .tHand.open M&m1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &2(- +7 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&3% h %η .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'%(W +416 h (W C'& h &W .tHand.open M&$1 +150 h 1 M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'% h %4 .t\CSV.dial CSR C'5  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '?'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'E +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2010 h '4 #t\Purging external Intake C'*& h &W C'% h %4 .t\CSV.dial :flush C')  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C': 'A  +38 h +37 .t\CTV.dial :intake C'7  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'"'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'D  +75 h #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'0  +75 h  .t\CS.seek 5ml C'J'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +37 h @ C'F% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV C'M .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'>'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'R +37 h .t\CS.seek 0ml C'6'4 +507 h '4 C'G& h &W C'V% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.seek 3ml C'Y'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'O'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +507 h '0 .t\CSV.dial :waste C'[ +75 h .t\CS.seek 0ml C'8'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'9( +416 h ( M&U& h &ӷ M&Q% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &H'η +91 h 'η .tHand.open M&]1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun_postDeploy3 until completion of MFBPCR +285465 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h1500ml.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*f  +21 h ."MFBPCR"\ATV.dial :air3 F*y % +87 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *}'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*x  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*z  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*L% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*+  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *m'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F*p'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*?  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F**  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*,  +107 h  ."MFBPCR"delay 7 seconds +700 F*A% h %< ."MFBPCR"\AS.seek 0.594ml F*:'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*7 n +21 h n ."MFBPCR"delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*'  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*/ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h1500ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*G % +86 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +21 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*4'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*O'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +65 h  F*W% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +87 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*H% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*f'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*o% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*T  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0108h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*I'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*~  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +21 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*g' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*L % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*+'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*m% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*l  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*1% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*2  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0108h1500ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*|  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +86 h % F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*,% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *A'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*D  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*G% h %m ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*R  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0111h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*i  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*U n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +21 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *;'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*d I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*H% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*t n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*w' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*y  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.536ml F*f'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +108 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*^'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*} n +22 h n ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*c % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0111h1500ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*P% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*g% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*m' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*2'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*,  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +10 h  F*A% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*D  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*0 I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*. % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*R  +21 h  ."MFBPCR"delay 7 seconds +700 F*V% h %< ."MFBPCR"\AS.seek 0.594ml F*6'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*` n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*4% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*O  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*! @@T ."MFBPCR"\ASV.dial :SPEdelivery F*s  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*%  +150 h F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *B' +424 h ' ."MFBPCR"\ATV.dial :ASV F*h n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*_% h % ."MFBPCR"\AS.seek 0.62ml F*d'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*H n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *;'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *t'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *o' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*f% h % ."MFBPCR"\AS.seek 0.71ml F*}' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*j% h % Ű ."MFBPCR"\AS.seek 0.72ml F*r' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +85 h n ."MFBPCR"\ASV.dial :SPErecovery F* % h % zG ."MFBPCR"\AS.seek 0.365ml F*{' +7162 h ' ."MFBPCR"\ATV.dial :waste F*\ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +21 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*I' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %  ."MFBPCR"\AS.seek 0.604ml F*~'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*g % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +21 h F*+% h % ."MFBPCR"\AS.seek 0.58ml F*\\' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*1 n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*3  +21 h  ."MFBPCR"\AS.seek 0ml F*l'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*2  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *|' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +202 h 'R ."MFBPCR"\SPE.coast F*5  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*<'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*E I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b **': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*A  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *)'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*& n +64 h n F*7% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *:' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*D I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*.% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *>'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*F n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *G' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*/ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*V  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*` n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *='Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*6 n +151 h n ."MFBPCR"\AS.seek 0ml F*e'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*4  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*Y % +43 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h % ."MFBPCR"\AS.seek 0.96ml F*i'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %0 ."MFBPCR"\AS.seek 0.985ml F*W' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*Q% h % ."MFBPCR"\AS.seek 0.97ml F*U'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*h I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *H'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *;'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*t  +43 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *q' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +43 h I ."MFBPCR"\AS.seek 0.02ml F*o' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +128 h n ."MFBPCR"\AS.seek 0ml F*f'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*T% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*^  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +43 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *j'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*( % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2548 h 'V #t\Asleep after completing 18tun_postDeploy3 assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@07:47:50, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy3 assays" #\Mission runtime was 2:07.28435 #-> exit #m\EXIT @1542783490.56CET @1542797253.84CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy2.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy2 w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:47:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy2 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tun_postDeploy2.rb:103\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:07, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy2 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 150ml, wcr at most 150ml .BACfans.suppressMail "Started mfb in tube 4 at 11:48:07CET21-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:07, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 150ml, wcr at most 150ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 150ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +150 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'+9 h 9 d +100 C'9 h 9 *d +100 C'*9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'.9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'89 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +113 h @ .t\CSV.dial :air C'! @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +37 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'4 @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'` +113 h .t\CBV.dial CSV C'a  +18 h C']% h %4 .t\CS.seek 5ml C'c'p +1373 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +37 h C'e% h %p .t\CS.seek 7ml C'f'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +37 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +132 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (i( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (o& h & M&^& ."raiseStack"\SE.seek :upperLimit h &ӷ A(q'Z .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &r(ӷ +7 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(n& h & A(y& h & M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +142 h ( M&& h & A({& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'B +20 h 'B M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~' +23 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C' { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +150 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV P%$  .t\CBV.dial PRV C'9  h +76 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%<  +113 h .t\PS.seek 0ml P%=' +2609 h ' .t\PSV.dial :air P%?  +38 h  .t\PS.seek 2.5ml P%:' +2610 h ' .t\PSV.dial :mixloop P%A +37 h .t\PS.seek 0ml P%D' +2610 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2609 h ' .t\PSV.dial :mixloop P%B +38 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +6 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&.% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'V& h & M&R% h %\  A(8% h %X A(X. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (T(,9 +201 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (!& h &,9 M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &\\(w +165 h (w M&W& h &w A(4& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &Z' +90 h ' A(`& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(]& h &,9 M&_& h &w .t\Elbow.seek 4 M&d(w +6 h (w M&e& h &w A(c& h &,9 .t\Forearm.seek 4 + 300 counts M&g'7 +23 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &Y/ +150 h / ."downElevator"\SE.seek :down A(f'X M&j& h &w M&k% h %7 .t\Forearm.seek :retract M&l'\ +65 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +143 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +150 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2609 h ' .t\CBV.dial :waste1 C' @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C' ' +2648 h ' .t\CSV.dial CTV C'  +37 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h & .t\SE.seek -0.29 Pucks below Top Plate A('\\ +264 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 3 M&$ ,(#b &#Q@2^d^2b &"' +26 h ' .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&*% h % A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &('B +20 h 'B M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +37 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'9( +415 h ( A(0& h & A(<& h & M&& h &ӷ M&?% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &A( +143 h ( M&:& h & A(E& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &D' +92 h ' .t\Elbow.hold M&B, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h % A(&& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&J'B +20 h 'B M&>& h & A(+& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  + A(6 +112 h  P%G% h % .t\PS.seek 0.499ml P%Q' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%. +38 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +37 h .t\CBV.dial AIV C'R  +38 h .t\PRV.dial between CBV and air P%8  +19 h  .t\PRV.dial :air P%X  +19 h  P%!% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&41 +2 h 1 M&`& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&Y& h &- M&j% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +264 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +91 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&f1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%n' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +76 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .t\PS.seek 0ml P%z' +2609 h ' .t\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P% ' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1313 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '*& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& h &W F*w @@T ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*  P%2 +21 h  ."MFBPCR"delay 20 seconds +92 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%i' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +38 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*$  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*? % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +37 h  .t\PS.seek 0ml P%6' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F*+ I +42 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2610 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +38 h  .t\PS.seek 0ml P%K' +739 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%.' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +112 h P%8% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%X' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%!  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %`'k +2679 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +850 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%Y' +2609 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5106 h ' .t\PSV.dial :air P%c  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +43 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*9 I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*f' +171 h ' .t\PSV.dial :mixloop P%d +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +619 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*o'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*^% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'H' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  'u  +37 h  +113  .t\CBV.dial :waste1 C'r @ +38 h @ .t\CS.seek 0ml C'{'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +22 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'q' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +7 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&*& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 150ml #Loading wcr puck from tube 4 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (i(,9 +200 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &,9 M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&$& h &w A(s& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &,' +90 h ' A(<& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &,9 M&?& h &w ."WCR"\Elbow.seek 4 M&0(w +6 h (w M&E& h &w A(5& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&)'7 +22 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&&& A(:'X h &w M&+% h %7 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +143 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +30 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 150ml w/bubble point of 55psi #\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'M  +150 h C'i& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&=% h %\  A(8% h %X A(R. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (V( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (!& h & M&X& h &ӷ M&.% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &Q( +142 h ( M&& h & A(4& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &[' +90 h ' A(@& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(`'K +253 h 'K M&e& h & A(W& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &U' +25 h ' ."WCR"\Elbow.hold M&O, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(Y- h -+K M&$&.,(\ #b ."WCR"\SE.seek 1.04 Pucks below Top Plate A(c' +27 h ' M&I% h % A(h& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&_'B +21 h 'B M&j& h & A(B& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &9' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(a' +36 h ' M&d& h & M&k% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &]'\ +90 h '\  ."WCR"\Elbow.seek CC M&t(ӷ +142 h (ӷ M&N& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ ."WCR"\Forearm.seek CC M&S' +91 h ' ."WCR"Hand.close M&f/ +89 h ' q ."MFBPCR"delay 3 seconds +61 h / ."WCR"\CC.seek :open C'C( +239 ."MFBPCR"\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +155 h ( A(o& h & A(P& h & M&y& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&T'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &n( +54 F*u% h % q ."MFBPCR"\AS.seek 0.794ml F*}' k +89 h ( M&H& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &r' +92 h ' ."WCR"\Elbow.hold M&x, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A({& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts + M&\ 'B +21 h 'B M& & h & A(z& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(|'X +100 (% h %B M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +43 h ' k ."MFBPCR"delay 3 seconds +46 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'q& h & .tHand.open M&1 +2 A(6 h 1 M&~& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&b(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &l& h &- .t\Forearm.seek :garage M&g'η +34 ."MFBPCR"\ATV.dial :bleach F*L  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +35 h 'η .tHand.close M&\\/ +150 h / M&m& h &- M& % h %η .t\Forearm.seek :retract M&2'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &3(ӷ +48 h '  ."MFBPCR"delay 3 seconds +216 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +84 ."MFBPCR"\ATV.dial :AIV F*p I +7 h ' .t\CC.seek :closed C'1(W +100 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +316 h (W C'%& h &W .tHand.open M&*1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &('\ +91 h '\  #t\Flushing the Collection Valve::Manifold C's& h &W C'<% h % .t\CSV.dial CSR C'  +38 h  C'$% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'E% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '5'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +1323 h ' ."MFBPCR"\ATV.dial :waste F*& n +129 h n ."MFBPCR"\AS.seek :empty F*?'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*,% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +177 h '4 #t\Flushing lower manifold's waste1 port C'A& h &W C'% h %4 .t\CSV.dial :flush C'"  +23 ."MFBPCR"\ATV.dial :bleach F*D  +14 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ')'p +8 h F*0% h % ."MFBPCR"\AS.seek 0.579ml F*' +643 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C': @ +37 h @ C' % h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'/  +38 h  +113  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C'J'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'F& h &W C'7% h %4 .t\CSV.dial :flush C'M  +75 h  .t\CS.seek 5ml C'i'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'= @ +37 h @ C'8% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV C'!  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'X  +75 h  .t\CS.seek 0ml C'.'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C'4% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '@'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '6' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 C'e @ +75 h @ .t\CS.seek 0ml C'W'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'Y  +37 h  C'I% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'h'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +710 h ' ."connect"\CSV.dial CTV C'B  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'd  +76 h  .t\CS.seek 0ml C'k'4 +451 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*[ % +21 h % ."MFBPCR"delay 3 seconds +137 h '4 #tKilling upper manifold .\CSV.dial CSR C'Q  +38 h  C'`% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'U'P +119 F*N% h %  ."MFBPCR"\AS.seek 0.604ml F*v'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F*c% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*9' +150 h 'P .tdelay 5 seconds +174 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*O n +26 .t\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +53 h n ."MFBPCR"\ASV.dial :wasteR F*]  +43 h  ."MFBPCR"\AS.seek 0ml F*^'V +613 h ' ."connect"\CSV.dial CBV C'; .t\CBV.dial CSV C'o  +38 h +75 .t\CTV.dial :air C'P  +151 h .t\CS.seek 0ml C'y'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'H'4 +506 h '4 #t\Flushing upper manifold C'& h &W C'S% h %4 .t\CSV.dial :flush C't  +38 h  .t\CS.seek 5ml C'K'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'z'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'T +38 h .t\CS.seek 0ml C'n'4 +506 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +37 h @ C'% h %0 .t\CS.seek 5ml C'r'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'\ '4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'l& h &W C'L% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'V'p +88 h 'V ."MFBPCR"delay 1:00 +562 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +38 h @ C'x% h %p .t\CS.seek 7ml C'\\'i +415 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'm  '  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'}'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'g& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'b'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'p @ +38 h @ C'2% h %p .t\CS.seek 7ml C'C'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'%  +76 h  .t\CS.seek 0ml C'*'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +6 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&$' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'>( +416 h ( M&E& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &5(- +218 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +40 h (- M&&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &,'η +91 h 'η .tHand.open M&1 +69 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +81 h 1 M&A% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'"& h & .tHand.open M&D1 +2 h 1 M&0& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &- .t\Forearm.seek :garage M& 'η +91 h 'η .tHand.close M&)/ +150 h / M& & h &- M&/% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &'(ӷ +264 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &M' +91 h ' .t\CC.seek :closed C'J(W +416 h (W C'=& h &W .tHand.open M&81 +150 h 1 M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'X& h &W C'R% h %4 .t\CSV.dial CSR C'  +38 h  C'4% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '.'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '@% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'G'[ +463 h '[ .t\CSV.dial :waste C'e +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '6'4 +1710 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Y'V +94 h '4 #t\Purging external Intake C'I& h &W C'W% h %4 .t\CSV.dial :flush C'h  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'B' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'd 'j  +38 h +37 .t\CTV.dial :intake C'[  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'Q'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'`  +75 h #t\Flushing upper manifold C'N& h &W C'k% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'_'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +37 h @ C'v% h %p .t\CS.seek 7ml C'O'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'U'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +507 h '0 .t\CSV.dial :waste C'o +37 h .t\CS.seek 0ml C';'4 +507 h '4 C'P& h &W C'f% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'u'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C'S'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C't'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&K& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.seek CC M&T' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&q% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &~(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &r'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &a'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun_postDeploy2 until completion of MFBPCR +7846 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*l % +108 h % F*L% h %V ."MFBPCR"\AS.seek 0.59ml F*' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +108 h n ."MFBPCR"\ASV.dial :wasteR F*|% h %  ."MFBPCR"\AS.seek 0.09ml F*x' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*V  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*m I +86 h I F* % h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*\\ % +21 h % F*g% h % ."MFBPCR"\AS.seek 0.13ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.23ml F*p' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*2 % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h % ."MFBPCR"\AS.seek 0.255ml F*'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*C% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *('5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*1 @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +85 h I ."MFBPCR"\AS.seek 0ml F*$'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*&'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +42 h  #"MFBPCR"\Swish #1 of 2 F*s% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*A% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*"% h %(A ."MFBPCR"\AS.seek 0.6ml F*D'\  +1031 h '\  F*0% h %\  ."MFBPCR"\AS.seek 0.5ml F*+'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*:% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *^' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*, % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h150ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*/  +64 h  ."MFBPCR"\ARV.dial :carrier F*5  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*7 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*>  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +21 h ."MFBPCR"delay 2 seconds +200 F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *i'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*X  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*4  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.53ml F**' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*@ % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*G  +107 h  ."MFBPCR"delay 7 seconds +700 F*I% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*h n +22 h n ."MFBPCR"delay 2 seconds +200 F*?% h %\  ."MFBPCR"\AS.seek 0.6ml F*6'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*j  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*d% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*B  +43 h  ."MFBPCR"\ASV.dial :waste1 F*`  +65 h ."MFBPCR"\ARV.dial :air1 F*N  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*k% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*c % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h150ml.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*]  +22 h ."MFBPCR"\ATV.dial :air3 F*O % +86 h % F*U% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *o'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +21 h F*P% h %Z ."MFBPCR"\AS.seek 0.81ml F*f'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*u% h %n ."MFBPCR"\AS.seek 0.82ml F*S'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*H% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*n% h %> ."MFBPCR"\AS.seek 0.835ml F*r'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*L% h %m ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *|'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0105h150ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*2  +11 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*p'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*C n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*(  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*E' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*$  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*s' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*&  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*"  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*0'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*+'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*,  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*5  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0105h150ml.pcr F*F% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*>  +21 h ."MFBPCR"\ATV.dial :air3 F*= % +87 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*X  +22 h F*R% h %Z ."MFBPCR"\AS.seek 0.81ml F*i'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*4 % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*.% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*G  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*I  +65 h  F*e% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *h'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*?' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*`  +32 h  ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*k% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*c% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*]'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*U% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*9  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*o' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0107h150ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*K  +10 h  F*{% h %V ."MFBPCR"\AS.seek 0.5ml F*z'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*n I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F*l' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*L % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +107 h  ."MFBPCR"delay 7 seconds +700 F*|% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*V n +22 h n ."MFBPCR"delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*\\% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*2'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*b  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0107h150ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*%  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*3  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*('> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*E% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*A  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*s  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +31 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F*5% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +21 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +21 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*8'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h150ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*e  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +10 h  F*h% h %V ."MFBPCR"\AS.seek 0.5ml F*?'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*j n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *d'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*`  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*N  +22 h ."MFBPCR"delay 2 seconds +200 F*k% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *B'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*Q I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*c% h %Fj ."MFBPCR"\AS.seek 0.524ml F*[' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*v n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*O  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.536ml F*]'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*9  +107 h  ."MFBPCR"delay 7 seconds +700 F*_% h %< ."MFBPCR"\AS.seek 0.594ml F*P'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*o n +21 h n ."MFBPCR"delay 2 seconds +200 F*y% h %\  ."MFBPCR"\AS.seek 0.6ml F*;'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*f  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*u' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*{ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h150ml.pcr F*H% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*J% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*\ % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*r  +64 h  F*l% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\\  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*g% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*2'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *3'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *s'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0111h150ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*"  +43 h  ."MFBPCR"\ARV.dial :carrier F*&  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *,'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*/  +21 h ."MFBPCR"delay 2 seconds +200 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *^'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*1 % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.53ml F*7' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*>  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*8% h %< ."MFBPCR"\AS.seek 0.594ml F*X'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*M  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*i% h %\  ."MFBPCR"\AS.seek 0.65ml F*@' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*.  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*e'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*h % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0111h150ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*I @@T ."MFBPCR"\ASV.dial :SPEdelivery F*?  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*W  +151 h F*d% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *`' +423 h ' ."MFBPCR"\ATV.dial :ASV F*N n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*k  +43 h  F*6% h % ."MFBPCR"\AS.seek 0.62ml F*Q'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*c n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *v'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*[ % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *U' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*]% h % ."MFBPCR"\AS.seek 0.71ml F*o' H +3640 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h % H ."MFBPCR"\AS.seek 0.735ml F*f' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*;% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*t n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*u' +7161 h ' ."MFBPCR"\ATV.dial :waste F*K n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *{'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*H % +43 h % F*S% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*!% h % ."MFBPCR"\AS.seek 0.579ml F*z' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +21 h % ."MFBPCR"delay 3 seconds +300 F*J% h %  ."MFBPCR"\AS.seek 0.604ml F*q'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*n n +86 h n ."MFBPCR"\ASV.dial :wasteR F*\  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*l % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*|  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*V' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*\\'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*x' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*g  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*b  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *%' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *2'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*C n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*3  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*<'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *(' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *E'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*A  +42 h  F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *&': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*s% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*: n +65 h n F*D% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *0' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b */'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*,  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b * ': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*^  +64 h  F*1% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *F'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*>% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *=' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*7 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*8  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *)': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*R n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*X n +150 h n ."MFBPCR"\AS.seek 0ml F*M'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*i  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*4 % +43 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*@% h % ."MFBPCR"\AS.seek 0.96ml F**'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %0 ."MFBPCR"\AS.seek 0.985ml F*e' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*d% h % ."MFBPCR"\AS.seek 0.97ml F*j'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*N I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *6'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *c'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *B'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*v  +43 h  F*[% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +43 h I ."MFBPCR"\AS.seek 0.02ml F*U' +9880 h ' ."MFBPCR"\ATV.dial :waste F*_ n +129 h n ."MFBPCR"\AS.seek 0ml F*]'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*9% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*P  +21 h ."MFBPCR"\ATV.dial :air3 F*o % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *;'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*K% h %> ."MFBPCR"\AS.seek 0.835ml F*u'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*S% h %n ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*!  +64 h  F*'% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *T'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*r'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*3 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *2'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun_postDeploy2 assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:48:55, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy2 assays" #\Mission runtime was 2:07.439471 #-> exit #m\EXIT @1542797543.86CET @1543072010.27CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '18tun_postDeploy3.rb' .GoodNews.suppressMail "Starting 18tun_postDeploy3 w/pucks in tube 4\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:06:58, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun_postDeploy3 begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 4: mbaPhase\\n/home/esp/esp2/mission/18tun_postDeploy3.rb:103\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:07:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:07:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun_postDeploy3 phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== #\Starting mfb #pcrBAC sampling at most 1200ml, wcr at most 1200ml .BACfans.suppressMail "Started mfb in tube 4 at 16:07:23CET24-Nov-18\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:07:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1200ml, wcr at most 1200ml" F*/% #tLoading sh1 puck from tube 4 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil F* M&11 +6 F*2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (8(,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &,9 M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &;(w +19 h (w M&<& h &w A(=& h &,9 .t\Forearm.seek 4 + 700 counts M&>' +90 h ' A(?& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &,9 M&B& h &w .t\Elbow.seek 4 M&C(w +7 h (w M&D& h &w A(E& h &,9 .t\Forearm.seek 4 + 300 counts M&F'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& A(I'X h &w M&J% h %7 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 1200ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X +151 h .t\SS.reconfigure SSconfig .\SS.seek 0ml S)#.0Xd +100 C'9 h 9Td +100 C'{9 h 9kd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9 d +100 C'x9 h 9 d +100 C'~9 h 96d +100 C'\ 9 h 9Ld +100 C' 9 h 9bd +100 C' 9 h 9xd +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9(d +100 C'9 h 9>d +100 C'9 h 9Td +100 C'9 h 9id +100 C' 9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'9 h 9d +100 C'"9 h 9d +100 C'(9 h 9  d +100 C'*9 h 9 d +100 C'9 h 9 *d +100 C'+9 h 9 ?d +100 C',9 h 9 Td +100 C'9 h 9 j d +100 C'9 h 9 $d +100 C'9 h 9 (d +100 C''9 h 9 ,d +100 C'/9 h 9 0d +100 C'29 h 9 4d +100 C'39 h 9 8d +100 C'19 h 9 9 h 9 d +100 C'#9 h 9 d +100 C'N9 h 9 d +100 C'M9 h 9 d +100 C'F9 h 9 d +100 C'@9 h 9 1d +100 C'I9 h 9 Dd +100 C'K9 h 9 Xd +100 C'C9 h 9 kd +100 C'Q9 h 9 ~d +100 C'-9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9 d .t\CH.hold 5:00,85C C'R   P h  +30000  C'!9 h 9  #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C'O'4 +423 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'T @ +112 h @ .t\CSV.dial :air C'8 @ +38 h @ .t\CS.seek 5ml C'Z'p +1372 h 'p .t\CSV.dial CTV C'[  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C'\\ @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'W  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'4 @ +38 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'_'4 +1372 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'` +113 h .t\CBV.dial CSV C'a  +19 h C']% h %4 .t\CS.seek 5ml C'c'p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'd  +38 h C'e% h %p .t\CS.seek 7ml C'f'i +606 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'g @ +38 h @ .t\CS.seek 10ml C'b' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'j ` +131 h ` #tLysing Complete C'k& h &W #t\Storing sh1 puck in Collection Clamp to tube 3 M&l% h %\  A(m% h %X A(h. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (i( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (o& M&^& h & &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(q'Z .t\Elbow.seek CC M&$&.,(\ #b &r(ӷ +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&v/ +150 h / .t\CC.seek :open C'w( +23 h 'Z +393 ( A(n& h & A(y& h & M&z& h &ӷ M&}% h % .t\Forearm.seek :retract M&|'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&& h & A({& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +92 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&'B +21 h 'B M&& h & A(x& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &~' +22 h ' A(\ % h %Z ."downElevator"\SE.seek :down A( 'X +100 ( % h % M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ + A(6 +17 h @ .t\CS.seek 10ml C'' +7 h ' .t\CSV.dial between air and CTV C' ` +19 h ` .t\AIV.dial :bypass C' { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &,' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&'% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &/'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%2 +151 h P%3% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%1' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%5  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV P%$  .t\CBV.dial PRV C'9  h +75 .t\PSV.dial :mixloop P%0 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%<  +113 h .t\PS.seek 0ml P%=' +2610 h ' .t\PSV.dial :air P%?  +37 h  .t\PS.seek 2.5ml P%:' +2610 h ' .t\PSV.dial :mixloop P%A +38 h .t\PS.seek 0ml P%D' +2609 h ' .t\PSV.dial :air P%E  +38 h  .t\PS.seek 2.5ml P%)' +2610 h ' .t\PSV.dial :mixloop P%B +37 h .t\PS.seek 0ml P%H' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&7& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ .t\Forearm.seek CC M&;' +90 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'#( +416 h ( M&N& h &ӷ M&M% h % .t\Forearm.seek :retract M&F'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +265 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &C'η +91 h 'η .tHand.open M&Q1 +150 h 1 M&-% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'V& h & M&R% h %\  A(!% h %X A(Y. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (T(,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (8& h &,9 M&O& h &- M&[% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &\\(w +166 h (w M&W& h &w A(4& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &Z' +89 h ' A(`& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A(a' +66 h ' A(]& h &,9 M&_& h &w .t\Elbow.seek 4 M&d(w +7 h (w M&e& h &w A(c& h &,9 .t\Forearm.seek 4 + 300 counts M&g'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &X/ +150 h / ."downElevator"\SE.seek :down M&j& A(f'X h &w M&k% h %7 .t\Forearm.seek :retract M&l'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&m(ӷ +142 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C's(W +416 h (W .tHand.open M&t1 +150 h 1 M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'S  +151 h  .t\CSV.dial CTV C'P  +19 h  .t\CS.seek 5ml C'n'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'y' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'z'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%}  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :mixloop P%{ +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%u  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%r +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%  +151 h  .t\PS.seek :empty P%' +2610 h ' .t\CBV.dial :waste1 C' @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'x @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'\  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'| ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C' ' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'q& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M& % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&& h &ӷ M&~% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b & ( +143 h ( M&& h & A( & h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &i' +89 h ' A(b& h & .t\SE.seek -0.29 Pucks below Top Plate A('\\ +265 h '\\ M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &"' +25 h ' .t\Elbow.hold M&, +7 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(' +26 h ' M&+% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&('B +21 h 'B M&w& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A('' +36 h ' M&2& h & M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &,'\ +89 h '\  .t\Elbow.seek CC M&5(ӷ +143 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&6' +91 h ' .tHand.close M&$/ +150 h / .t\CC.seek :open C'9( +416 h ( A(0& h & A(<& h & M&& h &ӷ M&?% h % .t\Forearm.seek :retract M&='\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &A( +142 h ( M&:& h & A(E& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &D' +93 h ' .t\Elbow.hold M&B, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&7% h % A(&& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &J'B +21 h 'B M&>& h & A(*& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &/' +22 h ' A(% h % ."downElevator"\SE.seek :down A(N'X +100 (M% h %S M&;% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%K  +2 A(6 +111 h  P%G% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%- +37 h .t\PRV.dial :air P%F  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%V  +38 h .t\CBV.dial AIV C'R  +37 h .t\PRV.dial between CBV and air P%!  +19 h  .t\PRV.dial :air P%Y  +19 h  P%8% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %O' +250 h ' .t\PRV.dial :waste2 P%[ @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %C' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'W& h & .tHand.open M&41 +2 h 1 M&`& h & M&@% h %\  .t\Elbow.seek FlushPuck::Garage M&](- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&c'η +91 h 'η .tHand.close M&U/ +150 h / M&X& h &- M&j% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\\(ӷ +265 h (ӷ M&Z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &o' +90 h ' .t\CC.seek :closed C'g(W +416 h (W C'a& h &W .tHand.open M&f1 +150 h 1 M&d% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  C'#& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%t @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%^' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%T' +1094 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 0ml P%n' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%y +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%}  +38 h  .tdelay 2 seconds +200 P%H% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %{'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%u  +75 h .tdelay 2 seconds +200 P%r% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%x  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %\ ' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%| +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%z' +2610 h ' .t\PSV.dial :air P%  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P% +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%q  +75 h  .t\PS.seek 2.5ml P% ' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P% +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%  +76 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'~ & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb % 'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'b { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %m' +1313 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%' +2609 h ' .t\PSV.dial :PML P% +38 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '+& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'& F*w @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR F*  P%2 +22 h  ."MFBPCR"delay 20 seconds +91 h P%3% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%i' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F* I +129 h I F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *"'6 +66 .t\PSV.dial :waste1 P%1  +37 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%( +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*$  +22 h ' .t\PSV.dial PSR P%, +21 h  F*s% h %6 ."MFBPCR"\AS.seek 0.329ml F*0'ͯ +92 h P%<% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*? % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%E  +38 h  .t\PS.seek 0ml P%6' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*5  +22 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *7' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%& +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%>' +217 ."MFBPCR"\ATV.dial :AIV F** I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*=' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%A +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%M' +2609 h ' .t\PSV.dial PSR P%; +113 h P%D% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%J' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%'  +37 h  .t\PS.seek 0ml P%K' +740 h ' ."MFBPCR"delay 5 seconds +500 F*G9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*/ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*N% h % ."MFBPCR"\AS.seek 0.041ml F*'% +336 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%-' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%F +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%V' +2610 h ' .t\PSV.dial PSR P%R +113 h P%!% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%Y' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%8  +38 h  .t\PS.seek 0ml P%Q' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%O' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %`'k +2678 h 'k .t\PSV.dial :waste1 P%@  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %_' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*e I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *B'V +849 h ' .t\PSV.dial :air P%U  +113 h  .t\PS.seek 2.5ml P%X' +2610 h ' .t\PSV.dial :mixloop P%j +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %I' +5107 h ' .t\PSV.dial :air P%c  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %Z' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*h  +42 h  F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *k'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*9 I +43 h I F*a% h %Q ."MFBPCR"\AS.seek 0.01ml F*f' +171 h ' .t\PSV.dial :mixloop P%d +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %#' +618 h ' ."MFBPCR"\ATV.dial :waste F*t n +129 h n ."MFBPCR"\AS.seek :empty F*o'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*v  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*C  +43 h F*^% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'T  +38 h  C'y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'}'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'H' +1625 h ' ."connect"\CSV.dial CTV C'n  .t\CTV.dial CSV C'u  +38 h  +113  .t\CBV.dial :waste1 C'r @ +37 h @ .t\CS.seek 0ml C'{'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*  +21 h ."MFBPCR"delay 3 seconds +300 F*% h %_ ."MFBPCR"\AS.seek 0.434ml F*'ݏ +110 h '4 .t\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'|'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*\ % h %ݏ ."MFBPCR"\AS.seek 0.419ml F*'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*z n +108 h n F*% h %& ."MFBPCR"\AS.seek 0.092ml F* '&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'q' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +6 h (ӷ M&~& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&b/ +150 h / .t\CC.seek :open C' ( +416 h ( M&L& h &ӷ M&m% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +90 h 'η .tHand.open M&21 +150 h 1 M&3% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1200ml #Loading wcr puck from tube 4 into Collection Clamp C' & h & M&% h %\  A(1% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b (i(,9 +201 h (,9 ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ((& h &,9 M&%& h &- M&% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&$& h &w A(s& h &,9 ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &,' +89 h ' A(<& h &,9 ."WCR"\SE.seek 18.71 Pucks below Top Plate A("' +77 h ' A(& h &,9 M&?& h &w ."WCR"\Elbow.seek 4 M&0(w +6 h (w M&E& h &w A(5& h &,9 ."WCR"\Forearm.seek 4 + 300 counts M&)'7 +23 h '7 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&:& A(&'X h &w M&*% h %7 ."WCR"\Forearm.seek :retract M&'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&6(ӷ +142 h (ӷ M&7& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' ."WCR"\CC.seek :closed C';(W +3 ."MFBPCR"\ATV.dial :waste2 F*D % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *M'R +370 h (W ."WCR"Hand.open M&'1 +150 h 1 M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*/  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *N' +29 h '\  ."WCR"\SC.coast #collectSample 1200ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)=+ +6 h + S)% h % C'K& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'F  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*M  +21 h F*i% h %X ."MFBPCR"\AS.seek 0.24ml F*=' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*R n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*!  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *Y'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*- @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*4'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*['(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*@  +43 h  #"MFBPCR"\Swish #1 of 2 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *e'\  +1031 h '\  F*W% h %\  ."MFBPCR"\AS.seek 0.5ml F*U'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*O% h %(A ."MFBPCR"\AS.seek 0.6ml F*X'\  +1032 h '\  F*c% h %\  ."MFBPCR"\AS.seek 0.5ml F*I'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*h% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *_' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*j % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +3472 h 'V ."MFBPCR"\SPE.coast F*9  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0103h1200ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*a  +65 h  ."MFBPCR"\ARV.dial :carrier F*d  +10 h  F*k% h %V ."MFBPCR"\AS.seek 0.5ml F*]'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*N n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *o'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*P  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*y% h %Fj ."MFBPCR"\AS.seek 0.524ml F*T' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*}  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*n% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*r % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*x% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*z' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*q'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +112239 #"WCR"\Sampled 1200.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'~  +76 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +37 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'b' +910 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C'\\ @ +75 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C'g  +94 h  C'L% h % ."WCR"\CSV.dial between air and CTV C'l ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'm  '+  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'2  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'3  +37 h  C'% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'p' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '(' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +76 h @ +50 S)1% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'% ` +56 h ` ."WCR"\CS.seek 7.288ml C's' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'<  ."WCR"\CSV.dial CTV C'$  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1920 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'?  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'5  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'E% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ':' +816 h ' #"WCR"\Pumping wcr puck from tube 4 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'*  +94 h  C'A% h % ."WCR"\CSV.dial between air and CTV C') ` +57 h ` ."WCR"\CS.seek 7.288ml C'7' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'D  '&  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '0'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'"  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ' ' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 3 M&J% h %\  A(/% h %X A('. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (K( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (F& h & M&& h &ӷ M&M% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &i( +143 h ( M&>& h & A(R& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &!' +89 h ' A(=& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(8'K +254 h 'K M&V& h & A(Y& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 3 M&#Q@2^d^2b &' +25 h ' ."WCR"\Elbow.hold M&Q, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(4- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(@' +27 h ' M&`% h % A([& h & ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &W'B +21 h 'B M&e& h & A(O& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &U' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(c' +36 h ' M&X& h & M&h% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +90 h '\  ."WCR"\Elbow.seek CC M&j(ӷ +142 h (ӷ M&_& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ ."WCR"\Forearm.seek CC M&a' +91 h ' ."WCR"Hand.close M&d/ +150 h / ."WCR"\CC.seek :open C'k( +416 h ( A(B& h & A(]& h & M&N& h &ӷ M&t% h % ."WCR"\Forearm.seek :retract M&v'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &f( +142 h ( M&C& h & A(^& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &S' +93 h ' ."WCR"\Elbow.hold M&P, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &y1 +150 h 1 M&o% h % A(}& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 3 + 300 counts +2 M&n'B +21 h 'B M&T& h & A(r& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &H' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (x% h %B M&u% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'{& h & .tHand.open M&1 + A(6 + h 1 M&\ & h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&z& h &- M&|% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &\\' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'g& h &W .tHand.open M&L1 +150 h 1 M&l% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'm& h &W C'+% h % .t\CSV.dial CSR C'  +37 h  C'2% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'p& h &W C' % h %4 .t\CSV.dial :flush C'1  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'%% h %p .t\CS.seek 7ml C'('i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'$  '<  +38 h  +112  .t\CBV.dial :waste1 C's @ +76 h @ .t\CS.seek 0ml C'?'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'5% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C',  +38 h  .t\CBV.dial :waste2b C'*  +75 h  .t\CS.seek 0ml C'A'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C')  +37 h  C':% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'D'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '&' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  +37 h  .t\CBV.dial :waste1 C'" @ +76 h @ .t\CS.seek 0ml C'0'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'J'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +709 h ' ."connect"\CSV.dial CTV C''  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'F  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'M  +38 h  C'>% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'R'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +710 h ' ."connect"\CSV.dial CBV C'6 .t\CBV.dial CSV C'G  +38 h +75 .t\CTV.dial :air C'V  +150 h .t\CS.seek 0ml C'Y'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'['4 +507 h '4 #t\Flushing upper manifold C'e& h &W C'O% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'i% h %p .t\CS.seek 7ml C'8'i +415 h 'i ."connect"\CSV.dial CBV C' .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'_'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'9'0 +507 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'@'4 +506 h '4 C'U& h &W C'Q% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'I'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'; @ +38 h @ C'B% h %0 .t\CS.seek 5ml C']'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'N'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'C +75 h .t\CS.seek 0ml C'^'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'a& h &W C'j% h %4 .t\CSV.dial :flush C'y  +37 h  .t\CS.seek 5ml C'o'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +37 h @ C'T% h %p .t\CS.seek 7ml C'r'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'v  +38 h  +113  .t\CBV.dial :waste1 C'f @ +37 h @ .t\CS.seek 0ml C'x'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'u& h &W C'{% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'S% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'c  +37 h  .t\CBV.dial :waste2b C'H  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &k'η +91 h 'η .tHand.open M&g1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'm& h & .tHand.open M&+1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &2(- +7 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&3% h %η .t\Forearm.seek :retract M&p'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&1& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'%(W +416 h (W C'& h &W .tHand.open M&$1 +150 h 1 M&<% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &s'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'(& h &W C'% h %4 .t\CSV.dial CSR C'5  +37 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '?'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'E +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +2009 h '4 #t\Purging external Intake C'*& h &W C'% h %4 .t\CSV.dial :flush C')  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ':' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A 'D  +37 h +38 .t\CTV.dial :intake C'7  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'"'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'&  +75 h #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'0  +75 h  .t\CS.seek 5ml C'J'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'' @ +37 h @ C'F% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'M +113 h .t\CTV.dial :air .\CS.seek 0ml C'>'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'R +37 h .t\CS.seek 0ml C'G'4 +507 h '4 C'6& h &W C'V% h %4 .t\CSV.dial :flush C'=  +37 h  .t\CS.seek 3ml C'Y'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'` @ +38 h @ C'4% h %0 .t\CS.seek 5ml C'e'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'O'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'X'0 +506 h '0 .t\CSV.dial :waste C'[ +76 h .t\CS.seek 0ml C'!'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &h(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&_' +91 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C'9( +416 h ( M&U& h &ӷ M&Q% h % .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &@(- +264 h (- M&;& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &I'η +91 h 'η .tHand.open M&]1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 18tun_postDeploy3 until completion of MFBPCR +399263 !"PCR"o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0103h1200ml.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*j  +21 h ."MFBPCR"\ATV.dial :air3 F*y % +87 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *}'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*o% h %Z ."MFBPCR"\AS.seek 0.81ml F*v'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*x  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*S% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*z  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*b% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*g  +22 h F*L% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*m  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *+'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0106h1200ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*2% h %V ."MFBPCR"\AS.seek 0.5ml F*p'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *<'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*?  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F**  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*)'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*,  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*A'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*7 n +21 h n ."MFBPCR"delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.6ml F*&'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*0' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*"  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*'  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*F% h % \\ ."MFBPCR"\AS.seek 0.326ml F*J'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*M  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*/ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0106h1200ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*6 % +86 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *='Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F*4'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*e% h %> ."MFBPCR"\AS.seek 0.835ml F*O'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*i'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*8  +64 h  F*W% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*]% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *N' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*I% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*j'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*^% h %m ."MFBPCR"\AS.seek 0.82ml F*a'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*T  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *S'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0108h1200ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*\  +11 h  F*c% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*~  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +21 h ."MFBPCR"delay 2 seconds +200 F*P% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*g' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*L % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*m'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*+% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*l  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*1% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*2  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0108h1200ml.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*|  +22 h ."MFBPCR"\ATV.dial :air3 F*$ % +86 h % F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*<% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*,% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*)' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F*" % +86 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *F' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*0  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*- % +22 h % ."MFBPCR"delay 3 seconds +300 F*>% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*6% h %m ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*R  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *G'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0110h1200ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ARV.dial :carrier F*i  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*s'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*U n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Q'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *;'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*d I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*N' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*t n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*w' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*y  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*j'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*T  +108 h  ."MFBPCR"delay 7 seconds +700 F*a% h %< ."MFBPCR"\AS.seek 0.594ml F*o'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*} n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*f'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*x  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*c % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0110h1200ml.pcr F*S% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*n % +87 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*P% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*g% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *l' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*2'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*$% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *5'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0113h1200ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*,  +42 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*D% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*"  +22 h ."MFBPCR"delay 2 seconds +200 F*'% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*0 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*F' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*- % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*6% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*R  +22 h  ."MFBPCR"delay 7 seconds +700 F*V% h %< ."MFBPCR"\AS.seek 0.594ml F*G'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*` n +21 h n ."MFBPCR"delay 2 seconds +200 F*=% h %\  ."MFBPCR"\AS.seek 0.6ml F*Y'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*e  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*4% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*O  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*W'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*i  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0113h1200ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*8 @@T ."MFBPCR"\ASV.dial :SPEdelivery F*s  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*%  +150 h F*Q% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *B' +424 h ' ."MFBPCR"\ATV.dial :ASV F*h n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*_% h % ."MFBPCR"\AS.seek 0.62ml F*d'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*I n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *;'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *t'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*N % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *^' +424 h ' ."MFBPCR"\ATV.dial :air3 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*j% h % ."MFBPCR"\AS.seek 0.71ml F*}' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h % H ."MFBPCR"\AS.seek 0.735ml F*v' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*f% h % Ű ."MFBPCR"\AS.seek 0.72ml F*r' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*x n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F* % h % zG ."MFBPCR"\AS.seek 0.365ml F*{' +7162 h ' ."MFBPCR"\ATV.dial :waste F*\ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*S % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F*H' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*P% h %  ."MFBPCR"\AS.seek 0.604ml F*~'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*g % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +21 h F*m% h % ."MFBPCR"\AS.seek 0.58ml F*\\' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*1 n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*3  +22 h  ."MFBPCR"\AS.seek 0ml F*l'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*2  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *|' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*$ n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *('R +202 h 'R ."MFBPCR"\SPE.coast F*5  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*<'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*E I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*,% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b **': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*D  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *)'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*: n +64 h n F*7% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *A' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*& I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*-% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *>'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*F n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *6' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*/ I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*V  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*` n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *='Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*G n +151 h n ."MFBPCR"\AS.seek 0ml F*e'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*4  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*Y % +43 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*[% h % ."MFBPCR"\AS.seek 0.96ml F*i'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %0 ."MFBPCR"\AS.seek 0.985ml F*W' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +21 h F*Q% h % ."MFBPCR"\AS.seek 0.97ml F*U'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*h I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *_'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *I'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *;'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*t  +43 h  F*N% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *q' +9988 h ' ."MFBPCR"\ATV.dial AIV F*w I +42 h I ."MFBPCR"\AS.seek 0.02ml F*^' +9880 h ' ."MFBPCR"\ATV.dial :waste F*a n +129 h n ."MFBPCR"\AS.seek 0ml F*j'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*T% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +43 h % F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*x% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +21 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*n  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *b'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +21 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*5 n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F*( % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun_postDeploy3 assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 3, 4 => 19, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:08:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun_postDeploy3 assays" #\Mission runtime was 2:06.770658 #-> exit #m\EXIT @1543072231.53CET @1544433690.70CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:19:43CET01-Jan-70 .delayUntil "16:19:43.00" +5518299 #-> shortmfb([Tt1],[:extSrc]){}.finish !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> MFB.ready? !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted @1544435673.32CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi$; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:19:43CET01-Jan-70 .delayUntil "16:19:43.00" +5518299 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> shortmfb([Tt1], [:extSrc]){}.finish F*M% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0116h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*N  +129 h  ."MFBPCR"\ARV.dial :carrier F*O  +11 h  F*P% h %R ."MFBPCR"\AS.seek 0.499ml F*Q'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*V  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*W  +22 h ."MFBPCR"delay 2 seconds +200 F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*Z  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*]  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*` % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*b'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +107 h  ."MFBPCR"delay 7 seconds +700 F*d% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*f  +22 h  ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*m  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*n% h % \\ ."MFBPCR"\AS.seek 0.326ml F*o'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi$; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0116h.pcr F*r% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*t  +22 h ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +21 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5059 h 'V #t-> exit #m\EXIT @1544435767.72CET @1544446824.18CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 18:40:16CET01-Jan-70 .delayUntil "18:40:16.00" +6361599 #-> execute 'stdMER2017_mwj.rb' !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"DNo 'stdMER2017_mwj.rb' script found on /home/esp/esp2/mission:. #-> execute 'stdMER2017_MWJ.rb' .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0118h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*] n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*` I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0118h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*w  +22 h ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +21 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +129 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +626 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0121h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*>  +129 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +21 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*I n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*J I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0121h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0123h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*4 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*7 I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*; % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +107 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +22 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET87880.48 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0123h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +21 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +129 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +626 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0201h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +129 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*+ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +21 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0201h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2531 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +625 h ' M .t\ATV.dial :waste F*r n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3334 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0204h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*y  +130 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0204h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +21 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +31 h  ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +21 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*X  +108 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +620 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R F*b% h %R #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0206h.pcr #Building PCR Slug from extSrc with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*f  +129 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *g'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +21 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*k n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*q I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*x % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0206h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*C  +107 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*H  +129 h  F*J% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *K'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*N  +129 h F*I% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *L' ~ +625 h ' ~ .t\ATV.dial :waste F*G n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +32 h 'V .t\ATV.dial :air3 F*S % +43 h % F*R% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*P% h % .t\AS.seek 0.31ml F*Y'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % .tdelay 3 seconds +300 F*X% h %m\  .t\AS.seek 0.335ml F*Z' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*[% h % .t\AS.seek 0.32ml F*`'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*W  +43 h  .t\AS.seek 0ml F*a'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *^'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *_'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *f'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *i'V +10077 h 'V .t\ATV.dial :air2 F*d  +108 h  F*g% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *l'(@ +5053 h '(@ .t\ATV.dial :extSrc F*c  +108 h  F*j% h %(@ .t\AS.seek 0.02ml F*m' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*h n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +31 h 'V .t\ATV.dial :air3 F*k % +43 h % F*t% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *s' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*r% h % .t\AS.seek 0.31ml F*p'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % .tdelay 3 seconds +300 F*y% h %m\  .t\AS.seek 0.335ml F*u' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*w% h % .t\AS.seek 0.32ml F*{'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*v'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +107 h  F*\ % h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +108 h  F* % h %(@ .t\AS.seek 0.02ml F* ' +4851 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F* % h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.31ml F*'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*"' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*(  +43 h  .t\AS.seek 0ml F*'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +10077 h 'V .t\ATV.dial :air2 F*2  +107 h  F*'% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *%'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +107 h  F*1% h %(@ .t\AS.seek 0.02ml F*0' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*3 n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +145 h 'R #t-> exit #m\EXIT @1544446961.71CET @1544525079.31CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refih!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 09:25:53CET02-Jan-70 .delayUntil "09:25:53.00CET02-Jan-70" @CET116753.00 #-> execute 'stdMER2017_MWJ.rb' .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ C' -\ P% 2\ S)&\ P% *\ C' 2\ P% 0\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0209h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*`  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +108 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refih!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0209h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +128 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +625 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0211h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*>  +130 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*I  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*J  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*P  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +108 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0211h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0214h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*4  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*7  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*;  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +108 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +21 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0214h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +128 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +625 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0216h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +22 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0216h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2531 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +626 h ' M .t\ATV.dial :waste F*r n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3334 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0218h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*y  +130 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0218h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +64 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +21 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*X  +107 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R F*b% h %R #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0221h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*f  +129 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *g'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*k  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*q  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*u' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*x  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0221h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*C  +108 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +423 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*H  +129 h  F*J% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *K'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*N  +129 h F*I% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *L' ~ +625 h ' ~ .t\ATV.dial :waste F*G n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +32 h 'V .t\ATV.dial :air3 F*S % +43 h % F*R% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *V' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*P% h % .t\AS.seek 0.31ml F*Y'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % .tdelay 3 seconds +300 F*X% h %m\  .t\AS.seek 0.335ml F*Z' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*[% h % .t\AS.seek 0.32ml F*`'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*W  +43 h  .t\AS.seek 0ml F*a'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *]'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *^'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *_'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *f'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *i'V +10077 h 'V .t\ATV.dial :air2 F*d  +108 h  F*g% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *l'(@ +5053 h '(@ .t\ATV.dial :extSrc F*c  +108 h  F*j% h %(@ .t\AS.seek 0.02ml F*m' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*h n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +32 h 'V .t\ATV.dial :air3 F*k % +43 h % F*t% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *s' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*r% h % .t\AS.seek 0.31ml F*p'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*x % +21 h % .tdelay 3 seconds +300 F*y% h %m\  .t\AS.seek 0.335ml F*u' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +21 h F*w% h % .t\AS.seek 0.32ml F*{'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*v'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *}'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +108 h  F*\ % h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5053 h '(@ .t\ATV.dial :extSrc F*  +108 h  F* % h %(@ .t\AS.seek 0.02ml F* ' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F* % h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % .t\AS.seek 0.31ml F*'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*"' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*(  +43 h  .t\AS.seek 0ml F*'V @CET169256.52 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b */'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +10077 h 'V .t\ATV.dial :air2 F*2  +107 h  F*'% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *%'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +107 h  F*1% h %(@ .t\AS.seek 0.02ml F*0' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*3 n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +146 h 'R #t-> exit #m\EXIT @1544525168.17CET @1544525177.40CET = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi0; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 00:11:32CET03-Jan-70 .delayUntil "00:11:32.00CET03-Jan-70" @CET169892.00 #-> ESP.ready? #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready? F*D% h %&K #t-> MFB.ready! F*E% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *F% h %&K .t\ATV.dial :waste2 F*G % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *H' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*I' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*J n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *K'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*L  +12 h  .t\ASV.dial :waste1 F*M  h #t-> exit #m\EXIT @1544525243.23CET @1544681377.32CET = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 00:14:02CET03-Jan-70 .delayUntil "00:14:02.00CET03-Jan-70" @CET170042.00 #-> execute 'stdMER2017_MWJ.rb' .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0300h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*] I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*` n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +108 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +21 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0300h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +22 h ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +129 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +625 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3145 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0302h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*>  +129 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*I I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*J n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*P  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +22 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0302h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +21 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +87 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1023 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +128 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +129 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +625 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0305h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*4 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*7 n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*;  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +108 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +22 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0305h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +21 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +87 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +129 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +626 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0307h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +129 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +22 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0307h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*>  +21 h ."MFBPCR"\ATV.dial :air3 F*: % +87 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +21 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +65 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +86 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +21 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +21 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*o  +108 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +128 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2532 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +625 h ' M .t\ATV.dial :waste F*r n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3335 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0309h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*y  +129 h  ."MFBPCR"\ARV.dial :carrier F*{  +11 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0309h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*,  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +65 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +21 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*X  +108 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +620 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R F*b% h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0312h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*f  +130 h  ."MFBPCR"\ARV.dial :carrier F*e  +10 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *g'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*k I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*q n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*u' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*x  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +108 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0312h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*/ % +86 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +21 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5060 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*C  +107 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*H  +129 h  F*J% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *K'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*N  +129 h F*I% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *L' ~ +625 h ' ~ .t\ATV.dial :waste F*G n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +3294 h 'R F*M% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0314h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*S  +129 h  ."MFBPCR"\ARV.dial :carrier F*R  +11 h  F*V% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *O'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +21 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *`'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*W  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*^  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*_' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*b % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*d% h %< ."MFBPCR"\AS.seek 0.594ml F*g'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*l  +22 h  ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*m  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*s'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*r % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0314h.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*y  +21 h ."MFBPCR"\ATV.dial :air3 F*u % +87 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*v'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*1% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *0'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +128 h  F*7% h %Fj .t\AS.seek 0.512ml F*5'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*8  +129 h F*4% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *9'f +625 h 'f .t\ATV.dial :waste F*6 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *>'R +3144 h 'R F*:% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0316h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*@  +130 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*A% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *<'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*D  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*K  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*L' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*M % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +107 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*O'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*Y  +21 h  ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Z  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*a% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0316h.pcr F*]% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *d'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*h'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste F* n +87 h n .t\AS.seek 0ml F*'V +6 h 'V .t\ATV.dial :air3 F* % +43 h % F*% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*"% h % .t\AS.seek 0.31ml F*('m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*/'V +6456 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *''V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *1'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +10076 h 'V .t\ATV.dial :air2 F*7  +108 h  F*0% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *8'(@ +5053 h '(@ .t\ATV.dial :extSrc F*4  +108 h  F*5% h %(@ .t\AS.seek 0.02ml F*6' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*9 n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +145 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *@'V +32 h 'V .t\ATV.dial :air3 F*= % +43 h % F*A% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *>' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*F% h % .t\AS.seek 0.31ml F*;'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % .tdelay 3 seconds +300 F*E% h %m\  .t\AS.seek 0.335ml F*?' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*C% h % .t\AS.seek 0.32ml F*D'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*N  +43 h  .t\AS.seek 0ml F*J'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *G'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *M'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *L'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +10077 h 'V .t\ATV.dial :air2 F*R  +108 h  F*Y% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *P'(@ +5053 h '(@ .t\ATV.dial :extSrc F*O  +108 h  F*Z% h %(@ .t\AS.seek 0.02ml F*T' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*U n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *W'V +32 h 'V .t\ATV.dial :air3 F*a % +43 h % F*[% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*\\  +21 h F*]% h % .t\AS.seek 0.31ml F*`'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % .tdelay 3 seconds +300 F*_% h %m\  .t\AS.seek 0.335ml F*i' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*b  +21 h F*e% h % .t\AS.seek 0.32ml F*l'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*d  +43 h  .t\AS.seek 0ml F*g'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *c'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *o'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +10077 h 'V .t\ATV.dial :air2 F*k  +108 h  F*r% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *p'(@ +5053 h '(@ .t\ATV.dial :extSrc F*s  +108 h  F*y% h %(@ .t\AS.seek 0.02ml F*u' +4851 h ' .tdelay 1 second +100 .\ATV.dial :waste F*| n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +146 h 'R #t-> exit #m\EXIT @1544681498.35CET @1545140590.93CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refit!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 19:53:38CET03-Jan-70 .delayUntil "19:53:38.00CET03-Jan-70" @CET240818.00 #-> execute 'stdMER2017_MWJ.rb' .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0320h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*`  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refit!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0320h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +128 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +625 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0322h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*>  +130 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*I  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET256680.47 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0322h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0400h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*4  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*7  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*; % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +107 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +21 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0400h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +128 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +625 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0403h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*+ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +22 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0403h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2531 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +626 h ' M .t\ATV.dial :waste F*r n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3334 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0405h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*y  +130 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0405h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +64 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +21 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*X  +107 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R !to:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"uninitialized constant T1t: name:T1t #-> exit #m\EXIT @1545140737.63CET @1545140815.64CET = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiP!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 07:39:58CET04-Jan-70 .delayUntil "07:39:58.00CET04-Jan-70" @CET283198.00 #-> execute 'stdMER2017_MWJ.rb' .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *?' * +626 h ' * .tdelay 5 seconds +500 F*@% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *A' p +1022 h ' p .tdelay 20 seconds +2000 F*B% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *C'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*D% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *E'\ +1022 h '\  .tdelay 20 seconds +2000 F*F% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b *G' Z +626 h ' Z .tdelay 5 seconds +500 F*H% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *I' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *J' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*K  +128 h F*L% h % . .t\AS.seek 0.725ml F*M' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*N n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +7324 h 'R F*P% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0407h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*Q  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *T'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *W'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*Z  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\\'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*^% h %Fj ."MFBPCR"\AS.seek 0.524ml F*_' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*`  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*f  +107 h  ."MFBPCR"delay 7 seconds +700 F*g% h %< ."MFBPCR"\AS.seek 0.594ml F*h'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*i  +22 h  ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*k'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*l  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*r'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*t % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refiP!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0407h.pcr F*u% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*w  +21 h ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*}'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*2% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +128 h  F*6% h %Fj .t\AS.seek 0.512ml F*7'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*5  +129 h F*9% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *:'f +625 h 'f .t\ATV.dial :waste F*8 n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *<'R +3144 h 'R F*;% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0410h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*>  +130 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*@% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *?'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*E  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *G'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*I  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*K% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*J  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*M' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*P % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*S  +107 h  ."MFBPCR"delay 7 seconds +700 F*O% h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*V  +21 h  ."MFBPCR"delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.6ml F*W'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*]  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*^% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\\'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*a % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0410h.pcr F*_% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*d  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *g'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*i% h %Z ."MFBPCR"\AS.seek 0.81ml F*j'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*m'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +64 h  F*s% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*| % +86 h % F*z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *~' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\ ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.53ml F*' +2029 h ' .tdelay 10 seconds +1000 .\ATV.dial :air1 F*"  +128 h F*% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.56ml F*#,,'Td20 b *' U +626 h ' U .t\ATV.dial :waste F*( n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +3247 h 'R F*+% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0412h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*,  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *%'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*/ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *0'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*1  +22 h ."MFBPCR"delay 2 seconds +200 F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*4  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*5% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*7  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*:' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*; % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*>% h % ."MFBPCR"\AS.seek 0.536ml F*='< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*@  +107 h  ."MFBPCR"delay 7 seconds +700 F*<% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*A  +21 h  ."MFBPCR"delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*F  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.65ml F*H' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*C  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*K% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*N % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0412h.pcr F*L% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *P'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *O'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*V% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*^  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*[  +65 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *a'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*_' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*e  +32 h  ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*g% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *h' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*l  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*k'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*s% h %m ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*t  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*r' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *}'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +108 h F*% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj .t\ATV.dial :extSrc F*  +128 h  F*% h %Fj .t\AS.seek 0.512ml F*'d +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*  +129 h F*% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b * 'f +625 h 'f .t\ATV.dial :waste F*\ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +3144 h 'R F* % h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0414h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*"% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*(% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*+ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*%'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*/  +22 h  ."MFBPCR"delay 2 seconds +200 F*'% h %\  ."MFBPCR"\AS.seek 0.6ml F*0'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*3' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*4  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*5% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*7  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*8 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0414h.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*>  +22 h ."MFBPCR"\ATV.dial :air3 F*: % +86 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *<'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*=  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*? % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*D  +22 h F*C% h %n ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*H  +64 h  F*J% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *N'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*R  +32 h  ."MFBPCR"\ATV.dial :air3 F*V % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +21 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*X % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*`% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*a  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *_'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*m% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *k' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*n  +129 h  F*s% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.545ml F*#,,'T0 b *q' \ { +2531 h ' \ { .tdelay 10 seconds +1000 .\ATV.dial :air1 F*t  +129 h F*p% h % \ { .t\AS.reconfigure ASslow1 .\AS.seek 0.575ml F*#,,'Td20 b *u' M +626 h ' M .t\ATV.dial :waste F*r n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3334 h 'R F*x% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0417h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*y  +130 h  ."MFBPCR"\ARV.dial :carrier F*{  +10 h  F*|% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *z'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*"% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*( % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0417h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *+'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*,  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b **'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*1'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*0  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*5  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +64 h  F*7% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*9' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*=  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +86 h % F*<% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *?' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +21 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*D'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %> ."MFBPCR"\AS.seek 0.835ml F*K'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*J% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *L'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *U'V +5060 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *T'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*X  +107 h F*Z% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj .t\ATV.dial :extSrc F*[  +129 h  F*`% h %Fj .t\AS.seek 0.512ml F*^'d +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*a  +129 h F*]% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *_'f +625 h 'f .t\ATV.dial :waste F*\\ n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +3144 h 'R F*b% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0419h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*f  +129 h  ."MFBPCR"\ARV.dial :carrier F*e  +11 h  F*i% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *g'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*l n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *j'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*m  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *s'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*k  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*t% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*q  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*u' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*x % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.536ml F*{'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*|  +107 h  ."MFBPCR"delay 7 seconds +700 F*w% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*}'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0419h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*"  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *('n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*0'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*1% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *9'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*6' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*C  +108 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *D' +423 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*H  +129 h  F*J% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *K'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*N  +129 h F*I% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *L' ~ +625 h ' ~ .t\ATV.dial :waste F*G n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +3294 h 'R F*M% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0421h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*S  +130 h  ."MFBPCR"\ARV.dial :carrier F*R  +10 h  F*V% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *O'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *U'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Z  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*T  +22 h ."MFBPCR"delay 2 seconds +200 F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *`'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*W  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*a% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*^  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*b % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*d% h %< ."MFBPCR"\AS.seek 0.594ml F*g'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*l  +21 h  ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*m  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*h% h %\  ."MFBPCR"\AS.seek 0.65ml F*n' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*k  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*s'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*q  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*r % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0421h.pcr F*p% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*v'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*~'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\ 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' @CET342020.34 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F*1% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *0'Fj +621 h 'Fj .t\ATV.dial :extSrc F*3  +129 h  F*7% h %Fj .t\AS.seek 0.512ml F*5'd +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*8  +129 h F*4% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *9'f +625 h 'f .t\ATV.dial :waste F*6 n +22 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *>'R +3144 h 'R F*:% h %R #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0500h.pcr #Building PCR Slug from extSrc with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*@  +129 h  ."MFBPCR"\ARV.dial :carrier F*=  +11 h  F*A% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *<'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*?  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*D  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*K  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.53ml F*L' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*M % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +107 h  ."MFBPCR"delay 7 seconds +700 F*Q% h %< ."MFBPCR"\AS.seek 0.594ml F*O'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*Y  +22 h  ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*U'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Z  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*[' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*X  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*a% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*^  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*\\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0500h.pcr F*]% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *d'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +21 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*c'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*g % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*h'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +21 h F*o% h %n ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*n  +65 h  F*q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*}'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*~% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste F* n +87 h n .t\AS.seek 0ml F*'V +6 h 'V .t\ATV.dial :air3 F* % +43 h % F*% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*"% h % .t\AS.seek 0.31ml F*('m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*/'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *''V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *1'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *%'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +10077 h 'V .t\ATV.dial :air2 F*7  +107 h  F*0% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *8'(@ +5054 h '(@ .t\ATV.dial :extSrc F*4  +107 h  F*5% h %(@ .t\AS.seek 0.02ml F*6' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*9 n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *@'V +31 h 'V .t\ATV.dial :air3 F*= % +43 h % F*A% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *>' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*F% h % .t\AS.seek 0.31ml F*;'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % .tdelay 3 seconds +300 F*E% h %m\  .t\AS.seek 0.335ml F*?' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*C% h % .t\AS.seek 0.32ml F*D'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*N  +43 h  .t\AS.seek 0ml F*J'V +6456 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *K'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *G'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *M'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *L'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +10076 h 'V .t\ATV.dial :air2 F*R  +108 h  F*Y% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *P'(@ +5054 h '(@ .t\ATV.dial :extSrc F*O  +107 h  F*Z% h %(@ .t\AS.seek 0.02ml F*T' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*U n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +145 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *W'V +32 h 'V .t\ATV.dial :air3 F*a % +43 h % F*[% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *^' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*\\  +22 h F*]% h % .t\AS.seek 0.31ml F*`'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % .tdelay 3 seconds +300 F*_% h %m\  .t\AS.seek 0.335ml F*i' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*e% h % .t\AS.seek 0.32ml F*l'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*d  +43 h  .t\AS.seek 0ml F*g'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *c'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *o'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *h'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *t'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *q'V +10077 h 'V .t\ATV.dial :air2 F*k  +108 h  F*r% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *p'(@ +5053 h '(@ .t\ATV.dial :extSrc F*s  +108 h  F*y% h %(@ .t\AS.seek 0.02ml F*u' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*| n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +146 h 'R #t-> exit #m\EXIT @1545140923.34CET @1550658068.04CET = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 03:03:15CET05-Jan-70 .delayUntil "03:03:15.00CET05-Jan-70" @CET352995.00 #-> shortmfb([Ss],[:elutionWater]){}.finish !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> shortmfb([Ss],[:elutionWater]){}.finish F*M% h %R #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0503h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*N  +129 h  ."MFBPCR"\ARV.dial :carrier F*O  +11 h  F*P% h %R ."MFBPCR"\AS.seek 0.499ml F*Q'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*S n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *U'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*V  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*W  +22 h ."MFBPCR"delay 2 seconds +200 F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*Z I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*[% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*] n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*`  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*b'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*c  +107 h  ."MFBPCR"delay 7 seconds +700 F*d% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*f I +108 h I ."MFBPCR"delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*i  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.65ml F*k' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*l  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*m  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*n% h % \\ ."MFBPCR"\AS.seek 0.326ml F*o'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*q % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0503h.pcr F*r% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*t  +22 h ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *w'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*y% h %Z ."MFBPCR"\AS.seek 0.81ml F*z'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %> ."MFBPCR"\AS.seek 0.835ml F*}'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*~  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V #t-> exit #m\EXIT @1550658202.65CET @1550658320.85CET = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 05:26:33CET05-Jan-70 .delayUntil "05:26:33.00CET05-Jan-70" @CET361593.00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> MFB.ready! F*D% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *E% h %&K .t\ATV.dial :waste2 F*F % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *G' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*H' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*I n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *J'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*K  +12 h  .t\ASV.dial :waste1 F*L  h #t-> PCR.lock #-> exit #m\EXIT @1550658717.95CET @1557067407.48CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiD!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 05:29:03CET05-Jan-70 .delayUntil "05:29:03.00CET05-Jan-70" @CET361743.00 #-> shortmfb([Mm, Ss],[:elutionWater]){}.finish !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> MFB.ready? !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> MFB.ready! !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> exit #m\EXIT @1557067528.79CEST @1557067727.95CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiD!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 05:29:03CET05-Jan-70 .delayUntil "05:29:03.00CET05-Jan-70" @CET361743.00 #-> ESP.configure .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X MFB.ready! F*% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *% h %&K .t\ATV.dial :waste2 F* % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*  +11 h  .t\ASV.dial :waste1 F*  h #t-> shortmfb([Mm,Ss],[:elutionWater]){}.finish F*% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0505h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.seek 0.499ml F*'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*! n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *"'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*$  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*%  +22 h ."MFBPCR"delay 2 seconds +200 F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *''Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*(  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*)% h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*+  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*,% h % ."MFBPCR"\AS.seek 0.53ml F*-' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*.  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*1  +108 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*3'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*4 I +107 h I ."MFBPCR"delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.6ml F*6'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*7  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.65ml F*9' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*:  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*<% h % \\ ."MFBPCR"\AS.seek 0.326ml F*='{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refiD!; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0505h.pcr F*@% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*B  +21 h ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*F  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*H'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*J% h %> ."MFBPCR"\AS.seek 0.835ml F*K'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F*N'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*O  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*P  +65 h  F*Q% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Y  +32 h  ."MFBPCR"\ATV.dial :air3 F*Z % +86 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*^% h % ."MFBPCR"\AS.seek 0.81ml F*_'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*b'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*d% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*f  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *q'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0507h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ARV.dial :carrier F*s  +10 h  F*t% h %V ."MFBPCR"\AS.seek 0.5ml F*u'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*w n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *x'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*z  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*{  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *}'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*~  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*\ '\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0507h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*"'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +22 h F*%% h %n ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*(  +64 h  F*)% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*+' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *-'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*1  +32 h  ."MFBPCR"\ATV.dial :air3 F*2 % +86 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *4' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +21 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*:'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*<% h %m ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*>  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *?'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *I'V +5060 h 'V #t-> exit #m\EXIT @1557067805.40CEST @1557401112.66CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi(; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 10:12:55CET05-Jan-70 .delayUntil "10:12:55.00CET05-Jan-70" @CET378775.00 #-> execute "stgMERwithoutESPready2.rb" !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"LNo 'stgMERwithoutESPready2.rb' script found on /home/esp/esp2/mission:. #-> execute "stdERwithoutESPready2.rb" !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"KNo 'stdERwithoutESPready2.rb' script found on /home/esp/esp2/mission:. #-> execute "stdMERwithoutESPready2.rb" .\AFV.select :carrierSolution !o: BusOff:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"'Analytical Fluidic is not powered! #-> MFB.ready! !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"RMFB microcontroller never signed on while reading Analytical Syringe's status:@checkpoint: omitted #-> ESP.configure .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X MFB.ready! F*% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *% h %&K .t\ATV.dial :waste2 F* % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*  +11 h  .t\ASV.dial :waste1 F*  h #t-> execute "stdMERwithoutESPready2.rb" .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc F*  +108 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.53ml F*#,,'Td20 b *' +625 h ' .tdelay 5 seconds +500 F*% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.54ml F*#,,'T0 b * 'X +1023 h 'X .tdelay 20 seconds +2000 F*!% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *"' * +625 h ' * .tdelay 5 seconds +500 F*#% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *$' p +1022 h ' p .tdelay 20 seconds +2000 F*%% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *&'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*'% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *('\ +1023 h '\  .tdelay 20 seconds +2000 F*)% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b **' Z +625 h ' Z .tdelay 5 seconds +500 F*+% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *,' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *-' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*.  +129 h F*/% h % . .t\AS.seek 0.725ml F*0' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*1 n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *2'R +7323 h 'R F*3% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0510h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*4  +130 h  ."MFBPCR"\ARV.dial :carrier F*5  +10 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *7'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*9 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*@  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*A% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*C  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +108 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*L  +22 h  ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*N'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*T% h % \\ ."MFBPCR"\AS.seek 0.326ml F*U'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refi(; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0510h.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*e% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +32 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*|% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F* % h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.536ml F*'< +2632 h '< .tdelay 10 seconds +1000 .\ATV.dial :air1 F*  +129 h F*% h %< .t\AS.reconfigure ASslow1 .\AS.seek 0.566ml F*#,,'Td20 b *' t +625 h ' t .t\ATV.dial :waste F* n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +3282 h 'R F*% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0512h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +129 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *"'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*%  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*'' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*&  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*(' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*,'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*.  +107 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*-'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*1  +22 h  ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*6' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*7  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*8'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0512h.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +87 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +21 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +21 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +31 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +22 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*_'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +21 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*o  +108 h F*q% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *r' +423 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*s  +129 h  F*p% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.551ml F*#,,'T0 b *u' ( +3135 h ' ( .tdelay 10 seconds +1000 .\ATV.dial :air1 F*v  +129 h F*t% h % ( .t\AS.reconfigure ASslow1 .\AS.seek 0.581ml F*#,,'Td20 b *x' w +625 h ' w .t\ATV.dial :waste F*y n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3369 h 'R F*{% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0515h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*|  +130 h  ."MFBPCR"\ARV.dial :carrier F*z  +10 h  F*~% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *}'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +21 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0515h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +86 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *"'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*1  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +64 h  F*-% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*G'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*T  +108 h F*V% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj .t\ATV.dial :extSrc F*X  +128 h  F*Q% h %Fj .t\AS.seek 0.512ml F*Z'd +621 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*[  +129 h F*U% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *Y'f +625 h 'f .t\ATV.dial :waste F*^ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +3144 h 'R F*]% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0517h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*a  +130 h  ."MFBPCR"\ARV.dial :carrier F*_  +10 h  F*`% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *b'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*i  +22 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*k  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*o  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*s  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*r'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +108 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*y  +22 h  ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*~' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*}  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0517h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*\ '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*! % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*%% h % ."MFBPCR"\AS.seek 0.81ml F*$'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*9  +107 h F*:% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *<' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*=  +129 h  F*6% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *;'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*@  +129 h F*8% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *>' ~ +625 h ' ~ .t\ATV.dial :waste F*C n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +32 h 'V .t\ATV.dial :air3 F*F % +43 h % F*D% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*I% h % .t\AS.seek 0.31ml F*J'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % .tdelay 3 seconds +300 F*L% h %m\  .t\AS.seek 0.335ml F*M' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*K  +21 h F*H% h % .t\AS.seek 0.32ml F*O'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*P  +43 h  .t\AS.seek 0ml F*N'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *T'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *S'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *['V +10077 h 'V .t\ATV.dial :air2 F*U  +108 h  F*Z% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *^'(@ +5053 h '(@ .t\ATV.dial :extSrc F*Y  +108 h  F*]% h %(@ .t\AS.seek 0.02ml F*a' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*_ n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +31 h 'V .t\ATV.dial :air3 F*b % +43 h % F*e% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*h% h % .t\AS.seek 0.31ml F*i'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % .tdelay 3 seconds +300 F*g% h %m\  .t\AS.seek 0.335ml F*k' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*j% h % .t\AS.seek 0.32ml F*o'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*q  +43 h  .t\AS.seek 0ml F*m'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *x'V +10077 h 'V .t\ATV.dial :air2 F*u  +107 h  F*|% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *z'(@ +5054 h '(@ .t\ATV.dial :extSrc F*{  +107 h  F*w% h %(@ .t\AS.seek 0.02ml F*}' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F*% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.31ml F* 'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F* % h %m\  .t\AS.seek 0.335ml F* ' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*\  +43 h  .t\AS.seek 0ml F*'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10076 h 'V .t\ATV.dial :air2 F*  +108 h  F*% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +107 h  F*% h %(@ .t\AS.seek 0.02ml F*!' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +145 h 'R #t-> exit #m\EXIT @1557401315.09CEST @1557402473.90CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiX; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 20:30:20CET05-Jan-70 .delayUntil "20:30:20.00CET05-Jan-70" @CET415820.00 #-> ESP.configure .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X MFB.ready! F*% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *% h %&K .t\ATV.dial :waste2 F* % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*  +11 h  .t\ASV.dial :waste1 F*  h #t-> execute "stdMERwithoutESPready2.rb" .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc F*  +108 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.53ml F*#,,'Td20 b *' +625 h ' .tdelay 5 seconds +500 F*% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.54ml F*#,,'T0 b * 'X +1023 h 'X .tdelay 20 seconds +2000 F*!% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *"' * +625 h ' * .tdelay 5 seconds +500 F*#% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *$' p +1022 h ' p .tdelay 20 seconds +2000 F*%% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *&'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*'% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *('\ +1023 h '\  .tdelay 20 seconds +2000 F*)% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b **' Z +625 h ' Z .tdelay 5 seconds +500 F*+% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *,' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *-' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*.  +129 h F*/% h % . .t\AS.seek 0.725ml F*0' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*1 n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *2'R +7323 h 'R F*3% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0520h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*4  +130 h  ."MFBPCR"\ARV.dial :carrier F*5  +10 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *7'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*9 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*@  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*A% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*C  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +108 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*L  +22 h  ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*N'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*T% h % \\ ."MFBPCR"\AS.seek 0.326ml F*U'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refiX; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0520h.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*e% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +32 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +21 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*|% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F* % h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1022 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +129 h  F*% h %Z .t\AS.seek 0.83ml F*'K +32193 h 'K .tdelay 10 seconds +1000 .\ATV.dial :air1 F*  +129 h F*% h %K .t\AS.reconfigure ASslow1 .\AS.seek 0.86ml F*#,,'Td20 b *': +625 h ': .t\ATV.dial :waste F* n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4972 h 'R F*% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0523h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *"'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*%  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*'' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*&  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*(' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*,'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*.  +108 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*-'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*1  +21 h  ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*6' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*7  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*8'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 @CET432343.54 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0523h.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +22 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +64 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +86 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +21 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*_'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*o  +108 h F*q% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *r' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*s  +129 h  F*p% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.557ml F*#,,'T0 b *u' F +3738 h ' F .tdelay 10 seconds +1000 .\ATV.dial :air1 F*v  +128 h F*t% h % F .t\AS.reconfigure ASslow1 .\AS.seek 0.587ml F*#,,'Td20 b *x' ݢ +626 h ' ݢ .t\ATV.dial :waste F*y n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3403 h 'R F*{% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0601h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*|  +130 h  ."MFBPCR"\ARV.dial :carrier F*z  +10 h  F*~% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *}'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +21 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0601h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +86 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *"'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*1  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +64 h  F*-% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*< % +86 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +21 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*G'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*T  +108 h F*V% h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *W'Fj +621 h 'Fj .t\ATV.dial :extSrc F*X  +129 h  F*Q% h %Fj .t\AS.seek 0.512ml F*Z'd +620 h 'd .tdelay 5 seconds +500 .\ATV.dial :air1 F*[  +129 h F*U% h %d .t\AS.reconfigure ASslow1 .\AS.seek 0.542ml F*#,,'Td20 b *Y'f +625 h 'f .t\ATV.dial :waste F*^ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +3145 h 'R F*]% h %R #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0603h.pcr #Building PCR Slug from extSrc with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*a  +129 h  ."MFBPCR"\ARV.dial :carrier F*_  +11 h  F*`% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *b'$X +2547 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +2904 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*h  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*i  +22 h ."MFBPCR"delay 2 seconds +200 F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *j'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*k  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*m' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*o  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*n' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*s  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*r'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*v  +108 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*u'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*y  +22 h  ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*|  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*z% h %\  ."MFBPCR"\AS.seek 0.65ml F*~' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*}  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0603h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*\ '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*! % +87 h % F* % h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h % ."MFBPCR"\AS.seek 0.81ml F*$'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*&% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*'  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *.'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*/' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *2'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*9  +107 h F*:% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *<' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*=  +129 h  F*6% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *;'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*@  +129 h F*8% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *>' ~ +625 h ' ~ .t\ATV.dial :waste F*C n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *A'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +32 h 'V .t\ATV.dial :air3 F*F % +43 h % F*D% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*G  +22 h F*I% h % .t\AS.seek 0.31ml F*J'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*E % +21 h % .tdelay 3 seconds +300 F*L% h %m\  .t\AS.seek 0.335ml F*M' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*H% h % .t\AS.seek 0.32ml F*O'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*P  +43 h  .t\AS.seek 0ml F*N'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *T'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *S'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Q'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *['V +10077 h 'V .t\ATV.dial :air2 F*U  +108 h  F*Z% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *^'(@ +5053 h '(@ .t\ATV.dial :extSrc F*Y  +108 h  F*]% h %(@ .t\AS.seek 0.02ml F*a' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*_ n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +32 h 'V .t\ATV.dial :air3 F*b % +42 h % F*e% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *d' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*c  +21 h F*h% h % .t\AS.seek 0.31ml F*i'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % .tdelay 3 seconds +300 F*g% h %m\  .t\AS.seek 0.335ml F*k' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*j% h % .t\AS.seek 0.32ml F*o'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*q  +43 h  .t\AS.seek 0ml F*m'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *y'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *x'V +10077 h 'V .t\ATV.dial :air2 F*u  +107 h  F*|% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *z'(@ +5054 h '(@ .t\ATV.dial :extSrc F*{  +108 h  F*w% h %(@ .t\AS.seek 0.02ml F*}' +4851 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F* % +43 h % F*% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.31ml F* 'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F* % h %m\  .t\AS.seek 0.335ml F* ' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F*'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*\  +43 h  .t\AS.seek 0ml F*'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +107 h  F*% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +107 h  F*% h %(@ .t\AS.seek 0.02ml F*!' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +145 h 'R #t-> exit #m\EXIT @1557402565.32CEST @1557487698.21CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 06:53:04CET06-Jan-70 .delayUntil "06:53:04.00CET06-Jan-70" @CET453184.00 #-> ESP.configure .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X MFB.ready !o:NoMethodError :bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message,undefined method `ready' for MFB:Module: name:\ ready: args[ #-> MFB.ready! F*% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *% h %&K .t\ATV.dial :waste2 F* % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*  +11 h  .t\ASV.dial :waste1 F*  h #t-> execute "stdMERwithoutESPready2.rb" .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc F*  +108 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.53ml F*#,,'Td20 b *' +625 h ' .tdelay 5 seconds +500 F*% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.54ml F*#,,'T0 b * 'X +1023 h 'X .tdelay 20 seconds +2000 F*!% h %X .t\AS.reconfigure ASslow1 .\AS.seek 0.57ml F*#,,'Td20 b *"' * +625 h ' * .tdelay 5 seconds +500 F*#% h % * .t\AS.reconfigure ASslow2 .\AS.seek 0.58ml F*#,,'T0 b *$' p +1022 h ' p .tdelay 20 seconds +2000 F*%% h % p .t\AS.reconfigure ASslow1 .\AS.seek 0.61ml F*#,,'Td20 b *&'\ QB +625 h '\ QB .tdelay 5 seconds +500 F*'% h %\ QB .t\AS.reconfigure ASslow2 .\AS.seek 0.62ml F*#,,'T0 b *('\ +1023 h '\  .tdelay 20 seconds +2000 F*)% h %\  .t\AS.reconfigure ASslow1 .\AS.seek 0.65ml F*#,,'Td20 b **' Z +625 h ' Z .tdelay 5 seconds +500 F*+% h % Z .t\AS.reconfigure ASslow2 .\AS.seek 0.66ml F*#,,'T0 b *,' L +1022 h ' L .tdelay 20 seconds +2000 .\AS.reconfigure ASslow1 .\AS.seek 0.705ml F*#,,'Td20 b *-' . +927 h ' . .tdelay 10 seconds +1000 .\ATV.dial :air1 F*.  +129 h F*/% h % . .t\AS.seek 0.725ml F*0' j +424 h ' j .tdelay 3 seconds +300 .\ATV.dial :waste F*1 n +21 h n .t\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *2'R +7323 h 'R F*3% h %R #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0607h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*4  +130 h  ."MFBPCR"\ARV.dial :carrier F*5  +10 h  F*6% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *7'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*9 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *;'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*<  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*=  +22 h ."MFBPCR"delay 2 seconds +200 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*@ I +42 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*A% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*C n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*D% h % ."MFBPCR"\AS.seek 0.53ml F*E' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*F  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*G% h % ."MFBPCR"\AS.seek 0.536ml F*H'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +107 h  ."MFBPCR"delay 7 seconds +700 F*J% h %< ."MFBPCR"\AS.seek 0.594ml F*K'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*L  +22 h  ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*N'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*Q' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*R  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*T% h % \\ ."MFBPCR"\AS.seek 0.326ml F*U'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*V  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0607h.pcr F*X% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*[ % +86 h % F*\\% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +21 h F*_% h %Z ."MFBPCR"\AS.seek 0.81ml F*`'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*c'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*d  +22 h F*e% h %n ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*h  +65 h  F*i% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *j'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +32 h  ."MFBPCR"\ATV.dial :air3 F*r % +87 h % F*s% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*u  +22 h F*v% h % ."MFBPCR"\AS.seek 0.81ml F*w'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*|% h %m ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*~  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\ 'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*  +107 h F* % h %(@ .t\AS.reconfigure ASslow2 .\AS.seek 0.51ml F*#,,'T0 b *'Z +1023 h 'Z .tdelay 5 seconds +500 .\ATV.dial :extSrc F*  +128 h  F*% h %Z .t\AS.seek 0.542ml F*'f +3235 h 'f .tdelay 10 seconds +1000 .\ATV.dial :air1 F*  +129 h F*% h %f .t\AS.reconfigure ASslow1 .\AS.seek 0.572ml F*#,,'Td20 b *' 8 +625 h ' 8 .t\ATV.dial :waste F* n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +3316 h 'R F*% h %R #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0609h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +130 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*!  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *"'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*% I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*$% h %Fj ."MFBPCR"\AS.seek 0.524ml F*'' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*& n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*)% h % ."MFBPCR"\AS.seek 0.53ml F*(' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*+  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.536ml F*,'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*.  +108 h  ."MFBPCR"delay 7 seconds +700 F*/% h %< ."MFBPCR"\AS.seek 0.594ml F*-'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*1  +22 h  ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*4  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*6' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*2  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*7  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*8'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*:  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*< % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0609h.pcr F*=% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *>'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*;  +22 h ."MFBPCR"\ATV.dial :air3 F*@ % +86 h % F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *?'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*C  +21 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*F % +22 h % ."MFBPCR"delay 3 seconds +300 F*G% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*I  +22 h F*J% h %n ."MFBPCR"\AS.seek 0.82ml F*H'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*K% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *O'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*V  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +87 h % F*X% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Z  +21 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F*\\'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Y % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %> ."MFBPCR"\AS.seek 0.835ml F*_'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*b'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*e' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *m'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *n'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*o  +107 h F*q% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *r' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*s  +129 h  F*p% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.557ml F*#,,'T0 b *u' F +3738 h ' F .tdelay 10 seconds +1000 .\ATV.dial :air1 F*v  +129 h F*t% h % F .t\AS.reconfigure ASslow1 .\AS.seek 0.587ml F*#,,'Td20 b *x' ݢ +625 h ' ݢ .t\ATV.dial :waste F*y n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *w'R +3403 h 'R F*{% h %R #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0611h.pcr #Building PCR Slug from extSrc with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*|  +130 h  ."MFBPCR"\ARV.dial :carrier F*z  +10 h  F*~% h %R ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.499ml F*#,,'T20 b *}'$X +2548 h '$X ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2903 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :extSrc F*  +22 h  ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*! % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0611h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *#'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*% % +86 h % F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *"'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*('n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*1  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*0  +65 h  F*-% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *4'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*5' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*< % +87 h % F*=% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*@% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*> % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*G'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*B  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *R'V +5059 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *S'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*T  +107 h F*V% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *W' +424 h ' .tdelay 5 seconds +500 .\ATV.dial :extSrc F*X  +129 h  F*Q% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.538ml F*#,,'T0 b *Z'J +1827 h 'J .tdelay 10 seconds +1000 .\ATV.dial :air1 F*[  +129 h F*U% h %J .t\AS.reconfigure ASslow1 .\AS.seek 0.568ml F*#,,'Td20 b *Y' ~ +625 h ' ~ .t\ATV.dial :waste F*^ n +21 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\\'R +3294 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *]'V +32 h 'V .t\ATV.dial :air3 F*a % +43 h % F*_% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*b  +22 h F*d% h % .t\AS.seek 0.31ml F*e'm\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*c % +21 h % .tdelay 3 seconds +300 F*g% h %m\  .t\AS.seek 0.335ml F*h' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*f% h % .t\AS.seek 0.32ml F*j'P +323 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*k  +43 h  .t\AS.seek 0ml F*l'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *o'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *n'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *v'V +10077 h 'V .t\ATV.dial :air2 F*t  +108 h  F*u% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *y'(@ +5053 h '(@ .t\ATV.dial :extSrc F*x  +108 h  F*{% h %(@ .t\AS.seek 0.02ml F*|' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*z n +107 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *w'V +32 h 'V .t\ATV.dial :air3 F*} % +42 h % F*% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % .t\AS.seek 0.31ml F*'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % .tdelay 3 seconds +300 F*% h %m\  .t\AS.seek 0.335ml F*' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.32ml F* 'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*  +43 h  .t\AS.seek 0ml F*\ 'V +6457 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10076 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +10077 h 'V .t\ATV.dial :air2 F*  +107 h  F*% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *'(@ +5054 h '(@ .t\ATV.dial :extSrc F*  +108 h  F*% h %(@ .t\AS.seek 0.02ml F*' +4851 h ' .tdelay 1 second +100 .\ATV.dial :waste F* n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +146 h 'R #tdeconEluateRecoveryLine extSrc .\ATV.dial :waste .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +31 h 'V .t\ATV.dial :air3 F*! % +43 h % F* % h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h % .t\AS.seek 0.31ml F*$'m\ +6055 h 'm\  .tdelay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % .tdelay 3 seconds +300 F*&% h %m\  .t\AS.seek 0.335ml F*)' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*+% h % .t\AS.seek 0.32ml F**'P +324 h 'P .tdelay 3 seconds +300 .\ATV.dial :extSrc F*'  +43 h  .t\AS.seek 0ml F*.'V +6456 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *('V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *1'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *0'V +10077 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *-'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *,'V +10077 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *4'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :extSrc .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *5'V +10077 h 'V .t\ATV.dial :air2 F*3  +107 h  F*2% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.5ml F*#,,'T20 b *7'(@ +5054 h '(@ .t\ATV.dial :extSrc F*9  +107 h  F*:% h %(@ .t\AS.seek 0.02ml F*<' +4852 h ' .tdelay 1 second +100 .\ATV.dial :waste F*= n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *6'R +145 h 'R #t-> exit #m\EXIT @1557487778.90CEST @1557849313.73CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 14:48:21CET06-Jan-70 .delayUntil "14:48:21.00CET06-Jan-70" @CET481701.00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast C' 2\ F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9  #t-> execute "stdMERwithoutESPready2.rb" .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*D'(@ +2 h '&K !to:Slide::Error\ :bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:@axisName"Analytical Syringe: mesg")Analytical Syringe notReady at 0.5ml: @replyo:I2C::Servo::AbsMove3Reply : @channeli: @statusIS:I2C::Servo::Status: enabledF: pastRLSF: pastFLSF:pastThresholdF: lostT: homeF: positioniK&: velocityi: currenti:pwmi:erri: inPressi: outPressi:deltaPressi: voltagef12.162036327933235yo: @type2F: @error: notReady: @tagiI: @sourcei/:@destinationi%:@checkpoint: omitted #-> execute "18tun.rb" !o:RuntimeError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+Mission's startTube should be 4, not 2 #-> forgetESPstate !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::messageHundefined local variable or method `forgetESPstate' for main:Object: name:forgetESPstate #-> exit #m\EXIT @1557849535.65CEST @1557849551.75CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:59:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:107\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:59:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@17:59:45, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*/ @@T *0  +22 h  .tdelay 20 seconds +2000 .\AFV.select :carrierSolution .delay 2 seconds +200 .\AS.seek 0.5ml F*1'(@ + h '&K .t\SPE.coast F*2  h Y !to:Slide::Error\ :bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:@axisName"Analytical Syringe: mesg")Analytical Syringe notReady at 0.5ml: @replyo:I2C::Servo::AbsMove3Reply : @channeli: @statusIS:I2C::Servo::Status: enabledF: pastRLSF: pastFLSF:pastThresholdF: lostT: homeF: positioniK&: velocityi: currenti:pwmi:erri: inPressi: outPressi:deltaPressi: voltagef12.162036327933235yo: @type2F: @error: notReady: @tagi6: @sourcei/:@destinationi%:@checkpoint: omitted #-> exit #m\EXIT @1557849600.78CEST @1557849718.26CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refil ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:109\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:17, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:21, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] C'/% h %4 S)2% M&1% h % %\  M&3& h &M A(4& h &  A(5% h %X C'6& h & + P%0% %7& h % & F*8% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +7 *9% h %&K .t\ATV.dial :waste2 F*: % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *;' +2409 h ' .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*<' +5284 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :waste F*= n +107 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *>'R +5065 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  .t\ASV.dial :waste1 F*@  h #t\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*A @@T .t\ASV.dial :SPEdelivery F*B  +22 h  .tdelay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*D  +21 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +424 h ' .t\ATV.dial :ASV F*G n +129 h n .t\ASV.dial :SPErecovery F*H  +43 h  F*I% h % .t\AS.seek 0.62ml F*J'\ +2033 h '\  .t\ATV.dial :waste F*K n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3153 h 'R .t\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *M'V +29 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air3 F*O % +43 h % F*P% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' .t\ATV.dial :air3 F*R% h % .t\AS.seek 0.53ml F*S' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*U% h % .t\AS.seek 0.71ml F*V' H +3641 h ' H .tdelay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % .tdelay 3 seconds +300 F*X% h % H .t\AS.seek 0.735ml F*Y' Ű +526 h ' Ű .tdelay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*[% h % Ű .t\AS.seek 0.72ml F*\\' zG +323 h ' zG .tdelay 3 seconds +300 .\ATV.dial ASV F*] n +86 h n .t\ASV.dial :SPErecovery F*^% h % zG .t\AS.seek 0.365ml F*_' +7161 h ' .t\ATV.dial :waste F*` n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +1872 h 'R .t\ATV.dial :air3 F*b % +43 h % F*c% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *d' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*f% h % .t\AS.seek 0.579ml F*g' +11485 h '  .tdelay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % .tdelay 3 seconds +300 F*i% h %  .t\AS.seek 0.604ml F*j'\ 48 +525 h '\ 48 .tdelay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*l% h %\ 48 .t\AS.seek 0.589ml F*m' +323 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*n n +86 h n .t\ASV.dial :wasteR F*o  +22 h  .t\AS.seek 0ml F*p'V +11871 h 'V .tdelay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +20131 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air3 F*s % +108 h % F*t% h %V .t\AS.seek 0.01ml F*u' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*w% h % .t\AS.seek 0.58ml F*x' q +11485 h ' q .tdelay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % .tdelay 3 seconds +300 F*z% h % q .t\AS.seek 0.605ml F*{'\ 8 +525 h '\ 8  .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*}% h %\ 8  .t\AS.seek 0.59ml F*~' +323 h '  .tdelay 3 seconds +300 .\ATV.dial ASV F* n +86 h n .t\ASV.dial :SPEdelivery F*  +21 h  .t\AS.seek 0ml F*'V +11887 h 'V .tdelay 1:00 +6000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x .tdelay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n .t\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ .tdelay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +202 h 'R .t\SPE.coast F*  h l #tRemoving Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :ASV F* n +151 h n .t\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q .t\ATV.dial ASV F* n +65 h n F*% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*+  +43 h  F*,% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*%% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *''Q +423 h 'Q .t\ATV.dial ASV F*/ n +65 h n F*1% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *2' + +2539 h ' + .t\ASV.dial :wasteD F*3 I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +2899 h 'V .t\ATV.dial :air2 F*5  +65 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +3042 h ': .t\ATV.dial :ASV F*0 n +65 h n .t\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *7'Q +1463 h 'Q .t\ATV.dial :waste F*8 n +150 h n .t\AS.seek 0ml F*9'V +313 h 'V .t\ASV.dial :SPEdelivery F*:  +86 h  #tdeconLysateINT .\ATV.dial :air3 F* % +43 h % F*;% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*?% h % .t\AS.seek 0.96ml F*@'0 +19125 h '0 .tdelay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % .tdelay 3 seconds +300 F*>% h %0 .t\AS.seek 0.985ml F*D' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*C% h % .t\AS.seek 0.97ml F*G'c +324 h 'c  .tdelay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I .t\AIV.dial :coilToMFB C'I { +73 h { .t\AWV.select :waste C' +6 .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +198 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *K'R +10087 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R .t\ATV.dial :air2 F*P  +43 h  F*N% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +222 h ' .t\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Q' +9988 h ' .t\ATV.dial AIV F*T I +43 h I .t\AS.seek 0.02ml F*U' +9880 h ' .t\ATV.dial :waste F*S n +129 h n .t\AS.seek 0ml F*W'V +227 h 'V .t\AWV.select :air C' +6 F*X% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 F*Z  +22 h .t\ATV.dial :air3 F*[ % +43 h % F*Y% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*\\% h %Z .t\AS.seek 0.81ml F*`'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % .tdelay 3 seconds +300 F*b% h %> .t\AS.seek 0.835ml F*c'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*e% h %n .t\AS.seek 0.82ml F*f'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*d  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*i% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *g'n +2036 h 'n .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*k' +3070 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V .t\ARV.dial between carrier and air1 F*p  +32 h  .t\ATV.dial :air3 F*q % +86 h % F*s% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*v% h % .t\AS.seek 0.81ml F*w'> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % .tdelay 3 seconds +300 F*y% h %> .t\AS.seek 0.835ml F*z'm +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*|% h %m .t\AS.seek 0.82ml F*}'q +323 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*\ n +87 h n .t\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ .t\ATV.dial :waste2 F* % +108 h % .t\AS.seek 0ml F*'V +2547 h 'V #t==================> STARTED coreNEG (phase #1) <=== #\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 18:02:37CEST14-May-19\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:21, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F* % #tLoading sh1 puck from tube 4 into Collection Clamp h %V C'& h & .tHand.open M&1 +150 h 1 M&% h %\  A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +19 h (w M&& h &w A(& h &,9 .t\Forearm.seek 4 + 700 counts M&' +90 h ' A(& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A("' +56 h ' A((& h &,9 M&+& h &w .t\Elbow.seek 4 M&,(w +7 h (w M&*& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&%'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&/& A(1'X h &w M&'% h %7 .t\Forearm.seek :retract M&3'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&2(ӷ +142 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C'6(W +416 h (W .tHand.open M&81 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'& h &W ."airStroke"\CSV.dial :waste S);% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors C'9'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  C'B @ +113 h @ +38  .tdelay 3.8 seconds +380 C'@% h %( S)E% h %5J .t\SSV.dial :spit S)C +150 h .t\SS.seek 0ml S)D' +95 h ' +121 ' .t\SSV.dial CBV S)H  +151 h  ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV .\CTV.dial :air .delay 15 seconds +1500 .\SSV.dial :spit,avoiding: :exhaust S)I +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.reconfigure SSexhale C'G  .t\SS.seek 0ml S)#.0X9 h 9xd +100 C'99 h 9|d +100 C'@9 h 9d +100 C'E9 h 94d +100 C'C9 h 9Ld +100 C'<9 h 9cd +100 C'H9 h 9{d +100 C'I9 h 9d +100 C'G9 h 9d +100 C'K9 h 9d +100 C'J9 h 9d +100 C'L9 h 9d +100 C'M9 h 9 d +100 C'P9 h 9!d +100 C'D9 h 98d +100 C'=9 h 9Od +100 C'O9 h 9gd +100 C'R9 h 9~d +100 C'T9 h 9d +100 C'F9 h 9d +100 C'S9 h 9d +100 C'X9 h 9d +100 C'U9 h 9d +100 C'Q9 h 9 d +100 C'W9 h 9d +100 C'[9 h 96d +100 C'Y9 h 9Md +100 C'^9 h 9dd +100 C'Z9 h 9{d +100 C']9 h 9d +100 C'N9 h 9d +100 C'\\9 h 9d +100 C'V9 h 9d +100 C'_9 h 9d +100 C'`9 h 9 d +100 C'a9 h 9 d +100 C'c9 h 9 /d +100 C'd9 h 9 E d +100 C'b9 h 9 [d +100 C'h9 h 9 qd +100 C'i9 h 9 d +100 C'g9 h 9 d +100 C'e9 h 9  d +100 C'f9 h 9 $d +100 C'l9 h 9 (d +100 C'j9 h 9 ,d +100 C'o9 h 9\ 0d +100 C'p9 h 9\ !4d +100 C'q9 h 9\ 78d +100 C's9 h 9\ M( +23 h 'Z +392 ( A(9& h & A(@& h & M&E& h &ӷ M&C% h % .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &H( +143 h ( M&I& h & A(G& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &K' +92 h ' .t\Elbow.hold M&J, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &L1 +150 h 1 M&M% h % A(P& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &D'B +20 h 'B M&=& h & A(O& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &R' +22 h ' A(T% h %Z ."downElevator"\SE.seek :down A(F'X +100 (S% h % M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'Q @ +2 A(6 +17 h @ .t\CS.seek 10ml C'W' +6 h ' .t\CSV.dial between air and CTV C'[ ` +19 h ` .t\AIV.dial :bypass #Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&^1 +2 h 1 M&Z& h & M&]% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&`/ +150 h / M&a& h &- M&c% h %η .t\Forearm.seek :retract M&d'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +264 h (ӷ M&h& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +91 h ' .t\CC.seek :closed C'e(W +416 h (W C'f& h &W .tHand.open M&l1 +150 h 1 M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +151 h P%q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%s' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%k' +1597 h ' .t\PSV.dial PSR P%v +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%t' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%n  C'u  h +75 .t\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%x  +113 h .t\PS.seek 0ml P%|' +2610 h ' .t\PSV.dial :air P%y  +37 h  .t\PS.seek 2.5ml P%z' +2610 h ' .t\PSV.dial :mixloop P%} +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%{' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%r' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +415 h ( M& & h &ӷ M&\ % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'& h & M&% h %\  A($% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b ((& h &,9 M&& h &- M&*% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&&& h &w A()& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(/& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A('' +66 h ' A(#& h &,9 M&& h &w .t\Elbow.seek 4 M&(w +7 h (w M&5& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&"'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&1& A(4'X h &w M&,% h %7 .t\Forearm.seek :retract M&3'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&.(ӷ +142 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&!1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C':  +151 h  .t\CSV.dial CTV C'6  +19 h  .t\CS.seek 5ml C'9'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'@' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'E'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%C  +38 h  .t\PS.seek 2.5ml P%I' +2610 h ' .t\PSV.dial :mixloop P%G +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%0 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%L  +151 h  .t\PS.seek :empty P%M' +2610 h ' .t\CBV.dial :waste1 C'P @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'=  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'O @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'T  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'< ` +19 h ` .t\CSV.dial :air C'H @ +38 h @ .t\CS.seek 10ml C'S' +2647 h ' .t\CSV.dial CTV C'X  +38 h  .t\CS.seek 0ml C'K'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'Q  +150 h C'?& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&[% h %\  A(Y% h %X A(^. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Z( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (]& h & M&R& h &ӷ M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &V( +142 h ( M&F& h & A(7& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(J& h & .t\SE.seek -0.29 Pucks below Top Plate A(`'\\ +265 h '\\ M&a& h & A(c& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &U' +25 h ' .t\Elbow.hold M&_, +7 h , .t\Elbow.reconfigure TightElbow A(h- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(i' +27 h ' M&N% h % A(d& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts + M&>'B +21 h 'B M&f& h & A(l& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &j' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(p' +36 h ' M&q& h & M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  .t\Elbow.seek CC M&s(ӷ +142 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&u/ +150 h / .t\CC.seek :open C'w( +416 h ( A(x& h & A(t& h & M&y& h &ӷ M&|% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &z( +142 h ( M&~& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&{, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 3 + 300 counts +2 M&'B +21 h 'B M&b& h & A(o& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &e' +22 h ' A( % h % ."downElevator"\SE.seek :down A(\ 'X +100 (% h %S M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'$  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%(  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %&' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C')& h & .tHand.open M&/1 +2 h 1 M&& h & M&#% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&1& h &- M&,% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +265 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'"(W +415 h (W C'4& h &W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'!& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%B @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%:  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%6  +75 h  .tdelay 2 seconds +200 P%;% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%W' +1095 h ' .t\PSV.dial :air P%9  +37 h  .t\PS.seek 0ml P%@' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%C +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%r  +37 h  .tdelay 2 seconds +200 P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%0  +75 h .tdelay 2 seconds +200 P%L% h %r .t\PS.seek 0.475ml P%I' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%P  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%='N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%O  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%T  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %<' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%H +76 h .t\PTV.dial :PRV,avoiding: :puck P%E @ +75 h @ .t\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial :air P%S  +75 h  .t\PS.seek 2.5ml P%D' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%?' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%[  +75 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%^ +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%]  +76 h P%R% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %\\' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'F & +50 h & .t\AWV.select :waste C' +6 P%7% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %J'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'. { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %2' +1313 h ' .t\PSV.dial :air P%a  +38 h  .t\PS.seek 2.5ml P%c' +2609 h ' .t\PSV.dial :PML P%h +38 h .t\PS.seek 0ml P%N' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'd& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'f& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*l @@T .t\AIV.dial :bypass F*q  .t\PSV.dial PSR P%g +22 h  ."MFBPCR"delay 20 seconds +91 h P%%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%V' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +21 h I F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.3ml F*#,,'Td20 b *v': +174 .t\PSV.dial :waste1 P%k  +37 h  .t\PS.seek 0ml P%>' +1597 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%j' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%u' +1480 h ': ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +43 h  F* % h %: ."MFBPCR"\AS.seek 0.33ml F*x'ї +87 h ' .t\PSV.dial PSR P%t +113 h P%y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%|' +419 h 'ї ."MFBPCR"\ATV.dial :waste2 F*~ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1156 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%n  +38 h  .t\PS.seek 0ml P%s' +2 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +144 h ' .t\PSV.dial PSR P%b +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +112 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*}% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2455 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +635 h ' ."MFBPCR"delay 5 seconds +500 F*e9 h 9j #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*\ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Z% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +441 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%$' +2610 h ' .t\PSV.dial PSR P% +113 h P%(% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +558 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +33 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%)' +123 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2487 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %#'k +2678 h 'k .t\PSV.dial :waste1 P%5  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +106 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*{ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +954 h ' .t\PSV.dial :air P%1  +113 h  .t\PS.seek 2.5ml P%,' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1221 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *w'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*4 I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*3' +275 h ' .t\PSV.dial :mixloop P%! +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %B' +514 h ' ."MFBPCR"\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.seek :empty F*:'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*6  +86 h  F*;% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *&' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*9% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *W'_ +2775 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'C  +38 h  C'r% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'G'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'@' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'0  'L  +38 h  +113  .t\CBV.dial :waste1 C' @ +37 h @ .t\CS.seek 0ml C'P'4 +1811 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*I  +22 h ."MFBPCR"delay 3 seconds +300 F*O% h %_ ."MFBPCR"\AS.seek 0.434ml F*T'ݏ +214 h '4 .t\CSV.dial CSR C'=  +38 h  C'H% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'E'P +267 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*S% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*X'& +324 h '& ."MFBPCR"delay 3 seconds +203 h 'P .tdelay 5 seconds +97 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*['&0 +295 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'?' +1625 h ' ."connect"\CSV.dial CTV C'^  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C']'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&J/ +150 h / .t\CC.seek :open C'a( +416 h ( M&2& h &ӷ M&h% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &d(- +265 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&%1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +90 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'U& h & .tHand.open M&k1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &V(- +7 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &>& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&j/ +150 h / M&m& h &- M& % h %η .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +30 h '&0 ."MFBPCR"delay 3 seconds +61 h ' .t\CC.seek :closed C'x(W +239 ."MFBPCR"\ATV.dial :waste2 F*n % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +134 h (W C'& h &W .tHand.open M&|1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'}% h %4 .t\CSV.dial CSR C's  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +140 h 'R ."MFBPCR"\ATV.dial :air2 F*o  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +1210 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'e& h &W C'\ % h %4 .t\CSV.dial :flush C'Z  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'z'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'(& h &W C'$% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'% h %p .t\CS.seek 7ml C'M'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C')  +75 h  .t\CS.seek 0ml C'5'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'#  +38 h  C'{% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '/'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '1' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C','4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'8  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'4  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'w  +38 h  C'!% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 '6  +38 h +75 .t\CTV.dial :air C';  +151 h .t\CS.seek 0ml C':'4 +909 h '4 .tdelay 7 seconds +458 h ' ."MFBPCR"delay 5 seconds +242 .t\CS.seek 3ml C''0 +258 ."MFBPCR"\ATV.dial ASV F*9 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*&' +184 h '0 .t\CSV.dial :waste C'C +38 h .t\CS.seek 0ml C'r'4 +506 h '4 #t\Flushing upper manifold C'K& h &W C'G% h %4 .t\CSV.dial :flush C'0  +38 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'@% h %p .t\CS.seek 7ml C'I'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'O +113 h .t\CTV.dial :air .\CS.seek 0ml C'='4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'P'4 +506 h '4 C'<& h &W C'S% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.seek 3ml C'Q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'E'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'^'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C'R +75 h .t\CS.seek 0ml C'Y'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'7& h &W C'\\% h %4 .t\CSV.dial :flush C'J  +37 h  .t\CS.seek 5ml C'+'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'"% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  '.  +38 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C'q'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'F& h &W C'%% h %4 .t\CSV.dial :flush C'N  +75 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'k% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  +37 h  .t\CBV.dial :waste2b C'>  +76 h  .t\CS.seek 0ml C'g'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ .t\Forearm.seek CC M&`' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'v( +416 h ( M&u& h &ӷ M&V% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &n(- +264 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &|'η +91 h 'η .tHand.open M&1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Completed [coreNEG] begun 01:00:31 #\Extending 18tun until completion of MFBPCR +4290 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*}  +65 h  F*s% h % ."MFBPCR"\AS.seek 0.52ml F*' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*o n +64 h n ."MFBPCR"\ASV.dial :wasteR F*~  +21 h  ."MFBPCR"\AS.seek 0.3ml F*]': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *e'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*\ I +43 h I F*Z% h %N ."MFBPCR"\AS.seek 0.109ml F*p'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F* n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *z% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *(' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*$% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F**'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *M'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*# I +21 h I F*{% h %v ."MFBPCR"\AS.seek 0.059ml F*5'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*/ % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +22 h F*8% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*4  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*w'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*! I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*6'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*3 % +43 h % F*;% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*9% h % ."MFBPCR"\AS.seek 0.579ml F*C' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*K'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +21 h F*0% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*@  +43 h  ."MFBPCR"\AS.seek 0ml F*L'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*= % +108 h % F*W% h %V ."MFBPCR"\AS.seek 0.59ml F*H' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +108 h n ."MFBPCR"\ASV.dial :wasteR F*S% h %  ."MFBPCR"\AS.seek 0.09ml F*T' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*D I +86 h I F*X% h % ."MFBPCR"\AS.seek 0.12ml F*Q' +625 h ' ."MFBPCR"\ATV.dial :air3 F*E % +21 h % F*^% h % ."MFBPCR"\AS.seek 0.13ml F*R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*7% h % ."MFBPCR"\AS.seek 0.23ml F*\\' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h % ."MFBPCR"\AS.seek 0.255ml F* 'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*+% h %X ."MFBPCR"\AS.seek 0.24ml F*h' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *%'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*N @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*U I +86 h I ."MFBPCR"\AS.seek 0ml F*k'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*c'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*i  +43 h  #"MFBPCR"\Swish #1 of 2 F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*j% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*d% h %(A ."MFBPCR"\AS.seek 0.6ml F*_'\  +1031 h '\  F*y% h %\  ."MFBPCR"\AS.seek 0.5ml F*b'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*x% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *u' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*V % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3472 h 'V ."MFBPCR"\SPE.coast F*a  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0103h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*['(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +21 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*]  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*$  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F**  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 !o:#AnalyticalModule::PCRRawSample: @refil ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0103h.pcr F*{% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*/  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*!'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*@'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*<  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0106h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*Y% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*+ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*h  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *2'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*%  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*k  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*>' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*c % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*y'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +22 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*m  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0106h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*t @@T ."MFBPCR"\ASV.dial :SPEdelivery F*}  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*&  +151 h F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *~' +423 h ' ."MFBPCR"\ATV.dial :ASV F*s n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*\ '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*Z % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +423 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*e' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*p  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*z' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*$ % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F**' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F*# n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b */' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*5% h % ."MFBPCR"\AS.seek 0.579ml F*M' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*8 % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %  ."MFBPCR"\AS.seek 0.604ml F*1'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*,' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*4'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*3 % +108 h % F*!% h %V ."MFBPCR"\AS.seek 0.01ml F*;' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*6% h % ."MFBPCR"\AS.seek 0.58ml F*:' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*'% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*C  +21 h F*G% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*K n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*0'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *L'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*@  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *=' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*W n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *O'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*< n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *I'R +202 h 'R ."MFBPCR"\SPE.coast F*S  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*H'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*P n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*D  +43 h  F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *T' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*E I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*?  +43 h  F*R% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *J': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*Y  +64 h  F*7% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *\\'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*+ n +65 h n F*"% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*f I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *h'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*.  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *U': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*2  +64 h  F*k% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *i'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*q n +65 h n F*c% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *j' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*> I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *x'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*y n +151 h n ."MFBPCR"\AS.seek 0ml F*V'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*u  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*b % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*a% h % ."MFBPCR"\AS.seek 0.96ml F*['0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %0 ."MFBPCR"\AS.seek 0.985ml F*}' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*&% h % ."MFBPCR"\AS.seek 0.97ml F*o'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*A I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *]'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *~'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *Z' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*\ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*p'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*$  +22 h ."MFBPCR"\ATV.dial :air3 F*( % +43 h % F*z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*#'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*{% h %n ."MFBPCR"\AS.seek 0.82ml F*)'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*l  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b */'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*8' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*,% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *4' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +21 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*!'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*;  +22 h F*9% h %m ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*:  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *C'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *W'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *S'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*O % +108 h % ."MFBPCR"\AS.seek 0ml F*I'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 0, 2 => 0, 3 => 2, 4 => 20, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:02:39, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 1:42.550499 #-> exit #m\EXIT @1557849843.94CEST @1557849948.67CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiX; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:05:56, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:109\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:06:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:06:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] C'/% h %4 M&1% S)2% h %\  % M&3& h &M A(4& h &  A(5% h %X C'6& h & + P%0% h % P%7& h & F*8% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +7 *9% h %&K .t\ATV.dial :waste2 F*: % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *;' +2409 h ' .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*<' +5284 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :waste F*= n +107 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *>'R +5065 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  .t\ASV.dial :waste1 F*@  h #t\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*A @@T .t\ASV.dial :SPEdelivery F*B  +22 h  .tdelay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*D  +21 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +424 h ' .t\ATV.dial :ASV F*G n +129 h n .t\ASV.dial :SPErecovery F*H  +43 h  F*I% h % .t\AS.seek 0.62ml F*J'\ +2033 h '\  .t\ATV.dial :waste F*K n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3153 h 'R .t\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *M'V +29 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air3 F*O % +43 h % F*P% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' .t\ATV.dial :air3 F*R% h % .t\AS.seek 0.53ml F*S' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*U% h % .t\AS.seek 0.71ml F*V' H +3641 h ' H .tdelay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % .tdelay 3 seconds +300 F*X% h % H .t\AS.seek 0.735ml F*Y' Ű +526 h ' Ű .tdelay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*[% h % Ű .t\AS.seek 0.72ml F*\\' zG +323 h ' zG .tdelay 3 seconds +300 .\ATV.dial ASV F*] n +86 h n .t\ASV.dial :SPErecovery F*^% h % zG .t\AS.seek 0.365ml F*_' +7161 h ' .t\ATV.dial :waste F*` n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +1872 h 'R .t\ATV.dial :air3 F*b % +43 h % F*c% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *d' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*f% h % .t\AS.seek 0.579ml F*g' +11485 h '  .tdelay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % .tdelay 3 seconds +300 F*i% h %  .t\AS.seek 0.604ml F*j'\ 48 +525 h '\ 48 .tdelay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*l% h %\ 48 .t\AS.seek 0.589ml F*m' +323 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*n n +86 h n .t\ASV.dial :wasteR F*o  +22 h  .t\AS.seek 0ml F*p'V +11871 h 'V .tdelay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +20131 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air3 F*s % +108 h % F*t% h %V .t\AS.seek 0.01ml F*u' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*w% h % .t\AS.seek 0.58ml F*x' q +11485 h ' q .tdelay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % .tdelay 3 seconds +300 F*z% h % q .t\AS.seek 0.605ml F*{'\ 8 +525 h '\ 8  .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*}% h %\ 8  .t\AS.seek 0.59ml F*~' +323 h '  .tdelay 3 seconds +300 .\ATV.dial ASV F* n +86 h n .t\ASV.dial :SPEdelivery F*  +21 h  .t\AS.seek 0ml F*'V +11887 h 'V .tdelay 1:00 +6000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x .tdelay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n .t\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ .tdelay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +202 h 'R .t\SPE.coast F*  h l #tRemoving Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :ASV F* n +151 h n .t\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q .t\ATV.dial ASV F* n +65 h n F*% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*+  +43 h  F*,% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*%% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *''Q +423 h 'Q .t\ATV.dial ASV F*/ n +65 h n F*1% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *2' + +2539 h ' + .t\ASV.dial :wasteD F*3 I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +2899 h 'V .t\ATV.dial :air2 F*5  +65 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +3042 h ': .t\ATV.dial :ASV F*0 n +65 h n .t\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *7'Q +1463 h 'Q .t\ATV.dial :waste F*8 n +150 h n .t\AS.seek 0ml F*9'V +313 h 'V .t\ASV.dial :SPEdelivery F*:  +86 h  #tdeconLysateINT .\ATV.dial :air3 F* % +43 h % F*;% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*?% h % .t\AS.seek 0.96ml F*@'0 +19125 h '0 .tdelay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % .tdelay 3 seconds +300 F*>% h %0 .t\AS.seek 0.985ml F*D' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*C% h % .t\AS.seek 0.97ml F*G'c +324 h 'c  .tdelay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I .t\AIV.dial :coilToMFB C'I { +73 h { .t\AWV.select :waste C' +6 .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +198 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *K'R +10087 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R .t\ATV.dial :air2 F*P  +43 h  F*N% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +222 h ' .t\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Q' +9988 h ' .t\ATV.dial AIV F*T I +43 h I .t\AS.seek 0.02ml F*U' +9880 h ' .t\ATV.dial :waste F*S n +129 h n .t\AS.seek 0ml F*W'V +227 h 'V .t\AWV.select :air C' +6 F*X% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 F*Z  +22 h .t\ATV.dial :air3 F*[ % +43 h % F*Y% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*\\% h %Z .t\AS.seek 0.81ml F*`'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % .tdelay 3 seconds +300 F*b% h %> .t\AS.seek 0.835ml F*c'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*e% h %n .t\AS.seek 0.82ml F*f'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*d  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*i% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *g'n +2036 h 'n .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*k' +3070 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V .t\ARV.dial between carrier and air1 F*p  +32 h  .t\ATV.dial :air3 F*q % +86 h % F*s% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*v% h % .t\AS.seek 0.81ml F*w'> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % .tdelay 3 seconds +300 F*y% h %> .t\AS.seek 0.835ml F*z'm +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*|% h %m .t\AS.seek 0.82ml F*}'q +323 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*\ n +87 h n .t\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ .t\ATV.dial :waste2 F* % +108 h % .t\AS.seek 0ml F*'V +2547 h 'V #t==================> STARTED coreNEG (phase #1) <=== #\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 18:06:25CEST14-May-19\\n{1 => 0, 2 => 0, 3 => 0, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:06:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F* % h %V #tLoading sh1 puck from tube 4 into Collection Clamp C'& h & .tHand.open M&1 +150 h 1 M&% h %\  A(% h %X A(. h ., .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +356 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b (& h &,9 M&& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +19 h (w M&& h &w A(& h &,9 .t\Forearm.seek 4 + 700 counts M&' +90 h ' A(& h &,9 .t\SE.seek 20.71 Pucks below Top Plate A("' +56 h ' A((& h &,9 M&+& h &w .t\Elbow.seek 4 M&,(w +7 h (w M&*& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&%'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(1'X M&/& h &w M&'% h %7 .t\Forearm.seek :retract M&3'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&2(ӷ +142 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C'6(W +416 h (W .tHand.open M&81 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'& h &W ."airStroke"\CSV.dial :waste S);% ."airStroke"\CS.seek 0ml h % C'9'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +113 h @ +38  .tdelay 3.8 seconds +380 C'@% h %( S)E% h %5J .t\SSV.dial :spit S)C +150 h .t\SS.seek 0ml S)D' +95 h ' +121 ' .t\SSV.dial CBV S)H  +151 h  ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV .\CTV.dial :air .delay 15 seconds +1500 .\SSV.dial :spit,avoiding: :exhaust S)I +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.reconfigure SSexhale C'G  .t\SS.seek 0ml S)#.0X9 h 9td +100 C'B9 h 9xd +100 C'99 h 9|d +100 C'@9 h 9d +100 C'E9 h 94d +100 C'C9 h 9Ld +100 C'<9 h 9cd +100 C'H9 h 9{d +100 C'I9 h 9d +100 C'G9 h 9d +100 C'K9 h 9d +100 C'J9 h 9d +100 C'L9 h 9d +100 C'M9 h 9 d +100 C'P9 h 9!d +100 C'D9 h 98d +100 C'=9 h 9Od +100 C'O9 h 9gd +100 C'R9 h 9~d +100 C'T9 h 9d +100 C'F9 h 9d +100 C'S9 h 9d +100 C'X9 h 9d +100 C'U9 h 9d +100 C'Q9 h 9 d +100 C'W9 h 9d +100 C'[9 h 96d +100 C'Y9 h 9Md +100 C'^9 h 9dd +100 C'Z9 h 9{d +100 C']9 h 9d +100 C'N9 h 9d +100 C'\\9 h 9d +100 C'V9 h 9d +100 C'_9 h 9d +100 C'`9 h 9 d +100 C'a9 h 9 d +100 C'c9 h 9 /d +100 C'h9 h 9 E d +100 C'b9 h 9 [d +100 C'd9 h 9 qd +100 C'i9 h 9 d +100 C'g9 h 9 d +100 C'e9 h 9  d +100 C'f9 h 9 $d +100 C'l9 h 9 (d +100 C'j9 h 9 ,d +100 C'o9 h 9\ 0d +100 C'p9 h 9\ !4d +100 C'q9 h 9\ 78d +100 C's9 h 9\ M/ +150 h / .t\CC.seek :open C'B( +23 h 'Z +392 ( A(9& h & A(@& h & M&E& h &ӷ M&C% h % .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &H( +143 h ( M&I& h & A(G& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &K' +92 h ' .t\Elbow.hold M&J, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &L1 +150 h 1 M&M% h % A(P& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &D'B +20 h 'B M&=& h & A(O& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &R' +22 h ' A(T% h %Z ."downElevator"\SE.seek :down A(F'X +100 (S% h % M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'Q @ +2 A(6 +17 h @ .t\CS.seek 10ml C'W' +6 h ' .t\CSV.dial between air and CTV C'[ ` +19 h ` .t\AIV.dial :bypass #Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&^1 +2 h 1 M&Z& h & M&]% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&`/ +150 h / M&a& h &- M&c% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +264 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +91 h ' .t\CC.seek :closed C'e(W +416 h (W C'f& h &W .tHand.open M&l1 +150 h 1 M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +151 h P%q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%s' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%k' +1597 h ' .t\PSV.dial PSR P%v +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%t' +2610 h ' ."connect"\PRV.dial CBV P%u  .t\CBV.dial PRV C'n  h +75 .t\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%x  +113 h .t\PS.seek 0ml P%|' +2610 h ' .t\PSV.dial :air P%y  +37 h  .t\PS.seek 2.5ml P%z' +2610 h ' .t\PSV.dial :mixloop P%} +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%{' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%r' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +415 h ( M& & h &ӷ M&\ % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 4 into Collection Clamp C'& h & M&% h %\  A($% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 4 A($\ .(\ 1~b ((,9 +200 h (,9 .t\SC.reconfigure SCloose A($.(\ 1~b ((& h &,9 M&& h &- M&*% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 4 M&$&.,(\ #b &(w +166 h (w M&&& h &w A()& h &,9 .t\Forearm.reconfigure TightForearm .\Forearm.seek 4 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(/& h &,9 .t\SE.seek 19.71 Pucks below Top Plate A('' +66 h ' A(#& h &,9 M&& h &w .t\Elbow.seek 4 M&(w +7 h (w M&5& h &w A(& h &,9 .t\Forearm.seek 4 + 300 counts M&"'7 +22 h '7 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&4& A(1'X h &w M&,% h %7 .t\Forearm.seek :retract M&3'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&.(ӷ +142 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C' (W +416 h (W .tHand.open M&1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C':  +151 h  .t\CSV.dial CTV C'6  +19 h  .t\CS.seek 5ml C'9'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'@' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'E'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%C  +38 h  .t\PS.seek 2.5ml P%I' +2610 h ' .t\PSV.dial :mixloop P%G +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%!  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%0 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%L  +151 h  .t\PS.seek :empty P%M' +2610 h ' .t\CBV.dial :waste1 C'P @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'=  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'O @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'T  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'< ` +19 h ` .t\CSV.dial :air C'H @ +38 h @ .t\CS.seek 10ml C'S' +2647 h ' .t\CSV.dial CTV C'X  +38 h  .t\CS.seek 0ml C'K'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'Q  +150 h C'?& h &W #t\Storing lyfil puck in Collection Clamp to tube 3 M&[% h %\  A(Y% h %X A(^. h . .t\SC.reconfigure SCtight .\SC.seek 3 A($\ .(\ 1~b (Z( +201 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (]& h & M&R& h &ӷ M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &V( +142 h ( M&F& h & A(7& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(J& h & .t\SE.seek -0.29 Pucks below Top Plate A(`'\\ +265 h '\\ M&a& h & A(c& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 3 M&#Q@2^d^2b &U' +25 h ' .t\Elbow.hold M&_, +7 h , .t\Elbow.reconfigure TightElbow M&$&.,(\ #b A(d- h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(i' +27 h ' M&N% h % A(h& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &B'B +21 h 'B M&f& h & A(l& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &j' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(p' +36 h ' M&q& h & M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  .t\Elbow.seek CC M&s(ӷ +142 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'w( +416 h ( A(x& h & A(t& h & M&y& h &ӷ M&|% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 3 M&$&.,(\ #b &z( +142 h ( M&~& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 3 M&$ ,(#b &' +93 h ' .t\Elbow.hold M&{, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 3 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'B +21 h 'B M&b& h & A(o& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 3 + 700 counts M&#Q@2^d^\ b &e' +22 h ' A( % h % ."downElevator"\SE.seek :down A(\ 'X +100 (% h %S M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'$  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%(  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %&' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C')& h & .tHand.open M&/1 +2 h 1 M&& h & M&#% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&4& h &- M&,% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +265 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'"(W +415 h (W C'1& h &W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%> @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%:  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%6  +75 h  .tdelay 2 seconds +200 P%;% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%W' +1095 h ' .t\PSV.dial :air P%9  +37 h  .t\PS.seek 0ml P%@' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%C +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%r  +37 h  .tdelay 2 seconds +200 P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %!'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%0  +75 h .tdelay 2 seconds +200 P%L% h %r .t\PS.seek 0.475ml P%I' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%P  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%='N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%O  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%T  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %<' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%H +76 h .t\PTV.dial :PRV,avoiding: :puck P%E @ +75 h @ .t\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial :air P%S  +75 h  .t\PS.seek 2.5ml P%D' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%?' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%[  +75 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%^ +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%]  +76 h P%R% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %\\' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'F & +50 h & .t\AWV.select :waste C' +6 P%7% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %J'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'. { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %2' +1313 h ' .t\PSV.dial :air P%a  +38 h  .t\PS.seek 2.5ml P%c' +2609 h ' .t\PSV.dial :PML P%d +38 h .t\PS.seek 0ml P%N' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'h& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'f& F*l @@T h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass F*q  .t\PSV.dial PSR P%g +22 h  ."MFBPCR"delay 20 seconds +91 h P%%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%V' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +21 h I F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.3ml F*#,,'Td20 b *v': +174 .t\PSV.dial :waste1 P%k  +37 h  .t\PS.seek 0ml P%B' +1597 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%j' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%n' +1480 h ': ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +43 h  F* % h %: ."MFBPCR"\AS.seek 0.33ml F*x'ї +87 h ' .t\PSV.dial PSR P%t +113 h P%y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%|' +419 h 'ї ."MFBPCR"\ATV.dial :waste2 F*~ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1156 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%s' +2 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +144 h ' .t\PSV.dial PSR P%b +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +112 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*}% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2455 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +635 h ' ."MFBPCR"delay 5 seconds +500 F*e9 h 9j #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*\ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Z% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +441 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%$' +2610 h ' .t\PSV.dial PSR P% +113 h P%(% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +558 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +33 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%)' +123 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +2487 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %#'k +2678 h 'k .t\PSV.dial :waste1 P%5  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +106 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*{ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +954 h ' .t\PSV.dial :air P%4  +113 h  .t\PS.seek 2.5ml P%,' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1221 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *w'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*1 I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*3' +275 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %>' +514 h ' ."MFBPCR"\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.seek :empty F*:'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*6  +86 h  F*;% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *&' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*9% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *W'_ +2775 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'C  +38 h  C'r% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'G'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'@' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'0  'L  +38 h  +113  .t\CBV.dial :waste1 C'! @ +37 h @ .t\CS.seek 0ml C'P'4 +1811 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*I  +22 h ."MFBPCR"delay 3 seconds +300 F*O% h %_ .t\CS.hold ."MFBPCR"\AS.seek 0.434ml C'T+ F*='ݏ h ' +96 +* !to:IRB::Abort:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg",Collection Syringe seek to 0ml Aborted!:@checkpoint: omitted #-> exit ."MFBPCR"\SPE.coast .t\AS.stop F*H  *5 h  d !to:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"4Exiting with Collection Clamp holding FlushPuck h 'vHd #m\EXIT @1557850029.87CEST @1557850157.87CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiT ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:09:25, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:109\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:09:44, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:09:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] C'/% h %4 S)2% M&1% h % %\  M&3& h &M A(4& h &  A(5% h %X C'6& h & + P%0% %7& h % & F*8% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +7 *9% h %&K .t\ATV.dial :waste2 F*: % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *;' +2409 h ' .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*<' +5284 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :waste F*= n +107 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *>'R +5065 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  .t\ASV.dial :waste1 F*@  h #t\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*A @@T .t\ASV.dial :SPEdelivery F*B  +22 h  .tdelay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*D  +21 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +424 h ' .t\ATV.dial :ASV F*G n +129 h n .t\ASV.dial :SPErecovery F*H  +43 h  F*I% h % .t\AS.seek 0.62ml F*J'\ +2033 h '\  .t\ATV.dial :waste F*K n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3153 h 'R .t\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *M'V +29 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air3 F*O % +43 h % F*P% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' .t\ATV.dial :air3 F*R% h % .t\AS.seek 0.53ml F*S' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*U% h % .t\AS.seek 0.71ml F*V' H +3641 h ' H .tdelay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % .tdelay 3 seconds +300 F*X% h % H .t\AS.seek 0.735ml F*Y' Ű +526 h ' Ű .tdelay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*[% h % Ű .t\AS.seek 0.72ml F*\\' zG +323 h ' zG .tdelay 3 seconds +300 .\ATV.dial ASV F*] n +86 h n .t\ASV.dial :SPErecovery F*^% h % zG .t\AS.seek 0.365ml F*_' +7161 h ' .t\ATV.dial :waste F*` n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +1872 h 'R .t\ATV.dial :air3 F*b % +43 h % F*c% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *d' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*f% h % .t\AS.seek 0.579ml F*g' +11485 h '  .tdelay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % .tdelay 3 seconds +300 F*i% h %  .t\AS.seek 0.604ml F*j'\ 48 +525 h '\ 48 .tdelay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*l% h %\ 48 .t\AS.seek 0.589ml F*m' +323 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*n n +86 h n .t\ASV.dial :wasteR F*o  +22 h  .t\AS.seek 0ml F*p'V +11871 h 'V .tdelay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +20131 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air3 F*s % +108 h % F*t% h %V .t\AS.seek 0.01ml F*u' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*w% h % .t\AS.seek 0.58ml F*x' q +11485 h ' q .tdelay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % .tdelay 3 seconds +300 F*z% h % q .t\AS.seek 0.605ml F*{'\ 8 +525 h '\ 8  .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*}% h %\ 8  .t\AS.seek 0.59ml F*~' +323 h '  .tdelay 3 seconds +300 .\ATV.dial ASV F* n +86 h n .t\ASV.dial :SPEdelivery F*  +21 h  .t\AS.seek 0ml F*'V +11887 h 'V .tdelay 1:00 +6000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x .tdelay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n .t\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ .tdelay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +202 h 'R .t\SPE.coast F*  h l #tRemoving Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :ASV F* n +151 h n .t\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q .t\ATV.dial ASV F* n +65 h n F*% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*+  +43 h  F*,% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*%% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *''Q +423 h 'Q .t\ATV.dial ASV F*/ n +65 h n F*1% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *2' + +2539 h ' + .t\ASV.dial :wasteD F*3 I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +2899 h 'V .t\ATV.dial :air2 F*5  +65 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +3042 h ': .t\ATV.dial :ASV F*0 n +65 h n .t\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *7'Q +1463 h 'Q .t\ATV.dial :waste F*8 n +150 h n .t\AS.seek 0ml F*9'V +313 h 'V .t\ASV.dial :SPEdelivery F*:  +86 h  #tdeconLysateINT .\ATV.dial :air3 F* % +43 h % F*;% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*?% h % .t\AS.seek 0.96ml F*@'0 +19125 h '0 .tdelay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % .tdelay 3 seconds +300 F*>% h %0 .t\AS.seek 0.985ml F*D' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*C% h % .t\AS.seek 0.97ml F*G'c +324 h 'c  .tdelay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I .t\AIV.dial :coilToMFB C'I { +73 h { .t\AWV.select :waste C' +6 .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +198 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *K'R +10087 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R .t\ATV.dial :air2 F*P  +43 h  F*N% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +222 h ' .t\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Q' +9988 h ' .t\ATV.dial AIV F*T I +43 h I .t\AS.seek 0.02ml F*U' +9880 h ' .t\ATV.dial :waste F*S n +129 h n .t\AS.seek 0ml F*W'V +227 h 'V .t\AWV.select :air C' +6 F*X% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 F*Z  +22 h .t\ATV.dial :air3 F*[ % +43 h % F*Y% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*\\% h %Z .t\AS.seek 0.81ml F*`'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % .tdelay 3 seconds +300 F*b% h %> .t\AS.seek 0.835ml F*c'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*e% h %n .t\AS.seek 0.82ml F*f'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*d  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*i% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *g'n +2036 h 'n .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*k' +3070 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V .t\ARV.dial between carrier and air1 F*p  +32 h  .t\ATV.dial :air3 F*q % +86 h % F*s% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*v% h % .t\AS.seek 0.81ml F*w'> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % .tdelay 3 seconds +300 F*y% h %> .t\AS.seek 0.835ml F*z'm +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*|% h %m .t\AS.seek 0.82ml F*}'q +323 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*\ n +87 h n .t\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ .t\ATV.dial :waste2 F* % +108 h % .t\AS.seek 0ml F*'V +2547 h 'V #t==================> STARTED coreNEG (phase #1) <=== #\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 18:10:03CEST14-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:09:48, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F* % #tLoading sh1 puck from tube 2 into Collection Clamp h %V C'& h & .tHand.open M&1 +150 h 1 M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +20 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 700 counts M&' +90 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A("' +56 h ' A((& h &  M&+& h & .t\Elbow.seek 2 M&,( +7 h ( M&*& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&%'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(/'X M&1& h & M&'% h %8 .t\Forearm.seek :retract M&3'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&2(ӷ +142 h (ӷ M&5& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &0' +91 h ' .t\CC.seek :closed C'6(W +416 h (W .tHand.open M&81 +150 h 1 M&7% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'& h &W ."airStroke"\CSV.dial :waste .\CS.seek 0ml S);% C'9'4 h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +113 h @ +38  .tdelay 3.8 seconds +380 C'@% h %( S)E% h %5J .t\SSV.dial :spit S)C +150 h .t\SS.seek 0ml S)D' +95 h ' +121 ' .t\SSV.dial CBV S)H  +151 h  ."connect"\SSV.dial CBV,avoiding: :exhaust .t\CBV.dial SSV .\CTV.dial :air .delay 15 seconds +1500 .\SSV.dial :spit,avoiding: :exhaust S)I +151 h ."prepCSV"\CTV.dial CSV,avoiding: :intake .t\SS.reconfigure SSexhale C'G  .t\SS.seek 0ml S)#.0X9 h 9td +100 C'B9 h 9xd +100 C'99 h 9|d +100 C'@9 h 9d +100 C'E9 h 94d +100 C'C9 h 9Ld +100 C'<9 h 9cd +100 C'H9 h 9{d +100 C'I9 h 9d +100 C'G9 h 9d +100 C'K9 h 9d +100 C'J9 h 9d +100 C'L9 h 9d +100 C'M9 h 9 d +100 C'P9 h 9!d +100 C'D9 h 98d +100 C'=9 h 9Od +100 C'O9 h 9gd +100 C'R9 h 9~d +100 C'T9 h 9d +100 C'F9 h 9d +100 C'S9 h 9d +100 C'X9 h 9d +100 C'U9 h 9d +100 C'Q9 h 9 d +100 C'W9 h 9d +100 C'[9 h 96d +100 C'Y9 h 9Md +100 C'^9 h 9dd +100 C'Z9 h 9{d +100 C']9 h 9d +100 C'N9 h 9d +100 C'\\9 h 9d +100 C'V9 h 9d +100 C'_9 h 9d +100 C'`9 h 9 d +100 C'a9 h 9 d +100 C'c9 h 9 /d +100 C'h9 h 9 E d +100 C'b9 h 9 [d +100 C'd9 h 9 qd +100 C'i9 h 9 d +100 C'g9 h 9 d +100 C'e9 h 9  d +100 C'f9 h 9 $d +100 C'l9 h 9 (d +100 C'j9 h 9 ,d +100 C'o9 h 9\ 0d +100 C'p9 h 9\ !4d +100 C'q9 h 9\ 78d +100 C's9 h 9\ M/ +150 h / .t\CC.seek :open C'B( +23 h 'Z +392 ( A(9& h &( A(@& h &( M&E& h &ӷ M&C% h % .t\Forearm.seek :retract M&<'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &H( +143 h ( M&I& h & A(G& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &K'C +92 h 'C .t\Elbow.hold M&J, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &L1 +150 h 1 M&M% h %C A(P& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&D'o +20 h 'o M&=& h & A(O& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +22 h ' A(T% h %Z ."downElevator"\SE.seek :down A(F'X +100 (S% h % M&X% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +90 h '\  +81 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'Q @ +2 A(6 +17 h @ .t\CS.seek 10ml C'W' +6 h ' .t\CSV.dial between air and CTV C'[ ` +19 h ` .t\AIV.dial :bypass #Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Y& h & .tHand.open M&^1 +2 h 1 M&Z& h & M&]% h %\  .t\Elbow.seek FlushPuck::Garage M&N(- +166 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &V& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&`/ +150 h / M&a& h &- M&c% h %η .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +264 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &g' +91 h ' .t\CC.seek :closed C'e(W +416 h (W C'f& h &W .tHand.open M&l1 +150 h 1 M&j% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%p +151 h P%q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%s' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%k' +1597 h ' .t\PSV.dial PSR P%v +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%t' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%u  C'n  h +75 .t\PSV.dial :mixloop P%w +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%x  +113 h .t\PS.seek 0ml P%|' +2610 h ' .t\PSV.dial :air P%y  +37 h  .t\PS.seek 2.5ml P%z' +2610 h ' .t\PSV.dial :mixloop P%} +38 h .t\PS.seek 0ml P%' +2609 h ' .t\PSV.dial :air P%~  +38 h  .t\PS.seek 2.5ml P%{' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.seek 0ml P%r' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +415 h ( M& & h &ӷ M&\ % h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'& h & M&% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b ((& h &  M&& h &- M&*% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&&& h & A()& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &+' +89 h ' A(1& h &  .t\SE.seek 19.71 Pucks below Top Plate A('' +66 h ' A(#& h &  M&& h & .t\Elbow.seek 2 M&( +7 h ( M&5& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&"'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&4& A(/'X h & M&,% h %8 .t\Forearm.seek :retract M&3'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&.(ӷ +142 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &;' +91 h ' .t\CC.seek :closed C'$(W +416 h (W .tHand.open M& 1 +150 h 1 M&>% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C':  +151 h  .t\CSV.dial CTV C'6  +19 h  .t\CS.seek 5ml C'9'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'@' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'E'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%C  +38 h  .t\PS.seek 2.5ml P%I' +2610 h ' .t\PSV.dial :mixloop P%G +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%!  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%0 +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%L  +151 h  .t\PS.seek :empty P%M' +2610 h ' .t\CBV.dial :waste1 C'P @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'=  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'O @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'T  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'< ` +19 h ` .t\CSV.dial :air C'H @ +38 h @ .t\CS.seek 10ml C'S' +2647 h ' .t\CSV.dial CTV C'X  +38 h  .t\CS.seek 0ml C'K'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'Q  +150 h C'?& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&[% h %\  A(Y% h %X A(^. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (Z(( +201 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (]& h &( M&R& h &ӷ M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &V( +142 h ( M&F& h & A(7& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &%' +89 h ' A(J& h &( .t\SE.seek -0.29 Pucks below Top Plate A(`'\\ +265 h '\\ M&a& h & A(c& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &U'C +25 h 'C .t\Elbow.hold M&_, +7 h , .t\Elbow.reconfigure TightElbow A(d- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(i' +27 h ' M&N% h %C A(h& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &B'o +21 h 'o M&f& h & A(l& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &j' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(p' +36 h ' M&q& h & M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &m'\ +90 h '\  .t\Elbow.seek CC M&s(ӷ +142 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'w( +416 h ( A(x& h &( A(t& h &( M&y& h &ӷ M&|% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &z( +142 h ( M&~& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +93 h 'C .t\Elbow.hold M&{, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'o +21 h 'o M&b& h & A(o& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &e' +22 h ' A( % h % ."downElevator"\SE.seek :down A(\ 'X +100 (% h %S M& % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%(  +19 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %*' +250 h ' .t\PRV.dial :waste2 P% @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %&' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C')& h & .tHand.open M&11 +2 h 1 M&& h & M&#% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&5& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&4& h &- M&,% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &+(ӷ +265 h (ӷ M&8& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &'' +91 h ' .t\CC.seek :closed C'"(W +415 h (W C'/& h &W .tHand.open M&1 +150 h 1 M&3% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C' & h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%> @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%:  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%6  +75 h  .tdelay 2 seconds +200 P%;% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%W' +1095 h ' .t\PSV.dial :air P%9  +37 h  .t\PS.seek 0ml P%@' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%C +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%r  +37 h  .tdelay 2 seconds +200 P%G% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %!'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%0  +75 h .tdelay 2 seconds +200 P%L% h %r .t\PS.seek 0.475ml P%I' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%P  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%='N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%O  +37 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%T  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %<' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%H +76 h .t\PTV.dial :PRV,avoiding: :puck P%E @ +75 h @ .t\PS.seek 0ml P%X' +2610 h ' .t\PSV.dial :air P%S  +75 h  .t\PS.seek 2.5ml P%D' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%Q +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%?' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%[  +75 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%^ +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%]  +76 h P%R% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %\\' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'F & +50 h & .t\AWV.select :waste C' +6 P%7% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %J'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'. { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %2' +1313 h ' .t\PSV.dial :air P%a  +38 h  .t\PS.seek 2.5ml P%c' +2609 h ' .t\PSV.dial :PML P%d +38 h .t\PS.seek 0ml P%N' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'h& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'f& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*l @@T .t\AIV.dial :bypass F*q  .t\PSV.dial PSR P%g +22 h  ."MFBPCR"delay 20 seconds +91 h P%%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%V' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*` I +21 h I F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.3ml F*#,,'Td20 b *v': +174 .t\PSV.dial :waste1 P%k  +37 h  .t\PS.seek 0ml P%B' +1597 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%j' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%n' +1480 h ': ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +43 h  F*$% h %: ."MFBPCR"\AS.seek 0.33ml F*x'ї +87 h ' .t\PSV.dial PSR P%t +113 h P%y% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%|' +419 h 'ї ."MFBPCR"\ATV.dial :waste2 F*~ % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1156 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%s' +2 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *' +1430 h ' ."MFBPCR"delay 3 seconds +144 h ' .t\PSV.dial PSR P%b +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +112 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*}% h % ."MFBPCR"\AS.seek 0.469ml F*z' +2455 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2609 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%p' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%' +635 h ' ."MFBPCR"delay 5 seconds +500 F*e9 h 9j #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*\ n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*Z% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +441 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P% +113 h P%(% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +558 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +33 h ' .t\PSV.dial PSR P%* +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%)' +123 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2487 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %#'k +2678 h 'k .t\PSV.dial :waste1 P%5  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +106 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*{ I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +954 h ' .t\PSV.dial :air P%4  +113 h  .t\PS.seek 2.5ml P%,' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%8  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1221 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *w'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*/ I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*3' +275 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %>' +514 h ' ."MFBPCR"\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.seek :empty F*:'R +237 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*6  +86 h  F*;% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *&' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*9% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *W'_ +2775 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'C  +38 h  C'r% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'G'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'@' +1625 h ' ."connect"\CSV.dial CTV C'0  .t\CTV.dial CSV C'L  +38 h  +113  .t\CBV.dial :waste1 C'! @ +37 h @ .t\CS.seek 0ml C'P'4 +1811 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*I  +22 h ."MFBPCR"delay 3 seconds +300 F*O% h %_ ."MFBPCR"\AS.seek 0.434ml F*T'ݏ +214 h '4 .t\CSV.dial CSR C'=  +38 h  C'H% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'E'P +267 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*S% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*X'& +324 h '& ."MFBPCR"delay 3 seconds +203 h 'P .tdelay 5 seconds +97 ."MFBPCR"\ATV.dial ASV F*Q n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*['&0 +295 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'?' +1625 h ' ."connect"\CSV.dial CTV C'^  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C']'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&.' +91 h ' .tHand.close M&J/ +150 h / .t\CC.seek :open C'a( +416 h ( M&2& h &ӷ M&d% h % .t\Forearm.seek :retract M&c'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &h(- +265 h (- M&f& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&%1 +150 h 1 M&N% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &`'\ +90 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'U& h & .tHand.open M&k1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &V(- +7 h (- M&i& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.seek :garage M&_'η +91 h 'η .tHand.close M&j/ +150 h / M&m& h &- M&$% h %η .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +264 h (ӷ M&v& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +30 h '&0 ."MFBPCR"delay 3 seconds +61 h ' .t\CC.seek :closed C'x(W +239 ."MFBPCR"\ATV.dial :waste2 F*u % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +134 h (W C'& h &W .tHand.open M&|1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'}% h %4 .t\CSV.dial CSR C's  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +140 h 'R ."MFBPCR"\ATV.dial :air2 F*o  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +1210 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ' '[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'p'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'e& h &W C'\ % h %4 .t\CSV.dial :flush C'Z  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'z'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'(& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'% h %p .t\CS.seek 7ml C'M'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C')  +75 h  .t\CS.seek 0ml C'5'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'#  +38 h  C'{% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '1'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '4' +710 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C' @ +75 h @ .t\CS.seek 0ml C','4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'8  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'/  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'w  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 '6  +38 h +75 .t\CTV.dial :air C';  +151 h .t\CS.seek 0ml C':'4 +909 h '4 .tdelay 7 seconds +458 h ' ."MFBPCR"delay 5 seconds +242 .t\CS.seek 3ml C''0 +258 ."MFBPCR"\ATV.dial ASV F*9 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*&' +184 h '0 .t\CSV.dial :waste C'C +38 h .t\CS.seek 0ml C'r'4 +506 h '4 #t\Flushing upper manifold C'K& h &W C'G% h %4 .t\CSV.dial :flush C'0  +38 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'@% h %p .t\CS.seek 7ml C'I'i +416 h 'i ."connect"\CSV.dial CBV C'O .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'='4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +506 h '0 .t\CSV.dial :waste C'W +38 h .t\CS.seek 0ml C'P'4 +506 h '4 C'<& h &W C'S% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.seek 3ml C'Q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'D @ +38 h @ C'X% h %0 .t\CS.seek 5ml C'E'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'^'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'?'0 +507 h '0 .t\CSV.dial :waste C'R +75 h .t\CS.seek 0ml C'Y'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'7& h &W C'\\% h %4 .t\CSV.dial :flush C'J  +37 h  .t\CS.seek 5ml C'+'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'"% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CTV C'd  .t\CTV.dial CSV C'.  +38 h  +113  .t\CBV.dial :waste1 C'f @ +38 h @ .t\CS.seek 0ml C'q'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'F& h &W C'%% h %4 .t\CSV.dial :flush C'N  +75 h  .t\CS.seek 5ml C'c'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'U @ +38 h @ C'k% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  +37 h  .t\CBV.dial :waste2b C'B  +76 h  .t\CS.seek 0ml C'g'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&j& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &m(ӷ +6 h (ӷ M&$& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &ӷ .t\Forearm.seek CC M&`' +91 h ' .tHand.close M&_/ +150 h / .t\CC.seek :open C'v( +416 h ( M&n& h &ӷ M&V% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &u(- +264 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &|'η +91 h 'η .tHand.open M&1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Completed [coreNEG] begun 01:00:31 #\Extending 18tun until completion of MFBPCR +4290 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*}  +65 h  F*s% h % ."MFBPCR"\AS.seek 0.52ml F*' +10077 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F*o n +64 h n ."MFBPCR"\ASV.dial :wasteR F*~  +21 h  ."MFBPCR"\AS.seek 0.3ml F*]': +4446 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +87 h F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *e'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*\ I +43 h I F*Z% h %N ."MFBPCR"\AS.seek 0.109ml F*p'{ +1228 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F* n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +7 *z% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +6 *% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *(' +5754 h ' ."MFBPCR"delay 5 seconds +500 F*% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*% h % ."MFBPCR"\AS.seek 0.279ml F**'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1634 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*)% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *M'v +626 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*# I +21 h I F*{% h %v ."MFBPCR"\AS.seek 0.059ml F*5'H +625 h 'H ."MFBPCR"\ATV.dial :air3 F*1 % +21 h % F*% h %H ."MFBPCR"\AS.seek 0.069ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*8% h % ."MFBPCR"\AS.seek 0.769ml F*' q +14099 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*w'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +107 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +4416 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F*6'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*3 % +43 h % F*;% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *'' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*9% h % ."MFBPCR"\AS.seek 0.579ml F*C' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*K'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +21 h F*0% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*r' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*! n +86 h n ."MFBPCR"\ASV.dial :wasteR F*@  +43 h  ."MFBPCR"\AS.seek 0ml F*L'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*= % +108 h % F*W% h %V ."MFBPCR"\AS.seek 0.59ml F*H' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +108 h n ."MFBPCR"\ASV.dial :wasteR F*S% h %  ."MFBPCR"\AS.seek 0.09ml F*T' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*P  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*D I +86 h I F*X% h % ."MFBPCR"\AS.seek 0.12ml F*Q' +625 h ' ."MFBPCR"\ATV.dial :air3 F*E % +21 h % F*^% h % ."MFBPCR"\AS.seek 0.13ml F*R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*?  +22 h F*7% h % ."MFBPCR"\AS.seek 0.23ml F*\\' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*J % +22 h % ."MFBPCR"delay 3 seconds +300 F*Y% h % ."MFBPCR"\AS.seek 0.255ml F* 'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*+% h %X ."MFBPCR"\AS.seek 0.24ml F*d' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *2'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *%'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*N @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*U I +86 h I ."MFBPCR"\AS.seek 0ml F*k'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*c'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*i  +43 h  #"MFBPCR"\Swish #1 of 2 F*B% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +1032 h '\  F*j% h %\  ."MFBPCR"\AS.seek 0.5ml F*$'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*h% h %(A ."MFBPCR"\AS.seek 0.6ml F*_'\  +1031 h '\  F*y% h %\  ."MFBPCR"\AS.seek 0.5ml F*b'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*x% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *n' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*V % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +3472 h 'V ."MFBPCR"\SPE.coast F*a  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0103h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*['(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*| n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *s'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*o  +21 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*] I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*\  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Z% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*p n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*(% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F**  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*)'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*# % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 !o:#AnalyticalModule::PCRRawSample: @refiT ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0103h.pcr F*{% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*1  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *4'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*/'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*3% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *;'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *0' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*K  +22 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*@'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +21 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*I'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*<  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *7'V +5059 h 'V #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0106h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*R  +43 h  ."MFBPCR"\ARV.dial :carrier F*J  +11 h  F*Y% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*+ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *.'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*d  +22 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *2'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*% I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*q' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*k n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*B' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*c  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*h  +108 h  ."MFBPCR"delay 7 seconds +700 F*$% h %< ."MFBPCR"\AS.seek 0.594ml F*y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*_ n +21 h n ."MFBPCR"delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*n% h %\  ."MFBPCR"\AS.seek 0.65ml F*a' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*m  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*`'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*| % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0106h.pcr F*t% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *}'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*A  +22 h ."MFBPCR"\ATV.dial :air3 F*& % +86 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*s  +22 h F*]% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*\ % h %n ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F**% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*#'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*l % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F*)'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*M  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*;% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *9'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *''(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*C  +22 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*K  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*0' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*r  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*@' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*= % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*W% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*<  +107 h  ."MFBPCR"delay 7 seconds +700 F*I% h %< ."MFBPCR"\AS.seek 0.594ml F*S'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*H n +21 h n ."MFBPCR"delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.6ml F*D'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*T% h %\  ."MFBPCR"\AS.seek 0.65ml F*E' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*?% h % \\ ."MFBPCR"\AS.seek 0.326ml F*R'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*J  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*Y % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*+  +22 h ."MFBPCR"\ATV.dial :air3 F*" % +87 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *f'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +21 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*.'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*2'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +21 h F*i% h %n ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*c  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*j  +65 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *h'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*n  +32 h  ."MFBPCR"\ATV.dial :air3 F*b % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*a% h % ."MFBPCR"\AS.seek 0.81ml F*['> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*| % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*t'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*`  +22 h F*A% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *}'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0110h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*Z% h %V ."MFBPCR"\AS.seek 0.5ml F*e'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *p' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *z'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*(  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*l% h % ."MFBPCR"\AS.seek 0.53ml F*{' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*#  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*1% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*)  +108 h  ."MFBPCR"delay 7 seconds +700 F*M% h %< ."MFBPCR"\AS.seek 0.594ml F*5'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*8 n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*,' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*/% h % \\ ."MFBPCR"\AS.seek 0.326ml F*w'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*3  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0110h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*; @@T ."MFBPCR"\ASV.dial :SPEdelivery F*  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*:  +151 h F*6% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *9' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*C  +43 h  F*G% h % ."MFBPCR"\AS.seek 0.62ml F*''\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*K n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*r'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*L % +43 h % F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *=' +424 h ' ."MFBPCR"\ATV.dial :air3 F*W% h % ."MFBPCR"\AS.seek 0.53ml F*@' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*I% h % ."MFBPCR"\AS.seek 0.71ml F*O' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +21 h % ."MFBPCR"delay 3 seconds +300 F*P% h % H ."MFBPCR"\AS.seek 0.735ml F*S' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +21 h F*T% h % Ű ."MFBPCR"\AS.seek 0.72ml F*D' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*^ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*Q% h % zG ."MFBPCR"\AS.seek 0.365ml F*?' +7161 h ' ."MFBPCR"\ATV.dial :waste F*E n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*Y % +43 h % F*7% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +22 h F*"% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*\\ % +21 h % ."MFBPCR"delay 3 seconds +300 F*d% h %  ."MFBPCR"\AS.seek 0.604ml F*F'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +21 h F*%% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*U' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*. n +86 h n ."MFBPCR"\ASV.dial :wasteR F*k  +22 h  ."MFBPCR"\AS.seek 0ml F*i'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *c'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*j % +108 h % F*B% h %V ."MFBPCR"\AS.seek 0.01ml F*q' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*h  +22 h F*$% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*_ % +21 h % ."MFBPCR"delay 3 seconds +300 F*x% h % q ."MFBPCR"\AS.seek 0.605ml F*y'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +21 h F*b% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*V n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*a'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *|'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*N  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *[' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*` n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *A'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*t n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *o'R +202 h 'R ."MFBPCR"\SPE.coast F*&  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*s n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*]  +43 h  F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*\  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*Z% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*e n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *p' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*z I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F**% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*l n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *#' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*1 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *{'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*)  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *8'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.seek 0ml F*5'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F* % +43 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*/% h % ."MFBPCR"\AS.seek 0.96ml F*,'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +22 h F*:% h % ."MFBPCR"\AS.seek 0.97ml F*6'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *C'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *9'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *''R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*!  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *0' +9988 h ' ."MFBPCR"\ATV.dial AIV F*r I +43 h I ."MFBPCR"\AS.seek 0.02ml F*W' +9880 h ' ."MFBPCR"\ATV.dial :waste F*= n +129 h n ."MFBPCR"\AS.seek 0ml F*<'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*I% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*H  +21 h ."MFBPCR"\ATV.dial :air3 F*P % +43 h % F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *X'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +21 h F*S% h %Z ."MFBPCR"\AS.seek 0.81ml F*^'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Q % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*E'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*?  +22 h F*Y% h %n ."MFBPCR"\AS.seek 0.82ml F*7'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*J  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*+  +65 h  F*"% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *R'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*.  +32 h  ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*U% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *i' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +22 h F*j% h % ."MFBPCR"\AS.seek 0.81ml F*B'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*$'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *y'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *a'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*t n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *&'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*A % +108 h % ."MFBPCR"\AS.seek 0ml F*o'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 2, 2 => 20, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@18:10:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 1:55.883352 #-> exit #m\EXIT @1557850363.12CEST @1557931819.85CEST = =tquick =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =mquickScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "190515totalsampletest.rb" .GoodNews.suppressMail "Starting 190515totalsampletest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:50:27, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "190515totalsampletest begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/190515totalsampletest.rb:112\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:50:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:50:50, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 190515totalsampletest phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== C'/% h %4 M&2% S)1% h %\  % M&3& h &M A(4& h &  A(5% h %X C'6& h & + P%0% %7& h % & F*8% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +7 *9% h %&K .t\ATV.dial :waste2 F*: % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *;' +2409 h ' .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*<' +5284 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :waste F*= n +107 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *>'R +5065 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  .t\ASV.dial :waste1 F*@  h #t\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*A @@T .t\ASV.dial :SPEdelivery F*B  +22 h  .tdelay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air1 F*D  +21 h F*E% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *F' +424 h ' .t\ATV.dial :ASV F*G n +129 h n .t\ASV.dial :SPErecovery F*H  +43 h  F*I% h % .t\AS.seek 0.62ml F*J'\ +2033 h '\  .t\ATV.dial :waste F*K n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3153 h 'R .t\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *M'V +29 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*N'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air3 F*O % +43 h % F*P% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Q' +424 h ' .t\ATV.dial :air3 F*R% h % .t\AS.seek 0.53ml F*S' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*U% h % .t\AS.seek 0.71ml F*V' H +3641 h ' H .tdelay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % .tdelay 3 seconds +300 F*X% h % H .t\AS.seek 0.735ml F*Y' Ű +526 h ' Ű .tdelay 3 seconds +300 .\ATV.dial :bleach F*Z  +22 h F*[% h % Ű .t\AS.seek 0.72ml F*\\' zG +323 h ' zG .tdelay 3 seconds +300 .\ATV.dial ASV F*] n +86 h n .t\ASV.dial :SPErecovery F*^% h % zG .t\AS.seek 0.365ml F*_' +7161 h ' .t\ATV.dial :waste F*` n +151 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *a'R +1872 h 'R .t\ATV.dial :air3 F*b % +43 h % F*c% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *d' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*f% h % .t\AS.seek 0.579ml F*g' +11485 h '  .tdelay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % .tdelay 3 seconds +300 F*i% h %  .t\AS.seek 0.604ml F*j'\ 48 +525 h '\ 48 .tdelay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*l% h %\ 48 .t\AS.seek 0.589ml F*m' +323 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*n n +86 h n .t\ASV.dial :wasteR F*o  +22 h  .t\AS.seek 0ml F*p'V +11871 h 'V .tdelay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *r'V +20131 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air3 F*s % +108 h % F*t% h %V .t\AS.seek 0.01ml F*u' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*w% h % .t\AS.seek 0.58ml F*x' q +11485 h ' q .tdelay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % .tdelay 3 seconds +300 F*z% h % q .t\AS.seek 0.605ml F*{'\ 8 +525 h '\ 8  .tdelay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*}% h %\ 8  .t\AS.seek 0.59ml F*~' +323 h '  .tdelay 3 seconds +300 .\ATV.dial ASV F* n +86 h n .t\ASV.dial :SPEdelivery F*  +21 h  .t\AS.seek 0ml F*'V +11887 h 'V .tdelay 1:00 +6000 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air2 F*  +64 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x .tdelay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n .t\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15507 h '^ .tdelay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *\ 'R +202 h 'R .t\SPE.coast F*  h l #tRemoving Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :ASV F* n +151 h n .t\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *' +4647 h '  .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +423 h 'Q .t\ATV.dial ASV F* n +65 h n F*% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *"' + +2539 h ' + .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *('V +2899 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*+  +43 h  F*,% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': .t\ATV.dial :air2 F*  +65 h  F*%% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *''Q +423 h 'Q .t\ATV.dial ASV F*/ n +65 h n F*2% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *1' + +2539 h ' + .t\ASV.dial :wasteD F*3 I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *4'V +2899 h 'V .t\ATV.dial :air2 F*5  +65 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *6': +3042 h ': .t\ATV.dial :ASV F*0 n +65 h n .t\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *7'Q +1463 h 'Q .t\ATV.dial :waste F*8 n +150 h n .t\AS.seek 0ml F*9'V +313 h 'V .t\ASV.dial :SPEdelivery F*:  +86 h  #tdeconLysateINT .\ATV.dial :air3 F* % +43 h % F*;% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *=' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*?% h % .t\AS.seek 0.96ml F*@'0 +19125 h '0 .tdelay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % .tdelay 3 seconds +300 F*>% h %0 .t\AS.seek 0.985ml F*D' +525 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*C% h % .t\AS.seek 0.97ml F*G'c +324 h 'c  .tdelay 3 seconds +300 .\ATV.dial AIV F*H I +107 h I .t\AIV.dial :coilToMFB C'I { +73 h { .t\AWV.select :waste C' +6 .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +198 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *K'R +10087 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *O'R +10088 h 'R .t\ATV.dial :air2 F*P  +43 h  F*N% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *R' +222 h ' .t\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *Q' +9988 h ' .t\ATV.dial AIV F*T I +43 h I .t\AS.seek 0.02ml F*U' +9880 h ' .t\ATV.dial :waste F*S n +129 h n .t\AS.seek 0ml F*W'V +227 h 'V .t\AWV.select :air C' +6 F*X% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ASV.dial :waste1 F*Z  +22 h .t\ATV.dial :air3 F*[ % +43 h % F*Y% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *]'Z +222 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*\\% h %Z .t\AS.seek 0.81ml F*`'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*_ % +22 h % .tdelay 3 seconds +300 F*b% h %> .t\AS.seek 0.835ml F*c'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*a  +22 h F*e% h %n .t\AS.seek 0.82ml F*f'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*d  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*h  +64 h  F*i% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *g'n +2036 h 'n .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*k' +3070 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V .t\ARV.dial between carrier and air1 F*p  +32 h  .t\ATV.dial :air3 F*q % +86 h % F*s% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*v% h % .t\AS.seek 0.81ml F*w'> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % .tdelay 3 seconds +300 F*y% h %> .t\AS.seek 0.835ml F*z'm +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*x  +22 h F*|% h %m .t\AS.seek 0.82ml F*}'q +323 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*{  +22 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*\ n +87 h n .t\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b * '(@ +2547 h '(@ .t\ATV.dial :waste2 F* % +108 h % .t\AS.seek 0ml F*'V +2547 h 'V #t\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T .t\ASV.dial :SPEdelivery F*  +21 h  .tdelay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial :air1 F*  +86 h F*% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' .t\ATV.dial :ASV F* n +130 h n .t\ASV.dial :SPErecovery F*  +43 h  F*% h % .t\AS.seek 0.62ml F*'\ +2033 h '\  .t\ATV.dial :waste F* n +150 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +3154 h 'R .t\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :air3 F*" % +43 h % F*(% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *+' +423 h ' .t\ATV.dial :air3 F*,% h % .t\AS.seek 0.53ml F**' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*%% h % .t\AS.seek 0.71ml F*' H +3641 h ' H .tdelay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % .tdelay 3 seconds +300 F*2% h % H .t\AS.seek 0.735ml F*'' Ű +525 h ' Ű .tdelay 3 seconds +300 .\ATV.dial :bleach F*3  +22 h F*1% h % Ű .t\AS.seek 0.72ml F*5' zG +324 h ' zG .tdelay 3 seconds +300 .\ATV.dial ASV F*4 n +86 h n .t\ASV.dial :SPErecovery F*0% h % zG .t\AS.seek 0.365ml F*6' +7161 h ' .t\ATV.dial :waste F*8 n +150 h n .t\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *7'R +1873 h 'R .t\ATV.dial :air3 F*: % +43 h % F*% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *;' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*<% h % .t\AS.seek 0.579ml F*?' +11484 h '  .tdelay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % .tdelay 3 seconds +300 F*B% h %  .t\AS.seek 0.604ml F*>'\ 48 +525 h '\ 48 .tdelay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*E% h %\ 48 .t\AS.seek 0.589ml F*C' +324 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*D n +86 h n .t\ASV.dial :wasteR F*H  +21 h  .t\AS.seek 0ml F*I'V +11871 h 'V .tdelay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *F'V +20132 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air3 F*K % +108 h % F*J% h %V .t\AS.seek 0.01ml F*L' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*M  +22 h F*P% h % .t\AS.seek 0.58ml F*N' q +11484 h ' q .tdelay 3 seconds +300 .\ATV.dial :air3 F*O % +22 h % .tdelay 3 seconds +300 F*R% h % q .t\AS.seek 0.605ml F*T'\ 8 +525 h '\ 8  .tdelay 3 seconds +300 .\ATV.dial :bleach F*Q  +22 h F*S% h %\ 8  .t\AS.seek 0.59ml F*X' +324 h '  .tdelay 3 seconds +300 .\ATV.dial ASV F*U n +86 h n .t\ASV.dial :SPEdelivery F*W  +21 h  .t\AS.seek 0ml F*Z'V +11886 h 'V .tdelay 1:00 +6000 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *Y'V +20132 h 'V .tdelay 10 seconds +1000 .\ATV.dial :air2 F*V  +65 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *^' x +8069 h ' x .tdelay 3 seconds +300 .\ATV.dial :ASV F*\\ n +65 h n .t\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *]'^ +15506 h '^ .tdelay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*_ n +151 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *b'R +202 h 'R .t\SPE.coast F*`  h n #tRemoving Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*a'(@ +2903 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ATV.dial :ASV F*e n +150 h n .t\ASV.dial :SPErecovery F*c  +43 h  F*d% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *h' +4647 h '  .t\ASV.dial :wasteD F*i I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *f'V +3704 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *g'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*k  +43 h  F*l% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *j': +2036 h ': .t\ATV.dial :air2 F*n  +65 h  F*o% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *p'Q +424 h 'Q .t\ATV.dial ASV F*q n +65 h n F*s% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *m' + +2539 h ' + .t\ASV.dial :wasteD F*r I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *v'V +2899 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*u  +43 h  F*y% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *w': +2036 h ': .t\ATV.dial :air2 F*x  +65 h  F*|% h %: .t\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *z'Q +424 h 'Q .t\ATV.dial ASV F*{ n +64 h n F*}% h %Q .t\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + .t\ASV.dial :wasteD F* I +43 h I .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *~'V +2899 h 'V .t\ATV.dial :air2 F*  +64 h  .t\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': .t\ATV.dial :ASV F* n +64 h n .t\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q .t\ATV.dial :waste F* n +151 h n .t\AS.seek 0ml F*'V +313 h 'V .t\ASV.dial :SPEdelivery F*  +86 h  #tdeconLysateINT .\ATV.dial :air3 F* % +42 h % F*\ % h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h % .t\AS.seek 0.96ml F*'0 +19126 h '0 .tdelay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % .tdelay 3 seconds +300 F*% h %0 .t\AS.seek 0.985ml F*' +526 h ' .tdelay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % .t\AS.seek 0.97ml F*'c +323 h 'c  .tdelay 3 seconds +300 .\ATV.dial AIV F* I +108 h I .t\AIV.dial :coilToMFB .\AWV.select :waste C' +6 .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +198 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *"'R +10088 h 'R .t\ATV.dial :air2 F*(  +43 h  F*% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *,' +222 h ' .t\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *+' +9988 h ' .t\ATV.dial AIV F* I +43 h I .t\AS.seek 0.02ml F*%' +9880 h ' .t\ATV.dial :waste F** n +129 h n .t\AS.seek 0ml F*/'V +227 h 'V .t\AWV.select :air C' +6 F*2% h %V #t\Cleaning PCR .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .t\ASV.dial :waste1 F*3  +21 h .t\ATV.dial :air3 F*1 % +43 h % F*'% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *4'Z +223 h 'Z .tdelay 2 seconds +200 .\ATV.dial :bleach F*0  +21 h F*5% h %Z .t\AS.seek 0.81ml F*8'> +6055 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*6 % +22 h % .tdelay 3 seconds +300 F*:% h %> .t\AS.seek 0.835ml F*'n +525 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*9% h %n .t\AS.seek 0.82ml F*<'q +324 h 'q .tdelay 3 seconds +300 .\ARV.dial :waste F*;  +32 h  .tdelay 3 seconds +300 .\ATV.dial :ARV F*=  +64 h  F*B% h %q .t\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *?'n +2037 h 'n .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*@' +3071 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2723 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V .t\ARV.dial between carrier and air1 F*I  +32 h  .t\ATV.dial :air3 F*G % +86 h % F*K% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *J' +223 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*F  +21 h F*M% h % .t\AS.seek 0.81ml F*P'> +16110 h '> .tdelay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % .tdelay 3 seconds +300 F*O% h %> .t\AS.seek 0.835ml F*R'm +525 h 'm .tdelay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*Q% h %m .t\AS.seek 0.82ml F*S'q +324 h 'q .tdelay 3 seconds +300 .\ATV.dial PCR F*T  +21 h  .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *U'R +4741 h 'R .t\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*W' +5775 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2723 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V .tdelay 3 seconds +300 .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *`'V +5060 h 'V .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*b n +87 h n .t\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *e'(@ +2547 h '(@ .t\ATV.dial :waste2 F*c % +108 h % .t\AS.seek 0ml F*d'V +2547 h 'V #t\Starting mfb #pcrBAC sampling at most 100ml, wcr at most 100ml .BACfans.suppressMail "Started mfb in tube 2 at 16:51:20CEST15-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:50:50, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 100ml, wcr at most 100ml" F*a% #tLoading sh1 puck from tube 2 into Collection Clamp h %V C'i& h & .tHand.open M&h1 +150 h 1 M&f% h %\  A(k% h %X A(l. h ., A(g& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (n& h &  M&o& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &j( +20 h ( M&q& h & A(s& h &  .t\Forearm.seek 2 + 700 counts M&p' +90 h ' A(r& h &  .t\SE.seek 20.71 Pucks below Top Plate A(m' +56 h ' A(v& h &  M&u& h & .t\Elbow.seek 2 M&y( +7 h ( M&t& h & A(x& h &  .t\Forearm.seek 2 + 300 counts M&|'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &w/ +150 h / ."downElevator"\SE.seek :down M&{& A(}'X h & M&z% h %8 .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +415 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  .t\SC.coast #collectSample 100ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S) + +7 h + S)\ % h % C'& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % #tBubblePoint: Intake @14.7psia, Outlet @14.7psia .\CBV.dial SSV C'D @ +19 h @ .t\SS.reconfigure SSconfig .\SS.seek 10ml S)#.0X9 h 9gd +100 C'D9 h 9~d +100 C'9 h 9d +100 C'I9 h 9d +100 C'G9 h 9d +100 C'K9 h 9d +100 C'H9 h 9d +100 C'C9 h 9 d +100 C'M9 h 9d +100 C'J9 h 96d +100 C'O9 h 9Md +100 C'F9 h 9dd +100 C'N9 h 9{d +100 C'@9 h 9d +100 C'P9 h 9d +100 C'L9 h 9d +100 C'Q9 h 9d +100 C'T9 h 9d +100 C'S9 h 9 d +100 C'W9 h 9 d +100 C'Z9 h 9 /d +100 C'R9 h 9 E d +100 C'X9 h 9 [d +100 C'[9 h 9 qd +100 C'Y9 h 9 d +100 C'U9 h 9 d +100 C'V9 h 9  d +100 C'^9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C']9 h 9\ 0d +100 C'c9 h 9\ !4d +100 C'e9 h 9\ 78d +100 C'a9 h 9\ M& h & A(D& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(I% h %Z ."downElevator"\SE.seek :down A(G'X +100 (K% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +89 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'M @ +2 A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'O ` +19 h ` .t\AIV.dial :bypass #Beginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'F& h & .tHand.open M&N1 +2 h 1 M&@& h & M&P% h %\  .t\Elbow.seek FlushPuck::Garage M&L(- +165 h (- M&Q& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &- .t\Forearm.seek :garage M&S'η +91 h 'η .tHand.close M&W/ +150 h / M&Z& h &- M&R% h %η .t\Forearm.seek :retract M&X'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +265 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' .t\CC.seek :closed C'^(W +416 h (W C'_& h &W .tHand.open M&b1 +150 h 1 M&]% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%e +150 h P%a% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%\\' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%h  +37 h  .t\PS.seek 0ml P%f' +1598 h ' .t\PSV.dial PSR P%k +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%`' +2609 h ' ."connect"\PRV.dial CBV P%g  .t\CBV.dial PRV C'n  h +76 .t\PSV.dial :mixloop P%o +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%l  +113 h .t\PS.seek 0ml P%q' +2610 h ' .t\PSV.dial :air P%r  +38 h  .t\PS.seek 2.5ml P%v' +2609 h ' .t\PSV.dial :mixloop P%t +38 h .t\PS.seek 0ml P%s' +2610 h ' .t\PSV.dial :air P%&  +37 h  .t\PS.seek 2.5ml P%i' +2610 h ' .t\PSV.dial :mixloop P%x +38 h .t\PS.seek 0ml P%u' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&)& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &{(ӷ +7 h (ӷ M&z& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&j' +91 h ' .tHand.close M&#/ +150 h / .t\CC.seek :open C'p( +416 h ( M&& h &ӷ M&~% h % .t\Forearm.seek :retract M&m'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &|(- +264 h (- M&y& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &}& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &.'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'A& h & M&% h %\  A(% h %X A($. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b ( ( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b & ' +90 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &!/ +150 h / ."downElevator"\SE.seek :down A('X M&"& h & M&,% h %8 .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&+(ӷ +143 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'1(W +416 h (W .tHand.open M&*1 +150 h 1 M&0% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +150 h  .t\CSV.dial CTV C'  +19 h  .t\CS.seek 5ml C'4'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C' ' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'8'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%5  +37 h  .t\PS.seek 2.5ml P%;' +2610 h ' .t\PSV.dial :mixloop P%= +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%3 +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%?  +150 h  .t\PS.seek :empty P%'' +2610 h ' .t\CBV.dial :waste1 C': @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'D @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'I  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'7 ` +19 h ` .t\CSV.dial :air C'9 @ +37 h @ .t\CS.seek 10ml C'K' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'B'4 +2647 h '4 .t\CBV.dial :waste1 C'E @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'M  +151 h C'/& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&O% h %\  A(F% h %X A(N. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (@(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (P& h &( M&& h &ӷ M&Q% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &T( +142 h ( M&G& h & A(& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(<& h &( .t\SE.seek -0.29 Pucks below Top Plate A(W'\\ +264 h '\\ M&Z& h & A(R& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &C'C +25 h 'C .t\Elbow.hold M&S, +7 h , .t\Elbow.reconfigure TightElbow A(Y- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(U' +27 h ' M&L% h %C A(X& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &6'o +21 h 'o M&_& h & A(b& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &]' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(e' +37 h ' M&a& h & M&V% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +89 h '\  .t\Elbow.seek CC M&\\(ӷ +142 h (ӷ M&k& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'o( +416 h ( A(l& h &( A(`& h &( M&r& h &ӷ M&q% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &v( +143 h ( M&&& h & A(s& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &x'C +92 h 'C .t\Elbow.hold M&i, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &)1 +150 h 1 M&z% h %C A(w& h &( .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &#'o +21 h 'o M&[& h & A(c& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &^' +22 h ' A(% h % ."downElevator"\SE.seek :down A(~'X +100 (j% h %S M&y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +90 h '\  +60 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%{  +2 A(6 +111 h  P%% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%m +38 h .t\PRV.dial :air P%A  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'  +38 h .t\PRV.dial between CBV and air P%$  +19 h  .t\PRV.dial :air P%  +18 h  P%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%. @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&|& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&\ 'η +91 h 'η .tHand.close M&!/ +150 h / M&"& h &- M&,% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  C'*& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%0 @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%%% h % .t\PS.seek 1.499ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%J' +1094 h ' .t\PSV.dial :air P%4  +38 h  .t\PS.seek 0ml P% ' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%5 +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%u  +38 h  .tdelay 2 seconds +200 P%=% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%3  +76 h .tdelay 2 seconds +200 P%?% h %r .t\PS.seek 0.475ml P%;' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%:  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%>'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%D  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%I  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %7' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%9 +75 h .t\PTV.dial :PRV,avoiding: :puck P%8 @ +75 h @ .t\PS.seek 0ml P%H' +2610 h ' .t\PSV.dial :air P%K  +75 h  .t\PS.seek 2.5ml P%E' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%M +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%/' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%O  +75 h  .t\PS.seek 2.5ml P%F' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%N +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%P  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %Q' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'G & +50 h & .t\AWV.select :waste C' +6 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %<'nC +10548 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'+ { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1312 h ' .t\PSV.dial :air P%Z  +38 h  .t\PS.seek 2.5ml P%R' +2610 h ' .t\PSV.dial :PML P%Y +37 h .t\PS.seek 0ml P%L' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'X& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'_& F*b @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*a  .t\AIV.dial :bypass .\PSV.dial PSR P%V +22 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%T' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*W I +21 h I F*C% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.3ml F*#,,'Td20 b *k': +174 .t\PSV.dial :waste1 P%f  +38 h  .t\PS.seek 0ml P%6' +1597 h ' .t\PSV.dial PSR P%U +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%]' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%S +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%n' +1480 h ': ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*h  +43 h  F*1% h %: ."MFBPCR"\AS.seek 0.33ml F*l'ї +87 h ' .t\PSV.dial PSR P%` +113 h P%r% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%q' +420 h 'ї ."MFBPCR"\ATV.dial :waste2 F*& % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *s'R +1157 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%\\' +2 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*)  +22 h  F*z% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *w' +1430 h ' ."MFBPCR"delay 3 seconds +143 h ' .t\PSV.dial PSR P%[ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%c' +113 ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*t% h % ."MFBPCR"\AS.seek 0.469ml F*v' +2454 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%j +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%y' +2610 h ' .t\PSV.dial PSR P%x +113 h P%#% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%e' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%{  +38 h  .t\PS.seek 0ml P%' +635 h ' ."MFBPCR"delay 5 seconds +500 F*^9 h 9j #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*~ n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*@% h % ."MFBPCR"\AS.seek 0.041ml F*''% +441 h ' .t\PSV.dial PSR P%m +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%A' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%$ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%.' +558 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +34 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +122 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2487 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%| +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2679 h 'k .t\PSV.dial :waste1 P%  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +106 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*i I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *!'V +954 h ' .t\PSV.dial :air P%"  +113 h  .t\PS.seek 2.5ml P%,' +2610 h ' .t\PSV.dial :mixloop P%} +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %\ ' +5106 h ' .t\PSV.dial :air P%(  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %2' +1221 h 'V ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *o'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +276 h ' .t\PSV.dial :mixloop P%* +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %0' +514 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F* 'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*  +86 h  F*%% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*4% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2775 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'5  +37 h  C'u% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'='P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C' ' +1625 h ' ."connect"\CSV.dial CTV C'3  .t\CTV.dial CSV C'?  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C':'4 +1811 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*;  +21 h ."MFBPCR"delay 3 seconds +300 F*D% h %_ ."MFBPCR"\AS.seek 0.434ml F*I'ݏ +215 h '4 .t\CSV.dial CSR C'>  +37 h  C'9% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'8'P +267 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*7  +21 h F*K% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +324 h '& ."MFBPCR"delay 3 seconds +204 h 'P .tdelay 5 seconds +96 ."MFBPCR"\ATV.dial ASV F*M n +108 h n F*E% h %& ."MFBPCR"\AS.seek 0.092ml F*O'&0 +296 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'/' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'P'4 +2647 h '4 C'& h &W #tGaraging FlushPuck in Collection Clamp M&F& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &G(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &ӷ .t\Forearm.seek CC M&+' +91 h ' .tHand.close M& n +173 h n ."MFBPCR"\ASV.dial :SPEelution F*9  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *J% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *:' ?x +2603 #"WCR"\Sampled 100.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'7  +75 h  ."WCR"delay 1:00 +622 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *K% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *I' +5071 ."WCR"\CSV.dial :air C'M @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'E' +644 h ' ."MFBPCR"delay 5 seconds +266 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'H @ +75 h @ +50 S)8% h % ."WCR"\CBV.dial between CSV and waste2b C'N  +94 h  C'/% h % ."WCR"\CSV.dial between air and CTV C' ` +15 F*F% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +4 h ` ."WCR"\CS.seek 7.288ml C'Q' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'<  '  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'p'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +996 h ' ."MFBPCR"delay 1:00 +5004 ."WCR"\CTV.dial :air,avoiding: :intake C'Y  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'+  +38 h  C'_% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'a' +607 h ' ."WCR"delay 5 seconds +195 #"MFBPCR"pushing eluate to holding coil F*G% h % ."MFBPCR"\AS.seek 0.279ml F*'Q +305 ."WCR"\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'R @ +75 h @ +50 S)C% h % ."WCR"\CBV.dial between CSV and waste2b C'f  +94 h  C'd% h % ."WCR"\CSV.dial between air and CTV C'T ` +57 h ` ."WCR"\CS.seek 7.288ml C'6' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'S  ']  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'V'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'1  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '`' +41 h ' ."WCR"delay 1:00 +91 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*k n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*& % +43 h % F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *)'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*z I +21 h I F*W% h %v ."MFBPCR"\AS.seek 0.059ml F*s'H +434 ."WCR"\CTV.dial :air,avoiding: :intake C'[  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C't  +38 h  C'h% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +3 h 'H ."MFBPCR"\ATV.dial :air3 F*r % +3 ."WCR"\CS.seek 2ml C'c' +19 h % F*x% h %H ."MFBPCR"\AS.seek 0.069ml F*j' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +22 h F*n% h % ."MFBPCR"\AS.seek 0.769ml F*' q +144 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'g' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'\\ @ +76 h @ +50 S)O% h % ."WCR"\CBV.dial between CSV and waste2b C'Z  +94 h  C'^% h % ."WCR"\CSV.dial between air and CTV C'e ` +56 h ` ."WCR"\CS.seek 7.288ml C'@' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'A'4 +1919 h '4 ."WCR"delay 2:00 +9805 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h % q ."MFBPCR"\AS.seek 0.794ml F*U' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F* I +102 ."WCR"\CBV.dial :waste2b C'  +6 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +13 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '$' +41 h ' ."WCR"delay 1:00 +4361 h ' ."MFBPCR"\ATV.dial :waste F*' n +129 h n ."MFBPCR"\AS.seek :empty F*|'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*~% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*.  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*"' +907 ."WCR"\CTV.dial :air,avoiding: :intake C'  +151 h C'}& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&,% h %\  A(i% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ((( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (!& h &( M&& h &ӷ M&(% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\ ( +143 h ( M&o& h & A(*& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A('K +253 h 'K M&& h & A(%& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b & 'C +26 h 'C ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow M&$&.,(\ #b A(4- h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&2% h %C A(5& h &( ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &B'o +20 h 'o M&u& h & A(3& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &?' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(=' +36 h ' M& & h & M&;% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  ."WCR"\Elbow.seek CC M&>(ӷ +143 h (ӷ M&9& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.seek CC M&D' +91 h ' ."WCR"Hand.close M&7/ +150 h / ."WCR"\CC.seek :open C'K( +416 h ( A(:& h &( A(M& h &( M&P& h &ӷ M&H% h % ."WCR"\Forearm.seek :retract M&8'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &N( +143 h ( M&/& h & A(F& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C ."WCR"\Elbow.hold M&I, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &E1 +150 h 1 M&<% h %C A( & h &( ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &Q'o +20 h 'o M&& h & A(p& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &Y' +22 h ' A(+% h % ."downElevator"\SE.seek :down A(_'X +100 (% h %B M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'a& h & .tHand.open M&R1 +2 A(6 h 1 M&C& h & M&f% h %\  .t\Elbow.seek FlushPuck::Garage M&d(- +166 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &L& h &- .t\Forearm.seek :garage M&S'η +91 h 'η .tHand.close M&]/ +150 h / M&6& h &- M&1% h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &&& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +90 h ' .t\CC.seek :closed C'X(W +416 h (W C'z& h &W .tHand.open M&W1 +150 h 1 M&)% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +91 h '\  #t\Flushing the Collection Valve::Manifold C't& h &W C'h% h % .t\CSV.dial CSR C'r  +38 h  C'x% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'`'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 's% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'q'[ +463 h '[ .t\CSV.dial :waste C'n +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'c& h &W C'\\% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'^ @ +38 h @ C'e% h %p .t\CS.seek 7ml C'g'i +416 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV C'  +37 h  +113  .t\CBV.dial :waste1 C'@ @ +75 h @ .t\CS.seek 0ml C'y'4 +21 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*m'\ 48 +123 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'U% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C''p +326 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*A% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*'' +2 h 'p .tdelay 5 seconds +321 h '  ."MFBPCR"delay 3 seconds +179 .t\CSV.dial :air C' @ +38 h @ C'~% h %p .t\CS.seek 7ml C''i +83 ."MFBPCR"\ATV.dial :ASV F*| n +86 h n ."MFBPCR"\ASV.dial :wasteR F*.  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +204 h 'i ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'}'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C',  +38 h  C'i% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '!' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C'( @ +75 h @ .t\CS.seek 0ml C'o'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'*  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'w'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'v' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'%  +75 h  .t\CS.seek 0ml C'4'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'2  +37 h  C'5% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'u'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C';  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'\ '4 +910 h '4 .tdelay 7 seconds +442 h 'V ."MFBPCR"delay 1:00 +258 .t\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'9'4 +507 h '4 #t\Flushing upper manifold C'J& h &W C'B% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C'?'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'7% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'{ +113 h .t\CTV.dial :air .\CS.seek 0ml C':'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'M'0 +507 h '0 .t\CSV.dial :waste C'P +37 h .t\CS.seek 0ml C'H'4 +507 h '4 C'/& h &W C'F% h %4 .t\CSV.dial :flush C'D  +37 h  .t\CS.seek 3ml C'>'0 +383 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +118 h '0 .tdelay 5 seconds +82 ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +418 .t\CSV.dial :air C'< @ +38 h @ C' % h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'p'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'+'0 +506 h '0 .t\CSV.dial :waste C'8 +76 h .t\CS.seek 0ml C'N'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'G% h %4 .t\CSV.dial :flush C'a  +38 h  .t\CS.seek 5ml C'R'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'C @ +38 h @ C'f% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV C'Q  .t\CTV.dial CSV C'=  +37 h  +113  .t\CBV.dial :waste1 C'Y @ +38 h @ .t\CS.seek 0ml C'T'4 +631 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +29 h '4 #t\Flushing lower manifold's waste2b port C'L& h &W C'% h %4 .t\CSV.dial :flush C'_  +71 ."MFBPCR"\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *I'V +4 h  .t\CS.seek 5ml C']'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +37 h @ C'1% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'S  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'&'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&d& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +7 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &z& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&)/ +150 h / .t\CC.seek :open C't( +416 h ( M&h& h &ӷ M&r% h % .t\Forearm.seek :retract M&x'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &l(- +265 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &s& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +91 h 'η .tHand.open M&n1 +150 h 1 M&q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 190515totalsampletest until completion of MFBPCR +16294 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*\\ % +107 h % F*O% h %V ."MFBPCR"\AS.seek 0.59ml F*j' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*^ n +107 h n ."MFBPCR"\ASV.dial :wasteR F*e% h %  ."MFBPCR"\AS.seek 0.09ml F*Z' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*@% h % ."MFBPCR"\AS.seek 0.12ml F*g' +625 h ' ."MFBPCR"\ATV.dial :air3 F*# % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +21 h F*% h % ."MFBPCR"\AS.seek 0.23ml F*"' +2034 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*A'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*~' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*| n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*.  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *''5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4795 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*, I +86 h I ."MFBPCR"\AS.seek 0ml F*i'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*}'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  #"MFBPCR"\Swish #1 of 2 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *('\  +1031 h '\  F*!% h %\  ."MFBPCR"\AS.seek 0.5ml F**'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*% h %(A ."MFBPCR"\AS.seek 0.6ml F*o'\  +1031 h '\  F*w% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*%% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *v' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*2 % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *5'V +3472 h 'V ."MFBPCR"\SPE.coast F*4  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0105h100ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*u  +64 h  ."MFBPCR"\ARV.dial :carrier F*;  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*\ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*J  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*B  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *9'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*7% h %Fj ."MFBPCR"\AS.seek 0.524ml F*?' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*{ % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*:' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*P  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*M% h % ."MFBPCR"\AS.seek 0.536ml F*/'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*F  +22 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*>  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*p' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*8  +43 h  ."MFBPCR"\ASV.dial :waste1 F*+  +65 h ."MFBPCR"\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*G% h % \\ ."MFBPCR"\AS.seek 0.326ml F*a'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*C % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 !o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0105h100ml.pcr F*f% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*Q  +21 h ."MFBPCR"\ATV.dial :air3 F*= % +87 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*L  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*6'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*1  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*S'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*d% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *K'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *['V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *h'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*r  +32 h  ."MFBPCR"\ATV.dial :air3 F*W % +86 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 F*#,,'Td20 b ."MFBPCR"\AS.seek 0.01ml F*s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*V  +22 h F*n% h % ."MFBPCR"\AS.seek 0.81ml F*q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*`% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*O  +22 h F*I% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*e  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0107h100ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*.  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*' I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*i n +21 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*}  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.536ml F*('< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*w'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*o n +21 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*2  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.65ml F*4' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*u  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*;  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*\ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0107h100ml.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*J  +22 h ."MFBPCR"\ATV.dial :air3 F*B % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*7% h %Z ."MFBPCR"\AS.seek 0.81ml F*9'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*?'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +21 h F*M% h %n ."MFBPCR"\AS.seek 0.82ml F*:'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*F  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*D  +65 h  F*/% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*<' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*+  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *p' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*N  +22 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*b'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*a% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*Y% h %m ."MFBPCR"\AS.seek 0.82ml F*R'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*L  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *6' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0109h100ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*K  +11 h  F*z% h %V ."MFBPCR"\AS.seek 0.5ml F*)'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *X'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *r'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*W  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*$  +22 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *V'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*n  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*s% h %Fj ."MFBPCR"\AS.seek 0.524ml F*x' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*`  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*O' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*I % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.536ml F*e'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +107 h  ."MFBPCR"delay 7 seconds +700 F*j% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*Z'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*#  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*g' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*y'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0109h100ml.pcr F*"% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*m  +21 h ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*|% h %Z ."MFBPCR"\AS.seek 0.81ml F*''> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*!  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b **'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*;% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*\ '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*B% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*7' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +5060 h 'V #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0112h100ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*/  +43 h  ."MFBPCR"\ARV.dial :carrier F*:  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*<'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*> n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*+  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*N  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*C% h %Fj ."MFBPCR"\AS.seek 0.524ml F*p' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*f  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.53ml F*b' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*=  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Y% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*L  +107 h  ."MFBPCR"delay 7 seconds +700 F*R% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*E% h %\  ."MFBPCR"\AS.seek 0.6ml F*T'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*1% h %\  ."MFBPCR"\AS.seek 0.65ml F*]' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*6  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*S'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*K  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*z % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0112h100ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*d @@T ."MFBPCR"\ASV.dial :SPEdelivery F*)  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*k  +150 h F*X% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *W' +424 h ' ."MFBPCR"\ATV.dial :ASV F*$ n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*h  +43 h  F*r% h % ."MFBPCR"\AS.seek 0.62ml F*n'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*s n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *V'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *`'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*q'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*x % +43 h % F*I% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *\\' +424 h ' ."MFBPCR"\ATV.dial :air3 F*O% h % ."MFBPCR"\AS.seek 0.53ml F*^' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*e% h % ."MFBPCR"\AS.seek 0.71ml F*' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*@ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*#' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Z% h % Ű ."MFBPCR"\AS.seek 0.72ml F*U' zG +323 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F* % h % zG ."MFBPCR"\AS.seek 0.365ml F*g' +7162 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*" % +43 h % F*y% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *m' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +21 h F*.% h % ."MFBPCR"\AS.seek 0.579ml F*' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*|% h %  ."MFBPCR"\AS.seek 0.604ml F*~'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*'' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*i'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +107 h % F*}% h %V ."MFBPCR"\AS.seek 0.01ml F**' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*o% h % ."MFBPCR"\AS.seek 0.58ml F*%' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*u  +22 h F*;% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*v' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*4 n +85 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +22 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*\  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *J' x +8070 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*B n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *5'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*7'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*{  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *9' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*P I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*F  +43 h  F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *:': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*D% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *<'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*> n +64 h n F*H% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2898 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*N% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *C': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*8  +65 h  F*f% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *a'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*p n +65 h n F*=% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *Y' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*b I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *L'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*R  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *E'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*_'V +312 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*1  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*T % +43 h % F*6% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*]% h % ."MFBPCR"\AS.seek 0.96ml F*K'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h %0 ."MFBPCR"\AS.seek 0.985ml F*S' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +21 h F*X% h % ."MFBPCR"\AS.seek 0.97ml F*['c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*$ I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *r'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *s'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *V'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*`  +43 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *I' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *q' +9988 h ' ."MFBPCR"\ATV.dial AIV F*O I +43 h I ."MFBPCR"\AS.seek 0.02ml F*\\' +9880 h ' ."MFBPCR"\ATV.dial :waste F*j n +128 h n ."MFBPCR"\AS.seek 0ml F*e'V +227 h 'V ."MFBPCR"\AWV.select :air C' +7 F*^% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*g'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*A  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*.  +65 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*|' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F*' % +87 h % F*i% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *!' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*( % +21 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F**'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*w  +21 h F*2% h %m ."MFBPCR"\AS.seek 0.82ml F*%'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *5'(@ +2548 h '(@ ."MFBPCR"\ATV.dial :waste2 F* % +107 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 190515totalsampletest assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:52:09, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 190515totalsampletest assays" #\Mission runtime was 2:26.542177 #-> exit #m\EXIT @1557932046.79CEST @1557994433.17CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =mquickScheduler =tquick =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "190516totalsampletest.rb" .GoodNews.suppressMail "Starting 190516totalsampletest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:14:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "190516totalsampletest begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/190516totalsampletest.rb:111\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:14:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:14:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 190516totalsampletest phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== C'/% h %4 M&1% S)2% h %\  % M&3& h &M A(4& h &  A(5% h %X C'6& h & + P%0% %7& h % & F*8% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +7 *9% h %&K .t\ATV.dial :waste2 F*: % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *;' +2409 h ' .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*<' +5284 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :waste F*= n +107 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *>'R +5065 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  .t\ASV.dial :waste1 F*@  h #t\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:14:50CEST16-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:14:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*A% h %R #tLoading sh1 puck from tube 2 into Collection Clamp C'B& h & .tHand.open M&C1 +150 h 1 M&D% h %\  A(E% h %X A(F. h ., A(G& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (H& h &  M&I& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &J( +20 h ( M&K& h & A(L& h &  .t\Forearm.seek 2 + 700 counts M&M' +90 h ' A(N& h &  .t\SE.seek 20.71 Pucks below Top Plate A(O' +56 h ' A(P& h &  M&Q& h & .t\Elbow.seek 2 M&R( +7 h ( M&S& h & A(T& h &  .t\Forearm.seek 2 + 300 counts M&U'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down M&W& A(X'X h & M&Y% h %8 .t\Forearm.seek :retract M&Z'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&[(ӷ +143 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C'_(W +415 h (W .tHand.open M&`1 +150 h 1 M&a% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)c+ +7 h + S)d% h % C'e& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (y( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (2& h &  M&7& h &- M&6% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &%( +166 h ( M&B& h & A(}& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &5' +90 h ' A(E& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(/' +77 h ' A(& h &  M&G& h & ."WCR"\Elbow.seek 2 M&:( +6 h ( M&L& h & A(<& h &  ."WCR"\Forearm.seek 2 + 300 counts M&P'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +150 h / ."downElevator"\SE.seek :down M&N& A(D'X h & M&3% h %8 ."WCR"\Forearm.seek :retract M&0'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&?(ӷ +143 h (ӷ M&@& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' ."WCR"\CC.seek :closed C')(W +3 ."MFBPCR"\ATV.dial :waste2 F*K % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +370 h (W ."WCR"Hand.open M&81 +150 h 1 M&T% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +39 h 'R ."MFBPCR"\ATV.dial :air2 F*9  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *Y' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)F+ +6 h + S)% h % C']& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'#  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +43 h % F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +22 h F*1% h % ."MFBPCR"\AS.seek 0.579ml F*B' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +21 h % ."MFBPCR"delay 3 seconds +300 F*E% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*G% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*L' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +86 h n ."MFBPCR"\ASV.dial :wasteR F**  +43 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +20131 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*% % +108 h % F*5% h %V ."MFBPCR"\AS.seek 0.59ml F*@' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*H n +108 h n ."MFBPCR"\ASV.dial :wasteR F*P% h %  ."MFBPCR"\AS.seek 0.09ml F*/' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*D I +86 h I F*:% h % ."MFBPCR"\AS.seek 0.12ml F*0' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*9% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h % ."MFBPCR"\AS.seek 0.255ml F*V'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*y% h %X ."MFBPCR"\AS.seek 0.24ml F*F' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*` n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *e'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*U @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*( I +85 h I ."MFBPCR"\AS.seek 0ml F*g'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*J  +42 h  #"MFBPCR"\Swish #1 of 2 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *o'\  +1032 h '\  F*h% h %\  ."MFBPCR"\AS.seek 0.5ml F*R'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*i% h %(A ."MFBPCR"\AS.seek 0.6ml F*l'\  +1031 h '\  F*j% h %\  ."MFBPCR"\AS.seek 0.5ml F*M'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*x% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *f' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*I % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3472 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0104h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*k  +64 h  ."MFBPCR"\ARV.dial :carrier F*n  +11 h  F*.% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*s n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*C  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +21 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*_ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*w  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*v'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*+ % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +254714 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'c @ +75 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'[% h % ."WCR"\CSV.dial between air and CTV C'u ` +19 h ` ."WCR"\CS.seek 7.288ml C'"' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'4  'p  +18 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C''  +150 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C';  +38 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'>' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)=% h % ."WCR"\CBV.dial between CSV and waste2b C'6  +94 h  C'1% h % ."WCR"\CSV.dial between air and CTV C'7 ` +57 h ` ."WCR"\CS.seek 7.288ml C'}' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'E  'B  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'G  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'<  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'*  +37 h  C'L% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'A' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'% @ +76 h @ +50 S)5% h % ."WCR"\CBV.dial between CSV and waste2b C'3  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C'P ` +56 h ` ."WCR"\CS.seek 7.288ml C'@' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'K  'D  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ':'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'/  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'0& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&T% h %\  A(9% h %X A(8. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (](( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (#& h &( M&& h &ӷ M&W% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +143 h ( M&V& h & A(`& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(F& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(a'K +253 h 'K M&Z& h & A(e& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &('C +26 h 'C ."WCR"\Elbow.hold M&O, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(g- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(J' +27 h ' M&!% h %C A(Y& h &( ."WCR"\Forearm.force -20 .\Forearm.seek 1 + 300 counts M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &h'o +20 h 'o M&o& h & A(i& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(j' +36 h ' M&l& h & M&x% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +89 h '\  ."WCR"\Elbow.seek CC M&I(ӷ +143 h (ӷ M&f& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&k' +91 h ' ."WCR"Hand.close M&n/ +150 h / ."WCR"\CC.seek :open C'.( +416 h ( A(^& h &( A($& h &( M&s& h &ӷ M&~% h % ."WCR"\Forearm.seek :retract M&'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +143 h ( M&\\& h & A({& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &b'C +92 h 'C ."WCR"\Elbow.hold M&_, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&t% h %C A(& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&|'o +20 h 'o M&d& h & A(w& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &S' +22 h ' A( % h % ."downElevator"\SE.seek :down A('X +100 (% h %B M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 A(6 h 1 M&& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +90 h ' .t\CC.seek :closed C',(W +416 h (W C'q& h &W .tHand.open M&[1 +150 h 1 M&u% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'p& h &W C'4% h % .t\CSV.dial CSR C'"  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ''% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ';'[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'>& h &W C'% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '6'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'1 @ +38 h @ C'7% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV C'B  +37 h  +113  .t\CBV.dial :waste1 C'} @ +75 h @ .t\CS.seek 0ml C'G'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'<% h %4 .t\CSV.dial :flush C'*  +76 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'A% h %p .t\CS.seek 7ml C'%'i +416 h 'i ."connect"\CSV.dial CTV C'5  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'3  +75 h  .t\CS.seek 0ml C'H'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'P  +38 h  C'N% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'D'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +709 h ' ."connect"\CSV.dial CTV C'@  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'/ @ +75 h @ .t\CS.seek 0ml C':'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'T'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +710 h ' ."connect"\CSV.dial CTV C'8  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'#  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'W  +37 h  C'V% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '`'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV C'? .t\CBV.dial CSV C'Q  +37 h +76 .t\CTV.dial :air C'Z  +150 h .t\CS.seek 0ml C'e'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +506 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'Y'4 +507 h '4 #t\Flushing upper manifold C'o& h &W C'i% h %4 .t\CSV.dial :flush C'l  +37 h  .t\CS.seek 5ml C'x'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +37 h @ C'y% h %p .t\CS.seek 7ml C'a'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +113 h .t\CTV.dial :air .\CS.seek 0ml C'f'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'm'0 +507 h '0 .t\CSV.dial :waste C'h +37 h .t\CS.seek 0ml C'J'4 +507 h '4 C'R& h &W C'O% h %4 .t\CSV.dial :flush C'n  +37 h  .t\CS.seek 3ml C'M'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'^% h %0 .t\CS.seek 5ml C'$'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C's'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'~'0 +506 h '0 .t\CSV.dial :waste C'\\ +76 h .t\CS.seek 0ml C'{'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'k& h &W C'I% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C't'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'd% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +112  .t\CBV.dial :waste1 C'C @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'b% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +38 h  .t\CBV.dial :waste2b C'S  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&\ & h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &.'η +91 h 'η .tHand.open M&q1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'p& h & .tHand.open M&41 +2 h 1 M&"& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&''η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&;% h %η .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &1' +90 h ' .t\CC.seek :closed C'7(W +416 h (W C'6& h &W .tHand.open M&E1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'2& h &W C'% h %4 .t\CSV.dial CSR C'<  +38 h  C'*% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'G'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'A'[ +463 h '[ .t\CSV.dial :waste C'L +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '5'4 +2010 h '4 #t\Purging external Intake C'3& h &W C'%% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'N' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H 'D  +38 h +38 .t\CTV.dial :intake C'@  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'/'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'K  +76 h #t\Flushing upper manifold C'& h &W C'0% h %4 .t\CSV.dial :flush C':  +75 h  .t\CS.seek 5ml C'T'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +38 h @ C'#% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'W +113 h .t\CTV.dial :air .\CS.seek 0ml C'V'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'` +38 h .t\CS.seek 0ml C'Q'4 +506 h '4 C'?& h &W C'Z% h %4 .t\CSV.dial :flush C'F  +38 h  .t\CS.seek 3ml C'e'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +37 h @ C'g% h %0 .t\CS.seek 5ml C'o'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +506 h '0 .t\CSV.dial :waste C'Y +75 h .t\CS.seek 0ml C'X'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&f' +90 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'm( +416 h ( M&R& h &ӷ M&O% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &J(- +265 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &M'η +91 h 'η .tHand.open M&$1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:31 #\Extending 190516totalsampletest until completion of MFBPCR +289588 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0104h1500ml.pcr F*~% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*I  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*b% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *,' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +21 h F*[% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5060 h 'V #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0106h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*1 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*G n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*A' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.536ml F*P'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*@ n +21 h n ."MFBPCR"delay 2 seconds +200 F*N% h %\  ."MFBPCR"\AS.seek 0.6ml F*K'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*#% h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0106h1500ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +86 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*l% h %n ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*a  +64 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +32 h  ."MFBPCR"\ATV.dial :air3 F*x % +86 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *s' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +21 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*v% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*{% h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *b'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0108h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +21 h ."MFBPCR"delay 2 seconds +200 F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*+  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*[ % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*"'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*c n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*.'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0108h1500ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*E % +87 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*B% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*G'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*A  +65 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *D'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +31 h  ."MFBPCR"\ATV.dial :air3 F*/ % +87 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*W% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +5059 h 'V #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0111h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*x  +22 h ."MFBPCR"delay 2 seconds +200 F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*n  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*s' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*~  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*v% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*I'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +108 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0111h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*b @@T ."MFBPCR"\ASV.dial :SPEdelivery F*S  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*|  +151 h F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :ASV F*r n +129 h n ."MFBPCR"\ASV.dial :SPErecovery F*_  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*, n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *+'V +29 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*p% h % ."MFBPCR"\AS.seek 0.71ml F*c' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*"% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*.% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*u n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*=% h % zG ."MFBPCR"\AS.seek 0.365ml F*;' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*> % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +22 h F*2% h % ."MFBPCR"\AS.seek 0.579ml F*6' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +21 h % ."MFBPCR"delay 3 seconds +300 F*B% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F**% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*1' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*L n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*G'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *A'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*5 % +108 h % F*3% h %V ."MFBPCR"\AS.seek 0.01ml F*D' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*@% h % ."MFBPCR"\AS.seek 0.58ml F*N' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*H % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*K'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*8% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*0' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*W  +21 h  ."MFBPCR"\AS.seek 0ml F*#'V +11887 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *V'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*? n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *T'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*` n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +202 h 'R ."MFBPCR"\SPE.coast F*Q  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*!'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*F n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *g' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*Y I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*(  +43 h  F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *y': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*a% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *}'Q +423 h 'Q ."MFBPCR"\ATV.dial ASV F*R n +65 h n F*7% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *O' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*^ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*f  +42 h  F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*)  +64 h  F*~% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *v'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*s n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *{' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*k  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *I': +3042 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*t n +150 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*C % +43 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*S% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*]% h % ."MFBPCR"\AS.seek 0.97ml F* 'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*r I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *,'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\ 'R +10087 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*q I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek 0ml F*p'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*"  +21 h ."MFBPCR"\ATV.dial :air3 F*c % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *.'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*;'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*2  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*D'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*H  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*N'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *T'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*Z % +108 h % ."MFBPCR"\AS.seek 0ml F*F'V +2547 h 'V #t\Asleep after completing 190516totalsampletest assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:15:37, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 190516totalsampletest assays" #\Mission runtime was 1:59.997068 @1558345170.83CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 !o:Storage::Warning:bt[ "8/home/esp/esp2/utils/storage.rb:408:in `readPucks!'"6/home/esp/esp2/utils/storage.rb:597:in `restore!'"-/home/esp/esp2/lib/esp.rb:127:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Uncleanly Exited #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9  #t-> execute "TotalWCRTest.rb" !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"^/home/esp/esp2/mission/TotalWCRTest.rb:117: syntax error, unexpected $end, expecting kEND #-> execute "TotalWCRTest.rb" .GoodNews.suppressMail "Starting TotalWCRTest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:39:38, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "TotalWCRTest begun" #\Preparing to sleep until +00:00:10 ."shutdown"\SS.coast #t\Asleep til +00:00:10 S)5 ."shutdown"\CC.coast #t\Continuing at 01:00 ."shutdown"\CS.coast C'6 E ;3\\0\\0 ."shutdown"\PC.coast .\Forearm.coast P%6 .tGoodNews.suppressMail "Then, in tube 2: wcrPhase2\\n/home/esp/esp2/mission/TotalWCRTest.rb:112\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:18, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" ."shutdown"\Elbow.coast M&5 ."shutdown"\SC.coast .\SE.coast A(6 +2 C'5 A(5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown +970 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )D h  C' 'E h  P% %F h  F* *G h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *H  h  .t\SPE.configure SPEconfig F*\ 2 *I% h %&K .t\AS.configure ASconfig F*#,,'T(^20( b S)J% h % .t\SS.configure SSconfig C'K& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started TotalWCRTest phase wcrPhase2" #\Phase [wcrPhase2] #==================> STARTED WCRSphase (phase #1) <=== #\Starting stand-alone bac WCR #bac wcr sampling at most 2000ml .BACfans.suppressMail "Started stand-alone bac WCR in tube 2 at 11:42:23CEST20-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:23, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "bac wcr sampling at most 2000ml" #Whole Cell Archive sampling 2000ml #Loading wcr puck from tube 2 into Collection Clamp C'r& h & ."WCR"Hand.open M&s1 +150 h 1 M&t% h %\  A(u% h %X A(v. h ., A(w& h &  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (x& h &  M&y& h &M ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +20 h ( M&{& h & A(|& h &  ."WCR"\Forearm.seek 2 + 700 counts M&}' +90 h ' A(~& h &  ."WCR"\SE.seek 20.71 Pucks below Top Plate A(' +56 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A( 'X M&\ % h %8 ."WCR"\Forearm.seek :retract M& '\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."WCR"\Elbow.seek CC M& (ӷ +143 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' ."WCR"\CC.seek :closed C'(W +415 h (W ."WCR"Hand.open M&1 +150 h 1 M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  ."WCR"\SC.coast #collectSample 2000ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X @ +76 h @ ."WCR"\CS.seek 10ml C'?' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'@ @ +76 h @ +50 S)A% h % ."WCR"\CBV.dial between CSV and waste2b C'B  +94 h  C'C% h % ."WCR"\CSV.dial between air and CTV C' ` +18 h ` ."WCR"\CS.seek 7.288ml C'#' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'.  ."WCR"\CSV.dial CTV C'&  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '!'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'$  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '-' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C')  +37 h  C'D% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'E' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'G @ +75 h @ +50 S)H% h % ."WCR"\CBV.dial between CSV and waste2b C'I  +94 h  C'J% h % ."WCR"\CSV.dial between air and CTV C'K ` +56 h ` ."WCR"\CS.seek 7.288ml C'L' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'N  'M  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'O'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'P  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'Q' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'R  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'S  +38 h  C'T% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'U' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'V' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'W @ +75 h @ +50 S)X% h % ."WCR"\CBV.dial between CSV and waste2b C'Y  +94 h  C'Z% h % ."WCR"\CSV.dial between air and CTV C'] ` +57 h ` ."WCR"\CS.seek 7.288ml C'^' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'_  'b  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'e'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'c  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'k' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'm  +150 h C'n& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&o% h %\  A([% h %X A(d. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (h(( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (j& M&r& h &( &ӷ ."WCR"\Elbow.reconfigure TightElbow ."raiseStack"\SE.seek :upperLimit ."WCR"\Elbow.seek CC A(s'Z M&$&.,(\ #b &t(ӷ +6 h (ӷ M&u& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ ."WCR"\Forearm.seek CC M&w' +91 h ' ."WCR"Hand.close M&x/ +150 h / ."WCR"\CC.seek :open C'y( +23 h 'Z +393 ( A({& h &( A(|& h &( M&~& h &ӷ M&\\% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &i'C +93 h 'C ."WCR"\Elbow.hold M&l, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h %C A(\ & h &( ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &f'o +21 h 'o M&z& h & A(}& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +22 h ' A(% h %Z ."downElevator"\SE.seek :down A('X +100 (% h % M& % h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&q1 + A(6 + h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +165 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&g'η +91 h 'η .tHand.close M&a/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &"& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'((W +416 h (W C'*& h &W .tHand.open M&+1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'%% h % .t\CSV.dial CSR C''  +37 h  C'/% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C','p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '2% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '3'[ +463 h '[ .t\CSV.dial :waste C'4 +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '5'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'0& h &W C'1% h %4 .t\CSV.dial :flush C'7  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '8'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C':% h %p .t\CS.seek 7ml C';'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'6  '=  +37 h  +113  .t\CBV.dial :waste1 C'> @ +76 h @ .t\CS.seek 0ml C'`'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'@& h &W C'A% h %4 .t\CSV.dial :flush C'B  +75 h  .t\CS.seek 5ml C'C'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'?% h %p .t\CS.seek 7ml C'&'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'.  +37 h  .t\CBV.dial :waste2b C'#  +76 h  .t\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing upper manifold C'!& h &W C' % h %4 .t\CSV.dial :flush C')  +75 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'- @ +38 h @ C'E% h %p .t\CS.seek 7ml C'G'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H 'I  +37 h +76 .t\CTV.dial :air C'J  +150 h .t\CS.seek 0ml C'K'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'M +37 h .t\CS.seek 0ml C'N'4 +507 h '4 C'L& h &W C'P% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.seek 3ml C'R'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'S @ +38 h @ C'T% h %0 .t\CS.seek 5ml C'Q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'U'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +507 h '0 .t\CSV.dial :waste C'X +75 h .t\CS.seek 0ml C'Y'4 +507 h '4 #tDisinfecting external Intake with mfbkill C'Z& h &W C']% h %4 .t\CSV.dial CSR C'V  +150 h  C'b% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C'_'L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'^' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'c +113 h .t\CTV.dial :intake C'e  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'm' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'n  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'o'4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'[  +113 h  C'd% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'r'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'j' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'u  'v  +37 h  +113  .t\CBV.dial :waste1 C'x @ +38 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '{'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '|' +710 h ' ."connect"\CSV.dial CTV C'~  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'\\  +76 h  .t\CS.seek 0ml C''4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 't'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'  '\ +38 h +75 .t\CTV.dial :air C'z  +151 h .t\CS.seek 0ml C'}'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C'& h &W C' % h %4 .t\CSV.dial :flush C'i  +38 h  .t\CS.seek 5ml C' 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV C'f .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 C'& h &W C'h% h %4 .t\CSV.dial :flush C'l  +38 h  .t\CS.seek 3ml C'a'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'g'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'" +75 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'y% h %4 .t\CSV.dial :flush C'*  +38 h  .t\CS.seek 5ml C'+'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'%'i +415 h 'i ."connect"\CSV.dial CTV C''  .t\CTV.dial CSV C'/  +38 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C'k'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'2& h &W C',% h %4 .t\CSV.dial :flush C'4  +75 h  .t\CS.seek 5ml C'3'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +38 h @ C'1% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CTV C'5  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'9  +76 h  .t\CS.seek 0ml C':'4 +766 h '4 #tDisinfecting external Intake with kill C'8& h &W C'6% h %4 .t\CSV.dial CSR C'=  +37 h  C'>% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C';'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'@' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'A  'B +38 h +75 .t\CTV.dial :intake C'`  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'?  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'C'4 +607 h '4 .t\CSV.dial CSR C'.  +113 h  C'#% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'&'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C')'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&$& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &-(ӷ +6 h (ӷ M&E& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &D& h &ӷ .t\Forearm.seek CC M&I' +91 h ' .tHand.close M&H/ +150 h / .t\CC.seek :open C'J( +416 h ( M&G& h &ӷ M&K% h % .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &F(- +265 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &O'η +91 h 'η .tHand.open M&N1 +150 h 1 M&S% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &T'\ +90 h '\  #t\Completed [wcrPhase2] begun 01:00:41 #\Asleep after completing TotalWCRTest assays .GoodNews.suppressMail "{1 => 1, 2 => 21, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:26, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing TotalWCRTest assays" #\Mission runtime was 26.146806 seconds #-> execute "190517totalsampletest.rb" .GoodNews.suppressMail "Starting 190517totalsampletest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:42:26, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "190517totalsampletest begun" #\Preparing to sleep until +0:00:15 ."shutdown"\SS.coast S)5 ."shutdown"\CC.coast #t\Asleep til +0:00:15 ."shutdown"\CS.coast C'6 #t\Continuing at 03:28 ."shutdown"\PC.coast E ;3\\0\\0 ."shutdown"\Forearm.coast P%6 ."shutdown"\Elbow.coast M&5 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/190517totalsampletest.rb:111\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:15, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 03:28" +2 C'5 M&6 +28 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown +1470 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )R h  C' 'Q h  P% %U h  F* *X h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ *\ 9\ .t\SPE.coast F*W  h  .t\SPE.configure SPEconfig F*\ 2 *Z% h %&K .t\AS.configure ASconfig S)]% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'V& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 190517totalsampletest phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== C'q% h %4 M& % S)f% h %\  % M&& h &M A(s& h &  A(% h %X C' & h & +2 P%% %& h % & F*h% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *l% h %&K .t\ATV.dial :waste2 F* % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*a n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*g  +11 h  .t\ASV.dial :waste1 F*"  h #t\Starting mfb #pcrBAC sampling at most 1000ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 11:45:21CEST20-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:45:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1500ml" F*% h %R #tLoading sh1 puck from tube 2 into Collection Clamp C'& h & .tHand.open M&y1 +150 h 1 M&*% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (+& h &  M&'& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &/( +21 h ( M&& h & A(%& h &  .t\Forearm.seek 2 + 700 counts M&2' +89 h ' A(,& h &  .t\SE.seek 20.71 Pucks below Top Plate A(4' +57 h ' A(k& h &  M&0& h & .t\Elbow.seek 2 M&1( +6 h ( M&3& h & A(5& h &  .t\Forearm.seek 2 + 300 counts M&9'8 +23 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &7/ +150 h / ."downElevator"\SE.seek :down M&8& A(6'X h & M&=% h %8 .t\Forearm.seek :retract M&>'\ +56 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&:(ӷ +143 h (ӷ M&;& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &B' +91 h ' .t\CC.seek :closed C'`(W +416 h (W .tHand.open M&@1 +150 h 1 M&?% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1000ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S).+ +6 h + S)#% h % C'C& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % #t\Pulling 1.5ml of CSR.lysis .\CSR.select :lysis C' +7 .t\CS.reconfigure CSconfig .\CS.seek 1.5ml C'#P d@b '' +478 h '  .tdelay 5 seconds +500 .\CSR.select :air C' +6 '@% h %  .t\CS.seek 4.5ml C'?'` +860 h '` .tdelay 2 seconds +200 .\CSV.dial CTV C'#  +38 h  .t\CS.seek 1.275ml C'C' +919 h ' #tHeating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 .\CH.seek 85C,8:00 C'B  w '9 h 9d +100 C'w9 h 9d +100 C'.9 h 94d +100 C'!9 h 9N d +100 C' 9 h 9gd +100 C'$9 h 9d +100 C'E9 h 9d +100 C'D9 h 9d +100 C'&9 h 9 d +100 C'9 h 9$d +100 C'9 h 9(d +100 C'(9 h 9,d +100 C'H9 h 910d +100 C'K9 h 9J4d +100 C'I9 h 9c8d +100 C'L9 h 9|9 h 9 (d .t\CH.hold 5:00,85C C'6  w ( h  +30000  C'@9 h 9 w #t\Collection Puck Heater at 85C after lysing .\CS.seek 0ml C''4 +422 h '4 .t\CTV.dial between spare and air,avoiding: :intake C'# @ +113 h @ .t\CSV.dial :air C'? @ +38 h @ .t\CS.seek 5ml C''p +1372 h 'p .t\CSV.dial CTV C'w  +37 h  .tdelay 2 seconds +200 .\CSV.dial :air C'. @ +38 h @ .tdelay 2 seconds +200 .\CSV.dial CTV C'!  +38 h  .tdelay 2 seconds +200 .\CSV.dial :air C' @ +37 h @ .tdelay 2 seconds +200 .\CS.seek 0ml C'$'4 +1373 h '4 #tRecovering Lysate into Collection syringe .\CSV.dial CBV C'E +112 h .t\CBV.dial CSV C'D  +19 h C'&% h %4 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CTV.dial :air C'  +38 h C'(% h %p .t\CS.seek 7ml C'H'i +607 h 'i .tdelay 5 seconds +500 .\CSV.dial :air2 C'K @ +37 h @ .t\CS.seek 10ml C'I' +860 h ' .t\CSV.dial between air and CTV,avoiding: CBV C'L ` +132 h ` #tLysing Complete C'P& h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&-% h %\  A(G% h %X A(M. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (O(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (<& M&F& h &( &ӷ ."raiseStack"\SE.seek :upperLimit .t\Elbow.reconfigure TightElbow A(N'Z .t\Elbow.seek CC M&$&.,(\ #b &S(ӷ +7 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &)& h &ӷ .t\Forearm.seek CC M&J' +91 h ' .tHand.close M&R/ +150 h / .t\CC.seek :open C'Q( +23 h 'Z +393 ( A(T& h &( A(X& h &( M&W& h &ӷ M&V% h % .t\Forearm.seek :retract M&]'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&Y& h & A(Z& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &_'C +92 h 'C .t\Elbow.hold M&c, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &^1 +150 h 1 M&n% h %C A(m& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&['o +20 h 'o M&o& h & A(U& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &b' +22 h ' A(r% h %Z ."downElevator"\SE.seek :down A(u'X +100 (x% h % M&e% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +81 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'{' +6 h ' .t\CSV.dial between air and CTV C'v ` +19 h ` .t\AIV.dial :bypass C'~ { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'\\& h & .tHand.open M&}1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&j(- +166 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +90 h 'η .tHand.close M&|/ +150 h / M&& h &- M&q% h %η .t\Forearm.seek :retract M&f'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +265 h (ӷ M&\ & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M& 1 +150 h 1 M&h% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &l'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +150 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%a' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%g' +1598 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%  C'y  h +76 .t\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +113 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%*' +2609 h ' .t\PSV.dial :mixloop P%+ +38 h .t\PS.seek 0ml P%' +2610 h ' .t\PSV.dial :air P%%  +37 h  .t\PS.seek 2.5ml P%k' +2610 h ' .t\PSV.dial :mixloop P%' +38 h .t\PS.seek 0ml P%3' +2609 h ' #tGaraging FlushPuck in Collection Clamp M&"& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &0(ӷ +7 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&7/ +150 h / .t\CC.seek :open C'z( +416 h ( M&=& h &ӷ M&8% h % .t\Forearm.seek :retract M&t'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +264 h (- M&2& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &;'η +91 h 'η .tHand.open M&41 +150 h 1 M&9% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &1'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'>& h & M&6% h %\  A(@% h %X A(C. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (#( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (?& h &  M&& h &- M&w% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &.( +166 h ( M&!& h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(E& h &  .t\SE.seek 19.71 Pucks below Top Plate A(D' +66 h ' A(&& h &  M&$& h & .t\Elbow.seek 2 M&( +6 h ( M&(& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&K'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &B/ +150 h / ."downElevator"\SE.seek :down M&H& A(L'X h & M&P% h %8 .t\Forearm.seek :retract M&-'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&G(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &F' +91 h ' .t\CC.seek :closed C'p(W +416 h (W .tHand.open M&)1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &:'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +150 h  .t\CSV.dial CTV C'`  +19 h  .t\CS.seek 5ml C'T'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'X' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'W'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%V  +37 h  .t\PS.seek 2.5ml P%Y' +2610 h ' .t\PSV.dial :mixloop P%Z +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%J  +37 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%S +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%^  +150 h  .t\PS.seek :empty P%n' +2610 h ' .t\CBV.dial :waste1 C'm @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'o  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'U @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'r  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'] ` +19 h ` .t\CSV.dial :air C' @ +37 h @ .t\CS.seek 10ml C'x' +2647 h ' .t\CSV.dial CTV C'e  +38 h  .t\CS.seek 0ml C'_'4 +2647 h '4 .t\CBV.dial :waste1 C'[ @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'N& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&v% h %\  A(~% h %X A(\\. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (}(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (& h &( M&& h &ӷ M&b% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &d( +142 h ( M& & h & A(u& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &O' +90 h ' A(I& h &( .t\SE.seek -0.29 Pucks below Top Plate A(c'\\ +264 h '\\ M&|& h & A(& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &q'C +26 h 'C .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(\ ' +27 h ' M&% h %C A(j& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&f'o +20 h 'o M&Q& h & A( & h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(h' +37 h ' M&& h & M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&g' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'y( +416 h ( A(& h &( A(& h &( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &+( +143 h ( M&*& h & A(%& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C .t\Elbow.hold M&', +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &k1 +150 h 1 M&"% h %C A(,& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts + M&5'o +21 h 'o M&7& h & A(s& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &l' +22 h ' A(% h % ."downElevator"\SE.seek :down A(='X +100 (8% h %S M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +90 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%A  + A(6 +111 h  P%0% h % .t\PS.seek 0.499ml P%4' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%9 +38 h .t\PRV.dial :air P%t  +37 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%>  +38 h .t\CBV.dial AIV C'6  +38 h .t\PRV.dial between CBV and air P%@  +19 h  .t\PRV.dial :air P%C  +18 h  P%?% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %' +251 h ' .t\PRV.dial :waste2 P%w @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %;' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'!& h & .tHand.open M& 1 +2 h 1 M&E& h & M&/% h %\  .t\Elbow.seek FlushPuck::Garage M&&(- +166 h (- M&$& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&1/ +150 h / M&B& h &- M&H% h %η .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &.(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &<' +91 h ' .t\CC.seek :closed C'K(W +416 h (W C'D& h &W .tHand.open M&L1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  C'z& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%) @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%R  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +76 h  .tdelay 2 seconds +200 P%`% h % .t\PS.seek 1.499ml P%F' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%#' +1094 h ' .t\PSV.dial :air P%{  +38 h  .t\PS.seek 0ml P%T' +2609 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%X +38 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%V  +38 h  .tdelay 2 seconds +200 P%3% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %Z'r +2309 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%J  +76 h .tdelay 2 seconds +200 P%S% h %r .t\PS.seek 0.475ml P%^' +2562 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%Y  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%m'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%o  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%U  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %r' +1632 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%] +75 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%W' +2610 h ' .t\PSV.dial :air P%e  +75 h  .t\PS.seek 2.5ml P%x' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%[ +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P% ' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%N  +75 h  .t\PS.seek 2.5ml P%v' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%~ +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%\\  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'b & +50 h & .t\AWV.select :waste C' +7 P% % h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %u'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'I { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %G' +1313 h ' .t\PSV.dial :air P%:  +37 h  .t\PS.seek 2.5ml P%|' +2610 h ' .t\PSV.dial :PML P% +37 h .t\PS.seek 0ml P%' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 '& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'j& F*Q @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W F*  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P% +21 h  ."MFBPCR"delay 20 seconds +91 h P%% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +7 .t\PS.seek 1.5ml P%O' +1597 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*d I +129 h I F*c% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *q'6 +66 .t\PSV.dial :waste1 P%a  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%f +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%\ ' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +112 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +22 h ' .t\PSV.dial PSR P% +21 h  F*p% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +37 h  .t\PS.seek 0ml P%g' +107 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*  +21 h  F*k% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *"' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%, +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%7' +217 ."MFBPCR"\ATV.dial :AIV F*s I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%+ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial PSR P% +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%5' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%h  +38 h  .t\PS.seek 0ml P%A' +739 h ' ."MFBPCR"delay 5 seconds +500 F*09 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*l n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*=% h % ."MFBPCR"\AS.seek 0.041ml F*}'% +336 h ' .t\PSV.dial PSR P%n +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%9' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%t +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%>' +2609 h ' .t\PSV.dial PSR P%6 +113 h P%@% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%C' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%?  +38 h  .t\PS.seek 0ml P%4' +661 h '% ."MFBPCR"delay 10 seconds +936 h ' .t\PSV.dial PSR P%w +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +20 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +2382 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %E'k +2679 h 'k .t\PSV.dial :waste1 P%/  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %$' +211 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*( I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *''V +849 h ' .t\PSV.dial :air P%1  +113 h  .t\PS.seek 2.5ml P%B' +2610 h ' .t\PSV.dial :mixloop P%H +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %2' +5106 h ' .t\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*M  +43 h  F*&% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *P'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*y I +43 h I F*D% h %Q ."MFBPCR"\AS.seek 0.01ml F*L' +171 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %z' +619 h ' ."MFBPCR"\ATV.dial :waste F*) n +128 h n ."MFBPCR"\AS.seek :empty F*<'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*R  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *`' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*;  +43 h F*F% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *{'_ +2671 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'#  +37 h  C'X% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C'V'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'3' +1625 h ' ."connect"\CSV.dial CTV C'T  .t\CTV.dial CSV C'J  +38 h  +113  .t\CBV.dial :waste1 C'S @ +38 h @ .t\CS.seek 0ml C'Z'4 +1915 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*Y  +22 h ."MFBPCR"delay 3 seconds +300 F*^% h %_ ."MFBPCR"\AS.seek 0.434ml F*o'ݏ +110 h '4 .t\CSV.dial CSR C'U  +37 h  C'm% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 4ml C']'P +371 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +22 h F*r% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*e'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*W n +107 h n F*[% h %& ."MFBPCR"\AS.seek 0.092ml F*x'&0 +192 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'N' +1625 h ' ."connect"\CSV.dial CTV C'  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'~'4 +2647 h '4 C'\\& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &v(ӷ +7 h (ӷ M&b& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&I/ +150 h / .t\CC.seek :open C'u( +416 h ( M&:& h &ӷ M&G% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &|(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'd& h & M&c% h %\  A(a% h %X A(. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (O( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (f& h &  M&& h &- M& % h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &\ ( +166 h ( M&& h & A(p& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +90 h ' A(& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(q' +76 h ' A(& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +7 h ( M&%& h & A(& h &  ."WCR"\Forearm.seek 2 + 300 counts M&k'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down A(,'X M&*& h & M&s% h %8 ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&g(ӷ +143 h (ӷ M&"& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &7' +91 h ' ."WCR"\CC.seek :closed C'(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +369 h (W ."WCR"Hand.open M&h1 +150 h 1 M&5% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*l  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *=' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +7 h + S)n% h % C'A& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C't  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 S)?% h %5J ."WCR"\SSV.dial :spit S)C +151 h ."WCR"\SS.seek 0ml S)w' +216 h ' ."WCR"\SSV.dial CBV S)4  +150 h  ."WCR"\CTV.dial :intake C'}  +75 h  #"WCR"Waiting for external sampler to finish priming... .delayUntil "05:31:19.64" +18622 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*' +9317 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +207 C'/% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 *?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *C'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*9 @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*} I +86 h I ."MFBPCR"\AS.seek 0ml F* 'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*='(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*/  +43 h  #"MFBPCR"\Swish #1 of 2 F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *('\  +1032 h '\  F*!% h %\  ."MFBPCR"\AS.seek 0.5ml F*1'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*$% h %(A ."MFBPCR"\AS.seek 0.6ml F*H'\  +1032 h '\  F*E% h %\  ."MFBPCR"\AS.seek 0.5ml F*2'(B +1032 h '(B ."MFBPCR"delay 3 seconds +300 F*M% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *&' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*' % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *B'V +3472 h 'V ."MFBPCR"\SPE.coast F*  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0106h1000ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*D  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *R'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*y  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*;  +21 h ."MFBPCR"delay 2 seconds +200 F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *P'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*` I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*X% h %Fj ."MFBPCR"\AS.seek 0.524ml F*#' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*V % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.53ml F*_' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*S  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*3% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +22 h  ."MFBPCR"delay 7 seconds +700 F*U% h %< ."MFBPCR"\AS.seek 0.594ml F*m'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*Y n +22 h n ."MFBPCR"delay 2 seconds +200 F*Z% h %\  ."MFBPCR"\AS.seek 0.6ml F*{'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*W' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*o  +42 h  ."MFBPCR"\ASV.dial :waste1 F*e  +65 h ."MFBPCR"\ARV.dial :air1 F*[  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*]  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +254714 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'b  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C'\\ @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +910 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'. @ +75 h @ +50 S) % h % ."WCR"\CBV.dial between CSV and waste2b C'K  +94 h  C':% h % ."WCR"\CSV.dial between air and CTV C'- ` +19 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'G  '  +19 h  +57  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'v'4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'j' +40 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'c% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'f' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'd @ +75 h @ +50 S)a% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C' % h % ."WCR"\CSV.dial between air and CTV C' ` +57 h ` ."WCR"\CS.seek 7.288ml C'p' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'  ."WCR"\CSV.dial CTV C'  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '|' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'  +38 h  C'%% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'\ @ +76 h @ +50 S)% h % ."WCR"\CBV.dial between CSV and waste2b C's  +94 h  C'+% h % ."WCR"\CSV.dial between air and CTV C'k ` +56 h ` ."WCR"\CS.seek 7.288ml C'"' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C',  '  +18 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'q  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'u' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'x  +151 h C'& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&5% h %\  A(l% h %X A(h. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (A(( +200 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (t& h &( M&n& h &ӷ M&8% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &O( +142 h ( M&7& h & A(@& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &6' +90 h ' A(& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(?'K +253 h 'K M&>& h & A(C& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm .\Forearm.seek 1 M&$ ,(#b &#Q@2^d^2b &}'C +26 h 'C ."WCR"\Elbow.hold M&4, +6 h , ."WCR"\Elbow.reconfigure TightElbow A( - M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(/' +27 h ' M&w% h %C A(=& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&!'o +20 h 'o M&(& h & A($& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(E' +37 h ' M&H& h & M&M% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +89 h '\  ."WCR"\Elbow.seek CC M&'(ӷ +143 h (ӷ M&&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&D' +90 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(B& h &( A(& h &( M&L& h &ӷ M&)% h % ."WCR"\Forearm.seek :retract M&R'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +143 h ( M&;& h & A(F& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'C +92 h 'C ."WCR"\Elbow.hold M&`, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &X1 +150 h 1 M&P% h %C A(V& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&T'o +21 h 'o M&#& h & A(S& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &3' +22 h ' A(_% h % ."downElevator"\SE.seek :down A(^'X +100 (U% h %B M&J% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Y'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'Z& h & .tHand.open M&m1 +2 A(6 h 1 M&r& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&{(- +166 h (- M&o& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &e& h &- .t\Forearm.seek :garage M&['η +91 h 'η .tHand.close M& / +150 h / M&W& h &- M&]% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &b(ӷ +264 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &.' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'K& h &W .tHand.open M&:1 +150 h 1 M&-% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &I'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'G% h % .t\CSV.dial CSR C'  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'v'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'c +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'j'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'd% h %4 .t\CSV.dial :flush C'a  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ' 'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'f'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'p @ +75 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'%'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'| @ +37 h @ C'% h %p .t\CS.seek 7ml C'\ 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C's  +75 h  .t\CS.seek 0ml C'+'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'k  +38 h  C'*% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ',' +709 h ' ."connect"\CSV.dial CTV C'"  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste1 C'q @ +75 h @ .t\CS.seek 0ml C''4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'x  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '5'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'l' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +37 h  .t\CBV.dial :waste2b C't  +76 h  .t\CS.seek 0ml C'n'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'8  +38 h  C'7% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '@'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'0 'g  +38 h +75 .t\CTV.dial :air C'>  +151 h .t\CS.seek 0ml C'C'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C' '0 +506 h '0 .t\CSV.dial :waste C'w +38 h .t\CS.seek 0ml C'='4 +506 h '4 #t\Flushing upper manifold C'(& h &W C'$% h %4 .t\CSV.dial :flush C'H  +38 h  .t\CS.seek 5ml C'M'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'O% h %p .t\CS.seek 7ml C'?'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'} +113 h .t\CTV.dial :air .\CS.seek 0ml C'&'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'/'4 +507 h '4 C'1& h &W C'4% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'2'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'B% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'L'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C')'0 +506 h '0 .t\CSV.dial :waste C'; +76 h .t\CS.seek 0ml C'F'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'D& h &W C''% h %4 .t\CSV.dial :flush C'X  +38 h  .t\CS.seek 5ml C'P'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +38 h @ C'#% h %p .t\CS.seek 7ml C'S'i +416 h 'i ."connect"\CSV.dial CTV C'_  .t\CTV.dial CSV C'R  +37 h  +113  .t\CBV.dial :waste1 C'y @ +38 h @ .t\CS.seek 0ml C'U'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'J& h &W C'Z% h %4 .t\CSV.dial :flush C'm  +75 h  .t\CS.seek 5ml C'r'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV C'E  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'3  +75 h  .t\CS.seek 0ml C'o'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&e& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +7 h (ӷ M&A& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.seek CC M& ' +90 h ' .tHand.close M&W/ +150 h / .t\CC.seek :open C']( +416 h ( M&Y& h &ӷ M&[% h % .t\Forearm.seek :retract M&\\'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &~(- +265 h (- M&{& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&K1 +150 h 1 M&:% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&G1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &u& h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&v/ +150 h / M&c& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &d(ӷ +264 h (ӷ M&a& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'(W +416 h (W C' & h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &p'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'f& h &W C'% h %4 .t\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '|% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'% +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2010 h '4 #t\Purging external Intake C's& h &W C'\ % h %4 .t\CSV.dial :flush C'k  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '*' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'+  ' +38 h +37 .t\CTV.dial :intake C'"  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'q'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C',  +75 h #t\Flushing upper manifold C'x& h &W C'% h %4 .t\CSV.dial :flush C'  +76 h  .t\CS.seek 5ml C'5'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'h @ +38 h @ C't% h %p .t\CS.seek 7ml C'l'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'8 +113 h .t\CTV.dial :air .\CS.seek 0ml C'7'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'n'0 +507 h '0 .t\CSV.dial :waste C'@ +38 h .t\CS.seek 0ml C'g'4 +506 h '4 C'0& h &W C'>% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C'C'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C' % h %0 .t\CS.seek 5ml C'('p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'H'0 +507 h '0 .t\CSV.dial :waste C'= +75 h .t\CS.seek 0ml C'6'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&O& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &M(ӷ +6 h (ӷ M&}& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &?& h &ӷ .t\Forearm.seek CC M&&' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C'( +416 h ( M&1& h &ӷ M&4% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &/(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &B& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &2'η +90 h 'η .tHand.open M&1 +150 h 1 M&L% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &;'\ +91 h '\  #t\Completed [mbaPhase] begun 03:29:10 #\Extending 190517totalsampletest until completion of MFBPCR +289589 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0106h1000ml.pcr F*)% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*'  +22 h ."MFBPCR"\ATV.dial :air3 F*X % +87 h % F*F% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *V'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*#  +21 h F*P% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +21 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*m'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*U  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *r'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*W  +32 h  ."MFBPCR"\ATV.dial :air3 F*b % +87 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*[% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*^'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *G'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *~'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0108h1000ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ARV.dial :carrier F*j  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*o% h %Fj ."MFBPCR"\AS.seek 0.524ml F*|' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*s  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*\ % h % ."MFBPCR"\AS.seek 0.536ml F*k'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*+% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*" n +21 h n ."MFBPCR"delay 2 seconds +200 F**% h %\  ."MFBPCR"\AS.seek 0.6ml F*,'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*x  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*q  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*h  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*t% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*8  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*l % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0108h1000ml.pcr F*7% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *@'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*n  +22 h ."MFBPCR"\ATV.dial :air3 F*0 % +86 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*C % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*=  +22 h F*H% h %n ."MFBPCR"\AS.seek 0.82ml F*O'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*}  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*?  +64 h  F*!% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*p' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*B  +32 h  ."MFBPCR"\ATV.dial :air3 F*M % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *L' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*2% h % ."MFBPCR"\AS.seek 0.81ml F*Q'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*N% h %> ."MFBPCR"\AS.seek 0.835ml F*''m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*F% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*#  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *P'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*V' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0110h1000ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ARV.dial :carrier F*r  +10 h  F*E% h %V ."MFBPCR"\AS.seek 0.5ml F*3'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *T' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*e n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *W'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*b  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*I  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *Y'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*[  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*{' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*K' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*: % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*^% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\\  +107 h  ."MFBPCR"delay 7 seconds +700 F*G% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*. n +21 h n ."MFBPCR"delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*v  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*-  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*a% h % \\ ."MFBPCR"\AS.seek 0.326ml F*j'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0110h1000ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*]  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*o % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*|% h %n ."MFBPCR"\AS.seek 0.82ml F*s'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *+'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*k' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *"'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F*q % +87 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*8% h % ."MFBPCR"\AS.seek 0.81ml F*l'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*9 % +22 h % ."MFBPCR"delay 3 seconds +300 F*7% h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*0% h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*@  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *g'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*w' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +5059 h 'V #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0113h1000ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*!  +43 h  ."MFBPCR"\ARV.dial :carrier F*O  +11 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*p'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*1 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *4'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*B  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*M  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*2% h %Fj ."MFBPCR"\AS.seek 0.524ml F*L' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*)  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*N% h % ."MFBPCR"\AS.seek 0.53ml F*Q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*X  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*F% h % ."MFBPCR"\AS.seek 0.536ml F*''< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*#  +108 h  ."MFBPCR"delay 7 seconds +700 F*D% h %< ."MFBPCR"\AS.seek 0.594ml F*P'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*V n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*y'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*_  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.65ml F*Z' \\ +5045 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*S  +42 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*U  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*m'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*r  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*E % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0113h1000ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*3  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*T  +151 h F*A% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *b' +423 h ' ."MFBPCR"\ATV.dial :ASV F*I n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*`  +43 h  F*W% h % ."MFBPCR"\AS.seek 0.62ml F*['\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F*{ % +43 h % F*:% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *^' +424 h ' ."MFBPCR"\ATV.dial :air3 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*\\' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*G  +21 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*.' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*u % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*v' Ű +526 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +21 h F*% h % Ű ."MFBPCR"\AS.seek 0.72ml F*~' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*- n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*a% h % zG ."MFBPCR"\AS.seek 0.365ml F*' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*f% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F* ' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*% n +86 h n ."MFBPCR"\ASV.dial :wasteR F*|  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*s% h %V ."MFBPCR"\AS.seek 0.01ml F*+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*"% h % ."MFBPCR"\AS.seek 0.58ml F**' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*x% h % q ."MFBPCR"\AS.seek 0.605ml F*,'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +21 h F*h% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*8  +21 h  ."MFBPCR"\AS.seek 0ml F*t'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *7'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*l  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *n' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*0 n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *5'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*@ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *>'R +202 h 'R ."MFBPCR"\SPE.coast F*g  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*w'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*C  +43 h  F*(% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*= I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *H'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*}  +42 h  F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *O': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*d  +64 h  F*?% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *p'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*1 n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *4' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*B I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *2': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*)% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *N'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*L n +65 h n F*X% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *F' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*Q I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *#'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*D  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *'': +3042 h ': ."MFBPCR"\ATV.dial :ASV F*V n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *R'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*P n +150 h n ."MFBPCR"\AS.seek 0ml F*_'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*J  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*y % +43 h % F*S% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *U' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.96ml F*r'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %0 ."MFBPCR"\AS.seek 0.985ml F*m' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +22 h F*A% h % ."MFBPCR"\AS.seek 0.97ml F*e'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*I I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *W'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b * 'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *Y'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*{% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *:' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b * ' +9988 h ' ."MFBPCR"\ATV.dial AIV F*K I +43 h I ."MFBPCR"\AS.seek 0.02ml F*^' +9880 h ' ."MFBPCR"\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*\\% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*. % +43 h % F*c% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*v  +22 h F*-% h %Z ."MFBPCR"\AS.seek 0.81ml F*a'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*f  +64 h  F*]% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\ ' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*k % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h %> ."MFBPCR"\AS.seek 0.835ml F*+'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*x% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*q  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *h'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *n'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*g n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *5'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*> % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 190517totalsampletest assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@11:46:07, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 190517totalsampletest assays" #\Mission runtime was 2:00.260727 #-> exit #m\EXIT @1558346640.05CEST @1558360727.90CEST = =ECan =tquick =hcore =mquickScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refiH ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 16:30:07CET01-Jan-70 .delayUntil "16:30:07.00" +5580699 #-> execute "190517totalsampletest.rb" .GoodNews.suppressMail "Starting 190517totalsampletest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:58:55, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "190517totalsampletest begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 16:30 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/190517totalsampletest.rb:111\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:59:40, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 16:30" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:59:43, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 190517totalsampletest phase mbaPhase" #\Phase [mbaPhase] #==================> STARTED MBAsuperphase (phase #1) <=== C'/% h %4 M&1% S)2% h %\  % M&3& h &M A(4& h &  A(5% h %X C'6& h & + P%0% %7& h % & F*8% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +7 *9% h %&K .t\ATV.dial :waste2 F*: % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *;' +2409 h ' .t\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.seek 1ml F*<' +5284 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :waste F*= n +107 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *>'R +5065 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  .t\ASV.dial :waste1 F*@  h #t\Starting mfb #pcrBAC sampling at most 1000ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:59:45CEST20-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:59:43, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1500ml" F*A% #tLoading sh1 puck from tube 2 into Collection Clamp h %R C'B& h & .tHand.open M&C1 +150 h 1 M&D% h %\  A(E% h %X A(F. h ., A(G& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (H& h &  M&I& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &J( +20 h ( M&K& h & A(L& h &  .t\Forearm.seek 2 + 700 counts M&M' +90 h ' A(N& h &  .t\SE.seek 20.71 Pucks below Top Plate A(O' +56 h ' A(P& h &  M&Q& h & .t\Elbow.seek 2 M&R( +7 h ( M&S& h & A(T& h &  .t\Forearm.seek 2 + 300 counts M&U'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &V/ +150 h / ."downElevator"\SE.seek :down A(W'X M&X& h & M&Y% h %8 .t\Forearm.seek :retract M&Z'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&[(ӷ +143 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &]& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &^' +91 h ' .t\CC.seek :closed C'_(W +415 h (W .tHand.open M&`1 +150 h 1 M&a% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &b'\ +91 h '\  .t\SC.coast A(6 #tcollectSample 1000ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)c+ +7 h + S)d% h % C'e& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (y( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (2& h &  M&7& h &- M&6% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &%( +166 h ( M&B& h & A(}& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &5' +89 h ' A(E& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(/' +77 h ' A(& h &  M&G& h & ."WCR"\Elbow.seek 2 M&:( +7 h ( M&L& h & A(<& h &  ."WCR"\Forearm.seek 2 + 300 counts M&P'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &A/ +150 h / ."downElevator"\SE.seek :down M&D& A(N'X h & M&3% h %8 ."WCR"\Forearm.seek :retract M&0'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&?(ӷ +142 h (ӷ M&@& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &V' +91 h ' ."WCR"\CC.seek :closed C')(W +3 ."MFBPCR"\ATV.dial :waste2 F*K % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *X'R +370 h (W ."WCR"Hand.open M&81 +150 h 1 M&T% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Q'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*9  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *Y' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)F+ +7 h + S)% h % C']& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'#  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X % +43 h % F*2% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *7' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*6  +21 h F*1% h % ."MFBPCR"\AS.seek 0.579ml F*B' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*E% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*G% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*L' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*< n +86 h n ."MFBPCR"\ASV.dial :wasteR F**  +42 h  ."MFBPCR"\AS.seek 0ml F*A'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *3'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*% % +108 h % F*5% h %V ."MFBPCR"\AS.seek 0.59ml F*@' +11886 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*H n +107 h n ."MFBPCR"\ASV.dial :wasteR F*P% h %  ."MFBPCR"\AS.seek 0.09ml F*/' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*K  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*N I +86 h I F*:% h % ."MFBPCR"\AS.seek 0.12ml F*0' +626 h ' ."MFBPCR"\ATV.dial :air3 F* % +21 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*8' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*9% h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*] % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h % ."MFBPCR"\AS.seek 0.255ml F*V'X +525 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*y% h %X ."MFBPCR"\AS.seek 0.24ml F*F' +323 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*` n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*W  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Z'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *a% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *e'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*U @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*( I +86 h I ."MFBPCR"\AS.seek 0ml F*g'V +5771 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*Y'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*J  +43 h  #"MFBPCR"\Swish #1 of 2 F*!% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *o'\  +1032 h '\  F*h% h %\  ."MFBPCR"\AS.seek 0.5ml F*R'(A +1031 h '(A #"MFBPCR"\Swish #2 of 2 F*i% h %(A ."MFBPCR"\AS.seek 0.6ml F*l'\  +1032 h '\  F*j% h %\  ."MFBPCR"\AS.seek 0.5ml F*M'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*x% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *f' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*I % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *^'V +3473 h 'V ."MFBPCR"\SPE.coast F*m  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0119h1000ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*k  +64 h  ."MFBPCR"\ARV.dial :carrier F*n  +10 h  F*.% h %V ."MFBPCR"\AS.seek 0.5ml F*$'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*s n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*C  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*\\  +22 h ."MFBPCR"delay 2 seconds +200 F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*_ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*d' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F* ' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*S% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +65 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*v'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*+ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +254714 #"WCR"\Sampled 1500.0ml #Drying puck before fixing for 4:00 .\CBV.dial :waste2b C'  +75 h  ."WCR"delay 1:00 +6000 .\CSV.dial :air C' @ +38 h @ ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +909 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'c @ +76 h @ +50 S),% h % ."WCR"\CBV.dial between CSV and waste2b C'q  +94 h  C'[% h % ."WCR"\CSV.dial between air and CTV C'u ` +18 h ` ."WCR"\CS.seek 7.288ml C'"' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air C'4  ."WCR"\CSV.dial CTV C'p  +19 h  +56  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"\CBV.dial :waste2b C'  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C''  +151 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 .\CSV.dial CSR C';  +37 h  C' % h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'>' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '2' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)=% h % ."WCR"\CBV.dial between CSV and waste2b C'6  +94 h  C'1% h % ."WCR"\CSV.dial between air and CTV C'7 ` +56 h ` ."WCR"\CS.seek 7.288ml C'}' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'B  'E  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'G  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'<  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'*  +38 h  C'L% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'A' +607 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'% @ +75 h @ +50 S)5% h % ."WCR"\CBV.dial between CSV and waste2b C'3  +94 h  C'H% h % ."WCR"\CSV.dial between air and CTV C'P ` +57 h ` ."WCR"\CS.seek 7.288ml C'@' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air C'K  ."WCR"\CSV.dial CTV C'N  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ':'4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'/  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'  +150 h C'0& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&T% h %\  A(9% h %X A(8. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (](( +201 h (( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (#& h &( M&& h &ӷ M&X% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &y( +142 h ( M&V& h & A(`& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &W' +89 h ' A(F& h &( ."WCR"\SE.seek 0.71 Pucks below Top Plate A(a'K +254 h 'K M&Z& h & A(e& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &('C +25 h 'C ."WCR"\Elbow.hold M&O, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(g- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(J' +27 h ' M&!% h %C A(Y& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&h'o +21 h 'o M&o& h & A(i& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(j' +36 h ' M&l& h & M&x% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +90 h '\  ."WCR"\Elbow.seek CC M&I(ӷ +142 h (ӷ M&f& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ ."WCR"\Forearm.seek CC M&k' +91 h ' ."WCR"Hand.close M&n/ +150 h / ."WCR"\CC.seek :open C'.( +416 h ( A(^& h &( A($& h &( M&s& h &ӷ M&~% h % ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&\\& h & A({& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &b'C +93 h 'C ."WCR"\Elbow.hold M&_, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&t% h %C A(& h &( ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &|'o +21 h 'o M&d& h & A(w& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &S' +22 h ' A( % h % ."downElevator"\SE.seek :down A('X +100 (% h %B M&% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\ '\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 + A(6 + h 1 M&& h & M& % h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&+'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C',(W +416 h (W C'q& h &W .tHand.open M&[1 +150 h 1 M&u% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &r'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'p& h &W C'4% h % .t\CSV.dial CSR C'"  +38 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ''% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ';'[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'>& h &W C'% h %4 .t\CSV.dial :flush C'=  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '6'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'1 @ +38 h @ C'7% h %p .t\CS.seek 7ml C'2'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'E  'B  +38 h  +113  .t\CBV.dial :waste1 C'} @ +75 h @ .t\CS.seek 0ml C'G'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'<% h %4 .t\CSV.dial :flush C'*  +75 h  .t\CS.seek 5ml C'L'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'A% h %p .t\CS.seek 7ml C'%'i +416 h 'i ."connect"\CSV.dial CTV C'5  .t\CTV.dial CSV +38 h  .t\CBV.dial :waste2b C'3  +75 h  .t\CS.seek 0ml C'H'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'P  +38 h  C'D% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'N'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'K' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'@  +38 h  .t\CBV.dial :waste1 C'/ @ +75 h @ .t\CS.seek 0ml C':'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +37 h  C'0% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'T'P +1115 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'8  +38 h  .t\CBV.dial :waste2b C'#  +75 h  .t\CS.seek 0ml C''4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'X  +38 h  C'V% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '`'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'F' +710 h ' ."connect"\CSV.dial CBV C'? .t\CBV.dial CSV C'Q  +38 h +75 .t\CTV.dial :air C'Z  +151 h .t\CS.seek 0ml C'e'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'g'0 +507 h '0 .t\CSV.dial :waste C'! +38 h .t\CS.seek 0ml C'Y'4 +506 h '4 #t\Flushing upper manifold C'o& h &W C'i% h %4 .t\CSV.dial :flush C'l  +38 h  .t\CS.seek 5ml C'x'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'W @ +38 h @ C'y% h %p .t\CS.seek 7ml C'a'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( +113 h .t\CTV.dial :air .\CS.seek 0ml C'f'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'm'0 +506 h '0 .t\CSV.dial :waste C'h +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C'R& h &W C'O% h %4 .t\CSV.dial :flush C'n  +38 h  .t\CS.seek 3ml C'M'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C') @ +38 h @ C'^% h %0 .t\CS.seek 5ml C'$'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C's'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'~'0 +507 h '0 .t\CSV.dial :waste C'\\ +75 h .t\CS.seek 0ml C'{'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'k& h &W C'I% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 5ml C't'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'd% h %p .t\CS.seek 7ml C'w'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +113  .t\CBV.dial :waste1 C'C @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'& h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'b% h %p .t\CS.seek 7ml C'|'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +37 h  .t\CBV.dial :waste2b C'S  +76 h  .t\CS.seek 0ml C''4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M& & h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&]& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &_& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&\ & h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &+& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &.'η +91 h 'η .tHand.open M&q1 +150 h 1 M&[% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &u'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'p& h & .tHand.open M&41 +2 h 1 M&"& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&''η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&;% h %η .t\Forearm.seek :retract M&>'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &1' +91 h ' .t\CC.seek :closed C'7(W +416 h (W C'6& h &W .tHand.open M&E1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'2& h &W C'% h %4 .t\CSV.dial CSR C'<  +37 h  C'*% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'G'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'A'[ +463 h '[ .t\CSV.dial :waste C'L +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '5'4 +2009 h '4 #t\Purging external Intake C'3& h &W C'%% h %4 .t\CSV.dial :flush C'P  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'H  'N +37 h +38 .t\CTV.dial :intake C'@  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'/'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'K  +75 h #t\Flushing upper manifold C'& h &W C'0% h %4 .t\CSV.dial :flush C':  +75 h  .t\CS.seek 5ml C'T'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'8 @ +37 h @ C'#% h %p .t\CS.seek 7ml C'9'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'X +113 h .t\CTV.dial :air .\CS.seek 0ml C'V'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'Q'4 +507 h '4 C'?& h &W C'Z% h %4 .t\CSV.dial :flush C'F  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'o'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'l'0 +507 h '0 .t\CSV.dial :waste C'Y +75 h .t\CS.seek 0ml C'W'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &x(ӷ +7 h (ӷ M&(& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &a& h &ӷ .t\Forearm.seek CC M&f' +91 h ' .tHand.close M&h/ +150 h / .t\CC.seek :open C'm( +416 h ( M&R& h &ӷ M&O% h % .t\Forearm.seek :retract M&n'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &J(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &M'η +91 h 'η .tHand.open M&$1 +150 h 1 M&s% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &\\'\ +91 h '\  #t\Completed [mbaPhase] begun 16:30:38 #\Extending 190517totalsampletest until completion of MFBPCR +289589 !"PCR"o:#AnalyticalModule::PCRRawSample: @refiH ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0119h1000ml.pcr F*~% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*I  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*d  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*b% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*r% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *,' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*\  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*+% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*q  +22 h F*[% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*p  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *4'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5060 h 'V #"MFBPCR"\Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0121h1000ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*>'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*6 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *E'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b **'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*1 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*G n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*L% h % ."MFBPCR"\AS.seek 0.53ml F*A' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.536ml F*P'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*5  +108 h  ."MFBPCR"delay 7 seconds +700 F*H% h %< ."MFBPCR"\AS.seek 0.594ml F*N'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*@ n +22 h n ."MFBPCR"delay 2 seconds +200 F*D% h %\  ."MFBPCR"\AS.seek 0.6ml F*K'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.65ml F*:' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*8  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*#% h % \\ ."MFBPCR"\AS.seek 0.326ml F*T'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*9 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Pp Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Pp completed -- closed /var/log/esp/Pp70jan0121h1000ml.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*? % +87 h % F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*g'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +21 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Y  +21 h F*l% h %n ."MFBPCR"\AS.seek 0.82ml F*y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*a  +65 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*}' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*^  +31 h  ."MFBPCR"\ATV.dial :air3 F*x % +87 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *s' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +22 h F*M% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +21 h % ."MFBPCR"delay 3 seconds +300 F*v% h %> ."MFBPCR"\AS.seek 0.835ml F*I'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*{% h %m ."MFBPCR"\AS.seek 0.82ml F*k'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*d  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *t'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *b'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0123h1000ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*j% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ @CET82822.23 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*r  +22 h ."MFBPCR"delay 2 seconds +200 F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*,% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*+  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*q' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*[ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*p'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*"'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*c n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*.'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*=% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0123h1000ml.pcr F*>% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *6'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*E % +86 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*<  +22 h F*B% h %Z ."MFBPCR"\AS.seek 0.81ml F*1'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F**% h %> ."MFBPCR"\AS.seek 0.835ml F*G'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*A  +64 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *H'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*P' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *N'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*K  +32 h  ."MFBPCR"\ATV.dial :air3 F*/ % +86 h % F*8% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*:  +22 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*9'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*U % +22 h % ."MFBPCR"delay 3 seconds +300 F*V% h %> ."MFBPCR"\AS.seek 0.835ml F*T'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*`  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Q'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +5060 h 'V #"MFBPCR"\Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0202h1000ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*}'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *O'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*^  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*x  +22 h ."MFBPCR"delay 2 seconds +200 F*$% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *)'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*n  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*M% h %Fj ."MFBPCR"\AS.seek 0.524ml F*s' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*~  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*v% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.536ml F*I'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +107 h  ."MFBPCR"delay 7 seconds +700 F*k% h %< ."MFBPCR"\AS.seek 0.594ml F*t'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*C'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*w  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F* % h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Mm Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Mm completed -- closed /var/log/esp/Mm70jan0202h1000ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*b @@T ."MFBPCR"\ASV.dial :SPEdelivery F*S  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*|  +150 h F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b * ' +424 h ' ."MFBPCR"\ATV.dial :ASV F*r n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*_  +42 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*'\ +2034 h '\  ."MFBPCR"\ATV.dial :waste F*, n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *\ 'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *+'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *' +424 h ' ."MFBPCR"\ATV.dial :air3 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +22 h F*p% h % ."MFBPCR"\AS.seek 0.71ml F*c' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*"% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*.% h % Ű ."MFBPCR"\AS.seek 0.72ml F*' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*u n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*=% h % zG ."MFBPCR"\AS.seek 0.365ml F*;' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *''R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*> % +43 h % F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*E  +21 h F*2% h % ."MFBPCR"\AS.seek 0.579ml F*6' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*B% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F**% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*1' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*L n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +21 h  ."MFBPCR"\AS.seek 0ml F*G'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *A'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*5 % +108 h % F*3% h %V ."MFBPCR"\AS.seek 0.01ml F*H' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +22 h F*@% h % ."MFBPCR"\AS.seek 0.58ml F*D' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*N % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*K'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*8% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*0' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*: n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*X  +21 h  ."MFBPCR"\AS.seek 0ml F*#'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *V'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*9  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*? n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *T'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*` n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *Z'R +201 h 'R ."MFBPCR"\SPE.coast F*Q  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*F n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*e  +43 h  F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *g' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*Y I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *l'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*(  +43 h  F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *y': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*a% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *}'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*R n +65 h n F*7% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *O' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*^ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *x'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *$'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*f  +43 h  F*n% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *M': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*)  +65 h  F*~% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *v'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*s n +64 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *{' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*k  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *I': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*t n +151 h n ."MFBPCR"\AS.seek 0ml F* 'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +85 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*C % +43 h % F*w% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +21 h % ."MFBPCR"delay 3 seconds +300 F*S% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*|  +22 h F*]% h % ."MFBPCR"\AS.seek 0.97ml F* 'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*r I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *,'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *\ 'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*+  +43 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *[' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *' +9988 h ' ."MFBPCR"\ATV.dial AIV F*q I +43 h I ."MFBPCR"\AS.seek 0.02ml F*' +9880 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek 0ml F*p'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*"  +22 h ."MFBPCR"\ATV.dial :air3 F*c % +43 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *.'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*='> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*;'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*''q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*2  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *<'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*B' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +86 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *%' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*A'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*H'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*/  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *0'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Q n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *T'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*Z % +107 h % ."MFBPCR"\AS.seek 0ml F*F'V +2548 h 'V #t\Asleep after completing 190517totalsampletest assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:00:31, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 190517totalsampletest assays" #\Mission runtime was 1:59.712185 #-> execute TotalWCRTest.rb !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"(uninitialized constant TotalWCRTest: name:TotalWCRTest #-> execute "TotalWCRTest.rb" .GoodNews.suppressMail "Starting TotalWCRTest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:00:31, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "TotalWCRTest begun" #\Preparing to sleep until +00:00:10 ."shutdown"\AS.coast .\SS.coast F*5 #t\Asleep til +00:00:10 S)5 ."shutdown"\CC.coast #t\Continuing at 05:31 ."shutdown"\CS.coast C'6 E ;3\\0\\0 ."shutdown"\PC.coast .\PS.coast P%6 ."shutdown"\Forearm.coast .tGoodNews.suppressMail "Then, in tube 2: wcrPhase2\\n/home/esp/esp2/mission/TotalWCRTest.rb:112\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:10:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 05:31" M&5 ."shutdown"\Elbow.coast + P%5 M&6 C'5 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown +970 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )e h  C' 'o h  P% %! h  F* *Y h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *g  h  .t\SPE.configure SPEconfig F*\ 2 *l% h %V .t\AS.configure ASconfig S)(% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'h& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:10:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started TotalWCRTest phase wcrPhase2" #\Phase [wcrPhase2] #==================> STARTED WCRSphase (phase #1) <=== #\Starting stand-alone bac WCR #bac wcr sampling at most 2000ml .BACfans.suppressMail "Started stand-alone bac WCR in tube 2 at 16:10:29CEST20-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:10:29, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "bac wcr sampling at most 2000ml" #Whole Cell Archive sampling 2000ml #Loading wcr puck from tube 2 into Collection Clamp C']& h & ."WCR"Hand.open M&1 +150 h 1 M&r% h %\  A( % h %X A(_. h ., A(& h &  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&,& h &M ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +21 h ( M&+& h & A(& h &  ."WCR"\Forearm.seek 2 + 700 counts M&\ ' +89 h ' A([& h &  ."WCR"\SE.seek 20.71 Pucks below Top Plate A(q' +57 h ' A(& h &  M&4& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&& h & A(p& h &  ."WCR"\Forearm.seek 2 + 300 counts M&"'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &c/ +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&% h %8 ."WCR"\Forearm.seek :retract M&u'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."WCR"\Elbow.seek CC M&.(ӷ +143 h (ӷ M&& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +91 h ' ."WCR"\CC.seek :closed C'>(W +416 h (W ."WCR"Hand.open M&1 +150 h 1 M&;% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  ."WCR"\SC.coast A(6 #"WCR"collectSample 2000ml w/bubble point of 55psi #\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)2+ +6 h + S)% h % C''& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'p'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'w  +38 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV C' .t\CBV.dial CSV C'  +38 h +75 .t\CTV.dial :air C'\  +150 h .t\CS.seek 0ml C''4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +507 h '0 .t\CSV.dial :waste C'4 +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C' & h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'|'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C';% h %p .t\CS.seek 7ml C''i +415 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :air .\CS.seek 0ml C'r'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'= +38 h .t\CS.seek 0ml C''4 +506 h '4 C'"& h &W C' % h %4 .t\CSV.dial :flush C'c  +38 h  .t\CS.seek 3ml C'2'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +38 h @ C'B% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'< +75 h .t\CS.seek 0ml C'''4 +507 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'+% h %4 .t\CSV.dial :flush C'L  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'3 @ +37 h @ C'P% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'A  '5  +38 h  +113  .t\CBV.dial :waste1 C'1 @ +37 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'/& h &W C'H% h %4 .t\CSV.dial :flush C'8  +76 h  .t\CS.seek 5ml C'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C':% h %p .t\CS.seek 7ml C'X'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  +37 h  .t\CBV.dial :waste2b C'#  +76 h  .t\CS.seek 0ml C'U'4 +765 h '4 #tDisinfecting external Intake with kill C'0& h &W C'b% h %4 .t\CSV.dial CSR C'V  +38 h  C'?% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C'K'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'Q' +416 h ' ."connect"\CSV.dial CBV C'` .t\CBV.dial CSV C'Z  +37 h +76 .t\CTV.dial :intake C'd  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'J' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'T'4 +606 h '4 .t\CSV.dial CSR C'm  +113 h  C'-% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'W'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'&' +1372 h ' .t\CSV.dial CBV C'\\ +113 h .t\CS.seek 0ml C'e'4 +2648 h '4 #tGaraging FlushPuck in Collection Clamp M&z& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &F(ӷ +6 h (ӷ M&!& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &o& h &ӷ .t\Forearm.seek CC M&l' +91 h ' .tHand.close M&(/ +150 h / .t\CC.seek :open C'h( +416 h ( M&g& h &ӷ M&i% h % .t\Forearm.seek :retract M&a'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &Y(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&y1 +150 h 1 M&O% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #t\Completed [wcrPhase2] begun 05:32:00 #\Asleep after completing TotalWCRTest assays .GoodNews.suppressMail "{1 => 1, 2 => 21, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:10:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing TotalWCRTest assays" #\Mission runtime was 26.474552 seconds #-> exit #m\EXIT @1558361483.44CEST @1558361875.04CEST = =mquickScheduler =tquick =ECan =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refiܗ; : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #Loading /home/esp/esp2/mission/TotalWCRTest.rb mission .GoodNews.suppressMail "Starting TotalWCRTest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:02, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "TotalWCRTest begun" #\Preparing to sleep until +00:00:10 #"shutdown"\Shutdown #t\Asleep til +00:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: wcrPhase2\\n/home/esp/esp2/mission/TotalWCRTest.rb:112\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:03, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:07, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started TotalWCRTest phase wcrPhase2" #\Phase [wcrPhase2] #==================> STARTED WCRSphase (phase #1) <=== #\Starting stand-alone bac WCR #bac wcr sampling at most 2000ml .BACfans.suppressMail "Started stand-alone bac WCR in tube 2 at 16:18:07CEST20-May-19\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:07, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "bac wcr sampling at most 2000ml" #Whole Cell Archive sampling 2000ml #Loading wcr puck from tube 2 into Collection Clamp C'/& h & ."WCR"Hand.open M&01 +150 h 1 M&1% h %\  A(2% h %X A(3. h ., A(4& h &  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (5& h &  M&6& h &M ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &7( +20 h ( M&8& h & A(9& h &  ."WCR"\Forearm.seek 2 + 700 counts M&:' +90 h ' A(;& h &  ."WCR"\SE.seek 20.71 Pucks below Top Plate A(<' +56 h ' A(=& h &  M&>& h & ."WCR"\Elbow.seek 2 M&?( +7 h ( M&@& h & A(A& h &  ."WCR"\Forearm.seek 2 + 300 counts M&B'8 +22 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &C/ +150 h / ."downElevator"\SE.seek :down A(D'X M&E& h & M&F% h %8 ."WCR"\Forearm.seek :retract M&G'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  ."WCR"\Elbow.seek CC M&H(ӷ +143 h (ӷ M&I& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' ."WCR"\CC.seek :closed C'L(W +415 h (W ."WCR"Hand.open M&M1 +150 h 1 M&N% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &O'\ +91 h '\  ."WCR"\SC.coast #collectSample 2000ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)P+ +7 h + S)Q% h % C'R& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X( +143 h ( M&@& h & A(A& h &( ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &&'C +92 h 'C ."WCR"\Elbow.hold M&), +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &C1 +150 h 1 M&E% h %C A(F& h &( ."WCR"\Forearm.force -20 M&5 ."WCR"\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation + &#'o +21 h 'o M&7& h & A(:& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &I' +22 h ' A(J% h %Z ."downElevator"\SE.seek :down A(B'X +100 (<% h % M&D% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &?'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'G& h & .tHand.open M&.1 + A(6 + h 1 M&M& h & M&N% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&R& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&$'η +90 h 'η .tHand.close M&!/ +150 h / M&P& h &- M&U% h %η .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &V(ӷ +265 h (ӷ M&W& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &X& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'Z(W +416 h (W C'[& h &W .tHand.open M&\\1 +150 h 1 M&Y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'_& h &W C'`% h % .t\CSV.dial CSR C'a  +37 h  C'b% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'e% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'f'[ +463 h '[ .t\CSV.dial :waste C'g +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'h'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'c& h &W C'd% h %4 .t\CSV.dial :flush C'j  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'k'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'm% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'i  'p  +37 h  +113  .t\CBV.dial :waste1 C'q @ +76 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C's& h &W C't% h %4 .t\CSV.dial :flush C'u  +75 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'w @ +38 h @ C'r% h %p .t\CS.seek 7ml C'z'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'y  +37 h  .t\CBV.dial :waste2b C'x  +76 h  .t\CS.seek 0ml C'|'4 +766 h '4 #t\Flushing upper manifold C'{& h &W C'~% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' '  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'\ '4 +506 h '4 C' & h &W C' % h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C''0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tDisinfecting external Intake with mfbkill C'& h &W C'% h %4 .t\CSV.dial CSR C'  +150 h  C'% h %4 #t\Pulling 8ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 8ml C''L +815 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +113 h .t\CTV.dial :intake C'"  +75 h  .tIntake.select :open S) +6 .t\CS.seek 2ml C'*' +816 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'+  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ','4 +606 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +113 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.seek 4ml C'/'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'2  '3  +37 h  +113  .t\CBV.dial :waste1 C'5 @ +38 h @ .t\CS.seek 0ml C'H'4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'O  +37 h  C'L% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '8'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '9' +710 h ' ."connect"\CSV.dial CTV C';  .t\CTV.dial CSV +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'@'4 +909 h '4 #tKilling upper manifold .\CSV.dial CSR C'A  +38 h  C'4% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '0'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +709 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'E 'F  +38 h +75 .t\CTV.dial :air C'7  +151 h .t\CS.seek 0ml C':'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'J'0 +507 h '0 .t\CSV.dial :waste C'= +38 h .t\CS.seek 0ml C'>'4 +506 h '4 #t\Flushing upper manifold C'<& h &W C'D% h %4 .t\CSV.dial :flush C'&  +38 h  .t\CS.seek 5ml C'G'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'. @ +38 h @ C'M% h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CBV C'# .t\CBV.dial CSV +113 h .t\CTV.dial :air .\CS.seek 0ml C'1'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'I'0 +506 h '0 .t\CSV.dial :waste C'R +38 h .t\CS.seek 0ml C'K'4 +506 h '4 C'B& h &W C'%% h %4 .t\CSV.dial :flush C')  +38 h  .t\CS.seek 3ml C'!'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'P @ +37 h @ C'U% h %0 .t\CS.seek 5ml C'?'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'$'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'W'0 +506 h '0 .t\CSV.dial :waste C'X +75 h .t\CS.seek 0ml C'Q'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'T& h &W C'6% h %4 .t\CSV.dial :flush C'[  +38 h  .t\CS.seek 5ml C'\\'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Y @ +38 h @ C'_% h %p .t\CS.seek 7ml C'`'i +416 h 'i ."connect"\CSV.dial CTV C'a  .t\CTV.dial CSV C'b  +37 h  +113  .t\CBV.dial :waste1 C'S @ +38 h @ .t\CS.seek 0ml C'('4 +766 h '4 #t\Flushing lower manifold's waste2b port C'e& h &W C']% h %4 .t\CSV.dial :flush C'g  +75 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'c @ +38 h @ C'd% h %p .t\CS.seek 7ml C'j'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'h  +38 h  .t\CBV.dial :waste2b C'l  +75 h  .t\CS.seek 0ml C'm'4 +766 h '4 #tDisinfecting external Intake with kill C'k& h &W C'i% h %4 .t\CSV.dial CSR C'p  +37 h  C'q% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +7 .t\CS.seek 8ml C'n'L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C's' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'u  't +38 h +75 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C'w' +815 h ' .tIntake.select :close S)0 +7 .t\CTV.dial :air C'r  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'v'4 +607 h '4 .t\CSV.dial CSR C'y  +113 h  C'x% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'z'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'{' +1372 h ' .t\CSV.dial CBV C'~ +113 h .t\CS.seek 0ml C''4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M& & h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&\ 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Completed [wcrPhase2] begun 01:00:26 #\Asleep after completing TotalWCRTest assays .GoodNews.suppressMail "{1 => 1, 2 => 21, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@16:18:10, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing TotalWCRTest assays" #\Mission runtime was 25.409265 seconds #m\EXIT @1558361908.37CEST